AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066676

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066676.t1 MTR_4g022570 99.359 156 1 0 12 167 64 219 6.50e-113 320
MS.gene066676.t1 MTR_1g047720 76.282 156 37 0 12 167 64 219 1.09e-91 266
MS.gene066676.t1 MTR_7g058900 84.615 156 24 0 12 167 64 219 9.80e-84 246
MS.gene066676.t1 MTR_2g436870 71.795 156 44 0 12 167 64 219 2.58e-82 242
MS.gene066676.t1 MTR_4g083490 80.303 132 26 0 12 143 66 197 1.21e-77 230
MS.gene066676.t1 MTR_1g047710 78.125 128 28 0 9 136 9 136 5.67e-75 221
MS.gene066676.t1 MTR_2g028790 75.758 132 32 0 12 143 68 199 3.65e-74 221
MS.gene066676.t1 MTR_4g022470 92.035 113 9 0 7 119 5 117 6.68e-73 214
MS.gene066676.t1 MTR_7g058640 75.758 132 32 0 12 143 64 195 1.82e-71 214
MS.gene066676.t1 MTR_7g058640 77.778 117 26 0 27 143 1 117 1.09e-64 194
MS.gene066676.t1 MTR_1g111890 52.874 174 63 1 13 167 65 238 3.35e-63 194
MS.gene066676.t1 MTR_7g058640 68.254 126 38 1 12 137 64 187 8.01e-58 179
MS.gene066676.t1 MTR_4g022550 96.825 63 2 0 105 167 9 71 5.81e-39 127
MS.gene066676.t1 MTR_5g089370 40.667 150 85 3 16 164 66 212 1.54e-33 118
MS.gene066676.t1 MTR_4g115390 34.228 149 96 2 16 164 70 216 1.66e-30 110
MS.gene066676.t1 MTR_4g052550 33.548 155 101 2 12 166 62 214 9.15e-28 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066676.t1 AT1G04760 78.846 156 33 0 12 167 64 219 1.04e-86 253
MS.gene066676.t1 AT1G04760 78.846 156 33 0 12 167 64 219 1.04e-86 253
MS.gene066676.t1 AT1G04750 83.974 156 25 0 12 167 64 219 9.34e-85 248
MS.gene066676.t1 AT2G33120 80.864 162 31 0 6 167 66 227 2.43e-84 248
MS.gene066676.t1 AT2G32670 82.692 156 27 0 12 167 129 284 4.34e-84 249
MS.gene066676.t1 AT2G33120 83.333 156 26 0 12 167 64 219 8.16e-84 246
MS.gene066676.t1 AT1G04750 84.397 141 22 0 27 167 1 141 5.15e-75 221
MS.gene066676.t1 AT1G04750 84.397 141 22 0 27 167 1 141 5.15e-75 221
MS.gene066676.t1 AT1G04750 84.397 141 22 0 27 167 1 141 5.15e-75 221
MS.gene066676.t1 AT3G54300 52.874 174 63 2 13 167 65 238 3.67e-61 189
MS.gene066676.t1 AT3G54300 52.874 174 63 2 13 167 65 238 3.67e-61 189
MS.gene066676.t1 AT3G54300 52.874 174 63 2 13 167 65 238 3.67e-61 189
MS.gene066676.t1 AT2G33110 66.026 156 49 1 12 167 64 215 6.31e-57 178
MS.gene066676.t1 AT4G15780 69.565 115 35 0 12 126 65 179 1.98e-55 173
MS.gene066676.t1 AT2G33110 67.677 99 32 0 12 110 64 162 6.47e-45 146
MS.gene066676.t1 AT5G11150 43.791 153 80 5 16 166 67 215 2.10e-37 128
MS.gene066676.t1 AT5G11150 43.791 153 80 5 16 166 67 215 2.67e-37 128
MS.gene066676.t1 AT2G33120 83.696 92 15 0 76 167 46 137 9.66e-37 124
MS.gene066676.t1 AT5G22360 37.419 155 95 2 12 166 63 215 4.39e-35 122
MS.gene066676.t1 AT4G32150 39.597 149 88 2 16 164 66 212 8.36e-33 116
MS.gene066676.t1 AT2G25340 37.333 150 90 3 16 164 66 212 5.37e-28 103
MS.gene066676.t1 AT3G24890 53.191 94 38 2 61 148 14 107 1.61e-25 95.1
MS.gene066676.t1 AT3G24890 54.839 62 24 1 61 122 14 71 1.59e-15 67.8
MS.gene066676.t1 AT3G24890 54.839 62 24 1 61 122 14 71 1.59e-15 67.8
MS.gene066676.t1 AT3G24890 54.839 62 24 1 61 122 14 71 1.59e-15 67.8
MS.gene066676.t1 AT3G24890 54.839 62 24 1 61 122 14 71 1.59e-15 67.8

Find 32 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTCTTAACAAAGAATT+TGG 0.112791 4.2:+80328985 MS.gene066676:CDS
GCTCAAGTTTCTGAAGTTAA+AGG 0.200850 4.2:+80329166 MS.gene066676:CDS
AGCATTTCTTGAACGTATTA+AGG 0.224570 4.2:+80328912 MS.gene066676:CDS
GGGAGAGAAGATTGAGGTTT+TGG 0.250137 4.2:+80329445 MS.gene066676:CDS
ACCACATCTTTCTCCTCAAT+TGG 0.292197 4.2:-80330110 None:intergenic
GTTATGTGCAGGTTATTGAT+AGG 0.320869 4.2:+80329423 MS.gene066676:intron
TGCATTTGCTCCTTCAACTT+TGG 0.326828 4.2:-80329094 None:intergenic
ATACGTTCAAGAAATGCTAT+TGG 0.334209 4.2:-80328907 None:intergenic
ATTTGCAGGCACAAGATTTC+AGG 0.345983 4.2:+80330076 MS.gene066676:intron
GCAAGTTTGCTAACTTCCTC+TGG 0.381827 4.2:-80329133 None:intergenic
TGAAGTTAAAGGAGTTATGA+TGG 0.406422 4.2:+80329177 MS.gene066676:CDS
AACTGAGAACCTTCGCTCTC+AGG 0.417900 4.2:+80329475 MS.gene066676:CDS
CCACATCTTTCTCCTCAATT+GGG 0.430467 4.2:-80330109 None:intergenic
GGCACAAGATTTCAGGCAGC+AGG 0.435566 4.2:+80330083 MS.gene066676:intron
TTATGTGCAGGTTATTGATA+GGG 0.472672 4.2:+80329424 MS.gene066676:intron
AGCTTACTGTGTTGTGGCAG+TGG 0.485476 4.2:+80328864 MS.gene066676:CDS
ATTGTTCTTTCTGTGTGCCA+TGG 0.487473 4.2:+80330192 MS.gene066676:CDS
AAAGTCTTAACAAAGAATTT+GGG 0.498770 4.2:+80328986 MS.gene066676:CDS
TGATAGGGGAGAGAAGATTG+AGG 0.506422 4.2:+80329439 MS.gene066676:CDS
ATGCATTAGACCAAAGTTGA+AGG 0.514320 4.2:+80329084 MS.gene066676:intron
TATGTGCAGGTTATTGATAG+GGG 0.533979 4.2:+80329425 MS.gene066676:intron
TTTAATAAAAGATATGGTGG+AGG 0.542291 4.2:+80328940 MS.gene066676:CDS
GGCAGCAGGGAACCCAATTG+AGG 0.564168 4.2:+80330097 MS.gene066676:CDS
ATAGTTAACAACTGAAGCCA+TGG 0.593585 4.2:-80330209 None:intergenic
AGAATCTAACCTGAGAGCGA+AGG 0.618405 4.2:-80329484 None:intergenic
GCACAAGATTTCAGGCAGCA+GGG 0.625497 4.2:+80330084 MS.gene066676:CDS
GTTGTGGCAGTGGAGTCTGT+TGG 0.632770 4.2:+80328874 MS.gene066676:CDS
CCCAATTGAGGAGAAAGATG+TGG 0.650037 4.2:+80330109 MS.gene066676:CDS
CATGGAAGACATGAATGCCA+AGG 0.663385 4.2:+80327517 None:intergenic
ATATTGTGTGGAACATCCAG+AGG 0.668552 4.2:+80329117 MS.gene066676:CDS
GGAGCAAATGCAATATTGTG+TGG 0.673674 4.2:+80329105 MS.gene066676:CDS
TATGATGGAAAATATCGACA+AGG 0.735916 4.2:+80329192 MS.gene066676:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTTTTTTTTTTTTTT+TGG + chr4.2:80328771-80328790 MS.gene066676:intron 0.0%
!! TAAAAATAAAATATGAGTTT+AGG + chr4.2:80328529-80328548 MS.gene066676:intron 10.0%
!! AAAATCATAAATTTGAAGAT+AGG - chr4.2:80328713-80328732 None:intergenic 15.0%
!! AAATCATAAATTTGAAGATA+GGG - chr4.2:80328712-80328731 None:intergenic 15.0%
!! ATAAAATCTTGATACTTTAA+TGG + chr4.2:80329926-80329945 MS.gene066676:intron 15.0%
!! TAAAATCTTGATACTTTAAT+GGG + chr4.2:80329927-80329946 MS.gene066676:intron 15.0%
!! TAGATTTAATTAATGCTTAA+TGG + chr4.2:80328450-80328469 MS.gene066676:intron 15.0%
!!! ATCCAAAACTTTAAAATATT+GGG - chr4.2:80329705-80329724 None:intergenic 15.0%
!!! GATGATTTTAATAAAAGATA+TGG + chr4.2:80328934-80328953 MS.gene066676:CDS 15.0%
!! AAAAGTCTTAACAAAGAATT+TGG + chr4.2:80328985-80329004 MS.gene066676:CDS 20.0%
!! AAAGTCTTAACAAAGAATTT+GGG + chr4.2:80328986-80329005 MS.gene066676:CDS 20.0%
!! ATCAAAGAAACTTGAAAATT+TGG + chr4.2:80327592-80327611 MS.gene066676:intron 20.0%
!! CATACAATATTCACAATTAA+TGG - chr4.2:80330016-80330035 None:intergenic 20.0%
!! TATTATTATAAGTTATGTGC+AGG + chr4.2:80329412-80329431 MS.gene066676:intron 20.0%
!! TTAATTGTGAATATTGTATG+AGG + chr4.2:80330016-80330035 MS.gene066676:intron 20.0%
!!! ATCCCAATATTTTAAAGTTT+TGG + chr4.2:80329700-80329719 MS.gene066676:intron 20.0%
!!! CAATATTTTAAAGTTTTGGA+TGG + chr4.2:80329704-80329723 MS.gene066676:intron 20.0%
!!! CATCCAAAACTTTAAAATAT+TGG - chr4.2:80329706-80329725 None:intergenic 20.0%
!!! GATTTTAATAAAAGATATGG+TGG + chr4.2:80328937-80328956 MS.gene066676:CDS 20.0%
!!! TTTTATGAAACTGGAATTAT+TGG - chr4.2:80329912-80329931 None:intergenic 20.0%
! AAGAAACTTGAAAATTTGGA+TGG + chr4.2:80327596-80327615 MS.gene066676:intron 25.0%
! TAATCATGAAACATGTTCAA+CGG - chr4.2:80328832-80328851 None:intergenic 25.0%
! TTTAATAAAAGATATGGTGG+AGG + chr4.2:80328940-80328959 MS.gene066676:CDS 25.0%
!! AGCTTCAAATGGAAAATTTT+TGG - chr4.2:80327939-80327958 None:intergenic 25.0%
!! GTATCAAGATTTTATGAAAC+TGG - chr4.2:80329921-80329940 None:intergenic 25.0%
!!! CAGTCAAGTTTGTTTTATTA+TGG - chr4.2:80327965-80327984 None:intergenic 25.0%
!!! TTTTAATTGTGATGATGAAG+TGG + chr4.2:80328308-80328327 MS.gene066676:intron 25.0%
AAACATGTTCAACGGTAAAA+AGG - chr4.2:80328824-80328843 None:intergenic 30.0%
ACAAAAAAAGGATGGCATAT+AGG + chr4.2:80327649-80327668 MS.gene066676:intron 30.0%
AGCATTTCTTGAACGTATTA+AGG + chr4.2:80328912-80328931 MS.gene066676:CDS 30.0%
ATACGTTCAAGAAATGCTAT+TGG - chr4.2:80328910-80328929 None:intergenic 30.0%
ATGAATTAATTGTCAGCAGT+TGG + chr4.2:80328490-80328509 MS.gene066676:intron 30.0%
ATTATAGTGTCCAAATCTCT+TGG + chr4.2:80329728-80329747 MS.gene066676:intron 30.0%
CATAAATTTGAAGATAGGGA+GGG - chr4.2:80328708-80328727 None:intergenic 30.0%
CTTCTATTCATTGTTCATGT+AGG - chr4.2:80328139-80328158 None:intergenic 30.0%
GAGGGTAAGTGTTAAAATTA+TGG - chr4.2:80328690-80328709 None:intergenic 30.0%
GAGTTTCATTTAATATCACG+TGG + chr4.2:80329379-80329398 MS.gene066676:intron 30.0%
GATTGGAATGTCAATTGAAA+TGG + chr4.2:80330048-80330067 MS.gene066676:intron 30.0%
GGATTCGACTAAACAAAAAA+AGG + chr4.2:80327637-80327656 MS.gene066676:intron 30.0%
GTGATTCTTACTTGCTTAAA+TGG + chr4.2:80328655-80328674 MS.gene066676:intron 30.0%
GTTTGGTATCTATATGTCAT+TGG + chr4.2:80329297-80329316 MS.gene066676:intron 30.0%
TCATAAATTTGAAGATAGGG+AGG - chr4.2:80328709-80328728 None:intergenic 30.0%
TCGACTAAACAAAAAAAGGA+TGG + chr4.2:80327641-80327660 MS.gene066676:intron 30.0%
TGAACATGTTTCATGATTAG+TGG + chr4.2:80328833-80328852 MS.gene066676:intron 30.0%
TGAAGTTAAAGGAGTTATGA+TGG + chr4.2:80329177-80329196 MS.gene066676:CDS 30.0%
TGACACTTCATATTGAAGAA+CGG + chr4.2:80329275-80329294 MS.gene066676:intron 30.0%
TTACTTGCTTAAATGGCTTA+TGG + chr4.2:80328662-80328681 MS.gene066676:intron 30.0%
TTATGTGCAGGTTATTGATA+GGG + chr4.2:80329424-80329443 MS.gene066676:intron 30.0%
TTATGTGTTTGTGTATGTCT+CGG + chr4.2:80329329-80329348 MS.gene066676:intron 30.0%
TTGAAATGGTATTCATTTGC+AGG + chr4.2:80330062-80330081 MS.gene066676:intron 30.0%
TTGATGAATACAGTCCTATT+GGG + chr4.2:80327803-80327822 MS.gene066676:intron 30.0%
TTGGAACTCAAATATCTTCT+CGG - chr4.2:80327842-80327861 None:intergenic 30.0%
TTTGATGAATACAGTCCTAT+TGG + chr4.2:80327802-80327821 MS.gene066676:intron 30.0%
! AGGAAATTTTCAGTTGATCT+TGG - chr4.2:80328119-80328138 None:intergenic 30.0%
! TATGATGGAAAATATCGACA+AGG + chr4.2:80329192-80329211 MS.gene066676:CDS 30.0%
! TTGTTATTCCACCTAGTATT+AGG - chr4.2:80328414-80328433 None:intergenic 30.0%
!! AAGGCTCTTGTTTGATATTT+TGG - chr4.2:80327861-80327880 None:intergenic 30.0%
!! AAGTGTCAGTGCTATTTATT+TGG - chr4.2:80329263-80329282 None:intergenic 30.0%
!! ATGGTATTTTGAGCAATGTA+TGG + chr4.2:80328370-80328389 MS.gene066676:intron 30.0%
!! TGGTATTTTGAGCAATGTAT+GGG + chr4.2:80328371-80328390 MS.gene066676:intron 30.0%
!!! GATGTTGGTTTTACAAAAAC+AGG + chr4.2:80328200-80328219 MS.gene066676:intron 30.0%
AATCTCACATTAGATCCTTG+TGG - chr4.2:80327561-80327580 None:intergenic 35.0%
ACTCACACTTGAAACAAACA+AGG - chr4.2:80329615-80329634 None:intergenic 35.0%
AGGACTGTATTCATCAAAGT+TGG - chr4.2:80327800-80327819 None:intergenic 35.0%
AGTTGATCTTGGTCGATTAT+GGG - chr4.2:80328108-80328127 None:intergenic 35.0%
ATGCATTAGACCAAAGTTGA+AGG + chr4.2:80329084-80329103 MS.gene066676:intron 35.0%
ATGGAAGACAGTGTTATCAA+TGG + chr4.2:80327888-80327907 MS.gene066676:intron 35.0%
CACCACTAACAGAATTATCA+TGG + chr4.2:80328009-80328028 MS.gene066676:intron 35.0%
CTCCATGATAATTCTGTTAG+TGG - chr4.2:80328014-80328033 None:intergenic 35.0%
CTTCATATTGAAGAACGGTT+TGG + chr4.2:80329280-80329299 MS.gene066676:intron 35.0%
GAAACTGGAATTATTGGACT+TGG - chr4.2:80329906-80329925 None:intergenic 35.0%
GGACTGTATTCATCAAAGTT+GGG - chr4.2:80327799-80327818 None:intergenic 35.0%
GTATGAGGATTGTGAATGAT+TGG + chr4.2:80330031-80330050 MS.gene066676:intron 35.0%
GTTATGTGCAGGTTATTGAT+AGG + chr4.2:80329423-80329442 MS.gene066676:intron 35.0%
TATGTGCAGGTTATTGATAG+GGG + chr4.2:80329425-80329444 MS.gene066676:intron 35.0%
TATTATGGCAGAGCTTCAAA+TGG - chr4.2:80327950-80327969 None:intergenic 35.0%
TCATTCGAAACTTGTCTTCA+AGG - chr4.2:80327741-80327760 None:intergenic 35.0%
TCGAATGAATGAGTTTGAGA+AGG + chr4.2:80327753-80327772 MS.gene066676:intron 35.0%
TGAGATAGTTACTTGGATGT+TGG + chr4.2:80328185-80328204 MS.gene066676:intron 35.0%
TGGAAGACAGTGTTATCAAT+GGG + chr4.2:80327889-80327908 MS.gene066676:intron 35.0%
TGGCATTCTTTGATTGTTGA+AGG + chr4.2:80327616-80327635 MS.gene066676:intron 35.0%
TTAGATCCTTGTGGTAGAAT+TGG - chr4.2:80327552-80327571 None:intergenic 35.0%
TTCCAAACTTGCCTAATACT+AGG + chr4.2:80328400-80328419 MS.gene066676:intron 35.0%
! CAACATCTGAGATAGTTACT+TGG + chr4.2:80328178-80328197 MS.gene066676:intron 35.0%
! TTGTTTGTTTCAAGTGTGAG+TGG + chr4.2:80329614-80329633 MS.gene066676:intron 35.0%
!! GCTCAAGTTTCTGAAGTTAA+AGG + chr4.2:80329166-80329185 MS.gene066676:CDS 35.0%
!!! AAATTTTTGGTTTTCAGCCG+TGG - chr4.2:80327926-80327945 None:intergenic 35.0%
ACAGTTCCAATTCTACCACA+AGG + chr4.2:80327543-80327562 MS.gene066676:intron 40.0%
ACCACATCTTTCTCCTCAAT+TGG - chr4.2:80330113-80330132 None:intergenic 40.0%
AGAATTGGAACTGTGTACCT+TGG - chr4.2:80327537-80327556 None:intergenic 40.0%
ATATTGTGTGGAACATCCAG+AGG + chr4.2:80329117-80329136 MS.gene066676:CDS 40.0%
ATCTTGGTCGATTATGGGTT+AGG - chr4.2:80328103-80328122 None:intergenic 40.0%
ATTGTTCTTTCTGTGTGCCA+TGG + chr4.2:80330192-80330211 MS.gene066676:CDS 40.0%
ATTTGCAGGCACAAGATTTC+AGG + chr4.2:80330076-80330095 MS.gene066676:intron 40.0%
CAAACTTGCCTAATACTAGG+TGG + chr4.2:80328403-80328422 MS.gene066676:intron 40.0%
CACCTAGTATTAGGCAAGTT+TGG - chr4.2:80328405-80328424 None:intergenic 40.0%
CAGAGAATGAAACACTCACA+AGG - chr4.2:80328040-80328059 None:intergenic 40.0%
CAGTTGATCTTGGTCGATTA+TGG - chr4.2:80328109-80328128 None:intergenic 40.0%
CCACATCTTTCTCCTCAATT+GGG - chr4.2:80330112-80330131 None:intergenic 40.0%
GAAGACAGTGTTATCAATGG+GGG + chr4.2:80327891-80327910 MS.gene066676:intron 40.0%
GGAAGACAGTGTTATCAATG+GGG + chr4.2:80327890-80327909 MS.gene066676:intron 40.0%
GGAGCAAATGCAATATTGTG+TGG + chr4.2:80329105-80329124 MS.gene066676:CDS 40.0%
TAAGAGCAAGCCAATGATTC+AGG - chr4.2:80329758-80329777 None:intergenic 40.0%
TAATTGTCAGCAGTTGGCTA+AGG + chr4.2:80328496-80328515 MS.gene066676:intron 40.0%
TGCATTTGCTCCTTCAACTT+TGG - chr4.2:80329097-80329116 None:intergenic 40.0%
TTAATGCCACCATCCAACAA+TGG - chr4.2:80328345-80328364 None:intergenic 40.0%
TTGATGCCTTAGAGATCACT+GGG + chr4.2:80329801-80329820 MS.gene066676:intron 40.0%
! TTTTGCAGCTTACTGTGTTG+TGG + chr4.2:80328858-80328877 MS.gene066676:CDS 40.0%
!! AGAGAAGATTGAGGTTTTGG+TGG + chr4.2:80329448-80329467 MS.gene066676:CDS 40.0%
AACAAGAGCCTTCGAATGCA+TGG + chr4.2:80327869-80327888 MS.gene066676:intron 45.0%
AATATCTTCTCGGCCTTAGC+TGG - chr4.2:80327832-80327851 None:intergenic 45.0%
ACAGTGTTATCAATGGGGGA+TGG + chr4.2:80327895-80327914 MS.gene066676:intron 45.0%
ACTGTCTTCCATGCATTCGA+AGG - chr4.2:80327880-80327899 None:intergenic 45.0%
AGAATCTAACCTGAGAGCGA+AGG - chr4.2:80329487-80329506 None:intergenic 45.0%
CCCAATTGAGGAGAAAGATG+TGG + chr4.2:80330109-80330128 MS.gene066676:CDS 45.0%
GTTGATGCCTTAGAGATCAC+TGG + chr4.2:80329800-80329819 MS.gene066676:intron 45.0%
TCGAATCCCAGTGATCTCTA+AGG - chr4.2:80329810-80329829 None:intergenic 45.0%
TCTTGGTCGTCCTGAATCAT+TGG + chr4.2:80329745-80329764 MS.gene066676:intron 45.0%
TGATAGGGGAGAGAAGATTG+AGG + chr4.2:80329439-80329458 MS.gene066676:CDS 45.0%
TTCAGGACGACCAAGAGATT+TGG - chr4.2:80329741-80329760 None:intergenic 45.0%
! GATGGTGGCATTAATCGTGA+TGG + chr4.2:80328351-80328370 MS.gene066676:intron 45.0%
! GGGAGAGAAGATTGAGGTTT+TGG + chr4.2:80329445-80329464 MS.gene066676:CDS 45.0%
!! GCAAGTTTGCTAACTTCCTC+TGG - chr4.2:80329136-80329155 None:intergenic 45.0%
AACTGAGAACCTTCGCTCTC+AGG + chr4.2:80329475-80329494 MS.gene066676:CDS 50.0%
AGCTTACTGTGTTGTGGCAG+TGG + chr4.2:80328864-80328883 MS.gene066676:CDS 50.0%
CATCCAACAATGGAAGCGGA+CGG - chr4.2:80328335-80328354 None:intergenic 50.0%
CCACCATCCAACAATGGAAG+CGG - chr4.2:80328339-80328358 None:intergenic 50.0%
GCACAAGATTTCAGGCAGCA+GGG + chr4.2:80330084-80330103 MS.gene066676:CDS 50.0%
TCCTATTGGGTGACCAGCTA+AGG + chr4.2:80327816-80327835 MS.gene066676:intron 50.0%
CCGCTTCCATTGTTGGATGG+TGG + chr4.2:80328336-80328355 MS.gene066676:intron 55.0%
CGTCCGCTTCCATTGTTGGA+TGG + chr4.2:80328333-80328352 MS.gene066676:intron 55.0%
GCCTTAGCTGGTCACCCAAT+AGG - chr4.2:80327820-80327839 None:intergenic 55.0%
GGCACAAGATTTCAGGCAGC+AGG + chr4.2:80330083-80330102 MS.gene066676:intron 55.0%
GTTGTGGCAGTGGAGTCTGT+TGG + chr4.2:80328874-80328893 MS.gene066676:CDS 55.0%
GGACCGTCCGCTTCCATTGT+TGG + chr4.2:80328329-80328348 MS.gene066676:intron 60.0%
GGCAGCAGGGAACCCAATTG+AGG + chr4.2:80330097-80330116 MS.gene066676:CDS 60.0%
AATGGGGGATGGCTGCGCCA+CGG + chr4.2:80327906-80327925 MS.gene066676:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 80327518 80330227 80327518 ID=MS.gene066676
chr4.2 mRNA 80327518 80330227 80327518 ID=MS.gene066676.t1;Parent=MS.gene066676
chr4.2 exon 80327518 80327538 80327518 ID=MS.gene066676.t1.exon1;Parent=MS.gene066676.t1
chr4.2 CDS 80327518 80327538 80327518 ID=cds.MS.gene066676.t1;Parent=MS.gene066676.t1
chr4.2 exon 80328853 80329007 80328853 ID=MS.gene066676.t1.exon2;Parent=MS.gene066676.t1
chr4.2 CDS 80328853 80329007 80328853 ID=cds.MS.gene066676.t1;Parent=MS.gene066676.t1
chr4.2 exon 80329093 80329213 80329093 ID=MS.gene066676.t1.exon3;Parent=MS.gene066676.t1
chr4.2 CDS 80329093 80329213 80329093 ID=cds.MS.gene066676.t1;Parent=MS.gene066676.t1
chr4.2 exon 80329434 80329496 80329434 ID=MS.gene066676.t1.exon4;Parent=MS.gene066676.t1
chr4.2 CDS 80329434 80329496 80329434 ID=cds.MS.gene066676.t1;Parent=MS.gene066676.t1
chr4.2 exon 80330084 80330227 80330084 ID=MS.gene066676.t1.exon5;Parent=MS.gene066676.t1
chr4.2 CDS 80330084 80330227 80330084 ID=cds.MS.gene066676.t1;Parent=MS.gene066676.t1
Gene Sequence

>MS.gene066676

ATGGAAGACATGAATGCCAAGGTACACAGTTCCAATTCTACCACAAGGATCTAATGTGAGATTTTGAATAACAGATCAAAGAAACTTGAAAATTTGGATGGCATTCTTTGATTGTTGAAGGATTCGACTAAACAAAAAAAGGATGGCATATAGGAATTTCTAACAGTGTTATAAATAATTAAACGTATGATCATAGACATTTTGATGTTTATTCAATGAACCTTGAAGACAAGTTTCGAATGAATGAGTTTGAGAAGGTTTTTTAGAAAGTTGAATTTCCCAACTTTGATGAATACAGTCCTATTGGGTGACCAGCTAAGGCCGAGAAGATATTTGAGTTCCAAAATATCAAACAAGAGCCTTCGAATGCATGGAAGACAGTGTTATCAATGGGGGATGGCTGCGCCACGGCTGAAAACCAAAAATTTTCCATTTGAAGCTCTGCCATAATAAAACAAACTTGACTGCAATTTGATTATGTCATCTTCACGCACCACTAACAGAATTATCATGGAGAAGCCTTGTGAGTGTTTCATTCTCTGTGAACATTGAATATAAATACTTTTCATTGATATAATATATCCTAACCCATAATCGACCAAGATCAACTGAAAATTTCCTACATGAACAATGAATAGAAGTTTAAGAATCTTCTTGTTGCAACATCTGAGATAGTTACTTGGATGTTGGTTTTACAAAAACAGGAAAAAAAAATATTTACAAGTCACAAAACTCAATTTATATTTGTTTTTTTCTTATTTTTGTCAATGTGATATTAATCATGTTACTATTTTAATTGTGATGATGAAGTGGACCGTCCGCTTCCATTGTTGGATGGTGGCATTAATCGTGATGGTATTTTGAGCAATGTATGGGTCAATGTTCCAAACTTGCCTAATACTAGGTGGAATAACAACTAGTCGATGTTATTATAGATTTAATTAATGCTTAATGGTGTGTTATTTTTTTAAAATGAATTAATTGTCAGCAGTTGGCTAAGGACAGCTTGTTTAAAAATAAAATATGAGTTTAGGAGATTTTAGTTGATGCTATTATAATCAAATCTTACATATTGTTATTAAATTATAATTATTTCTAAAGAGTGAAATTTTGTTTGTTATAATAATACAATATGTAGTGATTCTTACTTGCTTAAATGGCTTATGGTGCCATAATTTTAACACTTACCCTCCCTATCTTCAAATTTATGATTTTAATTTGTTCATCTATATATATATATATATATATATATATTTTTTTTTTTTTTTTTTTTTTTGGCTCATTAGATCATCTATATTGTGTTCCTTTTTACCGTTGAACATGTTTCATGATTAGTGGAGTTTTGCAGCTTACTGTGTTGTGGCAGTGGAGTCTGTTGGTAGACAAATTCCAATAGCATTTCTTGAACGTATTAAGGATGATTTTAATAAAAGATATGGTGGAGGAAGAGCTACAACAGCAACTGCTAAAAGTCTTAACAAAGAATTTGGGTACTTAATTATGTTTCTTATCTTCTTGTGCAACATATATTTACATGTTAATTACTCTATAATATTTATTATATATATGCATTAGACCAAAGTTGAAGGAGCAAATGCAATATTGTGTGGAACATCCAGAGGAAGTTAGCAAACTTGCTAAAGTGAAAGCTCAAGTTTCTGAAGTTAAAGGAGTTATGATGGAAAATATCGACAAGGTATTGCATTGATTGCTGCACATACTAATACTTACACTATGTCTGTCCAAATAAATAGCACTGACACTTCATATTGAAGAACGGTTTGGTATCTATATGTCATTGGCATATCGTGTTATGTGTTTGTGTATGTCTCGGTGCTTCATAGCTATCAAGTTGTCTCTAGAGTTTCATTTAATATCACGTGGCAAGTTATAATATTATTATAAGTTATGTGCAGGTTATTGATAGGGGAGAGAAGATTGAGGTTTTGGTGGATAAAACTGAGAACCTTCGCTCTCAGGTTAGATTCTTAATAGTTTTATATATGTAATTTGAATTAGAGATGAGTATTGAGTAGATTTTGTATTAATCTCATGCATATAGTATTTAATTCTTAAATTGAACTTGAATATCGTCCTTGTTTGTTTCAAGTGTGAGTGGTTAGTTTACATCGACTATGAACAAGTCAATGTTATATATAAGACATGTGATTTGTATGATTGTATCCCAATATTTTAAAGTTTTGGATGGAATTATAGTGTCCAAATCTCTTGGTCGTCCTGAATCATTGGCTTGCTCTTAAATTCTGACATCTAGTTTTTTTGTTGATGCCTTAGAGATCACTGGGATTCGAGCTTAGCTTGTTTTGTTGTGATTCATTCGTTTCATTTTTGATAAAAATTACTTTTCTGTTATATCAGATGCAACCAAGTCCAATAATTCCAGTTTCATAAAATCTTGATACTTTAATGGGTTGATTTGTTATCAGTTTATTTATTATATATGCTAAAACTTTGCTTTATGCTACTTAATTTTTCCATTAATTGTGAATATTGTATGAGGATTGTGAATGATTGGAATGTCAATTGAAATGGTATTCATTTGCAGGCACAAGATTTCAGGCAGCAGGGAACCCAATTGAGGAGAAAGATGTGGTATCAAAACATGAAGATCAAGCTCATAGTTCTTGCTATCATAATTGCATTGATTCTCATTATTGTTCTTTCTGTGTGCCATGGCTTCAGTTGTTAA
Protein sequence

>MS.gene066676.t1

MEDMNAKWSFAAYCVVAVESVGRQIPIAFLERIKDDFNKRYGGGRATTATAKSLNKEFGPKLKEQMQYCVEHPEEVSKLAKVKAQVSEVKGVMMENIDKVIDRGEKIEVLVDKTENLRSQAQDFRQQGTQLRRKMWYQNMKIKLIVLAIIIALILIIVLSVCHGFSC