AlfalfaGEDB | MS.gene051932

M. sativa cultivar XinJiangDaYe / MS.gene051932

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051932.t1	XP_024634111.1	84	169	23	1	1	165	25	193	4.10E-56	227.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051932.t1	A0A396HFV7	63.3	169	58	2	1	165	25	193	9.9e-36	159.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051932	MS.gene053491	0.889155	3.12E-73	-1.69E-46
MS.gene051932	MS.gene054175	0.801287	9.67E-49	-1.69E-46
MS.gene051932	MS.gene06044	0.896567	3.26E-76	-1.69E-46
MS.gene051932	MS.gene061245	0.909327	6.44E-82	-1.69E-46
MS.gene051932	MS.gene068474	0.820197	7.79E-53	-1.69E-46
MS.gene051932	MS.gene073742	0.852259	5.19E-61	-1.69E-46
MS.gene051932	MS.gene20426	0.809039	2.30E-50	-1.69E-46
MS.gene051932	MS.gene30073	0.808445	3.09E-50	-1.69E-46
MS.gene051932	MS.gene41006	0.870645	1.25E-66	-1.69E-46
MS.gene051932	MS.gene42436	0.886603	2.96E-72	-1.69E-46
MS.gene051932	MS.gene45017	0.830787	2.41E-55	-1.69E-46
MS.gene051932	MS.gene45794	0.809683	1.68E-50	-1.69E-46
MS.gene051932	MS.gene46426	0.871189	8.26E-67	-1.69E-46
MS.gene051932	MS.gene50334	0.801907	7.22E-49	-1.69E-46
MS.gene051932	MS.gene55278	0.892561	1.42E-74	-1.69E-46
MS.gene051932	MS.gene62748	0.812052	5.15E-51	-1.69E-46
MS.gene051932	MS.gene66513	0.919984	2.27E-87	-1.69E-46
MS.gene051932	MS.gene66907	0.818518	1.88E-52	-1.69E-46
MS.gene051932	MS.gene78647	0.801587	8.39E-49	-1.69E-46
MS.gene051932	MS.gene81140	0.862412	5.16E-64	-1.69E-46
MS.gene051932	MS.gene81193	0.86303	3.33E-64	-1.69E-46
MS.gene051932	MS.gene91022	0.820998	5.10E-53	-1.69E-46
MS.gene051932	MS.gene92171	0.850898	1.26E-60	-1.69E-46
MS.gene051932	MS.gene93845	0.902378	1.03E-78	-1.69E-46
MS.gene051932	MS.gene96600	0.863275	2.80E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051932.t1	MTR_8g007240	32.121	165	62	7	2	164	58	174	1.23e-11	60.1
MS.gene051932.t1	MTR_0903s0010	28.221	163	69	5	2	163	62	177	5.46e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 32 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGAAATTGGAATTGAATT+TGG	0.155038	2.2:-2964924	MS.gene051932:CDS
AAAATCCAAAGAGGAAATTT+TGG	0.165439	2.2:-2965292	MS.gene051932:CDS
TCTGATTACTTAAAGCAATT+TGG	0.204618	2.2:-2964960	MS.gene051932:CDS
CATCTCCAAAATTTCCTCTT+TGG	0.214318	2.2:+2965287	None:intergenic
TTTGGAGATGTCGAAACTTT+TGG	0.226089	2.2:-2965274	MS.gene051932:CDS
ACCATTGTAATTTGTTCATA+TGG	0.227855	2.2:-2965160	MS.gene051932:CDS
AATTGAATTTGGAAAAGAAT+TGG	0.290392	2.2:-2964913	MS.gene051932:CDS
ATTGAATTTGGAAAAGAATT+GGG	0.336217	2.2:-2964912	MS.gene051932:CDS
AAAGAGATTGGGAAGGAAAT+TGG	0.360942	2.2:-2964936	MS.gene051932:CDS
AAGCAATTTGGCAAAGAGAT+TGG	0.365860	2.2:-2964948	MS.gene051932:CDS
CCATTGTAATTTGTTCATAT+GGG	0.394671	2.2:-2965159	MS.gene051932:CDS
CCCAACTACAGCCTTCTTTG+AGG	0.420158	2.2:+2965004	None:intergenic
AGCAATTTGGCAAAGAGATT+GGG	0.422113	2.2:-2964947	MS.gene051932:CDS
TGTTTGCACTTGTGTTGTCA+TGG	0.425538	2.2:-2964800	MS.gene051932:CDS
TTAAGCATTGCTCTAACTCT+AGG	0.453670	2.2:+2965119	None:intergenic
TGAGGACACATCATTATTTG+AGG	0.495657	2.2:+2965022	None:intergenic
GTTTGCACTTGTGTTGTCAT+GGG	0.499878	2.2:-2964799	MS.gene051932:CDS
TCCTCAAAGAAGGCTGTAGT+TGG	0.512316	2.2:-2965005	MS.gene051932:CDS
TAATGATGTGTCCTCAAAGA+AGG	0.517374	2.2:-2965015	MS.gene051932:CDS
TTCCAGTACCTTTCAAAATG+TGG	0.526356	2.2:-2965045	MS.gene051932:CDS
GTAATCAGATGATGAAATTG+TGG	0.528968	2.2:-2965331	None:intergenic
TATGTGCGTGAAAGTGTCTC+TGG	0.539920	2.2:-2964882	MS.gene051932:CDS
CCTCAAAGAAGGCTGTAGTT+GGG	0.553172	2.2:-2965004	MS.gene051932:CDS
TAATCAGATGATGAAATTGT+GGG	0.555715	2.2:-2965330	None:intergenic
ATTTGGAAAAGAATTGGGCA+AGG	0.572988	2.2:-2964907	MS.gene051932:CDS
ACAACACAAGTGCAAACAGT+AGG	0.614937	2.2:+2964805	None:intergenic
CCCATATGAACAAATTACAA+TGG	0.636074	2.2:+2965159	None:intergenic
ATTGCTGCAAAGAACAGTGA+AGG	0.636453	2.2:-2965092	MS.gene051932:CDS
ATACAGATGAAAATCCAAAG+AGG	0.650224	2.2:-2965301	MS.gene051932:CDS
ATTTGGCAAAGAGATTGGGA+AGG	0.653755	2.2:-2964943	MS.gene051932:CDS
AAGAACAGTGAAGGTGATGG+TGG	0.677445	2.2:-2965083	MS.gene051932:CDS
GCAAAGAACAGTGAAGGTGA+TGG	0.689878	2.2:-2965086	MS.gene051932:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTGAATTTGGAAAAGAAT+TGG	-	chr2.2:2965177-2965196	MS.gene051932:intron	20.0%
!!	ATTGAATTTGGAAAAGAATT+GGG	-	chr2.2:2965178-2965197	MS.gene051932:intron	20.0%
!!!	AAATCTGATTTGTATGTATT+TGG	-	chr2.2:2964854-2964873	MS.gene051932:CDS	20.0%
!	AAGGAAATTGGAATTGAATT+TGG	-	chr2.2:2965166-2965185	MS.gene051932:CDS	25.0%
!	TCTGATTACTTAAAGCAATT+TGG	-	chr2.2:2965130-2965149	MS.gene051932:CDS	25.0%
!!	AAAATCCAAAGAGGAAATTT+TGG	-	chr2.2:2964798-2964817	MS.gene051932:CDS	25.0%
!!!	ACCATTGTAATTTGTTCATA+TGG	-	chr2.2:2964930-2964949	MS.gene051932:CDS	25.0%
!!!	CCATTGTAATTTGTTCATAT+GGG	-	chr2.2:2964931-2964950	MS.gene051932:CDS	25.0%
	ATACAGATGAAAATCCAAAG+AGG	-	chr2.2:2964789-2964808	MS.gene051932:CDS	30.0%
	CCCATATGAACAAATTACAA+TGG	+	chr2.2:2964934-2964953	None:intergenic	30.0%
	AAAGAGATTGGGAAGGAAAT+TGG	-	chr2.2:2965154-2965173	MS.gene051932:CDS	35.0%
	AAGCAATTTGGCAAAGAGAT+TGG	-	chr2.2:2965142-2965161	MS.gene051932:CDS	35.0%
	AGCAATTTGGCAAAGAGATT+GGG	-	chr2.2:2965143-2965162	MS.gene051932:CDS	35.0%
	ATTTGGAAAAGAATTGGGCA+AGG	-	chr2.2:2965183-2965202	MS.gene051932:intron	35.0%
	CATCTCCAAAATTTCCTCTT+TGG	+	chr2.2:2964806-2964825	None:intergenic	35.0%
	TAATGATGTGTCCTCAAAGA+AGG	-	chr2.2:2965075-2965094	MS.gene051932:CDS	35.0%
	TGAGGACACATCATTATTTG+AGG	+	chr2.2:2965071-2965090	None:intergenic	35.0%
	TTCCAGTACCTTTCAAAATG+TGG	-	chr2.2:2965045-2965064	MS.gene051932:CDS	35.0%
!	TTAAGCATTGCTCTAACTCT+AGG	+	chr2.2:2964974-2964993	None:intergenic	35.0%
!	TTGGAGATGTCGAAACTTTT+GGG	-	chr2.2:2964817-2964836	MS.gene051932:CDS	35.0%
!	TTTGGAGATGTCGAAACTTT+TGG	-	chr2.2:2964816-2964835	MS.gene051932:CDS	35.0%
!!	ATTTGAGGCCACATTTTGAA+AGG	+	chr2.2:2965056-2965075	None:intergenic	35.0%
	ACAACACAAGTGCAAACAGT+AGG	+	chr2.2:2965288-2965307	None:intergenic	40.0%
	ATTGCTGCAAAGAACAGTGA+AGG	-	chr2.2:2964998-2965017	MS.gene051932:CDS	40.0%
	ATTTGGCAAAGAGATTGGGA+AGG	-	chr2.2:2965147-2965166	MS.gene051932:CDS	40.0%
	GTTTGCACTTGTGTTGTCAT+GGG	-	chr2.2:2965291-2965310	MS.gene051932:CDS	40.0%
	TGTTTGCACTTGTGTTGTCA+TGG	-	chr2.2:2965290-2965309	MS.gene051932:CDS	40.0%
!!!	GATGGTGGTTTTAGCAACAT+TGG	-	chr2.2:2965022-2965041	MS.gene051932:CDS	40.0%
	CCTCAAAGAAGGCTGTAGTT+GGG	-	chr2.2:2965086-2965105	MS.gene051932:CDS	45.0%
	GCAAAGAACAGTGAAGGTGA+TGG	-	chr2.2:2965004-2965023	MS.gene051932:CDS	45.0%
	TCCTCAAAGAAGGCTGTAGT+TGG	-	chr2.2:2965085-2965104	MS.gene051932:CDS	45.0%
!	AAGAACAGTGAAGGTGATGG+TGG	-	chr2.2:2965007-2965026	MS.gene051932:CDS	45.0%
!	GAGATGTCGAAACTTTTGGG+AGG	-	chr2.2:2964820-2964839	MS.gene051932:CDS	45.0%
!!	GGCCACATTTTGAAAGGTAC+TGG	+	chr2.2:2965050-2965069	None:intergenic	45.0%
!!	TATGTGCGTGAAAGTGTCTC+TGG	-	chr2.2:2965208-2965227	MS.gene051932:intron	45.0%
	CCCAACTACAGCCTTCTTTG+AGG	+	chr2.2:2965089-2965108	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	2964767	2965345	2964767	ID=MS.gene051932
chr2.2	mRNA	2964767	2965345	2964767	ID=MS.gene051932.t1;Parent=MS.gene051932
chr2.2	exon	2965271	2965345	2965271	ID=MS.gene051932.t1.exon1;Parent=MS.gene051932.t1
chr2.2	CDS	2965271	2965345	2965271	ID=cds.MS.gene051932.t1;Parent=MS.gene051932.t1
chr2.2	exon	2964767	2965189	2964767	ID=MS.gene051932.t1.exon2;Parent=MS.gene051932.t1
chr2.2	CDS	2964767	2965189	2964767	ID=cds.MS.gene051932.t1;Parent=MS.gene051932.t1

Gene Sequence

>MS.gene051932

ATGATGAAATTGTGGGTATCAAATACAGATGAAAATCCAAAGAGGAAATTTTGGAGATGTCGAAACTTTTGGGAGGTAAGTGATTTGAAATCTGATTTGTATGTATTTGGTCAAAAATATATTGTAATGTAAAAATTTGATTTGTTTTTTGTGTAGAACAATTACCATTGTAATTTGTTCATATGGGATGATGAGCTTGAAACGCCTAGAGTTAGAGCAATGCTTAAAAAAATTGCTGCAAAGAACAGTGAAGGTGATGGTGGTTTTAGCAACATTGGTTCCAGTACCTTTCAAAATGTGGCCTCAAATAATGATGTGTCCTCAAAGAAGGCTGTAGTTGGGTGTGAAAACTGTTTAGTGATATCTGATTACTTAAAGCAATTTGGCAAAGAGATTGGGAAGGAAATTGGAATTGAATTTGGAAAAGAATTGGGCAAGGATTATGTGCGTGAAAGTGTCTCTGGCAAGCATCTAAAAACTAAGCAGAAGTTGCAGAATGAAAGAAAGAAAAATTCTATCCTACTGTTTGCACTTGTGTTGTCATGGGTTTTTTTTGTTGTTGTATTGAAGTTTATGTGA

Protein sequence

>MS.gene051932.t1

MMKLWVSNTDENPKRKFWRCRNFWENNYHCNLFIWDDELETPRVRAMLKKIAAKNSEGDGGFSNIGSSTFQNVASNNDVSSKKAVVGCENCLVISDYLKQFGKEIGKEIGIEFGKELGKDYVRESVSGKHLKTKQKLQNERKKNSILLFALVLSWVFFVVVLKFM