AlfalfaGEDB | MS.gene073231

M. sativa cultivar XinJiangDaYe / MS.gene073231

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073231.t1	AES87914.2	74.8	139	23	2	1	139	1	127	1.20E-43	186

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073231.t1	G7JSH2	74.8	139	23	2	1	139	1	127	8.3e-44	186.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050020	MS.gene073231	0.818854	1.58E-52	-1.69E-46
MS.gene050584	MS.gene073231	0.814451	1.53E-51	-1.69E-46
MS.gene050585	MS.gene073231	0.812233	4.70E-51	-1.69E-46
MS.gene050587	MS.gene073231	0.801944	7.09E-49	-1.69E-46
MS.gene051789	MS.gene073231	0.807717	4.41E-50	-1.69E-46
MS.gene052142	MS.gene073231	0.814101	1.83E-51	-1.69E-46
MS.gene053074	MS.gene073231	0.817498	3.20E-52	-1.69E-46
MS.gene05456	MS.gene073231	0.815229	1.03E-51	-1.69E-46
MS.gene055359	MS.gene073231	0.800129	1.67E-48	-1.69E-46
MS.gene059006	MS.gene073231	0.820994	5.11E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073231.t1	MTR_4g037480	82.014	139	13	2	1	139	1	127	1.49e-64	192
MS.gene073231.t1	MTR_4g037560	82.270	141	20	3	1	139	1	138	1.53e-59	180
MS.gene073231.t1	MTR_2g090185	51.079	139	55	5	1	139	1	126	3.24e-27	98.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073231.t1	AT2G20870	41.414	99	53	4	35	130	33	129	1.00e-11	58.9

Find 47 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCTAATAATCCATTTAT+TGG	0.205431	4.3:-54947506	MS.gene073231:CDS
TACCCTGGCATTGGACATTT+AGG	0.235381	4.3:-54947551	MS.gene073231:CDS
CCTCCAGTGCCACCAATAAA+TGG	0.254443	4.3:+54947494	None:intergenic
GGAGGATCAGGTTCGGGTTT+AGG	0.265984	4.3:-54947476	MS.gene073231:CDS
CCACCACCATTGCCGGGATT+TGG	0.266687	4.3:+54947362	None:intergenic
CCACCAATAAATGGATTATT+AGG	0.286771	4.3:+54947503	None:intergenic
GGCACTGGAGGATCAGGTTC+GGG	0.362114	4.3:-54947482	MS.gene073231:CDS
TGTTCCAAACCCTGGCTTTG+AGG	0.364651	4.3:-54947387	MS.gene073231:CDS
GGAACCTCAAAGCCAGGGTT+TGG	0.367827	4.3:+54947383	None:intergenic
TTAGGATTTCCACCGAAAAT+CGG	0.378656	4.3:-54947533	MS.gene073231:CDS
TGGCACTGGAGGATCAGGTT+CGG	0.387534	4.3:-54947483	MS.gene073231:CDS
CCCGGCAATGGTGGTGGCGG+CGG	0.389210	4.3:-54947356	MS.gene073231:CDS
ATTGGTGGCACTGGAGGATC+AGG	0.397280	4.3:-54947488	MS.gene073231:CDS
GGCAATGGTGGTGGCGGCGG+TGG	0.404461	4.3:-54947353	MS.gene073231:CDS
CTCTTTCTTGTCGTCGCTGT+CGG	0.412216	4.3:+54947613	None:intergenic
GGCTTTGAGGTTCCAAATCC+CGG	0.413720	4.3:-54947374	MS.gene073231:CDS
ACCGCCGCCACCACCATTGC+CGG	0.428039	4.3:+54947355	None:intergenic
TCAGGTTCGGGTTTAGGATC+AGG	0.431514	4.3:-54947470	MS.gene073231:CDS
AATCCATTTATTGGTGGCAC+TGG	0.434316	4.3:-54947497	MS.gene073231:CDS
TCAGGTCATAGTTATGTTCC+CGG	0.442709	4.3:-54947425	MS.gene073231:CDS
TAGGATTTCCACCGAAAATC+GGG	0.446688	4.3:-54947532	MS.gene073231:CDS
CCAAATCCCGGCAATGGTGG+TGG	0.459772	4.3:-54947362	MS.gene073231:CDS
TCAGGAACAGGAGCAGGATC+AGG	0.471562	4.3:-54947452	MS.gene073231:CDS
GGAGCAGGATCAGGATCATC+AGG	0.472228	4.3:-54947443	MS.gene073231:CDS
GGATTTGGAACCTCAAAGCC+AGG	0.493115	4.3:+54947377	None:intergenic
CCTAATAATCCATTTATTGG+TGG	0.494125	4.3:-54947503	MS.gene073231:CDS
ACATCACAGCAAGCAGTTGC+TGG	0.494474	4.3:-54947662	MS.gene073231:CDS
AACAAATGTGTCATCGCCAC+CGG	0.495697	4.3:+54947406	None:intergenic
CCATTTATTGGTGGCACTGG+AGG	0.501560	4.3:-54947494	MS.gene073231:CDS
AATCCTAAATGTCCAATGCC+AGG	0.501871	4.3:+54947548	None:intergenic
TCGGGTTTAGGATCAGGAAC+AGG	0.510472	4.3:-54947464	MS.gene073231:CDS
AAACTACATTACCCTGGCAT+TGG	0.520118	4.3:-54947560	MS.gene073231:CDS
AATCCCGGCAATGGTGGTGG+CGG	0.520657	4.3:-54947359	MS.gene073231:CDS
CCGCCGCCACCACCATTGCC+GGG	0.521155	4.3:+54947356	None:intergenic
CGGTGGTAGAGTTGCATCAA+CGG	0.529347	4.3:-54947336	MS.gene073231:CDS
GAGGTTCCAAATCCCGGCAA+TGG	0.537778	4.3:-54947368	MS.gene073231:CDS
TTAGGATCAGGAACAGGAGC+AGG	0.546096	4.3:-54947458	MS.gene073231:CDS
GACACATTTGTTCCAAACCC+TGG	0.566936	4.3:-54947395	MS.gene073231:CDS
ACAAATGTGTCATCGCCACC+GGG	0.594099	4.3:+54947407	None:intergenic
CTGCTTGCTGTGATGTGACA+AGG	0.599798	4.3:+54947669	None:intergenic
GTTCCAAATCCCGGCAATGG+TGG	0.615486	4.3:-54947365	MS.gene073231:CDS
ATCCTAAATGTCCAATGCCA+GGG	0.617878	4.3:+54947549	None:intergenic
TCTTAGCATTAGCAATGCTG+CGG	0.631738	4.3:+54947639	None:intergenic
GATTTGGAACCTCAAAGCCA+GGG	0.635813	4.3:+54947378	None:intergenic
CATGGTAAACTACATTACCC+TGG	0.662493	4.3:-54947566	MS.gene073231:CDS
GGTCATAGTTATGTTCCCGG+TGG	0.737137	4.3:-54947422	MS.gene073231:CDS
CTTGCTGTGATGTGACAAGG+AGG	0.757626	4.3:+54947672	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACTCCTAATAATCCATTTAT+TGG	-	chr4.3:54947541-54947560	MS.gene073231:CDS	25.0%
!!	TTTTTATTCAAGCATGATCA+TGG	-	chr4.3:54947463-54947482	MS.gene073231:CDS	25.0%
	CCTAATAATCCATTTATTGG+TGG	-	chr4.3:54947544-54947563	MS.gene073231:CDS	30.0%
	TCATGCTTGAATAAAAACTG+CGG	+	chr4.3:54947460-54947479	None:intergenic	30.0%
!!	CCACCAATAAATGGATTATT+AGG	+	chr4.3:54947547-54947566	None:intergenic	30.0%
	TTAGGATTTCCACCGAAAAT+CGG	-	chr4.3:54947514-54947533	MS.gene073231:CDS	35.0%
	AAACTACATTACCCTGGCAT+TGG	-	chr4.3:54947487-54947506	MS.gene073231:CDS	40.0%
	AATCCTAAATGTCCAATGCC+AGG	+	chr4.3:54947502-54947521	None:intergenic	40.0%
	ATCCTAAATGTCCAATGCCA+GGG	+	chr4.3:54947501-54947520	None:intergenic	40.0%
	CATGGTAAACTACATTACCC+TGG	-	chr4.3:54947481-54947500	MS.gene073231:CDS	40.0%
	TAGGATTTCCACCGAAAATC+GGG	-	chr4.3:54947515-54947534	MS.gene073231:CDS	40.0%
	TCAGGTCATAGTTATGTTCC+CGG	-	chr4.3:54947622-54947641	MS.gene073231:CDS	40.0%
	TCTTAGCATTAGCAATGCTG+CGG	+	chr4.3:54947411-54947430	None:intergenic	40.0%
!	AATCCATTTATTGGTGGCAC+TGG	-	chr4.3:54947550-54947569	MS.gene073231:CDS	40.0%
!	TTAGGAGTAAGCCCGATTTT+CGG	+	chr4.3:54947529-54947548	None:intergenic	40.0%
	AACAAATGTGTCATCGCCAC+CGG	+	chr4.3:54947644-54947663	None:intergenic	45.0%
	GACACATTTGTTCCAAACCC+TGG	-	chr4.3:54947652-54947671	MS.gene073231:CDS	45.0%
!	GATTTGGAACCTCAAAGCCA+GGG	+	chr4.3:54947672-54947691	None:intergenic	45.0%
!	TACCCTGGCATTGGACATTT+AGG	-	chr4.3:54947496-54947515	MS.gene073231:CDS	45.0%
	ACAAATGTGTCATCGCCACC+GGG	+	chr4.3:54947643-54947662	None:intergenic	50.0%
	ACATCACAGCAAGCAGTTGC+TGG	-	chr4.3:54947385-54947404	MS.gene073231:CDS	50.0%
	CCTCCAGTGCCACCAATAAA+TGG	+	chr4.3:54947556-54947575	None:intergenic	50.0%
	CGGTGGTAGAGTTGCATCAA+CGG	-	chr4.3:54947711-54947730	MS.gene073231:CDS	50.0%
	CTCTTTCTTGTCGTCGCTGT+CGG	+	chr4.3:54947437-54947456	None:intergenic	50.0%
	GGTCATAGTTATGTTCCCGG+TGG	-	chr4.3:54947625-54947644	MS.gene073231:CDS	50.0%
	TTAGGATCAGGAACAGGAGC+AGG	-	chr4.3:54947589-54947608	MS.gene073231:CDS	50.0%
!	CCATTTATTGGTGGCACTGG+AGG	-	chr4.3:54947553-54947572	MS.gene073231:CDS	50.0%
!	CTTGCTGTGATGTGACAAGG+AGG	+	chr4.3:54947378-54947397	None:intergenic	50.0%
!	GGATTTGGAACCTCAAAGCC+AGG	+	chr4.3:54947673-54947692	None:intergenic	50.0%
!	TCAGGTTCGGGTTTAGGATC+AGG	-	chr4.3:54947577-54947596	MS.gene073231:CDS	50.0%
!	TCGGGTTTAGGATCAGGAAC+AGG	-	chr4.3:54947583-54947602	MS.gene073231:CDS	50.0%
!!	CTGCTTGCTGTGATGTGACA+AGG	+	chr4.3:54947381-54947400	None:intergenic	50.0%
!!	GGCTTTGAGGTTCCAAATCC+CGG	-	chr4.3:54947673-54947692	MS.gene073231:CDS	50.0%
!!	TGTTCCAAACCCTGGCTTTG+AGG	-	chr4.3:54947660-54947679	MS.gene073231:CDS	50.0%
	GAGGTTCCAAATCCCGGCAA+TGG	-	chr4.3:54947679-54947698	MS.gene073231:CDS	55.0%
	GGAACCTCAAAGCCAGGGTT+TGG	+	chr4.3:54947667-54947686	None:intergenic	55.0%
	GGAGCAGGATCAGGATCATC+AGG	-	chr4.3:54947604-54947623	MS.gene073231:CDS	55.0%
	GTTCCAAATCCCGGCAATGG+TGG	-	chr4.3:54947682-54947701	MS.gene073231:CDS	55.0%
	TCAGGAACAGGAGCAGGATC+AGG	-	chr4.3:54947595-54947614	MS.gene073231:CDS	55.0%
!	ATTGGTGGCACTGGAGGATC+AGG	-	chr4.3:54947559-54947578	MS.gene073231:CDS	55.0%
!	GGAGGATCAGGTTCGGGTTT+AGG	-	chr4.3:54947571-54947590	MS.gene073231:CDS	55.0%
!	GGAGTAAGCCCGATTTTCGG+TGG	+	chr4.3:54947526-54947545	None:intergenic	55.0%
!	TGGCACTGGAGGATCAGGTT+CGG	-	chr4.3:54947564-54947583	MS.gene073231:CDS	55.0%
	CCAAATCCCGGCAATGGTGG+TGG	-	chr4.3:54947685-54947704	MS.gene073231:CDS	60.0%
	CCACCACCATTGCCGGGATT+TGG	+	chr4.3:54947688-54947707	None:intergenic	60.0%
!	GGCACTGGAGGATCAGGTTC+GGG	-	chr4.3:54947565-54947584	MS.gene073231:CDS	60.0%
!!	AATCCCGGCAATGGTGGTGG+CGG	-	chr4.3:54947688-54947707	MS.gene073231:CDS	60.0%
	ACCGCCGCCACCACCATTGC+CGG	+	chr4.3:54947695-54947714	None:intergenic	70.0%
	CCGCCGCCACCACCATTGCC+GGG	+	chr4.3:54947694-54947713	None:intergenic	75.0%
!!	CCCGGCAATGGTGGTGGCGG+CGG	-	chr4.3:54947691-54947710	MS.gene073231:CDS	75.0%
!!	GGCAATGGTGGTGGCGGCGG+TGG	-	chr4.3:54947694-54947713	MS.gene073231:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	54947325	54947744	54947325	ID=MS.gene073231
chr4.3	mRNA	54947325	54947744	54947325	ID=MS.gene073231.t1;Parent=MS.gene073231
chr4.3	exon	54947325	54947744	54947325	ID=MS.gene073231.t1.exon1;Parent=MS.gene073231.t1
chr4.3	CDS	54947325	54947744	54947325	ID=cds.MS.gene073231.t1;Parent=MS.gene073231.t1

Gene Sequence

>MS.gene073231

ATGGTTCAAAGAGCATACTCTTTCTTTGCACTTTTTCTCATTTTCAATATCCTCCTTGTCACATCACAGCAAGCAGTTGCTGGCCGCAGCATTGCTAATGCTAAGAACTCCGACAGCGACGACAAGAAAGAGCCGCAGTTTTTATTCAAGCATGATCATGGTAAACTACATTACCCTGGCATTGGACATTTAGGATTTCCACCGAAAATCGGGCTTACTCCTAATAATCCATTTATTGGTGGCACTGGAGGATCAGGTTCGGGTTTAGGATCAGGAACAGGAGCAGGATCAGGATCATCAGGTCATAGTTATGTTCCCGGTGGCGATGACACATTTGTTCCAAACCCTGGCTTTGAGGTTCCAAATCCCGGCAATGGTGGTGGCGGCGGTGGTAGAGTTGCATCAACGGTTCATCCATGA

Protein sequence

>MS.gene073231.t1

MVQRAYSFFALFLIFNILLVTSQQAVAGRSIANAKNSDSDDKKEPQFLFKHDHGKLHYPGIGHLGFPPKIGLTPNNPFIGGTGGSGSGLGSGTGAGSGSSGHSYVPGGDDTFVPNPGFEVPNPGNGGGGGGRVASTVHP