Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072699.t1 | XP_003625969.1 | 96.1 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 2.30E-59 | 238 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072699.t1 | Q9LZP9 | 59.4 | 133 | 46 | 5 | 1 | 127 | 1 | 131 | 1.1e-30 | 134.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072699.t1 | G7KST6 | 96.1 | 127 | 4 | 1 | 1 | 126 | 1 | 127 | 1.7e-59 | 238.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055277 | MS.gene072699 | 0.802777 | 4.78E-49 | -1.69E-46 |
| MS.gene057554 | MS.gene072699 | 0.817649 | 2.95E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene072699 | 0.801177 | 1.02E-48 | -1.69E-46 |
| MS.gene059894 | MS.gene072699 | 0.807224 | 5.61E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene072699 | 0.800476 | 1.42E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072699.t1 | MTR_7g109520 | 95.312 | 128 | 5 | 1 | 1 | 127 | 1 | 128 | 3.79e-87 | 249 |
| MS.gene072699.t1 | MTR_1g087030 | 64.615 | 130 | 41 | 3 | 2 | 127 | 3 | 131 | 5.61e-54 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene072699.t1 | AT3G62410 | 59.398 | 133 | 46 | 5 | 1 | 127 | 1 | 131 | 4.33e-45 | 143 |
| MS.gene072699.t1 | AT2G47400 | 68.041 | 97 | 27 | 2 | 33 | 127 | 30 | 124 | 4.23e-40 | 130 |
| MS.gene072699.t1 | AT1G76560 | 47.297 | 74 | 37 | 1 | 56 | 127 | 61 | 134 | 5.85e-19 | 77.0 |
Find 41 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTCTTTGATATCTGTTC+TGG | 0.263996 | 7.3:-5457191 | None:intergenic |
| TGTTGTTGGTGAGATCTTGA+TGG | 0.363034 | 7.3:-5457099 | None:intergenic |
| AAGCTTCTCTTCAACTCATC+AGG | 0.395970 | 7.3:+5457065 | MS.gene072699:CDS |
| GCTACGCATTCTCCACTAAC+CGG | 0.412486 | 7.3:-5457257 | None:intergenic |
| GAAGAAGAAATGGCAACAAT+AGG | 0.418272 | 7.3:+5457020 | None:intergenic |
| GATGAGTTGAAGAGAAGCTT+GGG | 0.422285 | 7.3:-5457062 | None:intergenic |
| CACCATCATTCGACCTGATC+CGG | 0.449625 | 7.3:-5457147 | None:intergenic |
| CGGTGGGGTGTAGCCGGATC+AGG | 0.450264 | 7.3:+5457134 | MS.gene072699:CDS |
| CGTGTCTGCAGTGGTGTTGT+TGG | 0.454736 | 7.3:-5457113 | None:intergenic |
| GGTGAGATCTTGATGGTTTG+TGG | 0.456742 | 7.3:-5457092 | None:intergenic |
| TGTTCTGGTGCAGCATGAAC+TGG | 0.466575 | 7.3:-5457176 | None:intergenic |
| TGGAGAATGCGTAGCGGCAT+GGG | 0.484341 | 7.3:+5457265 | MS.gene072699:CDS |
| TGTGCTGATGACCCGGTTAG+TGG | 0.494167 | 7.3:+5457245 | MS.gene072699:CDS |
| GAAGAAGGATTCTGATCCAT+TGG | 0.495582 | 7.3:+5457334 | MS.gene072699:CDS |
| CGGCGAGTCATGCAAGGGAT+AGG | 0.497910 | 7.3:+5457312 | MS.gene072699:CDS |
| GTGGAGAATGCGTAGCGGCA+TGG | 0.516210 | 7.3:+5457264 | MS.gene072699:CDS |
| CAGACACGGTGGGGTGTAGC+CGG | 0.517241 | 7.3:+5457128 | MS.gene072699:CDS |
| TGATGAGTTGAAGAGAAGCT+TGG | 0.520626 | 7.3:-5457063 | None:intergenic |
| AGAGAAGCTTGGGGTTTGAA+AGG | 0.525586 | 7.3:-5457052 | None:intergenic |
| TCCATTGGAGGATTACTGTA+AGG | 0.532128 | 7.3:+5457349 | MS.gene072699:CDS |
| GTGCTGCGGCGAGTCATGCA+AGG | 0.540725 | 7.3:+5457306 | MS.gene072699:CDS |
| TCATGCAAGGGATAGGAAGA+AGG | 0.572718 | 7.3:+5457319 | MS.gene072699:CDS |
| ACCAGAACAGATATCAAAGA+AGG | 0.574036 | 7.3:+5457190 | MS.gene072699:CDS |
| AGAGACATGTGCTGATGACC+CGG | 0.581174 | 7.3:+5457238 | MS.gene072699:CDS |
| TCCTTACAGTAATCCTCCAA+TGG | 0.581674 | 7.3:-5457350 | None:intergenic |
| AGCGGCATGGGACGAAGTGG+AGG | 0.586526 | 7.3:+5457277 | MS.gene072699:CDS |
| AGCCGGATCAGGTCGAATGA+TGG | 0.587328 | 7.3:+5457145 | MS.gene072699:CDS |
| CTACGCATTCTCCACTAACC+GGG | 0.589171 | 7.3:-5457256 | None:intergenic |
| CAACAACACCACTGCAGACA+CGG | 0.593172 | 7.3:+5457114 | MS.gene072699:CDS |
| GAAGGATTCTGATCCATTGG+AGG | 0.594880 | 7.3:+5457337 | MS.gene072699:CDS |
| GAAGAAATGGCAACAATAGG+TGG | 0.596013 | 7.3:+5457023 | None:intergenic |
| ACACCCCACCGTGTCTGCAG+TGG | 0.598200 | 7.3:-5457122 | None:intergenic |
| AACACCACTGCAGACACGGT+GGG | 0.598529 | 7.3:+5457118 | MS.gene072699:CDS |
| CGTAGCGGCATGGGACGAAG+TGG | 0.601693 | 7.3:+5457274 | MS.gene072699:CDS |
| ACACCACTGCAGACACGGTG+GGG | 0.612423 | 7.3:+5457119 | MS.gene072699:CDS |
| CGGATCAGGTCGAATGATGG+TGG | 0.612425 | 7.3:+5457148 | MS.gene072699:CDS |
| TGCTGCGGCGAGTCATGCAA+GGG | 0.625380 | 7.3:+5457307 | MS.gene072699:CDS |
| CAACACCACTGCAGACACGG+TGG | 0.673443 | 7.3:+5457117 | MS.gene072699:CDS |
| ATGAGTTGAAGAGAAGCTTG+GGG | 0.682917 | 7.3:-5457061 | None:intergenic |
| AGTGGAGGAACTCAGTGCTG+CGG | 0.683881 | 7.3:+5457292 | MS.gene072699:CDS |
| GGTTAGTGGAGAATGCGTAG+CGG | 0.759059 | 7.3:+5457259 | MS.gene072699:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCAGAACAGATATCAAAGA+AGG | + | chr7.3:5457190-5457209 | MS.gene072699:CDS | 35.0% | |
| ACCTTCTTTGATATCTGTTC+TGG | - | chr7.3:5457194-5457213 | None:intergenic | 35.0% | |
| AAGCTTCTCTTCAACTCATC+AGG | + | chr7.3:5457065-5457084 | MS.gene072699:CDS | 40.0% | |
| GAAGAAGGATTCTGATCCAT+TGG | + | chr7.3:5457334-5457353 | MS.gene072699:CDS | 40.0% | |
| TCCATTGGAGGATTACTGTA+AGG | + | chr7.3:5457349-5457368 | MS.gene072699:CDS | 40.0% | |
| TCCTTACAGTAATCCTCCAA+TGG | - | chr7.3:5457353-5457372 | None:intergenic | 40.0% | |
| ! | ATGAGTTGAAGAGAAGCTTG+GGG | - | chr7.3:5457064-5457083 | None:intergenic | 40.0% |
| ! | GATGAGTTGAAGAGAAGCTT+GGG | - | chr7.3:5457065-5457084 | None:intergenic | 40.0% |
| ! | TGATGAGTTGAAGAGAAGCT+TGG | - | chr7.3:5457066-5457085 | None:intergenic | 40.0% |
| ! | TGTTGTTGGTGAGATCTTGA+TGG | - | chr7.3:5457102-5457121 | None:intergenic | 40.0% |
| ! | TTTTGCACTCATCAGTCTCA+GGG | - | chr7.3:5457379-5457398 | None:intergenic | 40.0% |
| AGAGAAGCTTGGGGTTTGAA+AGG | - | chr7.3:5457055-5457074 | None:intergenic | 45.0% | |
| GAAGGATTCTGATCCATTGG+AGG | + | chr7.3:5457337-5457356 | MS.gene072699:CDS | 45.0% | |
| TCATGCAAGGGATAGGAAGA+AGG | + | chr7.3:5457319-5457338 | MS.gene072699:CDS | 45.0% | |
| ! | GGTGAGATCTTGATGGTTTG+TGG | - | chr7.3:5457095-5457114 | None:intergenic | 45.0% |
| ! | GTTTTGCACTCATCAGTCTC+AGG | - | chr7.3:5457380-5457399 | None:intergenic | 45.0% |
| CAACAACACCACTGCAGACA+CGG | + | chr7.3:5457114-5457133 | MS.gene072699:CDS | 50.0% | |
| CACCATCATTCGACCTGATC+CGG | - | chr7.3:5457150-5457169 | None:intergenic | 50.0% | |
| CTACGCATTCTCCACTAACC+GGG | - | chr7.3:5457259-5457278 | None:intergenic | 50.0% | |
| GCTACGCATTCTCCACTAAC+CGG | - | chr7.3:5457260-5457279 | None:intergenic | 50.0% | |
| GGTTAGTGGAGAATGCGTAG+CGG | + | chr7.3:5457259-5457278 | MS.gene072699:CDS | 50.0% | |
| TGTTCTGGTGCAGCATGAAC+TGG | - | chr7.3:5457179-5457198 | None:intergenic | 50.0% | |
| ! | AGAGACATGTGCTGATGACC+CGG | + | chr7.3:5457238-5457257 | MS.gene072699:CDS | 50.0% |
| AACACCACTGCAGACACGGT+GGG | + | chr7.3:5457118-5457137 | MS.gene072699:CDS | 55.0% | |
| AGCCGGATCAGGTCGAATGA+TGG | + | chr7.3:5457145-5457164 | MS.gene072699:CDS | 55.0% | |
| AGTGGAGGAACTCAGTGCTG+CGG | + | chr7.3:5457292-5457311 | MS.gene072699:CDS | 55.0% | |
| CGGATCAGGTCGAATGATGG+TGG | + | chr7.3:5457148-5457167 | MS.gene072699:CDS | 55.0% | |
| TGGAGAATGCGTAGCGGCAT+GGG | + | chr7.3:5457265-5457284 | MS.gene072699:CDS | 55.0% | |
| ! | TGTGCTGATGACCCGGTTAG+TGG | + | chr7.3:5457245-5457264 | MS.gene072699:CDS | 55.0% |
| !! | CGTGTCTGCAGTGGTGTTGT+TGG | - | chr7.3:5457116-5457135 | None:intergenic | 55.0% |
| ACACCACTGCAGACACGGTG+GGG | + | chr7.3:5457119-5457138 | MS.gene072699:CDS | 60.0% | |
| CAACACCACTGCAGACACGG+TGG | + | chr7.3:5457117-5457136 | MS.gene072699:CDS | 60.0% | |
| CGGCGAGTCATGCAAGGGAT+AGG | + | chr7.3:5457312-5457331 | MS.gene072699:CDS | 60.0% | |
| GTGGAGAATGCGTAGCGGCA+TGG | + | chr7.3:5457264-5457283 | MS.gene072699:CDS | 60.0% | |
| TGCTGCGGCGAGTCATGCAA+GGG | + | chr7.3:5457307-5457326 | MS.gene072699:CDS | 60.0% | |
| ACACCCCACCGTGTCTGCAG+TGG | - | chr7.3:5457125-5457144 | None:intergenic | 65.0% | |
| AGCGGCATGGGACGAAGTGG+AGG | + | chr7.3:5457277-5457296 | MS.gene072699:CDS | 65.0% | |
| CAGACACGGTGGGGTGTAGC+CGG | + | chr7.3:5457128-5457147 | MS.gene072699:CDS | 65.0% | |
| CGTAGCGGCATGGGACGAAG+TGG | + | chr7.3:5457274-5457293 | MS.gene072699:CDS | 65.0% | |
| GTGCTGCGGCGAGTCATGCA+AGG | + | chr7.3:5457306-5457325 | MS.gene072699:CDS | 65.0% | |
| CGGTGGGGTGTAGCCGGATC+AGG | + | chr7.3:5457134-5457153 | MS.gene072699:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 5457029 | 5457412 | 5457029 | ID=MS.gene072699 |
| chr7.3 | mRNA | 5457029 | 5457412 | 5457029 | ID=MS.gene072699.t1;Parent=MS.gene072699 |
| chr7.3 | exon | 5457029 | 5457412 | 5457029 | ID=MS.gene072699.t1.exon1;Parent=MS.gene072699.t1 |
| chr7.3 | CDS | 5457029 | 5457412 | 5457029 | ID=cds.MS.gene072699.t1;Parent=MS.gene072699.t1 |
| Gene Sequence |
| Protein sequence |
