Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069123.t1 | XP_003613028.1 | 95.3 | 169 | 8 | 0 | 1 | 169 | 1 | 169 | 6.50E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069123.t1 | U5NH37 | 44.6 | 168 | 81 | 4 | 11 | 169 | 10 | 174 | 2.4e-31 | 136.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069123.t1 | G7JWC5 | 95.3 | 169 | 8 | 0 | 1 | 169 | 1 | 169 | 4.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene069123 | 0.804541 | 2.06E-49 | -1.69E-46 |
| MS.gene049915 | MS.gene069123 | 0.808933 | 2.43E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene069123 | 0.804371 | 2.23E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene069123 | 0.822122 | 2.81E-53 | -1.69E-46 |
| MS.gene05137 | MS.gene069123 | 0.805194 | 1.50E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene069123 | 0.801202 | 1.01E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene069123 | 0.826677 | 2.38E-54 | -1.69E-46 |
| MS.gene052302 | MS.gene069123 | 0.818183 | 2.24E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene069123 | 0.83738 | 5.38E-57 | -1.69E-46 |
| MS.gene052600 | MS.gene069123 | 0.80154 | 8.58E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene069123 | 0.808463 | 3.06E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene069123 | 0.818437 | 1.96E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069123.t1 | MTR_5g031860 | 95.266 | 169 | 8 | 0 | 1 | 169 | 1 | 169 | 5.52e-116 | 337 |
| MS.gene069123.t1 | MTR_5g031830 | 89.349 | 169 | 18 | 0 | 1 | 169 | 1 | 169 | 1.03e-109 | 321 |
| MS.gene069123.t1 | MTR_5g031840 | 80.000 | 160 | 31 | 1 | 11 | 169 | 4 | 163 | 1.26e-90 | 272 |
| MS.gene069123.t1 | MTR_3g479620 | 36.571 | 175 | 84 | 5 | 11 | 170 | 5 | 167 | 3.45e-25 | 95.5 |
| MS.gene069123.t1 | MTR_5g464760 | 36.471 | 170 | 98 | 4 | 6 | 168 | 6 | 172 | 4.78e-23 | 94.7 |
| MS.gene069123.t1 | MTR_3g479490 | 38.095 | 168 | 85 | 6 | 12 | 168 | 6 | 165 | 2.72e-22 | 92.8 |
| MS.gene069123.t1 | MTR_3g479520 | 36.145 | 166 | 95 | 5 | 11 | 169 | 14 | 175 | 2.33e-21 | 90.1 |
| MS.gene069123.t1 | MTR_3g479470 | 35.119 | 168 | 92 | 6 | 11 | 168 | 12 | 172 | 2.18e-20 | 87.4 |
| MS.gene069123.t1 | MTR_3g079480 | 36.364 | 165 | 94 | 4 | 11 | 168 | 20 | 180 | 2.90e-20 | 87.0 |
| MS.gene069123.t1 | MTR_0036s0220 | 34.302 | 172 | 95 | 7 | 8 | 168 | 8 | 172 | 6.75e-20 | 85.9 |
| MS.gene069123.t1 | MTR_5g464810 | 34.911 | 169 | 98 | 5 | 8 | 168 | 8 | 172 | 2.97e-19 | 84.0 |
| MS.gene069123.t1 | MTR_5g064240 | 34.911 | 169 | 98 | 5 | 8 | 168 | 8 | 172 | 2.97e-19 | 84.0 |
| MS.gene069123.t1 | MTR_4g094220 | 33.136 | 169 | 100 | 5 | 9 | 168 | 4 | 168 | 1.00e-17 | 79.7 |
| MS.gene069123.t1 | MTR_2g008210 | 32.099 | 162 | 102 | 5 | 11 | 168 | 10 | 167 | 1.03e-17 | 79.7 |
| MS.gene069123.t1 | MTR_6g014270 | 29.480 | 173 | 106 | 4 | 9 | 168 | 4 | 173 | 2.72e-16 | 75.5 |
| MS.gene069123.t1 | MTR_6g014040 | 32.558 | 172 | 97 | 8 | 11 | 168 | 9 | 175 | 1.27e-15 | 73.6 |
| MS.gene069123.t1 | MTR_6g014220 | 34.899 | 149 | 80 | 5 | 11 | 148 | 9 | 151 | 3.28e-15 | 72.4 |
| MS.gene069123.t1 | MTR_6g013900 | 31.285 | 179 | 94 | 6 | 8 | 168 | 6 | 173 | 4.03e-15 | 72.4 |
| MS.gene069123.t1 | MTR_6g013440 | 31.579 | 171 | 99 | 7 | 11 | 168 | 10 | 175 | 4.89e-15 | 72.0 |
| MS.gene069123.t1 | MTR_6g013920 | 31.285 | 179 | 94 | 5 | 8 | 168 | 6 | 173 | 6.86e-15 | 71.6 |
| MS.gene069123.t1 | MTR_6g014195 | 29.379 | 177 | 95 | 6 | 11 | 168 | 9 | 174 | 7.29e-15 | 69.7 |
| MS.gene069123.t1 | MTR_6g014186 | 29.379 | 177 | 95 | 6 | 11 | 168 | 6 | 171 | 8.11e-15 | 71.2 |
| MS.gene069123.t1 | MTR_6g014187 | 27.684 | 177 | 98 | 4 | 11 | 168 | 9 | 174 | 1.20e-14 | 70.9 |
| MS.gene069123.t1 | MTR_6g013960 | 28.249 | 177 | 97 | 5 | 11 | 168 | 9 | 174 | 1.57e-14 | 70.5 |
| MS.gene069123.t1 | MTR_6g014020 | 29.444 | 180 | 97 | 6 | 8 | 168 | 6 | 174 | 2.01e-14 | 70.5 |
| MS.gene069123.t1 | MTR_8g059425 | 30.233 | 172 | 98 | 7 | 11 | 168 | 9 | 172 | 2.74e-14 | 70.1 |
| MS.gene069123.t1 | MTR_7g013220 | 30.857 | 175 | 103 | 5 | 9 | 170 | 2 | 171 | 1.66e-13 | 67.8 |
| MS.gene069123.t1 | MTR_3g008010 | 32.192 | 146 | 88 | 5 | 11 | 148 | 9 | 151 | 6.63e-13 | 65.9 |
| MS.gene069123.t1 | MTR_6g014200 | 31.844 | 179 | 92 | 5 | 9 | 168 | 4 | 171 | 8.58e-13 | 65.5 |
| MS.gene069123.t1 | MTR_0475s0020 | 46.269 | 67 | 34 | 1 | 11 | 77 | 9 | 73 | 9.11e-13 | 63.9 |
| MS.gene069123.t1 | MTR_0288s0020 | 47.761 | 67 | 33 | 1 | 11 | 77 | 9 | 73 | 1.03e-12 | 65.5 |
| MS.gene069123.t1 | MTR_6g014250 | 29.091 | 165 | 106 | 5 | 12 | 168 | 10 | 171 | 1.21e-12 | 65.1 |
| MS.gene069123.t1 | MTR_6g014170 | 34.694 | 147 | 81 | 6 | 11 | 147 | 9 | 150 | 2.02e-12 | 64.3 |
| MS.gene069123.t1 | MTR_6g013450 | 27.586 | 174 | 108 | 5 | 8 | 168 | 6 | 174 | 2.22e-12 | 64.3 |
| MS.gene069123.t1 | MTR_6g014290 | 42.169 | 83 | 38 | 2 | 11 | 93 | 9 | 81 | 2.29e-12 | 64.3 |
| MS.gene069123.t1 | MTR_5g058320 | 26.471 | 170 | 99 | 5 | 8 | 168 | 6 | 158 | 2.83e-12 | 63.9 |
| MS.gene069123.t1 | MTR_6g011340 | 30.233 | 172 | 106 | 4 | 8 | 168 | 6 | 174 | 3.95e-12 | 63.5 |
| MS.gene069123.t1 | MTR_1g107315 | 33.113 | 151 | 91 | 5 | 12 | 155 | 8 | 155 | 8.35e-12 | 62.8 |
| MS.gene069123.t1 | MTR_0199s0010 | 27.439 | 164 | 109 | 4 | 12 | 168 | 10 | 170 | 9.35e-12 | 62.0 |
| MS.gene069123.t1 | MTR_7g013200 | 30.178 | 169 | 101 | 4 | 9 | 170 | 2 | 160 | 1.05e-11 | 62.4 |
| MS.gene069123.t1 | MTR_8g007585 | 29.861 | 144 | 91 | 4 | 12 | 148 | 10 | 150 | 1.35e-11 | 62.0 |
| MS.gene069123.t1 | MTR_7g011470 | 29.480 | 173 | 104 | 4 | 11 | 170 | 9 | 176 | 2.71e-11 | 61.2 |
| MS.gene069123.t1 | MTR_6g014300 | 29.070 | 172 | 102 | 6 | 11 | 168 | 11 | 176 | 6.80e-11 | 60.1 |
| MS.gene069123.t1 | MTR_6g014150 | 29.944 | 177 | 94 | 5 | 11 | 168 | 6 | 171 | 7.12e-11 | 60.1 |
| MS.gene069123.t1 | MTR_6g014310 | 31.429 | 175 | 102 | 7 | 7 | 168 | 6 | 175 | 7.40e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069123.t1 | AT1G22380 | 37.126 | 167 | 90 | 5 | 11 | 168 | 12 | 172 | 2.89e-24 | 98.2 |
| MS.gene069123.t1 | AT1G22370 | 37.725 | 167 | 89 | 5 | 11 | 168 | 12 | 172 | 1.35e-22 | 93.6 |
| MS.gene069123.t1 | AT1G22360 | 35.088 | 171 | 86 | 5 | 12 | 168 | 10 | 169 | 1.55e-19 | 85.1 |
| MS.gene069123.t1 | AT1G22360 | 35.088 | 171 | 86 | 5 | 12 | 168 | 10 | 169 | 1.56e-19 | 85.1 |
| MS.gene069123.t1 | AT1G22340 | 36.364 | 154 | 73 | 6 | 11 | 150 | 12 | 154 | 4.70e-19 | 83.6 |
| MS.gene069123.t1 | AT1G22400 | 32.317 | 164 | 102 | 4 | 11 | 168 | 12 | 172 | 1.54e-18 | 82.0 |
| MS.gene069123.t1 | AT1G78270 | 32.727 | 165 | 100 | 4 | 11 | 168 | 12 | 172 | 7.00e-16 | 74.7 |
| MS.gene069123.t1 | AT3G02100 | 32.335 | 167 | 96 | 5 | 6 | 161 | 7 | 167 | 4.57e-15 | 72.0 |
| MS.gene069123.t1 | AT3G55710 | 25.581 | 172 | 109 | 5 | 6 | 169 | 2 | 162 | 1.48e-12 | 65.1 |
| MS.gene069123.t1 | AT3G55700 | 26.347 | 167 | 102 | 4 | 12 | 168 | 8 | 163 | 2.16e-12 | 64.3 |
Find 30 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTAGTGCTTGTTGCTTC+TGG | 0.129870 | 5.4:+21743585 | MS.gene069123:CDS |
| GAAAATAAGTTGCATTATTT+TGG | 0.129986 | 5.4:+21743493 | MS.gene069123:CDS |
| TTTATCTGTTCCAAAGCTTT+TGG | 0.240602 | 5.4:+21743613 | MS.gene069123:CDS |
| TCAGAGTTATATACCTCTAA+TGG | 0.246805 | 5.4:-21743651 | None:intergenic |
| ACTGATTTGGCTGCTGAGTT+TGG | 0.266869 | 5.4:+21743539 | MS.gene069123:CDS |
| TATTAGTGCTTGTTGCTTCT+GGG | 0.305741 | 5.4:+21743586 | MS.gene069123:CDS |
| CTGATTTGGCTGCTGAGTTT+GGG | 0.334780 | 5.4:+21743540 | MS.gene069123:CDS |
| GAGGAGAAACATCCTGGATT+TGG | 0.341896 | 5.4:+21743395 | MS.gene069123:CDS |
| TGGTGACCTTGCTACTGATT+TGG | 0.367535 | 5.4:+21743526 | MS.gene069123:CDS |
| CTGTGTTGAGGAAAGTTATA+TGG | 0.368749 | 5.4:-21743271 | None:intergenic |
| TCATTGCACTCCAAAAGCTT+TGG | 0.388796 | 5.4:-21743623 | None:intergenic |
| CACAGTGAGGAGAAACATCC+TGG | 0.393643 | 5.4:+21743389 | MS.gene069123:CDS |
| CAGAGTTATATACCTCTAAT+GGG | 0.395783 | 5.4:-21743650 | None:intergenic |
| ATAGTCTTGAATTGAAGCTT+AGG | 0.412111 | 5.4:-21743356 | None:intergenic |
| TGCAATGAACTTCCCATTAG+AGG | 0.423671 | 5.4:+21743638 | MS.gene069123:CDS |
| GGATTTACATGGCCTTGTGC+TGG | 0.447440 | 5.4:-21743209 | None:intergenic |
| ACATCCTGGATTTGGAGAAA+AGG | 0.498115 | 5.4:+21743403 | MS.gene069123:CDS |
| TAGTCTTGAATTGAAGCTTA+GGG | 0.511757 | 5.4:-21743355 | None:intergenic |
| TCAAACTAATGAGACGGTTG+TGG | 0.517020 | 5.4:-21743301 | None:intergenic |
| AGTTCAGCTAGCTTTAGCAT+AGG | 0.517492 | 5.4:-21743230 | None:intergenic |
| ACATGGCCTTGTGCTGGACA+TGG | 0.534043 | 5.4:-21743203 | None:intergenic |
| GCTTTAGCATAGGATTTACA+TGG | 0.537832 | 5.4:-21743220 | None:intergenic |
| GAAGATCAGCACATGTGGTA+AGG | 0.553778 | 5.4:-21743180 | None:intergenic |
| CATGGCCTTGTGCTGGACAT+GGG | 0.589394 | 5.4:-21743202 | None:intergenic |
| ATCTTCCCATGTCCAGCACA+AGG | 0.599307 | 5.4:+21743197 | MS.gene069123:CDS |
| CATGGGAAGATCAGCACATG+TGG | 0.604991 | 5.4:-21743185 | None:intergenic |
| CCTGGATTTGGAGAAAAGGT+AGG | 0.615543 | 5.4:+21743407 | MS.gene069123:CDS |
| CATCATTCAAACTAATGAGA+CGG | 0.619910 | 5.4:-21743307 | None:intergenic |
| CAGCAGCCAAATCAGTAGCA+AGG | 0.634571 | 5.4:-21743532 | None:intergenic |
| TGTGGATGTACTCTGTGTTG+AGG | 0.670603 | 5.4:-21743283 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATTATATCTCTCAAAAA+AGG | - | chr5.4:21743467-21743486 | None:intergenic | 20.0% |
| !!! | ATTATTTTGGATGGAATATT+TGG | + | chr5.4:21743506-21743525 | MS.gene069123:CDS | 20.0% |
| !!! | GAAAATAAGTTGCATTATTT+TGG | + | chr5.4:21743493-21743512 | MS.gene069123:CDS | 20.0% |
| !!! | ATAAGTTGCATTATTTTGGA+TGG | + | chr5.4:21743497-21743516 | MS.gene069123:CDS | 25.0% |
| CATCATTCAAACTAATGAGA+CGG | - | chr5.4:21743310-21743329 | None:intergenic | 30.0% | |
| ! | ATAGTCTTGAATTGAAGCTT+AGG | - | chr5.4:21743359-21743378 | None:intergenic | 30.0% |
| ! | ATTTTGTCACTGAGTTTGTA+TGG | + | chr5.4:21743437-21743456 | MS.gene069123:CDS | 30.0% |
| ! | TAGTCTTGAATTGAAGCTTA+GGG | - | chr5.4:21743358-21743377 | None:intergenic | 30.0% |
| ! | TTTATCTGTTCCAAAGCTTT+TGG | + | chr5.4:21743613-21743632 | MS.gene069123:CDS | 30.0% |
| !!! | TGTGGTAAGGATTTTTTCTT+AGG | - | chr5.4:21743170-21743189 | None:intergenic | 30.0% |
| CTGTGTTGAGGAAAGTTATA+TGG | - | chr5.4:21743274-21743293 | None:intergenic | 35.0% | |
| GCTTTAGCATAGGATTTACA+TGG | - | chr5.4:21743223-21743242 | None:intergenic | 35.0% | |
| ! | TATCTCTGATTTTCACAGTG+AGG | + | chr5.4:21743376-21743395 | MS.gene069123:CDS | 35.0% |
| ! | TATTAGTGCTTGTTGCTTCT+GGG | + | chr5.4:21743586-21743605 | MS.gene069123:CDS | 35.0% |
| ACATCCTGGATTTGGAGAAA+AGG | + | chr5.4:21743403-21743422 | MS.gene069123:CDS | 40.0% | |
| TCAAACTAATGAGACGGTTG+TGG | - | chr5.4:21743304-21743323 | None:intergenic | 40.0% | |
| TCATTGCACTCCAAAAGCTT+TGG | - | chr5.4:21743626-21743645 | None:intergenic | 40.0% | |
| TGCAATGAACTTCCCATTAG+AGG | + | chr5.4:21743638-21743657 | MS.gene069123:CDS | 40.0% | |
| ! | CTATTAGTGCTTGTTGCTTC+TGG | + | chr5.4:21743585-21743604 | MS.gene069123:CDS | 40.0% |
| !! | AGTTCAGCTAGCTTTAGCAT+AGG | - | chr5.4:21743233-21743252 | None:intergenic | 40.0% |
| CCTGGATTTGGAGAAAAGGT+AGG | + | chr5.4:21743407-21743426 | MS.gene069123:CDS | 45.0% | |
| GAAGATCAGCACATGTGGTA+AGG | - | chr5.4:21743183-21743202 | None:intergenic | 45.0% | |
| GAGGAGAAACATCCTGGATT+TGG | + | chr5.4:21743395-21743414 | MS.gene069123:CDS | 45.0% | |
| TGTGGATGTACTCTGTGTTG+AGG | - | chr5.4:21743286-21743305 | None:intergenic | 45.0% | |
| ! | CCTACCTTTTCTCCAAATCC+AGG | - | chr5.4:21743410-21743429 | None:intergenic | 45.0% |
| ! | TGGTGACCTTGCTACTGATT+TGG | + | chr5.4:21743526-21743545 | MS.gene069123:CDS | 45.0% |
| !! | ACTGATTTGGCTGCTGAGTT+TGG | + | chr5.4:21743539-21743558 | MS.gene069123:CDS | 45.0% |
| !! | CTGATTTGGCTGCTGAGTTT+GGG | + | chr5.4:21743540-21743559 | MS.gene069123:CDS | 45.0% |
| ATCTTCCCATGTCCAGCACA+AGG | + | chr5.4:21743197-21743216 | MS.gene069123:CDS | 50.0% | |
| CACAGTGAGGAGAAACATCC+TGG | + | chr5.4:21743389-21743408 | MS.gene069123:CDS | 50.0% | |
| CAGCAGCCAAATCAGTAGCA+AGG | - | chr5.4:21743535-21743554 | None:intergenic | 50.0% | |
| CATGGGAAGATCAGCACATG+TGG | - | chr5.4:21743188-21743207 | None:intergenic | 50.0% | |
| !! | GGATTTACATGGCCTTGTGC+TGG | - | chr5.4:21743212-21743231 | None:intergenic | 50.0% |
| !! | ACATGGCCTTGTGCTGGACA+TGG | - | chr5.4:21743206-21743225 | None:intergenic | 55.0% |
| !! | CATGGCCTTGTGCTGGACAT+GGG | - | chr5.4:21743205-21743224 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 21743155 | 21743667 | 21743155 | ID=MS.gene069123 |
| chr5.4 | mRNA | 21743155 | 21743667 | 21743155 | ID=MS.gene069123.t1;Parent=MS.gene069123 |
| chr5.4 | exon | 21743155 | 21743667 | 21743155 | ID=MS.gene069123.t1.exon1;Parent=MS.gene069123.t1 |
| chr5.4 | CDS | 21743155 | 21743667 | 21743155 | ID=cds.MS.gene069123.t1;Parent=MS.gene069123.t1 |
| Gene Sequence |
| Protein sequence |
