AlfalfaGEDB | MS.gene066986

M. sativa cultivar XinJiangDaYe / MS.gene066986

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066986.t1	XP_024637061.1	92.1	76	6	0	1	76	1	76	5.40E-27	129.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066986.t1	G7JRZ2	92.1	76	6	0	1	76	1	76	3.9e-27	129.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene066986	0.800268	1.56E-48	-1.69E-46
MS.gene052253	MS.gene066986	0.807043	6.13E-50	-1.69E-46
MS.gene053758	MS.gene066986	0.827464	1.54E-54	-1.69E-46
MS.gene053912	MS.gene066986	0.808138	3.59E-50	-1.69E-46
MS.gene056659	MS.gene066986	0.802154	6.42E-49	-1.69E-46
MS.gene057749	MS.gene066986	0.82506	5.76E-54	-1.69E-46
MS.gene057823	MS.gene066986	0.818134	2.30E-52	-1.69E-46
MS.gene05886	MS.gene066986	0.827754	1.31E-54	-1.69E-46
MS.gene058928	MS.gene066986	0.824114	9.62E-54	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066986.t1	MTR_4g111880	92.105	76	6	0	1	76	1	76	5.03e-42	134
MS.gene066986.t1	MTR_4g112530	90.789	76	7	0	1	76	1	76	1.25e-39	134

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066986.t1	AT5G23060	58.333	72	27	3	7	76	2	72	1.29e-12	61.2
MS.gene066986.t1	AT5G23060	58.333	72	27	3	7	76	2	72	1.48e-12	61.2

Find 19 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAGCAGCTTTAGCTTCATT+TGG	0.355028	8.3:+8399564	None:intergenic
AGAAGAAACAATTTGGTCCT+TGG	0.396949	8.3:+8399533	None:intergenic
GGGTGAGAGAAGAAACAATT+TGG	0.450976	8.3:+8399526	None:intergenic
GAAGAGAGCAAGTAGTGAAA+TGG	0.460147	8.3:+8399593	None:intergenic
GCAGCTTTAGCTTCATTTGG+AGG	0.465654	8.3:+8399567	None:intergenic
CAGCTTTAGCTTCATTTGGA+GGG	0.498069	8.3:+8399568	None:intergenic
GGTGATTGTGTGAGGTAAAG+TGG	0.504202	8.3:+8399665	None:intergenic
GTGGAAGGAGATGGAAGTGA+AGG	0.528700	8.3:+8399684	None:intergenic
TGTTGTTGGTAGTGAAGCCA+TGG	0.530089	8.3:+8399710	None:intergenic
AGCTTTAGCTTCATTTGGAG+GGG	0.530864	8.3:+8399569	None:intergenic
TGAGGTAAAGTGGAAGGAGA+TGG	0.548724	8.3:+8399675	None:intergenic
GGCAATGGAGATTACAGCCA+TGG	0.575193	8.3:-8399727	MS.gene066986:CDS
AGTGAAATGGTGGTTGATGT+TGG	0.580317	8.3:+8399606	None:intergenic
CTAGCAACTAACAATGGCAA+TGG	0.598628	8.3:-8399742	None:intergenic
GAGAGCAAGTAGTGAAATGG+TGG	0.617797	8.3:+8399596	None:intergenic
AAGAAGGTGGTGATTGTGTG+AGG	0.618393	8.3:+8399657	None:intergenic
AGCTGCTGTTAACATCTCCA+AGG	0.626049	8.3:-8399550	MS.gene066986:CDS
AATGATATGCGTCTATGTTG+AGG	0.636496	8.3:+8399630	None:intergenic
ATTGTGTGAGGTAAAGTGGA+AGG	0.668375	8.3:+8399669	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTATGTTGAGGTTTTAAGA+AGG	+	chr8.3:8399615-8399634	None:intergenic	30.0%
	AATGATATGCGTCTATGTTG+AGG	+	chr8.3:8399626-8399645	None:intergenic	35.0%
	AGAAGAAACAATTTGGTCCT+TGG	+	chr8.3:8399723-8399742	None:intergenic	35.0%
!!!	ATGTTGAGGTTTTAAGAAGG+TGG	+	chr8.3:8399612-8399631	None:intergenic	35.0%
	ATTGTGTGAGGTAAAGTGGA+AGG	+	chr8.3:8399587-8399606	None:intergenic	40.0%
	GAAGAGAGCAAGTAGTGAAA+TGG	+	chr8.3:8399663-8399682	None:intergenic	40.0%
	GGGTGAGAGAAGAAACAATT+TGG	+	chr8.3:8399730-8399749	None:intergenic	40.0%
!	ACAGCAGCTTTAGCTTCATT+TGG	+	chr8.3:8399692-8399711	None:intergenic	40.0%
!	AGCTTTAGCTTCATTTGGAG+GGG	+	chr8.3:8399687-8399706	None:intergenic	40.0%
!	AGTGAAATGGTGGTTGATGT+TGG	+	chr8.3:8399650-8399669	None:intergenic	40.0%
!	CAGCTTTAGCTTCATTTGGA+GGG	+	chr8.3:8399688-8399707	None:intergenic	40.0%
!!!	GGAAGTGAAGGTTTTGTTGT+TGG	+	chr8.3:8399560-8399579	None:intergenic	40.0%
	AAGAAGGTGGTGATTGTGTG+AGG	+	chr8.3:8399599-8399618	None:intergenic	45.0%
	AGCTGCTGTTAACATCTCCA+AGG	-	chr8.3:8399703-8399722	MS.gene066986:CDS	45.0%
	GAGAGCAAGTAGTGAAATGG+TGG	+	chr8.3:8399660-8399679	None:intergenic	45.0%
	GGTGATTGTGTGAGGTAAAG+TGG	+	chr8.3:8399591-8399610	None:intergenic	45.0%
	TGAGGTAAAGTGGAAGGAGA+TGG	+	chr8.3:8399581-8399600	None:intergenic	45.0%
!	GCAGCTTTAGCTTCATTTGG+AGG	+	chr8.3:8399689-8399708	None:intergenic	45.0%
!!	TGTTGTTGGTAGTGAAGCCA+TGG	+	chr8.3:8399546-8399565	None:intergenic	45.0%
	GGCAATGGAGATTACAGCCA+TGG	-	chr8.3:8399526-8399545	MS.gene066986:CDS	50.0%
	GTGGAAGGAGATGGAAGTGA+AGG	+	chr8.3:8399572-8399591	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	8399524	8399751	8399524	ID=MS.gene066986
chr8.3	mRNA	8399524	8399751	8399524	ID=MS.gene066986.t1;Parent=MS.gene066986
chr8.3	exon	8399524	8399751	8399524	ID=MS.gene066986.t1.exon1;Parent=MS.gene066986.t1
chr8.3	CDS	8399524	8399751	8399524	ID=cds.MS.gene066986.t1;Parent=MS.gene066986.t1

Gene Sequence	>MS.gene066986 ATGGCAATGGAGATTACAGCCATGGCTTCACTACCAACAACAAAACCTTCACTTCCATCTCCTTCCACTTTACCTCACACAATCACCACCTTCTTAAAACCTCAACATAGACGCATATCATTACCAACATCAACCACCATTTCACTACTTGCTCTCTTCACCCCTCCAAATGAAGCTAAAGCTGCTGTTAACATCTCCAAGGACCAAATTGTTTCTTCTCTCACCCAA
Protein sequence	>MS.gene066986.t1 MAMEITAMASLPTTKPSLPSPSTLPHTITTFLKPQHRRISLPTSTTISLLALFTPPNEAKAAVNISKDQIVSSLTQ