AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066243


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066243.t1 MTR_1g099320 96.633 297 10 0 1 297 1 297 0.0 566
MS.gene066243.t1 MTR_1g099350 70.805 298 79 4 1 297 1 291 5.10e-149 419
MS.gene066243.t1 MTR_0027s0260 67.708 288 87 4 7 293 12 294 1.45e-139 395
MS.gene066243.t1 MTR_8g055940 68.440 282 84 3 13 293 16 293 6.07e-137 389
MS.gene066243.t1 MTR_1g099310 65.188 293 96 6 7 297 8 296 8.79e-135 384
MS.gene066243.t1 MTR_5g043550 63.946 294 101 3 1 293 1 290 8.11e-133 379
MS.gene066243.t1 MTR_7g116350 61.986 292 95 2 7 297 8 284 2.20e-124 374
MS.gene066243.t1 MTR_1g099290 63.158 285 100 5 13 295 15 296 3.66e-124 367
MS.gene066243.t1 MTR_8g467650 62.284 289 105 3 7 293 8 294 2.93e-123 354
MS.gene066243.t1 MTR_2g102020 57.667 300 116 5 8 297 15 313 2.14e-120 349
MS.gene066243.t1 MTR_7g116850 56.701 291 121 4 11 297 37 326 8.42e-114 332
MS.gene066243.t1 MTR_2g040740 55.634 284 118 4 14 293 16 295 7.48e-105 309
MS.gene066243.t1 MTR_2g040830 53.684 285 123 5 14 293 16 296 1.53e-98 293
MS.gene066243.t1 MTR_2g040690 52.982 285 125 5 14 293 18 298 4.60e-98 291
MS.gene066243.t1 MTR_6g023340 50.000 286 134 7 18 297 20 302 3.16e-93 278
MS.gene066243.t1 MTR_2g040650 48.421 285 134 6 14 293 16 292 2.45e-86 261
MS.gene066243.t1 MTR_2g040870 49.057 159 77 2 16 174 15 169 3.22e-41 140
MS.gene066243.t1 MTR_2g040880 52.212 113 49 3 186 293 1 113 1.62e-35 125
MS.gene066243.t1 MTR_2g040890 42.683 164 83 6 9 166 8 166 5.20e-35 125
MS.gene066243.t1 MTR_2g040850 45.033 151 73 3 2 145 3 150 2.35e-31 117
MS.gene066243.t1 MTR_2g040900 47.500 120 57 4 180 293 18 137 4.60e-28 106
MS.gene066243.t1 MTR_4g077310 53.933 89 37 4 207 293 3 89 2.17e-23 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066243.t1 AT5G24090 64.000 300 104 3 1 297 4 302 1.68e-143 406

Find 59 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTTGATTCCTTTATTT+TGG 0.131017 1.1:-71504510 None:intergenic
TTGTGAATTCATGGAATAAA+TGG 0.242224 1.1:+71504899 MS.gene066243:CDS
AACTTGGTTCCACCGAAAAT+TGG 0.244758 1.1:+71504689 MS.gene066243:CDS
CTTTCAATTGGTGGTGGAAT+TGG 0.287500 1.1:+71504535 MS.gene066243:CDS
AGGTTCCTTCGTTGCCATTT+TGG 0.290608 1.1:-71504330 None:intergenic
GCAATCTATTGGGGCCAAAA+TGG 0.300822 1.1:+71504316 MS.gene066243:CDS
GGGTATAGCACTTCTATTAT+TGG 0.304491 1.1:+71505096 MS.gene066243:CDS
TGACCTGCGAGATTCATTTC+AGG 0.305610 1.1:-71504430 None:intergenic
TAACTAACCTTGTGAATTCA+TGG 0.311751 1.1:+71504890 MS.gene066243:CDS
AATCCACCTTGTCAATATAA+TGG 0.323098 1.1:+71504862 MS.gene066243:CDS
CTGGTGGCATAGCAATCTAT+TGG 0.352724 1.1:+71504305 MS.gene066243:CDS
ATCAAAGATTGCCAAAATAA+AGG 0.357615 1.1:+71504499 MS.gene066243:CDS
GACCGTTACCGAATTTGTTT+AGG 0.359199 1.1:-71504402 None:intergenic
TTGGGCACTGCACTTGAAAC+AGG 0.362436 1.1:+71504805 MS.gene066243:CDS
TGGTGGCATAGCAATCTATT+GGG 0.384033 1.1:+71504306 MS.gene066243:CDS
GAAAATTGGCAACACCTTGC+AGG 0.393402 1.1:+71504703 MS.gene066243:CDS
ATAGCTTTCCTAAACAAATT+CGG 0.403876 1.1:+71504394 MS.gene066243:CDS
TGCCGGAATAAAACCACTTC+CGG 0.411953 1.1:-71504980 None:intergenic
GGTTGCGCATGCTTCGGATA+AGG 0.415599 1.1:-71504350 None:intergenic
CTACTCTTTATCTTCAATTG+AGG 0.422344 1.1:+71504561 MS.gene066243:CDS
AAGTCATCTTCAAGACCATT+AGG 0.427426 1.1:+71504631 MS.gene066243:CDS
TTATCCGAAGCATGCGCAAC+CGG 0.438403 1.1:+71504352 MS.gene066243:CDS
TCTACAGCGGCGGCCGGAAG+TGG 0.441284 1.1:+71504967 MS.gene066243:CDS
TCTAAAACAGCATCACCTAA+TGG 0.445847 1.1:-71504646 None:intergenic
CCGGCATCTACAGCGGCGGC+CGG 0.451172 1.1:+71504961 MS.gene066243:CDS
AGGTTTCATGATTTGCAGAC+TGG 0.456803 1.1:+71505075 MS.gene066243:CDS
GTGCATGAATTTGGAAGTGA+TGG 0.468935 1.1:-71504460 None:intergenic
TGGACTAGCACTGTTCCAGC+TGG 0.478277 1.1:+71504919 MS.gene066243:CDS
CTTGTTGCAACTTCCCATGC+TGG 0.478479 1.1:+71504286 MS.gene066243:CDS
CATGGAAGAAAAGTGTATCT+TGG 0.485127 1.1:+71504748 MS.gene066243:CDS
AGTATGGAGGAGTGATGCTT+TGG 0.487463 1.1:+71505049 MS.gene066243:CDS
GACCTGCGAGATTCATTTCA+GGG 0.492299 1.1:-71504429 None:intergenic
GATTGCTATGCCACCAGCAT+GGG 0.506152 1.1:-71504299 None:intergenic
CCGGCCGCCGCTGTAGATGC+CGG 0.519785 1.1:-71504961 None:intergenic
TCTGAAGTCAACACATCTGC+CGG 0.536946 1.1:-71504997 None:intergenic
TTATCTGGAATTGGACATTG+AGG 0.543911 1.1:-71504778 None:intergenic
GGTTTCATGATTTGCAGACT+GGG 0.544792 1.1:+71505076 MS.gene066243:CDS
GAGGAGTGATGCTTTGGTCA+AGG 0.544888 1.1:+71505055 MS.gene066243:CDS
CTTAAAGGGTATAGTAGACA+TGG 0.545260 1.1:+71504730 MS.gene066243:CDS
TTATGGAACACTTTCTTGGG+TGG 0.560429 1.1:+71504607 MS.gene066243:CDS
ACCCCTGAAATGAATCTCGC+AGG 0.567411 1.1:+71504427 MS.gene066243:CDS
TTCCTAAACAAATTCGGTAA+CGG 0.579160 1.1:+71504400 MS.gene066243:CDS
AGGGCTGCCGGCATCTACAG+CGG 0.585171 1.1:+71504954 MS.gene066243:CDS
TGGGGCCAAAATGGCAACGA+AGG 0.591522 1.1:+71504325 MS.gene066243:CDS
AGATTGCTATGCCACCAGCA+TGG 0.592121 1.1:-71504300 None:intergenic
TTTCCATTATATTGACAAGG+TGG 0.592596 1.1:-71504865 None:intergenic
GTTGCAACTTCCCATGCTGG+TGG 0.594762 1.1:+71504289 MS.gene066243:CDS
GGACTAGCACTGTTCCAGCT+GGG 0.601285 1.1:+71504920 MS.gene066243:CDS
GCTGCCGGCATCTACAGCGG+CGG 0.604171 1.1:+71504957 MS.gene066243:CDS
TTAGGAAAGCTATGTTAACA+TGG 0.604430 1.1:-71504384 None:intergenic
ATTTATTCCATGAATTCACA+AGG 0.612674 1.1:-71504897 None:intergenic
ACCCTTTAAGAAAACCTGCA+AGG 0.616480 1.1:-71504717 None:intergenic
ACCTGCGAGATTCATTTCAG+GGG 0.619333 1.1:-71504428 None:intergenic
ATGTTTCCATTATATTGACA+AGG 0.620396 1.1:-71504868 None:intergenic
TGCATGAATTTGGAAGTGAT+GGG 0.620811 1.1:-71504459 None:intergenic
TAGGAAAGCTATGTTAACAT+GGG 0.626283 1.1:-71504383 None:intergenic
GGGAAGTTGCAACAAGAGTG+AGG 0.646970 1.1:-71504279 None:intergenic
GGTGGCATAGCAATCTATTG+GGG 0.654630 1.1:+71504307 MS.gene066243:CDS
ATAAGAGACAACAAAGTGTG+AGG 0.661237 1.1:-71504247 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAGAAGTCAAAAAAGTA+TGG + chr1.1:71505033-71505052 MS.gene066243:CDS 20.0%
!!! AAACTTTGATTCCTTTATTT+TGG - chr1.1:71504513-71504532 None:intergenic 20.0%
!!! ATAGATTTTGATATTGAACT+TGG + chr1.1:71504673-71504692 MS.gene066243:CDS 20.0%
!!! ATCAAAGTTTTACTTTCAAT+TGG + chr1.1:71504523-71504542 MS.gene066243:CDS 20.0%
!!! GAAACGTTTCAAAATTTTTA+TGG + chr1.1:71504590-71504609 MS.gene066243:CDS 20.0%
! ATAGCTTTCCTAAACAAATT+CGG + chr1.1:71504394-71504413 MS.gene066243:CDS 25.0%
! ATCAAAGATTGCCAAAATAA+AGG + chr1.1:71504499-71504518 MS.gene066243:CDS 25.0%
! ATGTTTCCATTATATTGACA+AGG - chr1.1:71504871-71504890 None:intergenic 25.0%
! ATTTATTCCATGAATTCACA+AGG - chr1.1:71504900-71504919 None:intergenic 25.0%
! TTGTGAATTCATGGAATAAA+TGG + chr1.1:71504899-71504918 MS.gene066243:CDS 25.0%
!! AAAGTTTTACTTTCAATTGG+TGG + chr1.1:71504526-71504545 MS.gene066243:CDS 25.0%
!! ATTTTTATGGAACACTTTCT+TGG + chr1.1:71504603-71504622 MS.gene066243:CDS 25.0%
!! CCAATTCCAGATAAATTTTT+GGG + chr1.1:71504787-71504806 MS.gene066243:CDS 25.0%
!! TCCAATTCCAGATAAATTTT+TGG + chr1.1:71504786-71504805 MS.gene066243:CDS 25.0%
!! TTTTTATGGAACACTTTCTT+GGG + chr1.1:71504604-71504623 MS.gene066243:CDS 25.0%
!!! TTTTTTGACTTCTTAATCAC+AGG - chr1.1:71505030-71505049 None:intergenic 25.0%
AATCCACCTTGTCAATATAA+TGG + chr1.1:71504862-71504881 MS.gene066243:CDS 30.0%
CTACTCTTTATCTTCAATTG+AGG + chr1.1:71504561-71504580 MS.gene066243:CDS 30.0%
TAACTAACCTTGTGAATTCA+TGG + chr1.1:71504890-71504909 MS.gene066243:CDS 30.0%
TAGGAAAGCTATGTTAACAT+GGG - chr1.1:71504386-71504405 None:intergenic 30.0%
TTAGGAAAGCTATGTTAACA+TGG - chr1.1:71504387-71504406 None:intergenic 30.0%
TTCCTAAACAAATTCGGTAA+CGG + chr1.1:71504400-71504419 MS.gene066243:CDS 30.0%
TTTCCATTATATTGACAAGG+TGG - chr1.1:71504868-71504887 None:intergenic 30.0%
! AAGAAGTCAAAAAAGTATGG+AGG + chr1.1:71505036-71505055 MS.gene066243:CDS 30.0%
! CCCAAAAATTTATCTGGAAT+TGG - chr1.1:71504790-71504809 None:intergenic 30.0%
! CTGAATTTTGTGCATGAATT+TGG - chr1.1:71504472-71504491 None:intergenic 30.0%
!!! AGGTCTTTTTGACTTTGTTT+GGG + chr1.1:71504825-71504844 MS.gene066243:CDS 30.0%
AAGTCATCTTCAAGACCATT+AGG + chr1.1:71504631-71504650 MS.gene066243:CDS 35.0%
ATAAGAGACAACAAAGTGTG+AGG - chr1.1:71504250-71504269 None:intergenic 35.0%
GGGTATAGCACTTCTATTAT+TGG + chr1.1:71505096-71505115 MS.gene066243:CDS 35.0%
TGCATGAATTTGGAAGTGAT+GGG - chr1.1:71504462-71504481 None:intergenic 35.0%
! ACCTTGCAGGTTTTCTTAAA+GGG + chr1.1:71504716-71504735 MS.gene066243:CDS 35.0%
! CATGGAAGAAAAGTGTATCT+TGG + chr1.1:71504748-71504767 MS.gene066243:CDS 35.0%
! CTTAAAGGGTATAGTAGACA+TGG + chr1.1:71504730-71504749 MS.gene066243:CDS 35.0%
! GTTTTACTTTCAATTGGTGG+TGG + chr1.1:71504529-71504548 MS.gene066243:CDS 35.0%
! TCTAAAACAGCATCACCTAA+TGG - chr1.1:71504649-71504668 None:intergenic 35.0%
! TTATCTGGAATTGGACATTG+AGG - chr1.1:71504781-71504800 None:intergenic 35.0%
!! CAGCTGGGAAGATATTTTTA+GGG + chr1.1:71504935-71504954 MS.gene066243:CDS 35.0%
!!! CAGGTCTTTTTGACTTTGTT+TGG + chr1.1:71504824-71504843 MS.gene066243:CDS 35.0%
!!! GTTAACATGGGAGTATTTTC+CGG - chr1.1:71504374-71504393 None:intergenic 35.0%
!!! TTAGGTGATGCTGTTTTAGA+TGG + chr1.1:71504649-71504668 MS.gene066243:CDS 35.0%
AACTTGGTTCCACCGAAAAT+TGG + chr1.1:71504689-71504708 MS.gene066243:CDS 40.0%
CCTAAAAATATCTTCCCAGC+TGG - chr1.1:71504937-71504956 None:intergenic 40.0%
GCAGTGCCCAAAAATTTATC+TGG - chr1.1:71504796-71504815 None:intergenic 40.0%
GTGCATGAATTTGGAAGTGA+TGG - chr1.1:71504463-71504482 None:intergenic 40.0%
TGGTGGCATAGCAATCTATT+GGG + chr1.1:71504306-71504325 MS.gene066243:CDS 40.0%
TTATGGAACACTTTCTTGGG+TGG + chr1.1:71504607-71504626 MS.gene066243:CDS 40.0%
! ACCCTTTAAGAAAACCTGCA+AGG - chr1.1:71504720-71504739 None:intergenic 40.0%
! AGGTTTCATGATTTGCAGAC+TGG + chr1.1:71505075-71505094 MS.gene066243:CDS 40.0%
! CACCTTGCAGGTTTTCTTAA+AGG + chr1.1:71504715-71504734 MS.gene066243:CDS 40.0%
! CCAGCTGGGAAGATATTTTT+AGG + chr1.1:71504934-71504953 MS.gene066243:CDS 40.0%
! GACCGTTACCGAATTTGTTT+AGG - chr1.1:71504405-71504424 None:intergenic 40.0%
! GGTTTCATGATTTGCAGACT+GGG + chr1.1:71505076-71505095 MS.gene066243:CDS 40.0%
!! CTTTCAATTGGTGGTGGAAT+TGG + chr1.1:71504535-71504554 MS.gene066243:CDS 40.0%
!!! GAAGATATTTTTAGGGCTGC+CGG + chr1.1:71504942-71504961 MS.gene066243:CDS 40.0%
!!! TGCAAGGTGTTGCCAATTTT+CGG - chr1.1:71504704-71504723 None:intergenic 40.0%
ACCTGCGAGATTCATTTCAG+GGG - chr1.1:71504431-71504450 None:intergenic 45.0%
AGTATGGAGGAGTGATGCTT+TGG + chr1.1:71505049-71505068 MS.gene066243:CDS 45.0%
CTGGTGGCATAGCAATCTAT+TGG + chr1.1:71504305-71504324 MS.gene066243:CDS 45.0%
GAAAATTGGCAACACCTTGC+AGG + chr1.1:71504703-71504722 MS.gene066243:CDS 45.0%
GACCTGCGAGATTCATTTCA+GGG - chr1.1:71504432-71504451 None:intergenic 45.0%
GCAATCTATTGGGGCCAAAA+TGG + chr1.1:71504316-71504335 MS.gene066243:CDS 45.0%
GGTGGCATAGCAATCTATTG+GGG + chr1.1:71504307-71504326 MS.gene066243:CDS 45.0%
TGACCTGCGAGATTCATTTC+AGG - chr1.1:71504433-71504452 None:intergenic 45.0%
TGCCGGAATAAAACCACTTC+CGG - chr1.1:71504983-71505002 None:intergenic 45.0%
! TCTGAAGTCAACACATCTGC+CGG - chr1.1:71505000-71505019 None:intergenic 45.0%
!! AGGTTCCTTCGTTGCCATTT+TGG - chr1.1:71504333-71504352 None:intergenic 45.0%
!!! AAGGTGTTGCCAATTTTCGG+TGG - chr1.1:71504701-71504720 None:intergenic 45.0%
ACCCCTGAAATGAATCTCGC+AGG + chr1.1:71504427-71504446 MS.gene066243:CDS 50.0%
AGATTGCTATGCCACCAGCA+TGG - chr1.1:71504303-71504322 None:intergenic 50.0%
CTTGTTGCAACTTCCCATGC+TGG + chr1.1:71504286-71504305 MS.gene066243:CDS 50.0%
GAGGAGTGATGCTTTGGTCA+AGG + chr1.1:71505055-71505074 MS.gene066243:CDS 50.0%
GATTGCTATGCCACCAGCAT+GGG - chr1.1:71504302-71504321 None:intergenic 50.0%
GGGAAGTTGCAACAAGAGTG+AGG - chr1.1:71504282-71504301 None:intergenic 50.0%
TTATCCGAAGCATGCGCAAC+CGG + chr1.1:71504352-71504371 MS.gene066243:CDS 50.0%
! TTGGGCACTGCACTTGAAAC+AGG + chr1.1:71504805-71504824 MS.gene066243:CDS 50.0%
! TTTTCCGGTTGCGCATGCTT+CGG - chr1.1:71504359-71504378 None:intergenic 50.0%
!!! GGCCGGAAGTGGTTTTATTC+CGG + chr1.1:71504978-71504997 MS.gene066243:CDS 50.0%
GGTTGCGCATGCTTCGGATA+AGG - chr1.1:71504353-71504372 None:intergenic 55.0%
GTTGCAACTTCCCATGCTGG+TGG + chr1.1:71504289-71504308 MS.gene066243:CDS 55.0%
TGGGGCCAAAATGGCAACGA+AGG + chr1.1:71504325-71504344 MS.gene066243:CDS 55.0%
! GGACTAGCACTGTTCCAGCT+GGG + chr1.1:71504920-71504939 MS.gene066243:CDS 55.0%
!! TGGACTAGCACTGTTCCAGC+TGG + chr1.1:71504919-71504938 MS.gene066243:CDS 55.0%
AGGGCTGCCGGCATCTACAG+CGG + chr1.1:71504954-71504973 MS.gene066243:CDS 65.0%
GCTGCCGGCATCTACAGCGG+CGG + chr1.1:71504957-71504976 MS.gene066243:CDS 70.0%
TCTACAGCGGCGGCCGGAAG+TGG + chr1.1:71504967-71504986 MS.gene066243:CDS 70.0%
CCGGCATCTACAGCGGCGGC+CGG + chr1.1:71504961-71504980 MS.gene066243:CDS 75.0%
CCGGCCGCCGCTGTAGATGC+CGG - chr1.1:71504964-71504983 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 71504235 71505128 71504235 ID=MS.gene066243
chr1.1 mRNA 71504235 71505128 71504235 ID=MS.gene066243.t1;Parent=MS.gene066243
chr1.1 exon 71504235 71505128 71504235 ID=MS.gene066243.t1.exon1;Parent=MS.gene066243.t1
chr1.1 CDS 71504235 71505128 71504235 ID=cds.MS.gene066243.t1;Parent=MS.gene066243.t1
Gene Sequence

>MS.gene066243

ATGGCAAAAAATCCTCACACTTTGTTGTCTCTTATTCTTCTATTCCTCACTCTTGTTGCAACTTCCCATGCTGGTGGCATAGCAATCTATTGGGGCCAAAATGGCAACGAAGGAACCTTATCCGAAGCATGCGCAACCGGAAAATACTCCCATGTTAACATAGCTTTCCTAAACAAATTCGGTAACGGTCAAACCCCTGAAATGAATCTCGCAGGTCACTGCAACCCATCACTTCCAAATTCATGCACAAAATTCAGCTCAGAAATCAAAGATTGCCAAAATAAAGGAATCAAAGTTTTACTTTCAATTGGTGGTGGAATTGGAAGCTACTCTTTATCTTCAATTGAGGATGCAAGAAACGTTTCAAAATTTTTATGGAACACTTTCTTGGGTGGCAAGTCATCTTCAAGACCATTAGGTGATGCTGTTTTAGATGGAATAGATTTTGATATTGAACTTGGTTCCACCGAAAATTGGCAACACCTTGCAGGTTTTCTTAAAGGGTATAGTAGACATGGAAGAAAAGTGTATCTTGGTGCAGCTCCTCAATGTCCAATTCCAGATAAATTTTTGGGCACTGCACTTGAAACAGGTCTTTTTGACTTTGTTTGGGTACAATTTTACAATAATCCACCTTGTCAATATAATGGAAACATAACTAACCTTGTGAATTCATGGAATAAATGGACTAGCACTGTTCCAGCTGGGAAGATATTTTTAGGGCTGCCGGCATCTACAGCGGCGGCCGGAAGTGGTTTTATTCCGGCAGATGTGTTGACTTCAGAAATTTTACCTGTGATTAAGAAGTCAAAAAAGTATGGAGGAGTGATGCTTTGGTCAAGGTTTCATGATTTGCAGACTGGGTATAGCACTTCTATTATTGGAAGCGTGTGA

Protein sequence

>MS.gene066243.t1

MAKNPHTLLSLILLFLTLVATSHAGGIAIYWGQNGNEGTLSEACATGKYSHVNIAFLNKFGNGQTPEMNLAGHCNPSLPNSCTKFSSEIKDCQNKGIKVLLSIGGGIGSYSLSSIEDARNVSKFLWNTFLGGKSSSRPLGDAVLDGIDFDIELGSTENWQHLAGFLKGYSRHGRKVYLGAAPQCPIPDKFLGTALETGLFDFVWVQFYNNPPCQYNGNITNLVNSWNKWTSTVPAGKIFLGLPASTAAAGSGFIPADVLTSEILPVIKKSKKYGGVMLWSRFHDLQTGYSTSIIGSV