AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070562

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070562.t1 MTR_5g040360 96.716 609 20 0 1 609 1 609 0.0 1240
MS.gene070562.t1 MTR_8g055840 84.754 610 92 1 1 609 1 610 0.0 1109
MS.gene070562.t1 MTR_3g040370 72.727 594 158 3 15 608 17 606 0.0 942
MS.gene070562.t1 MTR_4g098890 66.121 611 190 6 8 609 9 611 0.0 874
MS.gene070562.t1 MTR_1g101370 56.020 598 244 8 18 600 25 618 0.0 697
MS.gene070562.t1 MTR_8g100075 52.572 622 264 7 9 608 17 629 0.0 691
MS.gene070562.t1 MTR_0019s0120 54.918 610 251 8 18 608 31 635 0.0 690
MS.gene070562.t1 MTR_7g076170 53.560 618 256 8 11 601 14 627 0.0 690
MS.gene070562.t1 MTR_8g432680 52.131 610 263 9 17 601 27 632 0.0 664
MS.gene070562.t1 MTR_7g006060 50.755 530 248 4 77 593 36 565 0.0 566
MS.gene070562.t1 MTR_0019s0120 52.849 509 216 8 18 507 31 534 0.0 542
MS.gene070562.t1 MTR_8g071280 45.849 530 276 7 84 606 70 595 3.44e-165 484
MS.gene070562.t1 MTR_5g026930 42.010 607 321 10 2 599 6 590 2.34e-162 477
MS.gene070562.t1 MTR_7g076150 44.096 542 274 8 71 599 62 587 1.04e-160 473
MS.gene070562.t1 MTR_8g432620 43.503 531 271 8 82 599 86 600 7.84e-160 471
MS.gene070562.t1 MTR_5g080720 43.738 535 275 11 75 596 116 637 6.88e-153 454
MS.gene070562.t1 MTR_1g075900 40.312 640 335 16 1 609 1 624 5.61e-146 436
MS.gene070562.t1 MTR_1g442840 40.230 609 309 14 1 575 1 588 1.18e-145 436
MS.gene070562.t1 MTR_1g069785 39.360 625 317 17 1 601 1 587 2.31e-145 434
MS.gene070562.t1 MTR_3g068990 43.902 533 265 11 82 597 151 666 1.62e-143 431
MS.gene070562.t1 MTR_1g442840 45.094 479 236 9 111 575 10 475 7.80e-142 422
MS.gene070562.t1 MTR_1g029730 43.396 530 273 13 84 598 141 658 5.57e-141 424
MS.gene070562.t1 MTR_7g076150 44.444 459 226 8 154 599 2 444 1.36e-136 406
MS.gene070562.t1 MTR_5g080720 43.789 475 241 11 75 536 116 577 8.07e-134 403
MS.gene070562.t1 MTR_5g026930 41.553 515 271 9 2 507 6 499 3.43e-132 397
MS.gene070562.t1 MTR_5g083150 39.504 524 276 14 92 599 100 598 2.30e-131 398
MS.gene070562.t1 MTR_5g021100 41.120 518 269 15 93 590 329 830 6.98e-126 391
MS.gene070562.t1 MTR_3g096480 40.684 526 276 13 93 599 268 776 7.72e-125 387
MS.gene070562.t1 MTR_3g071530 38.657 551 292 17 62 599 129 646 8.96e-125 382
MS.gene070562.t1 MTR_8g076650 41.426 519 265 15 93 590 387 887 1.01e-124 389
MS.gene070562.t1 MTR_1g051055 39.326 534 284 13 87 599 89 603 3.11e-124 380
MS.gene070562.t1 MTR_8g093880 40.494 526 277 13 93 599 298 806 3.18e-122 381
MS.gene070562.t1 MTR_3g088790 40.454 529 274 16 93 599 233 742 1.34e-120 375
MS.gene070562.t1 MTR_3g074780 30.944 572 340 17 70 604 140 693 1.24e-89 292
MS.gene070562.t1 MTR_4g083030 31.216 551 338 12 70 598 72 603 1.63e-89 289
MS.gene070562.t1 MTR_1g022255 33.146 534 306 15 84 599 199 699 7.07e-87 285
MS.gene070562.t1 MTR_5g064080 29.595 642 371 21 2 599 66 670 2.54e-85 280
MS.gene070562.t1 MTR_1g022255 28.500 400 241 12 84 466 199 570 2.41e-42 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070562.t1 AT1G26850 76.911 615 132 3 3 609 4 616 0.0 1016
MS.gene070562.t1 AT1G26850 76.911 615 132 3 3 609 4 616 0.0 1016
MS.gene070562.t1 AT4G18030 66.889 601 185 5 9 601 11 605 0.0 860
MS.gene070562.t1 AT1G26850 75.400 500 115 2 3 494 4 503 0.0 812
MS.gene070562.t1 AT4G10440 54.221 616 248 6 9 601 14 618 0.0 704
MS.gene070562.t1 AT1G33170 51.238 646 266 7 1 604 1 639 0.0 687
MS.gene070562.t1 AT4G00750 51.471 612 282 5 5 601 13 624 0.0 670
MS.gene070562.t1 AT2G45750 51.356 627 276 9 5 607 7 628 0.0 661
MS.gene070562.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 558
MS.gene070562.t1 AT2G43200 52.023 519 238 5 84 594 91 606 0.0 558
MS.gene070562.t1 AT1G31850 45.216 533 277 8 84 606 76 603 1.25e-168 493
MS.gene070562.t1 AT1G31850 45.216 533 277 8 84 606 76 603 1.25e-168 493
MS.gene070562.t1 AT1G31850 45.216 533 277 8 84 606 76 603 1.25e-168 493
MS.gene070562.t1 AT1G31850 45.216 533 277 8 84 606 76 603 1.25e-168 493
MS.gene070562.t1 AT4G19120 43.761 553 295 9 69 609 52 600 2.86e-167 490
MS.gene070562.t1 AT4G19120 43.761 553 295 9 69 609 52 600 2.86e-167 490
MS.gene070562.t1 AT4G00740 45.113 532 263 9 87 605 84 599 3.11e-162 477
MS.gene070562.t1 AT4G00740 45.113 532 263 9 87 605 84 599 3.11e-162 477
MS.gene070562.t1 AT5G06050 42.614 528 277 11 84 598 149 663 2.99e-145 436
MS.gene070562.t1 AT1G04430 40.747 589 313 12 8 576 9 581 1.05e-143 431
MS.gene070562.t1 AT1G04430 40.747 589 313 12 8 576 9 581 1.05e-143 431
MS.gene070562.t1 AT1G04430 40.747 589 313 12 8 576 9 581 1.05e-143 431
MS.gene070562.t1 AT2G39750 42.056 535 284 11 78 597 167 690 3.16e-143 431
MS.gene070562.t1 AT3G23300 42.804 542 275 13 81 601 76 603 5.48e-142 426
MS.gene070562.t1 AT3G23300 42.804 542 275 13 81 601 76 603 5.48e-142 426
MS.gene070562.t1 AT4G14360 43.860 513 257 11 81 575 73 572 1.59e-141 424
MS.gene070562.t1 AT4G14360 43.860 513 257 11 81 575 73 572 1.59e-141 424
MS.gene070562.t1 AT1G77260 41.869 535 283 12 76 597 135 654 2.01e-138 418
MS.gene070562.t1 AT4G19120 44.789 451 234 8 69 507 52 499 1.28e-136 408
MS.gene070562.t1 AT2G40280 40.720 528 269 13 87 599 83 581 3.94e-134 405
MS.gene070562.t1 AT2G34300 41.917 532 274 13 93 606 250 764 1.25e-133 409
MS.gene070562.t1 AT2G34300 41.917 532 274 13 93 606 250 764 1.25e-133 409
MS.gene070562.t1 AT2G34300 41.917 532 274 13 93 606 250 764 1.25e-133 409
MS.gene070562.t1 AT1G29470 41.985 524 271 13 93 599 250 757 1.68e-132 406
MS.gene070562.t1 AT1G29470 41.985 524 271 13 93 599 250 757 1.68e-132 406
MS.gene070562.t1 AT5G04060 39.773 528 287 9 87 599 86 597 9.86e-132 399
MS.gene070562.t1 AT5G14430 39.024 615 322 16 11 599 17 604 1.43e-131 399
MS.gene070562.t1 AT5G14430 38.699 615 324 16 11 599 17 604 4.36e-130 395
MS.gene070562.t1 AT3G56080 40.115 521 272 12 93 599 114 608 2.08e-128 390
MS.gene070562.t1 AT3G10200 39.024 533 286 12 87 599 75 588 1.02e-126 385
MS.gene070562.t1 AT5G64030 42.178 505 262 12 93 579 308 800 2.30e-126 392
MS.gene070562.t1 AT3G51070 41.045 536 272 19 92 604 380 894 2.64e-125 391
MS.gene070562.t1 AT3G10200 40.373 483 250 11 87 550 75 538 2.40e-118 364
MS.gene070562.t1 AT3G56080 41.556 450 231 11 93 528 114 545 1.84e-113 350
MS.gene070562.t1 AT3G51070 42.576 458 227 17 92 528 380 822 1.97e-107 342
MS.gene070562.t1 AT1G13860 32.523 535 310 13 79 584 76 588 8.57e-92 295
MS.gene070562.t1 AT1G13860 32.523 535 310 13 79 584 76 588 8.57e-92 295
MS.gene070562.t1 AT1G13860 32.523 535 310 13 79 584 76 588 8.57e-92 295
MS.gene070562.t1 AT1G13860 32.523 535 310 13 79 584 76 588 8.57e-92 295
MS.gene070562.t1 AT2G03480 30.757 634 353 17 3 598 7 592 1.03e-88 287
MS.gene070562.t1 AT2G03480 30.573 628 373 15 3 598 7 603 3.83e-88 286
MS.gene070562.t1 AT1G19430 33.333 522 303 14 93 599 231 722 8.24e-87 285
MS.gene070562.t1 AT1G19430 33.333 522 303 14 93 599 231 722 8.24e-87 285
MS.gene070562.t1 AT1G78240 31.560 564 329 17 74 598 133 678 1.66e-85 281
MS.gene070562.t1 AT1G78240 31.560 564 329 17 74 598 133 678 1.66e-85 281
MS.gene070562.t1 AT1G13860 34.773 440 250 11 171 584 4 432 9.68e-84 270
MS.gene070562.t1 AT2G03480 30.000 410 249 9 3 393 7 397 1.42e-47 172
MS.gene070562.t1 AT1G19430 29.231 390 237 11 93 468 231 595 1.77e-44 167

Find 172 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCGGTTATTGGGTGCTTTC+GGG 0.168627 5.3:-28298716 MS.gene070562:CDS
TGAGAATCAAAGCTTAAATT+TGG 0.224891 5.3:+28299834 None:intergenic
CTGACTGTTCCATTCTTTAT+CGG 0.229941 5.3:+28299411 None:intergenic
CCAGTTGGGGTGCATATCTT+TGG 0.240419 5.3:-28299051 MS.gene070562:CDS
TTCTAAGACCTGGCGGTTAT+TGG 0.250522 5.3:-28298727 MS.gene070562:CDS
TGGCGGTTATTGGGTGCTTT+CGG 0.251157 5.3:-28298717 MS.gene070562:CDS
TTCTCGTTGCTTAATTCCTT+GGG 0.266033 5.3:-28298876 MS.gene070562:CDS
TGTTGTTTCTTCTACATATT+AGG 0.267388 5.3:-28299939 MS.gene070562:CDS
GTTCTACTACCAGAAGAAAT+TGG 0.274137 5.3:+28299999 None:intergenic
TGTCAGCCCCTTGAGGGAAT+TGG 0.275822 5.3:+28299463 None:intergenic
TCCATCTGGAGCCTTTGATA+TGG 0.309219 5.3:-28298906 MS.gene070562:CDS
TCATTGCAGGTATAAGAAAA+TGG 0.309228 5.3:-28298297 MS.gene070562:intron
TGAACACATTTCCCTCATAT+TGG 0.312865 5.3:+28299505 None:intergenic
GTTCTCGTTGCTTAATTCCT+TGG 0.315484 5.3:-28298877 MS.gene070562:CDS
GACAGCAATTTCATTCTTCT+CGG 0.325154 5.3:+28298577 None:intergenic
GTTGCCAGTGGTTCTGTTCC+CGG 0.338306 5.3:-28298194 MS.gene070562:CDS
GTGCAGATCTTTATAGTAGT+TGG 0.347054 5.3:-28299966 MS.gene070562:CDS
GTCAGCCCCTTGAGGGAATT+GGG 0.348985 5.3:+28299464 None:intergenic
ATGGCTAAACCAATTTCTTC+TGG 0.354667 5.3:-28300008 MS.gene070562:CDS
CGGCTCTTTGGCCATGGAAA+TGG 0.358252 5.3:+28299597 None:intergenic
CTATTCAATCTCCTAAGTTA+TGG 0.359289 5.3:-28298028 MS.gene070562:CDS
TCATAGGCATTTATCATGAT+TGG 0.359736 5.3:-28297941 MS.gene070562:intron
TTGTAGGGTGCGTTTGCATA+TGG 0.367932 5.3:+28299564 None:intergenic
GGAACATAATCACGGCTCTT+TGG 0.368346 5.3:+28299585 None:intergenic
ATAAAATTGCCTTATCCATC+TGG 0.370154 5.3:-28298920 MS.gene070562:CDS
AATTGCTGTCAAGCAGTATT+GGG 0.374007 5.3:-28297047 MS.gene070562:CDS
CGAAGTAGACAAGAAGATTC+TGG 0.381386 5.3:-28298524 MS.gene070562:CDS
CGGAATATAACAGCACCTTC+AGG 0.382318 5.3:+28297178 None:intergenic
ACAGAACCACTGGCAACTTT+AGG 0.382460 5.3:+28298200 None:intergenic
GCGTGGCAGAGAACTGGTTT+TGG 0.384451 5.3:-28299915 MS.gene070562:CDS
TAACATAGAAGACATTCTTC+TGG 0.389124 5.3:-28297221 MS.gene070562:CDS
TCTAAGACCTGGCGGTTATT+GGG 0.391716 5.3:-28298726 MS.gene070562:CDS
AGGGAATTGGGTTCCACCAC+CGG 0.404220 5.3:+28299476 None:intergenic
TTCCACTGTAAGACTCTTGT+AGG 0.404847 5.3:+28299548 None:intergenic
AGGTTGCTAAGCTCCTTTGC+TGG 0.405835 5.3:-28298610 MS.gene070562:CDS
TATTCAATCTCCTAAGTTAT+GGG 0.408342 5.3:-28298027 MS.gene070562:CDS
ATAAATAAGCTCCTGGACTC+TGG 0.414706 5.3:-28298104 MS.gene070562:CDS
TCTTCCTCAAGATCCTCCTT+AGG 0.414914 5.3:+28298650 None:intergenic
ATCAAGGTAAAGAAAATTGT+TGG 0.416395 5.3:-28297136 MS.gene070562:CDS
TAATTAGCCTTCCAATGGAT+TGG 0.416920 5.3:+28298689 None:intergenic
CACTATGTCATTTGCACCTA+GGG 0.417543 5.3:-28299014 MS.gene070562:CDS
TAATTGCTGTCAAGCAGTAT+TGG 0.427048 5.3:-28297048 MS.gene070562:CDS
AGTACAATTTGCACTTGAAA+GGG 0.428802 5.3:-28298975 MS.gene070562:CDS
ATCCTCCTTAGGTCTTTGCC+AGG 0.433073 5.3:+28298661 None:intergenic
TCAGAACTGGATCCAATATG+AGG 0.434365 5.3:-28299517 MS.gene070562:CDS
CAATTGGAGGTTACTAAGAA+AGG 0.434885 5.3:-28299876 MS.gene070562:CDS
CAAGAGTCTTACAGTGGAAA+AGG 0.435530 5.3:-28299544 MS.gene070562:CDS
ATTTATCAGCGAGGACTCAT+AGG 0.437788 5.3:-28297957 MS.gene070562:CDS
CCAAAGATATGCACCCCAAC+TGG 0.442920 5.3:+28299051 None:intergenic
GCATATAGTCTCTGTGGAAA+AGG 0.443799 5.3:+28298230 None:intergenic
CACCTTCAGGTCGTAAAATC+CGG 0.448926 5.3:+28297191 None:intergenic
TTGCAAGGCTTAAATACCTT+AGG 0.449525 5.3:+28299768 None:intergenic
GGCACGCCGTTGATCATGGC+AGG 0.451114 5.3:+28299722 None:intergenic
CAACAGATGCTGATGATGTT+TGG 0.451762 5.3:-28298484 MS.gene070562:intron
GGCAGATGGGATTTATATGA+TGG 0.451926 5.3:-28298762 MS.gene070562:intron
GTATGTCTCAGCTGAAACTC+CGG 0.453655 5.3:+28298175 None:intergenic
GAGGTTACTAAGAAAGGTGC+TGG 0.454381 5.3:-28299870 MS.gene070562:CDS
TTAATTCCTTGGGGATCAAA+TGG 0.459096 5.3:-28298866 MS.gene070562:intron
TCTCCGTGGACTTTACGATT+AGG 0.459427 5.3:+28298260 None:intergenic
TTAGGTGCGTGGCAGAGAAC+TGG 0.459773 5.3:-28299921 MS.gene070562:CDS
CATGGATATGAATGCTGGCT+TGG 0.460213 5.3:-28298066 MS.gene070562:CDS
AAGTACAATTTGCACTTGAA+AGG 0.461765 5.3:-28298976 MS.gene070562:CDS
CCTGCCGTCATTGGTGTCCT+AGG 0.462243 5.3:-28298947 MS.gene070562:CDS
ACAGCACTGGACACTGGTTG+TGG 0.462399 5.3:-28299387 MS.gene070562:CDS
GGGAATTGGGTTCCACCACC+GGG 0.470402 5.3:+28299477 None:intergenic
AATGCGATGGGATACTAAAC+TGG 0.479392 5.3:-28297110 MS.gene070562:CDS
TATATTTGTCAGCCCCTTGA+GGG 0.481593 5.3:+28299457 None:intergenic
TGGAAAAGGCTATTCAGAAC+TGG 0.488249 5.3:-28299530 MS.gene070562:CDS
GGTTGCTAAGCTCCTTTGCT+GGG 0.492174 5.3:-28298609 MS.gene070562:CDS
CAAGAAAATAAATAAGCTCC+TGG 0.495511 5.3:-28298111 MS.gene070562:CDS
TGAATGAGGTCGTACGTCCT+TGG 0.496095 5.3:+28297491 None:intergenic
GTGTTCAGATTCCCCGGTGG+TGG 0.497584 5.3:-28299489 MS.gene070562:CDS
GTAACATACCCTTTCGGTGC+TGG 0.499813 5.3:+28299621 None:intergenic
TCCACTGTAAGACTCTTGTA+GGG 0.500127 5.3:+28299549 None:intergenic
GTATGTTTCTCAGCTATAGT+AGG 0.501052 5.3:+28297990 None:intergenic
TAATCACGGCTCTTTGGCCA+TGG 0.503657 5.3:+28299591 None:intergenic
TGGAAGGTATCGCAATATCA+TGG 0.504476 5.3:-28298084 MS.gene070562:CDS
TCATGGCACGCCGTTGATCA+TGG 0.504850 5.3:+28299718 None:intergenic
ACGACCTCATTCATAGCAAT+GGG 0.505409 5.3:-28297481 MS.gene070562:CDS
TGCGATACCTTCCAGAGTCC+AGG 0.507113 5.3:+28298093 None:intergenic
GGAAGGCTAATTACAAAGCC+TGG 0.510311 5.3:-28298679 MS.gene070562:CDS
ACATTCTTCTGGAGATGGAC+CGG 0.512221 5.3:-28297210 MS.gene070562:CDS
TGAAGTTGATATCTTAATCA+AGG 0.512501 5.3:-28297152 MS.gene070562:CDS
GTCGTACGTCCTTGGATATG+TGG 0.513166 5.3:+28297499 None:intergenic
TCAAAGGCTCCAGATGGATA+AGG 0.515562 5.3:+28298911 None:intergenic
CTTTGTAATTAGCCTTCCAA+TGG 0.517155 5.3:+28298684 None:intergenic
ATGGATATGAATGCTGGCTT+GGG 0.519668 5.3:-28298065 MS.gene070562:CDS
TACGACCTCATTCATAGCAA+TGG 0.523430 5.3:-28297482 MS.gene070562:CDS
TTCTCACCATTTGATCCCCA+AGG 0.523610 5.3:+28298860 None:intergenic
TGAGGTTGATTCAAAACCTA+AGG 0.526700 5.3:-28299784 MS.gene070562:CDS
AATGGAGTAACATACCCTTT+CGG 0.527057 5.3:+28299615 None:intergenic
CAGCACTGGACACTGGTTGT+GGG 0.532258 5.3:-28299386 MS.gene070562:CDS
CGGTCTTCTTCTCCCAGCAA+AGG 0.532439 5.3:+28298597 None:intergenic
AATATCATGGATATGAATGC+TGG 0.533826 5.3:-28298071 MS.gene070562:CDS
AGAAGAATGAAATTGCTGTC+TGG 0.538296 5.3:-28298574 MS.gene070562:CDS
GTTTGATACCAGCACCGAAA+GGG 0.539221 5.3:-28299629 MS.gene070562:CDS
AGGGGTGTACCTGCCGTCAT+TGG 0.542782 5.3:-28298956 MS.gene070562:CDS
CAAAGCCTGGCAAAGACCTA+AGG 0.543305 5.3:-28298666 MS.gene070562:CDS
TCTGTGACACCGATAAAGAA+TGG 0.545947 5.3:-28299420 MS.gene070562:CDS
TTTGCACAGGTTGCCAGTTG+GGG 0.547748 5.3:-28299064 MS.gene070562:intron
ACCAGGATGATAAAGAAAAG+TGG 0.553954 5.3:-28298154 MS.gene070562:CDS
GGAAATGTGTTCAGATTCCC+CGG 0.555028 5.3:-28299495 MS.gene070562:CDS
TTAGCCTTCCAATGGATTGG+AGG 0.556029 5.3:+28298692 None:intergenic
CGATAAAGAATGGAACAGTC+AGG 0.559758 5.3:-28299410 MS.gene070562:CDS
TCACTATGTCATTTGCACCT+AGG 0.560218 5.3:-28299015 MS.gene070562:CDS
TTTCGGGACCTCCAATCCAT+TGG 0.560894 5.3:-28298700 MS.gene070562:CDS
ACATTCATGACCCATAACTT+AGG 0.560995 5.3:+28298017 None:intergenic
TGTGCTTCATGTGAGTCCCT+AGG 0.562927 5.3:+28298998 None:intergenic
GAACCACTGGCAACTTTAGG+AGG 0.564388 5.3:+28298203 None:intergenic
TGGAACAGTCAGGACAGCAC+TGG 0.564499 5.3:-28299400 MS.gene070562:CDS
AGAACAAAGAAAGATTGAAG+AGG 0.565209 5.3:-28298630 MS.gene070562:CDS
AGAAGACATTCTTCTGGAGA+TGG 0.567755 5.3:-28297215 MS.gene070562:CDS
AAGGTAAAGAAAATTGTTGG+AGG 0.568236 5.3:-28297133 MS.gene070562:CDS
CAGAACTGGATCCAATATGA+GGG 0.569553 5.3:-28299516 MS.gene070562:CDS
TCGATATATCGAGCCTTGCA+AGG 0.569809 5.3:+28299753 None:intergenic
AACTCCGGGAACAGAACCAC+TGG 0.570803 5.3:+28298190 None:intergenic
GTCAGGACAGCACTGGACAC+TGG 0.572331 5.3:-28299393 MS.gene070562:CDS
GGGACCTCCAATCCATTGGA+AGG 0.573733 5.3:-28298696 MS.gene070562:CDS
TAAGGTATTTAAGCCTTGCA+AGG 0.576659 5.3:-28299766 MS.gene070562:CDS
TATGTCTCAGCTGAAACTCC+GGG 0.580149 5.3:+28298176 None:intergenic
ATAAGCTCCTGGACTCTGGA+AGG 0.581133 5.3:-28298100 MS.gene070562:CDS
AGAACAATGTGCCATATCAA+AGG 0.583862 5.3:+28298895 None:intergenic
GTGGAACCCAATTCCCTCAA+GGG 0.584099 5.3:-28299470 MS.gene070562:CDS
AACCGCCAGGTCTTAGAACT+CGG 0.584438 5.3:+28298732 None:intergenic
ACACGCCCTGCCATGATCAA+CGG 0.586626 5.3:-28299728 MS.gene070562:CDS
GAAAGCACCCAATAACCGCC+AGG 0.587918 5.3:+28298719 None:intergenic
GGTGGAACCCAATTCCCTCA+AGG 0.590459 5.3:-28299471 MS.gene070562:CDS
AAAATATGATTTATCGAGAG+AGG 0.590909 5.3:-28299683 MS.gene070562:CDS
GCCATATCAAAGGCTCCAGA+TGG 0.590985 5.3:+28298905 None:intergenic
TTAAGCTTTGATTCTCACCA+TGG 0.593567 5.3:-28299828 MS.gene070562:CDS
AAGACCTAAGGAGGATCTTG+AGG 0.593947 5.3:-28298654 MS.gene070562:CDS
AGCACTGGACACTGGTTGTG+GGG 0.594228 5.3:-28299385 MS.gene070562:intron
ATATATTTGTCAGCCCCTTG+AGG 0.597173 5.3:+28299456 None:intergenic
AATTGTTGGAGGAATGCGAT+GGG 0.598415 5.3:-28297122 MS.gene070562:CDS
GAACAAGTGGACCATCCTCA+TGG 0.600875 5.3:+28297083 None:intergenic
GGTATGTTACTCCATTTCCA+TGG 0.603095 5.3:-28299608 MS.gene070562:CDS
AGAAGTTAGCAAAATTGATG+AGG 0.606974 5.3:-28299802 MS.gene070562:CDS
TCTCGTTGCTTAATTCCTTG+GGG 0.606987 5.3:-28298875 MS.gene070562:CDS
TGTTTGATACCAGCACCGAA+AGG 0.609194 5.3:-28299630 MS.gene070562:CDS
AGTTTCAGCTGAGACATACC+AGG 0.611606 5.3:-28298171 MS.gene070562:CDS
GTTCCTCCTAAAGTTGCCAG+TGG 0.612508 5.3:-28298206 MS.gene070562:CDS
CCTAGGACACCAATGACGGC+AGG 0.614190 5.3:+28298947 None:intergenic
TTTACGATTAGGAGTAACAC+AGG 0.615416 5.3:+28298271 None:intergenic
AGCTTTGATTCTCACCATGG+TGG 0.615893 5.3:-28299825 MS.gene070562:CDS
TGGGCTCTTTAGTCTGTACA+AGG 0.617149 5.3:-28297462 MS.gene070562:CDS
AGAGCCCATTGCTATGAATG+AGG 0.631489 5.3:+28297477 None:intergenic
GCACGCCGTTGATCATGGCA+GGG 0.632559 5.3:+28299723 None:intergenic
GGTTCCTAGGACACCAATGA+CGG 0.632608 5.3:+28298943 None:intergenic
AAGCTTTCTCCACATATCCA+AGG 0.633136 5.3:-28297508 MS.gene070562:CDS
AGCCTGGCAAAGACCTAAGG+AGG 0.634638 5.3:-28298663 MS.gene070562:CDS
AAACTGGTCGACCATGAGGA+TGG 0.636228 5.3:-28297094 MS.gene070562:CDS
GTTGACCGAGTTCTAAGACC+TGG 0.636678 5.3:-28298737 MS.gene070562:CDS
GTACAATTTGCACTTGAAAG+GGG 0.638641 5.3:-28298974 MS.gene070562:CDS
TCTTCTACATATTAGGTGCG+TGG 0.640208 5.3:-28299932 MS.gene070562:CDS
TTGCATATGGAACATAATCA+CGG 0.641842 5.3:+28299577 None:intergenic
TGGAACCCAATTCCCTCAAG+GGG 0.646344 5.3:-28299469 MS.gene070562:CDS
TGTAACTTCTCTTCTTCACG+AGG 0.648313 5.3:+28299654 None:intergenic
GGAACTGCATATAGTCTCTG+TGG 0.648475 5.3:+28298224 None:intergenic
ACTCCTAATCGTAAAGTCCA+CGG 0.651226 5.3:-28298263 MS.gene070562:CDS
AAATTGTTGGAGGAATGCGA+TGG 0.656944 5.3:-28297123 MS.gene070562:CDS
TACTAAACTGGTCGACCATG+AGG 0.660050 5.3:-28297098 MS.gene070562:CDS
AGGTTACTAAGAAAGGTGCT+GGG 0.674354 5.3:-28299869 MS.gene070562:CDS
GACCGAGTTCTAAGACCTGG+CGG 0.681581 5.3:-28298734 MS.gene070562:CDS
GAGTGGTTATTGTGTTGACG+TGG 0.687579 5.3:+28297009 None:intergenic
CTCGGTGTCATTTATCAGCG+AGG 0.696680 5.3:-28297966 MS.gene070562:CDS
GTAACTTCTCTTCTTCACGA+GGG 0.701314 5.3:+28299655 None:intergenic
GAAAAGGCTTAAGATCTCCG+TGG 0.706045 5.3:+28298246 None:intergenic
ATAGCTGAGAAACATACACT+CGG 0.706522 5.3:-28297984 MS.gene070562:CDS
GGAATTGGGTTCCACCACCG+GGG 0.720073 5.3:+28299478 None:intergenic
ACCCTACAAGAGTCTTACAG+TGG 0.737297 5.3:-28299550 MS.gene070562:CDS
TTAAGATATCAACTTCATCG+CGG 0.764001 5.3:+28297158 None:intergenic
AATGTGTTCAGATTCCCCGG+TGG 0.776641 5.3:-28299492 MS.gene070562:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGAATAACCATATAATTAA+AGG + chr5.3:28299600-28299619 None:intergenic 15.0%
!! TGAGTATTCCTTTAATTATA+TGG - chr5.3:28299589-28299608 MS.gene070562:CDS 20.0%
!!! AATCATATTTTGTCTAGAAA+AGG + chr5.3:28297331-28297350 None:intergenic 20.0%
!!! CATAAAGGCATAAATAAATA+AGG + chr5.3:28299484-28299503 None:intergenic 20.0%
! AAAATATGATTTATCGAGAG+AGG - chr5.3:28297340-28297359 MS.gene070562:intron 25.0%
! AAAGTTGTGTACAAAAATCT+TGG - chr5.3:28298678-28298697 MS.gene070562:CDS 25.0%
! AATGAATGGAATATCAACAA+TGG + chr5.3:28298609-28298628 None:intergenic 25.0%
! ATCAAGGTAAAGAAAATTGT+TGG - chr5.3:28299887-28299906 MS.gene070562:CDS 25.0%
! GAATGCATTATAAGTTGTAT+AGG - chr5.3:28299649-28299668 MS.gene070562:CDS 25.0%
! GAGATAGAGATAAACATATA+CGG - chr5.3:28299310-28299329 MS.gene070562:intron 25.0%
! TATTCAATCTCCTAAGTTAT+GGG - chr5.3:28298996-28299015 MS.gene070562:CDS 25.0%
! TGAGAATCAAAGCTTAAATT+TGG + chr5.3:28297192-28297211 None:intergenic 25.0%
! TGTTAGTAGAACTAAATGAA+TGG + chr5.3:28298623-28298642 None:intergenic 25.0%
! TTATAATGCATTCTATGCAA+AGG + chr5.3:28299642-28299661 None:intergenic 25.0%
!!! TGAAGTTGATATCTTAATCA+AGG - chr5.3:28299871-28299890 MS.gene070562:CDS 25.0%
!!! TGTTGTTTCTTCTACATATT+AGG - chr5.3:28297084-28297103 MS.gene070562:CDS 25.0%
AAGGTAAAGAAAATTGTTGG+AGG - chr5.3:28299890-28299909 MS.gene070562:CDS 30.0%
AAGTACAATTTGCACTTGAA+AGG - chr5.3:28298047-28298066 MS.gene070562:CDS 30.0%
AATATCATGGATATGAATGC+TGG - chr5.3:28298952-28298971 MS.gene070562:CDS 30.0%
AGAACAAAGAAAGATTGAAG+AGG - chr5.3:28298393-28298412 MS.gene070562:intron 30.0%
AGAAGTTAGCAAAATTGATG+AGG - chr5.3:28297221-28297240 MS.gene070562:CDS 30.0%
AGTACAATTTGCACTTGAAA+GGG - chr5.3:28298048-28298067 MS.gene070562:CDS 30.0%
ATAAAATTGCCTTATCCATC+TGG - chr5.3:28298103-28298122 MS.gene070562:CDS 30.0%
ATGACAAAAGAATACGATAC+AGG + chr5.3:28297692-28297711 None:intergenic 30.0%
ATTAGCAGTATAGTTAGTTC+AGG - chr5.3:28297731-28297750 MS.gene070562:intron 30.0%
CAAGAAAATAAATAAGCTCC+TGG - chr5.3:28298912-28298931 MS.gene070562:CDS 30.0%
CTATTCAATCTCCTAAGTTA+TGG - chr5.3:28298995-28299014 MS.gene070562:CDS 30.0%
GACACTCTGAATAAAATAGA+AGG + chr5.3:28299127-28299146 None:intergenic 30.0%
TAACATAGAAGACATTCTTC+TGG - chr5.3:28299802-28299821 MS.gene070562:CDS 30.0%
TATTTATGCCTTTATGATCC+AGG - chr5.3:28299488-28299507 MS.gene070562:CDS 30.0%
TCATAGGCATTTATCATGAT+TGG - chr5.3:28299082-28299101 MS.gene070562:intron 30.0%
TCATTGCAGGTATAAGAAAA+TGG - chr5.3:28298726-28298745 MS.gene070562:CDS 30.0%
TGCATTATAAGTTGTATAGG+TGG - chr5.3:28299652-28299671 MS.gene070562:CDS 30.0%
TTAAGATATCAACTTCATCG+CGG + chr5.3:28299868-28299887 None:intergenic 30.0%
TTAGCAGTATAGTTAGTTCA+GGG - chr5.3:28297732-28297751 MS.gene070562:intron 30.0%
TTATCAGTGATAAAACCACT+CGG - chr5.3:28299700-28299719 MS.gene070562:CDS 30.0%
TTGCATATGGAACATAATCA+CGG + chr5.3:28297449-28297468 None:intergenic 30.0%
TTGTCATTGTGAATTCCTTA+GGG - chr5.3:28297666-28297685 MS.gene070562:intron 30.0%
TTTGTCATTGTGAATTCCTT+AGG - chr5.3:28297665-28297684 MS.gene070562:intron 30.0%
! AAAGGGAGATCTTTTACAAT+TGG - chr5.3:28297131-28297150 MS.gene070562:CDS 30.0%
! AAATAACGAAAGTGCATCTT+GGG - chr5.3:28298230-28298249 MS.gene070562:CDS 30.0%
! ACAGCAATTAGTATCTTTTC+AGG + chr5.3:28299965-28299984 None:intergenic 30.0%
! AGATGGATAAGGCAATTTTA+TGG + chr5.3:28298104-28298123 None:intergenic 30.0%
! ATTTTGTCTAGAAAAGGTCA+TGG + chr5.3:28297325-28297344 None:intergenic 30.0%
! TAAATAACGAAAGTGCATCT+TGG - chr5.3:28298229-28298248 MS.gene070562:CDS 30.0%
! TGCATTTGATGATTAGATAC+AGG + chr5.3:28299778-28299797 None:intergenic 30.0%
!! TACAAAGTGTTGTCAAATAG+AGG - chr5.3:28297832-28297851 MS.gene070562:intron 30.0%
AAAAGAGAACTAAAACCGAG+TGG + chr5.3:28299718-28299737 None:intergenic 35.0%
AAGGAAAAACATGCGAGAAA+AGG + chr5.3:28299465-28299484 None:intergenic 35.0%
AATGGAGTAACATACCCTTT+CGG + chr5.3:28297411-28297430 None:intergenic 35.0%
AATTGCTGTCAAGCAGTATT+GGG - chr5.3:28299976-28299995 MS.gene070562:CDS 35.0%
ACAAAAATCTTGGACTGAGT+TGG - chr5.3:28298688-28298707 MS.gene070562:CDS 35.0%
ACATTCATGACCCATAACTT+AGG + chr5.3:28299009-28299028 None:intergenic 35.0%
ACCAGGATGATAAAGAAAAG+TGG - chr5.3:28298869-28298888 MS.gene070562:CDS 35.0%
AGAACAATGTGCCATATCAA+AGG + chr5.3:28298131-28298150 None:intergenic 35.0%
AGAAGAATGAAATTGCTGTC+TGG - chr5.3:28298449-28298468 MS.gene070562:intron 35.0%
AGGAAAAACATGCGAGAAAA+GGG + chr5.3:28299464-28299483 None:intergenic 35.0%
ATAGCTGAGAAACATACACT+CGG - chr5.3:28299039-28299058 MS.gene070562:CDS 35.0%
ATGGCTAAACCAATTTCTTC+TGG - chr5.3:28297015-28297034 MS.gene070562:CDS 35.0%
CAATTGGAGGTTACTAAGAA+AGG - chr5.3:28297147-28297166 MS.gene070562:CDS 35.0%
CTAGGTATCTGTAATGTGTA+AGG - chr5.3:28297932-28297951 MS.gene070562:intron 35.0%
CTGACTGTTCCATTCTTTAT+CGG + chr5.3:28297615-28297634 None:intergenic 35.0%
GACAGCAATTTCATTCTTCT+CGG + chr5.3:28298449-28298468 None:intergenic 35.0%
GTACAATTTGCACTTGAAAG+GGG - chr5.3:28298049-28298068 MS.gene070562:CDS 35.0%
GTATGTTTCTCAGCTATAGT+AGG + chr5.3:28299036-28299055 None:intergenic 35.0%
GTGCAGATCTTTATAGTAGT+TGG - chr5.3:28297057-28297076 MS.gene070562:CDS 35.0%
GTTCTACTACCAGAAGAAAT+TGG + chr5.3:28297027-28297046 None:intergenic 35.0%
TAATTGCTGTCAAGCAGTAT+TGG - chr5.3:28299975-28299994 MS.gene070562:CDS 35.0%
TGAACACATTTCCCTCATAT+TGG + chr5.3:28297521-28297540 None:intergenic 35.0%
TTAATTCCTTGGGGATCAAA+TGG - chr5.3:28298157-28298176 MS.gene070562:CDS 35.0%
TTAGCACAACTTTCTGTGAT+CGG - chr5.3:28299432-28299451 MS.gene070562:CDS 35.0%
TTCTCGTTGCTTAATTCCTT+GGG - chr5.3:28298147-28298166 MS.gene070562:CDS 35.0%
TTGCAAGGCTTAAATACCTT+AGG + chr5.3:28297258-28297277 None:intergenic 35.0%
TTTACGATTAGGAGTAACAC+AGG + chr5.3:28298755-28298774 None:intergenic 35.0%
! AAGGCAATTTTATGGTTCCT+AGG + chr5.3:28298096-28298115 None:intergenic 35.0%
! CGTTTTCATGCAATACATCA+AGG + chr5.3:28299393-28299412 None:intergenic 35.0%
! TAAGGTATTTAAGCCTTGCA+AGG - chr5.3:28297257-28297276 MS.gene070562:intron 35.0%
! TGAGGTTGATTCAAAACCTA+AGG - chr5.3:28297239-28297258 MS.gene070562:intron 35.0%
! TTAAGCTTTGATTCTCACCA+TGG - chr5.3:28297195-28297214 MS.gene070562:CDS 35.0%
!! AACATATACGGTGTCACATA+CGG - chr5.3:28299322-28299341 MS.gene070562:intron 35.0%
!! AGTATCTTTTCAGGAACAAG+TGG + chr5.3:28299956-28299975 None:intergenic 35.0%
!! CTTTGTAATTAGCCTTCCAA+TGG + chr5.3:28298342-28298361 None:intergenic 35.0%
!! TAATTAGCCTTCCAATGGAT+TGG + chr5.3:28298337-28298356 None:intergenic 35.0%
!! TCCACTTTTCTTTATCATCC+TGG + chr5.3:28298873-28298892 None:intergenic 35.0%
!!! TGAGTCAGTTTGTTTTAGCT+AGG - chr5.3:28297914-28297933 MS.gene070562:intron 35.0%
!!! TGGATTTGTTTTGTCATTGC+AGG - chr5.3:28298713-28298732 MS.gene070562:CDS 35.0%
AAATTGTTGGAGGAATGCGA+TGG - chr5.3:28299900-28299919 MS.gene070562:CDS 40.0%
AAGCTTTCTCCACATATCCA+AGG - chr5.3:28299515-28299534 MS.gene070562:CDS 40.0%
AATGAACACAACTGACGAAG+GGG + chr5.3:28299742-28299761 None:intergenic 40.0%
AATGCGATGGGATACTAAAC+TGG - chr5.3:28299913-28299932 MS.gene070562:CDS 40.0%
AATTGTTGGAGGAATGCGAT+GGG - chr5.3:28299901-28299920 MS.gene070562:CDS 40.0%
ACGACCTCATTCATAGCAAT+GGG - chr5.3:28299542-28299561 MS.gene070562:CDS 40.0%
ACTCCTAATCGTAAAGTCCA+CGG - chr5.3:28298760-28298779 MS.gene070562:CDS 40.0%
AGAATGAACACAACTGACGA+AGG + chr5.3:28299744-28299763 None:intergenic 40.0%
ATAAATAAGCTCCTGGACTC+TGG - chr5.3:28298919-28298938 MS.gene070562:CDS 40.0%
ATATATTTGTCAGCCCCTTG+AGG + chr5.3:28297570-28297589 None:intergenic 40.0%
ATTTATCAGCGAGGACTCAT+AGG - chr5.3:28299066-28299085 MS.gene070562:intron 40.0%
CAAGAGTCTTACAGTGGAAA+AGG - chr5.3:28297479-28297498 MS.gene070562:CDS 40.0%
CAGAACTGGATCCAATATGA+GGG - chr5.3:28297507-28297526 MS.gene070562:CDS 40.0%
CGAAGTAGACAAGAAGATTC+TGG - chr5.3:28298499-28298518 MS.gene070562:CDS 40.0%
CGATAAAGAATGGAACAGTC+AGG - chr5.3:28297613-28297632 MS.gene070562:intron 40.0%
GAATGAACACAACTGACGAA+GGG + chr5.3:28299743-28299762 None:intergenic 40.0%
GCATATAGTCTCTGTGGAAA+AGG + chr5.3:28298796-28298815 None:intergenic 40.0%
GGCAGATGGGATTTATATGA+TGG - chr5.3:28298261-28298280 MS.gene070562:CDS 40.0%
GGGTTGTCAAATAGTAGCTA+TGG - chr5.3:28297752-28297771 MS.gene070562:intron 40.0%
GGTATGTTACTCCATTTCCA+TGG - chr5.3:28297415-28297434 MS.gene070562:intron 40.0%
GTAACTTCTCTTCTTCACGA+GGG + chr5.3:28297371-28297390 None:intergenic 40.0%
GTTCTCGTTGCTTAATTCCT+TGG - chr5.3:28298146-28298165 MS.gene070562:CDS 40.0%
TACGACCTCATTCATAGCAA+TGG - chr5.3:28299541-28299560 MS.gene070562:CDS 40.0%
TAGCACAACTTTCTGTGATC+GGG - chr5.3:28299433-28299452 MS.gene070562:CDS 40.0%
TATATTTGTCAGCCCCTTGA+GGG + chr5.3:28297569-28297588 None:intergenic 40.0%
TCAGAACTGGATCCAATATG+AGG - chr5.3:28297506-28297525 MS.gene070562:CDS 40.0%
TCCACTGTAAGACTCTTGTA+GGG + chr5.3:28297477-28297496 None:intergenic 40.0%
TCTCGTTGCTTAATTCCTTG+GGG - chr5.3:28298148-28298167 MS.gene070562:CDS 40.0%
TCTGTGACACCGATAAAGAA+TGG - chr5.3:28297603-28297622 MS.gene070562:intron 40.0%
TCTTCTACATATTAGGTGCG+TGG - chr5.3:28297091-28297110 MS.gene070562:CDS 40.0%
TGGAAAAGGCTATTCAGAAC+TGG - chr5.3:28297493-28297512 MS.gene070562:CDS 40.0%
TGTAACTTCTCTTCTTCACG+AGG + chr5.3:28297372-28297391 None:intergenic 40.0%
TTCCACTGTAAGACTCTTGT+AGG + chr5.3:28297478-28297497 None:intergenic 40.0%
! AATCTTGGACTGAGTTGGAA+TGG - chr5.3:28298693-28298712 MS.gene070562:CDS 40.0%
! AGAAGACATTCTTCTGGAGA+TGG - chr5.3:28299808-28299827 MS.gene070562:CDS 40.0%
! AGTGCATCTTGGGTTGTTAT+TGG - chr5.3:28298240-28298259 MS.gene070562:CDS 40.0%
! CAACAGATGCTGATGATGTT+TGG - chr5.3:28298539-28298558 MS.gene070562:CDS 40.0%
! CACTATGTCATTTGCACCTA+GGG - chr5.3:28298009-28298028 MS.gene070562:CDS 40.0%
! GGGAGATCTTTTACAATTGG+AGG - chr5.3:28297134-28297153 MS.gene070562:CDS 40.0%
! TCACTATGTCATTTGCACCT+AGG - chr5.3:28298008-28298027 MS.gene070562:CDS 40.0%
! TTGGGTTGTTATTGGCAGAT+GGG - chr5.3:28298248-28298267 MS.gene070562:CDS 40.0%
! TTTTGCTAACTTCTCCACCA+TGG + chr5.3:28297215-28297234 None:intergenic 40.0%
!! AGGTTACTAAGAAAGGTGCT+GGG - chr5.3:28297154-28297173 MS.gene070562:CDS 40.0%
!! ATGGATATGAATGCTGGCTT+GGG - chr5.3:28298958-28298977 MS.gene070562:CDS 40.0%
!! TGGAAGGTATCGCAATATCA+TGG - chr5.3:28298939-28298958 MS.gene070562:CDS 40.0%
!! TGTGTAAGGTTGTTTTGCAC+AGG - chr5.3:28297946-28297965 MS.gene070562:CDS 40.0%
!!! CAGAGAACTGGTTTTGGAAA+GGG - chr5.3:28297114-28297133 MS.gene070562:CDS 40.0%
AAATAGAGGCAGCACTGTAG+TGG - chr5.3:28297846-28297865 MS.gene070562:intron 45.0%
AAGACCTAAGGAGGATCTTG+AGG - chr5.3:28298369-28298388 MS.gene070562:intron 45.0%
ACAGAACCACTGGCAACTTT+AGG + chr5.3:28298826-28298845 None:intergenic 45.0%
ACCCTACAAGAGTCTTACAG+TGG - chr5.3:28297473-28297492 MS.gene070562:CDS 45.0%
AGAATACGATACAGGCCCTA+AGG + chr5.3:28297684-28297703 None:intergenic 45.0%
AGAGCCCATTGCTATGAATG+AGG + chr5.3:28299549-28299568 None:intergenic 45.0%
AGTTTCAGCTGAGACATACC+AGG - chr5.3:28298852-28298871 MS.gene070562:intron 45.0%
CACCTTCAGGTCGTAAAATC+CGG + chr5.3:28299835-28299854 None:intergenic 45.0%
CGGAATATAACAGCACCTTC+AGG + chr5.3:28299848-28299867 None:intergenic 45.0%
GAAAAGGCTTAAGATCTCCG+TGG + chr5.3:28298780-28298799 None:intergenic 45.0%
GCTTCACACCTGGATCATAA+AGG + chr5.3:28299499-28299518 None:intergenic 45.0%
GGAAATGTGTTCAGATTCCC+CGG - chr5.3:28297528-28297547 MS.gene070562:intron 45.0%
GGAACATAATCACGGCTCTT+TGG + chr5.3:28297441-28297460 None:intergenic 45.0%
GGAACTGCATATAGTCTCTG+TGG + chr5.3:28298802-28298821 None:intergenic 45.0%
GGAAGGCTAATTACAAAGCC+TGG - chr5.3:28298344-28298363 MS.gene070562:intron 45.0%
GTATGTCTCAGCTGAAACTC+CGG + chr5.3:28298851-28298870 None:intergenic 45.0%
TACTAAACTGGTCGACCATG+AGG - chr5.3:28299925-28299944 MS.gene070562:CDS 45.0%
TATGTCTCAGCTGAAACTCC+GGG + chr5.3:28298850-28298869 None:intergenic 45.0%
TCAAAGGCTCCAGATGGATA+AGG + chr5.3:28298115-28298134 None:intergenic 45.0%
TCGATATATCGAGCCTTGCA+AGG + chr5.3:28297273-28297292 None:intergenic 45.0%
TCTCCGTGGACTTTACGATT+AGG + chr5.3:28298766-28298785 None:intergenic 45.0%
TCTTCCTCAAGATCCTCCTT+AGG + chr5.3:28298376-28298395 None:intergenic 45.0%
TGACTCAGTGATGTTAGTCG+CGG + chr5.3:28297901-28297920 None:intergenic 45.0%
TGGGCTCTTTAGTCTGTACA+AGG - chr5.3:28299561-28299580 MS.gene070562:CDS 45.0%
TTCTCACCATTTGATCCCCA+AGG + chr5.3:28298166-28298185 None:intergenic 45.0%
TTGTAGGGTGCGTTTGCATA+TGG + chr5.3:28297462-28297481 None:intergenic 45.0%
! ACATTCTTCTGGAGATGGAC+CGG - chr5.3:28299813-28299832 MS.gene070562:CDS 45.0%
! AGCTTTGATTCTCACCATGG+TGG - chr5.3:28297198-28297217 MS.gene070562:CDS 45.0%
! CATGGATATGAATGCTGGCT+TGG - chr5.3:28298957-28298976 MS.gene070562:CDS 45.0%
! CTTGGGTTGTTATTGGCAGA+TGG - chr5.3:28298247-28298266 MS.gene070562:CDS 45.0%
! GAGGTTACTAAGAAAGGTGC+TGG - chr5.3:28297153-28297172 MS.gene070562:CDS 45.0%
! GTGATGTTAGTCGCGGATTA+TGG + chr5.3:28297894-28297913 None:intergenic 45.0%
! GTTTGATACCAGCACCGAAA+GGG - chr5.3:28297394-28297413 MS.gene070562:intron 45.0%
! TCCATCTGGAGCCTTTGATA+TGG - chr5.3:28298117-28298136 MS.gene070562:CDS 45.0%
! TCTAAGACCTGGCGGTTATT+GGG - chr5.3:28298297-28298316 MS.gene070562:intron 45.0%
! TGTTTGATACCAGCACCGAA+AGG - chr5.3:28297393-28297412 MS.gene070562:intron 45.0%
! TTTTGCACAGGTTGCCAGTT+GGG - chr5.3:28297958-28297977 MS.gene070562:CDS 45.0%
!! TTAGCCTTCCAATGGATTGG+AGG + chr5.3:28298334-28298353 None:intergenic 45.0%
!! TTCTAAGACCTGGCGGTTAT+TGG - chr5.3:28298296-28298315 MS.gene070562:intron 45.0%
!!! GCAGAGAACTGGTTTTGGAA+AGG - chr5.3:28297113-28297132 MS.gene070562:CDS 45.0%
AAACTGGTCGACCATGAGGA+TGG - chr5.3:28299929-28299948 MS.gene070562:CDS 50.0%
AACCGCCAGGTCTTAGAACT+CGG + chr5.3:28298294-28298313 None:intergenic 50.0%
AATGTGTTCAGATTCCCCGG+TGG - chr5.3:28297531-28297550 MS.gene070562:intron 50.0%
AGGTTGCTAAGCTCCTTTGC+TGG - chr5.3:28298413-28298432 MS.gene070562:intron 50.0%
ATAAGCTCCTGGACTCTGGA+AGG - chr5.3:28298923-28298942 MS.gene070562:CDS 50.0%
ATCCTCCTTAGGTCTTTGCC+AGG + chr5.3:28298365-28298384 None:intergenic 50.0%
CAAAGCCTGGCAAAGACCTA+AGG - chr5.3:28298357-28298376 MS.gene070562:intron 50.0%
CCAAAGATATGCACCCCAAC+TGG + chr5.3:28297975-28297994 None:intergenic 50.0%
CCAGTTGGGGTGCATATCTT+TGG - chr5.3:28297972-28297991 MS.gene070562:CDS 50.0%
GAACAAGTGGACCATCCTCA+TGG + chr5.3:28299943-28299962 None:intergenic 50.0%
GAACCACTGGCAACTTTAGG+AGG + chr5.3:28298823-28298842 None:intergenic 50.0%
GCCATATCAAAGGCTCCAGA+TGG + chr5.3:28298121-28298140 None:intergenic 50.0%
GGTTCCTAGGACACCAATGA+CGG + chr5.3:28298083-28298102 None:intergenic 50.0%
GGTTGCTAAGCTCCTTTGCT+GGG - chr5.3:28298414-28298433 MS.gene070562:intron 50.0%
GTCGTACGTCCTTGGATATG+TGG + chr5.3:28299527-28299546 None:intergenic 50.0%
GTGGAACCCAATTCCCTCAA+GGG - chr5.3:28297553-28297572 MS.gene070562:intron 50.0%
GTTCCTCCTAAAGTTGCCAG+TGG - chr5.3:28298817-28298836 MS.gene070562:intron 50.0%
TAATCACGGCTCTTTGGCCA+TGG + chr5.3:28297435-28297454 None:intergenic 50.0%
TGAATGAGGTCGTACGTCCT+TGG + chr5.3:28299535-28299554 None:intergenic 50.0%
TGGAACCCAATTCCCTCAAG+GGG - chr5.3:28297554-28297573 MS.gene070562:intron 50.0%
TGGCGGTTATTGGGTGCTTT+CGG - chr5.3:28298306-28298325 MS.gene070562:intron 50.0%
TGTGCTTCATGTGAGTCCCT+AGG + chr5.3:28298028-28298047 None:intergenic 50.0%
TGTGGAGAAAGCTTCACACC+TGG + chr5.3:28299509-28299528 None:intergenic 50.0%
TTTCGGGACCTCCAATCCAT+TGG - chr5.3:28298323-28298342 MS.gene070562:intron 50.0%
TTTGCACAGGTTGCCAGTTG+GGG - chr5.3:28297959-28297978 MS.gene070562:CDS 50.0%
! GACCGGATTTTACGACCTGA+AGG - chr5.3:28299830-28299849 MS.gene070562:CDS 50.0%
! GTAACATACCCTTTCGGTGC+TGG + chr5.3:28297405-28297424 None:intergenic 50.0%
! GTTTTGCACAGGTTGCCAGT+TGG - chr5.3:28297957-28297976 MS.gene070562:CDS 50.0%
!! CTCGGTGTCATTTATCAGCG+AGG - chr5.3:28299057-28299076 MS.gene070562:CDS 50.0%
!! GTTGACCGAGTTCTAAGACC+TGG - chr5.3:28298286-28298305 MS.gene070562:CDS 50.0%
AACTCCGGGAACAGAACCAC+TGG + chr5.3:28298836-28298855 None:intergenic 55.0%
ACACGCCCTGCCATGATCAA+CGG - chr5.3:28297295-28297314 MS.gene070562:intron 55.0%
AGCCTGGCAAAGACCTAAGG+AGG - chr5.3:28298360-28298379 MS.gene070562:intron 55.0%
AGGGAATTGGGTTCCACCAC+CGG + chr5.3:28297550-28297569 None:intergenic 55.0%
CGGCTCTTTGGCCATGGAAA+TGG + chr5.3:28297429-28297448 None:intergenic 55.0%
CGGTCTTCTTCTCCCAGCAA+AGG + chr5.3:28298429-28298448 None:intergenic 55.0%
GGCGGTTATTGGGTGCTTTC+GGG - chr5.3:28298307-28298326 MS.gene070562:intron 55.0%
GGGACCTCCAATCCATTGGA+AGG - chr5.3:28298327-28298346 MS.gene070562:intron 55.0%
GGTGGAACCCAATTCCCTCA+AGG - chr5.3:28297552-28297571 MS.gene070562:intron 55.0%
GTCAGCCCCTTGAGGGAATT+GGG + chr5.3:28297562-28297581 None:intergenic 55.0%
TCATGGCACGCCGTTGATCA+TGG + chr5.3:28297308-28297327 None:intergenic 55.0%
TGCGATACCTTCCAGAGTCC+AGG + chr5.3:28298933-28298952 None:intergenic 55.0%
TGGAACAGTCAGGACAGCAC+TGG - chr5.3:28297623-28297642 MS.gene070562:intron 55.0%
TGTCAGCCCCTTGAGGGAAT+TGG + chr5.3:28297563-28297582 None:intergenic 55.0%
TTAGGTGCGTGGCAGAGAAC+TGG - chr5.3:28297102-28297121 MS.gene070562:CDS 55.0%
! ACAGCACTGGACACTGGTTG+TGG - chr5.3:28297636-28297655 MS.gene070562:intron 55.0%
! AGCACTGGACACTGGTTGTG+GGG - chr5.3:28297638-28297657 MS.gene070562:intron 55.0%
! CAGCACTGGACACTGGTTGT+GGG - chr5.3:28297637-28297656 MS.gene070562:intron 55.0%
! GTTGCCAGTGGTTCTGTTCC+CGG - chr5.3:28298829-28298848 MS.gene070562:intron 55.0%
!! GAAAGCACCCAATAACCGCC+AGG + chr5.3:28298307-28298326 None:intergenic 55.0%
!! GACCGAGTTCTAAGACCTGG+CGG - chr5.3:28298289-28298308 MS.gene070562:CDS 55.0%
!! GCGTGGCAGAGAACTGGTTT+TGG - chr5.3:28297108-28297127 MS.gene070562:CDS 55.0%
CCTAGGACACCAATGACGGC+AGG + chr5.3:28298079-28298098 None:intergenic 60.0%
GCACGCCGTTGATCATGGCA+GGG + chr5.3:28297303-28297322 None:intergenic 60.0%
GGAATTGGGTTCCACCACCG+GGG + chr5.3:28297548-28297567 None:intergenic 60.0%
GGGAATTGGGTTCCACCACC+GGG + chr5.3:28297549-28297568 None:intergenic 60.0%
GTGTTCAGATTCCCCGGTGG+TGG - chr5.3:28297534-28297553 MS.gene070562:intron 60.0%
! AGGGGTGTACCTGCCGTCAT+TGG - chr5.3:28298067-28298086 MS.gene070562:CDS 60.0%
! CCTGCCGTCATTGGTGTCCT+AGG - chr5.3:28298076-28298095 MS.gene070562:CDS 60.0%
! GTCAGGACAGCACTGGACAC+TGG - chr5.3:28297630-28297649 MS.gene070562:intron 60.0%
GGCACGCCGTTGATCATGGC+AGG + chr5.3:28297304-28297323 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 28297015 28300030 28297015 ID=MS.gene070562
chr5.3 mRNA 28297015 28300030 28297015 ID=MS.gene070562.t1;Parent=MS.gene070562
chr5.3 exon 28299386 28300030 28299386 ID=MS.gene070562.t1.exon1;Parent=MS.gene070562.t1
chr5.3 CDS 28299386 28300030 28299386 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
chr5.3 exon 28298867 28299077 28298867 ID=MS.gene070562.t1.exon2;Parent=MS.gene070562.t1
chr5.3 CDS 28298867 28299077 28298867 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
chr5.3 exon 28298485 28298779 28298485 ID=MS.gene070562.t1.exon3;Parent=MS.gene070562.t1
chr5.3 CDS 28298485 28298779 28298485 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
chr5.3 exon 28297942 28298310 28297942 ID=MS.gene070562.t1.exon4;Parent=MS.gene070562.t1
chr5.3 CDS 28297942 28298310 28297942 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
chr5.3 exon 28297458 28297535 28297458 ID=MS.gene070562.t1.exon5;Parent=MS.gene070562.t1
chr5.3 CDS 28297458 28297535 28297458 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
chr5.3 exon 28297015 28297246 28297015 ID=MS.gene070562.t1.exon6;Parent=MS.gene070562.t1
chr5.3 CDS 28297015 28297246 28297015 ID=cds.MS.gene070562.t1;Parent=MS.gene070562.t1
Gene Sequence

>MS.gene070562

ATGGCTAAACCAATTTCTTCTGGTAGTAGAACTAGAAGCTTTGTGCAGATCTTTATAGTAGTTGGTCTATGTTGTTTCTTCTACATATTAGGTGCGTGGCAGAGAACTGGTTTTGGAAAGGGAGATCTTTTACAATTGGAGGTTACTAAGAAAGGTGCTGGGTGTGATATTGTTCCAAATTTAAGCTTTGATTCTCACCATGGTGGAGAAGTTAGCAAAATTGATGAGGTTGATTCAAAACCTAAGGTATTTAAGCCTTGCAAGGCTCGATATATCGATTACACGCCCTGCCATGATCAACGGCGTGCCATGACCTTTTCTAGACAAAATATGATTTATCGAGAGAGGCATTGCCCTCGTGAAGAAGAGAAGTTACATTGTTTGATACCAGCACCGAAAGGGTATGTTACTCCATTTCCATGGCCAAAGAGCCGTGATTATGTTCCATATGCAAACGCACCCTACAAGAGTCTTACAGTGGAAAAGGCTATTCAGAACTGGATCCAATATGAGGGAAATGTGTTCAGATTCCCCGGTGGTGGAACCCAATTCCCTCAAGGGGCTGACAAATATATTGATCAAATTGCTTCTGTGACACCGATAAAGAATGGAACAGTCAGGACAGCACTGGACACTGGTTGTGGGGTACGTTTGTCATTGTGAATTCCTTAGGGCCTGTATCGTATTCTTTTGTCATTTTTGTTCTTGTTAATTTGATTAGCAGTATAGTTAGTTCAGGGTTGTCAAATAGTAGCTATGGTGCTATAGCATAGCAGTATTTGAACAAATCGCTATTGCTATGTAATACGCTATTTAGTACAAAGTGTTGTCAAATAGAGGCAGCACTGTAGTGGAATTTGAACAAACGAATATTTTCCATAATCCGCGACTAACATCACTGAGTCAGTTTGTTTTAGCTAGGTATCTGTAATGTGTAAGGTTGTTTTGCACAGGTTGCCAGTTGGGGTGCATATCTTTGGAGCAGAAATGTTATCACTATGTCATTTGCACCTAGGGACTCACATGAAGCACAAGTACAATTTGCACTTGAAAGGGGTGTACCTGCCGTCATTGGTGTCCTAGGAACCATAAAATTGCCTTATCCATCTGGAGCCTTTGATATGGCACATTGTTCTCGTTGCTTAATTCCTTGGGGATCAAATGGTGAGAAATTTACATTAAGAACTATAATTTTTCTTCTGAAGTACTTCGAGTAAATAACGAAAGTGCATCTTGGGTTGTTATTGGCAGATGGGATTTATATGATGGAAGTTGACCGAGTTCTAAGACCTGGCGGTTATTGGGTGCTTTCGGGACCTCCAATCCATTGGAAGGCTAATTACAAAGCCTGGCAAAGACCTAAGGAGGATCTTGAGGAAGAACAAAGAAAGATTGAAGAGGTTGCTAAGCTCCTTTGCTGGGAGAAGAAGACCGAGAAGAATGAAATTGCTGTCTGGCAGAAAACTATTGATTCTGAAACATGTCGAAGTAGACAAGAAGATTCTGGTGCGAAGTTCTGTGAATCAACAGATGCTGATGATGTTTGGTATGTGCTTTCTTTCAGTGTTAAACATTTTATGTATTTTAGCATCCATTGTTGATATTCCATTCATTTAGTTCTACTAACAAACTAGATCATTCGAGCATGCATAAATATAAGAAAAAAGTTGTGTACAAAAATCTTGGACTGAGTTGGAATGGATTTGTTTTGTCATTGCAGGTATAAGAAAATGGAAGCCTGTGTTACTCCTAATCGTAAAGTCCACGGAGATCTTAAGCCTTTTCCACAGAGACTATATGCAGTTCCTCCTAAAGTTGCCAGTGGTTCTGTTCCCGGAGTTTCAGCTGAGACATACCAGGATGATAAAGAAAAGTGGAAAAAACATGTAAATGCTTACAAGAAAATAAATAAGCTCCTGGACTCTGGAAGGTATCGCAATATCATGGATATGAATGCTGGCTTGGGTAGTTTTGCTGCTGCTATTCAATCTCCTAAGTTATGGGTCATGAATGTTGTGCCTACTATAGCTGAGAAACATACACTCGGTGTCATTTATCAGCGAGGACTCATAGGCATTTATCATGATTGGTATAGTTTTCTTACTTCTTCCTTCTATTTTATTCAGAGTGTCTTGATTTTATGTTTGAAAATGCTTAATCTTGTACTATTATTGTTTTGAGAAATGATACTTTGACATCAAAATTTGATTTCAAAATTCAAATACAATGTTCTTTGCATTTATGTGTATCTATGAAATGTGTGTAAATTGTAGAACATAAAAAGTAAGAGAAAGAGAGATAGAGATAAACATATACGGTGTCACATACGGTGTCTGTATTTTGATGTTCAAATAACACAACTCAATTCTTTTTCTCCTTGATGTATTGCATGAAAACGTAGACTCTATCTTGTATTGTTAGCACAACTTTCTGTGATCGGGTTTTGCCCTTTTCTCGCATGTTTTTCCTTATTTATTTATGCCTTTATGATCCAGGTGTGAAGCTTTCTCCACATATCCAAGGACGTACGACCTCATTCATAGCAATGGGCTCTTTAGTCTGTACAAGGACAAGTGAGTATTCCTTTAATTATATGGTTATTCAATTCTTAATCAATAGTTTAGCCTTTGCATAGAATGCATTATAAGTTGTATAGGTGGTATGAAAATGATTTCTAGACAACGATTATCAGTGATAAAACCACTCGGTTTTAGTTCTCTTTTTCCCCTTCGTCAGTTGTGTTCATTCTTGTCTCAAAATCCTGTATCTAATCATCAAATGCAGATGTAACATAGAAGACATTCTTCTGGAGATGGACCGGATTTTACGACCTGAAGGTGCTGTTATATTCCGCGATGAAGTTGATATCTTAATCAAGGTAAAGAAAATTGTTGGAGGAATGCGATGGGATACTAAACTGGTCGACCATGAGGATGGTCCACTTGTTCCTGAAAAGATACTAATTGCTGTCAAGCAGTATTGGGTTGCTGATACAAATACCACGTCAACACAATAA
Protein sequence

>MS.gene070562.t1

MAKPISSGSRTRSFVQIFIVVGLCCFFYILGAWQRTGFGKGDLLQLEVTKKGAGCDIVPNLSFDSHHGGEVSKIDEVDSKPKVFKPCKARYIDYTPCHDQRRAMTFSRQNMIYRERHCPREEEKLHCLIPAPKGYVTPFPWPKSRDYVPYANAPYKSLTVEKAIQNWIQYEGNVFRFPGGGTQFPQGADKYIDQIASVTPIKNGTVRTALDTGCGVASWGAYLWSRNVITMSFAPRDSHEAQVQFALERGVPAVIGVLGTIKLPYPSGAFDMAHCSRCLIPWGSNDGIYMMEVDRVLRPGGYWVLSGPPIHWKANYKAWQRPKEDLEEEQRKIEEVAKLLCWEKKTEKNEIAVWQKTIDSETCRSRQEDSGAKFCESTDADDVWYKKMEACVTPNRKVHGDLKPFPQRLYAVPPKVASGSVPGVSAETYQDDKEKWKKHVNAYKKINKLLDSGRYRNIMDMNAGLGSFAAAIQSPKLWVMNVVPTIAEKHTLGVIYQRGLIGIYHDWCEAFSTYPRTYDLIHSNGLFSLYKDKCNIEDILLEMDRILRPEGAVIFRDEVDILIKVKKIVGGMRWDTKLVDHEDGPLVPEKILIAVKQYWVADTNTTSTQ