AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066162

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066162.t1 MTR_2g069470 93.976 249 13 1 1 247 1 249 7.96e-173 476
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066162.t1 AT3G12930 61.728 243 79 5 2 235 1 238 6.96e-97 283

Find 70 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAGAGCTTTCTATAATT+TGG 0.135819 2.3:-26658954 MS.gene066162:CDS
CATACCCGATAGCATCAATT+TGG 0.241247 2.3:+26659602 None:intergenic
GTGAAAACCTTTGTGGGTTT+TGG 0.246984 2.3:+26661345 None:intergenic
TTGTGAAGCCACTTGTTTAC+TGG 0.250083 2.3:-26659663 MS.gene066162:CDS
TCTCTGCACTTGCAGGCTTT+TGG 0.287492 2.3:+26659971 None:intergenic
GATGAATTATTAAATAACTA+TGG 0.298468 2.3:-26660018 MS.gene066162:CDS
TTTCACCAGTTTCTGCAAAA+AGG 0.302040 2.3:-26661328 MS.gene066162:CDS
TCGTCAATCTCTGCACTTGC+AGG 0.326105 2.3:+26659964 None:intergenic
GGTCACCTTCTCCGGCGAAA+TGG 0.354058 2.3:-26661391 MS.gene066162:CDS
ATACCCGATAGCATCAATTT+GGG 0.374377 2.3:+26659603 None:intergenic
GTCACCTTCTCCGGCGAAAT+GGG 0.375268 2.3:-26661390 MS.gene066162:CDS
TTGTTATCAGCTTACATGAT+AGG 0.394608 2.3:+26659929 None:intergenic
AGCCAATGACAATTCTTTCT+CGG 0.397509 2.3:-26661229 MS.gene066162:intron
GACCGGGCTGAAAAGAAATA+TGG 0.400591 2.3:-26659461 MS.gene066162:CDS
ATGACTTACCGAAGTCCAAC+AGG 0.413851 2.3:+26659399 None:intergenic
GCATCTAGTTGCTGTGGAAC+TGG 0.415804 2.3:-26659731 MS.gene066162:intron
ATCGCTCCGGTCACCTTCTC+CGG 0.425960 2.3:-26661399 MS.gene066162:CDS
CACGGTTGCAAGTGAGGTTA+AGG 0.426301 2.3:-26659707 MS.gene066162:CDS
TCCAACAGGGTCCATGAGTT+GGG 0.438380 2.3:+26659413 None:intergenic
TACCGAGAAAGAATTGTCAT+TGG 0.438957 2.3:+26661227 None:intergenic
CGATGGCTCCGGCGAGGGAT+AGG 0.447540 2.3:+26661418 None:intergenic
AATTATAGAAAGCTCTTTGT+GGG 0.448717 2.3:+26658957 None:intergenic
AAACTGGTGAAAACCTTTGT+GGG 0.475786 2.3:+26661339 None:intergenic
CGTCCCCAAATTGATGCTAT+CGG 0.492807 2.3:-26659607 MS.gene066162:CDS
GTCCAACAGGGTCCATGAGT+TGG 0.493640 2.3:+26659412 None:intergenic
GTCCCCAAATTGATGCTATC+GGG 0.500874 2.3:-26659606 MS.gene066162:intron
TTTGGAGAAGAGTTGGTTTG+GGG 0.501179 2.3:+26661363 None:intergenic
GGTTAAGGCAGGGGATATAA+AGG 0.507409 2.3:-26659692 MS.gene066162:CDS
AAATATGGTAAAATTCCAAA+TGG 0.510572 2.3:-26659446 MS.gene066162:CDS
TAGGCTCCCTCATTGCGAAA+TGG 0.511514 2.3:+26658878 None:intergenic
AAATTATAGAAAGCTCTTTG+TGG 0.514327 2.3:+26658956 None:intergenic
ATGACCCATTTCGCCGGAGA+AGG 0.518592 2.3:+26661386 None:intergenic
ATGAATTATTAAATAACTAT+GGG 0.519880 2.3:-26660017 MS.gene066162:CDS
GAAACTGGTGAAAACCTTTG+TGG 0.521926 2.3:+26661338 None:intergenic
TCCACTCTCCTATCCCTCGC+CGG 0.530113 2.3:-26661426 MS.gene066162:CDS
CGGAGCGATGGCTCCGGCGA+GGG 0.533421 2.3:+26661413 None:intergenic
TTAGGTCGAGAATTAGAGAC+CGG 0.535827 2.3:-26659478 MS.gene066162:intron
ATTTCGCCGGAGAAGGTGAC+CGG 0.541431 2.3:+26661393 None:intergenic
GGTGACCGGAGCGATGGCTC+CGG 0.541587 2.3:+26661407 None:intergenic
GTTGCAAGTGAGGTTAAGGC+AGG 0.541952 2.3:-26659703 MS.gene066162:CDS
ACCCAACTCATGGACCCTGT+TGG 0.542471 2.3:-26659414 MS.gene066162:CDS
GCATCTTGAATTTGAAGAGA+AGG 0.544142 2.3:-26661284 MS.gene066162:CDS
TTTCGCAATGAGGGAGCCTA+CGG 0.547079 2.3:-26658875 MS.gene066162:CDS
AGTTGCTGTGGAACTGGCCA+CGG 0.547501 2.3:-26659725 MS.gene066162:CDS
CTCTTCTCCAAAACCCACAA+AGG 0.554228 2.3:-26661352 MS.gene066162:CDS
GAGACTCAAAACCCAACTCA+TGG 0.554759 2.3:-26659424 MS.gene066162:CDS
TGCAAGTGAGGTTAAGGCAG+GGG 0.558585 2.3:-26659701 MS.gene066162:CDS
CAACCACCATTTCGCAATGA+GGG 0.563409 2.3:-26658884 MS.gene066162:CDS
ACTGGCCACGGTTGCAAGTG+AGG 0.566137 2.3:-26659713 MS.gene066162:CDS
TCATGGACCCTGTTGGACTT+CGG 0.575861 2.3:-26659407 MS.gene066162:intron
AAGATGCTGTGGCGATGTAA+AGG 0.578301 2.3:+26661300 None:intergenic
TTGGGGATGACCCATTTCGC+CGG 0.581448 2.3:+26661380 None:intergenic
GGAGAAGGTGACCGGAGCGA+TGG 0.584683 2.3:+26661401 None:intergenic
CTTAACCTCACTTGCAACCG+TGG 0.590346 2.3:+26659708 None:intergenic
AATCGAGTCCAGTAAACAAG+TGG 0.595838 2.3:+26659655 None:intergenic
CCGGAGCGATGGCTCCGGCG+AGG 0.597203 2.3:+26661412 None:intergenic
TCTTCAAATTCAAGATGCTG+TGG 0.599629 2.3:+26661289 None:intergenic
GCTCCCTCATTGCGAAATGG+TGG 0.600231 2.3:+26658881 None:intergenic
CCTCGCCGGAGCCATCGCTC+CGG 0.600870 2.3:-26661412 MS.gene066162:CDS
TCAACCACCATTTCGCAATG+AGG 0.606311 2.3:-26658885 MS.gene066162:CDS
TTATAGAAAGCTCTTTGTGG+GGG 0.611682 2.3:+26658959 None:intergenic
TTGCAAGTGAGGTTAAGGCA+GGG 0.616058 2.3:-26659702 MS.gene066162:CDS
TGTTGTGCATCTAGTTGCTG+TGG 0.619390 2.3:-26659737 MS.gene066162:intron
ATTATAGAAAGCTCTTTGTG+GGG 0.619735 2.3:+26658958 None:intergenic
AGGGATAGGAGAGTGGAAGA+CGG 0.644410 2.3:+26661432 None:intergenic
TACCCGATAGCATCAATTTG+GGG 0.655032 2.3:+26659604 None:intergenic
TCCGGCGAGGGATAGGAGAG+TGG 0.665115 2.3:+26661425 None:intergenic
TAGGTCGAGAATTAGAGACC+GGG 0.675536 2.3:-26659477 MS.gene066162:CDS
TGCATCATCTGAGAAACCGT+AGG 0.677693 2.3:+26658859 None:intergenic
TGACTTACCGAAGTCCAACA+GGG 0.707635 2.3:+26659400 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTATTAAATAACTAT+GGG - chr2.3:26660271-26660290 MS.gene066162:intron 10.0%
!! TTTAATGTTTATACATTATT+TGG - chr2.3:26659564-26659583 MS.gene066162:intron 10.0%
!! ATGAATTTATATAATCACAA+TGG - chr2.3:26660110-26660129 MS.gene066162:intron 15.0%
!! GATGAATTATTAAATAACTA+TGG - chr2.3:26660270-26660289 MS.gene066162:intron 15.0%
!!! TACTTTTAAGTTATTATTAC+CGG - chr2.3:26659343-26659362 MS.gene066162:intron 15.0%
!! AAATATGGTAAAATTCCAAA+TGG - chr2.3:26660842-26660861 MS.gene066162:intron 20.0%
!! AATATATAAAGATCAATGAC+AGG + chr2.3:26660715-26660734 None:intergenic 20.0%
!! GAAATATGAATACATAACAA+TGG + chr2.3:26659476-26659495 None:intergenic 20.0%
!! TAAAAGAAAAACAACTACAT+AGG + chr2.3:26659447-26659466 None:intergenic 20.0%
!! TAAGAATCAACATTAAACAA+AGG + chr2.3:26660002-26660021 None:intergenic 20.0%
!! TGTAAAAAGAAATGTAAACA+AGG + chr2.3:26660409-26660428 None:intergenic 20.0%
!!! TAACAAACTGTATAATTTTC+AGG - chr2.3:26660218-26660237 MS.gene066162:intron 20.0%
!!! TATAATTTGGAAGAGTTTTA+TGG - chr2.3:26661347-26661366 MS.gene066162:CDS 20.0%
!!! TTTATGTTGATTGATTTAGA+AGG + chr2.3:26661120-26661139 None:intergenic 20.0%
!!! TTTTTTTTTTCTTTGCTCAA+AGG - chr2.3:26660753-26660772 MS.gene066162:intron 20.0%
! AAATTATAGAAAGCTCTTTG+TGG + chr2.3:26661335-26661354 None:intergenic 25.0%
! AATTATAGAAAGCTCTTTGT+GGG + chr2.3:26661334-26661353 None:intergenic 25.0%
! ACAAAGAGCTTTCTATAATT+TGG - chr2.3:26661334-26661353 MS.gene066162:CDS 25.0%
! ATAAAATGCAGACAATTAGA+GGG + chr2.3:26660932-26660951 None:intergenic 25.0%
! CAACTATTCTATAAGATTGA+AGG - chr2.3:26659241-26659260 MS.gene066162:intron 25.0%
! CTTCTGATTATGTCAAATAT+AGG + chr2.3:26661168-26661187 None:intergenic 25.0%
! GATTCAGAATAAGATGTTTA+AGG - chr2.3:26659510-26659529 MS.gene066162:intron 25.0%
! GTAAAATGTTAACTGAGATA+AGG - chr2.3:26659616-26659635 MS.gene066162:CDS 25.0%
! TATAAAATGCAGACAATTAG+AGG + chr2.3:26660933-26660952 None:intergenic 25.0%
! TATTGCAACATAAATTGCAT+TGG - chr2.3:26661190-26661209 MS.gene066162:intron 25.0%
!! AATGAGTTCAGTTTAGATTT+TGG - chr2.3:26659136-26659155 MS.gene066162:intron 25.0%
!! ATACTTTTGAACTTTGCTTT+CGG - chr2.3:26659030-26659049 MS.gene066162:intron 25.0%
!! ATGAGTTCAGTTTAGATTTT+GGG - chr2.3:26659137-26659156 MS.gene066162:intron 25.0%
!! TTATGTCAAATATAGGCTTT+TGG + chr2.3:26661161-26661180 None:intergenic 25.0%
!!! AGTTTTGGTAGTTAAAATTC+AGG + chr2.3:26660029-26660048 None:intergenic 25.0%
!!! TGATTTAGAAGGTTTTGATA+GGG + chr2.3:26661109-26661128 None:intergenic 25.0%
!!! TTGATTTAGAAGGTTTTGAT+AGG + chr2.3:26661110-26661129 None:intergenic 25.0%
AAACAATCATAAGATGAACC+TGG + chr2.3:26661272-26661291 None:intergenic 30.0%
AAATAACCGAACCATAGTAA+AGG + chr2.3:26659658-26659677 None:intergenic 30.0%
AATGTTAACTGAGATAAGGT+TGG - chr2.3:26659620-26659639 MS.gene066162:CDS 30.0%
ACATGTATGTGTCTTATATC+AGG - chr2.3:26660441-26660460 MS.gene066162:intron 30.0%
ATGCAATTTAGAAAACCTTG+AGG - chr2.3:26659215-26659234 MS.gene066162:intron 30.0%
ATTATAGAAAGCTCTTTGTG+GGG + chr2.3:26661333-26661352 None:intergenic 30.0%
GACTAAACCAAATACGTAAT+AGG + chr2.3:26660069-26660088 None:intergenic 30.0%
TAACACCTAAACATAAACAC+CGG + chr2.3:26659365-26659384 None:intergenic 30.0%
TACCAAACAAGATACAAGAT+AGG + chr2.3:26661224-26661243 None:intergenic 30.0%
TATTACCGGTGTTTATGTTT+AGG - chr2.3:26659357-26659376 MS.gene066162:intron 30.0%
TGATTGGCAATTCTCATTAA+GGG - chr2.3:26660187-26660206 MS.gene066162:intron 30.0%
TTAACTACCAAAACTGAGAA+TGG - chr2.3:26660034-26660053 MS.gene066162:CDS 30.0%
TTAATGAGAATTGCCAATCA+CGG + chr2.3:26660187-26660206 None:intergenic 30.0%
TTAGGTGTTAATGTTGTGTT+CGG - chr2.3:26659375-26659394 MS.gene066162:intron 30.0%
TTGTTATCAGCTTACATGAT+AGG + chr2.3:26660362-26660381 None:intergenic 30.0%
! ACAATTTCTAGACACTAAAG+AGG + chr2.3:26659954-26659973 None:intergenic 30.0%
! ATAGTCCTATCTTTTAAACG+TGG + chr2.3:26659759-26659778 None:intergenic 30.0%
! TAGTCCTATCTTTTAAACGT+GGG + chr2.3:26659758-26659777 None:intergenic 30.0%
! TATGATTGTTTCTTGATCAC+AGG - chr2.3:26661281-26661300 MS.gene066162:CDS 30.0%
! TATTTGGATGTTGACTATAG+AGG - chr2.3:26659580-26659599 MS.gene066162:intron 30.0%
! TCTAGTTCCTATTACGTATT+TGG - chr2.3:26660059-26660078 MS.gene066162:intron 30.0%
!! AATGGTGGTTGATTTTCAAA+AGG + chr2.3:26661395-26661414 None:intergenic 30.0%
!! GTTTTATGGTAATGCAACAT+CGG - chr2.3:26661361-26661380 MS.gene066162:CDS 30.0%
!! TACTTTAAAGCGCTCAAATT+GGG + chr2.3:26659704-26659723 None:intergenic 30.0%
!!! AGTTTAGATTTTGGGTTCTA+TGG - chr2.3:26659145-26659164 MS.gene066162:intron 30.0%
!!! CAAATATAGGCTTTTGGAAT+TGG + chr2.3:26661155-26661174 None:intergenic 30.0%
!!! GGGTTTGGTTTTTAAAACAT+TGG - chr2.3:26659890-26659909 MS.gene066162:intron 30.0%
!!! TTACTATGGTTCGGTTATTT+TGG - chr2.3:26659658-26659677 MS.gene066162:CDS 30.0%
AAACTGGTGAAAACCTTTGT+GGG + chr2.3:26658952-26658971 None:intergenic 35.0%
AAGAGGTTAGAATCATGTTG+AGG + chr2.3:26661083-26661102 None:intergenic 35.0%
AAGTCCCACGTTTAAAAGAT+AGG - chr2.3:26659751-26659770 MS.gene066162:intron 35.0%
AGCCAATGACAATTCTTTCT+CGG - chr2.3:26659059-26659078 MS.gene066162:intron 35.0%
ATAATCAGAAGCAAAGATGC+AGG - chr2.3:26661054-26661073 MS.gene066162:intron 35.0%
ATACCCGATAGCATCAATTT+GGG + chr2.3:26660688-26660707 None:intergenic 35.0%
ATAGAATAGTTGTCTCCTCA+AGG + chr2.3:26659233-26659252 None:intergenic 35.0%
GCATCTTGAATTTGAAGAGA+AGG - chr2.3:26659004-26659023 MS.gene066162:intron 35.0%
GTCCTATCTTGTATCTTGTT+TGG - chr2.3:26661219-26661238 MS.gene066162:intron 35.0%
GTGATTGGCAATTCTCATTA+AGG - chr2.3:26660186-26660205 MS.gene066162:intron 35.0%
TACCGAGAAAGAATTGTCAT+TGG + chr2.3:26659064-26659083 None:intergenic 35.0%
TCAGACTGTATTCAGAAACA+CGG + chr2.3:26661248-26661267 None:intergenic 35.0%
TCTAGAAGTTATGTCTTTGC+TGG + chr2.3:26661006-26661025 None:intergenic 35.0%
TCTTCAAATTCAAGATGCTG+TGG + chr2.3:26659002-26659021 None:intergenic 35.0%
TTATAGAAAGCTCTTTGTGG+GGG + chr2.3:26661332-26661351 None:intergenic 35.0%
TTTCACCAGTTTCTGCAAAA+AGG - chr2.3:26658960-26658979 MS.gene066162:CDS 35.0%
TTTGTGGGGGAAGAAATAAA+TGG + chr2.3:26661319-26661338 None:intergenic 35.0%
! ATCTTTTAAACGTGGGACTT+GGG + chr2.3:26659751-26659770 None:intergenic 35.0%
! CTAGATTCCATTCTCAGTTT+TGG + chr2.3:26660044-26660063 None:intergenic 35.0%
! TACCATATTTCTTTTCAGCC+CGG + chr2.3:26660832-26660851 None:intergenic 35.0%
! TATCTTTTAAACGTGGGACT+TGG + chr2.3:26659752-26659771 None:intergenic 35.0%
!! CTACTTTAAAGCGCTCAAAT+TGG + chr2.3:26659705-26659724 None:intergenic 35.0%
!! TTTTGGAGAAGAGTTGGTTT+GGG + chr2.3:26658929-26658948 None:intergenic 35.0%
AAGGGGAAAAAGTTGTGGTT+TGG + chr2.3:26659926-26659945 None:intergenic 40.0%
AATCGAGTCCAGTAAACAAG+TGG + chr2.3:26660636-26660655 None:intergenic 40.0%
ACACTAAAGAGGAGATGAAG+GGG + chr2.3:26659943-26659962 None:intergenic 40.0%
ACATTCAACACCTTACAAGC+TGG - chr2.3:26659801-26659820 MS.gene066162:intron 40.0%
AGACACTAAAGAGGAGATGA+AGG + chr2.3:26659945-26659964 None:intergenic 40.0%
AGATGAAGGGGAAAAAGTTG+TGG + chr2.3:26659931-26659950 None:intergenic 40.0%
AGGGGAAAAAGTTGTGGTTT+GGG + chr2.3:26659925-26659944 None:intergenic 40.0%
CAAATTGATGCTATCGGGTA+TGG - chr2.3:26660687-26660706 MS.gene066162:intron 40.0%
CATACCCGATAGCATCAATT+TGG + chr2.3:26660689-26660708 None:intergenic 40.0%
CCTTACAAAACCAGCTTGTA+AGG + chr2.3:26659814-26659833 None:intergenic 40.0%
GAAACTGGTGAAAACCTTTG+TGG + chr2.3:26658953-26658972 None:intergenic 40.0%
GACACTAAAGAGGAGATGAA+GGG + chr2.3:26659944-26659963 None:intergenic 40.0%
TACCCGATAGCATCAATTTG+GGG + chr2.3:26660687-26660706 None:intergenic 40.0%
TGACACTGACACTGATAACT+TGG - chr2.3:26659309-26659328 MS.gene066162:intron 40.0%
TTAGGTCGAGAATTAGAGAC+CGG - chr2.3:26660810-26660829 MS.gene066162:intron 40.0%
TTCTGAATACAGTCTGATCC+AGG - chr2.3:26661251-26661270 MS.gene066162:CDS 40.0%
TTGTGAAGCCACTTGTTTAC+TGG - chr2.3:26660625-26660644 MS.gene066162:intron 40.0%
! GTGAAAACCTTTGTGGGTTT+TGG + chr2.3:26658946-26658965 None:intergenic 40.0%
!! AGGTTTTGATAGGGACAAAG+AGG + chr2.3:26661100-26661119 None:intergenic 40.0%
!! TTTGGAGAAGAGTTGGTTTG+GGG + chr2.3:26658928-26658947 None:intergenic 40.0%
!!! CCTTACAAGCTGGTTTTGTA+AGG - chr2.3:26659811-26659830 MS.gene066162:intron 40.0%
!!! GTTTTGGAGAAGAGTTGGTT+TGG + chr2.3:26658930-26658949 None:intergenic 40.0%
!!! TTGGGTTTTGAGTCTCCATT+TGG + chr2.3:26660860-26660879 None:intergenic 40.0%
AAGATGCTGTGGCGATGTAA+AGG + chr2.3:26658991-26659010 None:intergenic 45.0%
ACCATAGTAAAGGCCACGAT+CGG + chr2.3:26659648-26659667 None:intergenic 45.0%
AGTGCTTCATAGTTCACAGG+AGG - chr2.3:26659412-26659431 MS.gene066162:CDS 45.0%
ATCTCTCACTGGTGTGTTGT+CGG - chr2.3:26659869-26659888 MS.gene066162:intron 45.0%
ATGACTTACCGAAGTCCAAC+AGG + chr2.3:26660892-26660911 None:intergenic 45.0%
CAACCACCATTTCGCAATGA+GGG - chr2.3:26661404-26661423 MS.gene066162:CDS 45.0%
CGTCCCCAAATTGATGCTAT+CGG - chr2.3:26660681-26660700 MS.gene066162:intron 45.0%
CTCTTCTCCAAAACCCACAA+AGG - chr2.3:26658936-26658955 MS.gene066162:CDS 45.0%
GACCGGGCTGAAAAGAAATA+TGG - chr2.3:26660827-26660846 MS.gene066162:intron 45.0%
GAGACTCAAAACCCAACTCA+TGG - chr2.3:26660864-26660883 MS.gene066162:intron 45.0%
GCTAGTGCTTCATAGTTCAC+AGG - chr2.3:26659409-26659428 MS.gene066162:CDS 45.0%
GGTTAAGGCAGGGGATATAA+AGG - chr2.3:26660596-26660615 MS.gene066162:intron 45.0%
GTCCCCAAATTGATGCTATC+GGG - chr2.3:26660682-26660701 MS.gene066162:intron 45.0%
TAGGTCGAGAATTAGAGACC+GGG - chr2.3:26660811-26660830 MS.gene066162:intron 45.0%
TCAACCACCATTTCGCAATG+AGG - chr2.3:26661403-26661422 MS.gene066162:CDS 45.0%
TCGTGGCCTTTACTATGGTT+CGG - chr2.3:26659649-26659668 MS.gene066162:CDS 45.0%
TGACTTACCGAAGTCCAACA+GGG + chr2.3:26660891-26660910 None:intergenic 45.0%
TGCATCATCTGAGAAACCGT+AGG + chr2.3:26661432-26661451 None:intergenic 45.0%
TGTTGTGCATCTAGTTGCTG+TGG - chr2.3:26660551-26660570 MS.gene066162:intron 45.0%
TTGCAAGTGAGGTTAAGGCA+GGG - chr2.3:26660586-26660605 MS.gene066162:intron 45.0%
! ATGCGCCTTTTTGCAGAAAC+TGG + chr2.3:26658968-26658987 None:intergenic 45.0%
! GCCTCTTCTAGAAGTGCAAT+GGG + chr2.3:26660976-26660995 None:intergenic 45.0%
! TCCCATTGCACTTCTAGAAG+AGG - chr2.3:26660972-26660991 MS.gene066162:intron 45.0%
! TGCCTCTTCTAGAAGTGCAA+TGG + chr2.3:26660977-26660996 None:intergenic 45.0%
! TGGAGCGTAGCGTTGAAATT+GGG + chr2.3:26659848-26659867 None:intergenic 45.0%
!!! ACGTGGGACTTGGGTTTTTT+CGG + chr2.3:26659742-26659761 None:intergenic 45.0%
!!! TGTGGGTTTTGGAGAAGAGT+TGG + chr2.3:26658935-26658954 None:intergenic 45.0%
!! AAATAAAATTCAATATAAAT+AGG + chr2.3:26660792-26660811 None:intergenic 5.0%
!!! ATTTATATTGAATTTTATTT+AGG - chr2.3:26660792-26660811 MS.gene066162:intron 5.0%
AGGGATAGGAGAGTGGAAGA+CGG + chr2.3:26658859-26658878 None:intergenic 50.0%
CAACACACCAGTGAGAGATC+TGG + chr2.3:26659868-26659887 None:intergenic 50.0%
CACGGTTGCAAGTGAGGTTA+AGG - chr2.3:26660581-26660600 MS.gene066162:intron 50.0%
CTTAACCTCACTTGCAACCG+TGG + chr2.3:26660583-26660602 None:intergenic 50.0%
GCATCTAGTTGCTGTGGAAC+TGG - chr2.3:26660557-26660576 MS.gene066162:intron 50.0%
GTTGCAAGTGAGGTTAAGGC+AGG - chr2.3:26660585-26660604 MS.gene066162:intron 50.0%
TAGGCTCCCTCATTGCGAAA+TGG + chr2.3:26661413-26661432 None:intergenic 50.0%
TCATGGACCCTGTTGGACTT+CGG - chr2.3:26660881-26660900 MS.gene066162:intron 50.0%
TCCAACAGGGTCCATGAGTT+GGG + chr2.3:26660878-26660897 None:intergenic 50.0%
TCCGATCGTGGCCTTTACTA+TGG - chr2.3:26659644-26659663 MS.gene066162:CDS 50.0%
TCGTCAATCTCTGCACTTGC+AGG + chr2.3:26660327-26660346 None:intergenic 50.0%
TCTCTCACTGGTGTGTTGTC+GGG - chr2.3:26659870-26659889 MS.gene066162:intron 50.0%
TGCAAGTGAGGTTAAGGCAG+GGG - chr2.3:26660587-26660606 MS.gene066162:intron 50.0%
TTTCGCAATGAGGGAGCCTA+CGG - chr2.3:26661413-26661432 MS.gene066162:CDS 50.0%
! CTGGAGCGTAGCGTTGAAAT+TGG + chr2.3:26659849-26659868 None:intergenic 50.0%
! GATAAGGTTGGTTCCGATCG+TGG - chr2.3:26659632-26659651 MS.gene066162:CDS 50.0%
! TCTCTGCACTTGCAGGCTTT+TGG + chr2.3:26660320-26660339 None:intergenic 50.0%
!!! CGTGGGACTTGGGTTTTTTC+GGG + chr2.3:26659741-26659760 None:intergenic 50.0%
ACCCAACTCATGGACCCTGT+TGG - chr2.3:26660874-26660893 MS.gene066162:intron 55.0%
AGTTGCTGTGGAACTGGCCA+CGG - chr2.3:26660563-26660582 MS.gene066162:intron 55.0%
ATGACCCATTTCGCCGGAGA+AGG + chr2.3:26658905-26658924 None:intergenic 55.0%
ATTGCCAATCACGGAACGCG+TGG + chr2.3:26660178-26660197 None:intergenic 55.0%
ATTTCGCCGGAGAAGGTGAC+CGG + chr2.3:26658898-26658917 None:intergenic 55.0%
CACTGGTGTGTTGTCGGGTT+TGG - chr2.3:26659875-26659894 MS.gene066162:intron 55.0%
CTACGCTCCAGATCTCTCAC+TGG - chr2.3:26659858-26659877 MS.gene066162:intron 55.0%
GCTCCCTCATTGCGAAATGG+TGG + chr2.3:26661410-26661429 None:intergenic 55.0%
GTCACCTTCTCCGGCGAAAT+GGG - chr2.3:26658898-26658917 MS.gene066162:CDS 55.0%
GTCCAACAGGGTCCATGAGT+TGG + chr2.3:26660879-26660898 None:intergenic 55.0%
TTGGGGATGACCCATTTCGC+CGG + chr2.3:26658911-26658930 None:intergenic 55.0%
ACTGGCCACGGTTGCAAGTG+AGG - chr2.3:26660575-26660594 MS.gene066162:intron 60.0%
ATCGCTCCGGTCACCTTCTC+CGG - chr2.3:26658889-26658908 MS.gene066162:CDS 60.0%
CGCGTGGCAGGTAGAAAAGC+AGG + chr2.3:26660162-26660181 None:intergenic 60.0%
GGTCACCTTCTCCGGCGAAA+TGG - chr2.3:26658897-26658916 MS.gene066162:CDS 60.0%
TCCACTCTCCTATCCCTCGC+CGG - chr2.3:26658862-26658881 MS.gene066162:CDS 60.0%
CCAATCACGGAACGCGTGGC+AGG + chr2.3:26660174-26660193 None:intergenic 65.0%
CCTGCCACGCGTTCCGTGAT+TGG - chr2.3:26660171-26660190 MS.gene066162:intron 65.0%
GGAGAAGGTGACCGGAGCGA+TGG + chr2.3:26658890-26658909 None:intergenic 65.0%
TCCGGCGAGGGATAGGAGAG+TGG + chr2.3:26658866-26658885 None:intergenic 65.0%
CGATGGCTCCGGCGAGGGAT+AGG + chr2.3:26658873-26658892 None:intergenic 70.0%
GGTGACCGGAGCGATGGCTC+CGG + chr2.3:26658884-26658903 None:intergenic 70.0%
CCTCGCCGGAGCCATCGCTC+CGG - chr2.3:26658876-26658895 MS.gene066162:CDS 75.0%
CGGAGCGATGGCTCCGGCGA+GGG + chr2.3:26658878-26658897 None:intergenic 75.0%
! CCGGAGCGATGGCTCCGGCG+AGG + chr2.3:26658879-26658898 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 26658850 26661460 26658850 ID=MS.gene066162
chr2.3 mRNA 26658850 26661460 26658850 ID=MS.gene066162.t1;Parent=MS.gene066162
chr2.3 exon 26661230 26661460 26661230 ID=MS.gene066162.t1.exon1;Parent=MS.gene066162.t1
chr2.3 CDS 26661230 26661460 26661230 ID=cds.MS.gene066162.t1;Parent=MS.gene066162.t1
chr2.3 exon 26659944 26660070 26659944 ID=MS.gene066162.t1.exon2;Parent=MS.gene066162.t1
chr2.3 CDS 26659944 26660070 26659944 ID=cds.MS.gene066162.t1;Parent=MS.gene066162.t1
chr2.3 exon 26659607 26659745 26659607 ID=MS.gene066162.t1.exon3;Parent=MS.gene066162.t1
chr2.3 CDS 26659607 26659745 26659607 ID=cds.MS.gene066162.t1;Parent=MS.gene066162.t1
chr2.3 exon 26659408 26659496 26659408 ID=MS.gene066162.t1.exon4;Parent=MS.gene066162.t1
chr2.3 CDS 26659408 26659496 26659408 ID=cds.MS.gene066162.t1;Parent=MS.gene066162.t1
chr2.3 exon 26658850 26659007 26658850 ID=MS.gene066162.t1.exon5;Parent=MS.gene066162.t1
chr2.3 CDS 26658850 26659007 26658850 ID=cds.MS.gene066162.t1;Parent=MS.gene066162.t1
Gene Sequence

>MS.gene066162

ATGATACCGTCTTCCACTCTCCTATCCCTCGCCGGAGCCATCGCTCCGGTCACCTTCTCCGGCGAAATGGGTCATCCCCAAACCAACTCTTCTCCAAAACCCACAAAGGTTTTCACCAGTTTCTGCAAAAAGGCGCATCCTTTACATCGCCACAGCATCTTGAATTTGAAGAGAAGGAGCATACTTTTGAACTTTGCTTTCGGTAAAGAAGCCAATGACAATTCTTTCTCGGTATAATACTTTCATATTGCTGCGACAGTTATTTATTTTATTTTATTTTTTCAAGAATGAGTTCAGTTTAGATTTTGGGTTCTATGGAAAGTGAAATATTTTGAAAGCATGCTTAAATGTAAAATATGTTCATTATGCAATTTAGAAAACCTTGAGGAGACAACTATTCTATAAGATTGAAGGTGTGTCTAGTGTACGACACAACACAACTTGTGTGTTATGATTGTGTGACACTGACACTGATAACTTGGTTATATTCAATTACTTTTAAGTTATTATTACCGGTGTTTATGTTTAGGTGTTAATGTTGTGTTCGGTGTGTGTCTGTGCTAGTGCTTCATAGTTCACAGGAGGAACTCTTTTCCTATGTAGTTGTTTTTCTTTTATGAAATCCATTGTTATGTATTCATATTTCATCAGATGTATATAGATTCAGAATAAGATGTTTAAGGCATTTTGCTTAAGTACATGTCTAAAGACAATTTTAATGTTTATACATTATTTGGATGTTGACTATAGAGGATTTTAACATAATGTAAAATGTTAACTGAGATAAGGTTGGTTCCGATCGTGGCCTTTACTATGGTTCGGTTATTTTGGCAGTTTGATCAGTGTTTTGTCCCAATTTGAGCGCTTTAAAGTAGTTACGCTATTTATCCCGAAAAAACCCAAGTCCCACGTTTAAAAGATAGGACTATAGATAAGAGTTTATAAAGAGAGACATTCAACACCTTACAAGCTGGTTTTGTAAGGATTGAGTTAGACCCAATTTCAACGCTACGCTCCAGATCTCTCACTGGTGTGTTGTCGGGTTTGGTTTTTAAAACATTGGTTTTAAACTCCCAAACCACAACTTTTTCCCCTTCATCTCCTCTTTAGTGTCTAGAAATTGTCATTCGTTTTTCATCGCTCTCTTTTCCTTTGTTTAATGTTGATTCTTAGAGACCTGAATTTTAACTACCAAAACTGAGAATGGAATCTAGTTCCTATTACGTATTTGGTTTAGTCTTTTTTTTATCTGTGCGTTGAATGAATTTATATAATCACAATGGTTCGATCTTGTGTAGAATTTCAACTGCCTGCTTTTCTACCTGCCACGCGTTCCGTGATTGGCAATTCTCATTAAGGGTACATAAGTAACAAACTGTATAATTTTCAGGATGTAGACGAAGATACTGATGATATGTATGATGAATTATTAAATAACTATGGGCAAGTAGTATATAAGAGAAAAGACCAAAAGCCTGCAAGTGCAGAGATTGACGATGATTCTGAGAGCCTATCATGTAAGCTGATAACAAGTTTTGAAAATTAATTTGCTTCTACCTTGTTTACATTTCTTTTTACAATCTCTTTTTAAACATGTATGTGTCTTATATCAGGTTTCTTTGTAATTGTATTTGTTCAACTGTTATAAAAACTTGTTCAGTTTATGTCTATATGAATCTATCTAATGTTGAGTATGTTAACTGTTGTGCATCTAGTTGCTGTGGAACTGGCCACGGTTGCAAGTGAGGTTAAGGCAGGGGATATAAAGGTCTTGTTTGTGAAGCCACTTGTTTACTGGACTCGATTTTTTATCATAGCTACAGCATTTTCTCGTCCCCAAATTGATGCTATCGGGTATGGAACCTGTCATTGATCTTTATATATTTATTTAGCAGTGCTCAATTTTTTTTTTTCTTTGCTCAAAGGATGTATATTTAATCCTATTTATATTGAATTTTATTTAGGTCGAGAATTAGAGACCGGGCTGAAAAGAAATATGGTAAAATTCCAAATGGAGACTCAAAACCCAACTCATGGACCCTGTTGGACTTCGGTAAGTCATTTTACAATACAATAGAGCCCTCTAATTGTCTGCATTTTATAAAAAAAAATCATTGAAGTTTCCCATTGCACTTCTAGAAGAGGCACATTCACCAGCAAAGACATAACTTCTAGAAATTTAACATTATACTCTAACAAAATGCATAATCAGAAGCAAAGATGCAGGATACCTCAACATGATTCTAACCTCTTTGTCCCTATCAAAACCTTCTAAATCAATCAACATAAATTGCATCAAATTCCAATTCCAAAAGCCTATATTTGACATAATCAGAAGTCTATTGCAACATAAATTGCATTGGATCGTAGTCCTATCTTGTATCTTGTTTGGTATCCGTGTTTCTGAATACAGTCTGATCCAGGTTCATCTTATGATTGTTTCTTGATCACAGGTGATGTTGTTGTCCATTTATTTCTTCCCCCACAAAGAGCTTTCTATAATTTGGAAGAGTTTTATGGTAATGCAACATCGGTAGAGCTGCCTTTTGAAAATCAACCACCATTTCGCAATGAGGGAGCCTACGGTTTCTCAGATGATGCAGACAGTTGA
Protein sequence

>MS.gene066162.t1

MIPSSTLLSLAGAIAPVTFSGEMGHPQTNSSPKPTKVFTSFCKKAHPLHRHSILNLKRRSILLNFAFGKEANDNSFSDVDEDTDDMYDELLNNYGQVVYKRKDQKPASAEIDDDSESLSFAVELATVASEVKAGDIKVLFVKPLVYWTRFFIIATAFSRPQIDAIGSRIRDRAEKKYGKIPNGDSKPNSWTLLDFGDVVVHLFLPPQRAFYNLEEFYGNATSVELPFENQPPFRNEGAYGFSDDADS