Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070405.t1 | XP_024640815.1 | 62.6 | 334 | 67 | 8 | 1 | 290 | 1 | 320 | 2.90E-49 | 206.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070405.t1 | G7K4I5 | 58.2 | 359 | 69 | 9 | 1 | 290 | 1 | 347 | 3.5e-44 | 188.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene070405 | 0.805337 | 1.40E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene070405 | 0.812335 | 4.47E-51 | -1.69E-46 |
| MS.gene051789 | MS.gene070405 | 0.81495 | 1.19E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene070405 | 0.802622 | 5.14E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene070405 | 0.805291 | 1.43E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene070405 | -0.829831 | 4.13E-55 | -1.69E-46 |
| MS.gene058168 | MS.gene070405 | 0.804426 | 2.17E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene070405 | 0.803675 | 3.11E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene070405 | 0.829441 | 5.14E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070405.t1 | MTR_5g094950 | 57.103 | 359 | 73 | 8 | 1 | 290 | 1 | 347 | 1.22e-105 | 322 |
| MS.gene070405.t1 | MTR_5g094970 | 51.104 | 317 | 69 | 8 | 70 | 316 | 6 | 306 | 1.97e-75 | 237 |
| MS.gene070405.t1 | MTR_5g095050 | 36.945 | 563 | 160 | 17 | 1 | 372 | 1 | 559 | 1.34e-64 | 215 |
| MS.gene070405.t1 | MTR_5g094960 | 40.854 | 328 | 107 | 9 | 1 | 290 | 1 | 279 | 4.87e-52 | 180 |
| MS.gene070405.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 1.33e-49 | 174 |
| MS.gene070405.t1 | MTR_5g094850 | 40.058 | 342 | 132 | 9 | 3 | 309 | 1 | 304 | 2.26e-49 | 174 |
| MS.gene070405.t1 | MTR_5g094920 | 34.596 | 396 | 107 | 9 | 1 | 290 | 1 | 350 | 1.55e-38 | 145 |
| MS.gene070405.t1 | MTR_5g094990 | 40.705 | 312 | 124 | 13 | 1 | 290 | 1 | 273 | 1.52e-36 | 138 |
| MS.gene070405.t1 | MTR_5g095370 | 41.206 | 199 | 70 | 5 | 1 | 196 | 1 | 155 | 8.24e-32 | 120 |
| MS.gene070405.t1 | MTR_5g094910 | 34.731 | 334 | 104 | 11 | 1 | 301 | 1 | 253 | 3.04e-28 | 114 |
| MS.gene070405.t1 | MTR_5g095400 | 46.341 | 164 | 56 | 3 | 26 | 185 | 1 | 136 | 1.10e-27 | 114 |
| MS.gene070405.t1 | MTR_5g095430 | 45.806 | 155 | 52 | 3 | 35 | 185 | 1 | 127 | 9.89e-27 | 111 |
| MS.gene070405.t1 | MTR_5g077500 | 45.122 | 164 | 59 | 4 | 178 | 311 | 97 | 259 | 1.67e-24 | 102 |
| MS.gene070405.t1 | MTR_5g095390 | 44.086 | 186 | 67 | 5 | 30 | 202 | 20 | 181 | 2.64e-21 | 94.4 |
| MS.gene070405.t1 | MTR_5g095310 | 39.456 | 147 | 61 | 3 | 35 | 179 | 1 | 121 | 1.66e-19 | 89.4 |
| MS.gene070405.t1 | MTR_5g095360 | 41.121 | 107 | 52 | 3 | 61 | 158 | 1 | 105 | 1.61e-14 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 45 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGACCCATGTTCGATTT+TGG | 0.133002 | 5.3:-75861381 | None:intergenic |
| CTCAAAACCATTACATGTTA+AGG | 0.367230 | 5.3:+75862396 | MS.gene070405:CDS |
| TGAGTTAGATAATGAAGTTT+TGG | 0.377487 | 5.3:+75861940 | MS.gene070405:CDS |
| GTCATTTCTTCTAAGTTATG+AGG | 0.381921 | 5.3:-75861452 | None:intergenic |
| CTTACAACACAACGTTGAAA+TGG | 0.390778 | 5.3:+75861586 | MS.gene070405:CDS |
| TTGACATCCTTAACATGTAA+TGG | 0.392334 | 5.3:-75862403 | None:intergenic |
| GGGCTGCTTGCATCTTTGCC+TGG | 0.393057 | 5.3:-75862487 | None:intergenic |
| GAGAGGAACTTTGTCAGAAA+GGG | 0.410933 | 5.3:+75861651 | MS.gene070405:CDS |
| TTAGCTGATAGAGTCACTAT+TGG | 0.415973 | 5.3:+75861809 | MS.gene070405:CDS |
| TGAGCTAGAAGCTAAAGTTT+TGG | 0.417553 | 5.3:+75862027 | MS.gene070405:CDS |
| CCAATCTTCGGGTCACTCTC+TGG | 0.419534 | 5.3:-75862557 | None:intergenic |
| AAGAAAGAAGTACGTTGAGT+TGG | 0.438659 | 5.3:+75861838 | MS.gene070405:CDS |
| GGAGGATGTTGAAGCAAGTC+AGG | 0.455376 | 5.3:+75862231 | MS.gene070405:CDS |
| CGAGCTTCGAAGTAAATTGA+AGG | 0.461338 | 5.3:+75862099 | MS.gene070405:CDS |
| GACACATGAGGTGTTGCAAC+CGG | 0.466770 | 5.3:-75862274 | None:intergenic |
| AGAGAGGAACTTTGTCAGAA+AGG | 0.484616 | 5.3:+75861650 | MS.gene070405:CDS |
| ATGTCAAGAAAGCCAAAGTC+AGG | 0.490710 | 5.3:+75862419 | MS.gene070405:CDS |
| GAAGATGGTGATGAGGTTGA+TGG | 0.497731 | 5.3:+75862520 | MS.gene070405:CDS |
| TCTAACACTAGACCTGACTT+TGG | 0.506997 | 5.3:-75862431 | None:intergenic |
| AGCAAGAATCACGTAGTTGG+TGG | 0.527719 | 5.3:-75861405 | None:intergenic |
| AACAAGATGATTGATGGTCC+AGG | 0.535173 | 5.3:+75862469 | MS.gene070405:CDS |
| GAGGTTGATGGTATTGATGA+TGG | 0.540674 | 5.3:+75862532 | MS.gene070405:CDS |
| CTTATTCGAGTCTTTGAACA+CGG | 0.544179 | 5.3:+75862174 | MS.gene070405:CDS |
| TTCAGCAAGAATCACGTAGT+TGG | 0.558811 | 5.3:-75861408 | None:intergenic |
| GCATGAAAGAAATGCAAGGT+TGG | 0.577609 | 5.3:+75862210 | MS.gene070405:CDS |
| TGAGTTGAGGAAACTGTTTG+AGG | 0.587379 | 5.3:+75861769 | MS.gene070405:CDS |
| TGAAGTTGTAACAGATATGA+TGG | 0.587982 | 5.3:+75861427 | MS.gene070405:CDS |
| TCACCATCTTCAATCACAAG+TGG | 0.589505 | 5.3:-75862508 | None:intergenic |
| AGCCCACTTGTGATTGAAGA+TGG | 0.597478 | 5.3:+75862505 | MS.gene070405:CDS |
| GAAAGAGTTTCAGAAAAGAG+AGG | 0.598100 | 5.3:+75861634 | MS.gene070405:CDS |
| TACTATAAATGAGAAGCGTA+AGG | 0.599833 | 5.3:+75862144 | MS.gene070405:CDS |
| ATTGAACCCAAAATCGAACA+TGG | 0.604289 | 5.3:+75861374 | MS.gene070405:CDS |
| GTACGTTGAGTTGGAGTGTG+AGG | 0.619740 | 5.3:+75861847 | MS.gene070405:CDS |
| TGATCATGTTGCAAATGCAC+CGG | 0.620646 | 5.3:+75862255 | MS.gene070405:CDS |
| GCAACACCTCATGTGTCTGA+TGG | 0.630963 | 5.3:+75862280 | MS.gene070405:CDS |
| GAGAGAGGAACATTGAGTTG+AGG | 0.631931 | 5.3:+75861756 | MS.gene070405:CDS |
| TGAAAGAAATGCAAGGTTGG+AGG | 0.632876 | 5.3:+75862213 | MS.gene070405:CDS |
| TCTTTGAACACGGAGTTCAG+AGG | 0.634013 | 5.3:+75862184 | MS.gene070405:CDS |
| TTGAAATGGAGAAACTTGCA+AGG | 0.634595 | 5.3:+75861600 | MS.gene070405:CDS |
| CACCATCTTCAATCACAAGT+GGG | 0.636322 | 5.3:-75862507 | None:intergenic |
| CTGTTGTAGTACTAAGAGAG+AGG | 0.656292 | 5.3:+75861741 | MS.gene070405:CDS |
| TGTGATTGAAGATGGTGATG+AGG | 0.677350 | 5.3:+75862513 | MS.gene070405:CDS |
| GATTGCATGAAAGAAATGCA+AGG | 0.680697 | 5.3:+75862206 | MS.gene070405:CDS |
| TTGAACCCAAAATCGAACAT+GGG | 0.683973 | 5.3:+75861375 | MS.gene070405:CDS |
| TTTGTACCATCAGACACATG+AGG | 0.707522 | 5.3:-75862286 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTGTTTAAGTTATATTTTAC+AGG | + | chr5.3:75862360-75862379 | MS.gene070405:intron | 15.0% |
| ! | TTGAAAAACAAGATGATTGA+TGG | + | chr5.3:75862463-75862482 | MS.gene070405:CDS | 25.0% |
| !! | GAAGTACTAAGAAAAAAGAA+TGG | + | chr5.3:75861917-75861936 | MS.gene070405:CDS | 25.0% |
| !! | TGAGTTAGATAATGAAGTTT+TGG | + | chr5.3:75861940-75861959 | MS.gene070405:CDS | 25.0% |
| CTCAAAACCATTACATGTTA+AGG | + | chr5.3:75862396-75862415 | MS.gene070405:CDS | 30.0% | |
| GAAGTTGAAAGAAAAATGTG+TGG | + | chr5.3:75861874-75861893 | MS.gene070405:CDS | 30.0% | |
| GTCATTTCTTCTAAGTTATG+AGG | - | chr5.3:75861455-75861474 | None:intergenic | 30.0% | |
| TGAAGTTGTAACAGATATGA+TGG | + | chr5.3:75861427-75861446 | MS.gene070405:CDS | 30.0% | |
| TTGACATCCTTAACATGTAA+TGG | - | chr5.3:75862406-75862425 | None:intergenic | 30.0% | |
| ! | TACTATAAATGAGAAGCGTA+AGG | + | chr5.3:75862144-75862163 | MS.gene070405:CDS | 30.0% |
| ! | TGGAGATTTTGAATGAGAAA+TGG | + | chr5.3:75862053-75862072 | MS.gene070405:CDS | 30.0% |
| ! | TTTGGAGTTGAAGCAAAAAA+GGG | + | chr5.3:75861958-75861977 | MS.gene070405:CDS | 30.0% |
| !!! | TTTTGGAGTTGAAGCAAAAA+AGG | + | chr5.3:75861957-75861976 | MS.gene070405:CDS | 30.0% |
| AAAAATGTGTGGAGGATGAT+AGG | + | chr5.3:75861885-75861904 | MS.gene070405:CDS | 35.0% | |
| ATTGAACCCAAAATCGAACA+TGG | + | chr5.3:75861374-75861393 | MS.gene070405:CDS | 35.0% | |
| CTTACAACACAACGTTGAAA+TGG | + | chr5.3:75861586-75861605 | MS.gene070405:CDS | 35.0% | |
| CTTATTCGAGTCTTTGAACA+CGG | + | chr5.3:75862174-75862193 | MS.gene070405:CDS | 35.0% | |
| GAAAGAGTTTCAGAAAAGAG+AGG | + | chr5.3:75861634-75861653 | MS.gene070405:CDS | 35.0% | |
| GATTGCATGAAAGAAATGCA+AGG | + | chr5.3:75862206-75862225 | MS.gene070405:CDS | 35.0% | |
| GTTGAAAGAAAAATGTGTGG+AGG | + | chr5.3:75861877-75861896 | MS.gene070405:CDS | 35.0% | |
| TTAGCTGATAGAGTCACTAT+TGG | + | chr5.3:75861809-75861828 | MS.gene070405:CDS | 35.0% | |
| TTGAAATGGAGAAACTTGCA+AGG | + | chr5.3:75861600-75861619 | MS.gene070405:CDS | 35.0% | |
| TTGAACCCAAAATCGAACAT+GGG | + | chr5.3:75861375-75861394 | MS.gene070405:CDS | 35.0% | |
| ! | AAGAAAGAAGTACGTTGAGT+TGG | + | chr5.3:75861838-75861857 | MS.gene070405:CDS | 35.0% |
| ! | TGAGCTAGAAGCTAAAGTTT+TGG | + | chr5.3:75862027-75862046 | MS.gene070405:CDS | 35.0% |
| !!! | AGAAGCTAAAGTTTTGGAGT+TGG | + | chr5.3:75862033-75862052 | MS.gene070405:CDS | 35.0% |
| AGAGAGGAACTTTGTCAGAA+AGG | + | chr5.3:75861650-75861669 | MS.gene070405:CDS | 40.0% | |
| ATGTCAAGAAAGCCAAAGTC+AGG | + | chr5.3:75862419-75862438 | MS.gene070405:CDS | 40.0% | |
| CACCATCTTCAATCACAAGT+GGG | - | chr5.3:75862510-75862529 | None:intergenic | 40.0% | |
| CGAGCTTCGAAGTAAATTGA+AGG | + | chr5.3:75862099-75862118 | MS.gene070405:CDS | 40.0% | |
| CTGTTGTAGTACTAAGAGAG+AGG | + | chr5.3:75861741-75861760 | MS.gene070405:CDS | 40.0% | |
| GAGAGGAACTTTGTCAGAAA+GGG | + | chr5.3:75861651-75861670 | MS.gene070405:CDS | 40.0% | |
| GCATGAAAGAAATGCAAGGT+TGG | + | chr5.3:75862210-75862229 | MS.gene070405:CDS | 40.0% | |
| TCACCATCTTCAATCACAAG+TGG | - | chr5.3:75862511-75862530 | None:intergenic | 40.0% | |
| TCTAACACTAGACCTGACTT+TGG | - | chr5.3:75862434-75862453 | None:intergenic | 40.0% | |
| TGAAAGAAATGCAAGGTTGG+AGG | + | chr5.3:75862213-75862232 | MS.gene070405:CDS | 40.0% | |
| TGATCATGTTGCAAATGCAC+CGG | + | chr5.3:75862255-75862274 | MS.gene070405:CDS | 40.0% | |
| TTCAGCAAGAATCACGTAGT+TGG | - | chr5.3:75861411-75861430 | None:intergenic | 40.0% | |
| TTTGTACCATCAGACACATG+AGG | - | chr5.3:75862289-75862308 | None:intergenic | 40.0% | |
| ! | AACAAGATGATTGATGGTCC+AGG | + | chr5.3:75862469-75862488 | MS.gene070405:CDS | 40.0% |
| ! | AGATGACCCATGTTCGATTT+TGG | - | chr5.3:75861384-75861403 | None:intergenic | 40.0% |
| ! | GATGACCCATGTTCGATTTT+GGG | - | chr5.3:75861383-75861402 | None:intergenic | 40.0% |
| ! | TGAGTTGAGGAAACTGTTTG+AGG | + | chr5.3:75861769-75861788 | MS.gene070405:CDS | 40.0% |
| ! | TGTGATTGAAGATGGTGATG+AGG | + | chr5.3:75862513-75862532 | MS.gene070405:CDS | 40.0% |
| !! | GAGGTTGATGGTATTGATGA+TGG | + | chr5.3:75862532-75862551 | MS.gene070405:CDS | 40.0% |
| AAGCAAAAAAGGGTGGAGGA+TGG | + | chr5.3:75861968-75861987 | MS.gene070405:CDS | 45.0% | |
| AGCAAAAAAGGGTGGAGGAT+GGG | + | chr5.3:75861969-75861988 | MS.gene070405:CDS | 45.0% | |
| AGCAAGAATCACGTAGTTGG+TGG | - | chr5.3:75861408-75861427 | None:intergenic | 45.0% | |
| AGCCCACTTGTGATTGAAGA+TGG | + | chr5.3:75862505-75862524 | MS.gene070405:CDS | 45.0% | |
| GGAGTTGAAGCAAAAAAGGG+TGG | + | chr5.3:75861961-75861980 | MS.gene070405:CDS | 45.0% | |
| GTTGAAGCAAAAAAGGGTGG+AGG | + | chr5.3:75861964-75861983 | MS.gene070405:CDS | 45.0% | |
| TCTTTGAACACGGAGTTCAG+AGG | + | chr5.3:75862184-75862203 | MS.gene070405:CDS | 45.0% | |
| ! | GAAGATGGTGATGAGGTTGA+TGG | + | chr5.3:75862520-75862539 | MS.gene070405:CDS | 45.0% |
| ! | GAGAGAGGAACATTGAGTTG+AGG | + | chr5.3:75861756-75861775 | MS.gene070405:CDS | 45.0% |
| GCAACACCTCATGTGTCTGA+TGG | + | chr5.3:75862280-75862299 | MS.gene070405:CDS | 50.0% | |
| GGAGGATGTTGAAGCAAGTC+AGG | + | chr5.3:75862231-75862250 | MS.gene070405:CDS | 50.0% | |
| !! | GACACATGAGGTGTTGCAAC+CGG | - | chr5.3:75862277-75862296 | None:intergenic | 50.0% |
| !! | GTACGTTGAGTTGGAGTGTG+AGG | + | chr5.3:75861847-75861866 | MS.gene070405:CDS | 50.0% |
| GGGCTGCTTGCATCTTTGCC+TGG | - | chr5.3:75862490-75862509 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75861368 | 75862567 | 75861368 | ID=MS.gene070405 |
| chr5.3 | mRNA | 75861368 | 75862567 | 75861368 | ID=MS.gene070405.t1;Parent=MS.gene070405 |
| chr5.3 | exon | 75861368 | 75862309 | 75861368 | ID=MS.gene070405.t1.exon1;Parent=MS.gene070405.t1 |
| chr5.3 | CDS | 75861368 | 75862309 | 75861368 | ID=cds.MS.gene070405.t1;Parent=MS.gene070405.t1 |
| chr5.3 | exon | 75862382 | 75862567 | 75862382 | ID=MS.gene070405.t1.exon2;Parent=MS.gene070405.t1 |
| chr5.3 | CDS | 75862382 | 75862567 | 75862382 | ID=cds.MS.gene070405.t1;Parent=MS.gene070405.t1 |
| Gene Sequence |
| Protein sequence |
