Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070716.t1 | XP_027188200.1 | 47.5 | 305 | 145 | 3 | 18 | 321 | 26 | 316 | 1.10E-60 | 243.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070716.t1 | Q9FHE1 | 38.3 | 201 | 111 | 4 | 47 | 238 | 261 | 457 | 6.7e-27 | 122.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070716.t1 | A0A3Q7YDC8 | 47.5 | 305 | 145 | 3 | 18 | 321 | 26 | 316 | 7.8e-61 | 243.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene070716 | MS.gene90825 | PPI |
| MS.gene070716 | MS.gene97346 | PPI |
| MS.gene070716 | MS.gene029093 | PPI |
| MS.gene070716 | MS.gene066439 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070716.t1 | MTR_7g088160 | 43.769 | 329 | 146 | 3 | 18 | 321 | 75 | 389 | 1.58e-85 | 263 |
| MS.gene070716.t1 | MTR_4g063670 | 43.438 | 320 | 150 | 9 | 15 | 320 | 17 | 319 | 6.39e-62 | 200 |
| MS.gene070716.t1 | MTR_0867s0010 | 43.750 | 320 | 149 | 8 | 15 | 320 | 17 | 319 | 4.49e-59 | 192 |
| MS.gene070716.t1 | MTR_4g083510 | 40.683 | 322 | 157 | 10 | 15 | 320 | 17 | 320 | 3.31e-50 | 169 |
| MS.gene070716.t1 | MTR_7g107340 | 36.184 | 304 | 146 | 4 | 16 | 318 | 76 | 332 | 2.56e-49 | 167 |
| MS.gene070716.t1 | MTR_1g051185 | 47.020 | 151 | 70 | 4 | 77 | 225 | 45 | 187 | 4.82e-35 | 128 |
| MS.gene070716.t1 | MTR_3g069460 | 41.566 | 166 | 86 | 3 | 15 | 178 | 17 | 173 | 1.59e-34 | 124 |
| MS.gene070716.t1 | MTR_7g114170 | 33.663 | 303 | 125 | 8 | 16 | 318 | 61 | 287 | 1.91e-30 | 117 |
| MS.gene070716.t1 | MTR_6g081150 | 36.413 | 184 | 71 | 5 | 77 | 260 | 40 | 177 | 5.01e-25 | 99.8 |
| MS.gene070716.t1 | MTR_5g066280 | 24.503 | 302 | 200 | 5 | 20 | 319 | 55 | 330 | 4.34e-22 | 95.1 |
| MS.gene070716.t1 | MTR_4g026120 | 24.503 | 302 | 200 | 5 | 20 | 319 | 55 | 330 | 1.10e-21 | 94.0 |
| MS.gene070716.t1 | MTR_4g036435 | 24.503 | 302 | 200 | 5 | 20 | 319 | 55 | 330 | 1.10e-21 | 94.0 |
| MS.gene070716.t1 | MTR_3g067785 | 24.503 | 302 | 200 | 5 | 20 | 319 | 55 | 330 | 1.26e-21 | 94.0 |
| MS.gene070716.t1 | MTR_4g013220 | 24.503 | 302 | 200 | 5 | 20 | 319 | 55 | 330 | 1.36e-21 | 93.6 |
| MS.gene070716.t1 | MTR_1g106885 | 24.503 | 302 | 200 | 5 | 20 | 319 | 116 | 391 | 1.18e-20 | 92.0 |
| MS.gene070716.t1 | MTR_2g022120 | 24.503 | 302 | 200 | 5 | 20 | 319 | 119 | 394 | 1.19e-20 | 92.0 |
| MS.gene070716.t1 | MTR_5g077245 | 24.172 | 302 | 201 | 5 | 20 | 319 | 108 | 383 | 6.21e-20 | 89.7 |
| MS.gene070716.t1 | MTR_2g072860 | 28.659 | 164 | 112 | 2 | 18 | 181 | 29 | 187 | 5.84e-19 | 85.9 |
| MS.gene070716.t1 | MTR_7g461350 | 28.333 | 180 | 121 | 3 | 1 | 180 | 83 | 254 | 1.32e-18 | 85.9 |
| MS.gene070716.t1 | MTR_6g016670 | 28.333 | 180 | 121 | 3 | 1 | 180 | 83 | 254 | 1.32e-18 | 85.9 |
| MS.gene070716.t1 | MTR_3g464310 | 28.333 | 180 | 121 | 3 | 1 | 180 | 83 | 254 | 1.32e-18 | 85.9 |
| MS.gene070716.t1 | MTR_6g092533 | 27.329 | 161 | 114 | 1 | 20 | 180 | 119 | 276 | 1.48e-18 | 85.9 |
| MS.gene070716.t1 | MTR_3g464170 | 27.778 | 180 | 122 | 3 | 1 | 180 | 83 | 254 | 2.18e-18 | 85.1 |
| MS.gene070716.t1 | MTR_1g100375 | 27.778 | 180 | 122 | 3 | 1 | 180 | 83 | 254 | 2.18e-18 | 85.1 |
| MS.gene070716.t1 | MTR_6g051405 | 28.834 | 163 | 111 | 2 | 18 | 180 | 97 | 254 | 2.40e-18 | 85.1 |
| MS.gene070716.t1 | MTR_3g450670 | 28.221 | 163 | 112 | 2 | 18 | 180 | 93 | 250 | 5.96e-18 | 84.0 |
| MS.gene070716.t1 | MTR_1g471070 | 28.221 | 163 | 112 | 2 | 18 | 180 | 93 | 250 | 5.96e-18 | 84.0 |
| MS.gene070716.t1 | MTR_7g062380 | 23.626 | 182 | 121 | 1 | 48 | 229 | 15 | 178 | 5.37e-15 | 73.9 |
| MS.gene070716.t1 | MTR_6g453050 | 24.540 | 163 | 116 | 3 | 20 | 180 | 55 | 212 | 1.66e-13 | 70.5 |
| MS.gene070716.t1 | MTR_8g015750 | 33.654 | 208 | 102 | 6 | 125 | 320 | 3 | 186 | 3.81e-13 | 67.4 |
| MS.gene070716.t1 | MTR_4g046090 | 26.316 | 152 | 94 | 1 | 79 | 230 | 71 | 204 | 6.41e-13 | 68.2 |
| MS.gene070716.t1 | MTR_7g083520 | 23.125 | 160 | 120 | 1 | 22 | 181 | 31 | 187 | 1.23e-12 | 67.8 |
| MS.gene070716.t1 | MTR_4g046740 | 35.659 | 129 | 64 | 5 | 196 | 320 | 23 | 136 | 1.69e-12 | 64.3 |
| MS.gene070716.t1 | MTR_1g086750 | 27.966 | 118 | 85 | 0 | 59 | 176 | 26 | 143 | 2.08e-12 | 65.9 |
| MS.gene070716.t1 | MTR_8g015740 | 42.857 | 77 | 32 | 1 | 25 | 89 | 54 | 130 | 2.97e-11 | 60.5 |
| MS.gene070716.t1 | MTR_2g023560 | 63.636 | 44 | 16 | 0 | 58 | 101 | 62 | 105 | 3.16e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070716.t1 | AT5G41220 | 38.889 | 198 | 108 | 4 | 50 | 238 | 214 | 407 | 1.35e-33 | 129 |
| MS.gene070716.t1 | AT5G41220 | 38.889 | 198 | 108 | 4 | 50 | 238 | 206 | 399 | 1.36e-33 | 129 |
| MS.gene070716.t1 | AT5G41220 | 38.889 | 198 | 108 | 4 | 50 | 238 | 264 | 457 | 2.27e-33 | 129 |
| MS.gene070716.t1 | AT5G41240 | 37.624 | 202 | 112 | 4 | 50 | 241 | 264 | 461 | 2.86e-32 | 126 |
| MS.gene070716.t1 | AT3G47680 | 32.558 | 215 | 117 | 8 | 22 | 227 | 14 | 209 | 1.46e-23 | 98.6 |
| MS.gene070716.t1 | AT1G66235 | 25.510 | 196 | 119 | 5 | 59 | 236 | 7 | 193 | 1.10e-12 | 67.8 |
Find 0 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATTATAATCAATATCG+TGG | + | 32203:8226-8245 | MS.gene070716:CDS | 20.0% |
| !! | TAGAAATATTTAACCATGAT+TGG | - | 32203:8150-8169 | MS.gene070716:intergenic | 20.0% |
| !! | TTCAAAAGAAACAAAAACAA+AGG | + | 32203:8075-8094 | MS.gene070716:CDS | 20.0% |
| !!! | ATCATGGTTAAATATTTCTA+AGG | + | 32203:8150-8169 | MS.gene070716:CDS | 20.0% |
| ! | AGGAGAAAATATATAGAGAA+AGG | + | 32203:8095-8114 | MS.gene070716:CDS | 25.0% |
| ! | GGAAAAAGAATTGCAAATTA+AGG | + | 32203:8771-8790 | MS.gene070716:CDS | 25.0% |
| ! | TACTAAAAGATGAACCTAAA+TGG | + | 32203:8452-8471 | MS.gene070716:CDS | 25.0% |
| ! | TGAATCAAATTAAATGTCGA+TGG | + | 32203:8269-8288 | MS.gene070716:CDS | 25.0% |
| AATATCGTGGTCATTTGAAA+GGG | + | 32203:8239-8258 | MS.gene070716:CDS | 30.0% | |
| ACAAAAGCAGCTAAAAGAAA+GGG | + | 32203:8622-8641 | MS.gene070716:CDS | 30.0% | |
| AGAATTCTTTGTTCTCTTGT+TGG | - | 32203:8500-8519 | MS.gene070716:intergenic | 30.0% | |
| ATACACTTCTTATCCAATCA+TGG | + | 32203:8134-8153 | MS.gene070716:CDS | 30.0% | |
| ATATAGAGAAAGGTTTACCA+CGG | + | 32203:8105-8124 | MS.gene070716:CDS | 30.0% | |
| TGTTGGATTCTTCAGTAAAT+TGG | - | 32203:8483-8502 | MS.gene070716:intergenic | 30.0% | |
| ! | AGTTGGGTTAGATAATATTG+AGG | + | 32203:8018-8037 | MS.gene070716:CDS | 30.0% |
| ! | ATTTCTAAGGATCCAATTGT+GGG | + | 32203:8163-8182 | MS.gene070716:CDS | 30.0% |
| ! | TATTTCTAAGGATCCAATTG+TGG | + | 32203:8162-8181 | MS.gene070716:CDS | 30.0% |
| ! | TCTTTTAGTAATTTCCATGC+TGG | - | 32203:8442-8461 | MS.gene070716:intergenic | 30.0% |
| !! | ACCACTTTTCTTTCTTGAAT+AGG | - | 32203:8344-8363 | MS.gene070716:intergenic | 30.0% |
| AATTAAATGTCGATGGTCGA+AGG | + | 32203:8276-8295 | MS.gene070716:CDS | 35.0% | |
| AGAACAAAGAATTCTGCTTC+AGG | + | 32203:8505-8524 | MS.gene070716:CDS | 35.0% | |
| ATTATCTAACCCAACTTGAG+AGG | - | 32203:8014-8033 | MS.gene070716:intergenic | 35.0% | |
| CAATATCGTGGTCATTTGAA+AGG | + | 32203:8238-8257 | MS.gene070716:CDS | 35.0% | |
| GACAAAAGCAGCTAAAAGAA+AGG | + | 32203:8621-8640 | MS.gene070716:CDS | 35.0% | |
| GCCTATTCAAGAAAGAAAAG+TGG | + | 32203:8340-8359 | MS.gene070716:CDS | 35.0% | |
| GTAAATTGGCATGACCATTT+AGG | - | 32203:8469-8488 | MS.gene070716:intergenic | 35.0% | |
| GTTAGATCCACATGAACAAT+AGG | - | 32203:8670-8689 | MS.gene070716:intergenic | 35.0% | |
| TGAAACACCTATTGTTCATG+TGG | + | 32203:8660-8679 | MS.gene070716:CDS | 35.0% | |
| TTGTTCATGTGGATCTAACT+AGG | + | 32203:8671-8690 | MS.gene070716:CDS | 35.0% | |
| TTTGAAGAAATCTGAGCTGT+TGG | - | 32203:8061-8080 | MS.gene070716:intergenic | 35.0% | |
| ! | ACCAAAGAGCAAGTTCTTTT+TGG | + | 32203:8191-8210 | MS.gene070716:CDS | 35.0% |
| ! | ATCTCTTGATGGATACATCT+AGG | + | 32203:8815-8834 | MS.gene070716:CDS | 35.0% |
| ! | CATCATTTTCCTCTCAAGTT+GGG | + | 32203:8002-8021 | MS.gene070716:CDS | 35.0% |
| ! | TCCAAAAAGAACTTGCTCTT+TGG | - | 32203:8195-8214 | MS.gene070716:intergenic | 35.0% |
| ! | TTCACTTGAGTTGTATTGTC+CGG | - | 32203:8578-8597 | MS.gene070716:intergenic | 35.0% |
| ! | TTTCTAAGGATCCAATTGTG+GGG | + | 32203:8164-8183 | MS.gene070716:CDS | 35.0% |
| CAACTCAAGTGAAAGTAGAG+TGG | + | 32203:8585-8604 | MS.gene070716:CDS | 40.0% | |
| CCAACTTGAGAGGAAAATGA+TGG | - | 32203:8004-8023 | MS.gene070716:intergenic | 40.0% | |
| CGACATTATGCAAGATGCAT+TGG | + | 32203:8375-8394 | MS.gene070716:CDS | 40.0% | |
| CTACACATCCTCTTCTAATC+CGG | + | 32203:8531-8550 | MS.gene070716:CDS | 40.0% | |
| TCATGTGGATCTAACTAGGA+TGG | + | 32203:8675-8694 | MS.gene070716:CDS | 40.0% | |
| TGAGCTTCACTATCTCTTGA+TGG | + | 32203:8804-8823 | MS.gene070716:CDS | 40.0% | |
| ! | CCATCATTTTCCTCTCAAGT+TGG | + | 32203:8001-8020 | MS.gene070716:CDS | 40.0% |
| ! | GCATGAAGGTAACAAGTTGA+AGG | + | 32203:8750-8769 | MS.gene070716:CDS | 40.0% |
| ! | GTTTTATGTTAGTGCCAGCA+TGG | + | 32203:8425-8444 | MS.gene070716:CDS | 40.0% |
| ! | TCACTTGAGTTGTATTGTCC+GGG | - | 32203:8577-8596 | MS.gene070716:intergenic | 40.0% |
| ! | TTCTAAGGATCCAATTGTGG+GGG | + | 32203:8165-8184 | MS.gene070716:CDS | 40.0% |
| !! | GCTTTAGAAGATATTGCACG+TGG | + | 32203:8724-8743 | MS.gene070716:CDS | 40.0% |
| !!! | TTAGCTGCTTTTGTCCCAAT+CGG | - | 32203:8616-8635 | MS.gene070716:intergenic | 40.0% |
| ATGGTCGAAGGTGAACTTGA+TGG | + | 32203:8288-8307 | MS.gene070716:CDS | 45.0% | |
| TAGAGAAAGGTTTACCACGG+AGG | + | 32203:8108-8127 | MS.gene070716:CDS | 45.0% | |
| ! | AAGAAGTGTATCTTCCTCCG+TGG | - | 32203:8125-8144 | MS.gene070716:intergenic | 45.0% |
| ! | CACTTGAGTTGTATTGTCCG+GGG | - | 32203:8576-8595 | MS.gene070716:intergenic | 45.0% |
| ! | CGGATTAGAAGAGGATGTGT+AGG | - | 32203:8533-8552 | MS.gene070716:intergenic | 45.0% |
| ! | GGATACATCTAGGATGACAG+AGG | + | 32203:8825-8844 | MS.gene070716:CDS | 45.0% |
| AGTAGAGTGGTTCGTCCGAT+TGG | + | 32203:8598-8617 | MS.gene070716:CDS | 50.0% | |
| ATTGCACGTGGTGTGCATGA+AGG | + | 32203:8736-8755 | MS.gene070716:CDS | 50.0% | |
| GTAGAGTGGTTCGTCCGATT+GGG | + | 32203:8599-8618 | MS.gene070716:CDS | 50.0% | |
| !! | CTTTGGTTTGCCCCCACAAT+TGG | - | 32203:8178-8197 | MS.gene070716:intergenic | 50.0% |
| AATCCGGACACGCCTACAAG+TGG | + | 32203:8547-8566 | MS.gene070716:CDS | 55.0% | |
| TCACCACTTGTAGGCGTGTC+CGG | - | 32203:8553-8572 | MS.gene070716:intergenic | 55.0% | |
| ! | AGGCGTGTCCGGATTAGAAG+AGG | - | 32203:8542-8561 | MS.gene070716:intergenic | 55.0% |
| ACGCCTACAAGTGGTGACCC+CGG | + | 32203:8556-8575 | MS.gene070716:CDS | 60.0% | |
| TGTCCGGGGTCACCACTTGT+AGG | - | 32203:8562-8581 | MS.gene070716:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32203 | gene | 7944 | 8909 | 7944 | ID=MS.gene070716 |
| 32203 | mRNA | 7944 | 8909 | 7944 | ID=MS.gene070716.t1;Parent=MS.gene070716 |
| 32203 | exon | 7944 | 8909 | 7944 | ID=MS.gene070716.t1.exon1;Parent=MS.gene070716.t1 |
| 32203 | CDS | 7944 | 8909 | 7944 | ID=cds.MS.gene070716.t1;Parent=MS.gene070716.t1 |
| Gene Sequence |
| Protein sequence |
