Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066073.t1 | XP_013470213.1 | 98.9 | 89 | 1 | 0 | 1 | 89 | 230 | 318 | 5.40E-45 | 189.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066073.t1 | Q9LFR7 | 57.8 | 83 | 33 | 1 | 7 | 89 | 237 | 317 | 2.5e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066073.t1 | G7ZZC3 | 98.9 | 89 | 1 | 0 | 1 | 89 | 230 | 318 | 3.9e-45 | 189.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene066073 | 0.810554 | 1.09E-50 | -1.69E-46 |
| MS.gene049350 | MS.gene066073 | 0.814137 | 1.80E-51 | -1.69E-46 |
| MS.gene049585 | MS.gene066073 | 0.806858 | 6.71E-50 | -1.69E-46 |
| MS.gene049711 | MS.gene066073 | 0.808759 | 2.64E-50 | -1.69E-46 |
| MS.gene051332 | MS.gene066073 | 0.800415 | 1.46E-48 | -1.69E-46 |
| MS.gene052463 | MS.gene066073 | 0.822467 | 2.33E-53 | -1.69E-46 |
| MS.gene052534 | MS.gene066073 | 0.80029 | 1.55E-48 | -1.69E-46 |
| MS.gene05422 | MS.gene066073 | 0.804756 | 1.85E-49 | -1.69E-46 |
| MS.gene055313 | MS.gene066073 | 0.802855 | 4.60E-49 | -1.69E-46 |
| MS.gene059439 | MS.gene066073 | 0.822078 | 2.87E-53 | -1.69E-46 |
| MS.gene059965 | MS.gene066073 | 0.802607 | 5.18E-49 | -1.69E-46 |
| MS.gene061188 | MS.gene066073 | 0.826652 | 2.41E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066073.t1 | MTR_1g111280 | 97.778 | 90 | 2 | 0 | 1 | 90 | 230 | 319 | 1.03e-60 | 188 |
| MS.gene066073.t1 | MTR_7g406880 | 53.846 | 91 | 38 | 3 | 3 | 89 | 235 | 325 | 7.57e-28 | 103 |
| MS.gene066073.t1 | MTR_4g050140 | 42.353 | 85 | 43 | 2 | 9 | 89 | 212 | 294 | 6.76e-15 | 68.2 |
| MS.gene066073.t1 | MTR_7g107040 | 38.043 | 92 | 51 | 2 | 2 | 89 | 206 | 295 | 3.13e-12 | 60.8 |
| MS.gene066073.t1 | MTR_1g089310 | 43.056 | 72 | 39 | 2 | 9 | 78 | 238 | 309 | 7.69e-11 | 57.0 |
| MS.gene066073.t1 | MTR_1g089310 | 43.056 | 72 | 39 | 2 | 9 | 78 | 254 | 325 | 8.14e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066073.t1 | AT5G16080 | 57.831 | 83 | 33 | 1 | 7 | 89 | 237 | 317 | 3.02e-26 | 99.0 |
| MS.gene066073.t1 | AT1G68620 | 46.988 | 83 | 40 | 2 | 7 | 89 | 227 | 305 | 5.75e-18 | 76.6 |
| MS.gene066073.t1 | AT3G63010 | 44.615 | 65 | 34 | 2 | 15 | 77 | 245 | 309 | 9.46e-12 | 59.7 |
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGCAGCTTCAGATACTTAT+TGG | 0.192800 | 1.4:-85253307 | MS.gene066073:CDS |
| GTGTAATCCATTGGTGAAAA+TGG | 0.278139 | 1.4:-85253243 | MS.gene066073:CDS |
| GTTGAATGTGAAGTGTTTAA+AGG | 0.324790 | 1.4:-85253110 | MS.gene066073:CDS |
| TGGCGATTGGCTTTACCATA+TGG | 0.382531 | 1.4:-85253287 | MS.gene066073:CDS |
| AAATGCATGGAACAATTATC+TGG | 0.405311 | 1.4:-85253344 | None:intergenic |
| TGTGATGCTTTAGGAAGAAC+AGG | 0.432736 | 1.4:-85253140 | MS.gene066073:CDS |
| TTCAGATACTTATTGGCGAT+TGG | 0.462070 | 1.4:-85253300 | MS.gene066073:CDS |
| TGTGAAGTGTTTAAAGGTGT+TGG | 0.470256 | 1.4:-85253104 | MS.gene066073:CDS |
| CTTTAGGAAGAACAGGTACA+AGG | 0.492026 | 1.4:-85253133 | MS.gene066073:CDS |
| TGATCACGATCTTCACCATA+TGG | 0.493029 | 1.4:+85253272 | None:intergenic |
| ATCTGAGATGGATATATTGA+AGG | 0.500971 | 1.4:-85253180 | MS.gene066073:CDS |
| TATATTGAAGGATAGGAACT+TGG | 0.516422 | 1.4:-85253168 | MS.gene066073:CDS |
| AGATGGATATATTGAAGGAT+AGG | 0.521304 | 1.4:-85253175 | MS.gene066073:CDS |
| TCAGATATACACACCAACAT+AGG | 0.575792 | 1.4:+85253197 | None:intergenic |
| GCTACTGATGATGCCTATGT+TGG | 0.579488 | 1.4:-85253210 | MS.gene066073:CDS |
| TTCACCAATGGATTACACCA+TGG | 0.598695 | 1.4:+85253248 | None:intergenic |
| GTTGGTGTGTATATCTGAGA+TGG | 0.604384 | 1.4:-85253192 | MS.gene066073:CDS |
| TCATCCATGGTGTAATCCAT+TGG | 0.611826 | 1.4:-85253252 | MS.gene066073:CDS |
| TAATCCATTGGTGAAAATGG+AGG | 0.623717 | 1.4:-85253240 | MS.gene066073:CDS |
| GTGAAGATCGTGATCATCCA+TGG | 0.631742 | 1.4:-85253265 | MS.gene066073:CDS |
| TTTAGGAAGAACAGGTACAA+GGG | 0.650756 | 1.4:-85253132 | MS.gene066073:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AGATGGATATATTGAAGGAT+AGG | - | chr1.4:85253242-85253261 | MS.gene066073:CDS | 30.0% | |
| TATATTGAAGGATAGGAACT+TGG | - | chr1.4:85253249-85253268 | MS.gene066073:CDS | 30.0% | |
| ! | ATCTGAGATGGATATATTGA+AGG | - | chr1.4:85253237-85253256 | MS.gene066073:CDS | 30.0% |
| ! | GTTGAATGTGAAGTGTTTAA+AGG | - | chr1.4:85253307-85253326 | MS.gene066073:CDS | 30.0% |
| GTGTAATCCATTGGTGAAAA+TGG | - | chr1.4:85253174-85253193 | MS.gene066073:CDS | 35.0% | |
| TAATCCATTGGTGAAAATGG+AGG | - | chr1.4:85253177-85253196 | MS.gene066073:CDS | 35.0% | |
| TAGCAGCTTCAGATACTTAT+TGG | - | chr1.4:85253110-85253129 | MS.gene066073:CDS | 35.0% | |
| TCAGATATACACACCAACAT+AGG | + | chr1.4:85253223-85253242 | None:intergenic | 35.0% | |
| TTCAGATACTTATTGGCGAT+TGG | - | chr1.4:85253117-85253136 | MS.gene066073:CDS | 35.0% | |
| TTTAGGAAGAACAGGTACAA+GGG | - | chr1.4:85253285-85253304 | MS.gene066073:CDS | 35.0% | |
| ! | AATTCCTCCATTTTCACCAA+TGG | + | chr1.4:85253184-85253203 | None:intergenic | 35.0% |
| ! | TGTGAAGTGTTTAAAGGTGT+TGG | - | chr1.4:85253313-85253332 | MS.gene066073:CDS | 35.0% |
| !! | TTGGAGTTTTGTGATGCTTT+AGG | - | chr1.4:85253268-85253287 | MS.gene066073:CDS | 35.0% |
| GTTGGTGTGTATATCTGAGA+TGG | - | chr1.4:85253225-85253244 | MS.gene066073:CDS | 40.0% | |
| TCATCCATGGTGTAATCCAT+TGG | - | chr1.4:85253165-85253184 | MS.gene066073:CDS | 40.0% | |
| TGATCACGATCTTCACCATA+TGG | + | chr1.4:85253148-85253167 | None:intergenic | 40.0% | |
| ! | CTTTAGGAAGAACAGGTACA+AGG | - | chr1.4:85253284-85253303 | MS.gene066073:CDS | 40.0% |
| !! | TGTGATGCTTTAGGAAGAAC+AGG | - | chr1.4:85253277-85253296 | MS.gene066073:CDS | 40.0% |
| !! | TTCACCAATGGATTACACCA+TGG | + | chr1.4:85253172-85253191 | None:intergenic | 40.0% |
| GTGAAGATCGTGATCATCCA+TGG | - | chr1.4:85253152-85253171 | MS.gene066073:CDS | 45.0% | |
| ! | GCTACTGATGATGCCTATGT+TGG | - | chr1.4:85253207-85253226 | MS.gene066073:CDS | 45.0% |
| !! | TGGCGATTGGCTTTACCATA+TGG | - | chr1.4:85253130-85253149 | MS.gene066073:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 85253079 | 85253360 | 85253079 | ID=MS.gene066073 |
| chr1.4 | mRNA | 85253079 | 85253360 | 85253079 | ID=MS.gene066073.t1;Parent=MS.gene066073 |
| chr1.4 | exon | 85253079 | 85253360 | 85253079 | ID=MS.gene066073.t1.exon1;Parent=MS.gene066073.t1 |
| chr1.4 | CDS | 85253079 | 85253360 | 85253079 | ID=cds.MS.gene066073.t1;Parent=MS.gene066073.t1 |
| Gene Sequence |
| Protein sequence |
