AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069664

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069664.t1 MTR_5g007630 73.469 245 43 5 1 224 498 741 1.84e-110 333
MS.gene069664.t1 MTR_6g075440 62.550 251 56 2 1 222 503 744 9.26e-98 301
MS.gene069664.t1 MTR_6g075440 62.550 251 56 2 1 222 503 744 1.47e-97 300
MS.gene069664.t1 MTR_6g076060 65.021 243 65 3 1 223 231 473 3.88e-91 277
MS.gene069664.t1 MTR_6g477760 58.750 240 69 4 1 216 464 697 1.80e-80 254
MS.gene069664.t1 MTR_6g075290 58.943 246 71 4 1 222 327 566 2.04e-79 248
MS.gene069664.t1 MTR_6g075290 58.943 246 71 4 1 222 488 727 6.59e-78 248
MS.gene069664.t1 MTR_6g075290 58.943 246 71 4 1 222 529 768 1.59e-77 248
MS.gene069664.t1 MTR_6g075410 56.735 245 65 5 1 216 432 664 4.12e-77 244
MS.gene069664.t1 MTR_6g075390 78.912 147 31 0 4 150 449 595 4.02e-75 239
MS.gene069664.t1 MTR_3g088875 36.771 223 123 5 3 209 379 599 5.23e-35 131
MS.gene069664.t1 MTR_3g109030 35.565 239 136 6 2 224 416 652 8.35e-35 130
MS.gene069664.t1 MTR_2g460590 37.168 226 124 5 2 211 386 609 8.50e-35 130
MS.gene069664.t1 MTR_4g130820 36.245 229 127 5 1 212 437 663 3.34e-34 129
MS.gene069664.t1 MTR_8g027755 36.123 227 125 7 1 209 424 648 7.67e-34 128
MS.gene069664.t1 MTR_7g117310 36.207 232 126 8 1 213 420 648 8.45e-32 122
MS.gene069664.t1 MTR_7g117310 36.207 232 126 8 1 213 366 594 8.57e-32 122
MS.gene069664.t1 MTR_7g117310 36.207 232 126 8 1 213 432 660 1.03e-31 122
MS.gene069664.t1 MTR_3g091080 36.522 230 127 6 1 213 415 642 2.04e-31 121
MS.gene069664.t1 MTR_1g028370 34.783 230 131 6 1 213 417 644 2.35e-31 121
MS.gene069664.t1 MTR_7g034605 35.426 223 126 6 3 209 387 607 2.06e-30 118
MS.gene069664.t1 MTR_1g064240 35.294 238 117 7 2 214 406 631 1.02e-29 116
MS.gene069664.t1 MTR_2g081110 33.190 232 138 6 1 217 402 631 7.03e-29 114
MS.gene069664.t1 MTR_4g058730 35.294 221 111 6 7 204 399 610 1.15e-28 113
MS.gene069664.t1 MTR_8g464570 34.052 232 136 6 1 217 406 635 1.33e-28 113
MS.gene069664.t1 MTR_2g094860 36.486 222 117 7 7 209 417 633 1.35e-28 113
MS.gene069664.t1 MTR_7g012260 31.513 238 138 7 1 219 454 685 1.68e-23 98.6
MS.gene069664.t1 MTR_6g075460 82.051 39 7 0 185 223 87 125 3.88e-14 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069664.t1 AT3G17690 50.202 247 83 4 1 219 492 726 1.25e-70 229
MS.gene069664.t1 AT3G17700 51.724 232 72 4 1 203 525 745 3.00e-66 218
MS.gene069664.t1 AT3G17700 65.217 161 54 1 1 161 525 683 1.78e-65 214
MS.gene069664.t1 AT3G17690 65.132 152 49 2 1 152 492 639 2.75e-60 200
MS.gene069664.t1 AT4G30360 37.662 231 126 4 2 216 413 641 5.36e-38 140
MS.gene069664.t1 AT4G30560 38.938 226 119 5 1 209 444 667 2.38e-35 132
MS.gene069664.t1 AT4G30560 38.938 226 119 5 1 209 444 667 2.38e-35 132
MS.gene069664.t1 AT4G30560 38.938 226 119 5 1 209 418 641 2.41e-35 132
MS.gene069664.t1 AT4G30560 38.938 226 119 5 1 209 418 641 2.41e-35 132
MS.gene069664.t1 AT4G30560 38.938 226 119 5 1 209 418 641 2.41e-35 132
MS.gene069664.t1 AT2G24610 36.681 229 126 5 2 213 413 639 6.04e-35 131
MS.gene069664.t1 AT1G19780 35.556 225 127 4 1 209 228 450 6.81e-35 130
MS.gene069664.t1 AT2G24610 35.294 238 135 5 2 222 428 663 7.91e-35 131
MS.gene069664.t1 AT1G19780 35.556 225 127 4 1 209 439 661 1.85e-34 130
MS.gene069664.t1 AT1G15990 35.111 225 128 4 1 209 433 655 9.15e-34 128
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 418 640 1.02e-33 127
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 445 667 1.19e-33 127
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 445 667 1.19e-33 127
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 445 667 1.19e-33 127
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 445 667 1.19e-33 127
MS.gene069664.t1 AT2G23980 37.333 225 123 4 1 209 445 667 1.19e-33 127
MS.gene069664.t1 AT2G23980 45.513 156 76 3 1 149 468 621 1.52e-33 127
MS.gene069664.t1 AT2G23980 45.513 156 76 3 1 149 468 621 1.52e-33 127
MS.gene069664.t1 AT2G23980 45.513 156 76 3 1 149 468 621 1.52e-33 127
MS.gene069664.t1 AT2G23980 45.513 156 76 3 1 149 468 621 1.52e-33 127
MS.gene069664.t1 AT1G01340 35.146 239 118 8 1 213 404 631 4.98e-33 125
MS.gene069664.t1 AT1G01340 35.146 239 118 8 1 213 399 626 5.09e-33 125
MS.gene069664.t1 AT5G57940 36.444 225 125 4 1 209 429 651 7.27e-33 125
MS.gene069664.t1 AT5G57940 36.444 225 125 4 1 209 429 651 7.27e-33 125
MS.gene069664.t1 AT5G57940 44.156 154 77 3 1 147 422 573 7.41e-33 125
MS.gene069664.t1 AT5G53130 35.455 220 124 4 1 204 417 634 1.67e-32 124
MS.gene069664.t1 AT4G01010 33.051 236 139 6 1 219 405 638 6.23e-32 122
MS.gene069664.t1 AT4G01010 33.051 236 139 6 1 219 405 638 6.23e-32 122
MS.gene069664.t1 AT3G48010 34.413 247 103 7 2 211 389 613 3.68e-31 120
MS.gene069664.t1 AT2G28260 37.445 227 118 6 2 209 363 584 6.15e-31 119
MS.gene069664.t1 AT2G28260 37.445 227 118 6 2 209 403 624 6.63e-31 119
MS.gene069664.t1 AT5G54250 33.621 232 137 6 1 217 427 656 6.34e-30 117
MS.gene069664.t1 AT5G54250 33.621 232 137 6 1 217 427 656 6.34e-30 117
MS.gene069664.t1 AT5G54250 33.621 232 137 6 1 217 427 656 6.34e-30 117
MS.gene069664.t1 AT5G14870 34.802 227 123 6 6 212 385 606 8.03e-30 116
MS.gene069664.t1 AT2G46430 35.510 245 128 10 1 219 408 648 1.62e-28 113
MS.gene069664.t1 AT2G46430 35.510 245 128 10 1 219 408 648 1.62e-28 113
MS.gene069664.t1 AT5G54250 33.333 231 134 7 1 217 427 651 5.74e-27 108
MS.gene069664.t1 AT5G15410 33.333 225 119 7 1 203 462 677 1.52e-22 95.9
MS.gene069664.t1 AT5G15410 33.333 225 119 7 1 203 461 676 1.54e-22 95.9
MS.gene069664.t1 AT2G46450 30.000 230 146 5 1 218 343 569 1.13e-21 93.2
MS.gene069664.t1 AT2G46450 30.000 230 146 5 1 218 330 556 1.32e-21 93.2
MS.gene069664.t1 AT2G46450 30.000 230 146 5 1 218 366 592 1.36e-21 93.2
MS.gene069664.t1 AT2G46450 30.000 230 146 5 1 218 366 592 1.36e-21 93.2
MS.gene069664.t1 AT2G46440 32.627 236 126 9 1 213 188 413 1.46e-19 86.7
MS.gene069664.t1 AT2G46440 32.271 251 128 10 1 219 366 606 3.79e-19 85.9

Find 53 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTATTGTGCGTGGAAAAT+TGG 0.274539 6.1:+67261166 MS.gene069664:CDS
GCTTACCAGAAGAACTTAAA+AGG 0.284129 6.1:+67260462 MS.gene069664:CDS
CACTTGCAGTAAACAGAATT+CGG 0.289321 6.1:+67262104 MS.gene069664:CDS
ATGTCTGTCTGGAGATCTTC+TGG 0.319596 6.1:-67260676 None:intergenic
AAGGGGTGTTCCTGAGAAAA+TGG 0.334550 6.1:+67260639 MS.gene069664:CDS
ATACAGACGACATACATTAA+AGG 0.335319 6.1:+67261101 MS.gene069664:CDS
TTAGGTATGAATCACCTTAC+TGG 0.364609 6.1:+67262077 MS.gene069664:intron
TCACGAATGGCGTCTAAGAT+AGG 0.365376 6.1:-67261071 None:intergenic
AGGAAGTAAATTTCTCAGTC+AGG 0.403042 6.1:+67261121 MS.gene069664:CDS
TGTTCCTGAGAAAATGGTTC+TGG 0.409988 6.1:+67260645 MS.gene069664:CDS
GTTCTTCTGGTAAGCAATGA+TGG 0.420523 6.1:-67260454 None:intergenic
CGTCGGAGAGGATGGAATTC+CGG 0.422155 6.1:+67261193 MS.gene069664:CDS
TTCGGGTTGCATGGAGATAC+AGG 0.422208 6.1:+67262122 MS.gene069664:CDS
TAGGGTCATCCTCAGCCATA+TGG 0.436659 6.1:-67261052 None:intergenic
GGCAGGCTGAACGGTATAGT+TGG 0.446424 6.1:+67260608 MS.gene069664:CDS
TCCCCTTCTGATAACGGAAC+CGG 0.459445 6.1:-67261212 None:intergenic
GAAACCACATCGATTAATCA+TGG 0.466495 6.1:-67261360 None:intergenic
GCAGGCTGAACGGTATAGTT+GGG 0.472885 6.1:+67260609 MS.gene069664:CDS
TTGGAGAGCGTCGGAGAGGA+TGG 0.482934 6.1:+67261185 MS.gene069664:CDS
GTCATGCAGGAGAGTGCGGC+AGG 0.485642 6.1:+67260591 MS.gene069664:intron
TATTAACAAATTTGAAGAGA+TGG 0.501093 6.1:-67260705 None:intergenic
GTATAGTTGGGCTGCAACAA+GGG 0.505727 6.1:+67260621 MS.gene069664:CDS
CAAGCATCCCCTTCTGATAA+CGG 0.505912 6.1:-67261218 None:intergenic
GATGGCGTCTTATGTCTGTC+TGG 0.521052 6.1:-67260687 None:intergenic
ACTTGCAGTAAACAGAATTC+GGG 0.523626 6.1:+67262105 MS.gene069664:CDS
TAAACAGAATTCGGGTTGCA+TGG 0.527023 6.1:+67262113 MS.gene069664:CDS
GGTATAGTTGGGCTGCAACA+AGG 0.533253 6.1:+67260620 MS.gene069664:CDS
CACGAATGGCGTCTAAGATA+GGG 0.533961 6.1:-67261070 None:intergenic
CATCGATTAATCATGGTTTG+AGG 0.537968 6.1:-67261353 None:intergenic
AGGATAGAAATGCAACTTAG+AGG 0.538796 6.1:+67260407 None:intergenic
GGGTGGTCTTGTACAGAAGA+TGG 0.547676 6.1:+67261142 MS.gene069664:CDS
GGAAGTAAATTTCTCAGTCA+GGG 0.548088 6.1:+67261122 MS.gene069664:CDS
TGTTTGATAATCATTATATG+TGG 0.550337 6.1:-67262170 None:intergenic
AGGGTCATCCTCAGCCATAT+GGG 0.559056 6.1:-67261051 None:intergenic
TCCGGTTCCGTTATCAGAAG+GGG 0.560727 6.1:+67261211 MS.gene069664:CDS
GGAGAGTGCGGCAGGCTGAA+CGG 0.561282 6.1:+67260599 MS.gene069664:intron
ACTGCAAGTGATCTCCAGTA+AGG 0.565458 6.1:-67262091 None:intergenic
ATTCCGGTTCCGTTATCAGA+AGG 0.567052 6.1:+67261209 MS.gene069664:CDS
TTATCAAACATTGATGCCAT+AGG 0.570396 6.1:+67262183 MS.gene069664:CDS
CTGTATTAGTCTCTCACGAA+TGG 0.579807 6.1:-67261084 None:intergenic
GATGTGGTTTCTCTTGCAGA+AGG 0.583374 6.1:+67261372 MS.gene069664:CDS
ATCTGTCATGCAGGAGAGTG+CGG 0.584547 6.1:+67260587 MS.gene069664:intron
GTGGTGAAGAACTTCTAAGA+TGG 0.591370 6.1:+67261240 MS.gene069664:CDS
TTCCGGTTCCGTTATCAGAA+GGG 0.604864 6.1:+67261210 MS.gene069664:CDS
AAGATGGTGTTTATTGTGCG+TGG 0.607353 6.1:+67261158 MS.gene069664:CDS
TATAGTTGGGCTGCAACAAG+GGG 0.617263 6.1:+67260622 MS.gene069664:CDS
CGTGGAAAATTGGAGAGCGT+CGG 0.618147 6.1:+67261176 MS.gene069664:CDS
GAACAGTCTTCTGAAAGCAA+AGG 0.627118 6.1:+67261269 MS.gene069664:CDS
TTATCAGAAGGGGATGCTTG+TGG 0.631551 6.1:+67261221 MS.gene069664:CDS
CAAACCATGATTAATCGATG+TGG 0.644618 6.1:+67261356 MS.gene069664:CDS
GAGGTCGTGATGTTGAGCAA+TGG 0.676062 6.1:+67260426 MS.gene069664:CDS
AAAATTGGAGAGCGTCGGAG+AGG 0.681877 6.1:+67261181 MS.gene069664:CDS
AGTAAATTTCTCAGTCAGGG+TGG 0.692049 6.1:+67261125 MS.gene069664:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATAATGGATTACTTATTA+TGG + chr6.1:67260543-67260562 MS.gene069664:intron 15.0%
!! AAAACACATCTATCAAATAA+TGG + chr6.1:67260529-67260548 MS.gene069664:intron 20.0%
!! AAATGCAATTGAATCATAAA+AGG - chr6.1:67261624-67261643 None:intergenic 20.0%
!! AATGCAATTGAATCATAAAA+GGG - chr6.1:67261623-67261642 None:intergenic 20.0%
!! TATTAACAAATTTGAAGAGA+TGG - chr6.1:67260708-67260727 None:intergenic 20.0%
!! TTTATGATTCAATTGCATTT+CGG + chr6.1:67261624-67261643 MS.gene069664:intron 20.0%
!!! ATTATTTGATAGATGTGTTT+TGG - chr6.1:67260530-67260549 None:intergenic 20.0%
!!! TGGTTTTGATAATAGATATT+AGG - chr6.1:67262017-67262036 None:intergenic 20.0%
!!! TGTTTGATAATCATTATATG+TGG - chr6.1:67262173-67262192 None:intergenic 20.0%
!!! TTAAGTCGATTTTATTTATC+TGG + chr6.1:67260763-67260782 MS.gene069664:intron 20.0%
! AATTGTTATGAGTTCCATAT+TGG - chr6.1:67261954-67261973 None:intergenic 25.0%
! ACTTATTATGGTACAAATGA+TGG + chr6.1:67260555-67260574 MS.gene069664:intron 25.0%
! AGAATGTGTTTATAAGTGAT+GGG - chr6.1:67261710-67261729 None:intergenic 25.0%
! ATATCTTATCTCATCCAATA+TGG + chr6.1:67261937-67261956 MS.gene069664:intron 25.0%
! TAAAACAATGACGCATTAAA+AGG + chr6.1:67260940-67260959 MS.gene069664:intron 25.0%
! TATGACAATATGTATTCAAG+TGG - chr6.1:67261920-67261939 None:intergenic 25.0%
! TCTCTGACGTAATAATTATT+TGG + chr6.1:67262039-67262058 MS.gene069664:intron 25.0%
!! TTTTCTCATTCAACTTCTAT+TGG - chr6.1:67260879-67260898 None:intergenic 25.0%
ACAATATGTATTCAAGTGGT+GGG - chr6.1:67261916-67261935 None:intergenic 30.0%
ATACAGACGACATACATTAA+AGG + chr6.1:67261101-67261120 MS.gene069664:CDS 30.0%
ATACTATATATCTGTCATGC+AGG + chr6.1:67260578-67260597 MS.gene069664:intron 30.0%
ATTTGCATAAACCTTTGTCT+TGG - chr6.1:67260908-67260927 None:intergenic 30.0%
GAAAACAATTTCCAAGACAA+AGG + chr6.1:67260894-67260913 MS.gene069664:intron 30.0%
GAGAATGTGTTTATAAGTGA+TGG - chr6.1:67261711-67261730 None:intergenic 30.0%
GCATGTAAATAGCATACTTA+TGG - chr6.1:67261995-67262014 None:intergenic 30.0%
GTGTTCTTAACCTTATAAGA+CGG - chr6.1:67261688-67261707 None:intergenic 30.0%
TAACTCAACCATTAAAACAC+TGG + chr6.1:67261865-67261884 MS.gene069664:intron 30.0%
TAATTATTACGTCAGAGACA+TGG - chr6.1:67262037-67262056 None:intergenic 30.0%
TGATGATCTAACATTTCTAC+AGG + chr6.1:67261016-67261035 MS.gene069664:intron 30.0%
! ACCATCTTAGAATTTGAGTT+AGG + chr6.1:67261840-67261859 MS.gene069664:intron 30.0%
!! CTCAAATTCTAAGATGGTAT+CGG - chr6.1:67261838-67261857 None:intergenic 30.0%
!! TGGTTGTTTTGTGTTATGTT+AGG + chr6.1:67262059-67262078 MS.gene069664:intron 30.0%
!!! AGTGTTTTAATGGTTGAGTT+AGG - chr6.1:67261866-67261885 None:intergenic 30.0%
!!! TCATACCTTTTAAGTTCTTC+TGG - chr6.1:67260470-67260489 None:intergenic 30.0%
AAAAATAAAGCTTCACAGCC+AGG + chr6.1:67261398-67261417 MS.gene069664:intron 35.0%
AAAATAAAGCTTCACAGCCA+GGG + chr6.1:67261399-67261418 MS.gene069664:intron 35.0%
ACTTGCAGTAAACAGAATTC+GGG + chr6.1:67262105-67262124 MS.gene069664:CDS 35.0%
AGGAAGTAAATTTCTCAGTC+AGG + chr6.1:67261121-67261140 MS.gene069664:CDS 35.0%
AGTAAGATGCTTAACCAATG+TGG + chr6.1:67261440-67261459 MS.gene069664:intron 35.0%
ATTGGATTCCAACAAGTTAC+AGG - chr6.1:67260861-67260880 None:intergenic 35.0%
CAAACCATGATTAATCGATG+TGG + chr6.1:67261356-67261375 MS.gene069664:CDS 35.0%
CACTTGCAGTAAACAGAATT+CGG + chr6.1:67262104-67262123 MS.gene069664:CDS 35.0%
GACAATATGTATTCAAGTGG+TGG - chr6.1:67261917-67261936 None:intergenic 35.0%
GCTTACCAGAAGAACTTAAA+AGG + chr6.1:67260462-67260481 MS.gene069664:CDS 35.0%
GGAAGTAAATTTCTCAGTCA+GGG + chr6.1:67261122-67261141 MS.gene069664:CDS 35.0%
GGCGCATGAATATAAATGAT+GGG + chr6.1:67261791-67261810 MS.gene069664:intron 35.0%
GTTTATTGTGCGTGGAAAAT+TGG + chr6.1:67261166-67261185 MS.gene069664:CDS 35.0%
TATTGACACAAAGACAGAAG+GGG - chr6.1:67261584-67261603 None:intergenic 35.0%
TCAGACCTTATCTCTTAAGT+GGG + chr6.1:67261729-67261748 MS.gene069664:intron 35.0%
TTAGGTATGAATCACCTTAC+TGG + chr6.1:67262077-67262096 MS.gene069664:intron 35.0%
TTATAACTCCTTGAACACGA+GGG - chr6.1:67261533-67261552 None:intergenic 35.0%
! CATCGATTAATCATGGTTTG+AGG - chr6.1:67261356-67261375 None:intergenic 35.0%
! CTCTTTTCGCAAGATATTTG+CGG + chr6.1:67261505-67261524 MS.gene069664:intron 35.0%
! GAAACCACATCGATTAATCA+TGG - chr6.1:67261363-67261382 None:intergenic 35.0%
! GCATGAATATAAATGATGGG+TGG + chr6.1:67261794-67261813 MS.gene069664:intron 35.0%
! GCCTAACTCAAATTCTAAGA+TGG - chr6.1:67261844-67261863 None:intergenic 35.0%
! GTATTGACACAAAGACAGAA+GGG - chr6.1:67261585-67261604 None:intergenic 35.0%
! TTTTCCAGAACCATTTTCTC+AGG - chr6.1:67260652-67260671 None:intergenic 35.0%
!! AGTATTGACACAAAGACAGA+AGG - chr6.1:67261586-67261605 None:intergenic 35.0%
!! CATTAAAACACTGGCTTGAT+AGG + chr6.1:67261874-67261893 MS.gene069664:intron 35.0%
!! CTATCAAGCCAGTGTTTTAA+TGG - chr6.1:67261876-67261895 None:intergenic 35.0%
!!! AATTTTTTCCCATATGGCTG+AGG + chr6.1:67261043-67261062 MS.gene069664:CDS 35.0%
!!! GGTTCGAATTTTTTCCCATA+TGG + chr6.1:67261037-67261056 MS.gene069664:intron 35.0%
AAGATGCTTAACCAATGTGG+AGG + chr6.1:67261443-67261462 MS.gene069664:intron 40.0%
AAGATGGTGTTTATTGTGCG+TGG + chr6.1:67261158-67261177 MS.gene069664:CDS 40.0%
ACAATAGATTCGGGCGTATT+AGG - chr6.1:67261767-67261786 None:intergenic 40.0%
AGTAAATTTCTCAGTCAGGG+TGG + chr6.1:67261125-67261144 MS.gene069664:CDS 40.0%
CAAGTCCCACTTAAGAGATA+AGG - chr6.1:67261737-67261756 None:intergenic 40.0%
CGGCGCATGAATATAAATGA+TGG + chr6.1:67261790-67261809 MS.gene069664:intron 40.0%
CTCAGACCTTATCTCTTAAG+TGG + chr6.1:67261728-67261747 MS.gene069664:intron 40.0%
CTCGTGTTCAAGGAGTTATA+AGG + chr6.1:67261532-67261551 MS.gene069664:intron 40.0%
CTTATAACTCCTTGAACACG+AGG - chr6.1:67261534-67261553 None:intergenic 40.0%
CTTATCTCTTAAGTGGGACT+TGG + chr6.1:67261735-67261754 MS.gene069664:intron 40.0%
CTTCTTCAATGTCTTTAGCG+CGG - chr6.1:67261479-67261498 None:intergenic 40.0%
GATGGGTGGTTCAAATCAAT+AGG + chr6.1:67261808-67261827 MS.gene069664:intron 40.0%
GTTCTTCTGGTAAGCAATGA+TGG - chr6.1:67260457-67260476 None:intergenic 40.0%
TGTTCCTGAGAAAATGGTTC+TGG + chr6.1:67260645-67260664 MS.gene069664:CDS 40.0%
! CTGTATTAGTCTCTCACGAA+TGG - chr6.1:67261087-67261106 None:intergenic 40.0%
! GAACAGTCTTCTGAAAGCAA+AGG + chr6.1:67261269-67261288 MS.gene069664:CDS 40.0%
! GATTGCGACAACTTCTTTTG+AGG - chr6.1:67261325-67261344 None:intergenic 40.0%
! GTGGTGAAGAACTTCTAAGA+TGG + chr6.1:67261240-67261259 MS.gene069664:CDS 40.0%
!! AAAACACTGGCTTGATAGGT+GGG + chr6.1:67261878-67261897 MS.gene069664:intron 40.0%
!! TAAAACACTGGCTTGATAGG+TGG + chr6.1:67261877-67261896 MS.gene069664:intron 40.0%
!! TAAACAGAATTCGGGTTGCA+TGG + chr6.1:67262113-67262132 MS.gene069664:CDS 40.0%
!! TGCATTTCGGTGTTGTGTTT+GGG + chr6.1:67261637-67261656 MS.gene069664:intron 40.0%
!! TTGCATTTCGGTGTTGTGTT+TGG + chr6.1:67261636-67261655 MS.gene069664:intron 40.0%
ACACAAAGACAGAAGGGGAA+TGG - chr6.1:67261579-67261598 None:intergenic 45.0%
ACCTTATAAGACGGCTCTGT+AGG - chr6.1:67261679-67261698 None:intergenic 45.0%
ACGCCCGAATCTATTGTACT+CGG + chr6.1:67261770-67261789 MS.gene069664:intron 45.0%
ACTGCAAGTGATCTCCAGTA+AGG - chr6.1:67262094-67262113 None:intergenic 45.0%
ATGTCTGTCTGGAGATCTTC+TGG - chr6.1:67260679-67260698 None:intergenic 45.0%
ATTCCGGTTCCGTTATCAGA+AGG + chr6.1:67261209-67261228 MS.gene069664:CDS 45.0%
CAAGCATCCCCTTCTGATAA+CGG - chr6.1:67261221-67261240 None:intergenic 45.0%
CACAAAGACAGAAGGGGAAT+GGG - chr6.1:67261578-67261597 None:intergenic 45.0%
CCTTATAAGACGGCTCTGTA+GGG - chr6.1:67261678-67261697 None:intergenic 45.0%
GAGTGAAAATGCCTCCACAT+TGG - chr6.1:67261457-67261476 None:intergenic 45.0%
GATGTGGTTTCTCTTGCAGA+AGG + chr6.1:67261372-67261391 MS.gene069664:CDS 45.0%
GTATAGTTGGGCTGCAACAA+GGG + chr6.1:67260621-67260640 MS.gene069664:CDS 45.0%
TATAGTTGGGCTGCAACAAG+GGG + chr6.1:67260622-67260641 MS.gene069664:CDS 45.0%
TGCGCCGAGTACAATAGATT+CGG - chr6.1:67261777-67261796 None:intergenic 45.0%
TTATCAGAAGGGGATGCTTG+TGG + chr6.1:67261221-67261240 MS.gene069664:CDS 45.0%
TTCCGGTTCCGTTATCAGAA+GGG + chr6.1:67261210-67261229 MS.gene069664:CDS 45.0%
! CACGAATGGCGTCTAAGATA+GGG - chr6.1:67261073-67261092 None:intergenic 45.0%
! TCACGAATGGCGTCTAAGAT+AGG - chr6.1:67261074-67261093 None:intergenic 45.0%
!! AAGGGGTGTTCCTGAGAAAA+TGG + chr6.1:67260639-67260658 MS.gene069664:CDS 45.0%
!! TTCGGTGTTGTGTTTGGGTT+GGG + chr6.1:67261642-67261661 MS.gene069664:intron 45.0%
!! TTTCGGTGTTGTGTTTGGGT+TGG + chr6.1:67261641-67261660 MS.gene069664:intron 45.0%
!!! AATTTTATTTAAAACATTAT+AGG - chr6.1:67260819-67260838 None:intergenic 5.0%
AAGACAGAAGGGGAATGGGA+TGG - chr6.1:67261574-67261593 None:intergenic 50.0%
AGGGTCATCCTCAGCCATAT+GGG - chr6.1:67261054-67261073 None:intergenic 50.0%
ATCTGTCATGCAGGAGAGTG+CGG + chr6.1:67260587-67260606 MS.gene069664:intron 50.0%
CCCTACAGAGCCGTCTTATA+AGG + chr6.1:67261675-67261694 MS.gene069664:intron 50.0%
GAGGTCGTGATGTTGAGCAA+TGG + chr6.1:67260426-67260445 MS.gene069664:CDS 50.0%
GATGGCGTCTTATGTCTGTC+TGG - chr6.1:67260690-67260709 None:intergenic 50.0%
GCAGGCTGAACGGTATAGTT+GGG + chr6.1:67260609-67260628 MS.gene069664:CDS 50.0%
GCGCCGAGTACAATAGATTC+GGG - chr6.1:67261776-67261795 None:intergenic 50.0%
GGTATAGTTGGGCTGCAACA+AGG + chr6.1:67260620-67260639 MS.gene069664:CDS 50.0%
TAGGGTCATCCTCAGCCATA+TGG - chr6.1:67261055-67261074 None:intergenic 50.0%
TCCGGTTCCGTTATCAGAAG+GGG + chr6.1:67261211-67261230 MS.gene069664:CDS 50.0%
TTCTGTCGCTAGTCAAACCC+TGG - chr6.1:67261419-67261438 None:intergenic 50.0%
! CGTGGAAAATTGGAGAGCGT+CGG + chr6.1:67261176-67261195 MS.gene069664:CDS 50.0%
!! AAAATTGGAGAGCGTCGGAG+AGG + chr6.1:67261181-67261200 MS.gene069664:CDS 50.0%
!! GGGTGGTCTTGTACAGAAGA+TGG + chr6.1:67261142-67261161 MS.gene069664:CDS 50.0%
!! TCCCCTTCTGATAACGGAAC+CGG - chr6.1:67261215-67261234 None:intergenic 50.0%
!! TTCGGGTTGCATGGAGATAC+AGG + chr6.1:67262122-67262141 MS.gene069664:CDS 50.0%
AGTTACAGGCAGACGCGTAG+AGG - chr6.1:67260847-67260866 None:intergenic 55.0%
CGTCGGAGAGGATGGAATTC+CGG + chr6.1:67261193-67261212 MS.gene069664:CDS 55.0%
GGCAGGCTGAACGGTATAGT+TGG + chr6.1:67260608-67260627 MS.gene069664:CDS 55.0%
! CGCGTCTGCCTGTAACTTGT+TGG + chr6.1:67260850-67260869 MS.gene069664:intron 55.0%
TTGCGGAGCCCTCGTGTTCA+AGG + chr6.1:67261522-67261541 MS.gene069664:intron 60.0%
!! TTGGAGAGCGTCGGAGAGGA+TGG + chr6.1:67261185-67261204 MS.gene069664:CDS 60.0%
! GGAGAGTGCGGCAGGCTGAA+CGG + chr6.1:67260599-67260618 MS.gene069664:intron 65.0%
! GTCATGCAGGAGAGTGCGGC+AGG + chr6.1:67260591-67260610 MS.gene069664:intron 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 67260416 67262204 67260416 ID=MS.gene069664
chr6.1 mRNA 67260416 67262204 67260416 ID=MS.gene069664.t1;Parent=MS.gene069664
chr6.1 exon 67260416 67260483 67260416 ID=MS.gene069664.t1.exon1;Parent=MS.gene069664.t1
chr6.1 CDS 67260416 67260483 67260416 ID=cds.MS.gene069664.t1;Parent=MS.gene069664.t1
chr6.1 exon 67260600 67260729 67260600 ID=MS.gene069664.t1.exon2;Parent=MS.gene069664.t1
chr6.1 CDS 67260600 67260729 67260600 ID=cds.MS.gene069664.t1;Parent=MS.gene069664.t1
chr6.1 exon 67261038 67261393 67261038 ID=MS.gene069664.t1.exon3;Parent=MS.gene069664.t1
chr6.1 CDS 67261038 67261393 67261038 ID=cds.MS.gene069664.t1;Parent=MS.gene069664.t1
chr6.1 exon 67262081 67262204 67262081 ID=MS.gene069664.t1.exon4;Parent=MS.gene069664.t1
chr6.1 CDS 67262081 67262204 67262081 ID=cds.MS.gene069664.t1;Parent=MS.gene069664.t1
Gene Sequence

>MS.gene069664

ATGCAACTTAGAGGTCGTGATGTTGAGCAATGGATGAACCATCATTGCTTACCAGAAGAACTTAAAAGGTATGAAATGTTCTAATTCTTTTAATGAATCAAATCAACATTGCCAAAACACATCTATCAAATAATGGATTACTTATTATGGTACAAATGATGGATACTATATATCTGTCATGCAGGAGAGTGCGGCAGGCTGAACGGTATAGTTGGGCTGCAACAAGGGGTGTTCCTGAGAAAATGGTTCTGGAAAATTTGCCAGAAGATCTCCAGACAGACATAAGACGCCATCTCTTCAAATTTGTTAATAAAGTATGCATTTAAAAAGAGTTCATGATATACTCTTTAAGTCGATTTTATTTATCTGGTTCAGATGTTATGTGAACTTTAAAATCTCACCTATAATGTTTTAAATAAAATTTGTTTCCTCTACGCGTCTGCCTGTAACTTGTTGGAATCCAATAGAAGTTGAATGAGAAAACAATTTCCAAGACAAAGGTTTATGCAAATTCATAATGAAACTAAAACAATGACGCATTAAAAGGCAATTATATTAAACAAGTTTGAATGCGATTTATGATGATGATTTAATTTTCTATGATGATCTAACATTTCTACAGGTTCGAATTTTTTCCCATATGGCTGAGGATGACCCTATCTTAGACGCCATTCGTGAGAGACTAATACAGACGACATACATTAAAGGAAGTAAATTTCTCAGTCAGGGTGGTCTTGTACAGAAGATGGTGTTTATTGTGCGTGGAAAATTGGAGAGCGTCGGAGAGGATGGAATTCCGGTTCCGTTATCAGAAGGGGATGCTTGTGGTGAAGAACTTCTAAGATGGTATCTTGAACAGTCTTCTGAAAGCAAAGGTACAACTATACTTGATATAATGAGAACATGCCTCAAAAGAAGTTGTCGCAATCACAACATGCCTCAAACCATGATTAATCGATGTGGTTTCTCTTGCAGAAGGTTAAAAAATAAAGCTTCACAGCCAGGGTTTGACTAGCGACAGAACAGTAAGATGCTTAACCAATGTGGAGGCATTTTCACTCCGCGCTAAAGACATTGAAGAAGTCACAACTCTTTTCGCAAGATATTTGCGGAGCCCTCGTGTTCAAGGAGTTATAAGGTCATTTATTTAATCATCCATCCCATTCCCCTTCTGTCTTTGTGTCAATACTTCAATATATCTTCACCCTTTTATGATTCAATTGCATTTCGGTGTTGTGTTTGGGTTGGGCTTAACTCAACCCTACAGAGCCGTCTTATAAGGTTAAGAACACCCATCACTTATAAACACATTCTCAGACCTTATCTCTTAAGTGGGACTTGGATTTTTCCTAATACGCCCGAATCTATTGTACTCGGCGCATGAATATAAATGATGGGTGGTTCAAATCAATAGGCTCTCCGATACCATCTTAGAATTTGAGTTAGGCCTAACTCAACCATTAAAACACTGGCTTGATAGGTGGGAAGTAAGAGCTACCCACCACTTGAATACATATTGTCATATCTTATCTCATCCAATATGGAACTCATAACAATTTGCATATCTGATACAAATCCATAAGTATGCTATTTACATGCCTAATATCTATTATCAAAACCATGTCTCTGACGTAATAATTATTTGGTTGTTTTGTGTTATGTTAGGTATGAATCACCTTACTGGAGATCACTTGCAGTAAACAGAATTCGGGTTGCATGGAGATACAGGAAGAAACGTCTAAGTCGAGCTAATACCACATATAATGATTATCAAACATTGATGCCATAG
Protein sequence

>MS.gene069664.t1

MQLRGRDVEQWMNHHCLPEELKRRVRQAERYSWAATRGVPEKMVLENLPEDLQTDIRRHLFKFVNKVRIFSHMAEDDPILDAIRERLIQTTYIKGSKFLSQGGLVQKMVFIVRGKLESVGEDGIPVPLSEGDACGEELLRWYLEQSSESKGTTILDIMRTCLKRSCRNHNMPQTMINRCGFSCRRYESPYWRSLAVNRIRVAWRYRKKRLSRANTTYNDYQTLMP