AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069727


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069727.t1 MTR_8g056940 90.960 177 16 0 1 177 43 219 2.27e-119 336
MS.gene069727.t1 MTR_4g124130 62.712 177 66 0 1 177 44 220 3.19e-79 235
MS.gene069727.t1 MTR_2g070060 61.017 177 69 0 1 177 43 219 7.65e-77 229
MS.gene069727.t1 MTR_2g070140 59.887 177 71 0 1 177 27 203 8.36e-77 228
MS.gene069727.t1 MTR_2g070070 62.712 177 66 0 1 177 43 219 1.74e-76 228
MS.gene069727.t1 MTR_3g467420 55.367 177 79 0 1 177 48 224 2.13e-73 220
MS.gene069727.t1 MTR_2g070200 61.714 175 65 2 2 174 44 218 3.13e-73 219
MS.gene069727.t1 MTR_2g070180 59.884 172 69 0 1 172 43 214 1.45e-72 218
MS.gene069727.t1 MTR_3g467430 55.932 177 78 0 1 177 48 224 1.56e-72 218
MS.gene069727.t1 MTR_2g070130 55.367 177 79 0 1 177 43 219 8.13e-72 216
MS.gene069727.t1 MTR_2g070150 62.500 160 60 0 1 160 43 202 8.94e-72 215
MS.gene069727.t1 MTR_2g070210 54.802 177 80 0 1 177 45 221 3.75e-70 212
MS.gene069727.t1 MTR_2g070110 58.757 177 72 1 1 177 43 218 1.96e-69 210
MS.gene069727.t1 MTR_2g070120 55.689 167 74 0 1 167 43 209 1.26e-64 197
MS.gene069727.t1 MTR_0186s0030 93.939 99 6 0 1 99 43 141 2.26e-63 192
MS.gene069727.t1 MTR_5g076900 52.326 172 81 1 1 172 46 216 3.25e-60 187
MS.gene069727.t1 MTR_7g065740 41.176 170 94 4 1 166 46 213 5.51e-34 120
MS.gene069727.t1 MTR_7g065720 38.235 170 99 4 1 166 46 213 4.10e-33 117
MS.gene069727.t1 MTR_7g065660 38.690 168 98 4 2 166 47 212 1.15e-32 116
MS.gene069727.t1 MTR_1g090060 38.012 171 101 3 1 167 42 211 1.41e-32 116
MS.gene069727.t1 MTR_7g065750 41.615 161 88 4 1 157 46 204 5.59e-32 114
MS.gene069727.t1 MTR_7g065270 39.326 178 100 5 1 175 46 218 1.23e-31 114
MS.gene069727.t1 MTR_4g059730 39.412 170 98 3 2 167 44 212 1.86e-31 113
MS.gene069727.t1 MTR_4g019780 35.714 168 103 4 2 166 48 213 8.30e-31 111
MS.gene069727.t1 MTR_7g065630 44.828 145 75 5 2 145 65 205 1.28e-30 111
MS.gene069727.t1 MTR_8g087425 36.478 159 98 2 1 157 44 201 1.88e-30 110
MS.gene069727.t1 MTR_7g065265 40.223 179 97 6 1 175 46 218 3.10e-30 110
MS.gene069727.t1 MTR_8g061950 40.625 160 88 4 1 153 46 205 5.18e-30 110
MS.gene069727.t1 MTR_4g019790 37.500 160 95 4 1 157 46 203 5.79e-30 109
MS.gene069727.t1 MTR_7g065230 40.113 177 93 7 2 175 47 213 1.22e-29 108
MS.gene069727.t1 MTR_6g080440 37.017 181 104 5 1 174 42 219 1.37e-29 108
MS.gene069727.t1 MTR_3g099757 47.244 127 66 1 1 127 44 169 2.27e-29 108
MS.gene069727.t1 MTR_5g037380 39.444 180 94 6 1 168 44 220 2.61e-29 108
MS.gene069727.t1 MTR_8g087410 38.621 145 86 2 1 143 45 188 4.20e-29 107
MS.gene069727.t1 MTR_7g065710 49.138 116 56 3 2 116 44 157 4.36e-29 107
MS.gene069727.t1 MTR_1g090100 38.824 170 99 3 1 166 41 209 9.09e-29 106
MS.gene069727.t1 MTR_7g065290 37.079 178 104 5 1 175 46 218 1.13e-28 106
MS.gene069727.t1 MTR_7g065590 40.268 149 82 5 1 145 46 191 2.00e-27 103
MS.gene069727.t1 MTR_7g065600 40.136 147 83 4 2 145 47 191 2.03e-27 103
MS.gene069727.t1 MTR_5g040430 31.902 163 104 2 2 157 47 209 2.28e-27 102
MS.gene069727.t1 MTR_1g090150 47.458 118 56 4 1 115 42 156 4.87e-27 102
MS.gene069727.t1 MTR_1g090070 37.143 175 96 5 2 167 49 218 5.55e-27 102
MS.gene069727.t1 MTR_2g072120 61.111 72 28 0 5 76 1 72 1.41e-26 96.7
MS.gene069727.t1 MTR_1g115195 36.747 166 95 4 1 159 42 204 3.60e-26 99.8
MS.gene069727.t1 MTR_7g065680 48.276 116 57 3 2 116 47 160 8.99e-26 98.6
MS.gene069727.t1 MTR_7g065700 50.000 114 54 3 2 114 47 158 2.73e-25 97.4
MS.gene069727.t1 MTR_1g090090 36.095 169 103 3 2 166 43 210 1.68e-24 95.5
MS.gene069727.t1 MTR_7g065260 38.095 147 86 4 2 145 54 198 6.56e-24 94.0
MS.gene069727.t1 MTR_7g065640 44.898 98 52 2 1 97 46 142 5.56e-23 91.3
MS.gene069727.t1 MTR_4g043640 86.047 43 6 0 106 148 44 86 2.26e-22 86.3
MS.gene069727.t1 MTR_7g100320 30.588 170 100 8 10 174 76 232 5.46e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069727.t1 AT1G78380 60.920 174 68 0 1 174 43 216 2.66e-74 222
MS.gene069727.t1 AT1G17180 58.757 177 73 0 1 177 43 219 1.27e-73 221
MS.gene069727.t1 AT1G78370 57.803 173 73 0 1 173 43 215 2.03e-73 220
MS.gene069727.t1 AT1G78340 59.884 172 69 0 1 172 43 214 2.68e-72 217
MS.gene069727.t1 AT1G17170 58.192 177 73 1 1 177 43 218 3.84e-72 217
MS.gene069727.t1 AT1G78360 57.558 172 73 0 1 172 44 215 2.87e-70 212
MS.gene069727.t1 AT1G53680 54.023 174 78 2 1 172 46 219 3.03e-66 202
MS.gene069727.t1 AT1G17190 52.874 174 82 0 1 174 44 217 4.04e-65 199
MS.gene069727.t1 AT1G78320 54.651 172 78 0 1 172 43 214 1.50e-63 195
MS.gene069727.t1 AT3G43800 51.744 172 80 2 1 169 45 216 2.72e-56 177
MS.gene069727.t1 AT1G78320 44.186 172 69 1 1 172 43 187 5.94e-44 144
MS.gene069727.t1 AT3G09270 46.939 147 76 1 1 145 46 192 2.89e-36 125
MS.gene069727.t1 AT2G29420 40.331 181 94 6 1 175 48 220 1.68e-35 124
MS.gene069727.t1 AT2G29470 36.872 179 107 4 1 175 47 223 1.01e-32 117
MS.gene069727.t1 AT1G10370 38.286 175 100 4 1 167 44 218 6.00e-32 114
MS.gene069727.t1 AT2G29460 39.326 178 102 4 1 174 46 221 1.63e-31 113
MS.gene069727.t1 AT1G59700 39.130 184 102 5 1 175 46 228 7.75e-31 112
MS.gene069727.t1 AT2G29480 38.509 161 93 4 1 157 46 204 1.50e-28 106
MS.gene069727.t1 AT2G29490 38.125 160 95 2 1 157 46 204 1.65e-28 105
MS.gene069727.t1 AT5G62480 37.143 140 80 1 2 133 48 187 7.74e-28 104
MS.gene069727.t1 AT1G10360 36.571 175 103 4 1 167 44 218 1.16e-27 103
MS.gene069727.t1 AT1G59670 36.612 183 108 4 1 175 46 228 1.43e-27 103
MS.gene069727.t1 AT1G74590 32.044 181 111 4 2 173 47 224 2.49e-27 103
MS.gene069727.t1 AT2G29450 47.154 123 58 4 1 118 45 165 2.83e-27 102
MS.gene069727.t1 AT1G27130 34.483 174 107 3 1 167 47 220 3.09e-27 102
MS.gene069727.t1 AT1G27140 34.615 182 109 4 1 175 48 226 3.47e-27 102
MS.gene069727.t1 AT2G29440 44.628 121 63 2 1 118 45 164 4.15e-26 99.8
MS.gene069727.t1 AT1G69930 32.370 173 110 3 2 167 52 224 1.32e-25 98.6
MS.gene069727.t1 AT1G69920 33.523 176 106 4 1 167 75 248 2.18e-23 93.2

Find 32 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAAACTCAAGCAAGATTT+TGG 0.133634 8.4:+53817951 MS.gene069727:CDS
GGAGTTGATTGAAGGCTTAA+AGG 0.261757 8.4:+53818347 MS.gene069727:CDS
ATAAAGATGGACTCAGAAAT+TGG 0.267987 8.4:-53817866 None:intergenic
CTTGGAGACAAGCCTTATTT+TGG 0.284960 8.4:+53818384 MS.gene069727:CDS
GTGATGTTGGAGGAAGGATA+TGG 0.346197 8.4:+53818289 MS.gene069727:CDS
AATTCCAGTTCTTATCCATA+AGG 0.411200 8.4:+53817838 MS.gene069727:CDS
TGATGAGGTTTGGAAGGATA+AGG 0.411738 8.4:+53817901 MS.gene069727:CDS
TTTCCCTTATGGATAAGAAC+TGG 0.413273 8.4:-53817842 None:intergenic
AAAGAGAGATGAGATTGAAT+TGG 0.418834 8.4:+53818317 MS.gene069727:CDS
TTGAGTATATTGATGAGGTT+TGG 0.428745 8.4:+53817891 MS.gene069727:CDS
TAAGGATCTGAGGGTAGAAA+TGG 0.431150 8.4:-53817926 None:intergenic
AATGTGTCACCACCAAAATA+AGG 0.433956 8.4:-53818396 None:intergenic
TGAGATTGAATTGGCAAAGA+AGG 0.443100 8.4:+53818326 MS.gene069727:CDS
ATTCCAGTTCTTATCCATAA+GGG 0.455725 8.4:+53817839 MS.gene069727:CDS
ATGAGTTTGTTGTTAACTAT+AGG 0.457851 8.4:+53818586 MS.gene069727:CDS
TATAAAACCAACTATGAAAA+GGG 0.461833 8.4:-53818443 None:intergenic
GTATAAAACCAACTATGAAA+AGG 0.463905 8.4:-53818444 None:intergenic
AAAATGCAGGTTGGTGATGT+TGG 0.476506 8.4:+53818276 MS.gene069727:intron
GCAAAGAAGGAGTTGATTGA+AGG 0.480756 8.4:+53818339 MS.gene069727:CDS
TGATGTTGGAGGAAGGATAT+GGG 0.485671 8.4:+53818290 MS.gene069727:CDS
AATATACTCAACAATAAAGA+TGG 0.489706 8.4:-53817879 None:intergenic
AAGGAATTAGAGAATGTGCT+TGG 0.497037 8.4:+53818366 MS.gene069727:CDS
CTATAGGAAGAGGTTAGAGT+TGG 0.508025 8.4:+53818602 MS.gene069727:CDS
TTGTTGTTAACTATAGGAAG+AGG 0.543349 8.4:+53818592 MS.gene069727:CDS
GTATATTGATGAGGTTTGGA+AGG 0.548215 8.4:+53817895 MS.gene069727:CDS
GTTGGAGGAAGGATATGGGC+TGG 0.551186 8.4:+53818294 MS.gene069727:CDS
ATTATGCGACTTCAAAGTTG+AGG 0.582566 8.4:+53818476 MS.gene069727:CDS
TATTGTTGAGTATATTGATG+AGG 0.592193 8.4:+53817886 MS.gene069727:CDS
ATGCAGGTTGGTGATGTTGG+AGG 0.604533 8.4:+53818279 MS.gene069727:intron
AGTGTGATAAACTGATTGCA+TGG 0.628334 8.4:+53818502 MS.gene069727:CDS
AGGTTGGTGATGTTGGAGGA+AGG 0.655636 8.4:+53818283 MS.gene069727:intron
GTGTGATAAACTGATTGCAT+GGG 0.669961 8.4:+53818503 MS.gene069727:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATATTTTTATTTATTTAC+TGG - chr8.4:53818062-53818081 None:intergenic 10.0%
!!! ATTTTTATTTATTTACTGGT+AGG - chr8.4:53818058-53818077 None:intergenic 15.0%
!! AATATACTCAACAATAAAGA+TGG - chr8.4:53817882-53817901 None:intergenic 20.0%
!! CATTCTTGATATTTAATCAA+TGG + chr8.4:53818168-53818187 MS.gene069727:intron 20.0%
!! TAATGTGTTAAAAAAAATGC+AGG + chr8.4:53818263-53818282 MS.gene069727:intron 20.0%
!! TATAAAACCAACTATGAAAA+GGG - chr8.4:53818446-53818465 None:intergenic 20.0%
!! TTCTCTTGTCTTTAATAATA+TGG - chr8.4:53818222-53818241 None:intergenic 20.0%
! AAACTCATATCTTATTAGGA+AGG - chr8.4:53818142-53818161 None:intergenic 25.0%
! GTATAAAACCAACTATGAAA+AGG - chr8.4:53818447-53818466 None:intergenic 25.0%
! TATTGTTGAGTATATTGATG+AGG + chr8.4:53817886-53817905 MS.gene069727:CDS 25.0%
! TCCTAAACTCATATCTTATT+AGG - chr8.4:53818146-53818165 None:intergenic 25.0%
! TCCTAATAAGATATGAGTTT+AGG + chr8.4:53818142-53818161 MS.gene069727:intron 25.0%
!!! ATGAGTTTGTTGTTAACTAT+AGG + chr8.4:53818586-53818605 MS.gene069727:CDS 25.0%
AAAGAGAGATGAGATTGAAT+TGG + chr8.4:53818317-53818336 MS.gene069727:CDS 30.0%
AATTCCAGTTCTTATCCATA+AGG + chr8.4:53817838-53817857 MS.gene069727:CDS 30.0%
ATTCCAGTTCTTATCCATAA+GGG + chr8.4:53817839-53817858 MS.gene069727:CDS 30.0%
CATATCTTATTAGGAAGGAA+AGG - chr8.4:53818137-53818156 None:intergenic 30.0%
GAATGAGACACAAAAAACTT+TGG - chr8.4:53818015-53818034 None:intergenic 30.0%
GTGTTAAAAAAAATGCAGGT+TGG + chr8.4:53818267-53818286 MS.gene069727:intron 30.0%
TTGAGTATATTGATGAGGTT+TGG + chr8.4:53817891-53817910 MS.gene069727:CDS 30.0%
! AAAACTCAAGCAAGATTTTG+GGG + chr8.4:53817953-53817972 MS.gene069727:CDS 30.0%
! ACTGGAATTTTCTTGTGAAT+TGG - chr8.4:53817827-53817846 None:intergenic 30.0%
! AGAAAACTCAAGCAAGATTT+TGG + chr8.4:53817951-53817970 MS.gene069727:CDS 30.0%
! ATAAAGATGGACTCAGAAAT+TGG - chr8.4:53817869-53817888 None:intergenic 30.0%
! GAAAACTCAAGCAAGATTTT+GGG + chr8.4:53817952-53817971 MS.gene069727:CDS 30.0%
! TTGTTGTTAACTATAGGAAG+AGG + chr8.4:53818592-53818611 MS.gene069727:CDS 30.0%
!!! ACAAGAAGGTAAGTTTTGTT+AGG + chr8.4:53817987-53818006 MS.gene069727:intron 30.0%
!!! GTTTTTTGTGTCTCATTCAA+TGG + chr8.4:53818017-53818036 MS.gene069727:intron 30.0%
AAGGAATTAGAGAATGTGCT+TGG + chr8.4:53818366-53818385 MS.gene069727:CDS 35.0%
AATGTGTCACCACCAAAATA+AGG - chr8.4:53818399-53818418 None:intergenic 35.0%
AGTGTGATAAACTGATTGCA+TGG + chr8.4:53818502-53818521 MS.gene069727:CDS 35.0%
ATTATGCGACTTCAAAGTTG+AGG + chr8.4:53818476-53818495 MS.gene069727:CDS 35.0%
GTATATTGATGAGGTTTGGA+AGG + chr8.4:53817895-53817914 MS.gene069727:CDS 35.0%
GTGTGATAAACTGATTGCAT+GGG + chr8.4:53818503-53818522 MS.gene069727:CDS 35.0%
TGAGATTGAATTGGCAAAGA+AGG + chr8.4:53818326-53818345 MS.gene069727:CDS 35.0%
TTTCCCTTATGGATAAGAAC+TGG - chr8.4:53817845-53817864 None:intergenic 35.0%
! AGTTTTCTCATAAGGATCTG+AGG - chr8.4:53817939-53817958 None:intergenic 35.0%
! CTTGCTTGAGTTTTCTCATA+AGG - chr8.4:53817947-53817966 None:intergenic 35.0%
! CTTTGATCCCTTTTCATAGT+TGG + chr8.4:53818436-53818455 MS.gene069727:CDS 35.0%
! GTTTTCTCATAAGGATCTGA+GGG - chr8.4:53817938-53817957 None:intergenic 35.0%
!!! ATTTTGGTGGTGACACATTT+GGG + chr8.4:53818400-53818419 MS.gene069727:CDS 35.0%
!!! CAGAAATTGGTTTTCCCTTA+TGG - chr8.4:53817856-53817875 None:intergenic 35.0%
!!! TATTTTGGTGGTGACACATT+TGG + chr8.4:53818399-53818418 MS.gene069727:CDS 35.0%
CTATAGGAAGAGGTTAGAGT+TGG + chr8.4:53818602-53818621 MS.gene069727:CDS 40.0%
TAAGGATCTGAGGGTAGAAA+TGG - chr8.4:53817929-53817948 None:intergenic 40.0%
TGATGAGGTTTGGAAGGATA+AGG + chr8.4:53817901-53817920 MS.gene069727:CDS 40.0%
TGATGTTGGAGGAAGGATAT+GGG + chr8.4:53818290-53818309 MS.gene069727:CDS 40.0%
! GCAAAGAAGGAGTTGATTGA+AGG + chr8.4:53818339-53818358 MS.gene069727:CDS 40.0%
! GGAGTTGATTGAAGGCTTAA+AGG + chr8.4:53818347-53818366 MS.gene069727:CDS 40.0%
! GGGTGATTTTGTCAACAAGA+AGG + chr8.4:53817973-53817992 MS.gene069727:CDS 40.0%
!! AAAATGCAGGTTGGTGATGT+TGG + chr8.4:53818276-53818295 MS.gene069727:intron 40.0%
!!! CTTGGAGACAAGCCTTATTT+TGG + chr8.4:53818384-53818403 MS.gene069727:CDS 40.0%
GTGATGTTGGAGGAAGGATA+TGG + chr8.4:53818289-53818308 MS.gene069727:CDS 45.0%
!!! GGAGACAAGCCTTATTTTGG+TGG + chr8.4:53818387-53818406 MS.gene069727:CDS 45.0%
!! AGGTTGGTGATGTTGGAGGA+AGG + chr8.4:53818283-53818302 MS.gene069727:intron 50.0%
!! ATGCAGGTTGGTGATGTTGG+AGG + chr8.4:53818279-53818298 MS.gene069727:intron 50.0%
GTTGGAGGAAGGATATGGGC+TGG + chr8.4:53818294-53818313 MS.gene069727:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 53817806 53818629 53817806 ID=MS.gene069727
chr8.4 mRNA 53817806 53818629 53817806 ID=MS.gene069727.t1;Parent=MS.gene069727
chr8.4 exon 53817806 53817994 53817806 ID=MS.gene069727.t1.exon1;Parent=MS.gene069727.t1
chr8.4 CDS 53817806 53817994 53817806 ID=cds.MS.gene069727.t1;Parent=MS.gene069727.t1
chr8.4 exon 53818285 53818629 53818285 ID=MS.gene069727.t1.exon2;Parent=MS.gene069727.t1
chr8.4 CDS 53818285 53818629 53818285 ID=cds.MS.gene069727.t1;Parent=MS.gene069727.t1
Gene Sequence

>MS.gene069727

ATGCTTTTGCAAATGAATCCAATTCACAAGAAAATTCCAGTTCTTATCCATAAGGGAAAACCAATTTCTGAGTCCATCTTTATTGTTGAGTATATTGATGAGGTTTGGAAGGATAAGGTTCCATTTCTACCCTCAGATCCTTATGAGAAAACTCAAGCAAGATTTTGGGGTGATTTTGTCAACAAGAAGGTAAGTTTTGTTAGGTACCAAAGTTTTTTGTGTCTCATTCAATGGCTTAACATATTTTTACCTACCAGTAAATAAATAAAAATATCTAATTTCTTACTTGTTAGCAGTAACTTTTGATATTAAGTAATAATACTTGTATCCTTTCCTTCCTAATAAGATATGAGTTTAGGAAACATTCTTGATATTTAATCAATGGATAGATTTATCATTCATCTTATTGTTATCCATATTATTAAAGACAAGAGAATATGCTAATTTTTATTCATTTTAATGTGTTAAAAAAAATGCAGGTTGGTGATGTTGGAGGAAGGATATGGGCTGGAAAGAGAGATGAGATTGAATTGGCAAAGAAGGAGTTGATTGAAGGCTTAAAGGAATTAGAGAATGTGCTTGGAGACAAGCCTTATTTTGGTGGTGACACATTTGGGTTTGTTGACATTGCTTTGATCCCTTTTCATAGTTGGTTTTATACTTATGAGAAATTATGCGACTTCAAAGTTGAGGAAGAGTGTGATAAACTGATTGCATGGGCAAAGAGATGCAAGCAAAGAGATAGTGTTTCTAAATCTGTTGCTGATGAGAAAGAAGTGTATGAGTTTGTTGTTAACTATAGGAAGAGGTTAGAGTTGGACTGA

Protein sequence

>MS.gene069727.t1

MLLQMNPIHKKIPVLIHKGKPISESIFIVEYIDEVWKDKVPFLPSDPYEKTQARFWGDFVNKKVGDVGGRIWAGKRDEIELAKKELIEGLKELENVLGDKPYFGGDTFGFVDIALIPFHSWFYTYEKLCDFKVEEECDKLIAWAKRCKQRDSVSKSVADEKEVYEFVVNYRKRLELD