AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene067649

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067649.t1 MTR_4g032300 96.176 340 12 1 1 339 1 340 0.0 674
MS.gene067649.t1 MTR_4g032350 81.657 338 59 1 1 335 1 338 0.0 555
MS.gene067649.t1 MTR_4g032220 72.126 348 86 2 1 339 2 347 1.22e-170 478
MS.gene067649.t1 MTR_4g032240 66.571 350 103 4 1 339 2 348 4.45e-160 451
MS.gene067649.t1 MTR_4g032330 95.522 67 3 0 273 339 17 83 1.46e-36 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene067649.t1 AT1G33970 60.061 328 128 2 1 328 6 330 8.75e-125 362
MS.gene067649.t1 AT1G33970 60.061 328 128 2 1 328 6 330 8.75e-125 362
MS.gene067649.t1 AT1G33970 60.061 328 128 2 1 328 6 330 8.75e-125 362
MS.gene067649.t1 AT1G33970 60.061 328 128 2 1 328 6 330 8.75e-125 362
MS.gene067649.t1 AT1G33970 60.806 273 104 2 1 273 6 275 6.74e-104 306
MS.gene067649.t1 AT1G33950 50.365 274 135 1 17 290 12 284 5.35e-91 275
MS.gene067649.t1 AT1G33900 47.273 275 144 1 11 285 9 282 1.88e-88 269
MS.gene067649.t1 AT1G33950 48.540 274 134 2 17 290 12 278 5.58e-84 256
MS.gene067649.t1 AT1G33960 51.504 266 128 1 15 280 36 300 1.06e-83 257
MS.gene067649.t1 AT1G33930 47.636 275 143 1 5 279 19 292 1.71e-83 256
MS.gene067649.t1 AT1G33960 51.504 266 128 1 15 280 60 324 2.45e-83 257
MS.gene067649.t1 AT4G09940 45.806 310 160 7 3 309 31 335 2.28e-82 255
MS.gene067649.t1 AT4G09950 45.399 326 166 8 4 326 5 321 2.53e-82 253
MS.gene067649.t1 AT4G09940 45.110 317 166 7 3 316 74 385 1.75e-81 254
MS.gene067649.t1 AT1G33890 47.253 273 143 1 13 285 5 276 8.39e-81 249
MS.gene067649.t1 AT1G33890 47.253 273 143 1 13 285 5 276 8.39e-81 249
MS.gene067649.t1 AT2G26820 42.222 315 169 3 16 319 2 314 1.32e-76 239
MS.gene067649.t1 AT2G26820 42.222 315 169 3 16 319 2 314 3.41e-76 239
MS.gene067649.t1 AT1G33910 47.119 295 140 7 15 303 9 293 6.06e-76 236
MS.gene067649.t1 AT2G26820 42.581 310 167 2 21 319 5 314 8.26e-75 238
MS.gene067649.t1 AT2G26820 43.878 294 154 2 21 303 5 298 1.05e-73 235
MS.gene067649.t1 AT4G09930 42.188 320 172 7 22 339 27 335 1.10e-70 223
MS.gene067649.t1 AT1G33870 43.590 234 130 1 46 279 1 232 2.45e-65 207
MS.gene067649.t1 AT1G33830 47.343 207 109 0 21 227 8 214 2.37e-64 203
MS.gene067649.t1 AT1G33880 42.029 207 114 2 21 227 23 223 2.39e-55 181
MS.gene067649.t1 AT1G33880 42.029 207 114 2 21 227 23 223 2.39e-55 181
MS.gene067649.t1 AT4G09950 42.745 255 141 5 74 326 7 258 7.08e-54 178

Find 60 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAAATAAAATACCTTTAA+AGG 0.147645 4.1:+68909654 None:intergenic
CGTAGTGTGCAGAAATTATT+TGG 0.186995 4.1:-68909780 MS.gene067649:CDS
CACATGATTGTGGTCTTTAC+CGG 0.249622 4.1:-68909741 MS.gene067649:CDS
TTGAATAACTCGTCTCTATA+AGG 0.273379 4.1:+68909090 None:intergenic
TCTGTTGGAATTGAACTTCT+TGG 0.274494 4.1:-68909909 MS.gene067649:CDS
TGACTGTCCAGAGCCTTTAA+AGG 0.319479 4.1:-68909667 MS.gene067649:intron
GAGACACTGGACGATTATTT+AGG 0.320276 4.1:-68909693 MS.gene067649:CDS
GCAACTGGTAATAGTATTCT+TGG 0.337733 4.1:-68910270 MS.gene067649:CDS
TGATGTTAAGACATTGGTTT+TGG 0.359869 4.1:-68910319 MS.gene067649:CDS
AGTTGCACTCTTGCCATTGC+CGG 0.395880 4.1:+68910287 None:intergenic
TAAAATACCTTTAAAGGCTC+TGG 0.399861 4.1:+68909660 None:intergenic
AACTCGAGGGCTAGCTCTTC+CGG 0.409222 4.1:-68910234 MS.gene067649:CDS
ATATTCAAAAGAATATATAT+TGG 0.422703 4.1:-68908918 MS.gene067649:CDS
CGAGTTATTCAAAGAACTAA+AGG 0.423588 4.1:-68909079 MS.gene067649:intron
GACAGCATGGATTCCATCCT+TGG 0.423615 4.1:+68909857 None:intergenic
ACCAATGTCTTAACATCATT+AGG 0.430269 4.1:+68910324 None:intergenic
CTCAGTGAATCGTGTTCTTA+CGG 0.439237 4.1:+68909821 None:intergenic
GGCAATGGCAAGAGTGCAAC+TGG 0.443732 4.1:-68910285 MS.gene067649:CDS
ACATGATTGTGGTCTTTACC+GGG 0.452744 4.1:-68909740 MS.gene067649:CDS
GTAGGTTGAGTCAAAGTTGA+GGG 0.472777 4.1:-68908610 MS.gene067649:intron
TTCGCTGCAGAAAGGACAAA+TGG 0.490581 4.1:-68908981 MS.gene067649:intron
TTGGTTGGACGTACCGGCAA+TGG 0.492283 4.1:-68910300 MS.gene067649:CDS
ACCTAATGATGTTAAGACAT+TGG 0.498947 4.1:-68910325 MS.gene067649:CDS
TGCATTGACTTGGCCAAGGA+TGG 0.502829 4.1:-68909870 MS.gene067649:CDS
TTCTTCCAATTCATCTCCCC+CGG 0.516121 4.1:+68909722 None:intergenic
GCTACTAGTATATCAGAGAA+TGG 0.540658 4.1:+68908413 None:intergenic
AGATGAATTGGAAGAAAATG+AGG 0.546063 4.1:-68909715 MS.gene067649:CDS
AGAGAGGCTGATTCCATGAA+AGG 0.551453 4.1:-68908941 MS.gene067649:CDS
TGTCAGTAATTCGAGTCAGC+TGG 0.561062 4.1:+68908861 None:intergenic
TACAAGGCTTGAGCTACGAT+TGG 0.570734 4.1:-68908580 MS.gene067649:CDS
AATAGTCAAGTGCATTGACT+TGG 0.573763 4.1:-68909880 MS.gene067649:CDS
TTCACACGAGGTGGTGACAC+CGG 0.575045 4.1:+68910215 None:intergenic
GAGAATGGCACAACTAGTCT+CGG 0.577852 4.1:+68908428 None:intergenic
AGTTGAGGGAAGCAACTACA+AGG 0.583604 4.1:-68908596 MS.gene067649:CDS
TAGTCTCGGCATCTCGCTTG+CGG 0.584670 4.1:+68908442 None:intergenic
TGTAGGTTGAGTCAAAGTTG+AGG 0.603125 4.1:-68908611 MS.gene067649:intron
GGAAGAAAATGAGGAGACAC+TGG 0.603743 4.1:-68909706 MS.gene067649:CDS
GTTAATGTTATCGATAGCCC+AGG 0.610949 4.1:-68910159 MS.gene067649:intron
ACTTGTCTGCATTTCACACG+AGG 0.612967 4.1:+68910203 None:intergenic
AATTGTTGACCACATGATTG+TGG 0.613289 4.1:-68909751 MS.gene067649:CDS
CAGGCAATTCTGTCTTTGTG+TGG 0.615966 4.1:-68909225 MS.gene067649:CDS
AGAACACCACAAGGACAGCA+TGG 0.616006 4.1:+68909844 None:intergenic
ACTAAGAAGACATCTTGAGC+AGG 0.618632 4.1:-68908478 MS.gene067649:CDS
TGGAATCCATGCTGTCCTTG+TGG 0.619644 4.1:-68909850 MS.gene067649:CDS
CAAGTGCATTGACTTGGCCA+AGG 0.621661 4.1:-68909874 MS.gene067649:CDS
ATGATTGTGGTCTTTACCGG+GGG 0.624456 4.1:-68909738 MS.gene067649:CDS
AGGCAATTCTGTCTTTGTGT+GGG 0.625043 4.1:-68909224 MS.gene067649:CDS
AACATGGTAGTATCACAGAA+TGG 0.631874 4.1:-68909120 MS.gene067649:CDS
AGAGCAAGCTGCAAGACTAG+AGG 0.632155 4.1:-68908553 MS.gene067649:CDS
TGTCTTAACATCATTAGGTG+AGG 0.649897 4.1:+68910329 None:intergenic
CATGATTGTGGTCTTTACCG+GGG 0.664288 4.1:-68909739 MS.gene067649:CDS
TTCTTACGGAGAACACCACA+AGG 0.679464 4.1:+68909835 None:intergenic
TTAAAGGCTCTGGACAGTCA+CGG 0.680275 4.1:+68909670 None:intergenic
TGTCTGCATTTCACACGAGG+TGG 0.683107 4.1:+68910206 None:intergenic
CGGTAAAGACCACAATCATG+TGG 0.683165 4.1:+68909742 None:intergenic
AAGAACACGATTCACTGAGG+AGG 0.684223 4.1:-68909817 MS.gene067649:CDS
ATGGTAGTATCACAGAATGG+TGG 0.686278 4.1:-68909117 MS.gene067649:CDS
CAGACAAGTGAGATGAACGA+TGG 0.694102 4.1:-68910189 MS.gene067649:CDS
GCTGACTCGAATTACTGACA+TGG 0.703069 4.1:-68908858 MS.gene067649:intron
CGTAAGAACACGATTCACTG+AGG 0.738794 4.1:-68909820 MS.gene067649:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAATACCTTTAA+AGG + chr4.1:68909149-68909168 None:intergenic 10.0%
!! ATATTCAAAAGAATATATAT+TGG - chr4.1:68909882-68909901 MS.gene067649:CDS 10.0%
!!! ATTTTATTTGATCAATTATA+TGG - chr4.1:68910163-68910182 MS.gene067649:CDS 10.0%
!!! TTATATTATCCTTTTAATTA+TGG + chr4.1:68909302-68909321 None:intergenic 10.0%
!!! TTTTAAAATCCATAATTAAA+AGG - chr4.1:68909290-68909309 MS.gene067649:intron 10.0%
!!! AAACTTTAAAAATTTCACTT+TGG - chr4.1:68909394-68909413 MS.gene067649:intron 15.0%
!!! CTTTTCTTTTTATTGTTTAT+TGG - chr4.1:68909198-68909217 MS.gene067649:CDS 15.0%
!!! TTTATTGGATTTTAAACATT+TGG - chr4.1:68909213-68909232 MS.gene067649:CDS 15.0%
!! ATCTTAACATAAGTAGTATT+TGG - chr4.1:68908671-68908690 MS.gene067649:intron 20.0%
!! TATAAGCTTTCTTAATCATT+TGG - chr4.1:68909369-68909388 MS.gene067649:intron 20.0%
!!! AAAAACATTTTTCTGGTTAA+AGG + chr4.1:68908853-68908872 None:intergenic 20.0%
!!! AAATTTTGTACAAAAACCAA+TGG + chr4.1:68910092-68910111 None:intergenic 20.0%
!!! TTGGTTTTTGTACAAAATTT+TGG - chr4.1:68910092-68910111 MS.gene067649:intron 20.0%
! ATAAAATTCGGTTGCTAAAT+AGG + chr4.1:68908775-68908794 None:intergenic 25.0%
! ATGTTAAGATGTATTTACCT+GGG + chr4.1:68908661-68908680 None:intergenic 25.0%
! TATGTTAAGATGTATTTACC+TGG + chr4.1:68908662-68908681 None:intergenic 25.0%
!! ATCCTGTAAAAACATTTTTC+TGG + chr4.1:68908860-68908879 None:intergenic 25.0%
!! GTTTCTATGGATTTATGTTT+TGG - chr4.1:68909250-68909269 MS.gene067649:intron 25.0%
!! TTCTTTTGAATATCCTTTCA+TGG + chr4.1:68909875-68909894 None:intergenic 25.0%
!!! AACCAGAAAAATGTTTTTAC+AGG - chr4.1:68908855-68908874 MS.gene067649:intron 25.0%
!!! AGTTTTTTCTGTTGATGATT+GGG - chr4.1:68908439-68908458 MS.gene067649:CDS 25.0%
!!! GATCAATTATATGGTTTTGT+AGG - chr4.1:68910172-68910191 MS.gene067649:CDS 25.0%
!!! GGATTATTTGATTTTTCTGT+TGG - chr4.1:68908876-68908895 MS.gene067649:CDS 25.0%
!!! TTGTACAAAATTTTGGAAGT+AGG - chr4.1:68910099-68910118 MS.gene067649:intron 25.0%
AAAAAAAGTCTTCAACTCGA+GGG - chr4.1:68908553-68908572 MS.gene067649:CDS 30.0%
AATCATTATATGCACAGAAG+TGG - chr4.1:68910023-68910042 MS.gene067649:intron 30.0%
ACCAATGTCTTAACATCATT+AGG + chr4.1:68908479-68908498 None:intergenic 30.0%
ACCTAATGATGTTAAGACAT+TGG - chr4.1:68908475-68908494 MS.gene067649:CDS 30.0%
AGATGAATTGGAAGAAAATG+AGG - chr4.1:68909085-68909104 MS.gene067649:CDS 30.0%
ATCATTATATGCACAGAAGT+GGG - chr4.1:68910024-68910043 MS.gene067649:intron 30.0%
ATTGGAGCTTAAAAAGCAAA+GGG - chr4.1:68909900-68909919 MS.gene067649:CDS 30.0%
CGAGTTATTCAAAGAACTAA+AGG - chr4.1:68909721-68909740 MS.gene067649:CDS 30.0%
CTTGCATCAAAAATTCAACA+TGG + chr4.1:68909341-68909360 None:intergenic 30.0%
TAGACACCCATCATAAAATT+CGG + chr4.1:68908787-68908806 None:intergenic 30.0%
TAGTACTTTCTCCAAAACTT+GGG + chr4.1:68909450-68909469 None:intergenic 30.0%
TATTGGAGCTTAAAAAGCAA+AGG - chr4.1:68909899-68909918 MS.gene067649:CDS 30.0%
TTAGTACTTTCTCCAAAACT+TGG + chr4.1:68909451-68909470 None:intergenic 30.0%
TTGAATAACTCGTCTCTATA+AGG + chr4.1:68909713-68909732 None:intergenic 30.0%
TTGCCTGCATTATTAACAAA+AGG + chr4.1:68909562-68909581 None:intergenic 30.0%
! ACTTCTCTCTTTTGTAAACA+TGG - chr4.1:68909664-68909683 MS.gene067649:intron 30.0%
! AGTCCTTTTGTTAATAATGC+AGG - chr4.1:68909556-68909575 MS.gene067649:intron 30.0%
! GCTTTTCGATAACAAAACTA+AGG - chr4.1:68909610-68909629 MS.gene067649:intron 30.0%
! TAAAATACCTTTAAAGGCTC+TGG + chr4.1:68909143-68909162 None:intergenic 30.0%
! TAGCAACCGAATTTTATGAT+GGG - chr4.1:68908778-68908797 MS.gene067649:intron 30.0%
! TTAGCAACCGAATTTTATGA+TGG - chr4.1:68908777-68908796 MS.gene067649:intron 30.0%
! TTTTGTATGATACACTTCCT+AGG - chr4.1:68909513-68909532 MS.gene067649:intron 30.0%
!! TTTATGATGGGTGTCTAAAT+TGG - chr4.1:68908790-68908809 MS.gene067649:intron 30.0%
!!! CAGTTTTTTCTGTTGATGAT+TGG - chr4.1:68908438-68908457 MS.gene067649:CDS 30.0%
!!! TGATGTTAAGACATTGGTTT+TGG - chr4.1:68908481-68908500 MS.gene067649:CDS 30.0%
AAACCAATGGACACTTATGT+TGG + chr4.1:68910079-68910098 None:intergenic 35.0%
AACATGGTAGTATCACAGAA+TGG - chr4.1:68909680-68909699 MS.gene067649:CDS 35.0%
AACCAATGGACACTTATGTT+GGG + chr4.1:68910078-68910097 None:intergenic 35.0%
AATTGTTGACCACATGATTG+TGG - chr4.1:68909049-68909068 MS.gene067649:intron 35.0%
CAAATGGAGTTCAAAAACCT+AGG + chr4.1:68909533-68909552 None:intergenic 35.0%
CGTAGTGTGCAGAAATTATT+TGG - chr4.1:68909020-68909039 MS.gene067649:intron 35.0%
GAAAAAAAGTCTTCAACTCG+AGG - chr4.1:68908552-68908571 MS.gene067649:CDS 35.0%
GCAACTGGTAATAGTATTCT+TGG - chr4.1:68908530-68908549 MS.gene067649:CDS 35.0%
TAACAAAAGGACTCAGCAAA+TGG + chr4.1:68909549-68909568 None:intergenic 35.0%
TCATTATATGCACAGAAGTG+GGG - chr4.1:68910025-68910044 MS.gene067649:intron 35.0%
TCTGTTGGAATTGAACTTCT+TGG - chr4.1:68908891-68908910 MS.gene067649:CDS 35.0%
TGTCTTAACATCATTAGGTG+AGG + chr4.1:68908474-68908493 None:intergenic 35.0%
TTGGTTGTCAAATGAGTTTG+TGG - chr4.1:68908809-68908828 MS.gene067649:intron 35.0%
! AAACGTGTTTTCCCAAGTTT+TGG - chr4.1:68909436-68909455 MS.gene067649:intron 35.0%
!! AATAGTCAAGTGCATTGACT+TGG - chr4.1:68908920-68908939 MS.gene067649:CDS 35.0%
!!! GTTAAGACATTGGTTTTGGT+TGG - chr4.1:68908485-68908504 MS.gene067649:CDS 35.0%
ACATGATTGTGGTCTTTACC+GGG - chr4.1:68909060-68909079 MS.gene067649:intron 40.0%
ACTAAGAAGACATCTTGAGC+AGG - chr4.1:68910322-68910341 MS.gene067649:CDS 40.0%
AGGCAATTCTGTCTTTGTGT+GGG - chr4.1:68909576-68909595 MS.gene067649:intron 40.0%
ATGGTAGTATCACAGAATGG+TGG - chr4.1:68909683-68909702 MS.gene067649:CDS 40.0%
CACATGATTGTGGTCTTTAC+CGG - chr4.1:68909059-68909078 MS.gene067649:intron 40.0%
CAGCGAAACGATGAATTCAT+TGG + chr4.1:68909806-68909825 None:intergenic 40.0%
CTCAGTGAATCGTGTTCTTA+CGG + chr4.1:68908982-68909001 None:intergenic 40.0%
GAGACACTGGACGATTATTT+AGG - chr4.1:68909107-68909126 MS.gene067649:CDS 40.0%
GCTGCAAAAACAACAAAGAG+AGG - chr4.1:68909843-68909862 MS.gene067649:CDS 40.0%
GTTAATGTTATCGATAGCCC+AGG - chr4.1:68908641-68908660 MS.gene067649:intron 40.0%
! GTAGGTTGAGTCAAAGTTGA+GGG - chr4.1:68910190-68910209 MS.gene067649:CDS 40.0%
! TGTAGGTTGAGTCAAAGTTG+AGG - chr4.1:68910189-68910208 MS.gene067649:CDS 40.0%
!! GCATTGTGAAGCTGTTTCTA+TGG - chr4.1:68909237-68909256 MS.gene067649:intron 40.0%
AAGAACACGATTCACTGAGG+AGG - chr4.1:68908983-68909002 MS.gene067649:intron 45.0%
ACTTGTCTGCATTTCACACG+AGG + chr4.1:68908600-68908619 None:intergenic 45.0%
AGTTGAGGGAAGCAACTACA+AGG - chr4.1:68910204-68910223 MS.gene067649:CDS 45.0%
ATGATTGTGGTCTTTACCGG+GGG - chr4.1:68909062-68909081 MS.gene067649:intron 45.0%
CAGACAAGTGAGATGAACGA+TGG - chr4.1:68908611-68908630 MS.gene067649:intron 45.0%
CAGGCAATTCTGTCTTTGTG+TGG - chr4.1:68909575-68909594 MS.gene067649:intron 45.0%
CATGATTGTGGTCTTTACCG+GGG - chr4.1:68909061-68909080 MS.gene067649:intron 45.0%
CGGTAAAGACCACAATCATG+TGG + chr4.1:68909061-68909080 None:intergenic 45.0%
CGTAAGAACACGATTCACTG+AGG - chr4.1:68908980-68908999 MS.gene067649:intron 45.0%
GCTAAATAGGTCGCTAGCTA+AGG + chr4.1:68908762-68908781 None:intergenic 45.0%
GGAAGAAAATGAGGAGACAC+TGG - chr4.1:68909094-68909113 MS.gene067649:CDS 45.0%
TACAAGGCTTGAGCTACGAT+TGG - chr4.1:68910220-68910239 MS.gene067649:CDS 45.0%
TGACTGTCCAGAGCCTTTAA+AGG - chr4.1:68909133-68909152 MS.gene067649:CDS 45.0%
TGTCAGTAATTCGAGTCAGC+TGG + chr4.1:68909942-68909961 None:intergenic 45.0%
TTCATCGTTTCGCTGCAGAA+AGG - chr4.1:68909811-68909830 MS.gene067649:CDS 45.0%
TTCGCTGCAGAAAGGACAAA+TGG - chr4.1:68909819-68909838 MS.gene067649:CDS 45.0%
TTCTTACGGAGAACACCACA+AGG + chr4.1:68908968-68908987 None:intergenic 45.0%
TTCTTCCAATTCATCTCCCC+CGG + chr4.1:68909081-68909100 None:intergenic 45.0%
! AGAGAGGCTGATTCCATGAA+AGG - chr4.1:68909859-68909878 MS.gene067649:CDS 45.0%
! GAGAATGGCACAACTAGTCT+CGG + chr4.1:68910375-68910394 None:intergenic 45.0%
!! GCTGACTCGAATTACTGACA+TGG - chr4.1:68909942-68909961 MS.gene067649:intron 45.0%
!! TTAAAGGCTCTGGACAGTCA+CGG + chr4.1:68909133-68909152 None:intergenic 45.0%
!!! TTGGTTTTGGTTGGACGTAC+CGG - chr4.1:68908494-68908513 MS.gene067649:CDS 45.0%
AGAACACCACAAGGACAGCA+TGG + chr4.1:68908959-68908978 None:intergenic 50.0%
AGAGCAAGCTGCAAGACTAG+AGG - chr4.1:68910247-68910266 MS.gene067649:CDS 50.0%
AGTTGCACTCTTGCCATTGC+CGG + chr4.1:68908516-68908535 None:intergenic 50.0%
ATGGACACTTATGTTGGGCG+AGG + chr4.1:68910073-68910092 None:intergenic 50.0%
CGCCCAACATAAGTGTCCAT+TGG - chr4.1:68910073-68910092 MS.gene067649:intron 50.0%
CTTTACCGGGGGAGATGAAT+TGG - chr4.1:68909073-68909092 MS.gene067649:intron 50.0%
GACAGCATGGATTCCATCCT+TGG + chr4.1:68908946-68908965 None:intergenic 50.0%
TGGAATCCATGCTGTCCTTG+TGG - chr4.1:68908950-68908969 MS.gene067649:CDS 50.0%
TGGACACTTATGTTGGGCGA+GGG + chr4.1:68910072-68910091 None:intergenic 50.0%
TGTCTGCATTTCACACGAGG+TGG + chr4.1:68908597-68908616 None:intergenic 50.0%
!! CAAGTGCATTGACTTGGCCA+AGG - chr4.1:68908926-68908945 MS.gene067649:CDS 50.0%
!! TGCATTGACTTGGCCAAGGA+TGG - chr4.1:68908930-68908949 MS.gene067649:CDS 50.0%
ACACTTATGTTGGGCGAGGG+TGG + chr4.1:68910069-68910088 None:intergenic 55.0%
ACTTATGTTGGGCGAGGGTG+GGG + chr4.1:68910067-68910086 None:intergenic 55.0%
CACTTATGTTGGGCGAGGGT+GGG + chr4.1:68910068-68910087 None:intergenic 55.0%
GCACAGAAGTGGGGAGTTGT+GGG - chr4.1:68910034-68910053 MS.gene067649:intron 55.0%
TAGTCTCGGCATCTCGCTTG+CGG + chr4.1:68910361-68910380 None:intergenic 55.0%
TGCACAGAAGTGGGGAGTTG+TGG - chr4.1:68910033-68910052 MS.gene067649:intron 55.0%
TTCACACGAGGTGGTGACAC+CGG + chr4.1:68908588-68908607 None:intergenic 55.0%
! GGCAATGGCAAGAGTGCAAC+TGG - chr4.1:68908515-68908534 MS.gene067649:CDS 55.0%
! TTGGTTGGACGTACCGGCAA+TGG - chr4.1:68908500-68908519 MS.gene067649:CDS 55.0%
!! AACTCGAGGGCTAGCTCTTC+CGG - chr4.1:68908566-68908585 MS.gene067649:CDS 55.0%
CTTATGTTGGGCGAGGGTGG+GGG + chr4.1:68910066-68910085 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 68908425 68910397 68908425 ID=MS.gene067649
chr4.1 mRNA 68908425 68910397 68908425 ID=MS.gene067649.t1;Parent=MS.gene067649
chr4.1 exon 68910160 68910397 68910160 ID=MS.gene067649.t1.exon1;Parent=MS.gene067649.t1
chr4.1 CDS 68910160 68910397 68910160 ID=cds.MS.gene067649.t1;Parent=MS.gene067649.t1
chr4.1 exon 68909668 68909945 68909668 ID=MS.gene067649.t1.exon2;Parent=MS.gene067649.t1
chr4.1 CDS 68909668 68909945 68909668 ID=cds.MS.gene067649.t1;Parent=MS.gene067649.t1
chr4.1 exon 68909080 68909244 68909080 ID=MS.gene067649.t1.exon3;Parent=MS.gene067649.t1
chr4.1 CDS 68909080 68909244 68909080 ID=cds.MS.gene067649.t1;Parent=MS.gene067649.t1
chr4.1 exon 68908859 68908993 68908859 ID=MS.gene067649.t1.exon4;Parent=MS.gene067649.t1
chr4.1 CDS 68908859 68908993 68908859 ID=cds.MS.gene067649.t1;Parent=MS.gene067649.t1
chr4.1 exon 68908425 68908628 68908425 ID=MS.gene067649.t1.exon5;Parent=MS.gene067649.t1
chr4.1 CDS 68908425 68908628 68908425 ID=cds.MS.gene067649.t1;Parent=MS.gene067649.t1
Gene Sequence

>MS.gene067649

ATGGGTGGAAGTTCAGTTTTTTCTGTTGATGATTGGGAGTTTGCATCCTCACCTAATGATGTTAAGACATTGGTTTTGGTTGGACGTACCGGCAATGGCAAGAGTGCAACTGGTAATAGTATTCTTGGAAAAAAAGTCTTCAACTCGAGGGCTAGCTCTTCCGGTGTCACCACCTCGTGTGAAATGCAGACAAGTGAGATGAACGATGGACAGACTGTTAATGTTATCGATAGCCCAGGTAAATACATCTTAACATAAGTAGTATTTGGTGAAATAGCACTGCTAAAAATTTCACTATTTATCGATCAATTGAATAATCGAATCAATTCAGTCTCCTTAGCTAGCGACCTATTTAGCAACCGAATTTTATGATGGGTGTCTAAATTGGTTGTCAAATGAGTTTGTGGCAGAAACTTTGTTGTATACCTTTAACCAGAAAAATGTTTTTACAGGATTATTTGATTTTTCTGTTGGAATTGAACTTCTTGGCAAAGAAATAGTCAAGTGCATTGACTTGGCCAAGGATGGAATCCATGCTGTCCTTGTGGTGTTCTCCGTAAGAACACGATTCACTGAGGAGGAAGAAAATGCTCTGCGTAGTGTGCAGAAATTATTTGGAAGCAAAATTGTTGACCACATGATTGTGGTCTTTACCGGGGGAGATGAATTGGAAGAAAATGAGGAGACACTGGACGATTATTTAGGCCGTGACTGTCCAGAGCCTTTAAAGGTATTTTATTTTATTATTAATTATAACAATAATTTAGATATTGCTTTTCTTTTTATTGTTTATTGGATTTTAAACATTTGGTGCATTGTGAAGCTGTTTCTATGGATTTATGTTTTGGTAAAGTTTGACGATGACTTTTAAAATCCATAATTAAAAGGATAATATAAAACTTTCGCAATCATGCCATGTTGAATTTTTGATGCAAGTCATTAGTTATAAGCTTTCTTAATCATTTGGATAAACTTTAAAAATTTCACTTTGGCGATATAGTGTTTTCTCGTAAACGTGTTTTCCCAAGTTTTGGAGAAAGTACTAAACTTTATTCGATCTGTGTTTTTATTTACTCATGCTAGTATAATTTTGTATGATACACTTCCTAGGTTTTTGAACTCCATTTGCTGAGTCCTTTTGTTAATAATGCAGGCAATTCTGTCTTTGTGTGGGAATCGATGTGTGCTTTTCGATAACAAAACTAAGGATGAAAAGAAGCAAACTGAACAAGTTCAACAACTTCTCTCTTTTGTAAACATGGTAGTATCACAGAATGGTGGACAGCCTTATAGAGACGAGTTATTCAAAGAACTAAAGGTTGAGATTTTACAAAATTTCACAATTGCATTTCTTAAATATTTAAAAAACATTATGATACCAATGAATTCATCGTTTCGCTGCAGAAAGGACAAATGGAGCTGCAAAAACAACAAAGAGAGGCTGATTCCATGAAAGGATATTCAAAAGAATATATATTGGAGCTTAAAAAGCAAAGGGAGCAAGAATATAATGACCAGCTGACTCGAATTACTGACATGGTACTTTTCGTGTCTATATTTTGTCGTATTCATGATTTCGTCTTTCTTGTTTTTTTAACAATCATTATATGCACAGAAGTGGGGAGTTGTGGGTTCGAACCCCCACCCTCGCCCAACATAAGTGTCCATTGGTTTTTGTACAAAATTTTGGAAGTAGGAAACAAAGTATGAGATTGTTTTCATGTGCGCATAATCGAAGATTTTATTTGATCAATTATATGGTTTTGTAGGTTGAGTCAAAGTTGAGGGAAGCAACTACAAGGCTTGAGCTACGATTGGCAAAAGAGCAAGCTGCAAGACTAGAGGCAGAAAAGTATGCAAATGCTGCTCAAATGAAATCAAAGTATGAAATTGAGAAACTAAGAAGACATCTTGAGCAGGCACACGAAGAACTCCGCAAGCGAGATGCCGAGACTAGTTGTGCCATTCTCTGA
Protein sequence

>MS.gene067649.t1

MGGSSVFSVDDWEFASSPNDVKTLVLVGRTGNGKSATGNSILGKKVFNSRASSSGVTTSCEMQTSEMNDGQTVNVIDSPGLFDFSVGIELLGKEIVKCIDLAKDGIHAVLVVFSVRTRFTEEEENALRSVQKLFGSKIVDHMIVVFTGGDELEENEETLDDYLGRDCPEPLKAILSLCGNRCVLFDNKTKDEKKQTEQVQQLLSFVNMVVSQNGGQPYRDELFKELKKGQMELQKQQREADSMKGYSKEYILELKKQREQEYNDQLTRITDMVESKLREATTRLELRLAKEQAARLEAEKYANAAQMKSKYEIEKLRRHLEQAHEELRKRDAETSCAIL