AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene056082


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056082.t1 MTR_1g083580 91.589 107 8 1 1 107 176 281 2.81e-67 203
MS.gene056082.t1 MTR_7g112550 55.752 113 39 3 2 107 175 283 6.19e-36 124
MS.gene056082.t1 MTR_7g112560 37.037 108 49 4 2 107 167 257 4.21e-12 60.5
MS.gene056082.t1 MTR_1g079460 58.182 55 21 2 55 107 212 266 4.41e-12 60.8
MS.gene056082.t1 MTR_1g079380 37.037 108 53 5 3 107 170 265 8.04e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene056082.t1 AT1G09155 42.735 117 54 7 2 107 166 280 3.73e-19 80.1
MS.gene056082.t1 AT1G56250 43.966 116 54 6 2 107 167 281 1.58e-16 72.8
MS.gene056082.t1 AT1G56240 45.217 115 52 6 2 106 167 280 1.82e-16 72.8

Find 30 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGTCAAACATATTTATTT+TGG 0.292667 1.4:+67576957 None:intergenic
CAAATTGATAGAAGATAATA+AGG 0.294509 1.4:-67577135 MS.gene056082:CDS
ATATTCGAGTTCCATCTAAA+AGG 0.307761 1.4:-67577112 MS.gene056082:CDS
ATGGCCAACAAAGTGCAAAA+TGG 0.309663 1.4:-67577230 MS.gene056082:CDS
GGAGGTTAAGATTAGTGTTA+TGG 0.327028 1.4:-67577033 MS.gene056082:CDS
TGGGTGGATGGAAATTGAAT+TGG 0.398175 1.4:-67577084 MS.gene056082:CDS
AAGATTAGTGTTATGGAGGT+TGG 0.420611 1.4:-67577026 MS.gene056082:CDS
TGGCCAACAAAGTGCAAAAT+GGG 0.432183 1.4:-67577229 MS.gene056082:CDS
AAGGGTGGTCTCATCGTTGA+AGG 0.481480 1.4:-67576993 MS.gene056082:CDS
TATTCGAGTTCCATCTAAAA+GGG 0.481762 1.4:-67577111 MS.gene056082:CDS
GGGTGGATGGAAATTGAATT+GGG 0.484481 1.4:-67577083 MS.gene056082:CDS
GTTCCATCTAAAAGGGATGA+TGG 0.488515 1.4:-67577104 MS.gene056082:CDS
GGAGGTTGGTTATAGACTGA+AGG 0.490832 1.4:-67577012 MS.gene056082:CDS
GGTTAAGATTAGTGTTATGG+AGG 0.508835 1.4:-67577030 MS.gene056082:CDS
TTATGAAAAGCATAGAAAGA+TGG 0.529657 1.4:-67577165 MS.gene056082:CDS
ATTGCAACTGATACCTCAGC+TGG 0.529997 1.4:+67577251 None:intergenic
AAGGTTTCACACCGTATGTA+CGG 0.553117 1.4:-67577287 None:intergenic
TTCCATCTAAAAGGGATGAT+GGG 0.560247 1.4:-67577103 MS.gene056082:CDS
AGCTGAGGTATCAGTTGCAA+TGG 0.570422 1.4:-67577249 MS.gene056082:CDS
TAAAAGGGATGATGGGTGGA+TGG 0.573430 1.4:-67577096 MS.gene056082:CDS
GAGGTTGGTTATAGACTGAA+GGG 0.594688 1.4:-67577011 MS.gene056082:CDS
GAAAAGCATAGAAAGATGGA+AGG 0.602899 1.4:-67577161 MS.gene056082:CDS
ACTTGATTCTGCGCCAGCTG+AGG 0.612928 1.4:-67577264 MS.gene056082:CDS
CAACAAAGTGCAAAATGGGA+TGG 0.622257 1.4:-67577225 MS.gene056082:CDS
CATCTAAAAGGGATGATGGG+TGG 0.630515 1.4:-67577100 MS.gene056082:CDS
TTTCTGTGGTGAAGTTGACA+TGG 0.640269 1.4:-67577054 MS.gene056082:CDS
CTGTGGTGAAGTTGACATGG+AGG 0.689911 1.4:-67577051 MS.gene056082:CDS
TTTGTACAATAAATACGAGG+AGG 0.700943 1.4:-67577198 MS.gene056082:CDS
GTTGGTTATAGACTGAAGGG+TGG 0.721602 1.4:-67577008 MS.gene056082:CDS
ATATTTGTACAATAAATACG+AGG 0.779887 1.4:-67577201 MS.gene056082:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTTGTACAATAAATACG+AGG - chr1.4:67577042-67577061 MS.gene056082:CDS 20.0%
!! CAAATTGATAGAAGATAATA+AGG - chr1.4:67577108-67577127 MS.gene056082:CDS 20.0%
!! TTATGAAAAGCATAGAAAGA+TGG - chr1.4:67577078-67577097 MS.gene056082:CDS 25.0%
TTTGTACAATAAATACGAGG+AGG - chr1.4:67577045-67577064 MS.gene056082:CDS 30.0%
! ATATTCGAGTTCCATCTAAA+AGG - chr1.4:67577131-67577150 MS.gene056082:CDS 30.0%
! TATTCGAGTTCCATCTAAAA+GGG - chr1.4:67577132-67577151 MS.gene056082:CDS 30.0%
AAGATTAGTGTTATGGAGGT+TGG - chr1.4:67577217-67577236 MS.gene056082:CDS 35.0%
GGAGGTTAAGATTAGTGTTA+TGG - chr1.4:67577210-67577229 MS.gene056082:CDS 35.0%
GGTTAAGATTAGTGTTATGG+AGG - chr1.4:67577213-67577232 MS.gene056082:CDS 35.0%
TTCCATCTAAAAGGGATGAT+GGG - chr1.4:67577140-67577159 MS.gene056082:CDS 35.0%
! GAAAAGCATAGAAAGATGGA+AGG - chr1.4:67577082-67577101 MS.gene056082:CDS 35.0%
ATGGCCAACAAAGTGCAAAA+TGG - chr1.4:67577013-67577032 MS.gene056082:CDS 40.0%
CAACAAAGTGCAAAATGGGA+TGG - chr1.4:67577018-67577037 MS.gene056082:CDS 40.0%
GGGTGGATGGAAATTGAATT+GGG - chr1.4:67577160-67577179 MS.gene056082:CDS 40.0%
GTTCCATCTAAAAGGGATGA+TGG - chr1.4:67577139-67577158 MS.gene056082:CDS 40.0%
TGGCCAACAAAGTGCAAAAT+GGG - chr1.4:67577014-67577033 MS.gene056082:CDS 40.0%
TGGGTGGATGGAAATTGAAT+TGG - chr1.4:67577159-67577178 MS.gene056082:CDS 40.0%
TTTCTGTGGTGAAGTTGACA+TGG - chr1.4:67577189-67577208 MS.gene056082:CDS 40.0%
! CATCCCATTTTGCACTTTGT+TGG + chr1.4:67577020-67577039 None:intergenic 40.0%
! GAATTGGGTGAGTTTTTCTG+TGG - chr1.4:67577175-67577194 MS.gene056082:CDS 40.0%
! GAGGTTGGTTATAGACTGAA+GGG - chr1.4:67577232-67577251 MS.gene056082:CDS 40.0%
ATTGCAACTGATACCTCAGC+TGG + chr1.4:67576995-67577014 None:intergenic 45.0%
GTTGGTTATAGACTGAAGGG+TGG - chr1.4:67577235-67577254 MS.gene056082:CDS 45.0%
! AGCTGAGGTATCAGTTGCAA+TGG - chr1.4:67576994-67577013 MS.gene056082:CDS 45.0%
! CACCCATCATCCCTTTTAGA+TGG + chr1.4:67577145-67577164 None:intergenic 45.0%
! GGAGGTTGGTTATAGACTGA+AGG - chr1.4:67577231-67577250 MS.gene056082:CDS 45.0%
!! CATCTAAAAGGGATGATGGG+TGG - chr1.4:67577143-67577162 MS.gene056082:CDS 45.0%
!! TAAAAGGGATGATGGGTGGA+TGG - chr1.4:67577147-67577166 MS.gene056082:CDS 45.0%
AAGGGTGGTCTCATCGTTGA+AGG - chr1.4:67577250-67577269 MS.gene056082:CDS 50.0%
CTGTGGTGAAGTTGACATGG+AGG - chr1.4:67577192-67577211 MS.gene056082:CDS 50.0%
ACTTGATTCTGCGCCAGCTG+AGG - chr1.4:67576979-67576998 MS.gene056082:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 67576971 67577294 67576971 ID=MS.gene056082
chr1.4 mRNA 67576971 67577294 67576971 ID=MS.gene056082.t1;Parent=MS.gene056082
chr1.4 exon 67576971 67577294 67576971 ID=MS.gene056082.t1.exon1;Parent=MS.gene056082.t1
chr1.4 CDS 67576971 67577294 67576971 ID=cds.MS.gene056082.t1;Parent=MS.gene056082.t1
Gene Sequence

>MS.gene056082

ATGTACGGACTTGATTCTGCGCCAGCTGAGGTATCAGTTGCAATGGCCAACAAAGTGCAAAATGGGATGGCATATTTGTACAATAAATACGAGGAGGATACTACGTTTTATGAAAAGCATAGAAAGATGGAAGGAAACAAATTGATAGAAGATAATAAGGATATTCGAGTTCCATCTAAAAGGGATGATGGGTGGATGGAAATTGAATTGGGTGAGTTTTTCTGTGGTGAAGTTGACATGGAGGTTAAGATTAGTGTTATGGAGGTTGGTTATAGACTGAAGGGTGGTCTCATCGTTGAAGGAGTTGAAGTTAGACCAAAATAA

Protein sequence

>MS.gene056082.t1

MYGLDSAPAEVSVAMANKVQNGMAYLYNKYEEDTTFYEKHRKMEGNKLIEDNKDIRVPSKRDDGWMEIELGEFFCGEVDMEVKISVMEVGYRLKGGLIVEGVEVRPK