AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066447

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066447.t1 MTR_1g115500 81.893 243 13 1 1 243 1 212 1.66e-139 390
MS.gene066447.t1 MTR_3g066060 51.867 241 85 1 3 243 55 264 1.32e-86 258
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066447.t1 AT1G75270 64.344 244 55 2 1 243 1 213 2.96e-108 311
MS.gene066447.t1 AT1G19570 64.609 243 54 2 1 242 1 212 8.76e-108 310
MS.gene066447.t1 AT5G16710 52.720 239 82 1 3 241 49 256 1.72e-89 265
MS.gene066447.t1 AT1G19550 55.172 174 51 3 72 242 3 152 1.09e-59 186

Find 59 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTCAAGTTTGATGGTAAA+TGG 0.194555 1.2:+85645100 MS.gene066447:CDS
TTGGACTATTCCTGAGAATT+TGG 0.207787 1.2:+85645805 MS.gene066447:CDS
AAGAGAACCCTTTGGGAAAA+TGG 0.223837 1.2:-85644881 None:intergenic
ATTTCAGGGGATCAAATATA+TGG 0.237821 1.2:+85645484 MS.gene066447:intron
CTGAGATTTATTTCTGTTAC+CGG 0.289710 1.2:+85644816 MS.gene066447:CDS
CACAGCTGTTGATTTGAGTT+TGG 0.323954 1.2:+85645736 MS.gene066447:CDS
CTACTTCATGCATTGACCTT+TGG 0.348598 1.2:-85647496 None:intergenic
CCTGATTCTGATGTTATTGT+TGG 0.361346 1.2:+85645126 MS.gene066447:CDS
TGCTTTGGATGAACATCTTA+AGG 0.363226 1.2:+85645581 MS.gene066447:CDS
ACCACTAGATGGTACAGTTT+TGG 0.363899 1.2:-85645761 None:intergenic
CAAAGTTAAGAGAACCCTTT+GGG 0.367512 1.2:-85644888 None:intergenic
TAACAGGGTCCATTTGTTGC+TGG 0.379644 1.2:+85645704 MS.gene066447:intron
GTTAGCAGAACTCAATGCTT+TGG 0.381868 1.2:+85645566 MS.gene066447:CDS
GTTCCAGTGGTCAAGTTTGA+TGG 0.383379 1.2:+85645093 MS.gene066447:CDS
CCAAAGGGTTCTCTTAACTT+TGG 0.391179 1.2:+85644889 MS.gene066447:CDS
TGGACCTTTGCCAAATTCTC+AGG 0.406140 1.2:-85645815 None:intergenic
AACAGGGTCCATTTGTTGCT+GGG 0.421851 1.2:+85645705 MS.gene066447:intron
GACATACTCCTCTGCAGCCT+TGG 0.421971 1.2:-85647458 None:intergenic
GCAGAGGAGTATGTCATTGC+TGG 0.422618 1.2:+85647466 MS.gene066447:CDS
GGAGAAACAAGAGATGGTTC+AGG 0.422879 1.2:-85645168 None:intergenic
CCAAAGTTAAGAGAACCCTT+TGG 0.428562 1.2:-85644889 None:intergenic
TTAATCAAATGGATGTTGTA+TGG 0.432983 1.2:-85644926 None:intergenic
AGAGTACCATTGGGGTTTGT+CGG 0.436593 1.2:-85644954 None:intergenic
TGTCGGAGATATTAATCAAA+TGG 0.447813 1.2:-85644937 None:intergenic
ACCTTCTCCCCAGCAACAAA+TGG 0.454458 1.2:-85645713 None:intergenic
GTTGGTGCTCCAACTGTTCT+CGG 0.458021 1.2:+85644758 MS.gene066447:CDS
GGTCAAGTTTGATGGTAAAT+GGG 0.460396 1.2:+85645101 MS.gene066447:CDS
TGAATCCTTGCTCTTCAGAA+AGG 0.466931 1.2:-85645520 None:intergenic
CCAACAATAACATCAGAATC+AGG 0.477453 1.2:-85645126 None:intergenic
GGCTCTTGAGGTTGCTGTCA+AGG 0.478726 1.2:+85644730 MS.gene066447:CDS
ACCACTGGAACCTTCCCTCC+AGG 0.482366 1.2:-85645081 None:intergenic
AAGAGCAAGGATTCAAATGA+TGG 0.484327 1.2:+85645528 MS.gene066447:CDS
TCCATTTGTTGCTGGGGAGA+AGG 0.485126 1.2:+85645712 MS.gene066447:CDS
GACCAAGTGCTACCACTAGA+TGG 0.490749 1.2:-85645772 None:intergenic
CGTGACCTTTCTGAAGAGCA+AGG 0.493675 1.2:+85645515 MS.gene066447:CDS
TTACCATCAAACTTGACCAC+TGG 0.501479 1.2:-85645096 None:intergenic
GTTGCTGTCAAGGCTGCTGT+TGG 0.509413 1.2:+85644740 MS.gene066447:CDS
CACTTGGTCACTTCAAGAGT+TGG 0.517047 1.2:+85645786 MS.gene066447:CDS
TTTCAGGGGATCAAATATAT+GGG 0.518607 1.2:+85645485 MS.gene066447:intron
TACACTGAGGAAAATTGAGC+AGG 0.548119 1.2:-85645189 None:intergenic
AGGAGTATGTCATTGCTGGA+TGG 0.548576 1.2:+85647470 MS.gene066447:CDS
CATTGCTGGATGGGCACCAA+AGG 0.555766 1.2:+85647480 MS.gene066447:CDS
ACCATCGCAAATGGCTCTTG+AGG 0.559050 1.2:+85644718 None:intergenic
GGAAGTGAATCCTGGAGGGA+AGG 0.560932 1.2:+85645071 MS.gene066447:CDS
GAGAAACAAGAGATGGTTCA+GGG 0.568330 1.2:-85645167 None:intergenic
GGAGTATGTCATTGCTGGAT+GGG 0.575558 1.2:+85647471 MS.gene066447:CDS
CGAGAAAACCAAGGCTGCAG+AGG 0.575652 1.2:+85647450 MS.gene066447:CDS
GTACAGTCTCCGAGAACAGT+TGG 0.596027 1.2:-85644767 None:intergenic
ACAGGGTCCATTTGTTGCTG+GGG 0.596245 1.2:+85645706 MS.gene066447:intron
TACCATCTAGTGGTAGCACT+TGG 0.606458 1.2:+85645770 MS.gene066447:CDS
TCCTGGAGGGAAGGTTCCAG+TGG 0.613379 1.2:+85645080 MS.gene066447:CDS
TATTCCTGAGAATTTGGCAA+AGG 0.623001 1.2:+85645811 MS.gene066447:CDS
TTTGTACCTTGATGTAGTTG+TGG 0.632536 1.2:-85645835 None:intergenic
AAAGGTCCACAACTACATCA+AGG 0.634770 1.2:+85645829 MS.gene066447:CDS
TGAGCAGGAGAAACAAGAGA+TGG 0.641215 1.2:-85645174 None:intergenic
TTGAATTTAGAGTACCATTG+GGG 0.655225 1.2:-85644962 None:intergenic
ACCAAAACTGTACCATCTAG+TGG 0.659489 1.2:+85645760 MS.gene066447:CDS
ATATCTCCGACAAACCCCAA+TGG 0.672245 1.2:+85644948 MS.gene066447:CDS
TGAATCATTCGAGAAAACCA+AGG 0.685046 1.2:+85647441 MS.gene066447:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGATAAATAATATATTACA+CGG - chr1.2:85647191-85647210 None:intergenic 10.0%
!!! AATCAAATTTTGAAATTTAA+AGG - chr1.2:85647058-85647077 None:intergenic 10.0%
!!! ATTTTTTTTTTTAGAGAAAA+GGG - chr1.2:85646643-85646662 None:intergenic 10.0%
!! ATAAATTCACATATATACAT+TGG - chr1.2:85645607-85645626 None:intergenic 15.0%
!!! AAATTTTGAAATTTAAAGGA+GGG - chr1.2:85647054-85647073 None:intergenic 15.0%
!!! GATTTTTTTTTTTAGAGAAA+AGG - chr1.2:85646644-85646663 None:intergenic 15.0%
!!! TTAATAGGTTATATAACTTT+TGG + chr1.2:85647130-85647149 MS.gene066447:intron 15.0%
!!! TTTATTTATTTGTAGGTTTT+TGG + chr1.2:85645050-85645069 MS.gene066447:intron 15.0%
!!! TTTGAAAGTATAAAAATAGA+GGG - chr1.2:85645228-85645247 None:intergenic 15.0%
!!! TTTTATTTCTTTGAATTTTG+TGG + chr1.2:85645015-85645034 MS.gene066447:intron 15.0%
!!! TTTTTTTTTTTAGAGAAAAG+GGG - chr1.2:85646642-85646661 None:intergenic 15.0%
!! ACATTTCCAATATCAAATTA+TGG + chr1.2:85646244-85646263 MS.gene066447:intron 20.0%
!! CATTTCCAATATCAAATTAT+GGG + chr1.2:85646245-85646264 MS.gene066447:intron 20.0%
!! TATATGAATTGATTGATTGA+TGG + chr1.2:85647384-85647403 MS.gene066447:intron 20.0%
!! TATGCTAAAAAAATGTTACA+TGG - chr1.2:85646538-85646557 None:intergenic 20.0%
!!! AATTTTGAAATTTAAAGGAG+GGG - chr1.2:85647053-85647072 None:intergenic 20.0%
!!! ATGTTACTTTCTATATTTTG+TGG + chr1.2:85645294-85645313 MS.gene066447:intron 20.0%
!!! CAAATTTTGAAATTTAAAGG+AGG - chr1.2:85647055-85647074 None:intergenic 20.0%
!!! GTTTGAAAGTATAAAAATAG+AGG - chr1.2:85645229-85645248 None:intergenic 20.0%
!!! TGTTACTTTCTATATTTTGT+GGG + chr1.2:85645295-85645314 MS.gene066447:intron 20.0%
!!! TTTCATTTGTTTTCATTTTC+AGG + chr1.2:85644857-85644876 MS.gene066447:CDS 20.0%
!!! TTTCATTTTTTTGGATAAAG+TGG - chr1.2:85646666-85646685 None:intergenic 20.0%
! AAATACATCAATACTCCTAA+AGG - chr1.2:85647244-85647263 None:intergenic 25.0%
! AAGTACAACAAAAAAACAGT+TGG - chr1.2:85646011-85646030 None:intergenic 25.0%
! ATGGATGTAATCTTATCTTA+TGG + chr1.2:85645871-85645890 MS.gene066447:intron 25.0%
! ATTGTAGAAGTTGACAATTA+GGG + chr1.2:85646824-85646843 MS.gene066447:intron 25.0%
! CAAGTGATTAAATTTATCTC+TGG + chr1.2:85646560-85646579 MS.gene066447:intron 25.0%
! GTTAATTAGCTATCAATTAC+AGG - chr1.2:85645662-85645681 None:intergenic 25.0%
! TCATAAATCAAAGAAACAAC+AGG - chr1.2:85646918-85646937 None:intergenic 25.0%
! TCATCACATCATTAGTTAAT+AGG + chr1.2:85647115-85647134 MS.gene066447:intron 25.0%
! TTAATCAAATGGATGTTGTA+TGG - chr1.2:85644929-85644948 None:intergenic 25.0%
! TTATGAAGAAAATTCAAGTG+TGG - chr1.2:85646040-85646059 None:intergenic 25.0%
!! ATTGGATAGAGATTTCTTTT+AGG + chr1.2:85646439-85646458 MS.gene066447:intron 25.0%
!! GTTGTAGGTATTGTAAATTA+AGG - chr1.2:85647303-85647322 None:intergenic 25.0%
!!! AAGCATGCTTTTCATTTTTT+TGG - chr1.2:85646675-85646694 None:intergenic 25.0%
!!! GGGTTTTAAATCTTACATAT+TGG + chr1.2:85646421-85646440 MS.gene066447:intron 25.0%
!!! TACAAAGTTGTTTTCTGTAA+TGG + chr1.2:85645852-85645871 MS.gene066447:intron 25.0%
!!! TTTTTTTCAGCAAATGCAAA+AGG - chr1.2:85647331-85647350 None:intergenic 25.0%
AATCGTGAAAAATGTAGAAC+CGG - chr1.2:85644838-85644857 None:intergenic 30.0%
AATTGTCAACTTCTACAATC+TGG - chr1.2:85646823-85646842 None:intergenic 30.0%
ATATATCGAGTTAGACCTTT+AGG + chr1.2:85647226-85647245 MS.gene066447:intron 30.0%
ATCTTCAGACTTAAAGTTGA+TGG - chr1.2:85646997-85647016 None:intergenic 30.0%
ATTTCAGGGGATCAAATATA+TGG + chr1.2:85645484-85645503 MS.gene066447:intron 30.0%
GATATGGATGAAAGATGTAT+GGG - chr1.2:85646325-85646344 None:intergenic 30.0%
GATTGTAGAAGTTGACAATT+AGG + chr1.2:85646823-85646842 MS.gene066447:intron 30.0%
GCAAACCCATAATTTGATAT+TGG - chr1.2:85646253-85646272 None:intergenic 30.0%
TAAAGTCTGAACTTAAAGCT+GGG + chr1.2:85646401-85646420 MS.gene066447:intron 30.0%
TACCTAAGAGATTCATTGTA+TGG - chr1.2:85647272-85647291 None:intergenic 30.0%
TAGTTCACATTAACTAGTCA+TGG + chr1.2:85646870-85646889 MS.gene066447:intron 30.0%
TATATTACACGGATTCAATG+TGG - chr1.2:85647180-85647199 None:intergenic 30.0%
TGATATGGATGAAAGATGTA+TGG - chr1.2:85646326-85646345 None:intergenic 30.0%
TGTCGGAGATATTAATCAAA+TGG - chr1.2:85644940-85644959 None:intergenic 30.0%
TTAAAGTCTGAACTTAAAGC+TGG + chr1.2:85646400-85646419 MS.gene066447:intron 30.0%
TTGAATACCATCTTGCAAAT+GGG - chr1.2:85645264-85645283 None:intergenic 30.0%
TTTCAGGGGATCAAATATAT+GGG + chr1.2:85645485-85645504 MS.gene066447:intron 30.0%
! AGTTGAATTTAGAGTACCAT+TGG - chr1.2:85644967-85644986 None:intergenic 30.0%
! GTTGAATTTAGAGTACCATT+GGG - chr1.2:85644966-85644985 None:intergenic 30.0%
! TTGAATTTAGAGTACCATTG+GGG - chr1.2:85644965-85644984 None:intergenic 30.0%
! TTTCTATATTTTGTGGGATG+AGG + chr1.2:85645301-85645320 MS.gene066447:intron 30.0%
!! AATTATGCGTGGCTATTTTA+GGG + chr1.2:85646466-85646485 MS.gene066447:intron 30.0%
!! CTGAGATTTATTTCTGTTAC+CGG + chr1.2:85644816-85644835 MS.gene066447:CDS 30.0%
!! GTACTTCAATCGTTTTGATA+TGG - chr1.2:85646341-85646360 None:intergenic 30.0%
!!! GTATACATGCTTGTTTTGAT+TGG + chr1.2:85645363-85645382 MS.gene066447:intron 30.0%
!!! TCTTTGTTTTAAAGCAAAGC+TGG + chr1.2:85646172-85646191 MS.gene066447:intron 30.0%
!!! TTTTTTTTAGAGAAAAGGGG+AGG - chr1.2:85646639-85646658 None:intergenic 30.0%
AAGAGCAAGGATTCAAATGA+TGG + chr1.2:85645528-85645547 MS.gene066447:CDS 35.0%
AATTAACCACACTAACATGC+TGG + chr1.2:85645675-85645694 MS.gene066447:intron 35.0%
ACGCAAAAGACATGACTAAA+AGG - chr1.2:85645900-85645919 None:intergenic 35.0%
CAAAGTTAAGAGAACCCTTT+GGG - chr1.2:85644891-85644910 None:intergenic 35.0%
CCAACAATAACATCAGAATC+AGG - chr1.2:85645129-85645148 None:intergenic 35.0%
CCTGATTCTGATGTTATTGT+TGG + chr1.2:85645126-85645145 MS.gene066447:CDS 35.0%
CGCAAAAGACATGACTAAAA+GGG - chr1.2:85645899-85645918 None:intergenic 35.0%
CTTGAATACCATCTTGCAAA+TGG - chr1.2:85645265-85645284 None:intergenic 35.0%
GGTGAGTTAAGAAATAGAGA+AGG - chr1.2:85646618-85646637 None:intergenic 35.0%
GTCCATACAATGAATCTCTT+AGG + chr1.2:85647267-85647286 MS.gene066447:intron 35.0%
TATTCCTGAGAATTTGGCAA+AGG + chr1.2:85645811-85645830 MS.gene066447:CDS 35.0%
TCACGATGTTCACAATTTCA+GGG + chr1.2:85645470-85645489 MS.gene066447:intron 35.0%
TGAATACCATCTTGCAAATG+GGG - chr1.2:85645263-85645282 None:intergenic 35.0%
TGAATCATTCGAGAAAACCA+AGG + chr1.2:85647441-85647460 MS.gene066447:CDS 35.0%
TGTAGTCAACATAAAGTGTG+TGG + chr1.2:85646101-85646120 MS.gene066447:intron 35.0%
TTCACAGCTGTTCAATTCAA+TGG - chr1.2:85645925-85645944 None:intergenic 35.0%
TTCACGATGTTCACAATTTC+AGG + chr1.2:85645469-85645488 MS.gene066447:intron 35.0%
TTGGACTATTCCTGAGAATT+TGG + chr1.2:85645805-85645824 MS.gene066447:CDS 35.0%
TTTGTACCTTGATGTAGTTG+TGG - chr1.2:85645838-85645857 None:intergenic 35.0%
! AGTTCAATGAAGCATCAGTA+AGG - chr1.2:85646767-85646786 None:intergenic 35.0%
! ATGCAAAAGGCTTTAGTTGT+AGG - chr1.2:85647318-85647337 None:intergenic 35.0%
! CAATTTGCTTCTGGAAAGAT+TGG - chr1.2:85646212-85646231 None:intergenic 35.0%
! GGTCAAGTTTGATGGTAAAT+GGG + chr1.2:85645101-85645120 MS.gene066447:CDS 35.0%
! GTTCAATGAAGCATCAGTAA+GGG - chr1.2:85646766-85646785 None:intergenic 35.0%
! TGCTTTGGATGAACATCTTA+AGG + chr1.2:85645581-85645600 MS.gene066447:CDS 35.0%
! TGGTCAAGTTTGATGGTAAA+TGG + chr1.2:85645100-85645119 MS.gene066447:CDS 35.0%
! TGTGAACATCAATTTGCTTC+TGG - chr1.2:85646221-85646240 None:intergenic 35.0%
!! CAATTATGCGTGGCTATTTT+AGG + chr1.2:85646465-85646484 MS.gene066447:intron 35.0%
!! CATGCTTTTCTTATTTGCCA+GGG + chr1.2:85646688-85646707 MS.gene066447:intron 35.0%
!! TGCTGGTGTCATGAAAAAAT+TGG + chr1.2:85646279-85646298 MS.gene066447:intron 35.0%
!! TGGTGTCATGAAAAAATTGG+TGG + chr1.2:85646282-85646301 MS.gene066447:intron 35.0%
!! TTTTAGGTCTCAATTATGCG+TGG + chr1.2:85646455-85646474 MS.gene066447:intron 35.0%
AAAGGTCCACAACTACATCA+AGG + chr1.2:85645829-85645848 MS.gene066447:CDS 40.0%
ACCAAAACTGTACCATCTAG+TGG + chr1.2:85645760-85645779 MS.gene066447:CDS 40.0%
ACTATTCCCCATTTGCAAGA+TGG + chr1.2:85645254-85645273 MS.gene066447:intron 40.0%
CACGATGTTCACAATTTCAG+GGG + chr1.2:85645471-85645490 MS.gene066447:intron 40.0%
CCAAAGGGTTCTCTTAACTT+TGG + chr1.2:85644889-85644908 MS.gene066447:CDS 40.0%
CCAAAGTTAAGAGAACCCTT+TGG - chr1.2:85644892-85644911 None:intergenic 40.0%
GAGAAACAAGAGATGGTTCA+GGG - chr1.2:85645170-85645189 None:intergenic 40.0%
GGATAATTAAGAGTGAACCC+TGG - chr1.2:85646708-85646727 None:intergenic 40.0%
GTCAACATAAAGTGTGTGGA+AGG + chr1.2:85646105-85646124 MS.gene066447:intron 40.0%
GTTAGCAGAACTCAATGCTT+TGG + chr1.2:85645566-85645585 MS.gene066447:CDS 40.0%
TACACTGAGGAAAATTGAGC+AGG - chr1.2:85645192-85645211 None:intergenic 40.0%
TATCCTGCTTGACATGTACA+TGG + chr1.2:85646723-85646742 MS.gene066447:intron 40.0%
TGAATCCTTGCTCTTCAGAA+AGG - chr1.2:85645523-85645542 None:intergenic 40.0%
TGATGTGATGTCATACACTG+AGG - chr1.2:85645205-85645224 None:intergenic 40.0%
TGGATGAAAGATGTATGGGA+AGG - chr1.2:85646321-85646340 None:intergenic 40.0%
TTACCATCAAACTTGACCAC+TGG - chr1.2:85645099-85645118 None:intergenic 40.0%
! AAGAGAACCCTTTGGGAAAA+TGG - chr1.2:85644884-85644903 None:intergenic 40.0%
! ACCACTAGATGGTACAGTTT+TGG - chr1.2:85645764-85645783 None:intergenic 40.0%
! TGCTTGGCAATGAGAACTTT+TGG + chr1.2:85645968-85645987 MS.gene066447:intron 40.0%
! TTCAGGTCCATTTTCCCAAA+GGG + chr1.2:85644874-85644893 MS.gene066447:CDS 40.0%
!! AACATGCTGGAGGTATTAAC+AGG + chr1.2:85645688-85645707 MS.gene066447:intron 40.0%
!! AACTGCATGATGGCATTGAT+TGG + chr1.2:85645945-85645964 MS.gene066447:intron 40.0%
!! ACATGCTGGAGGTATTAACA+GGG + chr1.2:85645689-85645708 MS.gene066447:intron 40.0%
!! AGGTTTTTGGAAGTGAATCC+TGG + chr1.2:85645063-85645082 MS.gene066447:intron 40.0%
!! CACAGCTGTTGATTTGAGTT+TGG + chr1.2:85645736-85645755 MS.gene066447:CDS 40.0%
!! GCATGCTTTTCTTATTTGCC+AGG + chr1.2:85646687-85646706 MS.gene066447:intron 40.0%
!! TTTCAGGTCCATTTTCCCAA+AGG + chr1.2:85644873-85644892 MS.gene066447:CDS 40.0%
!! TTTTGGAAGTGAATCCTGGA+GGG + chr1.2:85645067-85645086 MS.gene066447:CDS 40.0%
!! TTTTTGGAAGTGAATCCTGG+AGG + chr1.2:85645066-85645085 MS.gene066447:CDS 40.0%
ATACCTCCAGCATGTTAGTG+TGG - chr1.2:85645684-85645703 None:intergenic 45.0%
ATATCTCCGACAAACCCCAA+TGG + chr1.2:85644948-85644967 MS.gene066447:CDS 45.0%
CACTTGGTCACTTCAAGAGT+TGG + chr1.2:85645786-85645805 MS.gene066447:CDS 45.0%
CAGCTGTGAAAACTGCATGA+TGG + chr1.2:85645935-85645954 MS.gene066447:intron 45.0%
CTTCCATGTACATGTCAAGC+AGG - chr1.2:85646729-85646748 None:intergenic 45.0%
GGAGAAACAAGAGATGGTTC+AGG - chr1.2:85645171-85645190 None:intergenic 45.0%
GGAGTATGTCATTGCTGGAT+GGG + chr1.2:85647471-85647490 MS.gene066447:CDS 45.0%
GTTCCAGTGGTCAAGTTTGA+TGG + chr1.2:85645093-85645112 MS.gene066447:CDS 45.0%
TAACCACACTAACATGCTGG+AGG + chr1.2:85645678-85645697 MS.gene066447:intron 45.0%
TACCATCTAGTGGTAGCACT+TGG + chr1.2:85645770-85645789 MS.gene066447:CDS 45.0%
TGAGCAGGAGAAACAAGAGA+TGG - chr1.2:85645177-85645196 None:intergenic 45.0%
TGGACCTTTGCCAAATTCTC+AGG - chr1.2:85645818-85645837 None:intergenic 45.0%
TTGTGGGATGAGGAATCTGA+GGG + chr1.2:85645311-85645330 MS.gene066447:intron 45.0%
TTTGTGGGATGAGGAATCTG+AGG + chr1.2:85645310-85645329 MS.gene066447:intron 45.0%
! AACAGGGTCCATTTGTTGCT+GGG + chr1.2:85645705-85645724 MS.gene066447:intron 45.0%
! AGAGTACCATTGGGGTTTGT+CGG - chr1.2:85644957-85644976 None:intergenic 45.0%
! AGGAGTATGTCATTGCTGGA+TGG + chr1.2:85647470-85647489 MS.gene066447:CDS 45.0%
! TAACAGGGTCCATTTGTTGC+TGG + chr1.2:85645704-85645723 MS.gene066447:intron 45.0%
!! TATGGGTTTGCTATGCTTGC+TGG + chr1.2:85646262-85646281 MS.gene066447:intron 45.0%
!! TGATGGCATTGATTGGTGCT+TGG + chr1.2:85645952-85645971 MS.gene066447:intron 45.0%
!! TTAAAAATTTAACAAAAATA+AGG - chr1.2:85645639-85645658 None:intergenic 5.0%
!! TTATTTATTTATTTATTTGT+AGG + chr1.2:85645043-85645062 MS.gene066447:intron 5.0%
ACCTTCTCCCCAGCAACAAA+TGG - chr1.2:85645716-85645735 None:intergenic 50.0%
GACCAAGTGCTACCACTAGA+TGG - chr1.2:85645775-85645794 None:intergenic 50.0%
GTACAGTCTCCGAGAACAGT+TGG - chr1.2:85644770-85644789 None:intergenic 50.0%
! ACAGGGTCCATTTGTTGCTG+GGG + chr1.2:85645706-85645725 MS.gene066447:intron 50.0%
! CGTGACCTTTCTGAAGAGCA+AGG + chr1.2:85645515-85645534 MS.gene066447:CDS 50.0%
! GCAGAGGAGTATGTCATTGC+TGG + chr1.2:85647466-85647485 MS.gene066447:CDS 50.0%
! GTTGGTGCTCCAACTGTTCT+CGG + chr1.2:85644758-85644777 MS.gene066447:CDS 50.0%
! TCCATTTGTTGCTGGGGAGA+AGG + chr1.2:85645712-85645731 MS.gene066447:CDS 50.0%
AGGGGAAGAAGACACTGCAG+TGG - chr1.2:85647035-85647054 None:intergenic 55.0%
CATTGCTGGATGGGCACCAA+AGG + chr1.2:85647480-85647499 MS.gene066447:CDS 55.0%
CGAGAAAACCAAGGCTGCAG+AGG + chr1.2:85647450-85647469 MS.gene066447:CDS 55.0%
GACATACTCCTCTGCAGCCT+TGG - chr1.2:85647461-85647480 None:intergenic 55.0%
GAGGAATCTGAGGGAGACTG+TGG + chr1.2:85645320-85645339 MS.gene066447:intron 55.0%
GGAAGTGAATCCTGGAGGGA+AGG + chr1.2:85645071-85645090 MS.gene066447:CDS 55.0%
GGCTCTTGAGGTTGCTGTCA+AGG + chr1.2:85644730-85644749 MS.gene066447:CDS 55.0%
GTTGCTGTCAAGGCTGCTGT+TGG + chr1.2:85644740-85644759 MS.gene066447:CDS 55.0%
TGTGTACACCTGCACGCACA+AGG + chr1.2:85646971-85646990 MS.gene066447:intron 55.0%
ACCACTGGAACCTTCCCTCC+AGG - chr1.2:85645084-85645103 None:intergenic 60.0%
TCCTGGAGGGAAGGTTCCAG+TGG + chr1.2:85645080-85645099 MS.gene066447:CDS 60.0%
!! GTTGATGGCCTTGTGCGTGC+AGG - chr1.2:85646982-85647001 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 85644728 85647513 85644728 ID=MS.gene066447
chr1.2 mRNA 85644728 85647513 85644728 ID=MS.gene066447.t1;Parent=MS.gene066447
chr1.2 exon 85644728 85644969 85644728 ID=MS.gene066447.t1.exon1;Parent=MS.gene066447.t1
chr1.2 CDS 85644728 85644969 85644728 ID=cds.MS.gene066447.t1;Parent=MS.gene066447.t1
chr1.2 exon 85645065 85645208 85645065 ID=MS.gene066447.t1.exon2;Parent=MS.gene066447.t1
chr1.2 CDS 85645065 85645208 85645065 ID=cds.MS.gene066447.t1;Parent=MS.gene066447.t1
chr1.2 exon 85645491 85645608 85645491 ID=MS.gene066447.t1.exon3;Parent=MS.gene066447.t1
chr1.2 CDS 85645491 85645608 85645491 ID=cds.MS.gene066447.t1;Parent=MS.gene066447.t1
chr1.2 exon 85645710 85645850 85645710 ID=MS.gene066447.t1.exon4;Parent=MS.gene066447.t1
chr1.2 CDS 85645710 85645850 85645710 ID=cds.MS.gene066447.t1;Parent=MS.gene066447.t1
chr1.2 exon 85647427 85647513 85647427 ID=MS.gene066447.t1.exon5;Parent=MS.gene066447.t1
chr1.2 CDS 85647427 85647513 85647427 ID=cds.MS.gene066447.t1;Parent=MS.gene066447.t1
Gene Sequence

>MS.gene066447

ATGGCTCTTGAGGTTGCTGTCAAGGCTGCTGTTGGTGCTCCAACTGTTCTCGGAGACTGTACGTCTCTGTCTCTGTCTCTCTCTCTCTCTGAGATTTATTTCTGTTACCGGTTCTACATTTTTCACGATTTTCATTTGTTTTCATTTTCAGGTCCATTTTCCCAAAGGGTTCTCTTAACTTTGGAAGAGAAGAAGATTCCATACAACATCCATTTGATTAATATCTCCGACAAACCCCAATGGTACTCTAAATTCAACTCTATTTATTTATACTTTTTTTATTTATTTTTTATTTCTTTGAATTTTGTGGCTTATTTATTTATTTATTTATTTGTAGGTTTTTGGAAGTGAATCCTGGAGGGAAGGTTCCAGTGGTCAAGTTTGATGGTAAATGGGTTCCTGATTCTGATGTTATTGTTGGAATTCTTGAAGACAAATACCCTGAACCATCTCTTGTTTCTCCTGCTCAATTTTCCTCAGTGTATGACATCACATCACCCTCTATTTTTATACTTTCAAACTTATGACTATTCCCCATTTGCAAGATGGTATTCAAGATTAACAAAATGTTACTTTCTATATTTTGTGGGATGAGGAATCTGAGGGAGACTGTGGTTATGTCAACTATTAAATTAGTATACATGCTTGTTTTGATTGGCTTATTTCAACATATCTACTTGCTTAAACACTGTTGAAACTGTTTGAGAGAGCTTATATAAATATCTTATAGAAATTTTGTTTTTCACGATGTTCACAATTTCAGGGGATCAAATATATGGGATACTTTCGTGACCTTTCTGAAGAGCAAGGATTCAAATGATGGAACAGAACAAGCTTTGTTAGCAGAACTCAATGCTTTGGATGAACATCTTAAGGCCAATGTATATATGTGAATTTATTGTCGACAACCTTATTTTTGTTAAATTTTTAACCTGTAATTGATAGCTAATTAACCACACTAACATGCTGGAGGTATTAACAGGGTCCATTTGTTGCTGGGGAGAAGGTCACAGCTGTTGATTTGAGTTTGGCACCAAAACTGTACCATCTAGTGGTAGCACTTGGTCACTTCAAGAGTTGGACTATTCCTGAGAATTTGGCAAAGGTCCACAACTACATCAAGGTACAAAGTTGTTTTCTGTAATGGATGTAATCTTATCTTATGGTTCCCTTTTAGTCATGTCTTTTGCGTTGCCATTGAATTGAACAGCTGTGAAAACTGCATGATGGCATTGATTGGTGCTTGGCAATGAGAACTTTTGGTTGTCTAATTTGTCATGCCAACTGTTTTTTTGTTGTACTTTCTATACCACACTTGAATTTTCTTCATAATAGCACACAAATTGTTTATTTATCTGCTACTTATAAAAATTTGTAGTCAACATAAAGTGTGTGGAAGGAAAAACACTACAACTCAAGAAGCAAACATAATTGAAAAGACTTTTCTTTGTTTTAAAGCAAAGCTGGACTTATTTATGTCACCAATCTTTCCAGAAGCAAATTGATGTTCACAAAAACATTTCCAATATCAAATTATGGGTTTGCTATGCTTGCTGGTGTCATGAAAAAATTGGTGGTTAAATATTAATTCCTTCCCATACATCTTTCATCCATATCAAAACGATTGAAGTACATGCTTTAAAGATAAAATAAATCTTTTTAGCTTGAATTTTTAAAGTCTGAACTTAAAGCTGGGTTTTAAATCTTACATATTGGATAGAGATTTCTTTTAGGTCTCAATTATGCGTGGCTATTTTAGGGAAGTTTCTCTTTCAAACATTCTGTAATCTGTATTAGACTTAGACTGCCATGTAACATTTTTTTAGCATATTCAAGTGATTAAATTTATCTCTGGATGTCTATATGTTTTATGATTTTGATAGCTTTCCTTCTCTATTTCTTAACTCACCTCCCCTTTTCTCTAAAAAAAAAAATCCACTTTATCCAAAAAAATGAAAAGCATGCTTTTCTTATTTGCCAGGGTTCACTCTTAATTATCCTGCTTGACATGTACATGGAAGTATTGATACTTCAACCCTTACTGATGCTTCATTGAACTTTTTCTTTCTTGTCTTAGCAACAAATATTGTTGCCAGATTGTAGAAGTTGACAATTAGGGTAGTATGTCACGTTCATTCTTTTTAGTTCACATTAACTAGTCATGGACAAATTTTTATGCATGTTCTTCCTGTTGTTTCTTTGATTTATGATTACTGTCTTCTGCTGCTCTGAGCTGTGATTATTGTGTACACCTGCACGCACAAGGCCATCAACTTTAAGTCTGAAGATGTTTTCATACTATTACCACTGCAGTGTCTTCTTCCCCTCCTTTAAATTTCAAAATTTGATTGAGCTATGATTTTCTATGCAACAAATTGTTAGCTTGATCATCACATCATTAGTTAATAGGTTATATAACTTTTGGTGTGATAGTTCATGAAGCTATTTACCACATTGAATCCGTGTAATATATTATTTATCAAATGTGTTGATGCGTTATATATCGAGTTAGACCTTTAGGAGTATTGATGTATTTAAAGTCCATACAATGAATCTCTTAGGTAATTTAGTGCCTTAATTTACAATACCTACAACTAAAGCCTTTTGCATTTGCTGAAAAAAAGATGTAATTGCTTGTTTGAATTGTATTTAATTGTATATGAATTGATTGATTGATGGATAATTATATTATACTGCAGTTGCTCTTCTCTCGTGAATCATTCGAGAAAACCAAGGCTGCAGAGGAGTATGTCATTGCTGGATGGGCACCAAAGGTCAATGCATGA
Protein sequence

>MS.gene066447.t1

MALEVAVKAAVGAPTVLGDCTSLSLSLSLSEIYFCYRFYIFHDFHLFSFSGPFSQRVLLTLEEKKIPYNIHLINISDKPQWFLEVNPGGKVPVVKFDGKWVPDSDVIVGILEDKYPEPSLVSPAQFSSVGSNIWDTFVTFLKSKDSNDGTEQALLAELNALDEHLKANGPFVAGEKVTAVDLSLAPKLYHLVVALGHFKSWTIPENLAKVHNYIKLLFSRESFEKTKAAEEYVIAGWAPKVNA