AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070714


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070714.t1 MTR_7g088160 43.730 311 136 3 18 303 75 371 5.66e-78 243
MS.gene070714.t1 MTR_4g063670 43.564 303 140 9 15 303 17 302 1.93e-55 182
MS.gene070714.t1 MTR_0867s0010 43.564 303 140 8 15 303 17 302 1.46e-51 172
MS.gene070714.t1 MTR_4g083510 45.370 216 96 7 15 226 17 214 2.52e-46 159
MS.gene070714.t1 MTR_7g107340 35.986 289 137 4 16 303 76 317 2.83e-44 154
MS.gene070714.t1 MTR_1g051185 47.020 151 70 4 77 225 45 187 3.56e-35 128
MS.gene070714.t1 MTR_3g069460 41.566 166 86 3 15 178 17 173 9.81e-35 124
MS.gene070714.t1 MTR_7g114170 38.333 180 68 5 16 195 61 197 3.69e-26 105
MS.gene070714.t1 MTR_6g081150 36.413 184 71 5 77 260 40 177 3.69e-25 99.4
MS.gene070714.t1 MTR_5g066280 25.188 266 176 3 20 283 55 299 3.25e-21 92.4
MS.gene070714.t1 MTR_4g026120 26.066 211 135 2 20 230 55 244 3.62e-21 92.0
MS.gene070714.t1 MTR_4g036435 26.066 211 135 2 20 230 55 244 3.62e-21 92.0
MS.gene070714.t1 MTR_3g067785 26.066 211 135 2 20 230 55 244 3.77e-21 92.0
MS.gene070714.t1 MTR_4g013220 26.066 211 135 2 20 230 55 244 4.69e-21 92.0
MS.gene070714.t1 MTR_1g106885 26.066 211 135 2 20 230 116 305 3.16e-20 90.1
MS.gene070714.t1 MTR_2g022120 26.066 211 135 2 20 230 119 308 3.23e-20 90.1
MS.gene070714.t1 MTR_5g077245 25.592 211 136 2 20 230 108 297 2.26e-19 87.8
MS.gene070714.t1 MTR_2g072860 28.659 164 112 2 18 181 29 187 5.39e-19 85.9
MS.gene070714.t1 MTR_7g461350 29.448 163 110 2 18 180 97 254 1.03e-18 85.9
MS.gene070714.t1 MTR_6g016670 29.448 163 110 2 18 180 97 254 1.03e-18 85.9
MS.gene070714.t1 MTR_3g464310 29.448 163 110 2 18 180 97 254 1.03e-18 85.9
MS.gene070714.t1 MTR_6g092533 27.329 161 114 1 20 180 119 276 1.11e-18 85.9
MS.gene070714.t1 MTR_3g464170 28.834 163 111 2 18 180 97 254 1.75e-18 85.1
MS.gene070714.t1 MTR_1g100375 28.834 163 111 2 18 180 97 254 1.75e-18 85.1
MS.gene070714.t1 MTR_6g051405 28.834 163 111 2 18 180 97 254 1.82e-18 85.1
MS.gene070714.t1 MTR_3g450670 28.221 163 112 2 18 180 93 250 4.04e-18 84.0
MS.gene070714.t1 MTR_1g471070 28.221 163 112 2 18 180 93 250 4.04e-18 84.0
MS.gene070714.t1 MTR_7g062380 23.977 171 112 1 59 229 26 178 4.69e-15 73.9
MS.gene070714.t1 MTR_6g453050 24.542 273 170 8 20 283 55 300 1.22e-13 70.5
MS.gene070714.t1 MTR_4g046090 26.316 152 94 1 79 230 71 204 8.17e-13 67.4
MS.gene070714.t1 MTR_7g083520 26.016 123 91 0 59 181 65 187 9.59e-13 67.8
MS.gene070714.t1 MTR_1g086750 27.966 118 85 0 59 176 26 143 1.76e-12 65.9
MS.gene070714.t1 MTR_8g015740 42.857 77 32 1 25 89 54 130 2.33e-11 60.8
MS.gene070714.t1 MTR_2g023560 63.636 44 16 0 58 101 62 105 3.41e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070714.t1 AT5G41220 38.889 198 108 4 50 238 206 399 9.13e-34 129
MS.gene070714.t1 AT5G41220 38.889 198 108 4 50 238 214 407 9.69e-34 129
MS.gene070714.t1 AT5G41220 38.889 198 108 4 50 238 264 457 1.29e-33 129
MS.gene070714.t1 AT5G41240 37.255 204 114 4 50 243 264 463 1.54e-32 126
MS.gene070714.t1 AT3G47680 32.558 215 117 8 22 227 14 209 1.14e-23 98.6
MS.gene070714.t1 AT1G66235 25.510 196 119 5 59 236 7 193 9.42e-13 67.8

Find 53 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGGATTCTTCAGTAAAT+TGG 0.187266 8.4:-1492942 None:intergenic
GTAAATTGGCATGACCATTT+AGG 0.268868 8.4:-1492928 None:intergenic
TAGAAATATTTAACCATGAT+TGG 0.280172 8.4:-1492609 None:intergenic
ATCATGGTTAAATATTTCTA+AGG 0.308295 8.4:+1492612 MS.gene070714:CDS
TACTAAAAGATGAACCTAAA+TGG 0.349471 8.4:+1492914 MS.gene070714:CDS
TTCACTTGAGTTGTATTGTC+CGG 0.371973 8.4:-1493037 None:intergenic
AGAACAAAGAATTCTGCTTC+AGG 0.392497 8.4:+1492967 MS.gene070714:CDS
AGAATTCTTTGTTCTCTTGT+TGG 0.394331 8.4:-1492959 None:intergenic
GTTAGATCCACATGAACAAT+AGG 0.411169 8.4:-1493129 None:intergenic
TCACTTGAGTTGTATTGTCC+GGG 0.435029 8.4:-1493036 None:intergenic
TCACCACTTGTAGGCGTGTC+CGG 0.441723 8.4:-1493012 None:intergenic
AGTAGAGTGGTTCGTCCGAT+TGG 0.444657 8.4:+1493060 MS.gene070714:CDS
ATCTCTTGATGGATACATCT+AGG 0.458045 8.4:+1493277 MS.gene070714:CDS
ATACACTTCTTATCCAATCA+TGG 0.460631 8.4:+1492596 MS.gene070714:CDS
AGGAGAAAATATATAGAGAA+AGG 0.462201 8.4:+1492557 MS.gene070714:CDS
TGAGCTTCACTATCTCTTGA+TGG 0.465437 8.4:+1493266 MS.gene070714:CDS
TTTGAAGAAATCTGAGCTGT+TGG 0.469253 8.4:-1492520 None:intergenic
ATGGTCGAAGGTGAACTTGA+TGG 0.477920 8.4:+1492750 MS.gene070714:CDS
CTACACATCCTCTTCTAATC+CGG 0.479246 8.4:+1492993 MS.gene070714:CDS
TGTCCGGGGTCACCACTTGT+AGG 0.492325 8.4:-1493021 None:intergenic
ATTTCTAAGGATCCAATTGT+GGG 0.492615 8.4:+1492625 MS.gene070714:CDS
GTAGAGTGGTTCGTCCGATT+GGG 0.497308 8.4:+1493061 MS.gene070714:CDS
CAATATCGTGGTCATTTGAA+AGG 0.504699 8.4:+1492700 MS.gene070714:CDS
TTGTTCATGTGGATCTAACT+AGG 0.507003 8.4:+1493133 MS.gene070714:CDS
GACAAAAGCAGCTAAAAGAA+AGG 0.508350 8.4:+1493083 MS.gene070714:CDS
TATTTCTAAGGATCCAATTG+TGG 0.512026 8.4:+1492624 MS.gene070714:CDS
CCAACTTGAGAGGAAAATGA+TGG 0.520919 8.4:-1492463 None:intergenic
GCATGAAGGTAACAAGTTGA+AGG 0.526954 8.4:+1493212 MS.gene070714:CDS
CGACATTATGCAAGATGCAT+TGG 0.532468 8.4:+1492837 MS.gene070714:CDS
AATATCGTGGTCATTTGAAA+GGG 0.541210 8.4:+1492701 MS.gene070714:CDS
ACAAAAGCAGCTAAAAGAAA+GGG 0.543467 8.4:+1493084 MS.gene070714:CDS
GCCTATTCAAGAAAGAAAAG+TGG 0.554764 8.4:+1492802 MS.gene070714:CDS
AGTTGGGTTAGATAATATTG+AGG 0.557841 8.4:+1492480 MS.gene070714:CDS
TGAATCAAATTAAATGTCGA+TGG 0.569722 8.4:+1492731 MS.gene070714:CDS
TCATGTGGATCTAACTAGGA+TGG 0.578161 8.4:+1493137 MS.gene070714:CDS
TGAAACACCTATTGTTCATG+TGG 0.578577 8.4:+1493122 MS.gene070714:CDS
AATTAAATGTCGATGGTCGA+AGG 0.587022 8.4:+1492738 MS.gene070714:CDS
CGGATTAGAAGAGGATGTGT+AGG 0.590924 8.4:-1492992 None:intergenic
ATTATCTAACCCAACTTGAG+AGG 0.596041 8.4:-1492473 None:intergenic
TTCTAAGGATCCAATTGTGG+GGG 0.599880 8.4:+1492627 MS.gene070714:CDS
ACGCCTACAAGTGGTGACCC+CGG 0.604163 8.4:+1493018 MS.gene070714:CDS
CACTTGAGTTGTATTGTCCG+GGG 0.607801 8.4:-1493035 None:intergenic
ATTGCACGTGGTGTGCATGA+AGG 0.607967 8.4:+1493198 MS.gene070714:CDS
GATAATTATAATCAATATCG+TGG 0.614936 8.4:+1492688 MS.gene070714:CDS
AATCCGGACACGCCTACAAG+TGG 0.635438 8.4:+1493009 MS.gene070714:CDS
AGGCGTGTCCGGATTAGAAG+AGG 0.638071 8.4:-1493001 None:intergenic
TTTCTAAGGATCCAATTGTG+GGG 0.639337 8.4:+1492626 MS.gene070714:CDS
CAACTCAAGTGAAAGTAGAG+TGG 0.640911 8.4:+1493047 MS.gene070714:CDS
ATATAGAGAAAGGTTTACCA+CGG 0.696539 8.4:+1492567 MS.gene070714:CDS
GCTTTAGAAGATATTGCACG+TGG 0.699659 8.4:+1493186 MS.gene070714:CDS
GGATACATCTAGGATGACAG+AGG 0.702928 8.4:+1493287 MS.gene070714:CDS
AAGAAGTGTATCTTCCTCCG+TGG 0.715732 8.4:-1492584 None:intergenic
TAGAGAAAGGTTTACCACGG+AGG 0.724762 8.4:+1492570 MS.gene070714:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAATTATAATCAATATCG+TGG + chr8.4:1492688-1492707 MS.gene070714:CDS 20.0%
!! TAGAAATATTTAACCATGAT+TGG - chr8.4:1492612-1492631 None:intergenic 20.0%
!! TTCAAAAGAAACAAAAACAA+AGG + chr8.4:1492537-1492556 MS.gene070714:CDS 20.0%
!!! ATCATGGTTAAATATTTCTA+AGG + chr8.4:1492612-1492631 MS.gene070714:CDS 20.0%
! AGGAGAAAATATATAGAGAA+AGG + chr8.4:1492557-1492576 MS.gene070714:CDS 25.0%
! GGAAAAAGAATTGCAAATTA+AGG + chr8.4:1493233-1493252 MS.gene070714:CDS 25.0%
! TACTAAAAGATGAACCTAAA+TGG + chr8.4:1492914-1492933 MS.gene070714:CDS 25.0%
! TGAATCAAATTAAATGTCGA+TGG + chr8.4:1492731-1492750 MS.gene070714:CDS 25.0%
AATATCGTGGTCATTTGAAA+GGG + chr8.4:1492701-1492720 MS.gene070714:CDS 30.0%
ACAAAAGCAGCTAAAAGAAA+GGG + chr8.4:1493084-1493103 MS.gene070714:CDS 30.0%
AGAATTCTTTGTTCTCTTGT+TGG - chr8.4:1492962-1492981 None:intergenic 30.0%
ATACACTTCTTATCCAATCA+TGG + chr8.4:1492596-1492615 MS.gene070714:CDS 30.0%
ATATAGAGAAAGGTTTACCA+CGG + chr8.4:1492567-1492586 MS.gene070714:CDS 30.0%
TGTTGGATTCTTCAGTAAAT+TGG - chr8.4:1492945-1492964 None:intergenic 30.0%
! AGTTGGGTTAGATAATATTG+AGG + chr8.4:1492480-1492499 MS.gene070714:CDS 30.0%
! ATTTCTAAGGATCCAATTGT+GGG + chr8.4:1492625-1492644 MS.gene070714:CDS 30.0%
! TATTTCTAAGGATCCAATTG+TGG + chr8.4:1492624-1492643 MS.gene070714:CDS 30.0%
! TCTTTTAGTAATTTCCATGC+TGG - chr8.4:1492904-1492923 None:intergenic 30.0%
!! ACCACTTTTCTTTCTTGAAT+AGG - chr8.4:1492806-1492825 None:intergenic 30.0%
AATTAAATGTCGATGGTCGA+AGG + chr8.4:1492738-1492757 MS.gene070714:CDS 35.0%
AGAACAAAGAATTCTGCTTC+AGG + chr8.4:1492967-1492986 MS.gene070714:CDS 35.0%
ATTATCTAACCCAACTTGAG+AGG - chr8.4:1492476-1492495 None:intergenic 35.0%
CAATATCGTGGTCATTTGAA+AGG + chr8.4:1492700-1492719 MS.gene070714:CDS 35.0%
GACAAAAGCAGCTAAAAGAA+AGG + chr8.4:1493083-1493102 MS.gene070714:CDS 35.0%
GCCTATTCAAGAAAGAAAAG+TGG + chr8.4:1492802-1492821 MS.gene070714:CDS 35.0%
GTAAATTGGCATGACCATTT+AGG - chr8.4:1492931-1492950 None:intergenic 35.0%
GTTAGATCCACATGAACAAT+AGG - chr8.4:1493132-1493151 None:intergenic 35.0%
TGAAACACCTATTGTTCATG+TGG + chr8.4:1493122-1493141 MS.gene070714:CDS 35.0%
TTGTTCATGTGGATCTAACT+AGG + chr8.4:1493133-1493152 MS.gene070714:CDS 35.0%
TTTGAAGAAATCTGAGCTGT+TGG - chr8.4:1492523-1492542 None:intergenic 35.0%
! ACCAAAGAGCAAGTTCTTTT+TGG + chr8.4:1492653-1492672 MS.gene070714:CDS 35.0%
! ATCTCTTGATGGATACATCT+AGG + chr8.4:1493277-1493296 MS.gene070714:CDS 35.0%
! CATCATTTTCCTCTCAAGTT+GGG + chr8.4:1492464-1492483 MS.gene070714:CDS 35.0%
! TCCAAAAAGAACTTGCTCTT+TGG - chr8.4:1492657-1492676 None:intergenic 35.0%
! TTCACTTGAGTTGTATTGTC+CGG - chr8.4:1493040-1493059 None:intergenic 35.0%
! TTTCTAAGGATCCAATTGTG+GGG + chr8.4:1492626-1492645 MS.gene070714:CDS 35.0%
CAACTCAAGTGAAAGTAGAG+TGG + chr8.4:1493047-1493066 MS.gene070714:CDS 40.0%
CCAACTTGAGAGGAAAATGA+TGG - chr8.4:1492466-1492485 None:intergenic 40.0%
CGACATTATGCAAGATGCAT+TGG + chr8.4:1492837-1492856 MS.gene070714:CDS 40.0%
CTACACATCCTCTTCTAATC+CGG + chr8.4:1492993-1493012 MS.gene070714:CDS 40.0%
TCATGTGGATCTAACTAGGA+TGG + chr8.4:1493137-1493156 MS.gene070714:CDS 40.0%
TGAGCTTCACTATCTCTTGA+TGG + chr8.4:1493266-1493285 MS.gene070714:CDS 40.0%
! CCATCATTTTCCTCTCAAGT+TGG + chr8.4:1492463-1492482 MS.gene070714:CDS 40.0%
! GCATGAAGGTAACAAGTTGA+AGG + chr8.4:1493212-1493231 MS.gene070714:CDS 40.0%
! GTTTTATGTTAGTGCCAGCA+TGG + chr8.4:1492887-1492906 MS.gene070714:CDS 40.0%
! TCACTTGAGTTGTATTGTCC+GGG - chr8.4:1493039-1493058 None:intergenic 40.0%
! TTCTAAGGATCCAATTGTGG+GGG + chr8.4:1492627-1492646 MS.gene070714:CDS 40.0%
!! GCTTTAGAAGATATTGCACG+TGG + chr8.4:1493186-1493205 MS.gene070714:CDS 40.0%
!!! TTAGCTGCTTTTGTCCCAAT+CGG - chr8.4:1493078-1493097 None:intergenic 40.0%
ATGGTCGAAGGTGAACTTGA+TGG + chr8.4:1492750-1492769 MS.gene070714:CDS 45.0%
TAGAGAAAGGTTTACCACGG+AGG + chr8.4:1492570-1492589 MS.gene070714:CDS 45.0%
! AAGAAGTGTATCTTCCTCCG+TGG - chr8.4:1492587-1492606 None:intergenic 45.0%
! CACTTGAGTTGTATTGTCCG+GGG - chr8.4:1493038-1493057 None:intergenic 45.0%
! CGGATTAGAAGAGGATGTGT+AGG - chr8.4:1492995-1493014 None:intergenic 45.0%
! GGATACATCTAGGATGACAG+AGG + chr8.4:1493287-1493306 MS.gene070714:CDS 45.0%
AGTAGAGTGGTTCGTCCGAT+TGG + chr8.4:1493060-1493079 MS.gene070714:CDS 50.0%
ATTGCACGTGGTGTGCATGA+AGG + chr8.4:1493198-1493217 MS.gene070714:CDS 50.0%
GTAGAGTGGTTCGTCCGATT+GGG + chr8.4:1493061-1493080 MS.gene070714:CDS 50.0%
!! CTTTGGTTTGCCCCCACAAT+TGG - chr8.4:1492640-1492659 None:intergenic 50.0%
AATCCGGACACGCCTACAAG+TGG + chr8.4:1493009-1493028 MS.gene070714:CDS 55.0%
TCACCACTTGTAGGCGTGTC+CGG - chr8.4:1493015-1493034 None:intergenic 55.0%
! AGGCGTGTCCGGATTAGAAG+AGG - chr8.4:1493004-1493023 None:intergenic 55.0%
ACGCCTACAAGTGGTGACCC+CGG + chr8.4:1493018-1493037 MS.gene070714:CDS 60.0%
TGTCCGGGGTCACCACTTGT+AGG - chr8.4:1493024-1493043 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 1492406 1493314 1492406 ID=MS.gene070714
chr8.4 mRNA 1492406 1493314 1492406 ID=MS.gene070714.t1;Parent=MS.gene070714
chr8.4 exon 1492406 1493314 1492406 ID=MS.gene070714.t1.exon1;Parent=MS.gene070714.t1
chr8.4 CDS 1492406 1493314 1492406 ID=cds.MS.gene070714.t1;Parent=MS.gene070714.t1
Gene Sequence

>MS.gene070714

ATGAGTATTGCTACAGAACAAGAAACAACATCTCAAGCGTCTCAACAAATTCAAAATCCATCATTTTCCTCTCAAGTTGGGTTAGATAATATTGAGGTTAATGATGAAGAAGAACCAACAGCTCAGATTTCTTCAAAAGAAACAAAAACAAAGGAGAAAATATATAGAGAAAGGTTTACCACGGAGGAAGATACACTTCTTATCCAATCATGGTTAAATATTTCTAAGGATCCAATTGTGGGGGCAAACCAAAGAGCAAGTTCTTTTTGGAAGAGAATTACAGATAATTATAATCAATATCGTGGTCATTTGAAAGGGAGAGAGTTGAATCAAATTAAATGTCGATGGTCGAAGGTGAACTTGATGGTTCATAAATTTGCAACTTGCTACAAACAAGCCTATTCAAGAAAGAAAAGTGGTAGCTCTGAAGACGACATTATGCAAGATGCATTGGCAATTTATGCTCATGATAAGCGTTCACGTTTTATGTTAGTGCCAGCATGGAAATTACTAAAAGATGAACCTAAATGGTCATGCCAATTTACTGAAGAATCCAACAAGAGAACAAAGAATTCTGCTTCAGGAGCCTACACATCCTCTTCTAATCCGGACACGCCTACAAGTGGTGACCCCGGACAATACAACTCAAGTGAAAGTAGAGTGGTTCGTCCGATTGGGACAAAAGCAGCTAAAAGAAAGGGAAAATCAAAAGCAAATGAAACACCTATTGTTCATGTGGATCTAACTAGGATGGAAGATGCACAACAATCAAAAGCTCTAGCTTTAGAAGATATTGCACGTGGTGTGCATGAAGGTAACAAGTTGAAGGAAAAAGAATTGCAAATTAAGGAAAGAAATGCTGAGCTTCACTATCTCTTGATGGATACATCTAGGATGACAGAGGAACAA

Protein sequence

>MS.gene070714.t1

MSIATEQETTSQASQQIQNPSFSSQVGLDNIEVNDEEEPTAQISSKETKTKEKIYRERFTTEEDTLLIQSWLNISKDPIVGANQRASSFWKRITDNYNQYRGHLKGRELNQIKCRWSKVNLMVHKFATCYKQAYSRKKSGSSEDDIMQDALAIYAHDKRSRFMLVPAWKLLKDEPKWSCQFTEESNKRTKNSASGAYTSSSNPDTPTSGDPGQYNSSESRVVRPIGTKAAKRKGKSKANETPIVHVDLTRMEDAQQSKALALEDIARGVHEGNKLKEKELQIKERNAELHYLLMDTSRMTEEQ