AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073414

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073414.t1 MTR_2g437700 98.417 379 5 1 1 379 12 389 0.0 735
MS.gene073414.t1 MTR_7g075453 32.759 348 214 11 16 361 23 352 8.07e-37 138
MS.gene073414.t1 MTR_2g018340 41.558 154 84 3 182 332 37 187 1.30e-31 119
MS.gene073414.t1 MTR_3g460780 28.840 319 213 10 16 333 31 336 9.44e-26 107
MS.gene073414.t1 MTR_5g098420 27.692 325 215 11 12 333 31 338 5.94e-22 97.1
MS.gene073414.t1 MTR_7g101080 31.677 161 101 4 179 335 35 190 4.63e-17 80.5
MS.gene073414.t1 MTR_2g018380 36.154 130 72 4 230 353 53 177 6.65e-17 79.0
MS.gene073414.t1 MTR_5g098060 34.615 182 106 5 182 360 46 217 8.23e-17 80.1
MS.gene073414.t1 MTR_4g059840 32.787 183 109 7 183 360 72 245 7.78e-15 74.7
MS.gene073414.t1 MTR_2g093980 29.221 154 102 3 184 334 69 218 2.50e-14 73.2
MS.gene073414.t1 MTR_2g017950 30.189 159 98 4 183 332 70 224 4.42e-14 72.4
MS.gene073414.t1 MTR_8g087460 30.769 156 97 4 183 332 92 242 4.74e-14 72.8
MS.gene073414.t1 MTR_8g087470 30.769 156 97 4 183 332 92 242 5.71e-14 72.4
MS.gene073414.t1 MTR_4g059790 30.387 181 116 5 183 360 72 245 5.73e-14 72.0
MS.gene073414.t1 MTR_4g053380 31.111 180 111 6 183 355 76 249 1.31e-13 71.2
MS.gene073414.t1 MTR_2g090765 29.730 185 121 5 153 334 8 186 8.48e-13 68.2
MS.gene073414.t1 MTR_8g087450 31.544 149 95 4 184 329 77 221 1.28e-12 68.2
MS.gene073414.t1 MTR_2g093970 29.139 151 100 4 184 331 69 215 3.87e-12 66.6
MS.gene073414.t1 MTR_4g059720 27.778 162 108 4 174 333 35 189 4.04e-12 66.2
MS.gene073414.t1 MTR_2g017970 29.213 178 117 6 184 358 68 239 6.64e-12 65.9
MS.gene073414.t1 MTR_2g017960 29.213 178 117 6 184 358 68 239 1.02e-11 65.5
MS.gene073414.t1 MTR_2g093990 28.947 152 102 3 184 333 41 188 1.19e-11 64.7
MS.gene073414.t1 MTR_1g009950 28.161 174 114 5 14 184 23 188 2.79e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073414.t1 AT3G46550 55.202 346 137 5 15 346 29 370 2.62e-119 352
MS.gene073414.t1 AT2G45470 33.333 324 202 10 14 336 25 335 2.23e-35 134
MS.gene073414.t1 AT3G60900 32.471 348 213 11 16 354 27 361 8.17e-31 122
MS.gene073414.t1 AT5G55730 29.538 325 213 11 10 333 20 329 8.55e-26 108
MS.gene073414.t1 AT5G55730 29.538 325 213 11 10 333 20 329 8.55e-26 108
MS.gene073414.t1 AT4G12730 28.358 335 223 12 1 333 13 332 5.77e-24 102
MS.gene073414.t1 AT5G44130 29.798 198 132 5 183 378 35 227 7.06e-18 82.8
MS.gene073414.t1 AT5G60490 31.579 152 99 3 184 333 39 187 3.06e-17 80.9
MS.gene073414.t1 AT1G03870 29.282 181 119 4 183 359 38 213 4.12e-15 74.7
MS.gene073414.t1 AT2G20520 31.646 158 97 5 183 334 37 189 3.45e-14 72.0
MS.gene073414.t1 AT5G03170 33.962 159 99 4 179 334 31 186 1.49e-13 70.5
MS.gene073414.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.54e-13 69.7
MS.gene073414.t1 AT2G04780 30.380 158 99 4 183 336 45 195 2.54e-13 69.7
MS.gene073414.t1 AT3G12660 31.210 157 103 3 10 166 19 170 5.34e-13 68.9

Find 148 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTAATGGTGTTGGTGTTC+GGG 0.251448 2.4:+54123411 None:intergenic
GATTAACACTGGCATTGTTC+AGG 0.317584 2.4:-54122889 MS.gene073414:CDS
TTGTTGCCAAGAGAGATATT+TGG 0.326646 2.4:-54122804 MS.gene073414:CDS
GAGGTGAATGTTGATAATTC+AGG 0.334884 2.4:+54123710 None:intergenic
CTTTCTTCACCGCCAGGGTT+TGG 0.356296 2.4:-54122675 MS.gene073414:CDS
GCTTCAATGCTCTCCGCTTC+CGG 0.361932 2.4:-54123188 MS.gene073414:CDS
GTGAGGGTCGGTGGTGAGAT+AGG 0.366350 2.4:+54123610 None:intergenic
TGTTGCCAAGAGAGATATTT+GGG 0.370591 2.4:-54122803 MS.gene073414:CDS
ATGGTGTTGGGGATAGGATT+AGG 0.379163 2.4:+54123771 None:intergenic
GTGAGCATCGATGAGGGTTT+TGG 0.379755 2.4:+54123226 None:intergenic
GGTCGGACCAGGAGAGGAAC+TGG 0.383020 2.4:+54123531 None:intergenic
ATTGTAATGGTGTTGGTGTT+CGG 0.388000 2.4:+54123410 None:intergenic
TGGTGTTGGTGTTCGGGGAG+TGG 0.394617 2.4:+54123417 None:intergenic
GATGTCAGCGAGAGCGGAGG+GGG 0.395262 2.4:+54123574 None:intergenic
TGGTGGCCCGGCCGGTGGTT+TGG 0.397807 2.4:+54123465 None:intergenic
TCCTCCTCCAGTTCCTCTCC+TGG 0.402978 2.4:-54123538 MS.gene073414:CDS
GGTGTTGGTGTTCGGGGAGT+GGG 0.411168 2.4:+54123418 None:intergenic
TGAAAGAGAAAGATGGTGTT+GGG 0.411336 2.4:+54123759 None:intergenic
GGAGATTTCGCAGACACAAT+TGG 0.413692 2.4:+54122774 None:intergenic
CTTCGTGCTTATTACCCTCT+CGG 0.420188 2.4:-54123017 MS.gene073414:CDS
GGCCGGGCCACCAACAACTT+TGG 0.425079 2.4:-54123455 MS.gene073414:CDS
GGGGATAAACAAGGTAATTC+CGG 0.425532 2.4:+54123130 None:intergenic
GTCGGTGGTGAGATAGGTGT+TGG 0.428158 2.4:+54123616 None:intergenic
AAGAGTAAGGTGCTGGGGAA+TGG 0.428506 2.4:+54123387 None:intergenic
GAAGCCGGTGAAGGTGGTTC+CGG 0.429193 2.4:-54123149 MS.gene073414:CDS
GGGGAGAGGTTATGGAGGTG+AGG 0.434679 2.4:+54123593 None:intergenic
TTCTTCCCAAATATCTCTCT+TGG 0.439416 2.4:+54122798 None:intergenic
TGAAAGAACGGTGGCGTTTG+AGG 0.443446 2.4:+54123364 None:intergenic
GAGGTGTTGGAGGTCGGACC+AGG 0.448627 2.4:+54123520 None:intergenic
AGAAAGATGGTGTTGGGGAT+AGG 0.453694 2.4:+54123765 None:intergenic
GAGAGTGGTGACGAGTTTAC+CGG 0.456170 2.4:+54123490 None:intergenic
AGAGGAACTGGAGGAGGATG+TGG 0.458663 2.4:+54123543 None:intergenic
GACGCTTGCGACGGAAGCTA+TGG 0.462272 2.4:-54122961 MS.gene073414:CDS
CGGAGGGAGGGAGGTGTTGG+AGG 0.464496 2.4:+54123510 None:intergenic
CCCTTACGGTTTCGATCTCA+TGG 0.465462 2.4:-54123285 MS.gene073414:CDS
GATGCTGCTTCTTCGCCGGC+GGG 0.465731 2.4:-54122720 MS.gene073414:CDS
ATTAACACTGGCATTGTTCA+GGG 0.469145 2.4:-54122888 MS.gene073414:CDS
GGTGAAGATAGTGAGATTGT+AGG 0.469199 2.4:+54123325 None:intergenic
TCACATCTTTCTTCACCGCC+AGG 0.471478 2.4:-54122681 MS.gene073414:CDS
CTCTCTTCCAAACCACCGGC+CGG 0.475597 2.4:-54123472 MS.gene073414:CDS
TTCAGGGAAGGTTGAAAGGA+GGG 0.476637 2.4:+54123727 None:intergenic
AGGTGAATGTTGATAATTCA+GGG 0.477819 2.4:+54123711 None:intergenic
AGCCAAAGTTGTTGGTGGCC+CGG 0.478504 2.4:+54123453 None:intergenic
TAGTGCTCCTCCTCCTTATG+AGG 0.479474 2.4:-54122742 MS.gene073414:CDS
GGGAGAGATCAGCGGTGAGA+GGG 0.486859 2.4:+54123669 None:intergenic
GAAACACCGAAAATCGCAAT+AGG 0.488328 2.4:+54122834 None:intergenic
TCGGTGGTGAGATAGGTGTT+GGG 0.492560 2.4:+54123617 None:intergenic
TTCATTCACAACGCCGGAAG+CGG 0.494673 2.4:+54123175 None:intergenic
TTTGAGGAAGAGTAAGGTGC+TGG 0.494680 2.4:+54123380 None:intergenic
AAAGTTGTTGGTGGCCCGGC+CGG 0.495578 2.4:+54123457 None:intergenic
TACCGGAGGGAGGGAGGTGT+TGG 0.497410 2.4:+54123507 None:intergenic
CGGGGAGTGGGTGATGTTGA+CGG 0.497837 2.4:+54123430 None:intergenic
AATTCCGGAACCACCTTCAC+CGG 0.498505 2.4:+54123145 None:intergenic
GAAGAAGCAGCATCCTCATA+AGG 0.500145 2.4:+54122729 None:intergenic
GTTGTTGGTGGCCCGGCCGG+TGG 0.501570 2.4:+54123460 None:intergenic
TCGGCGAAAGCATCGTCGAC+GGG 0.505539 2.4:+54123110 None:intergenic
AGGATGTCAGCGAGAGCGGA+GGG 0.508490 2.4:+54123572 None:intergenic
GTCGCAAGCGTCGGTTGCAA+CGG 0.511457 2.4:+54122972 None:intergenic
GCGACGGAAGCTATGGGTGC+TGG 0.514984 2.4:-54122954 MS.gene073414:CDS
AGGTTGAAAGGAGGGAGGTG+AGG 0.516160 2.4:+54123735 None:intergenic
ATAATTCAGGGAAGGTTGAA+AGG 0.519692 2.4:+54123723 None:intergenic
CTCCAACACCTCCCTCCCTC+CGG 0.523989 2.4:-54123509 MS.gene073414:CDS
GGAGGAGGAGCACTACTGTC+CGG 0.524608 2.4:+54122750 None:intergenic
TTGAAAGAGAAAGATGGTGT+TGG 0.524791 2.4:+54123758 None:intergenic
CACATCTTTCTTCACCGCCA+GGG 0.525421 2.4:-54122680 MS.gene073414:CDS
GTTATGGAGGTGAGGGTCGG+TGG 0.527291 2.4:+54123601 None:intergenic
GTGAAGATAGTGAGATTGTA+GGG 0.528446 2.4:+54123326 None:intergenic
GGGAGGGAGGTGTTGGAGGT+CGG 0.529487 2.4:+54123514 None:intergenic
ATTCAGGGAAGGTTGAAAGG+AGG 0.530201 2.4:+54123726 None:intergenic
TGGGAGAGATCAGCGGTGAG+AGG 0.533298 2.4:+54123668 None:intergenic
TCTCTTCCAAACCACCGGCC+GGG 0.536880 2.4:-54123471 MS.gene073414:CDS
GCGGAGGATGTCAGCGAGAG+CGG 0.537341 2.4:+54123568 None:intergenic
ACTGGAGGAGGATGTGGTAG+CGG 0.540375 2.4:+54123549 None:intergenic
GGAGAGAGAGTGAATTGCGG+TGG 0.541485 2.4:+54123648 None:intergenic
GGAATGGATTGTAATGGTGT+TGG 0.543280 2.4:+54123403 None:intergenic
GTTGACGGAGCCAAAGTTGT+TGG 0.548182 2.4:+54123445 None:intergenic
GAGGATGTCAGCGAGAGCGG+AGG 0.550692 2.4:+54123571 None:intergenic
GGGAGAGGTTATGGAGGTGA+GGG 0.552715 2.4:+54123594 None:intergenic
TGCTGGGGAATGGATTGTAA+TGG 0.553637 2.4:+54123397 None:intergenic
GGATGCTGCTTCTTCGCCGG+CGG 0.553952 2.4:-54122721 MS.gene073414:CDS
TGTAATGGTGTTGGTGTTCG+GGG 0.554234 2.4:+54123412 None:intergenic
ACCAGGAGAGGAACTGGAGG+AGG 0.555679 2.4:+54123537 None:intergenic
CGGTTTCGATCTCATGGCAT+CGG 0.555806 2.4:-54123279 MS.gene073414:CDS
GTGATGTTGAGAAGAATGCT+TGG 0.555902 2.4:+54123248 None:intergenic
GCCATGAGATCGAAACCGTA+AGG 0.558245 2.4:+54123284 None:intergenic
GTGGCGTTTGAGGAAGAGTA+AGG 0.559294 2.4:+54123374 None:intergenic
GAGGTTATGGAGGTGAGGGT+CGG 0.559779 2.4:+54123598 None:intergenic
GTGAGGTTGAAAGAGAAAGA+TGG 0.560128 2.4:+54123752 None:intergenic
TGAGGATGCTGCTTCTTCGC+CGG 0.566290 2.4:-54122724 MS.gene073414:CDS
CGAAACGGAAGGAGGTAGAT+CGG 0.567781 2.4:+54123091 None:intergenic
TTGAGGAAGAGTAAGGTGCT+GGG 0.571039 2.4:+54123381 None:intergenic
GAGAGATCAGCGGTGAGAGG+GGG 0.573409 2.4:+54123671 None:intergenic
GGAGAGATCAGCGGTGAGAG+GGG 0.577869 2.4:+54123670 None:intergenic
GAATTCGAAGCCGGTGAAGG+TGG 0.580678 2.4:-54123155 MS.gene073414:CDS
GACGGAGCCAAAGTTGTTGG+TGG 0.580994 2.4:+54123448 None:intergenic
GGCTCTGTTGCGATTAACAC+TGG 0.583502 2.4:-54122900 MS.gene073414:CDS
CGGACCAGGAGAGGAACTGG+AGG 0.586171 2.4:+54123534 None:intergenic
ATAGCTTCCGTCGCAAGCGT+CGG 0.587413 2.4:+54122963 None:intergenic
GAGAGAGAGTGAATTGCGGT+GGG 0.591977 2.4:+54123649 None:intergenic
ATCGGCGAAAGCATCGTCGA+CGG 0.592319 2.4:+54123109 None:intergenic
ACGCTTGCGACGGAAGCTAT+GGG 0.595227 2.4:-54122960 MS.gene073414:CDS
TTCGAATTCATTCACAACGC+CGG 0.596662 2.4:+54123169 None:intergenic
GGGAAGTGATTGAAGCGAAA+CGG 0.597351 2.4:+54123076 None:intergenic
ACCACTCTCTTCCAAACCAC+CGG 0.598222 2.4:-54123476 MS.gene073414:CDS
AGGGATTAAAAGAGAATCAA+CGG 0.602507 2.4:+54123304 None:intergenic
GAAATTGTGAGCATCGATGA+GGG 0.603493 2.4:+54123220 None:intergenic
GAATGTTGATAATTCAGGGA+AGG 0.603611 2.4:+54123715 None:intergenic
GGTGAGATAGGTGTTGGGGA+CGG 0.604958 2.4:+54123622 None:intergenic
TGACGAGTTTACCGGAGGGA+GGG 0.605757 2.4:+54123498 None:intergenic
GCAGCATCCTCATAAGGAGG+AGG 0.606713 2.4:+54122735 None:intergenic
ATCGTCGACGGGGATAAACA+AGG 0.609796 2.4:+54123121 None:intergenic
TGAAATTGTGAGCATCGATG+AGG 0.610224 2.4:+54123219 None:intergenic
GGATGTCAGCGAGAGCGGAG+GGG 0.610527 2.4:+54123573 None:intergenic
CGAGTTTACCGGAGGGAGGG+AGG 0.611461 2.4:+54123501 None:intergenic
AGTGATTGAAGCGAAACGGA+AGG 0.612745 2.4:+54123080 None:intergenic
TGTGTCTGCGAAATCTCCAC+CGG 0.616503 2.4:-54122769 MS.gene073414:CDS
CGGTTGCAACGGATTCGCAG+TGG 0.617668 2.4:+54122983 None:intergenic
GTTGCAACCGACGCTTGCGA+CGG 0.617749 2.4:-54122970 MS.gene073414:CDS
AGGGAAGGTTGAAAGGAGGG+AGG 0.618290 2.4:+54123730 None:intergenic
CGGTGGTGAGATAGGTGTTG+GGG 0.618735 2.4:+54123618 None:intergenic
GGCTGTACATCAAAACCCGC+CGG 0.624496 2.4:+54122705 None:intergenic
GCCGGTGGTTTGGAAGAGAG+TGG 0.626248 2.4:+54123475 None:intergenic
TGGATTGAAGCGAACCGAGA+GGG 0.627636 2.4:+54123003 None:intergenic
TTAACACTGGCATTGTTCAG+GGG 0.631498 2.4:-54122887 MS.gene073414:CDS
GGAGGAGGATGTGGTAGCGG+AGG 0.631708 2.4:+54123552 None:intergenic
GTGACGAGTTTACCGGAGGG+AGG 0.632618 2.4:+54123497 None:intergenic
CCATGAGATCGAAACCGTAA+GGG 0.633243 2.4:+54123285 None:intergenic
AATGAATTCGAAGCCGGTGA+AGG 0.633955 2.4:-54123158 MS.gene073414:CDS
GTTGTGAATGAATTCGAAGC+CGG 0.634796 2.4:-54123164 MS.gene073414:CDS
GAAAGAGAAAGATGGTGTTG+GGG 0.638474 2.4:+54123760 None:intergenic
AGTGGTGACGAGTTTACCGG+AGG 0.642661 2.4:+54123493 None:intergenic
GGGTGTGGAAGAAAGTAGGG+AGG 0.644752 2.4:+54123691 None:intergenic
GTTGGAGGTCGGACCAGGAG+AGG 0.645093 2.4:+54123525 None:intergenic
CGGCGAAAGCATCGTCGACG+GGG 0.646874 2.4:+54123111 None:intergenic
CTAACATCTTCACCAAACCC+TGG 0.655235 2.4:+54122663 None:intergenic
TGAGGAAGAGTAAGGTGCTG+GGG 0.655459 2.4:+54123382 None:intergenic
GGAGGAGCACTACTGTCCGG+TGG 0.656903 2.4:+54122753 None:intergenic
GGCGGTGAAGAAAGATGTGA+CGG 0.657561 2.4:+54122684 None:intergenic
GAAGCAGCATCCTCATAAGG+AGG 0.663333 2.4:+54122732 None:intergenic
GTGGTGACGAGTTTACCGGA+GGG 0.663417 2.4:+54123494 None:intergenic
GATAGGTGTTGGGGACGGCG+AGG 0.663521 2.4:+54123627 None:intergenic
GATTGAAGCGAAACGGAAGG+AGG 0.667934 2.4:+54123083 None:intergenic
AACATGCGCTTTGAGAACAA+CGG 0.685880 2.4:+54123040 None:intergenic
CGAGGAGAGAGAGTGAATTG+CGG 0.703819 2.4:+54123645 None:intergenic
GTGGATTGAAGCGAACCGAG+AGG 0.709717 2.4:+54123002 None:intergenic
ATTGCGGTGGGAGAGATCAG+CGG 0.710115 2.4:+54123661 None:intergenic
TTTGAGCTGTGAAAGAACGG+TGG 0.719224 2.4:+54123355 None:intergenic
ACATCTTCACCAAACCCTGG+CGG 0.727699 2.4:+54122666 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAATATTTCAACTTTTAA+TGG - chr2.4:54123438-54123457 MS.gene073414:CDS 15.0%
AGGGATTAAAAGAGAATCAA+CGG + chr2.4:54123058-54123077 None:intergenic 30.0%
! GATTCTCTTTTAATCCCTTA+CGG - chr2.4:54123060-54123079 MS.gene073414:CDS 30.0%
!! AGGTGAATGTTGATAATTCA+GGG + chr2.4:54122651-54122670 None:intergenic 30.0%
ATAATTCAGGGAAGGTTGAA+AGG + chr2.4:54122639-54122658 None:intergenic 35.0%
ATTAACACTGGCATTGTTCA+GGG - chr2.4:54123471-54123490 MS.gene073414:CDS 35.0%
GTGAAGATAGTGAGATTGTA+GGG + chr2.4:54123036-54123055 None:intergenic 35.0%
TCAGATGTTGCAAGTGTTTA+TGG - chr2.4:54123720-54123739 MS.gene073414:CDS 35.0%
TGAAAGAGAAAGATGGTGTT+GGG + chr2.4:54122603-54122622 None:intergenic 35.0%
TGTTGCCAAGAGAGATATTT+GGG - chr2.4:54123556-54123575 MS.gene073414:CDS 35.0%
TTCTTCCCAAATATCTCTCT+TGG + chr2.4:54123564-54123583 None:intergenic 35.0%
TTGAAAGAGAAAGATGGTGT+TGG + chr2.4:54122604-54122623 None:intergenic 35.0%
TTGTTGCCAAGAGAGATATT+TGG - chr2.4:54123555-54123574 MS.gene073414:CDS 35.0%
! CAGAATCCTATTGCGATTTT+CGG - chr2.4:54123519-54123538 MS.gene073414:CDS 35.0%
! CATTTTGAGCTGTGAAAGAA+CGG + chr2.4:54123010-54123029 None:intergenic 35.0%
!! ATTGTAATGGTGTTGGTGTT+CGG + chr2.4:54122952-54122971 None:intergenic 35.0%
!! GAATGTTGATAATTCAGGGA+AGG + chr2.4:54122647-54122666 None:intergenic 35.0%
!! GAGGTGAATGTTGATAATTC+AGG + chr2.4:54122652-54122671 None:intergenic 35.0%
GAAACACCGAAAATCGCAAT+AGG + chr2.4:54123528-54123547 None:intergenic 40.0%
GATTAACACTGGCATTGTTC+AGG - chr2.4:54123470-54123489 MS.gene073414:CDS 40.0%
GGGGATAAACAAGGTAATTC+CGG + chr2.4:54123232-54123251 None:intergenic 40.0%
GGTGAAGATAGTGAGATTGT+AGG + chr2.4:54123037-54123056 None:intergenic 40.0%
GTGAGGTTGAAAGAGAAAGA+TGG + chr2.4:54122610-54122629 None:intergenic 40.0%
GTGATGTTGAGAAGAATGCT+TGG + chr2.4:54123114-54123133 None:intergenic 40.0%
GTTGTGAATGAATTCGAAGC+CGG - chr2.4:54123195-54123214 MS.gene073414:CDS 40.0%
TTAACACTGGCATTGTTCAG+GGG - chr2.4:54123472-54123491 MS.gene073414:CDS 40.0%
TTCGAATTCATTCACAACGC+CGG + chr2.4:54123193-54123212 None:intergenic 40.0%
! GAAAGAGAAAGATGGTGTTG+GGG + chr2.4:54122602-54122621 None:intergenic 40.0%
! GAGAACAACGGCTTTTTGAT+CGG + chr2.4:54123310-54123329 None:intergenic 40.0%
!! AACATGCGCTTTGAGAACAA+CGG + chr2.4:54123322-54123341 None:intergenic 40.0%
!! GAAATTGTGAGCATCGATGA+GGG + chr2.4:54123142-54123161 None:intergenic 40.0%
!! GGAATGGATTGTAATGGTGT+TGG + chr2.4:54122959-54122978 None:intergenic 40.0%
!! TGAAATTGTGAGCATCGATG+AGG + chr2.4:54123143-54123162 None:intergenic 40.0%
!! TTGTAATGGTGTTGGTGTTC+GGG + chr2.4:54122951-54122970 None:intergenic 40.0%
AATGAATTCGAAGCCGGTGA+AGG - chr2.4:54123201-54123220 MS.gene073414:CDS 45.0%
AGTGATTGAAGCGAAACGGA+AGG + chr2.4:54123282-54123301 None:intergenic 45.0%
ATTCAGGGAAGGTTGAAAGG+AGG + chr2.4:54122636-54122655 None:intergenic 45.0%
CCATGAGATCGAAACCGTAA+GGG + chr2.4:54123077-54123096 None:intergenic 45.0%
CTAACATCTTCACCAAACCC+TGG + chr2.4:54123699-54123718 None:intergenic 45.0%
CTTCGTGCTTATTACCCTCT+CGG - chr2.4:54123342-54123361 MS.gene073414:CDS 45.0%
GAAGAAGCAGCATCCTCATA+AGG + chr2.4:54123633-54123652 None:intergenic 45.0%
GGAGATTTCGCAGACACAAT+TGG + chr2.4:54123588-54123607 None:intergenic 45.0%
GGGAAGTGATTGAAGCGAAA+CGG + chr2.4:54123286-54123305 None:intergenic 45.0%
TTCAGGGAAGGTTGAAAGGA+GGG + chr2.4:54122635-54122654 None:intergenic 45.0%
TTTGAGCTGTGAAAGAACGG+TGG + chr2.4:54123007-54123026 None:intergenic 45.0%
TTTGAGGAAGAGTAAGGTGC+TGG + chr2.4:54122982-54123001 None:intergenic 45.0%
! AACAACGGCTTTTTGATCGG+CGG + chr2.4:54123307-54123326 None:intergenic 45.0%
! AGAAAGATGGTGTTGGGGAT+AGG + chr2.4:54122597-54122616 None:intergenic 45.0%
! ATGGTGTTGGGGATAGGATT+AGG + chr2.4:54122591-54122610 None:intergenic 45.0%
! TTGAGGAAGAGTAAGGTGCT+GGG + chr2.4:54122981-54123000 None:intergenic 45.0%
!! TGCTGGGGAATGGATTGTAA+TGG + chr2.4:54122965-54122984 None:intergenic 45.0%
!! TGTAATGGTGTTGGTGTTCG+GGG + chr2.4:54122950-54122969 None:intergenic 45.0%
!!! TGCGATTTTCGGTGTTTCGA+AGG - chr2.4:54123530-54123549 MS.gene073414:CDS 45.0%
AATTCCGGAACCACCTTCAC+CGG + chr2.4:54123217-54123236 None:intergenic 50.0%
ACATCTTCACCAAACCCTGG+CGG + chr2.4:54123696-54123715 None:intergenic 50.0%
ACCACTCTCTTCCAAACCAC+CGG - chr2.4:54122883-54122902 MS.gene073414:CDS 50.0%
ATCGTCGACGGGGATAAACA+AGG + chr2.4:54123241-54123260 None:intergenic 50.0%
CACATCTTTCTTCACCGCCA+GGG - chr2.4:54123679-54123698 MS.gene073414:CDS 50.0%
CCCTTACGGTTTCGATCTCA+TGG - chr2.4:54123074-54123093 MS.gene073414:CDS 50.0%
CGAAACGGAAGGAGGTAGAT+CGG + chr2.4:54123271-54123290 None:intergenic 50.0%
CGAGGAGAGAGAGTGAATTG+CGG + chr2.4:54122717-54122736 None:intergenic 50.0%
CGGTTTCGATCTCATGGCAT+CGG - chr2.4:54123080-54123099 MS.gene073414:CDS 50.0%
GAAGCAGCATCCTCATAAGG+AGG + chr2.4:54123630-54123649 None:intergenic 50.0%
GAGAGAGAGTGAATTGCGGT+GGG + chr2.4:54122713-54122732 None:intergenic 50.0%
GATTGAAGCGAAACGGAAGG+AGG + chr2.4:54123279-54123298 None:intergenic 50.0%
GCCATGAGATCGAAACCGTA+AGG + chr2.4:54123078-54123097 None:intergenic 50.0%
GGCGGTGAAGAAAGATGTGA+CGG + chr2.4:54123678-54123697 None:intergenic 50.0%
GGCTCTGTTGCGATTAACAC+TGG - chr2.4:54123459-54123478 MS.gene073414:CDS 50.0%
GTGGCGTTTGAGGAAGAGTA+AGG + chr2.4:54122988-54123007 None:intergenic 50.0%
GTTGACGGAGCCAAAGTTGT+TGG + chr2.4:54122917-54122936 None:intergenic 50.0%
TAGTGCTCCTCCTCCTTATG+AGG - chr2.4:54123617-54123636 MS.gene073414:CDS 50.0%
TCACATCTTTCTTCACCGCC+AGG - chr2.4:54123678-54123697 MS.gene073414:CDS 50.0%
TCGGTGGTGAGATAGGTGTT+GGG + chr2.4:54122745-54122764 None:intergenic 50.0%
TGAAAGAACGGTGGCGTTTG+AGG + chr2.4:54122998-54123017 None:intergenic 50.0%
TGGATTGAAGCGAACCGAGA+GGG + chr2.4:54123359-54123378 None:intergenic 50.0%
TGTGTCTGCGAAATCTCCAC+CGG - chr2.4:54123590-54123609 MS.gene073414:CDS 50.0%
TTCATTCACAACGCCGGAAG+CGG + chr2.4:54123187-54123206 None:intergenic 50.0%
! ACAACGGCTTTTTGATCGGC+GGG + chr2.4:54123306-54123325 None:intergenic 50.0%
! AGGGGGTGTGGAAGAAAGTA+GGG + chr2.4:54122674-54122693 None:intergenic 50.0%
! GAGAGTGGTGACGAGTTTAC+CGG + chr2.4:54122872-54122891 None:intergenic 50.0%
!! AAGAGTAAGGTGCTGGGGAA+TGG + chr2.4:54122975-54122994 None:intergenic 50.0%
!! TGAGGAAGAGTAAGGTGCTG+GGG + chr2.4:54122980-54122999 None:intergenic 50.0%
!!! GTGAGCATCGATGAGGGTTT+TGG + chr2.4:54123136-54123155 None:intergenic 50.0%
ACGCTTGCGACGGAAGCTAT+GGG - chr2.4:54123399-54123418 MS.gene073414:CDS 55.0%
ACTGGAGGAGGATGTGGTAG+CGG + chr2.4:54122813-54122832 None:intergenic 55.0%
AGAGGAACTGGAGGAGGATG+TGG + chr2.4:54122819-54122838 None:intergenic 55.0%
AGGGAAGGTTGAAAGGAGGG+AGG + chr2.4:54122632-54122651 None:intergenic 55.0%
AGGTTGAAAGGAGGGAGGTG+AGG + chr2.4:54122627-54122646 None:intergenic 55.0%
ATAGCTTCCGTCGCAAGCGT+CGG + chr2.4:54123399-54123418 None:intergenic 55.0%
ATTGCGGTGGGAGAGATCAG+CGG + chr2.4:54122701-54122720 None:intergenic 55.0%
CTTTCTTCACCGCCAGGGTT+TGG - chr2.4:54123684-54123703 MS.gene073414:CDS 55.0%
GAATTCGAAGCCGGTGAAGG+TGG - chr2.4:54123204-54123223 MS.gene073414:CDS 55.0%
GACGGAGCCAAAGTTGTTGG+TGG + chr2.4:54122914-54122933 None:intergenic 55.0%
GAGGTTATGGAGGTGAGGGT+CGG + chr2.4:54122764-54122783 None:intergenic 55.0%
GCAGCATCCTCATAAGGAGG+AGG + chr2.4:54123627-54123646 None:intergenic 55.0%
GCTTCAATGCTCTCCGCTTC+CGG - chr2.4:54123171-54123190 MS.gene073414:CDS 55.0%
GGAGAGAGAGTGAATTGCGG+TGG + chr2.4:54122714-54122733 None:intergenic 55.0%
GGCTGTACATCAAAACCCGC+CGG + chr2.4:54123657-54123676 None:intergenic 55.0%
GGGAGAGGTTATGGAGGTGA+GGG + chr2.4:54122768-54122787 None:intergenic 55.0%
GGGTGTGGAAGAAAGTAGGG+AGG + chr2.4:54122671-54122690 None:intergenic 55.0%
GTCGGTGGTGAGATAGGTGT+TGG + chr2.4:54122746-54122765 None:intergenic 55.0%
TGACGAGTTTACCGGAGGGA+GGG + chr2.4:54122864-54122883 None:intergenic 55.0%
! AGTGGTGACGAGTTTACCGG+AGG + chr2.4:54122869-54122888 None:intergenic 55.0%
! CGGTGGTGAGATAGGTGTTG+GGG + chr2.4:54122744-54122763 None:intergenic 55.0%
! GAGGGGGTGTGGAAGAAAGT+AGG + chr2.4:54122675-54122694 None:intergenic 55.0%
! GTGGATTGAAGCGAACCGAG+AGG + chr2.4:54123360-54123379 None:intergenic 55.0%
! GTGGTGACGAGTTTACCGGA+GGG + chr2.4:54122868-54122887 None:intergenic 55.0%
! TGAGGATGCTGCTTCTTCGC+CGG - chr2.4:54123635-54123654 MS.gene073414:CDS 55.0%
!! AGCCAAAGTTGTTGGTGGCC+CGG + chr2.4:54122909-54122928 None:intergenic 55.0%
!! ATCGGCGAAAGCATCGTCGA+CGG + chr2.4:54123253-54123272 None:intergenic 55.0%
!! GGTGAGATAGGTGTTGGGGA+CGG + chr2.4:54122740-54122759 None:intergenic 55.0%
ACCAGGAGAGGAACTGGAGG+AGG + chr2.4:54122825-54122844 None:intergenic 60.0%
AGGATGTCAGCGAGAGCGGA+GGG + chr2.4:54122790-54122809 None:intergenic 60.0%
CGGGGAGTGGGTGATGTTGA+CGG + chr2.4:54122932-54122951 None:intergenic 60.0%
CTCTCTTCCAAACCACCGGC+CGG - chr2.4:54122887-54122906 MS.gene073414:CDS 60.0%
GAAGCCGGTGAAGGTGGTTC+CGG - chr2.4:54123210-54123229 MS.gene073414:CDS 60.0%
GACGCTTGCGACGGAAGCTA+TGG - chr2.4:54123398-54123417 MS.gene073414:CDS 60.0%
GAGAGATCAGCGGTGAGAGG+GGG + chr2.4:54122691-54122710 None:intergenic 60.0%
GGAGAGATCAGCGGTGAGAG+GGG + chr2.4:54122692-54122711 None:intergenic 60.0%
GGGAGAGATCAGCGGTGAGA+GGG + chr2.4:54122693-54122712 None:intergenic 60.0%
GGGGAGAGGTTATGGAGGTG+AGG + chr2.4:54122769-54122788 None:intergenic 60.0%
GTGACGAGTTTACCGGAGGG+AGG + chr2.4:54122865-54122884 None:intergenic 60.0%
GTGAGGGTCGGTGGTGAGAT+AGG + chr2.4:54122752-54122771 None:intergenic 60.0%
GTTATGGAGGTGAGGGTCGG+TGG + chr2.4:54122761-54122780 None:intergenic 60.0%
GTTGCAACCGACGCTTGCGA+CGG - chr2.4:54123389-54123408 MS.gene073414:CDS 60.0%
TCCTCCTCCAGTTCCTCTCC+TGG - chr2.4:54122821-54122840 MS.gene073414:CDS 60.0%
TCTCTTCCAAACCACCGGCC+GGG - chr2.4:54122888-54122907 MS.gene073414:CDS 60.0%
TGGGAGAGATCAGCGGTGAG+AGG + chr2.4:54122694-54122713 None:intergenic 60.0%
! CGGTTGCAACGGATTCGCAG+TGG + chr2.4:54123379-54123398 None:intergenic 60.0%
! GCCGGTGGTTTGGAAGAGAG+TGG + chr2.4:54122887-54122906 None:intergenic 60.0%
!! AAAGTTGTTGGTGGCCCGGC+CGG + chr2.4:54122905-54122924 None:intergenic 60.0%
!! GGAGGAGGAGCACTACTGTC+CGG + chr2.4:54123612-54123631 None:intergenic 60.0%
!! GGTGTTGGTGTTCGGGGAGT+GGG + chr2.4:54122944-54122963 None:intergenic 60.0%
!! GTCGCAAGCGTCGGTTGCAA+CGG + chr2.4:54123390-54123409 None:intergenic 60.0%
!! TCGGCGAAAGCATCGTCGAC+GGG + chr2.4:54123252-54123271 None:intergenic 60.0%
!! TGGTGTTGGTGTTCGGGGAG+TGG + chr2.4:54122945-54122964 None:intergenic 60.0%
ATCAGCGGTGAGAGGGGGTG+TGG + chr2.4:54122686-54122705 None:intergenic 65.0%
CGAGTTTACCGGAGGGAGGG+AGG + chr2.4:54122861-54122880 None:intergenic 65.0%
CGGACCAGGAGAGGAACTGG+AGG + chr2.4:54122828-54122847 None:intergenic 65.0%
CGGAGGGGGAGAGGTTATGG+AGG + chr2.4:54122774-54122793 None:intergenic 65.0%
CTCCAACACCTCCCTCCCTC+CGG - chr2.4:54122850-54122869 MS.gene073414:CDS 65.0%
GAGCGGAGGGGGAGAGGTTA+TGG + chr2.4:54122777-54122796 None:intergenic 65.0%
GAGGATGTCAGCGAGAGCGG+AGG + chr2.4:54122791-54122810 None:intergenic 65.0%
GATGTCAGCGAGAGCGGAGG+GGG + chr2.4:54122788-54122807 None:intergenic 65.0%
GCGACGGAAGCTATGGGTGC+TGG - chr2.4:54123405-54123424 MS.gene073414:CDS 65.0%
GCGGAGGATGTCAGCGAGAG+CGG + chr2.4:54122794-54122813 None:intergenic 65.0%
GGATGTCAGCGAGAGCGGAG+GGG + chr2.4:54122789-54122808 None:intergenic 65.0%
GGCCGGGCCACCAACAACTT+TGG - chr2.4:54122904-54122923 MS.gene073414:CDS 65.0%
GGTCGGACCAGGAGAGGAAC+TGG + chr2.4:54122831-54122850 None:intergenic 65.0%
GTTGGAGGTCGGACCAGGAG+AGG + chr2.4:54122837-54122856 None:intergenic 65.0%
! GATGCTGCTTCTTCGCCGGC+GGG - chr2.4:54123639-54123658 MS.gene073414:CDS 65.0%
! GGATGCTGCTTCTTCGCCGG+CGG - chr2.4:54123638-54123657 MS.gene073414:CDS 65.0%
! TACCGGAGGGAGGGAGGTGT+TGG + chr2.4:54122855-54122874 None:intergenic 65.0%
!! CGGCGAAAGCATCGTCGACG+GGG + chr2.4:54123251-54123270 None:intergenic 65.0%
!! GAGGTGTTGGAGGTCGGACC+AGG + chr2.4:54122842-54122861 None:intergenic 65.0%
!! GATAGGTGTTGGGGACGGCG+AGG + chr2.4:54122735-54122754 None:intergenic 65.0%
!! GGAGGAGCACTACTGTCCGG+TGG + chr2.4:54123609-54123628 None:intergenic 65.0%
!! GGAGGAGGATGTGGTAGCGG+AGG + chr2.4:54122810-54122829 None:intergenic 65.0%
!! GGGAGGGAGGTGTTGGAGGT+CGG + chr2.4:54122848-54122867 None:intergenic 65.0%
!! CGGAGGGAGGGAGGTGTTGG+AGG + chr2.4:54122852-54122871 None:intergenic 70.0%
CAGCGAGAGCGGAGGGGGAG+AGG + chr2.4:54122783-54122802 None:intergenic 75.0%
TGGTGGCCCGGCCGGTGGTT+TGG + chr2.4:54122897-54122916 None:intergenic 75.0%
!! GTTGTTGGTGGCCCGGCCGG+TGG + chr2.4:54122902-54122921 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 54122577 54123804 54122577 ID=MS.gene073414
chr2.4 mRNA 54122577 54123804 54122577 ID=MS.gene073414.t1;Parent=MS.gene073414
chr2.4 exon 54122668 54123804 54122668 ID=MS.gene073414.t1.exon1;Parent=MS.gene073414.t1
chr2.4 CDS 54122668 54123804 54122668 ID=cds.MS.gene073414.t1;Parent=MS.gene073414.t1
chr2.4 exon 54122577 54122588 54122577 ID=MS.gene073414.t1.exon2;Parent=MS.gene073414.t1
chr2.4 CDS 54122577 54122588 54122577 ID=cds.MS.gene073414.t1;Parent=MS.gene073414.t1
Gene Sequence

>MS.gene073414

ATGCTATATTTCCTAATCCTATCCCCAACACCATCTTTCTCTTTCAACCTCACCTCCCTCCTTTCAACCTTCCCTGAATTATCAACATTCACCTCCCTACTTTCTTCCACACCCCCTCTCACCGCTGATCTCTCCCACCGCAATTCACTCTCTCTCCTCGCCGTCCCCAACACCTATCTCACCACCGACCCTCACCTCCATAACCTCTCCCCCTCCGCTCTCGCTGACATCCTCCGCTACCACATCCTCCTCCAGTTCCTCTCCTGGTCCGACCTCCAACACCTCCCTCCCTCCGGTAAACTCGTCACCACTCTCTTCCAAACCACCGGCCGGGCCACCAACAACTTTGGCTCCGTCAACATCACCCACTCCCCGAACACCAACACCATTACAATCCATTCCCCAGCACCTTACTCTTCCTCAAACGCCACCGTTCTTTCACAGCTCAAAATGTTACCCTACAATCTCACTATCTTCACCGTTGATTCTCTTTTAATCCCTTACGGTTTCGATCTCATGGCATCGGAAACTCGTCCAAGCATTCTTCTCAACATCACCAAAACCCTCATCGATGCTCACAATTTCAACGTCGCTGCTTCAATGCTCTCCGCTTCCGGCGTTGTGAATGAATTCGAAGCCGGTGAAGGTGGTTCCGGAATTACCTTGTTTATCCCCGTCGACGATGCTTTCGCCGATCTACCTCCTTCCGTTTCGCTTCAATCACTTCCCGCCGATCAAAAAGCCGTTGTTCTCAAAGCGCATGTTCTTCGTGCTTATTACCCTCTCGGTTCGCTTCAATCCACTGCGAATCCGTTGCAACCGACGCTTGCGACGGAAGCTATGGGTGCTGGAAGTTTCACGTTGAATATTTCAACTTTTAATGGCTCTGTTGCGATTAACACTGGCATTGTTCAGGGGATAATTACTCAGACAGTGTTTGATCAGAATCCTATTGCGATTTTCGGTGTTTCGAAGGTTTTGTTGCCAAGAGAGATATTTGGGAAGAATCCAATTGTGTCTGCGAAATCTCCACCGGACAGTAGTGCTCCTCCTCCTTATGAGGATGCTGCTTCTTCGCCGGCGGGTTTTGATGTACAGCCGTCACATCTTTCTTCACCGCCAGGGTTTGGTGAAGATGTTAGCTCAGATGTTGCAAGTGTTTATGGTTCTGAGTTATTTGTTTTTCTACTTTGTTGTATAAATTTGTATTTAATAGTATAAAGAGTAA
Protein sequence

>MS.gene073414.t1

MLYFLILSPTPSFSFNLTSLLSTFPELSTFTSLLSSTPPLTADLSHRNSLSLLAVPNTYLTTDPHLHNLSPSALADILRYHILLQFLSWSDLQHLPPSGKLVTTLFQTTGRATNNFGSVNITHSPNTNTITIHSPAPYSSSNATVLSQLKMLPYNLTIFTVDSLLIPYGFDLMASETRPSILLNITKTLIDAHNFNVAASMLSASGVVNEFEAGEGGSGITLFIPVDDAFADLPPSVSLQSLPADQKAVVLKAHVLRAYYPLGSLQSTANPLQPTLATEAMGAGSFTLNISTFNGSVAINTGIVQGIITQTVFDQNPIAIFGVSKVLLPREIFGKNPIVSAKSPPDSSAPPPYEDAASSPAGFDVQPSHLSSPPGFGEDYKE