Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068474.t1 | XP_024629663.1 | 97.3 | 221 | 6 | 0 | 1 | 221 | 142 | 362 | 1.60E-127 | 465.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068474.t1 | A0A2K3M5Z5 | 83.2 | 220 | 37 | 0 | 2 | 221 | 172 | 391 | 3.1e-109 | 404.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene068474 | 0.820197 | 7.79E-53 | -1.69E-46 |
| MS.gene054174 | MS.gene068474 | 0.815256 | 1.01E-51 | -1.69E-46 |
| MS.gene054175 | MS.gene068474 | 0.819478 | 1.14E-52 | -1.69E-46 |
| MS.gene061245 | MS.gene068474 | 0.809901 | 1.50E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068474.t1 | MTR_0259s0030 | 44.776 | 67 | 36 | 1 | 145 | 210 | 1 | 67 | 4.38e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGATATTGCTTCAATTTC+TGG | 0.007974 | 6.3:+65075362 | None:intergenic |
| CGCATGCTTGTACACATTTC+TGG | 0.140183 | 6.3:+65075494 | None:intergenic |
| GGAGGAGAAGATGATTATTA+TGG | 0.274921 | 6.3:-65075728 | MS.gene068474:CDS |
| TTTGTGCCTGCATTTCTTGT+TGG | 0.324093 | 6.3:+65075626 | None:intergenic |
| TTGTGCCTGCATTTCTTGTT+GGG | 0.345235 | 6.3:+65075627 | None:intergenic |
| ATCAATATGGAAGACTCTTC+AGG | 0.368401 | 6.3:-65075971 | None:intergenic |
| TACATATTTCTCCAGCCATA+TGG | 0.372583 | 6.3:+65075928 | None:intergenic |
| ACTAGATTACTTTGGTTTGA+AGG | 0.420033 | 6.3:-65075684 | MS.gene068474:CDS |
| ATTTCTGGAATGGGTAGAAC+AGG | 0.420480 | 6.3:+65075377 | None:intergenic |
| AGTAATCTAGTTCATAGATA+TGG | 0.422267 | 6.3:+65075696 | None:intergenic |
| CATGATATTGCCATTGATGA+AGG | 0.423617 | 6.3:+65075851 | None:intergenic |
| TATTGCTTCAATTTCTGGAA+TGG | 0.436281 | 6.3:+65075367 | None:intergenic |
| ATCTATGAACTAGATTACTT+TGG | 0.436637 | 6.3:-65075692 | MS.gene068474:CDS |
| TATGTAAATGAGAAGGCAAA+TGG | 0.445510 | 6.3:-65075911 | MS.gene068474:CDS |
| TAAGGCAATTATATCGTTGT+TGG | 0.451054 | 6.3:+65075883 | None:intergenic |
| ACATATTTCTCCAGCCATAT+GGG | 0.453680 | 6.3:+65075929 | None:intergenic |
| ATTGCTTCAATTTCTGGAAT+GGG | 0.462363 | 6.3:+65075368 | None:intergenic |
| ATCTCGTTATCCTTCATCAA+TGG | 0.467075 | 6.3:-65075861 | MS.gene068474:CDS |
| TTTACCTTCGCTCCAAGCCT+CGG | 0.468517 | 6.3:+65075793 | None:intergenic |
| TCAATTTCCACATGACCCCT+TGG | 0.476826 | 6.3:+65075443 | None:intergenic |
| AATTGCGGAGTGCACGTAAA+GGG | 0.478041 | 6.3:-65075761 | MS.gene068474:CDS |
| CTTCATCAATGGCAATATCA+TGG | 0.480258 | 6.3:-65075850 | MS.gene068474:CDS |
| GCTTGTACACATTTCTGGAT+AGG | 0.485454 | 6.3:+65075499 | None:intergenic |
| CTTGTACACATTTCTGGATA+GGG | 0.488425 | 6.3:+65075500 | None:intergenic |
| ACAAGTTTCACACCGAGGCT+TGG | 0.491152 | 6.3:-65075805 | MS.gene068474:CDS |
| TACAGAATTTCCCATATGGC+TGG | 0.495502 | 6.3:-65075939 | MS.gene068474:CDS |
| CAAAATTGTCGATATTAAGA+TGG | 0.502881 | 6.3:-65075588 | MS.gene068474:CDS |
| GCAATAATGAAAGGATCATA+AGG | 0.504721 | 6.3:+65075551 | None:intergenic |
| TACATACAGAATTTCCCATA+TGG | 0.505269 | 6.3:-65075943 | MS.gene068474:CDS |
| CACACCGAGGCTTGGAGCGA+AGG | 0.531269 | 6.3:-65075797 | MS.gene068474:CDS |
| CAATTGCGGAGTGCACGTAA+AGG | 0.546331 | 6.3:-65075762 | MS.gene068474:CDS |
| TGATGAAGGATAACGAGATA+AGG | 0.549179 | 6.3:+65075865 | None:intergenic |
| AACAAAACCAAGGGGTCATG+TGG | 0.559979 | 6.3:-65075450 | MS.gene068474:CDS |
| TGTGTACAAGCATGCGTGGA+TGG | 0.585979 | 6.3:-65075487 | MS.gene068474:CDS |
| GGAGAAATATGTAAATGAGA+AGG | 0.588500 | 6.3:-65075918 | MS.gene068474:CDS |
| TTTGACCCAACAAGAAATGC+AGG | 0.591301 | 6.3:-65075632 | MS.gene068474:CDS |
| GACGTGTCATGAGACTGATA+AGG | 0.595356 | 6.3:+65075401 | None:intergenic |
| CACGTAAAGGGTCTTACTGA+AGG | 0.598470 | 6.3:-65075749 | MS.gene068474:CDS |
| AGCTATCACAACAAAACCAA+GGG | 0.619142 | 6.3:-65075459 | MS.gene068474:CDS |
| CAGCTATCACAACAAAACCA+AGG | 0.662630 | 6.3:-65075460 | MS.gene068474:CDS |
| GTAAAGGGTCTTACTGAAGG+AGG | 0.665496 | 6.3:-65075746 | MS.gene068474:CDS |
| GAAATGTGTACAAGCATGCG+TGG | 0.673443 | 6.3:-65075491 | MS.gene068474:CDS |
| TGTGGAAATTGACAACATTG+AGG | 0.679288 | 6.3:-65075432 | MS.gene068474:CDS |
| GCTATCACAACAAAACCAAG+GGG | 0.737501 | 6.3:-65075458 | MS.gene068474:CDS |
| TGGGTACAAGTTTCACACCG+AGG | 0.754455 | 6.3:-65075810 | MS.gene068474:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCTATGAACTAGATTACTT+TGG | - | chr6.3:65075595-65075614 | MS.gene068474:CDS | 25.0% |
| ! | CAAAATTGTCGATATTAAGA+TGG | - | chr6.3:65075699-65075718 | MS.gene068474:CDS | 25.0% |
| !! | AGTAATCTAGTTCATAGATA+TGG | + | chr6.3:65075594-65075613 | None:intergenic | 25.0% |
| !! | ATATCGACAATTTTGTATTG+TGG | + | chr6.3:65075694-65075713 | None:intergenic | 25.0% |
| !! | GGAATATCTATTTTGTCAAT+GGG | - | chr6.3:65075458-65075477 | MS.gene068474:CDS | 25.0% |
| !! | TATCGACAATTTTGTATTGT+GGG | + | chr6.3:65075693-65075712 | None:intergenic | 25.0% |
| !! | TGGAATATCTATTTTGTCAA+TGG | - | chr6.3:65075457-65075476 | MS.gene068474:CDS | 25.0% |
| !!! | TTGCACTTTTTTATTGTGAA+TGG | - | chr6.3:65075632-65075651 | MS.gene068474:CDS | 25.0% |
| CAAGATATTGCTTCAATTTC+TGG | + | chr6.3:65075928-65075947 | None:intergenic | 30.0% | |
| GCAATAATGAAAGGATCATA+AGG | + | chr6.3:65075739-65075758 | None:intergenic | 30.0% | |
| GGAGAAATATGTAAATGAGA+AGG | - | chr6.3:65075369-65075388 | MS.gene068474:CDS | 30.0% | |
| GGTAAAAAAACTGTCAATTG+CGG | - | chr6.3:65075511-65075530 | MS.gene068474:CDS | 30.0% | |
| TAAGGCAATTATATCGTTGT+TGG | + | chr6.3:65075407-65075426 | None:intergenic | 30.0% | |
| TACATACAGAATTTCCCATA+TGG | - | chr6.3:65075344-65075363 | MS.gene068474:CDS | 30.0% | |
| TATGTAAATGAGAAGGCAAA+TGG | - | chr6.3:65075376-65075395 | MS.gene068474:CDS | 30.0% | |
| ! | GCTTTTTGTGCAATAATGAA+AGG | + | chr6.3:65075748-65075767 | None:intergenic | 30.0% |
| ! | TATTGCTTCAATTTCTGGAA+TGG | + | chr6.3:65075923-65075942 | None:intergenic | 30.0% |
| !! | ACTAGATTACTTTGGTTTGA+AGG | - | chr6.3:65075603-65075622 | MS.gene068474:CDS | 30.0% |
| !! | ATTGCTTCAATTTCTGGAAT+GGG | + | chr6.3:65075922-65075941 | None:intergenic | 30.0% |
| ACATATTTCTCCAGCCATAT+GGG | + | chr6.3:65075361-65075380 | None:intergenic | 35.0% | |
| AGCTATCACAACAAAACCAA+GGG | - | chr6.3:65075828-65075847 | MS.gene068474:CDS | 35.0% | |
| ATCTCGTTATCCTTCATCAA+TGG | - | chr6.3:65075426-65075445 | MS.gene068474:CDS | 35.0% | |
| CATGATATTGCCATTGATGA+AGG | + | chr6.3:65075439-65075458 | None:intergenic | 35.0% | |
| CTTCATCAATGGCAATATCA+TGG | - | chr6.3:65075437-65075456 | MS.gene068474:CDS | 35.0% | |
| GGAGGAGAAGATGATTATTA+TGG | - | chr6.3:65075559-65075578 | MS.gene068474:CDS | 35.0% | |
| TACATATTTCTCCAGCCATA+TGG | + | chr6.3:65075362-65075381 | None:intergenic | 35.0% | |
| TATTGCACAAAAAGCAAGAC+AGG | - | chr6.3:65075753-65075772 | MS.gene068474:CDS | 35.0% | |
| TGATGAAGGATAACGAGATA+AGG | + | chr6.3:65075425-65075444 | None:intergenic | 35.0% | |
| TGTGGAAATTGACAACATTG+AGG | - | chr6.3:65075855-65075874 | MS.gene068474:CDS | 35.0% | |
| ! | CTTGTACACATTTCTGGATA+GGG | + | chr6.3:65075790-65075809 | None:intergenic | 35.0% |
| CAGCTATCACAACAAAACCA+AGG | - | chr6.3:65075827-65075846 | MS.gene068474:CDS | 40.0% | |
| GCTATCACAACAAAACCAAG+GGG | - | chr6.3:65075829-65075848 | MS.gene068474:CDS | 40.0% | |
| TACAGAATTTCCCATATGGC+TGG | - | chr6.3:65075348-65075367 | MS.gene068474:CDS | 40.0% | |
| TTGTGCCTGCATTTCTTGTT+GGG | + | chr6.3:65075663-65075682 | None:intergenic | 40.0% | |
| TTTGACCCAACAAGAAATGC+AGG | - | chr6.3:65075655-65075674 | MS.gene068474:CDS | 40.0% | |
| TTTGTGCCTGCATTTCTTGT+TGG | + | chr6.3:65075664-65075683 | None:intergenic | 40.0% | |
| ! | GCTTGTACACATTTCTGGAT+AGG | + | chr6.3:65075791-65075810 | None:intergenic | 40.0% |
| !! | ATTTCTGGAATGGGTAGAAC+AGG | + | chr6.3:65075913-65075932 | None:intergenic | 40.0% |
| AACAAAACCAAGGGGTCATG+TGG | - | chr6.3:65075837-65075856 | MS.gene068474:CDS | 45.0% | |
| AATTGCGGAGTGCACGTAAA+GGG | - | chr6.3:65075526-65075545 | MS.gene068474:CDS | 45.0% | |
| CACGTAAAGGGTCTTACTGA+AGG | - | chr6.3:65075538-65075557 | MS.gene068474:CDS | 45.0% | |
| CGCATGCTTGTACACATTTC+TGG | + | chr6.3:65075796-65075815 | None:intergenic | 45.0% | |
| GAAATGTGTACAAGCATGCG+TGG | - | chr6.3:65075796-65075815 | MS.gene068474:CDS | 45.0% | |
| GACGTGTCATGAGACTGATA+AGG | + | chr6.3:65075889-65075908 | None:intergenic | 45.0% | |
| GTAAAGGGTCTTACTGAAGG+AGG | - | chr6.3:65075541-65075560 | MS.gene068474:CDS | 45.0% | |
| TCAATTTCCACATGACCCCT+TGG | + | chr6.3:65075847-65075866 | None:intergenic | 45.0% | |
| ACAAGTTTCACACCGAGGCT+TGG | - | chr6.3:65075482-65075501 | MS.gene068474:CDS | 50.0% | |
| CAATTGCGGAGTGCACGTAA+AGG | - | chr6.3:65075525-65075544 | MS.gene068474:CDS | 50.0% | |
| TGGGTACAAGTTTCACACCG+AGG | - | chr6.3:65075477-65075496 | MS.gene068474:CDS | 50.0% | |
| TGTGTACAAGCATGCGTGGA+TGG | - | chr6.3:65075800-65075819 | MS.gene068474:CDS | 50.0% | |
| TTTACCTTCGCTCCAAGCCT+CGG | + | chr6.3:65075497-65075516 | None:intergenic | 50.0% | |
| CACACCGAGGCTTGGAGCGA+AGG | - | chr6.3:65075490-65075509 | MS.gene068474:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 65075322 | 65075987 | 65075322 | ID=MS.gene068474 |
| chr6.3 | mRNA | 65075322 | 65075987 | 65075322 | ID=MS.gene068474.t1;Parent=MS.gene068474 |
| chr6.3 | exon | 65075322 | 65075987 | 65075322 | ID=MS.gene068474.t1.exon1;Parent=MS.gene068474.t1 |
| chr6.3 | CDS | 65075322 | 65075987 | 65075322 | ID=cds.MS.gene068474.t1;Parent=MS.gene068474.t1 |
| Gene Sequence |
| Protein sequence |
