Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07287.t1 | XP_003591401.1 | 95.4 | 131 | 6 | 0 | 1 | 131 | 1 | 131 | 1.70E-60 | 241.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07287.t1 | Q9LZP9 | 63.8 | 105 | 34 | 2 | 31 | 131 | 27 | 131 | 1.4e-28 | 127.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07287.t1 | B7FGI5 | 95.4 | 131 | 6 | 0 | 1 | 131 | 1 | 131 | 1.2e-60 | 241.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050404 | MS.gene07287 | 0.817864 | 2.64E-52 | -1.69E-46 |
| MS.gene050406 | MS.gene07287 | 0.815653 | 8.28E-52 | -1.69E-46 |
| MS.gene051171 | MS.gene07287 | 0.809569 | 1.77E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene07287 | 0.828076 | 1.10E-54 | -1.69E-46 |
| MS.gene056633 | MS.gene07287 | 0.814247 | 1.70E-51 | -1.69E-46 |
| MS.gene056664 | MS.gene07287 | 0.80102 | 1.10E-48 | -1.69E-46 |
| MS.gene058746 | MS.gene07287 | 0.822103 | 2.83E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07287.t1 | MTR_1g087030 | 95.420 | 131 | 6 | 0 | 1 | 131 | 1 | 131 | 3.06e-90 | 257 |
| MS.gene07287.t1 | MTR_7g109520 | 59.542 | 131 | 48 | 3 | 1 | 130 | 1 | 127 | 1.95e-47 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07287.t1 | AT3G62410 | 52.593 | 135 | 56 | 3 | 1 | 131 | 1 | 131 | 2.28e-42 | 136 |
| MS.gene07287.t1 | AT2G47400 | 62.745 | 102 | 33 | 4 | 32 | 131 | 26 | 124 | 1.02e-33 | 114 |
| MS.gene07287.t1 | AT1G76560 | 48.611 | 72 | 35 | 1 | 62 | 131 | 63 | 134 | 1.03e-17 | 73.9 |
Find 48 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGCTCTCTTCCACCTTCC+CGG | 0.302904 | 1.4:-69383192 | None:intergenic |
| TGACTGCACCGGTCCATCTT+TGG | 0.344117 | 1.4:-69383118 | None:intergenic |
| CAAGCATCACCGGACATAAC+CGG | 0.376705 | 1.4:+69383173 | MS.gene07287:CDS |
| AAGTCTCAGGCCATCAGGTC+TGG | 0.407675 | 1.4:+69383077 | MS.gene07287:CDS |
| TTCAAAAGTCTCAGGCCATC+AGG | 0.431081 | 1.4:+69383072 | MS.gene07287:CDS |
| TCCACCTTCCCGGTTATGTC+CGG | 0.434096 | 1.4:-69383182 | None:intergenic |
| GGCGGCGTGGGATGAGGTTG+AGG | 0.441926 | 1.4:+69383265 | MS.gene07287:CDS |
| TGGACCGGTGCAGTCAGTTC+CGG | 0.447899 | 1.4:+69383125 | MS.gene07287:CDS |
| AGAGTCTCTTCAAAAGTCTC+AGG | 0.482723 | 1.4:+69383064 | MS.gene07287:CDS |
| TGTTCATGCTCAACCAAAGA+TGG | 0.482933 | 1.4:+69383105 | MS.gene07287:CDS |
| ATGTGTGGCGGCGTGGGATG+AGG | 0.483397 | 1.4:+69383259 | MS.gene07287:CDS |
| CGGTGAATGTGTGGCGGCGT+GGG | 0.493131 | 1.4:+69383253 | MS.gene07287:CDS |
| ATAGTATTATCAGTCTTCAT+AGG | 0.497307 | 1.4:-69383387 | None:intergenic |
| AGTGCAGAAGAGGCGTGTGC+AGG | 0.498117 | 1.4:+69383218 | MS.gene07287:CDS |
| GGAATACTGCAAGGACAATC+CGG | 0.499926 | 1.4:+69383346 | MS.gene07287:CDS |
| GTGCGGCGGCAAGCCATGCA+AGG | 0.505659 | 1.4:+69383294 | MS.gene07287:CDS |
| GGTCCCTTGAAAATAACTCT+AGG | 0.516561 | 1.4:-69383041 | None:intergenic |
| TCGACCGGTGCAAGCATCAC+CGG | 0.518958 | 1.4:+69383163 | MS.gene07287:CDS |
| CGCCGCTAGCGGTGAATGTG+TGG | 0.526479 | 1.4:+69383244 | MS.gene07287:CDS |
| TGCAGTTATGGCAGCAACAA+TGG | 0.530066 | 1.4:+69382998 | None:intergenic |
| TGCAGTCAGTTCCGGTAGGA+TGG | 0.539303 | 1.4:+69383133 | MS.gene07287:CDS |
| GAGCATAAAGAGTGCAGAAG+AGG | 0.542662 | 1.4:+69383208 | MS.gene07287:CDS |
| GTCCCTTGAAAATAACTCTA+GGG | 0.561714 | 1.4:-69383040 | None:intergenic |
| AAGCATCACCGGACATAACC+GGG | 0.568302 | 1.4:+69383174 | MS.gene07287:CDS |
| CGCCACACATTCACCGCTAG+CGG | 0.569137 | 1.4:-69383246 | None:intergenic |
| ATGCTCAACCAAAGATGGAC+CGG | 0.572661 | 1.4:+69383110 | MS.gene07287:CDS |
| TCAGGCCATCAGGTCTGGAC+CGG | 0.575950 | 1.4:+69383082 | MS.gene07287:CDS |
| TGCGGCGGCAAGCCATGCAA+GGG | 0.578805 | 1.4:+69383295 | MS.gene07287:CDS |
| TCCTTGCAGTATTCCTCGAG+AGG | 0.580542 | 1.4:-69383338 | None:intergenic |
| AAAGACGTCGGACCCTCTCG+AGG | 0.580550 | 1.4:+69383325 | MS.gene07287:CDS |
| CGCTAGCGGTGAATGTGTGG+CGG | 0.584497 | 1.4:+69383247 | MS.gene07287:CDS |
| GCGGTGAATGTGTGGCGGCG+TGG | 0.592428 | 1.4:+69383252 | MS.gene07287:CDS |
| GAACACCGGTCCAGACCTGA+TGG | 0.594707 | 1.4:-69383087 | None:intergenic |
| ATGTCCGGTGATGCTTGCAC+CGG | 0.596075 | 1.4:-69383167 | None:intergenic |
| TAGGATGGTAAGCATTCGAC+CGG | 0.597009 | 1.4:+69383148 | MS.gene07287:CDS |
| CCTTGCAGTATTCCTCGAGA+GGG | 0.604324 | 1.4:-69383337 | None:intergenic |
| ACCGGACATAACCGGGAAGG+TGG | 0.605824 | 1.4:+69383181 | MS.gene07287:CDS |
| GTACGACACTCATTAGCCTC+CGG | 0.606135 | 1.4:-69383365 | None:intergenic |
| TGTGCAGGAGACGCCGCTAG+CGG | 0.609103 | 1.4:+69383233 | MS.gene07287:CDS |
| TCGAATGCTTACCATCCTAC+CGG | 0.609712 | 1.4:-69383144 | None:intergenic |
| CCCTCTCGAGGAATACTGCA+AGG | 0.623920 | 1.4:+69383337 | MS.gene07287:CDS |
| CCGGTGCAGTCAGTTCCGGT+AGG | 0.639198 | 1.4:+69383129 | MS.gene07287:CDS |
| CTTTGGTTGAGCATGAACAC+CGG | 0.653169 | 1.4:-69383101 | None:intergenic |
| TGAGGTTGAGGAACTGAGTG+CGG | 0.653464 | 1.4:+69383277 | MS.gene07287:CDS |
| CCTACCGGAACTGACTGCAC+CGG | 0.656038 | 1.4:-69383129 | None:intergenic |
| ATCACCGGACATAACCGGGA+AGG | 0.669449 | 1.4:+69383178 | MS.gene07287:CDS |
| GGTTGAGGAACTGAGTGCGG+CGG | 0.687298 | 1.4:+69383280 | MS.gene07287:CDS |
| ATACTGCAAGGACAATCCGG+AGG | 0.732199 | 1.4:+69383349 | MS.gene07287:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGGATAAGAAAAAGACGT+CGG | + | chr1.4:69383313-69383332 | MS.gene07287:CDS | 35.0% | |
| GTCCCTTGAAAATAACTCTA+GGG | - | chr1.4:69383043-69383062 | None:intergenic | 35.0% | |
| ! | TCTTTTTCTTATCCCTTGCA+TGG | - | chr1.4:69383310-69383329 | None:intergenic | 35.0% |
| !! | AAGCCCTAGAGTTATTTTCA+AGG | + | chr1.4:69383037-69383056 | MS.gene07287:CDS | 35.0% |
| !! | AGCCCTAGAGTTATTTTCAA+GGG | + | chr1.4:69383038-69383057 | MS.gene07287:CDS | 35.0% |
| AGAGTCTCTTCAAAAGTCTC+AGG | + | chr1.4:69383064-69383083 | MS.gene07287:CDS | 40.0% | |
| GGTCCCTTGAAAATAACTCT+AGG | - | chr1.4:69383044-69383063 | None:intergenic | 40.0% | |
| TGTTCATGCTCAACCAAAGA+TGG | + | chr1.4:69383105-69383124 | MS.gene07287:CDS | 40.0% | |
| !! | TGAGACTTTTGAAGAGACTC+TGG | - | chr1.4:69383065-69383084 | None:intergenic | 40.0% |
| ATGCTCAACCAAAGATGGAC+CGG | + | chr1.4:69383110-69383129 | MS.gene07287:CDS | 45.0% | |
| CTTTGGTTGAGCATGAACAC+CGG | - | chr1.4:69383104-69383123 | None:intergenic | 45.0% | |
| GGAATACTGCAAGGACAATC+CGG | + | chr1.4:69383346-69383365 | MS.gene07287:CDS | 45.0% | |
| TAGGATGGTAAGCATTCGAC+CGG | + | chr1.4:69383148-69383167 | MS.gene07287:CDS | 45.0% | |
| TCGAATGCTTACCATCCTAC+CGG | - | chr1.4:69383147-69383166 | None:intergenic | 45.0% | |
| TTCAAAAGTCTCAGGCCATC+AGG | + | chr1.4:69383072-69383091 | MS.gene07287:CDS | 45.0% | |
| ! | GAGCATAAAGAGTGCAGAAG+AGG | + | chr1.4:69383208-69383227 | MS.gene07287:CDS | 45.0% |
| AAGCATCACCGGACATAACC+GGG | + | chr1.4:69383174-69383193 | MS.gene07287:CDS | 50.0% | |
| ATACTGCAAGGACAATCCGG+AGG | + | chr1.4:69383349-69383368 | MS.gene07287:CDS | 50.0% | |
| CAAGCATCACCGGACATAAC+CGG | + | chr1.4:69383173-69383192 | MS.gene07287:CDS | 50.0% | |
| CCTTGCAGTATTCCTCGAGA+GGG | - | chr1.4:69383340-69383359 | None:intergenic | 50.0% | |
| TATGCTCTCTTCCACCTTCC+CGG | - | chr1.4:69383195-69383214 | None:intergenic | 50.0% | |
| TCCTTGCAGTATTCCTCGAG+AGG | - | chr1.4:69383341-69383360 | None:intergenic | 50.0% | |
| TGAGGTTGAGGAACTGAGTG+CGG | + | chr1.4:69383277-69383296 | MS.gene07287:CDS | 50.0% | |
| ! | GTACGACACTCATTAGCCTC+CGG | - | chr1.4:69383368-69383387 | None:intergenic | 50.0% |
| AAGTCTCAGGCCATCAGGTC+TGG | + | chr1.4:69383077-69383096 | MS.gene07287:CDS | 55.0% | |
| ATCACCGGACATAACCGGGA+AGG | + | chr1.4:69383178-69383197 | MS.gene07287:CDS | 55.0% | |
| ATGTCCGGTGATGCTTGCAC+CGG | - | chr1.4:69383170-69383189 | None:intergenic | 55.0% | |
| CCCTCTCGAGGAATACTGCA+AGG | + | chr1.4:69383337-69383356 | MS.gene07287:CDS | 55.0% | |
| TCCACCTTCCCGGTTATGTC+CGG | - | chr1.4:69383185-69383204 | None:intergenic | 55.0% | |
| TGCAGTCAGTTCCGGTAGGA+TGG | + | chr1.4:69383133-69383152 | MS.gene07287:CDS | 55.0% | |
| ! | TGACTGCACCGGTCCATCTT+TGG | - | chr1.4:69383121-69383140 | None:intergenic | 55.0% |
| AAAGACGTCGGACCCTCTCG+AGG | + | chr1.4:69383325-69383344 | MS.gene07287:CDS | 60.0% | |
| ACCGGACATAACCGGGAAGG+TGG | + | chr1.4:69383181-69383200 | MS.gene07287:CDS | 60.0% | |
| CCTACCGGAACTGACTGCAC+CGG | - | chr1.4:69383132-69383151 | None:intergenic | 60.0% | |
| CGCCACACATTCACCGCTAG+CGG | - | chr1.4:69383249-69383268 | None:intergenic | 60.0% | |
| CGCTAGCGGTGAATGTGTGG+CGG | + | chr1.4:69383247-69383266 | MS.gene07287:CDS | 60.0% | |
| GAACACCGGTCCAGACCTGA+TGG | - | chr1.4:69383090-69383109 | None:intergenic | 60.0% | |
| GGTTGAGGAACTGAGTGCGG+CGG | + | chr1.4:69383280-69383299 | MS.gene07287:CDS | 60.0% | |
| TCAGGCCATCAGGTCTGGAC+CGG | + | chr1.4:69383082-69383101 | MS.gene07287:CDS | 60.0% | |
| TCGACCGGTGCAAGCATCAC+CGG | + | chr1.4:69383163-69383182 | MS.gene07287:CDS | 60.0% | |
| TGGACCGGTGCAGTCAGTTC+CGG | + | chr1.4:69383125-69383144 | MS.gene07287:CDS | 60.0% | |
| ! | AGTGCAGAAGAGGCGTGTGC+AGG | + | chr1.4:69383218-69383237 | MS.gene07287:CDS | 60.0% |
| ATGTGTGGCGGCGTGGGATG+AGG | + | chr1.4:69383259-69383278 | MS.gene07287:CDS | 65.0% | |
| CCGGTGCAGTCAGTTCCGGT+AGG | + | chr1.4:69383129-69383148 | MS.gene07287:CDS | 65.0% | |
| CGCCGCTAGCGGTGAATGTG+TGG | + | chr1.4:69383244-69383263 | MS.gene07287:CDS | 65.0% | |
| CGGTGAATGTGTGGCGGCGT+GGG | + | chr1.4:69383253-69383272 | MS.gene07287:CDS | 65.0% | |
| TGCGGCGGCAAGCCATGCAA+GGG | + | chr1.4:69383295-69383314 | MS.gene07287:CDS | 65.0% | |
| TGTGCAGGAGACGCCGCTAG+CGG | + | chr1.4:69383233-69383252 | MS.gene07287:CDS | 65.0% | |
| GCGGTGAATGTGTGGCGGCG+TGG | + | chr1.4:69383252-69383271 | MS.gene07287:CDS | 70.0% | |
| GGCGGCGTGGGATGAGGTTG+AGG | + | chr1.4:69383265-69383284 | MS.gene07287:CDS | 70.0% | |
| GTGCGGCGGCAAGCCATGCA+AGG | + | chr1.4:69383294-69383313 | MS.gene07287:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 69383005 | 69383400 | 69383005 | ID=MS.gene07287 |
| chr1.4 | mRNA | 69383005 | 69383400 | 69383005 | ID=MS.gene07287.t1;Parent=MS.gene07287 |
| chr1.4 | exon | 69383005 | 69383400 | 69383005 | ID=MS.gene07287.t1.exon1;Parent=MS.gene07287.t1 |
| chr1.4 | CDS | 69383005 | 69383400 | 69383005 | ID=cds.MS.gene07287.t1;Parent=MS.gene07287.t1 |
| Gene Sequence |
| Protein sequence |
