AlfalfaGEDB | MS.gene069413

M. sativa cultivar XinJiangDaYe / MS.gene069413

KEGG: -
GO: GO:0000145 GO:0005575 GO:0006810 GO:0006887 GO:0008150 GO:0009987 GO:0016192 GO:0032940 GO:0032991 GO:0046903 GO:0051179 GO:0051234 GO:0099023 GO:0140352
PFAM: PF03081.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069413.t1	XP_024626193.1	100	242	0	0	1	242	436	677	1.00E-132	482.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069413.t1	Q9FNR3	48.3	230	101	3	1	230	414	625	3.1e-53	209.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069413.t1	A0A072TXQ3	100.0	242	0	0	1	242	436	677	7.5e-133	482.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050654	MS.gene069413	0.803099	4.10E-49	-1.69E-46
MS.gene052118	MS.gene069413	0.812578	3.95E-51	-1.69E-46
MS.gene054479	MS.gene069413	0.810221	1.28E-50	-1.69E-46
MS.gene054641	MS.gene069413	0.812002	5.28E-51	-1.69E-46
MS.gene055327	MS.gene069413	0.818584	1.82E-52	-1.69E-46
MS.gene055864	MS.gene069413	0.805592	1.24E-49	-1.69E-46
MS.gene058081	MS.gene069413	0.81252	4.07E-51	-1.69E-46
MS.gene058429	MS.gene069413	0.805843	1.10E-49	-1.69E-46
MS.gene060435	MS.gene069413	0.824192	9.22E-54	-1.69E-46
MS.gene06052	MS.gene069413	0.806375	8.48E-50	-1.69E-46
MS.gene060932	MS.gene069413	0.810775	9.75E-51	-1.69E-46
MS.gene06121	MS.gene069413	0.811815	5.80E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069413.t1	MTR_7g013770	100.000	242	0	0	1	242	436	677	1.65e-177	503
MS.gene069413.t1	MTR_4g103540	47.154	246	116	5	1	242	455	690	2.84e-64	212
MS.gene069413.t1	MTR_3g025900	42.798	243	124	4	1	242	391	619	8.44e-59	196
MS.gene069413.t1	MTR_8g021330	42.259	239	125	5	1	231	372	605	2.13e-51	176
MS.gene069413.t1	MTR_7g026630	35.865	237	138	5	1	231	422	650	1.47e-44	158
MS.gene069413.t1	MTR_5g073450	37.975	237	129	5	1	232	411	634	2.85e-44	157
MS.gene069413.t1	MTR_1g117090	33.846	260	142	6	1	232	412	669	8.40e-43	154
MS.gene069413.t1	MTR_1g090620	33.200	250	125	4	1	231	415	641	1.03e-41	150
MS.gene069413.t1	MTR_3g031130	36.709	237	125	3	1	232	404	620	9.76e-40	145
MS.gene069413.t1	MTR_1g057560	33.054	239	128	4	1	230	409	624	1.07e-37	139
MS.gene069413.t1	MTR_6g033310	33.473	239	148	5	1	232	492	726	1.62e-37	139
MS.gene069413.t1	MTR_2g096230	32.328	232	137	6	1	231	369	581	1.28e-33	127
MS.gene069413.t1	MTR_5g073460	35.565	239	137	6	1	234	469	695	2.17e-33	127
MS.gene069413.t1	MTR_1g067480	30.901	233	149	4	1	229	376	600	6.79e-26	105
MS.gene069413.t1	MTR_5g074130	33.898	177	113	2	63	235	503	679	7.30e-26	105
MS.gene069413.t1	MTR_1g067460	30.864	243	149	7	1	235	374	605	6.68e-25	103
MS.gene069413.t1	MTR_5g073780	33.143	175	113	2	63	234	53	226	1.22e-22	92.8
MS.gene069413.t1	MTR_5g073920	33.333	174	108	2	63	233	493	661	1.49e-22	96.3
MS.gene069413.t1	MTR_5g073620	33.714	175	112	2	63	234	898	1071	1.84e-22	96.3
MS.gene069413.t1	MTR_5g073490	32.335	167	92	3	69	230	3	153	3.47e-20	84.3
MS.gene069413.t1	MTR_4g008260	30.412	194	119	4	56	241	466	651	1.42e-19	87.8
MS.gene069413.t1	MTR_4g062330	27.193	228	153	5	1	225	381	598	2.62e-19	87.0
MS.gene069413.t1	MTR_0229s0040	28.141	199	127	4	53	237	24	220	6.25e-18	80.5
MS.gene069413.t1	MTR_3g464010	30.978	184	116	4	52	229	488	666	3.85e-17	80.5
MS.gene069413.t1	MTR_1g009620	28.141	199	127	4	53	237	469	665	5.74e-17	80.1
MS.gene069413.t1	MTR_5g093530	29.825	171	93	3	63	229	423	570	1.53e-16	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene069413.t1	AT3G29400	50.794	252	114	5	1	242	407	658	4.19e-69	224
MS.gene069413.t1	AT5G61010	48.485	231	101	4	1	231	414	626	3.50e-67	219
MS.gene069413.t1	AT5G61010	48.485	231	101	4	1	231	414	626	3.50e-67	219
MS.gene069413.t1	AT5G50380	44.922	256	112	4	1	242	442	682	1.67e-66	218
MS.gene069413.t1	AT3G14090	41.841	239	122	6	1	231	384	613	5.92e-52	178
MS.gene069413.t1	AT1G72470	40.833	240	125	6	1	231	390	621	8.03e-52	178
MS.gene069413.t1	AT1G54090	40.586	239	129	5	1	231	378	611	3.68e-49	171
MS.gene069413.t1	AT5G13150	36.100	241	137	6	1	231	411	644	1.83e-45	160
MS.gene069413.t1	AT5G03540	35.246	244	123	4	1	231	294	515	5.89e-43	152
MS.gene069413.t1	AT5G03540	35.246	244	123	4	1	231	409	630	1.61e-42	152
MS.gene069413.t1	AT5G03540	35.246	244	123	4	1	231	435	656	2.96e-42	152
MS.gene069413.t1	AT1G07725	35.043	234	130	6	1	231	377	591	2.23e-38	141
MS.gene069413.t1	AT5G52340	33.613	238	128	5	1	231	409	623	2.64e-38	141
MS.gene069413.t1	AT5G58430	35.021	237	126	5	1	232	406	619	2.50e-36	135
MS.gene069413.t1	AT5G13990	31.513	238	141	4	1	232	461	682	3.89e-36	135
MS.gene069413.t1	AT2G28640	31.064	235	149	4	1	234	359	581	1.07e-34	130
MS.gene069413.t1	AT5G52350	37.143	175	103	2	64	231	403	577	4.85e-33	126
MS.gene069413.t1	AT2G39380	29.289	239	150	4	1	232	385	611	1.24e-29	116
MS.gene069413.t1	AT1G07000	30.672	238	129	4	1	232	390	597	1.38e-29	116
MS.gene069413.t1	AT3G55150	30.544	239	146	4	1	232	386	611	2.46e-29	115
MS.gene069413.t1	AT5G59730	30.544	239	152	7	1	233	370	600	1.03e-26	108
MS.gene069413.t1	AT5G59730	30.833	240	150	8	1	233	370	600	1.12e-26	108
MS.gene069413.t1	AT3G09520	29.461	241	144	5	1	229	378	604	8.92e-24	99.8
MS.gene069413.t1	AT2G28650	28.384	229	138	6	1	229	352	554	1.62e-23	99.0
MS.gene069413.t1	AT4G31540	28.409	176	120	2	56	225	475	650	6.93e-20	88.6
MS.gene069413.t1	AT3G09530	26.582	237	162	4	1	231	372	602	2.78e-19	87.0

Find 0 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TGATTTAGAAAATTATCTTT+TGG	-	51466:4001-4020	MS.gene069413:CDS	15.0%
!	TTAAAGTGATTCAAGCTTAT+AGG	-	51466:3919-3938	MS.gene069413:CDS	25.0%
!!	TATCTTTTGGATTTCTTTGA+AGG	-	51466:4014-4033	MS.gene069413:CDS	25.0%
	AATGAACAACCTACATTACA+TGG	-	51466:3599-3618	MS.gene069413:CDS	30.0%
	AGATTTGCGGATTTCTATAT+CGG	-	51466:3896-3915	MS.gene069413:CDS	30.0%
	AGTTGTTGCAAAATCCTATT+AGG	-	51466:4045-4064	MS.gene069413:CDS	30.0%
	ATCCTTAAGAAAGTTGAGAA+TGG	+	51466:3739-3758	MS.gene069413:intergenic	30.0%
	TCTGAACTTAGGATCATATT+CGG	-	51466:3639-3658	MS.gene069413:CDS	30.0%
	TGTCTCAAAAACTCTTCTAA+AGG	-	51466:3788-3807	MS.gene069413:CDS	30.0%
	TTCCATTCTCAACTTTCTTA+AGG	-	51466:3734-3753	MS.gene069413:CDS	30.0%
	ACATGGCTGAAAAGGTTAAA+GGG	-	51466:3616-3635	MS.gene069413:CDS	35.0%
	CAAGCTTATAGGACATTTGT+TGG	-	51466:3930-3949	MS.gene069413:CDS	35.0%
	CTCAACTTTCTTAAGGATGA+AGG	-	51466:3741-3760	MS.gene069413:CDS	35.0%
	TACATGGCTGAAAAGGTTAA+AGG	-	51466:3615-3634	MS.gene069413:CDS	35.0%
	TCTGAAAGATCAAGAAGAAG+AGG	-	51466:3395-3414	MS.gene069413:CDS	35.0%
	TTGGCTTCTGTTATCATCTT+CGG	+	51466:3457-3476	MS.gene069413:intergenic	35.0%
	TTTACAGGATTCAAACAGCT+TGG	-	51466:3847-3866	MS.gene069413:CDS	35.0%
!	CTTATAGGACATTTGTTGGA+AGG	-	51466:3934-3953	MS.gene069413:CDS	35.0%
!	GAAACTTCCAGTTGTGTTTT+CGG	+	51466:3677-3696	MS.gene069413:intergenic	35.0%
!	TTTGAGACAGAATTTGAACC+AGG	+	51466:3777-3796	MS.gene069413:intergenic	35.0%
	AAGTTACGAAGCTTCTACCT+CGG	-	51466:3813-3832	MS.gene069413:CDS	40.0%
	AATGGATCCGAAAACACAAC+TGG	-	51466:3667-3686	MS.gene069413:CDS	40.0%
	AGGTTAAAGGGTCTGAACTT+AGG	-	51466:3628-3647	MS.gene069413:CDS	40.0%
	ATTCAGTGCTTCGCTGTAAT+CGG	+	51466:3370-3389	MS.gene069413:intergenic	40.0%
	CCAAGACTGAAGCAAATGAT+AGG	+	51466:3518-3537	MS.gene069413:intergenic	40.0%
	CCTACATTACATGGCTGAAA+AGG	-	51466:3608-3627	MS.gene069413:CDS	40.0%
	CCTATCATTTGCTTCAGTCT+TGG	-	51466:3515-3534	MS.gene069413:CDS	40.0%
	TGAAATACGAGAGAGCTAGT+TGG	-	51466:3709-3728	MS.gene069413:CDS	40.0%
!	CCTTTTCAGCCATGTAATGT+AGG	+	51466:3611-3630	MS.gene069413:intergenic	40.0%
	AGTCTTGGAGAACAATCTCG+AGG	-	51466:3530-3549	MS.gene069413:CDS	45.0%
	CTGTTATCATCTTCGGTACC+CGG	+	51466:3450-3469	MS.gene069413:intergenic	45.0%
	GAAATCTACCAGGAGATGCT+TGG	+	51466:3476-3495	MS.gene069413:intergenic	45.0%
	GGATCATATTCGGAGACGAA+TGG	-	51466:3649-3668	MS.gene069413:CDS	45.0%
	TCCTGGTAGATTTCCCTCAA+TGG	-	51466:3482-3501	MS.gene069413:CDS	45.0%
	TCGGCTTCGAGGATATTTAC+AGG	-	51466:3832-3851	MS.gene069413:CDS	45.0%
	TGTAAATATCCTCGAAGCCG+AGG	+	51466:3833-3852	MS.gene069413:intergenic	45.0%
	TTCAGCTTCGCGAAGATTTG+CGG	-	51466:3883-3902	MS.gene069413:CDS	45.0%
	AACAGAAGCCAAGCATCTCC+TGG	-	51466:3465-3484	MS.gene069413:CDS	50.0%
	CGTGTCAGGTGACGATGAAA+TGG	+	51466:3424-3443	MS.gene069413:intergenic	50.0%
	GATGAAGGAATTCACGCTCC+TGG	-	51466:3756-3775	MS.gene069413:CDS	50.0%
	GCCATTGAGGGAAATCTACC+AGG	+	51466:3486-3505	MS.gene069413:intergenic	50.0%
	GTAGATTTCCCTCAATGGCG+CGG	-	51466:3487-3506	MS.gene069413:CDS	50.0%
	TCTTCGCGAAGCTGAACATC+TGG	+	51466:3879-3898	MS.gene069413:intergenic	50.0%
	AAGCTTCTACCTCGGCTTCG+AGG	-	51466:3821-3840	MS.gene069413:CDS	55.0%
	AGGAATTGCCGCGCCATTGA+GGG	+	51466:3498-3517	MS.gene069413:intergenic	55.0%
	ATCGTCACCTGACACGAGTC+CGG	-	51466:3428-3447	MS.gene069413:CDS	55.0%
	TAGGAATTGCCGCGCCATTG+AGG	+	51466:3499-3518	MS.gene069413:intergenic	55.0%
	TCGTCACCTGACACGAGTCC+GGG	-	51466:3429-3448	MS.gene069413:CDS	60.0%
	TCGGTACCCGGACTCGTGTC+AGG	+	51466:3438-3457	MS.gene069413:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
51466	gene	3345	4073	3345	ID=MS.gene069413
51466	mRNA	3345	4073	3345	ID=MS.gene069413.t1;Parent=MS.gene069413
51466	exon	3345	4073	3345	ID=MS.gene069413.t1.exon1;Parent=MS.gene069413.t1
51466	CDS	3345	4073	3345	ID=cds.MS.gene069413.t1;Parent=MS.gene069413.t1

Gene Sequence

>MS.gene069413

ATGAATTATCTTCGGACTCTTACCGATTACAGCGAAGCACTGAATCATCTTCTGAAAGATCAAGAAGAAGAGGATTCCATTTCATCGTCACCTGACACGAGTCCGGGTACCGAAGATGATAACAGAAGCCAAGCATCTCCTGGTAGATTTCCCTCAATGGCGCGGCAATTCCTATCATTTGCTTCAGTCTTGGAGAACAATCTCGAGGAGAAATCGAAGTTATACAAAGAGACTTCATTGCAGCACTTGTTCTTAATGAACAACCTACATTACATGGCTGAAAAGGTTAAAGGGTCTGAACTTAGGATCATATTCGGAGACGAATGGATCCGAAAACACAACTGGAAGTTTCAGCAGCATGCGATGAAATACGAGAGAGCTAGTTGGAGTTCCATTCTCAACTTTCTTAAGGATGAAGGAATTCACGCTCCTGGTTCAAATTCTGTCTCAAAAACTCTTCTAAAGGAGAAGTTACGAAGCTTCTACCTCGGCTTCGAGGATATTTACAGGATTCAAACAGCTTGGTCGATTCCAGATGTTCAGCTTCGCGAAGATTTGCGGATTTCTATATCGGTTAAAGTGATTCAAGCTTATAGGACATTTGTTGGAAGGAATAATAGTCATGTGAGTGATAAACATATTAGATACACTGCTGATGATTTAGAAAATTATCTTTTGGATTTCTTTGAAGGTTCTCAAAAGTTGTTGCAAAATCCTATTAGGAGATGA

Protein sequence

>MS.gene069413.t1

MNYLRTLTDYSEALNHLLKDQEEEDSISSSPDTSPGTEDDNRSQASPGRFPSMARQFLSFASVLENNLEEKSKLYKETSLQHLFLMNNLHYMAEKVKGSELRIIFGDEWIRKHNWKFQQHAMKYERASWSSILNFLKDEGIHAPGSNSVSKTLLKEKLRSFYLGFEDIYRIQTAWSIPDVQLREDLRISISVKVIQAYRTFVGRNNSHVSDKHIRYTADDLENYLLDFFEGSQKLLQNPIRR