AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066724

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066724.t1 MTR_4g091200 92.553 470 35 0 1 470 59 528 0.0 892
MS.gene066724.t1 MTR_6g017205 91.453 468 40 0 1 468 59 526 0.0 870
MS.gene066724.t1 MTR_4g057990 82.303 469 79 2 1 469 59 523 0.0 780
MS.gene066724.t1 MTR_4g091190 80.342 468 90 1 1 468 63 528 0.0 767
MS.gene066724.t1 MTR_4g088355 78.678 469 100 0 1 469 66 534 0.0 758
MS.gene066724.t1 MTR_2g023590 80.213 470 90 2 1 469 1 468 0.0 754
MS.gene066724.t1 MTR_4g091170 73.203 459 105 1 10 468 102 542 0.0 707
MS.gene066724.t1 MTR_4g091150 63.675 468 168 2 1 466 71 538 0.0 599
MS.gene066724.t1 MTR_2g031560 54.292 466 210 2 3 467 59 522 0.0 542
MS.gene066724.t1 MTR_2g031590 53.715 471 209 4 3 467 64 531 3.85e-180 515
MS.gene066724.t1 MTR_4g094498 53.763 465 211 3 3 467 73 533 1.70e-173 499
MS.gene066724.t1 MTR_2g031390 50.321 467 224 6 4 466 65 527 6.07e-166 479
MS.gene066724.t1 MTR_4g094488 53.700 473 210 4 3 468 67 537 1.20e-164 476
MS.gene066724.t1 MTR_4g094495 52.331 472 216 4 4 468 71 540 1.48e-163 473
MS.gene066724.t1 MTR_4g094492 50.959 469 224 3 4 467 68 535 6.21e-163 471
MS.gene066724.t1 MTR_4g094520 51.812 469 215 6 4 467 69 531 9.89e-163 471
MS.gene066724.t1 MTR_3g009820 48.922 464 230 5 4 466 63 520 6.40e-162 468
MS.gene066724.t1 MTR_4g094480 51.599 469 222 3 4 468 68 535 9.87e-161 466
MS.gene066724.t1 MTR_4g094478 52.350 468 218 3 4 467 68 534 1.80e-160 465
MS.gene066724.t1 MTR_4g094518 50.533 469 218 7 5 466 67 528 1.82e-159 462
MS.gene066724.t1 MTR_2g031490 51.489 470 218 6 3 466 71 536 5.06e-159 461
MS.gene066724.t1 MTR_2g031510 50.105 475 217 6 3 466 73 538 1.96e-156 455
MS.gene066724.t1 MTR_3g009880 48.922 464 230 5 4 466 61 518 6.71e-155 451
MS.gene066724.t1 MTR_3g009760 47.629 464 236 5 4 466 63 520 1.88e-154 449
MS.gene066724.t1 MTR_2g031420 50.855 468 223 4 3 469 67 528 1.99e-153 447
MS.gene066724.t1 MTR_4g094418 51.259 437 205 4 40 469 1 436 5.89e-153 448
MS.gene066724.t1 MTR_2g031460 50.000 464 225 4 3 465 67 524 1.44e-152 445
MS.gene066724.t1 MTR_4g094515 51.380 471 220 6 3 468 74 540 2.52e-149 455
MS.gene066724.t1 MTR_4g094515 51.496 468 220 5 3 467 686 1149 1.91e-148 452
MS.gene066724.t1 MTR_1g076830 38.559 472 274 8 3 465 68 532 2.75e-101 313
MS.gene066724.t1 MTR_2g031600 65.000 180 63 0 3 182 65 244 1.01e-71 233
MS.gene066724.t1 MTR_2g031600 51.875 160 76 1 308 467 241 399 1.54e-53 185
MS.gene066724.t1 MTR_3g009850 48.214 224 113 3 244 466 5 226 1.28e-64 208
MS.gene066724.t1 MTR_2g084370 46.847 111 37 2 64 174 1 89 5.49e-29 110
MS.gene066724.t1 MTR_2g084405 45.570 79 43 0 64 142 1 79 3.86e-16 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066724.t1 AT4G20800 55.365 466 205 2 4 469 64 526 5.63e-178 509
MS.gene066724.t1 AT1G26420 55.172 464 202 4 4 466 65 523 1.88e-176 505
MS.gene066724.t1 AT1G26380 55.388 464 201 4 4 466 65 523 7.70e-175 501
MS.gene066724.t1 AT1G26390 55.914 465 199 4 4 466 65 525 8.64e-175 501
MS.gene066724.t1 AT1G30720 53.419 468 213 3 2 468 63 526 2.38e-172 495
MS.gene066724.t1 AT1G34575 54.915 468 197 6 3 466 65 522 6.11e-171 491
MS.gene066724.t1 AT1G26400 53.632 468 211 6 4 470 65 527 1.49e-170 490
MS.gene066724.t1 AT1G30730 52.137 468 219 3 2 468 62 525 1.22e-169 488
MS.gene066724.t1 AT1G26410 55.172 464 202 5 4 466 89 547 6.02e-169 487
MS.gene066724.t1 AT1G30710 54.025 472 202 7 3 469 68 529 1.86e-168 485
MS.gene066724.t1 AT1G30700 52.350 468 214 5 3 466 62 524 3.88e-165 476
MS.gene066724.t1 AT5G44400 50.107 467 225 6 4 466 72 534 1.55e-159 462
MS.gene066724.t1 AT5G44390 50.746 469 223 5 4 467 74 539 4.87e-158 459
MS.gene066724.t1 AT2G34790 50.000 468 224 5 4 468 70 530 2.84e-157 457
MS.gene066724.t1 AT2G34810 49.787 470 230 3 3 467 70 538 6.34e-157 456
MS.gene066724.t1 AT4G20840 51.064 470 217 7 3 467 73 534 3.21e-156 454
MS.gene066724.t1 AT1G30760 50.000 468 222 6 4 467 72 531 4.92e-154 448
MS.gene066724.t1 AT1G30760 50.000 468 222 6 4 467 90 549 5.40e-154 449
MS.gene066724.t1 AT1G11770 49.254 469 227 6 3 467 67 528 9.07e-151 440
MS.gene066724.t1 AT1G01980 50.947 475 215 9 3 467 67 533 1.01e-147 432
MS.gene066724.t1 AT5G44380 48.712 466 233 4 4 466 75 537 2.30e-146 429
MS.gene066724.t1 AT5G44380 48.712 466 233 4 4 466 115 577 3.73e-146 430
MS.gene066724.t1 AT4G20860 48.026 456 231 4 12 466 77 527 3.82e-146 428
MS.gene066724.t1 AT4G20820 48.069 466 228 5 3 466 71 524 1.07e-145 427
MS.gene066724.t1 AT5G44360 48.394 467 229 7 4 466 70 528 2.41e-142 419
MS.gene066724.t1 AT5G44360 48.394 467 229 7 4 466 86 544 4.13e-142 419
MS.gene066724.t1 AT4G20830 49.574 470 224 7 3 467 74 535 1.09e-141 417
MS.gene066724.t1 AT4G20830 49.574 470 224 7 3 467 74 535 4.66e-141 417
MS.gene066724.t1 AT5G44440 46.596 470 238 5 3 466 65 527 2.84e-139 410
MS.gene066724.t1 AT5G44440 46.596 470 238 5 3 466 135 597 6.28e-138 410
MS.gene066724.t1 AT5G44410 49.045 471 221 9 3 466 69 527 1.53e-136 404
MS.gene066724.t1 AT1G30740 47.660 470 235 7 3 467 67 530 3.14e-131 390
MS.gene066724.t1 AT1G30740 47.660 470 235 7 3 467 64 527 3.70e-131 390

Find 84 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCTAATGTTGTTCAAAT+TGG 0.228780 4.2:-21076290 MS.gene066724:CDS
AAGCTGAATGTTGTTGCTTT+TGG 0.230709 4.2:+21076862 None:intergenic
TTTGACATTGCTAAAACTTA+TGG 0.257856 4.2:-21075684 MS.gene066724:CDS
GGAAATTTGATGAGAAAATT+TGG 0.258916 4.2:-21076590 MS.gene066724:CDS
TGGCACATTTAACTGTTTCT+TGG 0.267359 4.2:+21076882 None:intergenic
AATAGATCTTTATGCAGTTT+TGG 0.273624 4.2:+21076323 None:intergenic
AATCATTAGACCACGAATTA+AGG 0.276835 4.2:+21075853 None:intergenic
AGTAAGGAATTCCCCGAATT+AGG 0.285169 4.2:-21076188 MS.gene066724:CDS
AACAAGCTTGCTTTCCCATC+TGG 0.299204 4.2:-21076662 MS.gene066724:CDS
ACTTTGGTTCAAACCTAATT+CGG 0.311377 4.2:+21076175 None:intergenic
TGACCAATTTGAACAACATT+AGG 0.312133 4.2:+21076287 None:intergenic
TCTTTCATCTTTCCACCATA+TGG 0.316468 4.2:+21075936 None:intergenic
ATAGATTCTAGAGCTTCTTT+AGG 0.318699 4.2:+21076005 None:intergenic
TCTAGAGCTTCTTTAGGAAT+AGG 0.321024 4.2:+21076011 None:intergenic
GGTGGTGGTGGTGCTAGTTT+TGG 0.331271 4.2:-21076467 MS.gene066724:CDS
AAGAAGCTCTAGAATCTATA+TGG 0.334429 4.2:-21076001 MS.gene066724:CDS
CTTTGGTTCAAACCTAATTC+GGG 0.343696 4.2:+21076176 None:intergenic
GGGAGGCATTCCACAATTAT+AGG 0.348238 4.2:-21075749 MS.gene066724:CDS
CATTTCTCTGGTGGTGGGTA+TGG 0.360817 4.2:-21076611 MS.gene066724:CDS
GTTATAATCGCCAAAGGTTT+CGG 0.368910 4.2:+21076926 None:intergenic
AATTATAGGGATGCTGATAT+TGG 0.378640 4.2:-21075735 MS.gene066724:CDS
GTAGTGCAAGTTAGTTTCAT+TGG 0.379275 4.2:-21076251 MS.gene066724:CDS
GTGATTGCCTTTCAATGCCT+TGG 0.391339 4.2:-21076154 MS.gene066724:CDS
TCAAATCTCGTTACATTACT+TGG 0.393941 4.2:+21075702 None:intergenic
ACGATAACCTGTGGAGTTAT+CGG 0.397449 4.2:+21076413 None:intergenic
TAAATTGGTTGAGGATCCTT+TGG 0.399190 4.2:+21076065 None:intergenic
TACTCCTCTTGAAGCATTGT+TGG 0.402290 4.2:-21076093 MS.gene066724:CDS
GGTGGTCATTTCTCTGGTGG+TGG 0.426644 4.2:-21076617 MS.gene066724:CDS
TCAACAGCATGGGTTGAATC+AGG 0.427451 4.2:-21076728 MS.gene066724:CDS
ACAAGCTTGCTTTCCCATCT+GGG 0.436569 4.2:-21076661 MS.gene066724:CDS
TCTTTCATGGAAACTTCAAT+TGG 0.441584 4.2:-21076438 MS.gene066724:CDS
GAAATTCTCAGGATCAACTT+TGG 0.444017 4.2:+21075614 None:intergenic
CTTATGCAATGGAATCCATA+TGG 0.450449 4.2:-21075951 MS.gene066724:CDS
GGAGGCATTCCACAATTATA+GGG 0.458018 4.2:-21075748 MS.gene066724:CDS
TATTCTTGATAGAAAATCAA+TGG 0.458066 4.2:-21076516 MS.gene066724:CDS
GGTGCTGGTGGTCATTTCTC+TGG 0.459504 4.2:-21076623 MS.gene066724:CDS
TCTGGGGTCTGCTTTACTCT+AGG 0.464774 4.2:-21076644 MS.gene066724:CDS
AGCAACAACACCGAAACCTT+TGG 0.466175 4.2:-21076936 MS.gene066724:CDS
CTCGGAGAATTCGAAACATA+AGG 0.468696 4.2:+21075771 None:intergenic
GTCTGCTTTACTCTAGGTGC+TGG 0.472600 4.2:-21076638 MS.gene066724:CDS
CATATGGATTCCATTGCATA+AGG 0.477148 4.2:+21075952 None:intergenic
TGATTCAATACCTTAATTCG+TGG 0.477340 4.2:-21075863 MS.gene066724:CDS
CAACTGATGTTGTTTACAAA+TGG 0.485694 4.2:-21076358 MS.gene066724:CDS
TCATATCTAAAGAAATTCTC+AGG 0.490816 4.2:+21075603 None:intergenic
GTGGTCATTTCTCTGGTGGT+GGG 0.501321 4.2:-21076616 MS.gene066724:CDS
AGAGTAAAGCAGACCCCAGA+TGG 0.503056 4.2:+21076647 None:intergenic
AACAAGTCAAGTATAACAAA+AGG 0.504620 4.2:+21076785 None:intergenic
AACAAGTTTCCTACTCTATG+AGG 0.510558 4.2:+21075888 None:intergenic
TGCTTTACTCTAGGTGCTGG+TGG 0.512366 4.2:-21076635 MS.gene066724:CDS
GCATTGAAAGGCAATCACTT+TGG 0.516798 4.2:+21076159 None:intergenic
TGGGCTATAAGAGGTGGTGG+TGG 0.518497 4.2:-21076479 MS.gene066724:CDS
TTTCCTACTCTATGAGGAAA+CGG 0.519543 4.2:+21075894 None:intergenic
GCTTCAAGAGGAGTACCAAT+TGG 0.520632 4.2:+21076101 None:intergenic
ATTCTTGATAGAAAATCAAT+GGG 0.522100 4.2:-21076515 MS.gene066724:CDS
GTGAGAATTTCCTTATGCAA+TGG 0.526102 4.2:-21075962 MS.gene066724:CDS
GTTGTTCAAATTGGTCAAGA+GGG 0.530168 4.2:-21076281 MS.gene066724:CDS
ATTGGTTCCGATAACTCCAC+AGG 0.531147 4.2:-21076420 MS.gene066724:CDS
ATATGGAAATTGATGATTGA+AGG 0.537491 4.2:-21075984 MS.gene066724:CDS
TATCAGCATCCCTATAATTG+TGG 0.556935 4.2:+21075739 None:intergenic
TGTTGTTCAAATTGGTCAAG+AGG 0.558808 4.2:-21076282 MS.gene066724:CDS
AGCATTGTTGGAAGAACCAA+AGG 0.559301 4.2:-21076081 MS.gene066724:CDS
GCTGGTGGTCATTTCTCTGG+TGG 0.564457 4.2:-21076620 MS.gene066724:CDS
CAAGCTTGCTTTCCCATCTG+GGG 0.567506 4.2:-21076660 MS.gene066724:CDS
AATTAATCCAAGGCATTGAA+AGG 0.570542 4.2:+21076147 None:intergenic
AGTGGCGGTCACGACTATGA+AGG 0.571849 4.2:-21076830 MS.gene066724:CDS
TTTACAAGAATCAACAGCAT+GGG 0.572516 4.2:-21076738 MS.gene066724:CDS
TGAGGAAACGGTGTTTCTGA+TGG 0.577426 4.2:+21075906 None:intergenic
GTTGAATCAGGTGCAACACT+TGG 0.578127 4.2:-21076716 MS.gene066724:CDS
TAATTGTGGAATGCCTCCCT+CGG 0.578960 4.2:+21075753 None:intergenic
TTTGCAGTTATAATCGCCAA+AGG 0.586317 4.2:+21076920 None:intergenic
TCTTCCAACAATGCTTCAAG+AGG 0.594711 4.2:+21076089 None:intergenic
ACACCGTTTCCTCATAGAGT+AGG 0.600052 4.2:-21075897 MS.gene066724:CDS
AAAAGAGTGGAATTAATCCA+AGG 0.600902 4.2:+21076137 None:intergenic
TTATGTTTCGAATTCTCCGA+GGG 0.609571 4.2:-21075769 MS.gene066724:CDS
ATTTACAAGAATCAACAGCA+TGG 0.616536 4.2:-21076739 MS.gene066724:CDS
TGTTTCGAATTCTCCGAGGG+AGG 0.634404 4.2:-21075766 MS.gene066724:CDS
GAGTAAAGCAGACCCCAGAT+GGG 0.637967 4.2:+21076648 None:intergenic
CAGCTTAGAATTCGAAGTGG+CGG 0.641139 4.2:-21076845 MS.gene066724:CDS
TTTGGTTCAAACCTAATTCG+GGG 0.645523 4.2:+21076177 None:intergenic
ATGCAATGGAATCCATATGG+TGG 0.646403 4.2:-21075948 MS.gene066724:CDS
ACATCGAAAACGATAACCTG+TGG 0.672224 4.2:+21076404 None:intergenic
CTTATGTTTCGAATTCTCCG+AGG 0.672859 4.2:-21075770 MS.gene066724:CDS
ATTCAGCTTAGAATTCGAAG+TGG 0.701549 4.2:-21076848 MS.gene066724:CDS
GTGAAAAGGAATGTCAGTGA+AGG 0.713631 4.2:-21076383 MS.gene066724:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTATTTTTTTGATAAAT+TGG + chr4.2:21076493-21076512 None:intergenic 10.0%
!! ATTCTTGATAGAAAATCAAT+GGG - chr4.2:21076025-21076044 MS.gene066724:CDS 20.0%
!! TATTCTTGATAGAAAATCAA+TGG - chr4.2:21076024-21076043 MS.gene066724:CDS 20.0%
!!! ACTTTTAATTTTGATGAGTA+AGG - chr4.2:21076336-21076355 MS.gene066724:CDS 20.0%
!!! TTTTTTTGATAAATTGGTTG+AGG + chr4.2:21076487-21076506 None:intergenic 20.0%
! AACAAGTCAAGTATAACAAA+AGG + chr4.2:21075758-21075777 None:intergenic 25.0%
! GCAAAAATTGTTGATGTTAA+GGG - chr4.2:21075998-21076017 MS.gene066724:CDS 25.0%
! GGAAATTTGATGAGAAAATT+TGG - chr4.2:21075950-21075969 MS.gene066724:CDS 25.0%
! TCATATCTAAAGAAATTCTC+AGG + chr4.2:21076940-21076959 None:intergenic 25.0%
! TGCAAAAATTGTTGATGTTA+AGG - chr4.2:21075997-21076016 MS.gene066724:CDS 25.0%
! TTTGACATTGCTAAAACTTA+TGG - chr4.2:21076856-21076875 MS.gene066724:CDS 25.0%
!! AATAGATCTTTATGCAGTTT+TGG + chr4.2:21076220-21076239 None:intergenic 25.0%
!! AGTTTCATTGGTCAATTTTT+AGG - chr4.2:21076301-21076320 MS.gene066724:CDS 25.0%
!! ATATGGAAATTGATGATTGA+AGG - chr4.2:21076556-21076575 MS.gene066724:CDS 25.0%
AAAAGAGTGGAATTAATCCA+AGG + chr4.2:21076406-21076425 None:intergenic 30.0%
AATCATTAGACCACGAATTA+AGG + chr4.2:21076690-21076709 None:intergenic 30.0%
AATTATAGGGATGCTGATAT+TGG - chr4.2:21076805-21076824 MS.gene066724:CDS 30.0%
ATTTACAAGAATCAACAGCA+TGG - chr4.2:21075801-21075820 MS.gene066724:CDS 30.0%
CAACCTAATGTTGTTCAAAT+TGG - chr4.2:21076250-21076269 MS.gene066724:CDS 30.0%
TCAAATCTCGTTACATTACT+TGG + chr4.2:21076841-21076860 None:intergenic 30.0%
TCTTTCATGGAAACTTCAAT+TGG - chr4.2:21076102-21076121 MS.gene066724:CDS 30.0%
TGACCAATTTGAACAACATT+AGG + chr4.2:21076256-21076275 None:intergenic 30.0%
TGATTCAATACCTTAATTCG+TGG - chr4.2:21076677-21076696 MS.gene066724:CDS 30.0%
TTTACAAGAATCAACAGCAT+GGG - chr4.2:21075802-21075821 MS.gene066724:CDS 30.0%
! AACGTCATGTGAATTTTTCA+AGG - chr4.2:21076725-21076744 MS.gene066724:CDS 30.0%
! ACTTTGGTTCAAACCTAATT+CGG + chr4.2:21076368-21076387 None:intergenic 30.0%
! CAACTGATGTTGTTTACAAA+TGG - chr4.2:21076182-21076201 MS.gene066724:CDS 30.0%
! TTATCGTTTTCGATGTGAAA+AGG - chr4.2:21076143-21076162 MS.gene066724:CDS 30.0%
!! AAGAAGCTCTAGAATCTATA+TGG - chr4.2:21076539-21076558 MS.gene066724:CDS 30.0%
!! AATTAATCCAAGGCATTGAA+AGG + chr4.2:21076396-21076415 None:intergenic 30.0%
!! ATAGATTCTAGAGCTTCTTT+AGG + chr4.2:21076538-21076557 None:intergenic 30.0%
!! TATGGAAGCAAGTTTTTCAA+AGG - chr4.2:21076874-21076893 MS.gene066724:CDS 30.0%
!!! AATGGGAGAAGATCTTTTTT+GGG - chr4.2:21076042-21076061 MS.gene066724:CDS 30.0%
!!! AATTCCACTCTTTTTTGGTA+TGG - chr4.2:21076412-21076431 MS.gene066724:CDS 30.0%
!!! GGATTAATTCCACTCTTTTT+TGG - chr4.2:21076407-21076426 MS.gene066724:CDS 30.0%
!!! GTTTTGGTGTTATTCTTTCA+TGG - chr4.2:21076089-21076108 MS.gene066724:CDS 30.0%
AACAAGTTTCCTACTCTATG+AGG + chr4.2:21076655-21076674 None:intergenic 35.0%
AATTGGTCAAGAGGGAAAAA+AGG - chr4.2:21076267-21076286 MS.gene066724:CDS 35.0%
ATTCAGCTTAGAATTCGAAG+TGG - chr4.2:21075692-21075711 MS.gene066724:CDS 35.0%
CATATGGATTCCATTGCATA+AGG + chr4.2:21076591-21076610 None:intergenic 35.0%
CTTATGCAATGGAATCCATA+TGG - chr4.2:21076589-21076608 MS.gene066724:CDS 35.0%
GAAATTCTCAGGATCAACTT+TGG + chr4.2:21076929-21076948 None:intergenic 35.0%
GTGAGAATTTCCTTATGCAA+TGG - chr4.2:21076578-21076597 MS.gene066724:CDS 35.0%
GTTATAATCGCCAAAGGTTT+CGG + chr4.2:21075617-21075636 None:intergenic 35.0%
GTTGTTCAAATTGGTCAAGA+GGG - chr4.2:21076259-21076278 MS.gene066724:CDS 35.0%
TAAATTGGTTGAGGATCCTT+TGG + chr4.2:21076478-21076497 None:intergenic 35.0%
TATCAGCATCCCTATAATTG+TGG + chr4.2:21076804-21076823 None:intergenic 35.0%
TCTTTCATCTTTCCACCATA+TGG + chr4.2:21076607-21076626 None:intergenic 35.0%
TGTTGTTCAAATTGGTCAAG+AGG - chr4.2:21076258-21076277 MS.gene066724:CDS 35.0%
TTATGTTTCGAATTCTCCGA+GGG - chr4.2:21076771-21076790 MS.gene066724:CDS 35.0%
TTTCCTACTCTATGAGGAAA+CGG + chr4.2:21076649-21076668 None:intergenic 35.0%
TTTGCAGTTATAATCGCCAA+AGG + chr4.2:21075623-21075642 None:intergenic 35.0%
! CTTTGGTTCAAACCTAATTC+GGG + chr4.2:21076367-21076386 None:intergenic 35.0%
! GTAGTGCAAGTTAGTTTCAT+TGG - chr4.2:21076289-21076308 MS.gene066724:CDS 35.0%
! TCTAGAGCTTCTTTAGGAAT+AGG + chr4.2:21076532-21076551 None:intergenic 35.0%
! TGGCACATTTAACTGTTTCT+TGG + chr4.2:21075661-21075680 None:intergenic 35.0%
! TTTGGTTCAAACCTAATTCG+GGG + chr4.2:21076366-21076385 None:intergenic 35.0%
!!! AAGCTGAATGTTGTTGCTTT+TGG + chr4.2:21075681-21075700 None:intergenic 35.0%
!!! CAATGGGAGAAGATCTTTTT+TGG - chr4.2:21076041-21076060 MS.gene066724:CDS 35.0%
!!! CTTGTTGCTTTTCTTTGCAA+TGG + chr4.2:21075864-21075883 None:intergenic 35.0%
!!! GATCTTTTTTGGGCTATAAG+AGG - chr4.2:21076052-21076071 MS.gene066724:CDS 35.0%
ACATCGAAAACGATAACCTG+TGG + chr4.2:21076139-21076158 None:intergenic 40.0%
ACGATAACCTGTGGAGTTAT+CGG + chr4.2:21076130-21076149 None:intergenic 40.0%
AGCATTGTTGGAAGAACCAA+AGG - chr4.2:21076459-21076478 MS.gene066724:CDS 40.0%
AGTAAGGAATTCCCCGAATT+AGG - chr4.2:21076352-21076371 MS.gene066724:CDS 40.0%
ATGCAATGGAATCCATATGG+TGG - chr4.2:21076592-21076611 MS.gene066724:CDS 40.0%
CTCGGAGAATTCGAAACATA+AGG + chr4.2:21076772-21076791 None:intergenic 40.0%
CTGACCATACCAAAAAAGAG+TGG + chr4.2:21076419-21076438 None:intergenic 40.0%
CTTATGTTTCGAATTCTCCG+AGG - chr4.2:21076770-21076789 MS.gene066724:CDS 40.0%
GCATTGAAAGGCAATCACTT+TGG + chr4.2:21076384-21076403 None:intergenic 40.0%
GTGAAAAGGAATGTCAGTGA+AGG - chr4.2:21076157-21076176 MS.gene066724:CDS 40.0%
TCTTCCAACAATGCTTCAAG+AGG + chr4.2:21076454-21076473 None:intergenic 40.0%
! TTTTGGTATGGTCAGCCAAT+TGG - chr4.2:21076424-21076443 MS.gene066724:CDS 40.0%
!! GGAGGCATTCCACAATTATA+GGG - chr4.2:21076792-21076811 MS.gene066724:CDS 40.0%
!! TACTCCTCTTGAAGCATTGT+TGG - chr4.2:21076447-21076466 MS.gene066724:CDS 40.0%
!!! CTTTTTTGGGCTATAAGAGG+TGG - chr4.2:21076055-21076074 MS.gene066724:CDS 40.0%
ACACCGTTTCCTCATAGAGT+AGG - chr4.2:21076643-21076662 MS.gene066724:CDS 45.0%
AGCAACAACACCGAAACCTT+TGG - chr4.2:21075604-21075623 MS.gene066724:CDS 45.0%
CAGCTTAGAATTCGAAGTGG+CGG - chr4.2:21075695-21075714 MS.gene066724:CDS 45.0%
GTGATTGCCTTTCAATGCCT+TGG - chr4.2:21076386-21076405 MS.gene066724:CDS 45.0%
GTTGAATCAGGTGCAACACT+TGG - chr4.2:21075824-21075843 MS.gene066724:CDS 45.0%
TAATTGTGGAATGCCTCCCT+CGG + chr4.2:21076790-21076809 None:intergenic 45.0%
! AACAAGCTTGCTTTCCCATC+TGG - chr4.2:21075878-21075897 MS.gene066724:CDS 45.0%
! ACAAGCTTGCTTTCCCATCT+GGG - chr4.2:21075879-21075898 MS.gene066724:CDS 45.0%
! ATTGGTTCCGATAACTCCAC+AGG - chr4.2:21076120-21076139 MS.gene066724:CDS 45.0%
! TCAACAGCATGGGTTGAATC+AGG - chr4.2:21075812-21075831 MS.gene066724:CDS 45.0%
! TGAGGAAACGGTGTTTCTGA+TGG + chr4.2:21076637-21076656 None:intergenic 45.0%
! TTTTGGGCTATAAGAGGTGG+TGG - chr4.2:21076058-21076077 MS.gene066724:CDS 45.0%
!! GCTTCAAGAGGAGTACCAAT+TGG + chr4.2:21076442-21076461 None:intergenic 45.0%
!! GGGAGGCATTCCACAATTAT+AGG - chr4.2:21076791-21076810 MS.gene066724:CDS 45.0%
AGAGTAAAGCAGACCCCAGA+TGG + chr4.2:21075896-21075915 None:intergenic 50.0%
GAGTAAAGCAGACCCCAGAT+GGG + chr4.2:21075895-21075914 None:intergenic 50.0%
GTCTGCTTTACTCTAGGTGC+TGG - chr4.2:21075902-21075921 MS.gene066724:CDS 50.0%
GTGGTCATTTCTCTGGTGGT+GGG - chr4.2:21075924-21075943 MS.gene066724:CDS 50.0%
TGTTTCGAATTCTCCGAGGG+AGG - chr4.2:21076774-21076793 MS.gene066724:CDS 50.0%
! CAAGCTTGCTTTCCCATCTG+GGG - chr4.2:21075880-21075899 MS.gene066724:CDS 50.0%
! TCTGGGGTCTGCTTTACTCT+AGG - chr4.2:21075896-21075915 MS.gene066724:CDS 50.0%
!! CATTTCTCTGGTGGTGGGTA+TGG - chr4.2:21075929-21075948 MS.gene066724:CDS 50.0%
!! TGCTTTACTCTAGGTGCTGG+TGG - chr4.2:21075905-21075924 MS.gene066724:CDS 50.0%
AGTGGCGGTCACGACTATGA+AGG - chr4.2:21075710-21075729 MS.gene066724:CDS 55.0%
GCTGGTGGTCATTTCTCTGG+TGG - chr4.2:21075920-21075939 MS.gene066724:CDS 55.0%
GGTGGTCATTTCTCTGGTGG+TGG - chr4.2:21075923-21075942 MS.gene066724:CDS 55.0%
! TGGGCTATAAGAGGTGGTGG+TGG - chr4.2:21076061-21076080 MS.gene066724:CDS 55.0%
!! GGTGCTGGTGGTCATTTCTC+TGG - chr4.2:21075917-21075936 MS.gene066724:CDS 55.0%
!!! GGTGGTGGTGGTGCTAGTTT+TGG - chr4.2:21076073-21076092 MS.gene066724:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 21075575 21076987 21075575 ID=MS.gene066724
chr4.2 mRNA 21075575 21076987 21075575 ID=MS.gene066724.t1;Parent=MS.gene066724
chr4.2 exon 21075575 21076987 21075575 ID=MS.gene066724.t1.exon1;Parent=MS.gene066724.t1
chr4.2 CDS 21075575 21076987 21075575 ID=cds.MS.gene066724.t1;Parent=MS.gene066724.t1
Gene Sequence

>MS.gene066724

ATGAAAATACAAAATAAGAGATTTAAAACAGCAACAACACCGAAACCTTTGGCGATTATAACTGCAAAAGACGATTCTCATGTCCAAGAAACAGTTAAATGTGCCAAAAGCAACAACATTCAGCTTAGAATTCGAAGTGGCGGTCACGACTATGAAGGTTTTTCATATGTATCAGATGTGCCTTTTGTTATACTTGACTTGTTTAATCTCAATTCAGTTGATGTCAATTTACAAGAATCAACAGCATGGGTTGAATCAGGTGCAACACTTGGTAAGATTTATTATACCATTGCAAAGAAAAGCAACAAGCTTGCTTTCCCATCTGGGGTCTGCTTTACTCTAGGTGCTGGTGGTCATTTCTCTGGTGGTGGGTATGGAAATTTGATGAGAAAATTTGGTCTTTCTGTTGATAATATTATCGATGCAAAAATTGTTGATGTTAAGGGTAATATTCTTGATAGAAAATCAATGGGAGAAGATCTTTTTTGGGCTATAAGAGGTGGTGGTGGTGCTAGTTTTGGTGTTATTCTTTCATGGAAACTTCAATTGGTTCCGATAACTCCACAGGTTATCGTTTTCGATGTGAAAAGGAATGTCAGTGAAGGTGCAACTGATGTTGTTTACAAATGGCAATCAATTGCACCAAAACTGCATAAAGATCTATTCATTAGAGTGCAACCTAATGTTGTTCAAATTGGTCAAGAGGGAAAAAAGGTAGTGCAAGTTAGTTTCATTGGTCAATTTTTAGGAAAAGTTGAAAGACTTTTAATTTTGATGAGTAAGGAATTCCCCGAATTAGGTTTGAACCAAAGTGATTGCCTTTCAATGCCTTGGATTAATTCCACTCTTTTTTGGTATGGTCAGCCAATTGGTACTCCTCTTGAAGCATTGTTGGAAGAACCAAAGGATCCTCAACCAATTTATCAAAAAAATAAATCAGATTATGTGAAGAAACCTATTCCTAAAGAAGCTCTAGAATCTATATGGAAATTGATGATTGAAGGTGAGAATTTCCTTATGCAATGGAATCCATATGGTGGAAAGATGAAAGAGATATTGCCATCAGAAACACCGTTTCCTCATAGAGTAGGAAACTTGTTCTTGATTCAATACCTTAATTCGTGGTCTAATGATTCTTCTGAAGTTAGTGAACGTCATGTGAATTTTTCAAGGTCGTTTTATGAATTTATGACGCCTTATGTTTCGAATTCTCCGAGGGAGGCATTCCACAATTATAGGGATGCTGATATTGGTGCTAATCATCCAAGTAATGTAACGAGATTTGACATTGCTAAAACTTATGGAAGCAAGTTTTTCAAAGGAAATTTTGAAAGACTAGTTAGTGTAAAAACCAAAGTTGATCCTGAGAATTTCTTTAGATATGAACAAAGTATTCCTACTAGATCATTGTAA
Protein sequence

>MS.gene066724.t1

MKIQNKRFKTATTPKPLAIITAKDDSHVQETVKCAKSNNIQLRIRSGGHDYEGFSYVSDVPFVILDLFNLNSVDVNLQESTAWVESGATLGKIYYTIAKKSNKLAFPSGVCFTLGAGGHFSGGGYGNLMRKFGLSVDNIIDAKIVDVKGNILDRKSMGEDLFWAIRGGGGASFGVILSWKLQLVPITPQVIVFDVKRNVSEGATDVVYKWQSIAPKLHKDLFIRVQPNVVQIGQEGKKVVQVSFIGQFLGKVERLLILMSKEFPELGLNQSDCLSMPWINSTLFWYGQPIGTPLEALLEEPKDPQPIYQKNKSDYVKKPIPKEALESIWKLMIEGENFLMQWNPYGGKMKEILPSETPFPHRVGNLFLIQYLNSWSNDSSEVSERHVNFSRSFYEFMTPYVSNSPREAFHNYRDADIGANHPSNVTRFDIAKTYGSKFFKGNFERLVSVKTKVDPENFFRYEQSIPTRSL