AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073146

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073146.t1 MTR_2g084280 95.161 310 11 1 3 308 4 313 0.0 606
MS.gene073146.t1 MTR_4g057270 63.804 326 91 10 2 308 16 333 2.73e-129 372
MS.gene073146.t1 MTR_8g064700 33.962 159 84 3 120 264 236 387 8.48e-20 89.4
MS.gene073146.t1 MTR_3g104100 31.771 192 110 4 127 304 194 378 5.98e-18 83.6
MS.gene073146.t1 MTR_3g498695 30.387 181 110 4 88 253 257 436 3.12e-17 82.0
MS.gene073146.t1 MTR_2g038040 25.112 223 127 5 91 295 34 234 3.17e-17 79.7
MS.gene073146.t1 MTR_3g498695 30.387 181 110 4 88 253 257 436 3.18e-17 82.0
MS.gene073146.t1 MTR_1g112680 30.233 172 86 4 116 253 302 473 4.31e-17 82.0
MS.gene073146.t1 MTR_3g030700 29.744 195 116 4 117 297 185 372 7.58e-17 80.5
MS.gene073146.t1 MTR_8g067280 30.864 162 93 5 132 277 466 624 4.52e-15 75.9
MS.gene073146.t1 MTR_2g081040 30.270 185 113 6 127 297 176 358 1.55e-14 73.6
MS.gene073146.t1 MTR_5g030430 30.488 164 91 5 132 277 497 655 2.42e-14 73.9
MS.gene073146.t1 MTR_8g027495 29.605 152 97 4 132 281 462 605 2.96e-14 73.6
MS.gene073146.t1 MTR_4g091910 30.357 168 110 4 116 282 37 198 6.56e-14 69.7
MS.gene073146.t1 MTR_4g097950 31.111 135 79 3 130 252 72 204 1.17e-13 69.7
MS.gene073146.t1 MTR_7g117670 29.333 150 100 2 132 281 406 549 1.27e-13 71.6
MS.gene073146.t1 MTR_7g083900 29.139 151 87 4 127 260 285 432 2.89e-13 70.5
MS.gene073146.t1 MTR_7g083900 29.139 151 87 4 127 260 285 432 3.00e-13 70.1
MS.gene073146.t1 MTR_8g024770 33.708 89 54 1 132 215 283 371 1.14e-11 65.5
MS.gene073146.t1 MTR_1g019240 34.579 107 63 3 78 180 44 147 1.32e-11 64.3
MS.gene073146.t1 MTR_4g092700 28.777 139 93 4 130 264 160 296 1.49e-11 64.7
MS.gene073146.t1 MTR_5g014640 29.323 133 93 1 130 262 156 287 4.98e-11 63.2
MS.gene073146.t1 MTR_8g024790 26.897 145 97 4 132 270 295 436 7.31e-11 63.2
MS.gene073146.t1 MTR_7g096350 26.667 150 88 4 132 281 359 486 9.50e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073146.t1 AT2G28160 52.905 327 121 9 1 308 5 317 2.15e-104 308
MS.gene073146.t1 AT2G28160 52.336 321 120 9 1 302 5 311 2.37e-100 297
MS.gene073146.t1 AT4G29930 32.836 201 119 4 99 299 36 220 2.58e-25 101
MS.gene073146.t1 AT4G29930 32.836 201 116 5 99 299 36 217 2.92e-24 98.6
MS.gene073146.t1 AT5G57150 32.828 198 112 6 110 291 34 226 2.19e-23 96.7
MS.gene073146.t1 AT4G29930 33.333 180 103 5 99 278 36 198 2.93e-23 96.3
MS.gene073146.t1 AT5G57150 31.111 225 128 7 70 278 3 216 3.14e-23 95.9
MS.gene073146.t1 AT5G57150 32.227 211 116 7 70 264 3 202 4.65e-23 95.1
MS.gene073146.t1 AT5G57150 32.381 210 116 7 70 264 3 201 5.50e-23 95.1
MS.gene073146.t1 AT5G57150 32.057 209 119 6 70 264 3 202 5.91e-23 95.9
MS.gene073146.t1 AT4G29930 30.396 227 116 7 99 299 36 246 2.74e-21 91.3
MS.gene073146.t1 AT2G16910 35.404 161 78 4 126 261 312 471 1.19e-20 92.4
MS.gene073146.t1 AT4G29930 30.244 205 103 6 99 277 36 226 4.01e-20 88.2
MS.gene073146.t1 AT4G29930 30.244 205 103 6 99 277 36 226 4.01e-20 88.2
MS.gene073146.t1 AT2G46510 29.508 183 104 6 109 282 368 534 4.41e-17 82.0
MS.gene073146.t1 AT1G01260 32.432 185 94 5 102 275 396 560 6.38e-17 81.6
MS.gene073146.t1 AT1G01260 32.432 185 94 5 102 275 396 560 6.38e-17 81.6
MS.gene073146.t1 AT1G01260 32.432 185 94 5 102 275 396 560 6.38e-17 81.6
MS.gene073146.t1 AT4G29930 38.776 98 49 1 99 196 36 122 8.89e-16 74.7
MS.gene073146.t1 AT4G17880 28.667 150 101 4 132 277 420 567 1.81e-14 74.3
MS.gene073146.t1 AT1G12860 46.970 66 35 0 127 192 266 331 4.07e-13 69.7
MS.gene073146.t1 AT1G12860 46.970 66 35 0 127 192 266 331 4.80e-13 69.7
MS.gene073146.t1 AT3G26744 29.932 147 89 3 127 260 306 451 8.41e-13 68.9
MS.gene073146.t1 AT3G26744 29.932 147 89 3 127 260 306 451 8.65e-13 68.9
MS.gene073146.t1 AT3G26744 29.932 147 89 3 127 260 306 451 8.65e-13 68.9
MS.gene073146.t1 AT3G26744 29.932 147 89 3 127 260 306 451 8.65e-13 68.9
MS.gene073146.t1 AT5G46760 29.091 165 85 6 132 277 419 570 4.95e-12 67.0
MS.gene073146.t1 AT1G32640 29.333 150 101 4 132 277 456 604 5.92e-12 66.6
MS.gene073146.t1 AT4G37850 26.531 245 145 11 73 299 99 326 5.22e-11 63.2
MS.gene073146.t1 AT4G37850 26.531 245 145 11 73 299 99 326 5.22e-11 63.2
MS.gene073146.t1 AT1G10610 45.161 62 34 0 126 187 165 226 6.77e-11 62.8
MS.gene073146.t1 AT2G22750 26.020 196 131 5 83 269 108 298 7.00e-11 62.8
MS.gene073146.t1 AT2G22750 26.020 196 131 5 83 269 112 302 7.58e-11 62.4
MS.gene073146.t1 AT2G22750 26.020 196 131 5 83 269 112 302 8.28e-11 62.4
MS.gene073146.t1 AT2G22750 26.020 196 131 5 83 269 84 274 8.59e-11 62.4
MS.gene073146.t1 AT2G22750 26.020 196 131 5 83 269 84 274 8.81e-11 62.0
MS.gene073146.t1 AT1G10610 45.161 62 34 0 126 187 232 293 9.21e-11 62.8

Find 57 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGTTGAATGCTGAGGTTTC+AGG 0.231415 2.2:-13417316 MS.gene073146:CDS
TATTATGATGGAGAGGTTAA+AGG 0.240976 2.2:-13417865 MS.gene073146:CDS
TGGATGGCTTTGAACATTAA+TGG 0.295160 2.2:+13417240 None:intergenic
GCTTTATGCATTGCGTTCTT+TGG 0.304101 2.2:-13417710 MS.gene073146:CDS
ATGGATAAGGCCTCTATAAT+TGG 0.313859 2.2:-13417382 MS.gene073146:CDS
TCGATAAATTGATCAAAGTT+TGG 0.327610 2.2:+13418084 None:intergenic
GTCATGCAGCTCAAAGTTAT+TGG 0.328211 2.2:+13418119 None:intergenic
CTAATTACCTTTGTAATGTT+GGG 0.360034 2.2:+13417685 None:intergenic
ACTAATTACCTTTGTAATGT+TGG 0.365158 2.2:+13417684 None:intergenic
ACTTCCTAGCTTGTGCTTGA+AGG 0.382012 2.2:+13417335 None:intergenic
ACCTTCAAAATTTGAAGCTA+TGG 0.386796 2.2:-13416433 MS.gene073146:CDS
GAGCCAATTGTTGTAGGGCT+TGG 0.390918 2.2:+13417895 None:intergenic
TTGGAGGAATTGAAAGAAGT+TGG 0.405788 2.2:+13417977 None:intergenic
GGCTCCTTCTCTTATTATGA+TGG 0.412262 2.2:-13417877 MS.gene073146:CDS
CAATTTATCGATTTAATTCG+AGG 0.430612 2.2:-13418072 MS.gene073146:CDS
AATCCAAGCCCTACAACAAT+TGG 0.433423 2.2:-13417898 MS.gene073146:CDS
CTGAGAGGAGAAGGAGAGGA+AGG 0.455802 2.2:-13417744 MS.gene073146:CDS
GAGAAGGAGCCAATTGTTGT+AGG 0.465494 2.2:+13417889 None:intergenic
GAGAAGGAGAGGAAGGATGA+AGG 0.465670 2.2:-13417737 MS.gene073146:CDS
GAAGATGCAATATCTAACTT+TGG 0.473277 2.2:-13418042 MS.gene073146:CDS
CCTTCAAAATTTGAAGCTAT+GGG 0.474141 2.2:-13416432 MS.gene073146:CDS
CTCTCATTTCTGAGAGGAGA+AGG 0.475201 2.2:-13417753 MS.gene073146:CDS
CCTCTCAGAAATGAGAGTCT+TGG 0.475407 2.2:+13417759 None:intergenic
TGGTGATCAAATGATGGGTT+TGG 0.476539 2.2:+13417958 None:intergenic
TCTGTGTCTGAAAATTATCA+AGG 0.477249 2.2:-13417277 MS.gene073146:CDS
AATTTGCAAGAAAATTATCC+AGG 0.484959 2.2:-13417218 MS.gene073146:intron
GATGGAGAGGTTAAAGGAGA+AGG 0.491246 2.2:-13417859 MS.gene073146:CDS
CAATGAAGCAATCATTGATA+AGG 0.492027 2.2:+13418013 None:intergenic
ATGGCATGAATTCAAAGCCT+TGG 0.500719 2.2:+13416390 None:intergenic
CATGCAGTGGCTCAAAATGA+TGG 0.501968 2.2:+13416371 None:intergenic
CGATCGATGATGCCAACCCT+AGG 0.507369 2.2:-13417798 MS.gene073146:CDS
AGAAGGAGCCAATTGTTGTA+GGG 0.509001 2.2:+13417890 None:intergenic
TATCCACATACAGATGGATA+AGG 0.511962 2.2:-13417395 MS.gene073146:intron
TACTGCATCTCCAATTATAG+AGG 0.518020 2.2:+13417372 None:intergenic
TTGTTGTGGTGATCAAATGA+TGG 0.518026 2.2:+13417952 None:intergenic
TATTATTAATAATATTGTTG+TGG 0.538958 2.2:+13417938 None:intergenic
AAAGGAGAAGGAGAAAATGA+TGG 0.542463 2.2:-13417847 MS.gene073146:CDS
GAAGGAGAAAATGATGGAGG+TGG 0.543991 2.2:-13417841 MS.gene073146:CDS
ATGTTTCAAGTAGAGGAAAG+AGG 0.546448 2.2:-13416735 MS.gene073146:CDS
CTTCTCTTATTATGATGGAG+AGG 0.551672 2.2:-13417872 MS.gene073146:CDS
ACCCTAGGGCTAAAACTGAT+AGG 0.553134 2.2:-13417783 MS.gene073146:CDS
GATGGACATGTTTCAAGTAG+AGG 0.555283 2.2:-13416742 MS.gene073146:CDS
AATTTGAAGCTATGGGTGGC+TGG 0.562736 2.2:-13416425 MS.gene073146:CDS
GGTGTTCTCTAGTGAGTCCA+TGG 0.563816 2.2:+13418140 None:intergenic
ATGACCTTCAAGCACAAGCT+AGG 0.564350 2.2:-13417339 MS.gene073146:CDS
ATAATGTGCAATAAAGGAGA+AGG 0.570295 2.2:-13416699 MS.gene073146:CDS
AGTAGCTGCTTCATTGTACA+AGG 0.575970 2.2:-13416676 MS.gene073146:CDS
TGATCAAATGATGGGTTTGG+AGG 0.577130 2.2:+13417961 None:intergenic
TGTTGTGGTGATCAAATGAT+GGG 0.582900 2.2:+13417953 None:intergenic
CTCTCCATCATAATAAGAGA+AGG 0.586757 2.2:+13417873 None:intergenic
ATTTCTGAGAGGAGAAGGAG+AGG 0.591195 2.2:-13417748 MS.gene073146:CDS
TTTGGTTCCCAACATTACAA+AGG 0.593392 2.2:-13417692 MS.gene073146:intron
GATCGATGATGCCAACCCTA+GGG 0.596237 2.2:-13417797 MS.gene073146:CDS
GGAGAAGGAGAAAATGATGG+AGG 0.607003 2.2:-13417844 MS.gene073146:CDS
AGCTAGGAAGTTGAATGCTG+AGG 0.609235 2.2:-13417323 MS.gene073146:CDS
TCAAAATTTGAAGCTATGGG+TGG 0.609799 2.2:-13416429 MS.gene073146:CDS
CCAAGACTCTCATTTCTGAG+AGG 0.669057 2.2:-13417759 MS.gene073146:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTATTAATAATATTGTTG+TGG + chr2.2:13416585-13416604 None:intergenic 10.0%
!!! AAAGTTTTTTCTTTATTATA+TGG + chr2.2:13417409-13417428 None:intergenic 10.0%
!!! CTAATTATAATTAGTTTTTA+AGG + chr2.2:13416927-13416946 None:intergenic 10.0%
!!! AATTCATTGATTAATGATTA+GGG - chr2.2:13417984-13418003 MS.gene073146:CDS 15.0%
!!! TTGTAATTTTCTATCTTTTT+TGG + chr2.2:13417636-13417655 None:intergenic 15.0%
!! AATGAAAAATTAAACAAACC+TGG + chr2.2:13417323-13417342 None:intergenic 20.0%
!! AGATAGAAAATTACAAACAA+AGG - chr2.2:13417640-13417659 MS.gene073146:intron 20.0%
!! ATTGATGTGTTGAAAAATAT+AGG - chr2.2:13418040-13418059 MS.gene073146:CDS 20.0%
!! CTAATCATTAATCAATGAAT+TGG + chr2.2:13417985-13418004 None:intergenic 20.0%
!! TGTTGAAAAATATAGGTAAA+CGG - chr2.2:13418047-13418066 MS.gene073146:CDS 20.0%
!! TTCGAAATTAATCGAAATAA+AGG + chr2.2:13416864-13416883 None:intergenic 20.0%
!!! AAAAATGTTGGTTTAAGATA+AGG + chr2.2:13417472-13417491 None:intergenic 20.0%
!!! AAGTTGCAAAGATATAATTT+TGG - chr2.2:13416969-13416988 MS.gene073146:intron 20.0%
!!! ATATGCTTGATATTAATTTG+AGG - chr2.2:13417064-13417083 MS.gene073146:intron 20.0%
!!! CAATTCATTGATTAATGATT+AGG - chr2.2:13417983-13418002 MS.gene073146:CDS 20.0%
!!! TAATTTTCTATCTTTTTTGG+TGG + chr2.2:13417633-13417652 None:intergenic 20.0%
!!! TTTTTTTTTTTTTGCACTAG+AGG - chr2.2:13417010-13417029 MS.gene073146:intron 20.0%
! AAAAACCAACTATAAAGTGT+TGG + chr2.2:13417713-13417732 None:intergenic 25.0%
! AACTTTACATTTGCAACTTA+AGG + chr2.2:13416954-13416973 None:intergenic 25.0%
! AATTTGCAAGAAAATTATCC+AGG - chr2.2:13417302-13417321 MS.gene073146:CDS 25.0%
! ACTAATTACCTTTGTAATGT+TGG + chr2.2:13416839-13416858 None:intergenic 25.0%
! ACTTTACATTTGCAACTTAA+GGG + chr2.2:13416953-13416972 None:intergenic 25.0%
! AGACTCATGAAATTATAACT+TGG + chr2.2:13417380-13417399 None:intergenic 25.0%
! CTAATTACCTTTGTAATGTT+GGG + chr2.2:13416838-13416857 None:intergenic 25.0%
! GCAAAAATAATGTGCAATAA+AGG - chr2.2:13417815-13417834 MS.gene073146:CDS 25.0%
! TCGATAAATTGATCAAAGTT+TGG + chr2.2:13416439-13416458 None:intergenic 25.0%
! TTGTCGTTAAGAAAAAATGT+TGG + chr2.2:13417484-13417503 None:intergenic 25.0%
! TTTATATATCCACATACAGA+TGG - chr2.2:13417119-13417138 MS.gene073146:intron 25.0%
!! TAATTTTCTTGCAAATTGGA+TGG + chr2.2:13417299-13417318 None:intergenic 25.0%
!! TGGATAATTTTCTTGCAAAT+TGG + chr2.2:13417303-13417322 None:intergenic 25.0%
!!! AAATTTTGAAGGTTCATTTC+TGG + chr2.2:13418080-13418099 None:intergenic 25.0%
!!! CAATTTATCGATTTAATTCG+AGG - chr2.2:13416448-13416467 MS.gene073146:CDS 25.0%
!!! GTTATTGATGTTTTTGCTTA+AGG - chr2.2:13418006-13418025 MS.gene073146:CDS 25.0%
AAACAAAGGACATCGTATAT+AGG - chr2.2:13417654-13417673 MS.gene073146:intron 30.0%
ATAATGTGCAATAAAGGAGA+AGG - chr2.2:13417821-13417840 MS.gene073146:CDS 30.0%
CAATGAAGCAATCATTGATA+AGG + chr2.2:13416510-13416529 None:intergenic 30.0%
GAAGATGCAATATCTAACTT+TGG - chr2.2:13416478-13416497 MS.gene073146:intron 30.0%
TCAATGAATTGGATATTGAG+AGG + chr2.2:13417974-13417993 None:intergenic 30.0%
TCTGTGTCTGAAAATTATCA+AGG - chr2.2:13417243-13417262 MS.gene073146:CDS 30.0%
TTAGACCAACACTTTATAGT+TGG - chr2.2:13417705-13417724 MS.gene073146:CDS 30.0%
! ACCTTCAAAATTTGAAGCTA+TGG - chr2.2:13418087-13418106 MS.gene073146:CDS 30.0%
! CCTTCAAAATTTGAAGCTAT+GGG - chr2.2:13418088-13418107 MS.gene073146:CDS 30.0%
! TATTATGATGGAGAGGTTAA+AGG - chr2.2:13416655-13416674 MS.gene073146:CDS 30.0%
!!! TTTTTTTGCACTAGAGGTTA+GGG - chr2.2:13417016-13417035 MS.gene073146:intron 30.0%
!!! TTTTTTTTGCACTAGAGGTT+AGG - chr2.2:13417015-13417034 MS.gene073146:intron 30.0%
AAAGGAGAAGGAGAAAATGA+TGG - chr2.2:13416673-13416692 MS.gene073146:CDS 35.0%
ATGGATAAGGCCTCTATAAT+TGG - chr2.2:13417138-13417157 MS.gene073146:intron 35.0%
CATAATATATGCAAGGTCTG+GGG - chr2.2:13417592-13417611 MS.gene073146:intron 35.0%
CTCTCCATCATAATAAGAGA+AGG + chr2.2:13416650-13416669 None:intergenic 35.0%
GACAGTGCATAATATATGCA+AGG - chr2.2:13417585-13417604 MS.gene073146:intron 35.0%
GCATAATATATGCAAGGTCT+GGG - chr2.2:13417591-13417610 MS.gene073146:intron 35.0%
TACTGCATCTCCAATTATAG+AGG + chr2.2:13417151-13417170 None:intergenic 35.0%
TATCCACATACAGATGGATA+AGG - chr2.2:13417125-13417144 MS.gene073146:intron 35.0%
TGCATAATATATGCAAGGTC+TGG - chr2.2:13417590-13417609 MS.gene073146:intron 35.0%
TGTGCATTATGCTATGTAGA+TGG - chr2.2:13417760-13417779 MS.gene073146:CDS 35.0%
TGTTGTGGTGATCAAATGAT+GGG + chr2.2:13416570-13416589 None:intergenic 35.0%
TTGGAGGAATTGAAAGAAGT+TGG + chr2.2:13416546-13416565 None:intergenic 35.0%
TTGTTGTGGTGATCAAATGA+TGG + chr2.2:13416571-13416590 None:intergenic 35.0%
! ATGTTTCAAGTAGAGGAAAG+AGG - chr2.2:13417785-13417804 MS.gene073146:CDS 35.0%
! CCCATAGCTTCAAATTTTGA+AGG + chr2.2:13418091-13418110 None:intergenic 35.0%
! CTTCTCTTATTATGATGGAG+AGG - chr2.2:13416648-13416667 MS.gene073146:CDS 35.0%
! TCAAAATTTGAAGCTATGGG+TGG - chr2.2:13418091-13418110 MS.gene073146:CDS 35.0%
! TTTGGTTCCCAACATTACAA+AGG - chr2.2:13416828-13416847 MS.gene073146:intron 35.0%
!! TGGATGGCTTTGAACATTAA+TGG + chr2.2:13417283-13417302 None:intergenic 35.0%
AAGTTGAATGCTGAGGTTTC+AGG - chr2.2:13417204-13417223 MS.gene073146:intron 40.0%
AATCCAAGCCCTACAACAAT+TGG - chr2.2:13416622-13416641 MS.gene073146:CDS 40.0%
AGAAGGAGCCAATTGTTGTA+GGG + chr2.2:13416633-13416652 None:intergenic 40.0%
AGTAGCTGCTTCATTGTACA+AGG - chr2.2:13417844-13417863 MS.gene073146:CDS 40.0%
ATGGCATGAATTCAAAGCCT+TGG + chr2.2:13418133-13418152 None:intergenic 40.0%
CAAAGGACATCGTATATAGG+CGG - chr2.2:13417657-13417676 MS.gene073146:intron 40.0%
GCTTTATGCATTGCGTTCTT+TGG - chr2.2:13416810-13416829 MS.gene073146:intron 40.0%
GGCTCCTTCTCTTATTATGA+TGG - chr2.2:13416643-13416662 MS.gene073146:CDS 40.0%
GTCATGCAGCTCAAAGTTAT+TGG + chr2.2:13416404-13416423 None:intergenic 40.0%
TACCAACTGAGTTAAGCTCA+CGG + chr2.2:13417563-13417582 None:intergenic 40.0%
! GATGGACATGTTTCAAGTAG+AGG - chr2.2:13417778-13417797 MS.gene073146:CDS 40.0%
! TGAGCTTAACTCAGTTGGTA+AGG - chr2.2:13417563-13417582 MS.gene073146:intron 40.0%
!! ACCTATCAGTTTTAGCCCTA+GGG + chr2.2:13416741-13416760 None:intergenic 40.0%
!! TGATCAAATGATGGGTTTGG+AGG + chr2.2:13416562-13416581 None:intergenic 40.0%
!! TGGTGATCAAATGATGGGTT+TGG + chr2.2:13416565-13416584 None:intergenic 40.0%
!!! ATCTTTTTTGGTGGTGTCCA+GGG + chr2.2:13417624-13417643 None:intergenic 40.0%
!!! TATCTTTTTTGGTGGTGTCC+AGG + chr2.2:13417625-13417644 None:intergenic 40.0%
ACCAACTGAGTTAAGCTCAC+GGG + chr2.2:13417562-13417581 None:intergenic 45.0%
ACCCTAGGGCTAAAACTGAT+AGG - chr2.2:13416737-13416756 MS.gene073146:CDS 45.0%
AGCTAGGAAGTTGAATGCTG+AGG - chr2.2:13417197-13417216 MS.gene073146:intron 45.0%
ATGACCTTCAAGCACAAGCT+AGG - chr2.2:13417181-13417200 MS.gene073146:intron 45.0%
ATTCGTCTTGTGCAGAGACT+TGG + chr2.2:13417520-13417539 None:intergenic 45.0%
CCAAGACTCTCATTTCTGAG+AGG - chr2.2:13416761-13416780 MS.gene073146:intron 45.0%
CCTCTCAGAAATGAGAGTCT+TGG + chr2.2:13416764-13416783 None:intergenic 45.0%
GAGAAGGAGCCAATTGTTGT+AGG + chr2.2:13416634-13416653 None:intergenic 45.0%
GATGGAGAGGTTAAAGGAGA+AGG - chr2.2:13416661-13416680 MS.gene073146:CDS 45.0%
GGAGAAGGAGAAAATGATGG+AGG - chr2.2:13416676-13416695 MS.gene073146:CDS 45.0%
! AATTTGAAGCTATGGGTGGC+TGG - chr2.2:13418095-13418114 MS.gene073146:CDS 45.0%
! ACTTCCTAGCTTGTGCTTGA+AGG + chr2.2:13417188-13417207 None:intergenic 45.0%
! AGGCCTTATCCATCTGTATG+TGG + chr2.2:13417131-13417150 None:intergenic 45.0%
! ATTTCTGAGAGGAGAAGGAG+AGG - chr2.2:13416772-13416791 MS.gene073146:intron 45.0%
! CTCTCATTTCTGAGAGGAGA+AGG - chr2.2:13416767-13416786 MS.gene073146:intron 45.0%
! GAAGGAGAAAATGATGGAGG+TGG - chr2.2:13416679-13416698 MS.gene073146:CDS 45.0%
!! ATCAGTTTTAGCCCTAGGGT+TGG + chr2.2:13416737-13416756 None:intergenic 45.0%
!! GACCTATCAGTTTTAGCCCT+AGG + chr2.2:13416742-13416761 None:intergenic 45.0%
!!! TAATTATAATTAGTTTTTAA+GGG + chr2.2:13416926-13416945 None:intergenic 5.0%
CCAACTGAGTTAAGCTCACG+GGG + chr2.2:13417561-13417580 None:intergenic 50.0%
GAGAAGGAGAGGAAGGATGA+AGG - chr2.2:13416783-13416802 MS.gene073146:intron 50.0%
GAGCCAATTGTTGTAGGGCT+TGG + chr2.2:13416628-13416647 None:intergenic 50.0%
GATCGATGATGCCAACCCTA+GGG - chr2.2:13416723-13416742 MS.gene073146:CDS 50.0%
GCTCACGGGGACTATCTTAT+TGG + chr2.2:13417548-13417567 None:intergenic 50.0%
! GCTGGTGCTCTTTTGAACCA+AGG - chr2.2:13418113-13418132 MS.gene073146:CDS 50.0%
CCCCGTGAGCTTAACTCAGT+TGG - chr2.2:13417558-13417577 MS.gene073146:intron 55.0%
CGATCGATGATGCCAACCCT+AGG - chr2.2:13416722-13416741 MS.gene073146:CDS 55.0%
CTGAGAGGAGAAGGAGAGGA+AGG - chr2.2:13416776-13416795 MS.gene073146:intron 55.0%
! AAGGTCTGGGGTTCGAACCC+TGG - chr2.2:13417604-13417623 MS.gene073146:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 13416382 13418160 13416382 ID=MS.gene073146
chr2.2 mRNA 13416382 13418160 13416382 ID=MS.gene073146.t1;Parent=MS.gene073146
chr2.2 exon 13417693 13418160 13417693 ID=MS.gene073146.t1.exon1;Parent=MS.gene073146.t1
chr2.2 CDS 13417693 13418160 13417693 ID=cds.MS.gene073146.t1;Parent=MS.gene073146.t1
chr2.2 exon 13417219 13417404 13417219 ID=MS.gene073146.t1.exon2;Parent=MS.gene073146.t1
chr2.2 CDS 13417219 13417404 13417219 ID=cds.MS.gene073146.t1;Parent=MS.gene073146.t1
chr2.2 exon 13416590 13416763 13416590 ID=MS.gene073146.t1.exon3;Parent=MS.gene073146.t1
chr2.2 CDS 13416590 13416763 13416590 ID=cds.MS.gene073146.t1;Parent=MS.gene073146.t1
chr2.2 exon 13416382 13416480 13416382 ID=MS.gene073146.t1.exon4;Parent=MS.gene073146.t1
chr2.2 CDS 13416382 13416480 13416382 ID=cds.MS.gene073146.t1;Parent=MS.gene073146.t1
Gene Sequence

>MS.gene073146

ATGGACTCACTAGAGAACACCAATAACTTTGAGCTGCATGACTTCATTGACGATCCAAACTTTGATCAATTTATCGATTTAATTCGAGGTGAACATGAAGATGCAATATCTAACTTTGGTTCTGACCTTATCAATGATTGCTTCATTGATAATAACATAAACCAACTTCTTTCAATTCCTCCAAACCCATCATTTGATCACCACAACAATATTATTAATAATAATCTTGTTAATGAGTATAATCCAAGCCCTACAACAATTGGCTCCTTCTCTTATTATGATGGAGAGGTTAAAGGAGAAGGAGAAAATGATGGAGGTGGTTCTTCTGCAACAACAACAACGATCGATGATGCCAACCCTAGGGCTAAAACTGATAGGTCCAAGACTCTCATTTCTGAGAGGAGAAGGAGAGGAAGGATGAAGGATAAGCTTTATGCATTGCGTTCTTTGGTTCCCAACATTACAAAGGTAATTAGTGTCCTTTATTTCGATTAATTTCGAACTCTAGAACTCTTGCTCTTGCATAAAACTCATGTAGTGTCCCTTAAAAACTAATTATAATTAGTGTCCCTTAAGTTGCAAATGTAAAGTTGCAAAGATATAATTTTGGTTAAACATTTATAGATTTTTTTTTTTTTTTTGCACTAGAGGTTAGGGTTGCTACTTTTAAGTTTTCAATATGATATGCTTGATATTAATTTGAGGATGAAAAGTGTAATGTAATTGATATACTTATATTTATATATCCACATACAGATGGATAAGGCCTCTATAATTGGAGATGCAGTATCATATGTGCATGACCTTCAAGCACAAGCTAGGAAGTTGAATGCTGAGGTTTCAGGACTTGAAGCATCTTTATCTGTGTCTGAAAATTATCAAGGATCAATTAGTAACACCATTAATGTTCAAAGCCATCCAATTTGCAAGAAAATTATCCAGGTTTGTTTAATTTTTCATTTCACATTCTAGTTTTTTTTTTTTTTTTTTTAGATCCAAGTTATAATTTCATGAGTCTATAAATCCATATAATAAAGAAAAAACTTTACAAGCATTTTTTTTTCTTTTACAAGACTCAAACTCTAAACCTTATCTTAAACCAACATTTTTTCTTAACGACAAAATTTATTAATAACCAAGTCTCTGCACAAGACGAATAGAAACCAATAAGATAGTCCCCGTGAGCTTAACTCAGTTGGTAAGGACAGTGCATAATATATGCAAGGTCTGGGGTTCGAACCCTGGACACCACCAAAAAAGATAGAAAATTACAAACAAAGGACATCGTATATAGGCGGAACACACAACAGAAAACTCGTTATCTTAGACCAACACTTTATAGTTGGTTTTTCAATGTATCGTACACAATTATAATAGTTGTGCATTATGCTATGTAGATGGACATGTTTCAAGTAGAGGAAAGAGGTTATTATGCAAAAATAATGTGCAATAAAGGAGAAGGAGTAGCTGCTTCATTGTACAAGGCTCTTGAGTCTCTTGCAAATTTCAATGTGCAAAATTCAAACTTAGCTACAGTTTGTGACACTTTTCTACTTACATTTACATTGAATGTAAGTTATTATTATTAGCCTCTCAATATCCAATTCATTGATTAATGATTAGGGTTATTGATGTTTTTGCTTAAGGATTAATTAGTCATTGATGTGTTGAAAAATATAGGTAAACGGTTCTGAACCAGAAATGAACCTTCAAAATTTGAAGCTATGGGTGGCTGGTGCTCTTTTGAACCAAGGCTTTGAATTCATGCCATCATTTTGA
Protein sequence

>MS.gene073146.t1

MDSLENTNNFELHDFIDDPNFDQFIDLIRGEHEDAISNFGSDLINDCFIDNNINQLLSIPPNPSFDHHNNIINNNLVNEYNPSPTTIGSFSYYDGEVKGEGENDGGGSSATTTTIDDANPRAKTDRSKTLISERRRRGRMKDKLYALRSLVPNITKMDKASIIGDAVSYVHDLQAQARKLNAEVSGLEASLSVSENYQGSISNTINVQSHPICKKIIQMDMFQVEERGYYAKIMCNKGEGVAASLYKALESLANFNVQNSNLATVCDTFLLTFTLNVNGSEPEMNLQNLKLWVAGALLNQGFEFMPSF