Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071411.t1 | XP_003597930.2 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.40E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071411.t1 | P42322 | 37.8 | 172 | 100 | 2 | 1 | 167 | 1 | 170 | 1.6e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071411.t1 | B7FH75 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 2.5e-93 | 350.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene020092 | MS.gene071411 | PPI |
| MS.gene72809 | MS.gene071411 | PPI |
| MS.gene022826 | MS.gene071411 | PPI |
| MS.gene83474 | MS.gene071411 | PPI |
| MS.gene82121 | MS.gene071411 | PPI |
| MS.gene37035 | MS.gene071411 | PPI |
| MS.gene028111 | MS.gene071411 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071411.t1 | MTR_2g104130 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 5.87e-128 | 356 |
| MS.gene071411.t1 | MTR_4g130410 | 70.286 | 175 | 52 | 0 | 1 | 175 | 1 | 175 | 1.37e-91 | 264 |
| MS.gene071411.t1 | MTR_4g130410 | 71.429 | 161 | 46 | 0 | 1 | 161 | 1 | 161 | 5.36e-84 | 245 |
| MS.gene071411.t1 | MTR_1g076650 | 27.517 | 149 | 94 | 5 | 22 | 161 | 3 | 146 | 1.56e-11 | 59.7 |
| MS.gene071411.t1 | MTR_1g071150 | 27.517 | 149 | 95 | 5 | 21 | 161 | 2 | 145 | 2.35e-11 | 58.9 |
| MS.gene071411.t1 | MTR_1g041285 | 26.797 | 153 | 98 | 5 | 22 | 165 | 3 | 150 | 2.44e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071411.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071411.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071411.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071411.t1 | AT3G22930 | 27.152 | 151 | 96 | 5 | 24 | 165 | 28 | 173 | 5.16e-12 | 61.6 |
Find 23 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAGGTTCTTGGCCATTC+TGG | 0.182957 | 2.3:-806132 | MS.gene071411:intron |
| TGTACTAAATGCAGATAAAA+AGG | 0.239607 | 2.3:+807143 | None:intergenic |
| GACATTGGATGATTTCATTA+AGG | 0.296420 | 2.3:-806331 | MS.gene071411:intron |
| TTAAATTTGTCACAGGTTCT+TGG | 0.342309 | 2.3:-806141 | MS.gene071411:intron |
| TTGATTTCTCAGGAAGTCTT+AGG | 0.365914 | 2.3:-806396 | MS.gene071411:intron |
| AGGTTACTCAAGATGGTTGA+TGG | 0.393812 | 2.3:-807189 | MS.gene071411:CDS |
| TGTTATGAATCCACTTTCTC+AGG | 0.398536 | 2.3:-807558 | MS.gene071411:intron |
| GGTGTCTTTCAAAGACATAT+TGG | 0.407546 | 2.3:-806808 | MS.gene071411:CDS |
| ATTTGATTCAGTTTCTCAAC+AGG | 0.411656 | 2.3:-807672 | MS.gene071411:intron |
| GAAGTTCTAAAAGATTTGTC+TGG | 0.444669 | 2.3:-806786 | MS.gene071411:CDS |
| ATCCTGCTTCTTTCAGAACC+TGG | 0.474604 | 2.3:+806373 | None:intergenic |
| CAACTTGACAGAAATGCTAA+AGG | 0.478282 | 2.3:-807620 | MS.gene071411:CDS |
| TTTGCAGAGGTTACTCAAGA+TGG | 0.478552 | 2.3:-807196 | MS.gene071411:intron |
| TGATATGATACCTGAGAAAG+TGG | 0.501757 | 2.3:+807548 | None:intergenic |
| TCTGCATTTAGTACAAAAGC+TGG | 0.505330 | 2.3:-807135 | MS.gene071411:CDS |
| GCTAACTCAATATGATATTG+AGG | 0.520808 | 2.3:-807803 | MS.gene071411:CDS |
| TTCTCAGGAAGTCTTAGGCC+AGG | 0.525529 | 2.3:-806391 | MS.gene071411:intron |
| GGCCAGGTTCTGAAAGAAGC+AGG | 0.530644 | 2.3:-806375 | MS.gene071411:CDS |
| GTATATGATTCAGATCGTAA+TGG | 0.556045 | 2.3:-806834 | MS.gene071411:CDS |
| AAAAGACTGCTACTTGACAT+TGG | 0.563411 | 2.3:-806346 | MS.gene071411:CDS |
| AGTGGATTCATAACAAACTC+AGG | 0.575670 | 2.3:+807566 | None:intergenic |
| TGATTCAGATCGTAATGGAA+AGG | 0.613377 | 2.3:-806829 | MS.gene071411:CDS |
| CATGTCTGATGATCAAAGAG+AGG | 0.654475 | 2.3:-806757 | MS.gene071411:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTATCTAAATTATTT+AGG | + | chr2.3:806919-806938 | None:intergenic | 10.0% |
| !! | TTTATATTTAAATTTGTCAC+AGG | - | chr2.3:807773-807792 | MS.gene071411:intron | 15.0% |
| !!! | ATTTTCTTATATATCTCTTT+TGG | - | chr2.3:807620-807639 | MS.gene071411:CDS | 15.0% |
| !!! | TTTTATGAAAATGGTATATT+TGG | + | chr2.3:806555-806574 | None:intergenic | 15.0% |
| !!! | TTTTATTCTTTATTAGTGTT+TGG | - | chr2.3:806867-806886 | MS.gene071411:intron | 15.0% |
| !!! | TTTTATTGGCTTTTATTTAA+TGG | + | chr2.3:807424-807443 | None:intergenic | 15.0% |
| !! | ACACATTTATTTAATTGAGT+CGG | + | chr2.3:806487-806506 | None:intergenic | 20.0% |
| !! | ACTCAATTAAATAAATGTGT+TGG | - | chr2.3:806487-806506 | MS.gene071411:intron | 20.0% |
| !! | ATCATAAATTAGAAGATTTC+AGG | - | chr2.3:806933-806952 | MS.gene071411:intron | 20.0% |
| !! | GAAATGAAATATTCGTATAT+AGG | + | chr2.3:807402-807421 | None:intergenic | 20.0% |
| !!! | ATGTGTTTACTATTTTATAC+AGG | - | chr2.3:806657-806676 | MS.gene071411:intron | 20.0% |
| !!! | GTTCTTTCTAATTGTAATTT+TGG | - | chr2.3:807728-807747 | MS.gene071411:intron | 20.0% |
| !!! | TGTGGAAAAAAGTTTTTTAT+TGG | + | chr2.3:807438-807457 | None:intergenic | 20.0% |
| !!! | TTTGAATTTTAAAGACTTTG+TGG | - | chr2.3:806755-806774 | MS.gene071411:intron | 20.0% |
| !!! | TTTTTTTGAAGAAACTCTTT+TGG | - | chr2.3:807658-807677 | MS.gene071411:CDS | 20.0% |
| ! | ACATTTATTTAATTGAGTCG+GGG | + | chr2.3:806485-806504 | None:intergenic | 25.0% |
| ! | ACTATAAGTACCCTAATTAA+AGG | + | chr2.3:807034-807053 | None:intergenic | 25.0% |
| ! | AGAAGAAGAAGAAAAAGATA+AGG | + | chr2.3:806189-806208 | None:intergenic | 25.0% |
| ! | AGTATATCAAACTCAACATA+GGG | + | chr2.3:807309-807328 | None:intergenic | 25.0% |
| ! | ATTCTTTATTAGTGTTTGGA+TGG | - | chr2.3:806871-806890 | MS.gene071411:intron | 25.0% |
| ! | CACATTTATTTAATTGAGTC+GGG | + | chr2.3:806486-806505 | None:intergenic | 25.0% |
| ! | TAGTATATCAAACTCAACAT+AGG | + | chr2.3:807310-807329 | None:intergenic | 25.0% |
| ! | TCAACTAAGTCCTTTAATTA+GGG | - | chr2.3:807021-807040 | MS.gene071411:intron | 25.0% |
| ! | TGTACTAAATGCAGATAAAA+AGG | + | chr2.3:806781-806800 | None:intergenic | 25.0% |
| ! | TTCAACTAAGTCCTTTAATT+AGG | - | chr2.3:807020-807039 | MS.gene071411:intron | 25.0% |
| !! | AAGAATAAAATCTGAAGCAA+TGG | + | chr2.3:806857-806876 | None:intergenic | 25.0% |
| !! | TACAGAAGCATATAGTTATT+TGG | + | chr2.3:807195-807214 | None:intergenic | 25.0% |
| AACATTAAGCAATCAAAGCA+AGG | + | chr2.3:806598-806617 | None:intergenic | 30.0% | |
| AATCATTTATGTATGTCCTG+TGG | - | chr2.3:806158-806177 | MS.gene071411:intron | 30.0% | |
| AGCAAAGTAAGTTTCCATTA+AGG | - | chr2.3:807257-807276 | MS.gene071411:intron | 30.0% | |
| ATTTGATTCAGTTTCTCAAC+AGG | - | chr2.3:806249-806268 | MS.gene071411:intron | 30.0% | |
| GAAGAAGAAAAAGATAAGGA+AGG | + | chr2.3:806185-806204 | None:intergenic | 30.0% | |
| GACATTGGATGATTTCATTA+AGG | - | chr2.3:807590-807609 | MS.gene071411:CDS | 30.0% | |
| GCTAACTCAATATGATATTG+AGG | - | chr2.3:806118-806137 | MS.gene071411:CDS | 30.0% | |
| GTATATGATTCAGATCGTAA+TGG | - | chr2.3:807087-807106 | MS.gene071411:intron | 30.0% | |
| TAACAATTGATGCACCTTAA+TGG | + | chr2.3:807274-807293 | None:intergenic | 30.0% | |
| TTAAATTTGTCACAGGTTCT+TGG | - | chr2.3:807780-807799 | MS.gene071411:CDS | 30.0% | |
| ! | AAAACGTACGTTCAACTTTT+TGG | + | chr2.3:806818-806837 | None:intergenic | 30.0% |
| ! | AAATGGTATATTTGGAACAG+TGG | + | chr2.3:806547-806566 | None:intergenic | 30.0% |
| ! | TTACTGTTCTTTGATTTCTC+AGG | - | chr2.3:807515-807534 | MS.gene071411:intron | 30.0% |
| !! | GAAGTTCTAAAAGATTTGTC+TGG | - | chr2.3:807135-807154 | MS.gene071411:CDS | 30.0% |
| AGAAGATGTCCACTATAAGT+AGG | - | chr2.3:806621-806640 | MS.gene071411:intron | 35.0% | |
| CAACTTGACAGAAATGCTAA+AGG | - | chr2.3:806301-806320 | MS.gene071411:intron | 35.0% | |
| GAAGATGTCCACTATAAGTA+GGG | - | chr2.3:806622-806641 | MS.gene071411:intron | 35.0% | |
| GGTGTCTTTCAAAGACATAT+TGG | - | chr2.3:807113-807132 | MS.gene071411:intron | 35.0% | |
| GTTTGTGTACTTATTTGCAG+AGG | - | chr2.3:806712-806731 | MS.gene071411:intron | 35.0% | |
| TCTGCATTTAGTACAAAAGC+TGG | - | chr2.3:806786-806805 | MS.gene071411:CDS | 35.0% | |
| TGATATGATACCTGAGAAAG+TGG | + | chr2.3:806376-806395 | None:intergenic | 35.0% | |
| TGTTATGAATCCACTTTCTC+AGG | - | chr2.3:806363-806382 | MS.gene071411:CDS | 35.0% | |
| TTGATTTCTCAGGAAGTCTT+AGG | - | chr2.3:807525-807544 | MS.gene071411:intron | 35.0% | |
| ! | AAAAGACTGCTACTTGACAT+TGG | - | chr2.3:807575-807594 | MS.gene071411:CDS | 35.0% |
| ! | AGTGGATTCATAACAAACTC+AGG | + | chr2.3:806358-806377 | None:intergenic | 35.0% |
| ! | TGATTCAGATCGTAATGGAA+AGG | - | chr2.3:807092-807111 | MS.gene071411:intron | 35.0% |
| !! | CATCCATTTTTACTCCAGAA+TGG | + | chr2.3:807806-807825 | None:intergenic | 35.0% |
| AAAAGATAAGGAAGGACCAC+AGG | + | chr2.3:806177-806196 | None:intergenic | 40.0% | |
| AGGTTACTCAAGATGGTTGA+TGG | - | chr2.3:806732-806751 | MS.gene071411:intron | 40.0% | |
| CATGTCTGATGATCAAAGAG+AGG | - | chr2.3:807164-807183 | MS.gene071411:CDS | 40.0% | |
| CCAATACAGCACAACTGAAA+AGG | + | chr2.3:807465-807484 | None:intergenic | 40.0% | |
| TATCCTAGCCCTACTTATAG+TGG | + | chr2.3:806633-806652 | None:intergenic | 40.0% | |
| TTTGCAGAGGTTACTCAAGA+TGG | - | chr2.3:806725-806744 | MS.gene071411:intron | 40.0% | |
| ! | CCTTTTCAGTTGTGCTGTAT+TGG | - | chr2.3:807462-807481 | MS.gene071411:intron | 40.0% |
| !! | TGGCCATTCTGGAGTAAAAA+TGG | - | chr2.3:807800-807819 | MS.gene071411:CDS | 40.0% |
| ATCCTGCTTCTTTCAGAACC+TGG | + | chr2.3:807551-807570 | None:intergenic | 45.0% | |
| TGTCCACTATAAGTAGGGCT+AGG | - | chr2.3:806627-806646 | MS.gene071411:intron | 45.0% | |
| ! | CACAACTGAAAAGGTGTGTG+TGG | + | chr2.3:807456-807475 | None:intergenic | 45.0% |
| !!! | TTTATTTTATTTTATGAAAA+TGG | + | chr2.3:806564-806583 | None:intergenic | 5.0% |
| TCACAGGTTCTTGGCCATTC+TGG | - | chr2.3:807789-807808 | MS.gene071411:CDS | 50.0% | |
| TTCTCAGGAAGTCTTAGGCC+AGG | - | chr2.3:807530-807549 | MS.gene071411:intron | 50.0% | |
| !! | GGCCAGGTTCTGAAAGAAGC+AGG | - | chr2.3:807546-807565 | MS.gene071411:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 806098 | 807845 | 806098 | ID=MS.gene071411 |
| chr2.3 | mRNA | 806098 | 807845 | 806098 | ID=MS.gene071411.t1;Parent=MS.gene071411 |
| chr2.3 | exon | 807776 | 807845 | 807776 | ID=MS.gene071411.t1.exon1;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 807776 | 807845 | 807776 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| chr2.3 | exon | 807559 | 807683 | 807559 | ID=MS.gene071411.t1.exon2;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 807559 | 807683 | 807559 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| chr2.3 | exon | 807115 | 807211 | 807115 | ID=MS.gene071411.t1.exon3;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 807115 | 807211 | 807115 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| chr2.3 | exon | 806758 | 806867 | 806758 | ID=MS.gene071411.t1.exon4;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 806758 | 806867 | 806758 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| chr2.3 | exon | 806332 | 806406 | 806332 | ID=MS.gene071411.t1.exon5;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 806332 | 806406 | 806332 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| chr2.3 | exon | 806098 | 806148 | 806098 | ID=MS.gene071411.t1.exon6;Parent=MS.gene071411.t1 |
| chr2.3 | CDS | 806098 | 806148 | 806098 | ID=cds.MS.gene071411.t1;Parent=MS.gene071411.t1 |
| Gene Sequence |
| Protein sequence |
