Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07275.t1 | XP_003591419.1 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 1.50E-102 | 382.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07275.t1 | G7IDR9 | 96.4 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 1.1e-102 | 382.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene07275 | 0.82134 | 4.25E-53 | -1.69E-46 |
| MS.gene051757 | MS.gene07275 | 0.803653 | 3.15E-49 | -1.69E-46 |
| MS.gene053381 | MS.gene07275 | 0.817074 | 3.98E-52 | -1.69E-46 |
| MS.gene055311 | MS.gene07275 | 0.80987 | 1.53E-50 | -1.69E-46 |
| MS.gene055313 | MS.gene07275 | 0.802008 | 6.88E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene07275 | 0.820373 | 7.10E-53 | -1.69E-46 |
| MS.gene060870 | MS.gene07275 | 0.828272 | 9.86E-55 | -1.69E-46 |
| MS.gene060998 | MS.gene07275 | 0.80976 | 1.61E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07275.t1 | MTR_1g087230 | 96.354 | 192 | 7 | 0 | 1 | 192 | 1 | 192 | 8.02e-139 | 385 |
| MS.gene07275.t1 | MTR_7g109410 | 56.345 | 197 | 76 | 5 | 1 | 192 | 1 | 192 | 1.17e-72 | 218 |
| MS.gene07275.t1 | MTR_8g107420 | 27.841 | 176 | 112 | 4 | 1 | 176 | 1 | 161 | 5.77e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07275.t1 | AT4G02090 | 37.273 | 220 | 88 | 10 | 1 | 189 | 1 | 201 | 5.43e-32 | 114 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATTTGAATAGGCAGATTTC+AGG | 0.248523 | 1.4:+69518088 | MS.gene07275:CDS |
| GCCTAGCTTGGATACTATTA+AGG | 0.311308 | 1.4:+69518123 | MS.gene07275:CDS |
| GGTAAAGTTGTATTCTTAGA+TGG | 0.327818 | 1.4:+69517659 | MS.gene07275:CDS |
| GTTCTTATAAGTTTCTTCCT+TGG | 0.362756 | 1.4:+69517939 | MS.gene07275:CDS |
| ATCTGCCTATTCAAATAATC+TGG | 0.371640 | 1.4:-69518082 | None:intergenic |
| GGAAGAGGTGATGGAGGAGA+AGG | 0.379095 | 1.4:+69518009 | MS.gene07275:CDS |
| TCTTCGCCGCTTAAACCAAT+AGG | 0.386729 | 1.4:-69517860 | None:intergenic |
| GGAGAATTTGCCGGACATAT+TGG | 0.397303 | 1.4:+69518051 | MS.gene07275:CDS |
| ATAGGTTTCCCTTGCTTCAC+TGG | 0.408758 | 1.4:-69517842 | None:intergenic |
| TTTCGTCCTTCAGATTCAAC+AGG | 0.424376 | 1.4:+69517638 | MS.gene07275:CDS |
| ATCTCTAGCTTCTTGTCAGC+AGG | 0.426386 | 1.4:-69517797 | None:intergenic |
| GTGAAGCAAGGGAAACCTAT+TGG | 0.427138 | 1.4:+69517845 | MS.gene07275:CDS |
| GGAGGCCAGATTATTTGAAT+AGG | 0.439315 | 1.4:+69518077 | MS.gene07275:CDS |
| AGCTTCTTGTCAGCAGGTAA+TGG | 0.466025 | 1.4:-69517791 | None:intergenic |
| GAAGAGGTGATGGAGGAGAA+GGG | 0.490391 | 1.4:+69518010 | MS.gene07275:CDS |
| GAAAGGGTGGAAGCCTAGCT+TGG | 0.506451 | 1.4:+69518111 | MS.gene07275:CDS |
| TCGGAGGAAGGAAGAGGTGA+TGG | 0.513945 | 1.4:+69518000 | MS.gene07275:CDS |
| ATGTCATAACAGTGAAATAG+TGG | 0.517051 | 1.4:+69517973 | MS.gene07275:CDS |
| TTGTCAGCAGGTAATGGTGT+TGG | 0.520247 | 1.4:-69517785 | None:intergenic |
| ATTTGAATAGGCAGATTTCA+GGG | 0.521145 | 1.4:+69518089 | MS.gene07275:CDS |
| AATAGGCAGATTTCAGGGAA+AGG | 0.530769 | 1.4:+69518094 | MS.gene07275:CDS |
| GGCCTCCCTTCCAATATGTC+CGG | 0.533242 | 1.4:-69518061 | None:intergenic |
| TGATGCTTCCAGTGAAGCAA+GGG | 0.535617 | 1.4:+69517834 | MS.gene07275:CDS |
| AACAGTGGCAGAGTTAATGA+TGG | 0.548929 | 1.4:+69517709 | MS.gene07275:CDS |
| ATAGGCAGATTTCAGGGAAA+GGG | 0.549284 | 1.4:+69518095 | MS.gene07275:CDS |
| GGAGCCTCGGAGGAAGGAAG+AGG | 0.553960 | 1.4:+69517994 | MS.gene07275:CDS |
| TTGATGCTTCCAGTGAAGCA+AGG | 0.559013 | 1.4:+69517833 | MS.gene07275:CDS |
| ACTTTACCTGTTGAATCTGA+AGG | 0.560942 | 1.4:-69517644 | None:intergenic |
| AATTTGCCGGACATATTGGA+AGG | 0.563693 | 1.4:+69518055 | MS.gene07275:CDS |
| TCCTTAATAGTATCCAAGCT+AGG | 0.569966 | 1.4:-69518124 | None:intergenic |
| AATAGTGGAGCCTCGGAGGA+AGG | 0.571841 | 1.4:+69517988 | MS.gene07275:CDS |
| GGGAAACCTATTGGTTTAAG+CGG | 0.579272 | 1.4:+69517854 | MS.gene07275:CDS |
| ACAGTGAAATAGTGGAGCCT+CGG | 0.582711 | 1.4:+69517981 | MS.gene07275:CDS |
| GAGGAAGGAAGAGGTGATGG+AGG | 0.589938 | 1.4:+69518003 | MS.gene07275:CDS |
| GGCAGATTTCAGGGAAAGGG+TGG | 0.595138 | 1.4:+69518098 | MS.gene07275:CDS |
| AATTAACCATCAACAAAACT+CGG | 0.602000 | 1.4:-69517889 | None:intergenic |
| TGCCGGACATATTGGAAGGG+AGG | 0.607569 | 1.4:+69518059 | MS.gene07275:CDS |
| GTGAAATAGTGGAGCCTCGG+AGG | 0.619098 | 1.4:+69517984 | MS.gene07275:CDS |
| ATTTGCCGGACATATTGGAA+GGG | 0.657578 | 1.4:+69518056 | MS.gene07275:CDS |
| ATCACCTCTTCCTTCCTCCG+AGG | 0.665878 | 1.4:-69517998 | None:intergenic |
| TGACATAAACTACTAAACCA+AGG | 0.674454 | 1.4:-69517956 | None:intergenic |
| ATTTGATGAAGCAATAACAG+TGG | 0.710910 | 1.4:+69517694 | MS.gene07275:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTAACCATCAACAAAACT+CGG | - | chr1.4:69517892-69517911 | None:intergenic | 25.0% |
| ! | TTGAGAAAAAACATACTCAT+TGG | + | chr1.4:69518155-69518174 | MS.gene07275:CDS | 25.0% |
| ATCTGCCTATTCAAATAATC+TGG | - | chr1.4:69518085-69518104 | None:intergenic | 30.0% | |
| ATGTCATAACAGTGAAATAG+TGG | + | chr1.4:69517973-69517992 | MS.gene07275:CDS | 30.0% | |
| ATTTGAATAGGCAGATTTCA+GGG | + | chr1.4:69518089-69518108 | MS.gene07275:CDS | 30.0% | |
| ATTTGATGAAGCAATAACAG+TGG | + | chr1.4:69517694-69517713 | MS.gene07275:CDS | 30.0% | |
| GGTAAAGTTGTATTCTTAGA+TGG | + | chr1.4:69517659-69517678 | MS.gene07275:CDS | 30.0% | |
| GTTCTTATAAGTTTCTTCCT+TGG | + | chr1.4:69517939-69517958 | MS.gene07275:CDS | 30.0% | |
| TATTTGAATAGGCAGATTTC+AGG | + | chr1.4:69518088-69518107 | MS.gene07275:CDS | 30.0% | |
| TGACATAAACTACTAAACCA+AGG | - | chr1.4:69517959-69517978 | None:intergenic | 30.0% | |
| ACTTTACCTGTTGAATCTGA+AGG | - | chr1.4:69517647-69517666 | None:intergenic | 35.0% | |
| ATTCAGCTGTGAACCAAAAA+AGG | + | chr1.4:69517762-69517781 | MS.gene07275:CDS | 35.0% | |
| TCCTTAATAGTATCCAAGCT+AGG | - | chr1.4:69518127-69518146 | None:intergenic | 35.0% | |
| !!! | AACTCAACCACTACTTTTTG+TGG | - | chr1.4:69517740-69517759 | None:intergenic | 35.0% |
| AACAGTGGCAGAGTTAATGA+TGG | + | chr1.4:69517709-69517728 | MS.gene07275:CDS | 40.0% | |
| AATAGGCAGATTTCAGGGAA+AGG | + | chr1.4:69518094-69518113 | MS.gene07275:CDS | 40.0% | |
| AATTTGCCGGACATATTGGA+AGG | + | chr1.4:69518055-69518074 | MS.gene07275:CDS | 40.0% | |
| ATAGGCAGATTTCAGGGAAA+GGG | + | chr1.4:69518095-69518114 | MS.gene07275:CDS | 40.0% | |
| ATTTGCCGGACATATTGGAA+GGG | + | chr1.4:69518056-69518075 | MS.gene07275:CDS | 40.0% | |
| GCCTAGCTTGGATACTATTA+AGG | + | chr1.4:69518123-69518142 | MS.gene07275:CDS | 40.0% | |
| GGAGGCCAGATTATTTGAAT+AGG | + | chr1.4:69518077-69518096 | MS.gene07275:CDS | 40.0% | |
| GGATCATCCACAAAAAGTAG+TGG | + | chr1.4:69517730-69517749 | MS.gene07275:CDS | 40.0% | |
| TTTCGTCCTTCAGATTCAAC+AGG | + | chr1.4:69517638-69517657 | MS.gene07275:CDS | 40.0% | |
| ! | GGGAGAGAGATACGATTTTT+CGG | + | chr1.4:69518030-69518049 | MS.gene07275:CDS | 40.0% |
| !! | CGATTTTTCGGAGAATTTGC+CGG | + | chr1.4:69518042-69518061 | MS.gene07275:CDS | 40.0% |
| !! | GGGAAACCTATTGGTTTAAG+CGG | + | chr1.4:69517854-69517873 | MS.gene07275:CDS | 40.0% |
| !!! | GTAATGGTGTTGGCCTTTTT+TGG | - | chr1.4:69517778-69517797 | None:intergenic | 40.0% |
| AGCTTCTTGTCAGCAGGTAA+TGG | - | chr1.4:69517794-69517813 | None:intergenic | 45.0% | |
| ATAGGTTTCCCTTGCTTCAC+TGG | - | chr1.4:69517845-69517864 | None:intergenic | 45.0% | |
| GGAGAATTTGCCGGACATAT+TGG | + | chr1.4:69518051-69518070 | MS.gene07275:CDS | 45.0% | |
| GTGAAGCAAGGGAAACCTAT+TGG | + | chr1.4:69517845-69517864 | MS.gene07275:CDS | 45.0% | |
| TCTTCGCCGCTTAAACCAAT+AGG | - | chr1.4:69517863-69517882 | None:intergenic | 45.0% | |
| TGATGCTTCCAGTGAAGCAA+GGG | + | chr1.4:69517834-69517853 | MS.gene07275:CDS | 45.0% | |
| TTGATGCTTCCAGTGAAGCA+AGG | + | chr1.4:69517833-69517852 | MS.gene07275:CDS | 45.0% | |
| ! | ACAGTGAAATAGTGGAGCCT+CGG | + | chr1.4:69517981-69518000 | MS.gene07275:CDS | 45.0% |
| ! | ATCTCTAGCTTCTTGTCAGC+AGG | - | chr1.4:69517800-69517819 | None:intergenic | 45.0% |
| ! | TTGTCAGCAGGTAATGGTGT+TGG | - | chr1.4:69517788-69517807 | None:intergenic | 45.0% |
| !!! | AACTCGCCGAGTTTTGTTGA+TGG | + | chr1.4:69517883-69517902 | MS.gene07275:CDS | 45.0% |
| !! | GAAGAGGTGATGGAGGAGAA+GGG | + | chr1.4:69518010-69518029 | MS.gene07275:CDS | 50.0% |
| ATCACCTCTTCCTTCCTCCG+AGG | - | chr1.4:69518001-69518020 | None:intergenic | 55.0% | |
| GAAAGGGTGGAAGCCTAGCT+TGG | + | chr1.4:69518111-69518130 | MS.gene07275:CDS | 55.0% | |
| GGCAGATTTCAGGGAAAGGG+TGG | + | chr1.4:69518098-69518117 | MS.gene07275:CDS | 55.0% | |
| GGCCTCCCTTCCAATATGTC+CGG | - | chr1.4:69518064-69518083 | None:intergenic | 55.0% | |
| TCGGAGGAAGGAAGAGGTGA+TGG | + | chr1.4:69518000-69518019 | MS.gene07275:CDS | 55.0% | |
| TGCCGGACATATTGGAAGGG+AGG | + | chr1.4:69518059-69518078 | MS.gene07275:CDS | 55.0% | |
| ! | AATAGTGGAGCCTCGGAGGA+AGG | + | chr1.4:69517988-69518007 | MS.gene07275:CDS | 55.0% |
| ! | GAGGAAGGAAGAGGTGATGG+AGG | + | chr1.4:69518003-69518022 | MS.gene07275:CDS | 55.0% |
| ! | GTGAAATAGTGGAGCCTCGG+AGG | + | chr1.4:69517984-69518003 | MS.gene07275:CDS | 55.0% |
| !! | GGAAGAGGTGATGGAGGAGA+AGG | + | chr1.4:69518009-69518028 | MS.gene07275:CDS | 55.0% |
| GGAGCCTCGGAGGAAGGAAG+AGG | + | chr1.4:69517994-69518013 | MS.gene07275:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69517620 | 69518198 | 69517620 | ID=MS.gene07275 |
| chr1.4 | mRNA | 69517620 | 69518198 | 69517620 | ID=MS.gene07275.t1;Parent=MS.gene07275 |
| chr1.4 | exon | 69517620 | 69518198 | 69517620 | ID=MS.gene07275.t1.exon1;Parent=MS.gene07275.t1 |
| chr1.4 | CDS | 69517620 | 69518198 | 69517620 | ID=cds.MS.gene07275.t1;Parent=MS.gene07275.t1 |
| Gene Sequence |
| Protein sequence |
