Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070962.t1 | RHN81944.1 | 84.8 | 198 | 30 | 0 | 1 | 198 | 1 | 198 | 3.40E-81 | 311.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070962.t1 | P11432 | 76.3 | 198 | 45 | 2 | 1 | 198 | 1 | 196 | 1.6e-71 | 270.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070962.t1 | A0A396JXB1 | 84.8 | 198 | 30 | 0 | 1 | 198 | 1 | 198 | 2.4e-81 | 311.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05081 | MS.gene070962 | 0.839717 | 1.34E-57 | -1.69E-46 |
| MS.gene051838 | MS.gene070962 | 0.821036 | 5.00E-53 | -1.69E-46 |
| MS.gene051941 | MS.gene070962 | 0.811202 | 7.88E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene070962 | 0.823337 | 1.46E-53 | -1.69E-46 |
| MS.gene052870 | MS.gene070962 | 0.844663 | 6.58E-59 | -1.69E-46 |
| MS.gene052872 | MS.gene070962 | 0.85073 | 1.41E-60 | -1.69E-46 |
| MS.gene053824 | MS.gene070962 | 0.838985 | 2.08E-57 | -1.69E-46 |
| MS.gene054884 | MS.gene070962 | -0.806174 | 9.35E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene070962 | 0.837204 | 5.97E-57 | -1.69E-46 |
| MS.gene055224 | MS.gene070962 | 0.810655 | 1.03E-50 | -1.69E-46 |
| MS.gene055539 | MS.gene070962 | 0.824721 | 6.92E-54 | -1.69E-46 |
| MS.gene055741 | MS.gene070962 | 0.836319 | 1.00E-56 | -1.69E-46 |
| MS.gene055744 | MS.gene070962 | 0.832971 | 6.97E-56 | -1.69E-46 |
| MS.gene056877 | MS.gene070962 | 0.806414 | 8.32E-50 | -1.69E-46 |
| MS.gene057019 | MS.gene070962 | 0.809061 | 2.28E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene070962 | 0.801497 | 8.76E-49 | -1.69E-46 |
| MS.gene058338 | MS.gene070962 | 0.804469 | 2.13E-49 | -1.69E-46 |
| MS.gene058444 | MS.gene070962 | 0.8156 | 8.51E-52 | -1.69E-46 |
| MS.gene059023 | MS.gene070962 | 0.817848 | 2.67E-52 | -1.69E-46 |
| MS.gene061154 | MS.gene070962 | 0.801067 | 1.07E-48 | -1.69E-46 |
| MS.gene061231 | MS.gene070962 | 0.827837 | 1.25E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070962.t1 | MTR_1g102800 | 91.011 | 178 | 16 | 0 | 21 | 198 | 20 | 197 | 1.34e-110 | 314 |
| MS.gene070962.t1 | MTR_1g102570 | 89.899 | 198 | 20 | 0 | 1 | 198 | 1 | 198 | 2.68e-109 | 311 |
| MS.gene070962.t1 | MTR_1g102780 | 91.000 | 200 | 16 | 1 | 1 | 198 | 1 | 200 | 1.80e-104 | 299 |
| MS.gene070962.t1 | MTR_1g102830 | 79.397 | 199 | 27 | 2 | 1 | 198 | 1 | 186 | 7.76e-98 | 281 |
| MS.gene070962.t1 | MTR_1g102810 | 76.923 | 182 | 28 | 2 | 18 | 198 | 14 | 182 | 1.38e-97 | 281 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070962.t1 | AT3G22840 | 57.927 | 164 | 69 | 0 | 35 | 198 | 32 | 195 | 1.83e-60 | 187 |
| MS.gene070962.t1 | AT4G14690 | 56.140 | 171 | 74 | 1 | 29 | 198 | 23 | 193 | 2.62e-59 | 184 |
Find 45 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGATGATGATGGTGTAAT+TGG | 0.299898 | 1.4:-79507654 | None:intergenic |
| CCAGCACCTGAGAGAATTAA+TGG | 0.300603 | 1.4:+79507891 | MS.gene070962:CDS |
| GGTTTGTTTGATCAAATATC+TGG | 0.314167 | 1.4:+79507972 | MS.gene070962:CDS |
| TATGTTGGGTTTGATTGCTT+TGG | 0.357312 | 1.4:+79508136 | MS.gene070962:CDS |
| ATTGGGTCTACAGGCACCTT+TGG | 0.360861 | 1.4:-79507636 | None:intergenic |
| AATGGAAGGTTGGCTATGAT+TGG | 0.383969 | 1.4:+79507909 | MS.gene070962:CDS |
| TGGTGGAGTCCCATGGTTCT+TGG | 0.415020 | 1.4:+79507995 | MS.gene070962:CDS |
| CCATTAATTCTCTCAGGTGC+TGG | 0.425775 | 1.4:-79507891 | None:intergenic |
| GCTGGTTGTGGTGTTGGTGT+TGG | 0.432144 | 1.4:-79507675 | None:intergenic |
| GAATGGAAGAATAGCTATGT+TGG | 0.436992 | 1.4:+79508121 | MS.gene070962:CDS |
| AGCTCTAGCTCTGCTAGAAA+TGG | 0.453464 | 1.4:-79507366 | None:intergenic |
| AACCTTCCATTAATTCTCTC+AGG | 0.456054 | 1.4:-79507897 | None:intergenic |
| GTGATGATGATGGTGTAATT+GGG | 0.461935 | 1.4:-79507653 | None:intergenic |
| TGTCCTCAGATGCTGAGTTA+TGG | 0.479277 | 1.4:+79508099 | MS.gene070962:CDS |
| GTTGGTGTTGGTGATGATGA+TGG | 0.479914 | 1.4:-79507663 | None:intergenic |
| AATGGAAGAATAGCTATGTT+GGG | 0.501527 | 1.4:+79508122 | MS.gene070962:CDS |
| GTGTAGGCTGGTTGTGGTGT+TGG | 0.503331 | 1.4:-79507681 | None:intergenic |
| AGTGTGGAGTCTAAGTCAAA+AGG | 0.525000 | 1.4:+79508071 | MS.gene070962:CDS |
| AACCTTATTTACCTCAGCCA+TGG | 0.525798 | 1.4:-79507459 | None:intergenic |
| CACTTGTCGCCAAGAACCAT+GGG | 0.529249 | 1.4:-79508004 | None:intergenic |
| CTAGCATTCCTTCTGAAGCT+AGG | 0.532093 | 1.4:-79507422 | None:intergenic |
| TTTGGTGAGCGAGTGTAGGC+TGG | 0.536506 | 1.4:-79507693 | None:intergenic |
| GGTGGAAATAGCAAATGGAC+AGG | 0.544893 | 1.4:+79507950 | MS.gene070962:CDS |
| GAGCGAGTGTAGGCTGGTTG+TGG | 0.550985 | 1.4:-79507687 | None:intergenic |
| TTTGATCAAATATCTGGTGG+TGG | 0.551757 | 1.4:+79507978 | MS.gene070962:CDS |
| TGAGAGAATTAATGGAAGGT+TGG | 0.551924 | 1.4:+79507899 | MS.gene070962:CDS |
| GATGGTGTAATTGGGTCTAC+AGG | 0.568013 | 1.4:-79507645 | None:intergenic |
| TCAGATGCTGAGTTATGGAA+TGG | 0.568279 | 1.4:+79508104 | MS.gene070962:CDS |
| TTGTTTGATCAAATATCTGG+TGG | 0.570609 | 1.4:+79507975 | MS.gene070962:CDS |
| AAGCTAGGCATGTACACAGA+AGG | 0.579754 | 1.4:-79507407 | None:intergenic |
| ATTCCATAACTCAGCATCTG+AGG | 0.591329 | 1.4:-79508102 | None:intergenic |
| CTATAGAACTCTATTACACA+AGG | 0.596246 | 1.4:-79508187 | None:intergenic |
| TAGACTCCACACTAACACCT+TGG | 0.597870 | 1.4:-79508061 | None:intergenic |
| ACACTTGTCGCCAAGAACCA+TGG | 0.600374 | 1.4:-79508005 | None:intergenic |
| TAGCTTGAAAGTTCGATCCA+TGG | 0.604578 | 1.4:+79507442 | MS.gene070962:CDS |
| AGAGCAGAAAGAGCAACCAA+AGG | 0.611218 | 1.4:+79507620 | MS.gene070962:CDS |
| AGCCTACACTCGCTCACCAA+AGG | 0.611565 | 1.4:+79507695 | MS.gene070962:CDS |
| AGCTAGGCATGTACACAGAA+GGG | 0.614155 | 1.4:-79507406 | None:intergenic |
| GAAAGTTCGATCCATGGCTG+AGG | 0.622095 | 1.4:+79507448 | MS.gene070962:CDS |
| TGTACATGCCTAGCTTCAGA+AGG | 0.622254 | 1.4:+79507414 | MS.gene070962:CDS |
| CACCTGAGAGAATTAATGGA+AGG | 0.651384 | 1.4:+79507895 | MS.gene070962:CDS |
| TATCTGGTGGTGGAGTCCCA+TGG | 0.653372 | 1.4:+79507988 | MS.gene070962:CDS |
| TACCTTTGGTGAGCGAGTGT+AGG | 0.653559 | 1.4:-79507697 | None:intergenic |
| GTGGAAATAGCAAATGGACA+GGG | 0.661963 | 1.4:+79507951 | MS.gene070962:CDS |
| TATAGAACTCTATTACACAA+GGG | 0.699610 | 1.4:-79508186 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATATGTTTATGATTTAT+TGG | + | chr1.4:79507572-79507591 | MS.gene070962:intron | 10.0% |
| !! | TAATAATAATGATGAAAATA+TGG | + | chr1.4:79507826-79507845 | MS.gene070962:intron | 10.0% |
| !! | TATAAGATAAACAAAATTTA+AGG | + | chr1.4:79507482-79507501 | MS.gene070962:intron | 10.0% |
| !! | AAATCATAAACATATTATGT+TGG | - | chr1.4:79507570-79507589 | None:intergenic | 15.0% |
| !!! | ATTTTGCTAAGTACTAAATT+AGG | + | chr1.4:79507776-79507795 | MS.gene070962:intron | 20.0% |
| AATGGAAGAATAGCTATGTT+GGG | + | chr1.4:79508122-79508141 | MS.gene070962:CDS | 30.0% | |
| TTGTTTGATCAAATATCTGG+TGG | + | chr1.4:79507975-79507994 | MS.gene070962:CDS | 30.0% | |
| TTTGTTACTATGCATACCTT+TGG | - | chr1.4:79507714-79507733 | None:intergenic | 30.0% | |
| ! | GCTTTTACAGAGTATGTTAA+GGG | + | chr1.4:79508158-79508177 | MS.gene070962:CDS | 30.0% |
| ! | GGTTTGTTTGATCAAATATC+TGG | + | chr1.4:79507972-79507991 | MS.gene070962:CDS | 30.0% |
| !! | TATTATGTTGGTGATGATGA+AGG | - | chr1.4:79507558-79507577 | None:intergenic | 30.0% |
| !! | TCTTTGATTCCATTTTTCCA+AGG | + | chr1.4:79508044-79508063 | MS.gene070962:CDS | 30.0% |
| AACCTTCCATTAATTCTCTC+AGG | - | chr1.4:79507900-79507919 | None:intergenic | 35.0% | |
| ACACTAACACCTTGGAAAAA+TGG | - | chr1.4:79508056-79508075 | None:intergenic | 35.0% | |
| GAATGGAAGAATAGCTATGT+TGG | + | chr1.4:79508121-79508140 | MS.gene070962:CDS | 35.0% | |
| TATGTTGGGTTTGATTGCTT+TGG | + | chr1.4:79508136-79508155 | MS.gene070962:CDS | 35.0% | |
| TGAGAGAATTAATGGAAGGT+TGG | + | chr1.4:79507899-79507918 | MS.gene070962:CDS | 35.0% | |
| TTTGATCAAATATCTGGTGG+TGG | + | chr1.4:79507978-79507997 | MS.gene070962:CDS | 35.0% | |
| ! | CACAAAGTTTTCGGATTTGA+TGG | + | chr1.4:79507857-79507876 | MS.gene070962:CDS | 35.0% |
| ! | GGCTTTTACAGAGTATGTTA+AGG | + | chr1.4:79508157-79508176 | MS.gene070962:CDS | 35.0% |
| !! | ATTTTTCCAAGGTGTTAGTG+TGG | + | chr1.4:79508055-79508074 | MS.gene070962:CDS | 35.0% |
| !! | GTGATGATGATGGTGTAATT+GGG | - | chr1.4:79507656-79507675 | None:intergenic | 35.0% |
| !! | TTTTCGGATTTGATGGCTTT+TGG | + | chr1.4:79507864-79507883 | MS.gene070962:CDS | 35.0% |
| !!! | ATTGGTTTTGTAGCTGCAAT+GGG | + | chr1.4:79507927-79507946 | MS.gene070962:CDS | 35.0% |
| AGTGTGGAGTCTAAGTCAAA+AGG | + | chr1.4:79508071-79508090 | MS.gene070962:CDS | 40.0% | |
| ATCCATGGCTGAGGTAAATA+AGG | + | chr1.4:79507457-79507476 | MS.gene070962:intron | 40.0% | |
| ATTCCATAACTCAGCATCTG+AGG | - | chr1.4:79508105-79508124 | None:intergenic | 40.0% | |
| CACCTGAGAGAATTAATGGA+AGG | + | chr1.4:79507895-79507914 | MS.gene070962:CDS | 40.0% | |
| GATTGCGTGTTACATCATTC+AGG | + | chr1.4:79507596-79507615 | MS.gene070962:intron | 40.0% | |
| GTGGAAATAGCAAATGGACA+GGG | + | chr1.4:79507951-79507970 | MS.gene070962:CDS | 40.0% | |
| TAGCTTGAAAGTTCGATCCA+TGG | + | chr1.4:79507442-79507461 | MS.gene070962:CDS | 40.0% | |
| TCAGATGCTGAGTTATGGAA+TGG | + | chr1.4:79508104-79508123 | MS.gene070962:CDS | 40.0% | |
| ! | AACCTTATTTACCTCAGCCA+TGG | - | chr1.4:79507462-79507481 | None:intergenic | 40.0% |
| ! | AATGGAAGGTTGGCTATGAT+TGG | + | chr1.4:79507909-79507928 | MS.gene070962:CDS | 40.0% |
| ! | GCAGATGAGCACAAAGTTTT+CGG | + | chr1.4:79507848-79507867 | MS.gene070962:intron | 40.0% |
| ! | GGTGATGATGAAGGTGTAAT+CGG | - | chr1.4:79507549-79507568 | None:intergenic | 40.0% |
| !! | GGTGATGATGATGGTGTAAT+TGG | - | chr1.4:79507657-79507676 | None:intergenic | 40.0% |
| !!! | GATTGGTTTTGTAGCTGCAA+TGG | + | chr1.4:79507926-79507945 | MS.gene070962:CDS | 40.0% |
| !!! | TTGGTTTTGTAGCTGCAATG+GGG | + | chr1.4:79507928-79507947 | MS.gene070962:CDS | 40.0% |
| AAGCTAGGCATGTACACAGA+AGG | - | chr1.4:79507410-79507429 | None:intergenic | 45.0% | |
| AGAGCAGAAAGAGCAACCAA+AGG | + | chr1.4:79507620-79507639 | MS.gene070962:CDS | 45.0% | |
| AGCTAGGCATGTACACAGAA+GGG | - | chr1.4:79507409-79507428 | None:intergenic | 45.0% | |
| ATGGGGGTGGAAATAGCAAA+TGG | + | chr1.4:79507945-79507964 | MS.gene070962:CDS | 45.0% | |
| CCAGCACCTGAGAGAATTAA+TGG | + | chr1.4:79507891-79507910 | MS.gene070962:CDS | 45.0% | |
| CCATTAATTCTCTCAGGTGC+TGG | - | chr1.4:79507894-79507913 | None:intergenic | 45.0% | |
| GATGGTGTAATTGGGTCTAC+AGG | - | chr1.4:79507648-79507667 | None:intergenic | 45.0% | |
| GGTGGAAATAGCAAATGGAC+AGG | + | chr1.4:79507950-79507969 | MS.gene070962:CDS | 45.0% | |
| TAGACTCCACACTAACACCT+TGG | - | chr1.4:79508064-79508083 | None:intergenic | 45.0% | |
| TGTACATGCCTAGCTTCAGA+AGG | + | chr1.4:79507414-79507433 | MS.gene070962:CDS | 45.0% | |
| TGTCCTCAGATGCTGAGTTA+TGG | + | chr1.4:79508099-79508118 | MS.gene070962:CDS | 45.0% | |
| !! | AGCTCTAGCTCTGCTAGAAA+TGG | - | chr1.4:79507369-79507388 | None:intergenic | 45.0% |
| !! | CGGATTTGATGGCTTTTGGT+GGG | + | chr1.4:79507868-79507887 | MS.gene070962:CDS | 45.0% |
| !! | CTAGCATTCCTTCTGAAGCT+AGG | - | chr1.4:79507425-79507444 | None:intergenic | 45.0% |
| !! | GTTGGTGTTGGTGATGATGA+TGG | - | chr1.4:79507666-79507685 | None:intergenic | 45.0% |
| !! | TCGGATTTGATGGCTTTTGG+TGG | + | chr1.4:79507867-79507886 | MS.gene070962:CDS | 45.0% |
| !!! | TGGTTTTGTAGCTGCAATGG+GGG | + | chr1.4:79507929-79507948 | MS.gene070962:CDS | 45.0% |
| !! | CATTATTATTATTTATTAAA+AGG | - | chr1.4:79507817-79507836 | None:intergenic | 5.0% |
| ACACTTGTCGCCAAGAACCA+TGG | - | chr1.4:79508008-79508027 | None:intergenic | 50.0% | |
| ATTGGGTCTACAGGCACCTT+TGG | - | chr1.4:79507639-79507658 | None:intergenic | 50.0% | |
| CACTTGTCGCCAAGAACCAT+GGG | - | chr1.4:79508007-79508026 | None:intergenic | 50.0% | |
| GAAAGTTCGATCCATGGCTG+AGG | + | chr1.4:79507448-79507467 | MS.gene070962:CDS | 50.0% | |
| TACCTTTGGTGAGCGAGTGT+AGG | - | chr1.4:79507700-79507719 | None:intergenic | 50.0% | |
| ! | TTTTGTAGCTGCAATGGGGG+TGG | + | chr1.4:79507932-79507951 | MS.gene070962:CDS | 50.0% |
| AGCCTACACTCGCTCACCAA+AGG | + | chr1.4:79507695-79507714 | MS.gene070962:CDS | 55.0% | |
| TGGTGGAGTCCCATGGTTCT+TGG | + | chr1.4:79507995-79508014 | MS.gene070962:CDS | 55.0% | |
| TTTGGTGAGCGAGTGTAGGC+TGG | - | chr1.4:79507696-79507715 | None:intergenic | 55.0% | |
| ! | GTGTAGGCTGGTTGTGGTGT+TGG | - | chr1.4:79507684-79507703 | None:intergenic | 55.0% |
| !! | GCTGGTTGTGGTGTTGGTGT+TGG | - | chr1.4:79507678-79507697 | None:intergenic | 55.0% |
| !! | TATCTGGTGGTGGAGTCCCA+TGG | + | chr1.4:79507988-79508007 | MS.gene070962:CDS | 55.0% |
| ! | GAGCGAGTGTAGGCTGGTTG+TGG | - | chr1.4:79507690-79507709 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 79507317 | 79508196 | 79507317 | ID=MS.gene070962 |
| chr1.4 | mRNA | 79507317 | 79508196 | 79507317 | ID=MS.gene070962.t1;Parent=MS.gene070962 |
| chr1.4 | exon | 79507317 | 79507469 | 79507317 | ID=MS.gene070962.t1.exon1;Parent=MS.gene070962.t1 |
| chr1.4 | CDS | 79507317 | 79507469 | 79507317 | ID=cds.MS.gene070962.t1;Parent=MS.gene070962.t1 |
| chr1.4 | exon | 79507618 | 79507716 | 79507618 | ID=MS.gene070962.t1.exon2;Parent=MS.gene070962.t1 |
| chr1.4 | CDS | 79507618 | 79507716 | 79507618 | ID=cds.MS.gene070962.t1;Parent=MS.gene070962.t1 |
| chr1.4 | exon | 79507852 | 79508196 | 79507852 | ID=MS.gene070962.t1.exon3;Parent=MS.gene070962.t1 |
| chr1.4 | CDS | 79507852 | 79508196 | 79507852 | ID=cds.MS.gene070962.t1;Parent=MS.gene070962.t1 |
| Gene Sequence |
| Protein sequence |
