Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069414.t1 | XP_024626193.1 | 98.3 | 59 | 1 | 0 | 2 | 60 | 105 | 163 | 1.00E-23 | 118.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069414.t1 | A0A072TXQ3 | 98.3 | 59 | 1 | 0 | 2 | 60 | 105 | 163 | 7.3e-24 | 118.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene069414 | 0.813038 | 3.13E-51 | -1.69E-46 |
| MS.gene050941 | MS.gene069414 | 0.802507 | 5.43E-49 | -1.69E-46 |
| MS.gene05350 | MS.gene069414 | 0.81007 | 1.38E-50 | -1.69E-46 |
| MS.gene055864 | MS.gene069414 | 0.834678 | 2.61E-56 | -1.69E-46 |
| MS.gene055926 | MS.gene069414 | 0.800794 | 1.22E-48 | -1.69E-46 |
| MS.gene057915 | MS.gene069414 | 0.809945 | 1.47E-50 | -1.69E-46 |
| MS.gene058081 | MS.gene069414 | 0.81484 | 1.26E-51 | -1.69E-46 |
| MS.gene06052 | MS.gene069414 | 0.819098 | 1.39E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069414.t1 | MTR_7g013770 | 98.305 | 59 | 1 | 0 | 2 | 60 | 105 | 163 | 2.34e-34 | 122 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAGTATAAGTTTTTGCAGA+GGG | - | 51466:5009-5028 | MS.gene069414:CDS | 30.0% |
| !! | AAGAGTATAAGTTTTTGCAG+AGG | - | 51466:5008-5027 | MS.gene069414:CDS | 30.0% |
| AAATACTCAGACGTTTCTTC+AGG | + | 51466:4920-4939 | MS.gene069413:intergenic | 35.0% | |
| AGGAAGATCAATCGATGATT+TGG | - | 51466:4885-4904 | MS.gene069414:CDS | 35.0% | |
| ATATTGATCGAGAAGTTGGA+AGG | - | 51466:4962-4981 | MS.gene069414:CDS | 35.0% | |
| GGAAGATCAATCGATGATTT+GGG | - | 51466:4886-4905 | MS.gene069414:CDS | 35.0% | |
| ! | CAATCGATGATTTGGGATTT+GGG | - | 51466:4893-4912 | MS.gene069414:CDS | 35.0% |
| ! | TCAATCGATGATTTGGGATT+TGG | - | 51466:4892-4911 | MS.gene069414:CDS | 35.0% |
| GGCTTATAGTGTTCTTCAGA+CGG | - | 51466:5030-5049 | MS.gene069414:CDS | 40.0% | |
| GCGCATATTGATCGAGAAGT+TGG | - | 51466:4958-4977 | MS.gene069414:CDS | 45.0% | |
| TCTTCAGACGGCTATGACCA+CGG | - | 51466:5042-5061 | MS.gene069414:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 51466 | gene | 4881 | 5069 | 4881 | ID=MS.gene069414 |
| 51466 | mRNA | 4881 | 5069 | 4881 | ID=MS.gene069414.t1;Parent=MS.gene069414 |
| 51466 | exon | 4881 | 5069 | 4881 | ID=MS.gene069414.t1.exon1;Parent=MS.gene069414.t1 |
| 51466 | CDS | 4881 | 5069 | 4881 | ID=cds.MS.gene069414.t1;Parent=MS.gene069414.t1 |
| Gene Sequence |
| Protein sequence |
