Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071414.t1 | XP_003597930.2 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 3.40E-93 | 350.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071414.t1 | P42322 | 37.8 | 172 | 100 | 2 | 1 | 167 | 1 | 170 | 1.6e-27 | 124.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071414.t1 | B7FH75 | 99.4 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 2.5e-93 | 350.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene83474 | MS.gene071414 | PPI |
| MS.gene37035 | MS.gene071414 | PPI |
| MS.gene028111 | MS.gene071414 | PPI |
| MS.gene020092 | MS.gene071414 | PPI |
| MS.gene72809 | MS.gene071414 | PPI |
| MS.gene022826 | MS.gene071414 | PPI |
| MS.gene82121 | MS.gene071414 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071414.t1 | MTR_2g104130 | 99.429 | 175 | 1 | 0 | 1 | 175 | 1 | 175 | 5.87e-128 | 356 |
| MS.gene071414.t1 | MTR_4g130410 | 70.286 | 175 | 52 | 0 | 1 | 175 | 1 | 175 | 1.37e-91 | 264 |
| MS.gene071414.t1 | MTR_4g130410 | 71.429 | 161 | 46 | 0 | 1 | 161 | 1 | 161 | 5.36e-84 | 245 |
| MS.gene071414.t1 | MTR_1g076650 | 27.517 | 149 | 94 | 5 | 22 | 161 | 3 | 146 | 1.56e-11 | 59.7 |
| MS.gene071414.t1 | MTR_1g071150 | 27.517 | 149 | 95 | 5 | 21 | 161 | 2 | 145 | 2.35e-11 | 58.9 |
| MS.gene071414.t1 | MTR_1g041285 | 26.797 | 153 | 98 | 5 | 22 | 165 | 3 | 150 | 2.44e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071414.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071414.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071414.t1 | AT3G18430 | 78.857 | 175 | 37 | 0 | 1 | 175 | 1 | 175 | 1.01e-101 | 290 |
| MS.gene071414.t1 | AT3G22930 | 27.152 | 151 | 96 | 5 | 24 | 165 | 28 | 173 | 5.16e-12 | 61.6 |
Find 23 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCACAGGTTCTTGGCCATTC+TGG | 0.182957 | 2.3:+802541 | MS.gene071414:intron |
| TGTACTAAATGCAGATAAAA+AGG | 0.239607 | 2.3:-801534 | None:intergenic |
| GACATTGGATGATTTCATTA+AGG | 0.296420 | 2.3:+802344 | MS.gene071414:CDS |
| TTAAATTTGTCACAGGTTCT+TGG | 0.342309 | 2.3:+802532 | MS.gene071414:intron |
| TTGATTTCTCAGGAAGTCTT+AGG | 0.365914 | 2.3:+802279 | MS.gene071414:intron |
| AGGTTACTCAAGATGGTTGA+TGG | 0.393812 | 2.3:+801488 | MS.gene071414:CDS |
| TGTTATGAATCCACTTTCTC+AGG | 0.398536 | 2.3:+801120 | MS.gene071414:CDS |
| GGTGTCTTTCAAAGACATAT+TGG | 0.407546 | 2.3:+801869 | MS.gene071414:CDS |
| ATTTGATTCAGTTTCTCAAC+AGG | 0.411656 | 2.3:+801006 | MS.gene071414:intron |
| GAAGTTCTAAAAGATTTGTC+TGG | 0.444669 | 2.3:+801891 | MS.gene071414:CDS |
| ATCCTGCTTCTTTCAGAACC+TGG | 0.474604 | 2.3:-802302 | None:intergenic |
| CAACTTGACAGAAATGCTAA+AGG | 0.478282 | 2.3:+801058 | MS.gene071414:CDS |
| TTTGCAGAGGTTACTCAAGA+TGG | 0.478552 | 2.3:+801481 | MS.gene071414:intron |
| TGATATGATACCTGAGAAAG+TGG | 0.501757 | 2.3:-801130 | None:intergenic |
| TCTGCATTTAGTACAAAAGC+TGG | 0.505330 | 2.3:+801542 | MS.gene071414:CDS |
| GCTAACTCAATATGATATTG+AGG | 0.520808 | 2.3:+800876 | MS.gene071414:CDS |
| TTCTCAGGAAGTCTTAGGCC+AGG | 0.525529 | 2.3:+802284 | MS.gene071414:intron |
| GGCCAGGTTCTGAAAGAAGC+AGG | 0.530644 | 2.3:+802300 | MS.gene071414:CDS |
| GTATATGATTCAGATCGTAA+TGG | 0.556045 | 2.3:+801843 | MS.gene071414:CDS |
| AAAAGACTGCTACTTGACAT+TGG | 0.563411 | 2.3:+802329 | MS.gene071414:CDS |
| AGTGGATTCATAACAAACTC+AGG | 0.575670 | 2.3:-801112 | None:intergenic |
| TGATTCAGATCGTAATGGAA+AGG | 0.613377 | 2.3:+801848 | MS.gene071414:CDS |
| CATGTCTGATGATCAAAGAG+AGG | 0.654475 | 2.3:+801920 | MS.gene071414:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATTATCTAAATTATTT+AGG | - | chr2.3:801675-801694 | None:intergenic | 10.0% |
| !! | TTCTTCAAAAAAAAAAAAAA+AGG | - | chr2.3:802405-802424 | None:intergenic | 10.0% |
| !!! | ATTTTCTTATATATCTCTTT+TGG | + | chr2.3:802374-802393 | MS.gene071414:intron | 15.0% |
| !!! | TTTTAATTTAAATTTGTCAC+AGG | + | chr2.3:802525-802544 | MS.gene071414:intron | 15.0% |
| !!! | TTTTATGAAAATGGTATATT+TGG | - | chr2.3:801312-801331 | None:intergenic | 15.0% |
| !!! | TTTTATTCTTTATTAGTGTT+TGG | + | chr2.3:801623-801642 | MS.gene071414:intron | 15.0% |
| !! | ACACATTTATTTAATTGAGT+CGG | - | chr2.3:801244-801263 | None:intergenic | 20.0% |
| !! | ACTCAATTAAATAAATGTGT+TGG | + | chr2.3:801244-801263 | MS.gene071414:intron | 20.0% |
| !! | ATCATAAATTAGAAGATTTC+AGG | + | chr2.3:801689-801708 | MS.gene071414:intron | 20.0% |
| !! | GAAATGAAATATTCGTATAT+AGG | - | chr2.3:802157-802176 | None:intergenic | 20.0% |
| !!! | ATGTGTTTACTATTTTATAC+AGG | + | chr2.3:801413-801432 | MS.gene071414:intron | 20.0% |
| !!! | GTTCTTTCTAATTGTAATTT+TGG | + | chr2.3:802480-802499 | MS.gene071414:intron | 20.0% |
| !!! | TGTGGAAAAAAGTTTTTTAT+TGG | - | chr2.3:802192-802211 | None:intergenic | 20.0% |
| !!! | TTTGAATTTTAAAGACTTTG+TGG | + | chr2.3:801511-801530 | MS.gene071414:CDS | 20.0% |
| !!! | TTTTTTTTGAAGAAACTCTT+TGG | + | chr2.3:802411-802430 | MS.gene071414:intron | 20.0% |
| ! | ACTATAAGTACCCTAATTAA+AGG | - | chr2.3:801790-801809 | None:intergenic | 25.0% |
| ! | AGAAGAAGAAGAAAAAGATA+AGG | - | chr2.3:800946-800965 | None:intergenic | 25.0% |
| ! | AGTATATCAAACTCAACATA+GGG | - | chr2.3:802066-802085 | None:intergenic | 25.0% |
| ! | ATTCTTTATTAGTGTTTGGA+TGG | + | chr2.3:801627-801646 | MS.gene071414:intron | 25.0% |
| ! | CACATTTATTTAATTGAGTC+GGG | - | chr2.3:801243-801262 | None:intergenic | 25.0% |
| ! | TAGTATATCAAACTCAACAT+AGG | - | chr2.3:802067-802086 | None:intergenic | 25.0% |
| ! | TCAACTAAGTCCTTTAATTA+GGG | + | chr2.3:801777-801796 | MS.gene071414:intron | 25.0% |
| ! | TGTACTAAATGCAGATAAAA+AGG | - | chr2.3:801537-801556 | None:intergenic | 25.0% |
| ! | TTCAACTAAGTCCTTTAATT+AGG | + | chr2.3:801776-801795 | MS.gene071414:intron | 25.0% |
| !! | AAGAATAAAATCTGAAGCAA+TGG | - | chr2.3:801613-801632 | None:intergenic | 25.0% |
| !! | TACAGAAGCATATAGTTATT+AGG | - | chr2.3:801951-801970 | None:intergenic | 25.0% |
| AACATTAAGCAATCAAAGCA+AGG | - | chr2.3:801355-801374 | None:intergenic | 30.0% | |
| AATCATTTATGTATGTCCTG+TGG | + | chr2.3:800916-800935 | MS.gene071414:intron | 30.0% | |
| AGCAAAGTAAGTTTCCATTA+AGG | + | chr2.3:802013-802032 | MS.gene071414:intron | 30.0% | |
| ATTTGATTCAGTTTCTCAAC+AGG | + | chr2.3:801006-801025 | MS.gene071414:intron | 30.0% | |
| GACATTGGATGATTTCATTA+AGG | + | chr2.3:802344-802363 | MS.gene071414:CDS | 30.0% | |
| GCTAACTCAATATGATATTG+AGG | + | chr2.3:800876-800895 | MS.gene071414:CDS | 30.0% | |
| GTATATGATTCAGATCGTAA+TGG | + | chr2.3:801843-801862 | MS.gene071414:CDS | 30.0% | |
| TAACAATTGATGCACCTTAA+TGG | - | chr2.3:802030-802049 | None:intergenic | 30.0% | |
| TTAAATTTGTCACAGGTTCT+TGG | + | chr2.3:802532-802551 | MS.gene071414:intron | 30.0% | |
| ! | AAAACGTACGTTCAACTTTT+TGG | - | chr2.3:801574-801593 | None:intergenic | 30.0% |
| ! | AAATGGTATATTTGGAACAG+TGG | - | chr2.3:801304-801323 | None:intergenic | 30.0% |
| ! | TTACTGTTCTTTGATTTCTC+AGG | + | chr2.3:802269-802288 | MS.gene071414:intron | 30.0% |
| !! | GAAGTTCTAAAAGATTTGTC+TGG | + | chr2.3:801891-801910 | MS.gene071414:CDS | 30.0% |
| AAAAAGATAAGGAAGACCAC+AGG | - | chr2.3:800935-800954 | None:intergenic | 35.0% | |
| AGAAGATGTCCACTATAAGT+AGG | + | chr2.3:801378-801397 | MS.gene071414:intron | 35.0% | |
| CAACTTGACAGAAATGCTAA+AGG | + | chr2.3:801058-801077 | MS.gene071414:CDS | 35.0% | |
| GAAGATGTCCACTATAAGTA+GGG | + | chr2.3:801379-801398 | MS.gene071414:intron | 35.0% | |
| GGTGTCTTTCAAAGACATAT+TGG | + | chr2.3:801869-801888 | MS.gene071414:CDS | 35.0% | |
| GTTTGTGTACTTATTTGCAG+AGG | + | chr2.3:801468-801487 | MS.gene071414:intron | 35.0% | |
| TCTGCATTTAGTACAAAAGC+TGG | + | chr2.3:801542-801561 | MS.gene071414:CDS | 35.0% | |
| TGATATGATACCTGAGAAAG+TGG | - | chr2.3:801133-801152 | None:intergenic | 35.0% | |
| TGTTATGAATCCACTTTCTC+AGG | + | chr2.3:801120-801139 | MS.gene071414:CDS | 35.0% | |
| TTGATTTCTCAGGAAGTCTT+AGG | + | chr2.3:802279-802298 | MS.gene071414:intron | 35.0% | |
| ! | AAAAGACTGCTACTTGACAT+TGG | + | chr2.3:802329-802348 | MS.gene071414:CDS | 35.0% |
| ! | AGTGGATTCATAACAAACTC+AGG | - | chr2.3:801115-801134 | None:intergenic | 35.0% |
| ! | TGATTCAGATCGTAATGGAA+AGG | + | chr2.3:801848-801867 | MS.gene071414:CDS | 35.0% |
| !! | CATCCATTTTTACTCCAGAA+TGG | - | chr2.3:802558-802577 | None:intergenic | 35.0% |
| AGGTTACTCAAGATGGTTGA+TGG | + | chr2.3:801488-801507 | MS.gene071414:CDS | 40.0% | |
| CATGTCTGATGATCAAAGAG+AGG | + | chr2.3:801920-801939 | MS.gene071414:CDS | 40.0% | |
| CCAATACAGCACAACTGAAA+AGG | - | chr2.3:802219-802238 | None:intergenic | 40.0% | |
| TATCCTAGCCCTACTTATAG+TGG | - | chr2.3:801390-801409 | None:intergenic | 40.0% | |
| TCAAACTCAACATAGGGATG+TGG | - | chr2.3:802060-802079 | None:intergenic | 40.0% | |
| TTTGCAGAGGTTACTCAAGA+TGG | + | chr2.3:801481-801500 | MS.gene071414:intron | 40.0% | |
| ! | CCTTTTCAGTTGTGCTGTAT+TGG | + | chr2.3:802216-802235 | MS.gene071414:intron | 40.0% |
| !! | TGGCCATTCTGGAGTAAAAA+TGG | + | chr2.3:802552-802571 | MS.gene071414:CDS | 40.0% |
| ATCCTGCTTCTTTCAGAACC+TGG | - | chr2.3:802305-802324 | None:intergenic | 45.0% | |
| TGTCCACTATAAGTAGGGCT+AGG | + | chr2.3:801384-801403 | MS.gene071414:intron | 45.0% | |
| ! | CACAACTGAAAAGGTGTGTG+TGG | - | chr2.3:802210-802229 | None:intergenic | 45.0% |
| !!! | TTTATTTTATTTTATGAAAA+TGG | - | chr2.3:801321-801340 | None:intergenic | 5.0% |
| TCACAGGTTCTTGGCCATTC+TGG | + | chr2.3:802541-802560 | MS.gene071414:intron | 50.0% | |
| TTCTCAGGAAGTCTTAGGCC+AGG | + | chr2.3:802284-802303 | MS.gene071414:intron | 50.0% | |
| !! | GGCCAGGTTCTGAAAGAAGC+AGG | + | chr2.3:802300-802319 | MS.gene071414:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 800856 | 802597 | 800856 | ID=MS.gene071414 |
| chr2.3 | mRNA | 800856 | 802597 | 800856 | ID=MS.gene071414.t1;Parent=MS.gene071414 |
| chr2.3 | exon | 800856 | 800925 | 800856 | ID=MS.gene071414.t1.exon1;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 800856 | 800925 | 800856 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| chr2.3 | exon | 801017 | 801141 | 801017 | ID=MS.gene071414.t1.exon2;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 801017 | 801141 | 801017 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| chr2.3 | exon | 801488 | 801584 | 801488 | ID=MS.gene071414.t1.exon3;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 801488 | 801584 | 801488 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| chr2.3 | exon | 801832 | 801941 | 801832 | ID=MS.gene071414.t1.exon4;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 801832 | 801941 | 801832 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| chr2.3 | exon | 802291 | 802365 | 802291 | ID=MS.gene071414.t1.exon5;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 802291 | 802365 | 802291 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| chr2.3 | exon | 802547 | 802597 | 802547 | ID=MS.gene071414.t1.exon6;Parent=MS.gene071414.t1 |
| chr2.3 | CDS | 802547 | 802597 | 802547 | ID=cds.MS.gene071414.t1;Parent=MS.gene071414.t1 |
| Gene Sequence |
| Protein sequence |
