Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069441.t1 | XP_003620169.1 | 97.5 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 9.40E-111 | 409.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069441.t1 | Q9LMU2 | 48.3 | 207 | 88 | 8 | 3 | 203 | 2 | 195 | 1.6e-42 | 174.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069441.t1 | G7KKP1 | 97.5 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 6.8e-111 | 409.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052016 | MS.gene069441 | 0.8043 | 2.31E-49 | -1.69E-46 |
| MS.gene05221 | MS.gene069441 | 0.923539 | 2.32E-89 | -1.69E-46 |
| MS.gene05224 | MS.gene069441 | 0.912303 | 2.27E-83 | -1.69E-46 |
| MS.gene052463 | MS.gene069441 | 0.808596 | 2.87E-50 | -1.69E-46 |
| MS.gene055465 | MS.gene069441 | 0.817537 | 3.13E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene069441 | 0.823353 | 1.45E-53 | -1.69E-46 |
| MS.gene058087 | MS.gene069441 | 0.812522 | 4.06E-51 | -1.69E-46 |
| MS.gene058720 | MS.gene069441 | 0.800976 | 1.12E-48 | -1.69E-46 |
| MS.gene059170 | MS.gene069441 | 0.818224 | 2.19E-52 | -1.69E-46 |
| MS.gene061100 | MS.gene069441 | 0.804442 | 2.16E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069441.t1 | MTR_6g078070 | 97.537 | 203 | 5 | 0 | 1 | 203 | 1 | 203 | 2.00e-147 | 408 |
| MS.gene069441.t1 | MTR_6g078100 | 82.039 | 206 | 33 | 2 | 1 | 203 | 1 | 205 | 1.65e-118 | 335 |
| MS.gene069441.t1 | MTR_6g078290 | 44.444 | 207 | 98 | 8 | 5 | 201 | 11 | 210 | 3.14e-45 | 149 |
| MS.gene069441.t1 | MTR_7g037410 | 41.935 | 186 | 94 | 7 | 23 | 203 | 26 | 202 | 6.37e-37 | 127 |
| MS.gene069441.t1 | MTR_6g078120 | 40.933 | 193 | 99 | 7 | 21 | 203 | 26 | 213 | 3.01e-35 | 124 |
| MS.gene069441.t1 | MTR_6g078140 | 40.415 | 193 | 100 | 7 | 21 | 203 | 26 | 213 | 6.72e-34 | 120 |
| MS.gene069441.t1 | MTR_6g078040 | 66.292 | 89 | 25 | 3 | 93 | 177 | 1 | 88 | 7.11e-32 | 111 |
| MS.gene069441.t1 | MTR_6g078280 | 36.923 | 195 | 104 | 7 | 21 | 203 | 25 | 212 | 2.08e-30 | 111 |
| MS.gene069441.t1 | MTR_6g078250 | 40.000 | 175 | 93 | 7 | 24 | 191 | 25 | 194 | 9.98e-30 | 109 |
| MS.gene069441.t1 | MTR_6g078260 | 32.961 | 179 | 109 | 6 | 26 | 203 | 31 | 199 | 1.62e-23 | 93.2 |
| MS.gene069441.t1 | MTR_3g106585 | 33.014 | 209 | 113 | 9 | 5 | 203 | 6 | 197 | 1.01e-22 | 91.3 |
| MS.gene069441.t1 | MTR_6g065460 | 35.211 | 213 | 119 | 9 | 1 | 203 | 1 | 204 | 2.07e-22 | 90.5 |
| MS.gene069441.t1 | MTR_3g106620 | 32.057 | 209 | 117 | 10 | 5 | 203 | 6 | 199 | 9.42e-20 | 83.6 |
| MS.gene069441.t1 | MTR_2g072980 | 31.188 | 202 | 127 | 6 | 3 | 203 | 9 | 199 | 1.39e-19 | 82.8 |
| MS.gene069441.t1 | MTR_0100s0150 | 31.884 | 207 | 122 | 10 | 4 | 202 | 5 | 200 | 5.04e-19 | 81.3 |
| MS.gene069441.t1 | MTR_2g073010 | 32.039 | 206 | 119 | 8 | 3 | 203 | 9 | 198 | 6.85e-19 | 80.9 |
| MS.gene069441.t1 | MTR_0302s0030 | 30.097 | 206 | 128 | 9 | 4 | 202 | 5 | 201 | 1.49e-17 | 77.4 |
| MS.gene069441.t1 | MTR_6g059650 | 31.720 | 186 | 118 | 7 | 20 | 202 | 22 | 201 | 6.51e-17 | 75.9 |
| MS.gene069441.t1 | MTR_6g059680 | 34.595 | 185 | 114 | 6 | 22 | 203 | 28 | 208 | 6.73e-17 | 76.3 |
| MS.gene069441.t1 | MTR_3g463590 | 30.233 | 172 | 115 | 4 | 18 | 189 | 21 | 187 | 2.68e-16 | 74.3 |
| MS.gene069441.t1 | MTR_0084s0030 | 28.293 | 205 | 131 | 9 | 4 | 202 | 7 | 201 | 2.97e-16 | 73.9 |
| MS.gene069441.t1 | MTR_6g059530 | 31.553 | 206 | 127 | 7 | 1 | 203 | 1 | 195 | 3.57e-16 | 73.9 |
| MS.gene069441.t1 | MTR_6g059730 | 33.333 | 183 | 112 | 6 | 24 | 203 | 29 | 204 | 6.62e-16 | 73.6 |
| MS.gene069441.t1 | MTR_6g059410 | 31.553 | 206 | 126 | 8 | 1 | 203 | 1 | 194 | 1.76e-15 | 72.0 |
| MS.gene069441.t1 | MTR_8g461050 | 32.843 | 204 | 117 | 13 | 10 | 202 | 7 | 201 | 8.36e-15 | 70.1 |
| MS.gene069441.t1 | MTR_6g059760 | 30.622 | 209 | 126 | 9 | 1 | 200 | 1 | 199 | 1.74e-13 | 66.6 |
| MS.gene069441.t1 | MTR_8g060550 | 28.426 | 197 | 122 | 10 | 4 | 190 | 2 | 189 | 2.37e-13 | 66.2 |
| MS.gene069441.t1 | MTR_8g461690 | 28.426 | 197 | 122 | 10 | 4 | 190 | 2 | 189 | 2.37e-13 | 66.2 |
| MS.gene069441.t1 | MTR_6g009563 | 30.693 | 202 | 124 | 9 | 10 | 203 | 10 | 203 | 4.40e-12 | 62.8 |
| MS.gene069441.t1 | MTR_6g009650 | 30.882 | 204 | 127 | 9 | 6 | 203 | 7 | 202 | 4.65e-12 | 62.8 |
| MS.gene069441.t1 | MTR_6g066210 | 28.846 | 208 | 132 | 8 | 5 | 203 | 4 | 204 | 5.86e-12 | 62.8 |
| MS.gene069441.t1 | MTR_6g017225 | 30.435 | 161 | 102 | 6 | 6 | 161 | 7 | 162 | 9.58e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069441.t1 | AT1G17860 | 48.309 | 207 | 88 | 8 | 3 | 203 | 2 | 195 | 2.36e-54 | 172 |
| MS.gene069441.t1 | AT1G73260 | 37.017 | 181 | 106 | 5 | 26 | 203 | 30 | 205 | 2.93e-29 | 108 |
| MS.gene069441.t1 | AT1G73325 | 33.659 | 205 | 120 | 7 | 4 | 203 | 10 | 203 | 4.38e-23 | 92.8 |
Find 39 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTAGGGTTGTGTTTAAA+AGG | 0.160681 | 6.4:-52703094 | MS.gene069441:CDS |
| GTGATCAAATTCCATCATTT+AGG | 0.169249 | 6.4:-52703109 | MS.gene069441:CDS |
| ACACCTTGTTTAGCATTATA+AGG | 0.259304 | 6.4:+52703422 | None:intergenic |
| GTATGGCAACTGTTGGTAAT+TGG | 0.271386 | 6.4:-52703262 | MS.gene069441:CDS |
| GAACCAGTTCTTGACATTTC+AGG | 0.287884 | 6.4:-52703608 | MS.gene069441:CDS |
| GCTTCTCTTGAACAACATAA+AGG | 0.342977 | 6.4:+52703480 | None:intergenic |
| TGATCAAATTCCATCATTTA+GGG | 0.346172 | 6.4:-52703108 | MS.gene069441:CDS |
| CAAACTTTGTGTAGGGAACT+AGG | 0.366708 | 6.4:-52703173 | MS.gene069441:CDS |
| CTTCTCTTGAACAACATAAA+GGG | 0.369437 | 6.4:+52703481 | None:intergenic |
| AGGGTTGTTTGTTGATGATA+AGG | 0.370874 | 6.4:-52703153 | MS.gene069441:CDS |
| AATGTCCTCAAACACAAGTT+TGG | 0.377880 | 6.4:-52703349 | MS.gene069441:CDS |
| TTACCAACAGTTGCCATACT+TGG | 0.386300 | 6.4:+52703266 | None:intergenic |
| CAACATAAAGGGGACATGTT+TGG | 0.398228 | 6.4:+52703492 | None:intergenic |
| AGCTTCCAAACTTGTGTTTG+AGG | 0.408512 | 6.4:+52703344 | None:intergenic |
| GGGTTGTTTGTTGATGATAA+GGG | 0.441383 | 6.4:-52703152 | MS.gene069441:CDS |
| TTAAACACAACCCTAAATGA+TGG | 0.447080 | 6.4:+52703098 | None:intergenic |
| TGTAGAAGGTAATCCAAGTA+TGG | 0.452203 | 6.4:-52703279 | MS.gene069441:CDS |
| ATTTAGGGTTGTGTTTAAAA+GGG | 0.479397 | 6.4:-52703093 | MS.gene069441:CDS |
| GAAATGTCAAGAACTGGTTC+AGG | 0.479615 | 6.4:+52703611 | None:intergenic |
| TGACATTTGCAAGCTTCAGC+AGG | 0.493041 | 6.4:+52703194 | None:intergenic |
| AAGCTTCTTAAAGAGTTGAC+AGG | 0.493248 | 6.4:-52703326 | MS.gene069441:CDS |
| GCAAATGTCAAACTTTGTGT+AGG | 0.501531 | 6.4:-52703181 | MS.gene069441:CDS |
| TCAGGCAAACAAGTGACAAC+TGG | 0.504943 | 6.4:-52703590 | MS.gene069441:CDS |
| TTGCCTGAAATGTCAAGAAC+TGG | 0.505604 | 6.4:+52703605 | None:intergenic |
| CAAATGTCAAACTTTGTGTA+GGG | 0.511038 | 6.4:-52703180 | MS.gene069441:CDS |
| TTACCAGTCATAAGAGGTAA+AGG | 0.515215 | 6.4:-52703551 | MS.gene069441:CDS |
| TTAAAGAGTTGACAGGGGTG+TGG | 0.531517 | 6.4:-52703319 | MS.gene069441:CDS |
| GAGAAGCTTGAAGTAAAGAA+TGG | 0.531990 | 6.4:-52703464 | MS.gene069441:CDS |
| AAACTTTGTGTAGGGAACTA+GGG | 0.532296 | 6.4:-52703172 | MS.gene069441:CDS |
| GTCATAAGAGGTAAAGGTGG+TGG | 0.556783 | 6.4:-52703545 | MS.gene069441:CDS |
| CCACCTTTACCTCTTATGAC+TGG | 0.557512 | 6.4:+52703548 | None:intergenic |
| AGCTTCTTAAAGAGTTGACA+GGG | 0.566536 | 6.4:-52703325 | MS.gene069441:CDS |
| TTCTCTTGAACAACATAAAG+GGG | 0.573118 | 6.4:+52703482 | None:intergenic |
| AATCCAAGTATGGCAACTGT+TGG | 0.585675 | 6.4:-52703269 | MS.gene069441:CDS |
| GGTGGAAAATGCTAAGAGTG+AGG | 0.614319 | 6.4:+52703670 | None:intergenic |
| ACACCTTATAATGCTAAACA+AGG | 0.651507 | 6.4:-52703425 | MS.gene069441:CDS |
| CCAGTCATAAGAGGTAAAGG+TGG | 0.660270 | 6.4:-52703548 | MS.gene069441:CDS |
| GGTTTAACAGTTGCAAACCA+TGG | 0.676406 | 6.4:-52703524 | MS.gene069441:CDS |
| GCTTCTTAAAGAGTTGACAG+GGG | 0.746376 | 6.4:-52703324 | MS.gene069441:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACATTTAGTTTTTGTTACAA+AGG | + | chr6.4:52703401-52703420 | None:intergenic | 20.0% |
| ! | TGATCAAATTCCATCATTTA+GGG | - | chr6.4:52703657-52703676 | MS.gene069441:CDS | 25.0% |
| !! | ATTTAGGGTTGTGTTTAAAA+GGG | - | chr6.4:52703672-52703691 | MS.gene069441:CDS | 25.0% |
| !! | TATATTTTACCAGTCATAAG+AGG | - | chr6.4:52703208-52703227 | MS.gene069441:CDS | 25.0% |
| ACACCTTATAATGCTAAACA+AGG | - | chr6.4:52703340-52703359 | MS.gene069441:CDS | 30.0% | |
| CAAATGTCAAACTTTGTGTA+GGG | - | chr6.4:52703585-52703604 | MS.gene069441:CDS | 30.0% | |
| CTTCTCTTGAACAACATAAA+GGG | + | chr6.4:52703287-52703306 | None:intergenic | 30.0% | |
| GTGATCAAATTCCATCATTT+AGG | - | chr6.4:52703656-52703675 | MS.gene069441:CDS | 30.0% | |
| TTAAACACAACCCTAAATGA+TGG | + | chr6.4:52703670-52703689 | None:intergenic | 30.0% | |
| TTCTCTTGAACAACATAAAG+GGG | + | chr6.4:52703286-52703305 | None:intergenic | 30.0% | |
| ! | ACACCTTGTTTAGCATTATA+AGG | + | chr6.4:52703346-52703365 | None:intergenic | 30.0% |
| ! | CATTTAGGGTTGTGTTTAAA+AGG | - | chr6.4:52703671-52703690 | MS.gene069441:CDS | 30.0% |
| !! | TGCAATAGTTTTGCAAATGA+AGG | + | chr6.4:52703131-52703150 | None:intergenic | 30.0% |
| AAACTTTGTGTAGGGAACTA+GGG | - | chr6.4:52703593-52703612 | MS.gene069441:CDS | 35.0% | |
| AAGCTTCTTAAAGAGTTGAC+AGG | - | chr6.4:52703439-52703458 | MS.gene069441:CDS | 35.0% | |
| AATGAAGGCTAAAAAGATGG+TGG | + | chr6.4:52703116-52703135 | None:intergenic | 35.0% | |
| AATGTCCTCAAACACAAGTT+TGG | - | chr6.4:52703416-52703435 | MS.gene069441:CDS | 35.0% | |
| AGCTTCTTAAAGAGTTGACA+GGG | - | chr6.4:52703440-52703459 | MS.gene069441:CDS | 35.0% | |
| GAGAAGCTTGAAGTAAAGAA+TGG | - | chr6.4:52703301-52703320 | MS.gene069441:CDS | 35.0% | |
| GCAAATGAAGGCTAAAAAGA+TGG | + | chr6.4:52703119-52703138 | None:intergenic | 35.0% | |
| GCAAATGTCAAACTTTGTGT+AGG | - | chr6.4:52703584-52703603 | MS.gene069441:CDS | 35.0% | |
| GCTTCTCTTGAACAACATAA+AGG | + | chr6.4:52703288-52703307 | None:intergenic | 35.0% | |
| TGTAGAAGGTAATCCAAGTA+TGG | - | chr6.4:52703486-52703505 | MS.gene069441:CDS | 35.0% | |
| TTACCAGTCATAAGAGGTAA+AGG | - | chr6.4:52703214-52703233 | MS.gene069441:CDS | 35.0% | |
| !! | AGGGTTGTTTGTTGATGATA+AGG | - | chr6.4:52703612-52703631 | MS.gene069441:CDS | 35.0% |
| !! | ATGTTTGGTTGTTTTCACCA+TGG | + | chr6.4:52703261-52703280 | None:intergenic | 35.0% |
| !! | GGGTTGTTTGTTGATGATAA+GGG | - | chr6.4:52703613-52703632 | MS.gene069441:CDS | 35.0% |
| AATCCAAGTATGGCAACTGT+TGG | - | chr6.4:52703496-52703515 | MS.gene069441:CDS | 40.0% | |
| AGCTTCCAAACTTGTGTTTG+AGG | + | chr6.4:52703424-52703443 | None:intergenic | 40.0% | |
| CAAACTTTGTGTAGGGAACT+AGG | - | chr6.4:52703592-52703611 | MS.gene069441:CDS | 40.0% | |
| CAACATAAAGGGGACATGTT+TGG | + | chr6.4:52703276-52703295 | None:intergenic | 40.0% | |
| GAACCAGTTCTTGACATTTC+AGG | - | chr6.4:52703157-52703176 | MS.gene069441:CDS | 40.0% | |
| GCTTCTTAAAGAGTTGACAG+GGG | - | chr6.4:52703441-52703460 | MS.gene069441:CDS | 40.0% | |
| GGTTTAACAGTTGCAAACCA+TGG | - | chr6.4:52703241-52703260 | MS.gene069441:CDS | 40.0% | |
| TTACCAACAGTTGCCATACT+TGG | + | chr6.4:52703502-52703521 | None:intergenic | 40.0% | |
| TTGCCTGAAATGTCAAGAAC+TGG | + | chr6.4:52703163-52703182 | None:intergenic | 40.0% | |
| ! | GAAATGTCAAGAACTGGTTC+AGG | + | chr6.4:52703157-52703176 | None:intergenic | 40.0% |
| !! | GTATGGCAACTGTTGGTAAT+TGG | - | chr6.4:52703503-52703522 | MS.gene069441:CDS | 40.0% |
| CCACCTTTACCTCTTATGAC+TGG | + | chr6.4:52703220-52703239 | None:intergenic | 45.0% | |
| CCAGTCATAAGAGGTAAAGG+TGG | - | chr6.4:52703217-52703236 | MS.gene069441:CDS | 45.0% | |
| GGTGGAAAATGCTAAGAGTG+AGG | + | chr6.4:52703098-52703117 | None:intergenic | 45.0% | |
| GTCATAAGAGGTAAAGGTGG+TGG | - | chr6.4:52703220-52703239 | MS.gene069441:CDS | 45.0% | |
| TCAGGCAAACAAGTGACAAC+TGG | - | chr6.4:52703175-52703194 | MS.gene069441:CDS | 45.0% | |
| TGACATTTGCAAGCTTCAGC+AGG | + | chr6.4:52703574-52703593 | None:intergenic | 45.0% | |
| TTAAAGAGTTGACAGGGGTG+TGG | - | chr6.4:52703446-52703465 | MS.gene069441:CDS | 45.0% | |
| !!! | GGGTGTGGTTTTTAGCTACA+GGG | - | chr6.4:52703461-52703480 | MS.gene069441:CDS | 45.0% |
| !!! | GGTGTGGTTTTTAGCTACAG+GGG | - | chr6.4:52703462-52703481 | MS.gene069441:CDS | 45.0% |
| !!! | GTGTGGTTTTTAGCTACAGG+GGG | - | chr6.4:52703463-52703482 | MS.gene069441:CDS | 45.0% |
| ! | TTAGCTACAGGGGGTGTAGA+AGG | - | chr6.4:52703472-52703491 | MS.gene069441:CDS | 50.0% |
| !!! | GGGGTGTGGTTTTTAGCTAC+AGG | - | chr6.4:52703460-52703479 | MS.gene069441:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 52703088 | 52703699 | 52703088 | ID=MS.gene069441 |
| chr6.4 | mRNA | 52703088 | 52703699 | 52703088 | ID=MS.gene069441.t1;Parent=MS.gene069441 |
| chr6.4 | exon | 52703088 | 52703699 | 52703088 | ID=MS.gene069441.t1.exon1;Parent=MS.gene069441.t1 |
| chr6.4 | CDS | 52703088 | 52703699 | 52703088 | ID=cds.MS.gene069441.t1;Parent=MS.gene069441.t1 |
| Gene Sequence |
| Protein sequence |
