AlfalfaGEDB | MS.gene073052

M. sativa cultivar XinJiangDaYe / MS.gene073052

KEGG: K03439
GO: GO:0003674 GO:0003824 GO:0006139 GO:0006396 GO:0006399 GO:0006400 GO:0006725 GO:0006807 GO:0008033 GO:0008150 GO:0008152 GO:0008168 GO:0008173 GO:0008175 GO:0008176 GO:0008757 GO:0009451 GO:0009987 GO:0016070 GO:0016423 GO:0016740 GO:0016741 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360
PFAM: PF02390.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073052.t1	XP_003603585.1	98.8	255	3	0	1	255	1	255	2.90E-141	511.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073052.t1	Q8GXB7	79.8	243	46	2	8	249	8	248	2.5e-109	396.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073052.t1	G7J2W7	98.8	255	3	0	1	255	1	255	2.1e-141	511.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene073052	MS.gene038492	PPI
MS.gene073052	MS.gene048631	PPI
MS.gene018116	MS.gene073052	PPI
MS.gene073052	MS.gene40390	PPI
MS.gene073052	MS.gene50496	PPI
MS.gene073052	MS.gene71227	PPI
MS.gene073052	MS.gene61042	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073052.t1	MTR_3g109360	98.824	255	3	0	1	255	1	255	0.0	525

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073052.t1	AT5G24840	79.835	243	46	2	8	249	8	248	3.11e-146	409

Find 65 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATTAAGTTTGCCGATAT+TGG	0.221557	3.3:+89604360	MS.gene073052:CDS
TCCGCACCACAGAAACATTT+TGG	0.250656	3.3:-89604524	None:intergenic
ATTCCGATCATCAATGTTTC+TGG	0.252188	3.3:-89604426	None:intergenic
GCCGATATTGGCTGTGGTTT+TGG	0.274610	3.3:+89604372	MS.gene073052:CDS
CCTCGTTTCTAGCAACCTTT+TGG	0.326676	3.3:-89604854	None:intergenic
TAAAATGAGGATCGGGAAAC+AGG	0.368009	3.3:-89604608	None:intergenic
TTCTCTTTAAAATGAGGATC+GGG	0.398927	3.3:-89604615	None:intergenic
GCATACTCATCTAGTAAGAA+TGG	0.400409	3.3:-89604663	None:intergenic
AAACGAGGGACAAACTTTCC+AGG	0.404412	3.3:+89604869	MS.gene073052:CDS
TTGTGCTTTCTCAAAGTGAT+TGG	0.404689	3.3:-89604568	None:intergenic
GGACTGGATGAAATCTTGTT+TGG	0.409104	3.3:+89604743	MS.gene073052:CDS
TCCAAAACCACAGCCAATAT+CGG	0.417152	3.3:-89604373	None:intergenic
TTAAGCTCTGCCACTGAAGA+AGG	0.428496	3.3:+89604831	MS.gene073052:CDS
TGTGCTTTCTCAAAGTGATT+GGG	0.444654	3.3:-89604567	None:intergenic
TCAACATTCAGTAAGTCAAC+AGG	0.452366	3.3:+89604186	MS.gene073052:CDS
GTGATTGGGAATGTACTTCA+TGG	0.454613	3.3:-89604553	None:intergenic
GTTGAATTTCCACCAGTCTC+AGG	0.455044	3.3:-89604168	None:intergenic
TGGTTCTTCTCTTTAAAATG+AGG	0.455252	3.3:-89604621	None:intergenic
CAGTCAAAGGTTCAAACAAC+GGG	0.464877	3.3:-89604773	None:intergenic
TAAAGTGTCCGAGTATGTTA+AGG	0.471708	3.3:+89604461	MS.gene073052:CDS
TCAAGTTCTTTCTCAGTCAA+AGG	0.473439	3.3:-89604786	None:intergenic
ATAACAGATGTGGAAGAACT+TGG	0.476880	3.3:+89604720	MS.gene073052:CDS
GCTCGATAGAAGCGCTTTCG+AGG	0.477207	3.3:-89604213	None:intergenic
TCTTTAAGGGTAGCAAATCC+AGG	0.486768	3.3:+89604495	MS.gene073052:CDS
GAAAGAACTTGAAGCAGATC+CGG	0.487319	3.3:+89604797	MS.gene073052:CDS
TTCCGATCATCAATGTTTCT+GGG	0.488618	3.3:-89604425	None:intergenic
AGAGAAGAACCATCGCCGTC+GGG	0.492041	3.3:+89604632	MS.gene073052:CDS
GAGGGAATAGTCAACATGAC+TGG	0.493842	3.3:-89604289	None:intergenic
CTTCTCTTTAAAATGAGGAT+CGG	0.494124	3.3:-89604616	None:intergenic
CATACTCATCTAGTAAGAAT+GGG	0.503167	3.3:-89604662	None:intergenic
TGCCACTGAAGAAGGCCAAA+AGG	0.507812	3.3:+89604839	MS.gene073052:CDS
TCAACATGACTGGGTGATAA+CGG	0.513146	3.3:-89604279	None:intergenic
AAGTTTGCCGATATTGGCTG+TGG	0.514722	3.3:+89604366	MS.gene073052:CDS
TAACAGATGTGGAAGAACTT+GGG	0.526686	3.3:+89604721	MS.gene073052:CDS
GAAAGAACTGAGGGTAATGG+AGG	0.526775	3.3:-89604308	None:intergenic
CTATCAGCTTTCTCAGACAA+GGG	0.532860	3.3:-89604330	None:intergenic
AAGAGAAGAACCATCGCCGT+CGG	0.534336	3.3:+89604631	MS.gene073052:CDS
AGGGAAAGAACTGAGGGTAA+TGG	0.540719	3.3:-89604311	None:intergenic
CAATGCGTCTGTATACAGCC+TGG	0.550730	3.3:-89604887	None:intergenic
GGCTGATAACCCGACGGCGA+TGG	0.555787	3.3:-89604641	None:intergenic
TAAACAGACATGCCTGAGAC+TGG	0.565306	3.3:+89604156	None:intergenic
ACCAAAATGTTTCTGTGGTG+CGG	0.567556	3.3:+89604523	MS.gene073052:CDS
AATTCGTTCCTTAACATACT+CGG	0.570956	3.3:-89604469	None:intergenic
TTCCCAGAAACATTGATGAT+CGG	0.572410	3.3:+89604423	MS.gene073052:CDS
GAGCTTAAAAGCTTAACAGC+CGG	0.573038	3.3:-89604816	None:intergenic
ATGTGGAAGAACTTGGGGAC+TGG	0.574117	3.3:+89604727	MS.gene073052:CDS
TGACTGGGTGATAACGGCAC+AGG	0.576851	3.3:-89604273	None:intergenic
AGAAACATTGATGATCGGAA+TGG	0.582107	3.3:+89604428	MS.gene073052:CDS
AAAGAACTGAGGGTAATGGA+GGG	0.599329	3.3:-89604307	None:intergenic
TATATACACAATAACAGATG+TGG	0.600775	3.3:+89604710	MS.gene073052:CDS
AACAGATGTGGAAGAACTTG+GGG	0.605004	3.3:+89604722	MS.gene073052:CDS
GGAAATGAGAGTCACTGAGC+GGG	0.605858	3.3:-89604251	None:intergenic
GACTGGGTGATAACGGCACA+GGG	0.611699	3.3:-89604272	None:intergenic
ACTATCAGCTTTCTCAGACA+AGG	0.612778	3.3:-89604331	None:intergenic
TCAGTCAAAGGTTCAAACAA+CGG	0.617042	3.3:-89604774	None:intergenic
TCAATACCAAAATGTTTCTG+TGG	0.618646	3.3:+89604518	MS.gene073052:CDS
CCAAAAGGTTGCTAGAAACG+AGG	0.621682	3.3:+89604854	MS.gene073052:CDS
AGGGAATAGTCAACATGACT+GGG	0.631622	3.3:-89604288	None:intergenic
ACAGACATGCCTGAGACTGG+TGG	0.638512	3.3:+89604159	None:intergenic
GGGAAATGAGAGTCACTGAG+CGG	0.640601	3.3:-89604252	None:intergenic
CAAAAGGTTGCTAGAAACGA+GGG	0.641444	3.3:+89604855	MS.gene073052:CDS
CAGACAAGGGAAAGAACTGA+GGG	0.645415	3.3:-89604317	None:intergenic
CAATGTTTCTGGGAACAGAG+TGG	0.655902	3.3:-89604415	None:intergenic
TCAGACAAGGGAAAGAACTG+AGG	0.682631	3.3:-89604318	None:intergenic
AGAATGGGCTGATAACCCGA+CGG	0.724299	3.3:-89604647	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TATATACACAATAACAGATG+TGG	+	chr3.3:89604710-89604729	MS.gene073052:CDS	25.0%
!!	AGGAACGAATTTTATCTTTA+AGG	+	chr3.3:89604481-89604500	MS.gene073052:CDS	25.0%
!!	GGAACGAATTTTATCTTTAA+GGG	+	chr3.3:89604482-89604501	MS.gene073052:CDS	25.0%
	AAGATTAAGTTTGCCGATAT+TGG	+	chr3.3:89604360-89604379	MS.gene073052:CDS	30.0%
	AATTCGTTCCTTAACATACT+CGG	-	chr3.3:89604472-89604491	None:intergenic	30.0%
	CATACTCATCTAGTAAGAAT+GGG	-	chr3.3:89604665-89604684	None:intergenic	30.0%
!	TATGCTTTTGTTCTTGAGAT+TGG	+	chr3.3:89604681-89604700	MS.gene073052:CDS	30.0%
!	TCAATACCAAAATGTTTCTG+TGG	+	chr3.3:89604518-89604537	MS.gene073052:CDS	30.0%
!!	CTTCTCTTTAAAATGAGGAT+CGG	-	chr3.3:89604619-89604638	None:intergenic	30.0%
!!	TGGTTCTTCTCTTTAAAATG+AGG	-	chr3.3:89604624-89604643	None:intergenic	30.0%
!!	TTCTCTTTAAAATGAGGATC+GGG	-	chr3.3:89604618-89604637	None:intergenic	30.0%
	ATAACAGATGTGGAAGAACT+TGG	+	chr3.3:89604720-89604739	MS.gene073052:CDS	35.0%
	ATTCCGATCATCAATGTTTC+TGG	-	chr3.3:89604429-89604448	None:intergenic	35.0%
	GCATACTCATCTAGTAAGAA+TGG	-	chr3.3:89604666-89604685	None:intergenic	35.0%
	TAACAGATGTGGAAGAACTT+GGG	+	chr3.3:89604721-89604740	MS.gene073052:CDS	35.0%
	TCAACATTCAGTAAGTCAAC+AGG	+	chr3.3:89604186-89604205	MS.gene073052:CDS	35.0%
	TCAAGTTCTTTCTCAGTCAA+AGG	-	chr3.3:89604789-89604808	None:intergenic	35.0%
	TCAGTCAAAGGTTCAAACAA+CGG	-	chr3.3:89604777-89604796	None:intergenic	35.0%
	TGTGCTTTCTCAAAGTGATT+GGG	-	chr3.3:89604570-89604589	None:intergenic	35.0%
	TTCCGATCATCAATGTTTCT+GGG	-	chr3.3:89604428-89604447	None:intergenic	35.0%
	TTGTGCTTTCTCAAAGTGAT+TGG	-	chr3.3:89604571-89604590	None:intergenic	35.0%
!	AGAAACATTGATGATCGGAA+TGG	+	chr3.3:89604428-89604447	MS.gene073052:CDS	35.0%
!	TAAAGTGTCCGAGTATGTTA+AGG	+	chr3.3:89604461-89604480	MS.gene073052:CDS	35.0%
!	TTCCCAGAAACATTGATGAT+CGG	+	chr3.3:89604423-89604442	MS.gene073052:CDS	35.0%
!!!	GAAACATTTTGGTATTGACC+TGG	-	chr3.3:89604516-89604535	None:intergenic	35.0%
	AACAGATGTGGAAGAACTTG+GGG	+	chr3.3:89604722-89604741	MS.gene073052:CDS	40.0%
	ACTATCAGCTTTCTCAGACA+AGG	-	chr3.3:89604334-89604353	None:intergenic	40.0%
	AGGGAATAGTCAACATGACT+GGG	-	chr3.3:89604291-89604310	None:intergenic	40.0%
	CAAAAGGTTGCTAGAAACGA+GGG	+	chr3.3:89604855-89604874	MS.gene073052:CDS	40.0%
	CAGTCAAAGGTTCAAACAAC+GGG	-	chr3.3:89604776-89604795	None:intergenic	40.0%
	CTATCAGCTTTCTCAGACAA+GGG	-	chr3.3:89604333-89604352	None:intergenic	40.0%
	GAAAGAACTTGAAGCAGATC+CGG	+	chr3.3:89604797-89604816	MS.gene073052:CDS	40.0%
	GAGCTTAAAAGCTTAACAGC+CGG	-	chr3.3:89604819-89604838	None:intergenic	40.0%
	GGACTGGATGAAATCTTGTT+TGG	+	chr3.3:89604743-89604762	MS.gene073052:CDS	40.0%
	GTGATTGGGAATGTACTTCA+TGG	-	chr3.3:89604556-89604575	None:intergenic	40.0%
	TAAAATGAGGATCGGGAAAC+AGG	-	chr3.3:89604611-89604630	None:intergenic	40.0%
	TCAACATGACTGGGTGATAA+CGG	-	chr3.3:89604282-89604301	None:intergenic	40.0%
	TCCAAAACCACAGCCAATAT+CGG	-	chr3.3:89604376-89604395	None:intergenic	40.0%
!	ACCAAAATGTTTCTGTGGTG+CGG	+	chr3.3:89604523-89604542	MS.gene073052:CDS	40.0%
!	GCTTTTGTTCTTGAGATTGG+TGG	+	chr3.3:89604684-89604703	MS.gene073052:CDS	40.0%
!	TCTTTAAGGGTAGCAAATCC+AGG	+	chr3.3:89604495-89604514	MS.gene073052:CDS	40.0%
!!	AAAGAACTGAGGGTAATGGA+GGG	-	chr3.3:89604310-89604329	None:intergenic	40.0%
!!!	ATATTGGCTGTGGTTTTGGA+GGG	+	chr3.3:89604376-89604395	MS.gene073052:CDS	40.0%
	AAACGAGGGACAAACTTTCC+AGG	+	chr3.3:89604869-89604888	MS.gene073052:CDS	45.0%
	AGGGAAAGAACTGAGGGTAA+TGG	-	chr3.3:89604314-89604333	None:intergenic	45.0%
	CAGACAAGGGAAAGAACTGA+GGG	-	chr3.3:89604320-89604339	None:intergenic	45.0%
	CCAAAAGGTTGCTAGAAACG+AGG	+	chr3.3:89604854-89604873	MS.gene073052:CDS	45.0%
	GAGGGAATAGTCAACATGAC+TGG	-	chr3.3:89604292-89604311	None:intergenic	45.0%
	GTTGAATTTCCACCAGTCTC+AGG	-	chr3.3:89604171-89604190	None:intergenic	45.0%
	TCAGACAAGGGAAAGAACTG+AGG	-	chr3.3:89604321-89604340	None:intergenic	45.0%
	TTAAGCTCTGCCACTGAAGA+AGG	+	chr3.3:89604831-89604850	MS.gene073052:CDS	45.0%
!	AAGTTTGCCGATATTGGCTG+TGG	+	chr3.3:89604366-89604385	MS.gene073052:CDS	45.0%
!	GAAAGAACTGAGGGTAATGG+AGG	-	chr3.3:89604311-89604330	None:intergenic	45.0%
!	TCCGCACCACAGAAACATTT+TGG	-	chr3.3:89604527-89604546	None:intergenic	45.0%
!!	AACCTTTTGGCCTTCTTCAG+TGG	-	chr3.3:89604844-89604863	None:intergenic	45.0%
!!	CAATGTTTCTGGGAACAGAG+TGG	-	chr3.3:89604418-89604437	None:intergenic	45.0%
!!!	CCTCGTTTCTAGCAACCTTT+TGG	-	chr3.3:89604857-89604876	None:intergenic	45.0%
!!!	GATATTGGCTGTGGTTTTGG+AGG	+	chr3.3:89604375-89604394	MS.gene073052:CDS	45.0%
	AAGAGAAGAACCATCGCCGT+CGG	+	chr3.3:89604631-89604650	MS.gene073052:CDS	50.0%
	ATGTGGAAGAACTTGGGGAC+TGG	+	chr3.3:89604727-89604746	MS.gene073052:CDS	50.0%
	CAATGCGTCTGTATACAGCC+TGG	-	chr3.3:89604890-89604909	None:intergenic	50.0%
	GGAAATGAGAGTCACTGAGC+GGG	-	chr3.3:89604254-89604273	None:intergenic	50.0%
	GGGAAATGAGAGTCACTGAG+CGG	-	chr3.3:89604255-89604274	None:intergenic	50.0%
	TGCCACTGAAGAAGGCCAAA+AGG	+	chr3.3:89604839-89604858	MS.gene073052:CDS	50.0%
!!	AGAATGGGCTGATAACCCGA+CGG	-	chr3.3:89604650-89604669	None:intergenic	50.0%
!!	GCCGATATTGGCTGTGGTTT+TGG	+	chr3.3:89604372-89604391	MS.gene073052:CDS	50.0%
	AGAGAAGAACCATCGCCGTC+GGG	+	chr3.3:89604632-89604651	MS.gene073052:CDS	55.0%
!!	GACTGGGTGATAACGGCACA+GGG	-	chr3.3:89604275-89604294	None:intergenic	55.0%
!!	GCTCGATAGAAGCGCTTTCG+AGG	-	chr3.3:89604216-89604235	None:intergenic	55.0%
!!	TGACTGGGTGATAACGGCAC+AGG	-	chr3.3:89604276-89604295	None:intergenic	55.0%
	GGCTGATAACCCGACGGCGA+TGG	-	chr3.3:89604644-89604663	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	89604165	89604932	89604165	ID=MS.gene073052
chr3.3	mRNA	89604165	89604932	89604165	ID=MS.gene073052.t1;Parent=MS.gene073052
chr3.3	exon	89604165	89604932	89604165	ID=MS.gene073052.t1.exon1;Parent=MS.gene073052.t1
chr3.3	CDS	89604165	89604932	89604165	ID=cds.MS.gene073052.t1;Parent=MS.gene073052.t1

Gene Sequence

>MS.gene073052

ATGCCTGAGACTGGTGGAAATTCAACATTCAGTAAGTCAACAGGATTGCCTCGAAAGCGCTTCTATCGAGCACGAGCACACAGCAACCCGCTCAGTGACTCTCATTTCCCTGTGCCGTTATCACCCAGTCATGTTGACTATTCCCTCCATTACCCTCAGTTCTTTCCCTTGTCTGAGAAAGCTGATAGTTGCAAAAAGATTAAGTTTGCCGATATTGGCTGTGGTTTTGGAGGGTTGCTCATCAGTCTTTCCACTCTGTTCCCAGAAACATTGATGATCGGAATGGAACTTAGAGATAAAGTGTCCGAGTATGTTAAGGAACGAATTTTATCTTTAAGGGTAGCAAATCCAGGTCAATACCAAAATGTTTCTGTGGTGCGGACTAACTCCATGAAGTACATTCCCAATCACTTTGAGAAAGCACAACTCTCAAAGATGTTTTTCCTGTTTCCCGATCCTCATTTTAAAGAGAAGAACCATCGCCGTCGGGTTATCAGCCCATTCTTACTAGATGAGTATGCTTTTGTTCTTGAGATTGGTGGAATTATATACACAATAACAGATGTGGAAGAACTTGGGGACTGGATGAAATCTTGTTTGGAGAATCACCCGTTGTTTGAACCTTTGACTGAGAAAGAACTTGAAGCAGATCCGGCTGTTAAGCTTTTAAGCTCTGCCACTGAAGAAGGCCAAAAGGTTGCTAGAAACGAGGGACAAACTTTCCAGGCTGTATACAGACGCATTGCAGCATCTGATCAAATATCCTAG

Protein sequence

>MS.gene073052.t1

MPETGGNSTFSKSTGLPRKRFYRARAHSNPLSDSHFPVPLSPSHVDYSLHYPQFFPLSEKADSCKKIKFADIGCGFGGLLISLSTLFPETLMIGMELRDKVSEYVKERILSLRVANPGQYQNVSVVRTNSMKYIPNHFEKAQLSKMFFLFPDPHFKEKNHRRRVISPFLLDEYAFVLEIGGIIYTITDVEELGDWMKSCLENHPLFEPLTEKELEADPAVKLLSSATEEGQKVARNEGQTFQAVYRRIAASDQIS