Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061245.t1 | XP_024626105.1 | 85.7 | 189 | 27 | 0 | 1 | 189 | 1 | 189 | 4.40E-70 | 274.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061245.t1 | A0A396HFV7 | 62.7 | 193 | 68 | 2 | 1 | 189 | 1 | 193 | 3.9e-44 | 187.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051932 | MS.gene061245 | 0.909327 | 6.44E-82 | -1.69E-46 |
| MS.gene053491 | MS.gene061245 | 0.88471 | 1.52E-71 | -1.69E-46 |
| MS.gene054174 | MS.gene061245 | 0.813523 | 2.45E-51 | -1.69E-46 |
| MS.gene054175 | MS.gene061245 | 0.800429 | 1.45E-48 | -1.69E-46 |
| MS.gene06044 | MS.gene061245 | 0.873527 | 1.37E-67 | -1.69E-46 |
| MS.gene061245 | MS.gene068474 | 0.809901 | 1.50E-50 | -1.69E-46 |
| MS.gene061245 | MS.gene073742 | 0.821577 | 3.75E-53 | -1.69E-46 |
| MS.gene061245 | MS.gene41006 | 0.834895 | 2.30E-56 | -1.69E-46 |
| MS.gene061245 | MS.gene42436 | 0.852889 | 3.43E-61 | -1.69E-46 |
| MS.gene061245 | MS.gene45017 | 0.812478 | 4.15E-51 | -1.69E-46 |
| MS.gene061245 | MS.gene46426 | 0.836381 | 9.68E-57 | -1.69E-46 |
| MS.gene061245 | MS.gene55278 | 0.884186 | 2.38E-71 | -1.69E-46 |
| MS.gene061245 | MS.gene66513 | 0.873224 | 1.74E-67 | -1.69E-46 |
| MS.gene061245 | MS.gene66907 | 0.803709 | 3.06E-49 | -1.69E-46 |
| MS.gene061245 | MS.gene81140 | 0.835005 | 2.16E-56 | -1.69E-46 |
| MS.gene061245 | MS.gene81193 | 0.838031 | 3.66E-57 | -1.69E-46 |
| MS.gene061245 | MS.gene91022 | 0.801305 | 9.59E-49 | -1.69E-46 |
| MS.gene061245 | MS.gene93845 | 0.881778 | 1.81E-70 | -1.69E-46 |
| MS.gene061245 | MS.gene96600 | 0.852564 | 4.25E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene061245.t1 | MTR_8g007240 | 32.759 | 174 | 67 | 7 | 17 | 188 | 49 | 174 | 3.18e-15 | 70.5 |
| MS.gene061245.t1 | MTR_0903s0010 | 30.055 | 183 | 72 | 6 | 14 | 187 | 42 | 177 | 3.33e-15 | 70.5 |
| MS.gene061245.t1 | MTR_3g102970 | 31.361 | 169 | 66 | 7 | 17 | 183 | 49 | 169 | 8.28e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGAAATTGGAATTGAATT+TGG | 0.155038 | 8.3:-6015099 | MS.gene061245:CDS |
| AAAATCCAAAGAGGAAATTT+TGG | 0.165439 | 8.3:-6015467 | MS.gene061245:CDS |
| TCTGATTACTTAAAGCAATT+TGG | 0.204618 | 8.3:-6015135 | MS.gene061245:CDS |
| CATCTCCAAAATTTCCTCTT+TGG | 0.214318 | 8.3:+6015462 | None:intergenic |
| TTTGGAGATGTCGAAACTTT+TGG | 0.226089 | 8.3:-6015449 | MS.gene061245:CDS |
| ACCATTGTAATTTGTTCATA+TGG | 0.234426 | 8.3:-6015335 | MS.gene061245:CDS |
| AATTGAATTTGGAAAAGAAT+TGG | 0.290392 | 8.3:-6015088 | MS.gene061245:CDS |
| ATTGAATTTGGAAAAGAATT+GGG | 0.336217 | 8.3:-6015087 | MS.gene061245:CDS |
| TTTGGTAAATTTCCTCCAAT+TGG | 0.356380 | 8.3:+6015537 | None:intergenic |
| AAAGAGATTGGGAAGGAAAT+TGG | 0.360942 | 8.3:-6015111 | MS.gene061245:CDS |
| AAGCAATTTGGCAAAGAGAT+TGG | 0.365860 | 8.3:-6015123 | MS.gene061245:CDS |
| CCATTGTAATTTGTTCATAT+GGG | 0.394671 | 8.3:-6015334 | MS.gene061245:CDS |
| CCCAACTACAGCCTTCTTTG+AGG | 0.420158 | 8.3:+6015179 | None:intergenic |
| AGCAATTTGGCAAAGAGATT+GGG | 0.422113 | 8.3:-6015122 | MS.gene061245:CDS |
| TCAGCTGTTGGGTATCCAAT+TGG | 0.433222 | 8.3:-6015552 | MS.gene061245:CDS |
| TTAAGCATTGCTCTAACTCT+AGG | 0.452986 | 8.3:+6015294 | None:intergenic |
| GGTAGCAAAGCTTCAGCTGT+TGG | 0.475125 | 8.3:-6015564 | MS.gene061245:CDS |
| TGTTTGCACTTGTGTTGTCA+TGG | 0.475182 | 8.3:-6014975 | MS.gene061245:CDS |
| GTAGCAAAGCTTCAGCTGTT+GGG | 0.491512 | 8.3:-6015563 | MS.gene061245:CDS |
| TGAGGACACATCATTATTTG+AGG | 0.495657 | 8.3:+6015197 | None:intergenic |
| TCCTCAAAGAAGGCTGTAGT+TGG | 0.512316 | 8.3:-6015180 | MS.gene061245:CDS |
| TAATGATGTGTCCTCAAAGA+AGG | 0.517374 | 8.3:-6015190 | MS.gene061245:CDS |
| TTCCAGTACCTTTCAAAATG+TGG | 0.526356 | 8.3:-6015220 | MS.gene061245:CDS |
| GTAATCAGATGATGAAATTG+TGG | 0.528968 | 8.3:-6015506 | MS.gene061245:CDS |
| TATGTGCGTGAAAGTGTCTC+TGG | 0.539920 | 8.3:-6015057 | MS.gene061245:CDS |
| ATCTGATTACAACCACACTT+TGG | 0.542708 | 8.3:+6015519 | None:intergenic |
| GCTGTTGGGTATCCAATTGG+AGG | 0.550598 | 8.3:-6015549 | MS.gene061245:CDS |
| CCTCAAAGAAGGCTGTAGTT+GGG | 0.553172 | 8.3:-6015179 | MS.gene061245:CDS |
| TAATCAGATGATGAAATTGT+GGG | 0.555715 | 8.3:-6015505 | MS.gene061245:CDS |
| ATTTGGAAAAGAATTGGGCA+AGG | 0.572988 | 8.3:-6015082 | MS.gene061245:CDS |
| GGAGGAAATTTACCAAAGTG+TGG | 0.573900 | 8.3:-6015531 | MS.gene061245:CDS |
| ACAACACAAGTGCAAACAGT+AGG | 0.614937 | 8.3:+6014980 | None:intergenic |
| CCCATATGAACAAATTACAA+TGG | 0.636074 | 8.3:+6015334 | None:intergenic |
| ATTGCTGCAAAGAACAGTGA+AGG | 0.636453 | 8.3:-6015267 | MS.gene061245:CDS |
| ATACAGATGAAAATCCAAAG+AGG | 0.650224 | 8.3:-6015476 | MS.gene061245:CDS |
| ATTTGGCAAAGAGATTGGGA+AGG | 0.653755 | 8.3:-6015118 | MS.gene061245:CDS |
| ATTAGGGTTCCTGAAATGGG+AGG | 0.661680 | 8.3:-6015585 | None:intergenic |
| AAGAACAGTGAAGGTGATGG+TGG | 0.677445 | 8.3:-6015258 | MS.gene061245:CDS |
| GCAAAGAACAGTGAAGGTGA+TGG | 0.689878 | 8.3:-6015261 | MS.gene061245:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATTTGGAAAAGAAT+TGG | - | chr8.3:6015424-6015443 | MS.gene061245:intron | 20.0% |
| !! | ATTGAATTTGGAAAAGAATT+GGG | - | chr8.3:6015425-6015444 | MS.gene061245:intron | 20.0% |
| !!! | AAATCTGATTTGTATGTATT+TGG | - | chr8.3:6015101-6015120 | MS.gene061245:CDS | 20.0% |
| ! | AAGGAAATTGGAATTGAATT+TGG | - | chr8.3:6015413-6015432 | MS.gene061245:intron | 25.0% |
| ! | TAATCAGATGATGAAATTGT+GGG | - | chr8.3:6015007-6015026 | MS.gene061245:CDS | 25.0% |
| ! | TCTGATTACTTAAAGCAATT+TGG | - | chr8.3:6015377-6015396 | MS.gene061245:intron | 25.0% |
| !! | AAAATCCAAAGAGGAAATTT+TGG | - | chr8.3:6015045-6015064 | MS.gene061245:CDS | 25.0% |
| !!! | ACCATTGTAATTTGTTCATA+TGG | - | chr8.3:6015177-6015196 | MS.gene061245:CDS | 25.0% |
| !!! | CCATTGTAATTTGTTCATAT+GGG | - | chr8.3:6015178-6015197 | MS.gene061245:CDS | 25.0% |
| ATACAGATGAAAATCCAAAG+AGG | - | chr8.3:6015036-6015055 | MS.gene061245:CDS | 30.0% | |
| CCCATATGAACAAATTACAA+TGG | + | chr8.3:6015181-6015200 | None:intergenic | 30.0% | |
| GTAATCAGATGATGAAATTG+TGG | - | chr8.3:6015006-6015025 | MS.gene061245:CDS | 30.0% | |
| TTTGGTAAATTTCCTCCAAT+TGG | + | chr8.3:6014978-6014997 | None:intergenic | 30.0% | |
| AAAGAGATTGGGAAGGAAAT+TGG | - | chr8.3:6015401-6015420 | MS.gene061245:intron | 35.0% | |
| AAGCAATTTGGCAAAGAGAT+TGG | - | chr8.3:6015389-6015408 | MS.gene061245:intron | 35.0% | |
| AGCAATTTGGCAAAGAGATT+GGG | - | chr8.3:6015390-6015409 | MS.gene061245:intron | 35.0% | |
| ATCTGATTACAACCACACTT+TGG | + | chr8.3:6014996-6015015 | None:intergenic | 35.0% | |
| ATTTGGAAAAGAATTGGGCA+AGG | - | chr8.3:6015430-6015449 | MS.gene061245:intron | 35.0% | |
| CATCTCCAAAATTTCCTCTT+TGG | + | chr8.3:6015053-6015072 | None:intergenic | 35.0% | |
| TAATGATGTGTCCTCAAAGA+AGG | - | chr8.3:6015322-6015341 | MS.gene061245:CDS | 35.0% | |
| TGAGGACACATCATTATTTG+AGG | + | chr8.3:6015318-6015337 | None:intergenic | 35.0% | |
| TTCCAGTACCTTTCAAAATG+TGG | - | chr8.3:6015292-6015311 | MS.gene061245:CDS | 35.0% | |
| ! | TTAAGCATTGCTCTAACTCT+AGG | + | chr8.3:6015221-6015240 | None:intergenic | 35.0% |
| ! | TTGGAGATGTCGAAACTTTT+GGG | - | chr8.3:6015064-6015083 | MS.gene061245:CDS | 35.0% |
| ! | TTTGGAGATGTCGAAACTTT+TGG | - | chr8.3:6015063-6015082 | MS.gene061245:CDS | 35.0% |
| !! | ATTTGAGGCCACATTTTGAA+AGG | + | chr8.3:6015303-6015322 | None:intergenic | 35.0% |
| ACAACACAAGTGCAAACAGT+AGG | + | chr8.3:6015535-6015554 | None:intergenic | 40.0% | |
| ATTGCTGCAAAGAACAGTGA+AGG | - | chr8.3:6015245-6015264 | MS.gene061245:CDS | 40.0% | |
| ATTTGGCAAAGAGATTGGGA+AGG | - | chr8.3:6015394-6015413 | MS.gene061245:intron | 40.0% | |
| GGAGGAAATTTACCAAAGTG+TGG | - | chr8.3:6014981-6015000 | MS.gene061245:CDS | 40.0% | |
| TGTTTGCACTTGTGTTGTCA+TGG | - | chr8.3:6015537-6015556 | MS.gene061245:CDS | 40.0% | |
| !!! | GATGGTGGTTTTAGCAACAT+TGG | - | chr8.3:6015269-6015288 | MS.gene061245:CDS | 40.0% |
| CCTCAAAGAAGGCTGTAGTT+GGG | - | chr8.3:6015333-6015352 | MS.gene061245:CDS | 45.0% | |
| GCAAAGAACAGTGAAGGTGA+TGG | - | chr8.3:6015251-6015270 | MS.gene061245:CDS | 45.0% | |
| GTAGCAAAGCTTCAGCTGTT+GGG | - | chr8.3:6014949-6014968 | MS.gene061245:CDS | 45.0% | |
| TCAGCTGTTGGGTATCCAAT+TGG | - | chr8.3:6014960-6014979 | MS.gene061245:CDS | 45.0% | |
| TCCTCAAAGAAGGCTGTAGT+TGG | - | chr8.3:6015332-6015351 | MS.gene061245:CDS | 45.0% | |
| ! | AAGAACAGTGAAGGTGATGG+TGG | - | chr8.3:6015254-6015273 | MS.gene061245:CDS | 45.0% |
| ! | GAGATGTCGAAACTTTTGGG+AGG | - | chr8.3:6015067-6015086 | MS.gene061245:CDS | 45.0% |
| !! | GGCCACATTTTGAAAGGTAC+TGG | + | chr8.3:6015297-6015316 | None:intergenic | 45.0% |
| !! | TATGTGCGTGAAAGTGTCTC+TGG | - | chr8.3:6015455-6015474 | MS.gene061245:CDS | 45.0% |
| CCCAACTACAGCCTTCTTTG+AGG | + | chr8.3:6015336-6015355 | None:intergenic | 50.0% | |
| GCTGTTGGGTATCCAATTGG+AGG | - | chr8.3:6014963-6014982 | MS.gene061245:CDS | 50.0% | |
| GGTAGCAAAGCTTCAGCTGT+TGG | - | chr8.3:6014948-6014967 | MS.gene061245:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 6014942 | 6015592 | 6014942 | ID=MS.gene061245 |
| chr8.3 | mRNA | 6014942 | 6015592 | 6014942 | ID=MS.gene061245.t1;Parent=MS.gene061245 |
| chr8.3 | exon | 6015446 | 6015592 | 6015446 | ID=MS.gene061245.t1.exon1;Parent=MS.gene061245.t1 |
| chr8.3 | CDS | 6015446 | 6015592 | 6015446 | ID=cds.MS.gene061245.t1;Parent=MS.gene061245.t1 |
| chr8.3 | exon | 6014942 | 6015364 | 6014942 | ID=MS.gene061245.t1.exon2;Parent=MS.gene061245.t1 |
| chr8.3 | CDS | 6014942 | 6015364 | 6014942 | ID=cds.MS.gene061245.t1;Parent=MS.gene061245.t1 |
| Gene Sequence |
| Protein sequence |
