Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073927.t1 | XP_003590175.2 | 94.5 | 145 | 8 | 0 | 1 | 145 | 152 | 296 | 1.70E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073927.t1 | G7I6P2 | 94.5 | 145 | 8 | 0 | 1 | 145 | 152 | 296 | 1.2e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050507 | MS.gene073927 | 0.856457 | 3.18E-62 | -1.69E-46 |
| MS.gene051617 | MS.gene073927 | 0.836347 | 9.87E-57 | -1.69E-46 |
| MS.gene056903 | MS.gene073927 | 0.84526 | 4.54E-59 | -1.69E-46 |
| MS.gene058748 | MS.gene073927 | 0.84682 | 1.71E-59 | -1.69E-46 |
| MS.gene05934 | MS.gene073927 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene061041 | MS.gene073927 | 0.807538 | 4.81E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073927.t1 | MTR_1g045520 | 95.862 | 145 | 6 | 0 | 1 | 145 | 152 | 296 | 1.08e-85 | 253 |
| MS.gene073927.t1 | MTR_4g088590 | 65.060 | 83 | 5 | 1 | 1 | 83 | 74 | 132 | 1.48e-16 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073927.t1 | AT3G04310 | 35.948 | 153 | 81 | 4 | 1 | 145 | 125 | 268 | 4.89e-20 | 83.6 |
| MS.gene073927.t1 | AT3G04310 | 35.948 | 153 | 81 | 4 | 1 | 145 | 125 | 268 | 4.89e-20 | 83.6 |
Find 42 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTGGCAATTGAGAAATTA+TGG | 0.159956 | 1.4:+29746182 | MS.gene073927:CDS |
| AGCCGAGATGCACCAAGTTT+TGG | 0.334550 | 1.4:+29746373 | MS.gene073927:CDS |
| GCTTGTGATCTCCTTGTTGT+TGG | 0.339884 | 1.4:-29746424 | None:intergenic |
| TTCATCCTATACTTGCGCTT+TGG | 0.407380 | 1.4:-29746223 | None:intergenic |
| CCCGTGTGTCATTTACATTT+CGG | 0.416520 | 1.4:-29746255 | None:intergenic |
| AGGGTCACAAAGGATGAATA+TGG | 0.435338 | 1.4:+29746453 | MS.gene073927:CDS |
| CTTGCGCTTTGGTTTGCTCT+CGG | 0.443826 | 1.4:-29746212 | None:intergenic |
| ACTGGCAATTGAGAAATTAT+GGG | 0.445083 | 1.4:+29746183 | MS.gene073927:CDS |
| AAAACTAATACGAGGAAGGC+GGG | 0.468188 | 1.4:+29746304 | MS.gene073927:CDS |
| GCAAACCAAAGCGCAAGTAT+AGG | 0.470582 | 1.4:+29746218 | MS.gene073927:CDS |
| TGGAAAGTGGTAGGTTGAGT+AGG | 0.473865 | 1.4:+29746473 | MS.gene073927:CDS |
| TGTAAATGACACACGGGTTG+AGG | 0.476945 | 1.4:+29746261 | MS.gene073927:CDS |
| GGAAGGCGGGGATGCAGTCG+TGG | 0.477673 | 1.4:+29746317 | MS.gene073927:CDS |
| TCATCCCATCCACCAAAACT+TGG | 0.498543 | 1.4:-29746385 | None:intergenic |
| CACCAAAACTTGGTGCATCT+CGG | 0.507741 | 1.4:-29746375 | None:intergenic |
| CAGTCAGTCAGCACTTGCAC+TGG | 0.515720 | 1.4:+29746165 | MS.gene073927:CDS |
| AACAACGGTTCGGTTGATAG+TGG | 0.539590 | 1.4:+29746349 | MS.gene073927:CDS |
| GGAGATCACAAGCAAGGAAA+GGG | 0.542632 | 1.4:+29746434 | MS.gene073927:CDS |
| AGGAGATCACAAGCAAGGAA+AGG | 0.559152 | 1.4:+29746433 | MS.gene073927:CDS |
| AATAAGCAATCGAACCAAGA+GGG | 0.559725 | 1.4:-29746518 | None:intergenic |
| CAAAGGATGAATATGGAAAG+TGG | 0.560093 | 1.4:+29746460 | MS.gene073927:CDS |
| GGTAGTTGAGAACAACGGTT+CGG | 0.582840 | 1.4:+29746339 | MS.gene073927:CDS |
| AGCGCAAGTATAGGATGAAG+AGG | 0.587300 | 1.4:+29746227 | MS.gene073927:CDS |
| GGGATGACTTGGAGAGAGCA+AGG | 0.588570 | 1.4:+29746401 | MS.gene073927:CDS |
| GAAGGCGGGGATGCAGTCGT+GGG | 0.589601 | 1.4:+29746318 | MS.gene073927:CDS |
| GGATGAATATGGAAAGTGGT+AGG | 0.598330 | 1.4:+29746464 | MS.gene073927:CDS |
| CAACAAGGAGATCACAAGCA+AGG | 0.612370 | 1.4:+29746428 | MS.gene073927:CDS |
| CAATTGAGAAATTATGGGGA+CGG | 0.615205 | 1.4:+29746188 | MS.gene073927:CDS |
| AGAGAGCAAGGCCAACAACA+AGG | 0.623233 | 1.4:+29746413 | MS.gene073927:CDS |
| CCAGAAAACTAATACGAGGA+AGG | 0.626614 | 1.4:+29746300 | MS.gene073927:CDS |
| GAAAACTAATACGAGGAAGG+CGG | 0.634403 | 1.4:+29746303 | MS.gene073927:CDS |
| AAACTAATACGAGGAAGGCG+GGG | 0.635407 | 1.4:+29746305 | MS.gene073927:CDS |
| GCCGAAATGTAAATGACACA+CGG | 0.641802 | 1.4:+29746254 | MS.gene073927:CDS |
| AAGCAAGGAAAGGGTCACAA+AGG | 0.643486 | 1.4:+29746443 | MS.gene073927:CDS |
| ATAAGCAATCGAACCAAGAG+GGG | 0.649272 | 1.4:-29746517 | None:intergenic |
| CCGAAATGTAAATGACACAC+GGG | 0.650247 | 1.4:+29746255 | MS.gene073927:CDS |
| CTGGCAATTGAGAAATTATG+GGG | 0.651354 | 1.4:+29746184 | MS.gene073927:CDS |
| GCAAGTGCTGACTGACTGAG+AGG | 0.659866 | 1.4:-29746160 | None:intergenic |
| CAAGTGCTGACTGACTGAGA+GGG | 0.679661 | 1.4:-29746159 | None:intergenic |
| CAATAAGCAATCGAACCAAG+AGG | 0.681035 | 1.4:-29746519 | None:intergenic |
| AAACCCAGAAAACTAATACG+AGG | 0.734217 | 1.4:+29746296 | MS.gene073927:CDS |
| TCGTGGGTAGTTGAGAACAA+CGG | 0.734928 | 1.4:+29746334 | MS.gene073927:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGCTATTTTTCCATTTTT+GGG | + | chr1.4:29746538-29746557 | MS.gene073927:CDS | 20.0% |
| !!! | TATTGCTATTTTTCCATTTT+TGG | + | chr1.4:29746537-29746556 | MS.gene073927:CDS | 20.0% |
| ACTGGCAATTGAGAAATTAT+GGG | + | chr1.4:29746183-29746202 | MS.gene073927:CDS | 30.0% | |
| AAACCCAGAAAACTAATACG+AGG | + | chr1.4:29746296-29746315 | MS.gene073927:CDS | 35.0% | |
| AATAAGCAATCGAACCAAGA+GGG | - | chr1.4:29746521-29746540 | None:intergenic | 35.0% | |
| CAAAGGATGAATATGGAAAG+TGG | + | chr1.4:29746460-29746479 | MS.gene073927:CDS | 35.0% | |
| CAATTGAGAAATTATGGGGA+CGG | + | chr1.4:29746188-29746207 | MS.gene073927:CDS | 35.0% | |
| CACTGGCAATTGAGAAATTA+TGG | + | chr1.4:29746182-29746201 | MS.gene073927:CDS | 35.0% | |
| CTGGCAATTGAGAAATTATG+GGG | + | chr1.4:29746184-29746203 | MS.gene073927:CDS | 35.0% | |
| !! | TTAGTTTTCTGGGTTTCTTC+TGG | - | chr1.4:29746292-29746311 | None:intergenic | 35.0% |
| !! | TTTTTCCATTTTTGGGCACA+TGG | + | chr1.4:29746545-29746564 | MS.gene073927:CDS | 35.0% |
| !!! | CTTCCTCGTATTAGTTTTCT+GGG | - | chr1.4:29746302-29746321 | None:intergenic | 35.0% |
| AAAACTAATACGAGGAAGGC+GGG | + | chr1.4:29746304-29746323 | MS.gene073927:CDS | 40.0% | |
| AGGGTCACAAAGGATGAATA+TGG | + | chr1.4:29746453-29746472 | MS.gene073927:CDS | 40.0% | |
| ATAAGCAATCGAACCAAGAG+GGG | - | chr1.4:29746520-29746539 | None:intergenic | 40.0% | |
| CAATAAGCAATCGAACCAAG+AGG | - | chr1.4:29746522-29746541 | None:intergenic | 40.0% | |
| CCAGAAAACTAATACGAGGA+AGG | + | chr1.4:29746300-29746319 | MS.gene073927:CDS | 40.0% | |
| CCGAAATGTAAATGACACAC+GGG | + | chr1.4:29746255-29746274 | MS.gene073927:CDS | 40.0% | |
| GAAAACTAATACGAGGAAGG+CGG | + | chr1.4:29746303-29746322 | MS.gene073927:CDS | 40.0% | |
| GCCGAAATGTAAATGACACA+CGG | + | chr1.4:29746254-29746273 | MS.gene073927:CDS | 40.0% | |
| GGATGAATATGGAAAGTGGT+AGG | + | chr1.4:29746464-29746483 | MS.gene073927:CDS | 40.0% | |
| TTCATCCTATACTTGCGCTT+TGG | - | chr1.4:29746226-29746245 | None:intergenic | 40.0% | |
| TTTGTCCATGTGCCCAAAAA+TGG | - | chr1.4:29746553-29746572 | None:intergenic | 40.0% | |
| !! | CCCGTGTGTCATTTACATTT+CGG | - | chr1.4:29746258-29746277 | None:intergenic | 40.0% |
| !!! | CCTTCCTCGTATTAGTTTTC+TGG | - | chr1.4:29746303-29746322 | None:intergenic | 40.0% |
| AAAAAGTGATACGCCCCTCT+TGG | + | chr1.4:29746504-29746523 | MS.gene073927:CDS | 45.0% | |
| AAACTAATACGAGGAAGGCG+GGG | + | chr1.4:29746305-29746324 | MS.gene073927:CDS | 45.0% | |
| AAGCAAGGAAAGGGTCACAA+AGG | + | chr1.4:29746443-29746462 | MS.gene073927:CDS | 45.0% | |
| AGCGCAAGTATAGGATGAAG+AGG | + | chr1.4:29746227-29746246 | MS.gene073927:CDS | 45.0% | |
| AGGAGATCACAAGCAAGGAA+AGG | + | chr1.4:29746433-29746452 | MS.gene073927:CDS | 45.0% | |
| CAACAAGGAGATCACAAGCA+AGG | + | chr1.4:29746428-29746447 | MS.gene073927:CDS | 45.0% | |
| GCAAACCAAAGCGCAAGTAT+AGG | + | chr1.4:29746218-29746237 | MS.gene073927:CDS | 45.0% | |
| GGAGATCACAAGCAAGGAAA+GGG | + | chr1.4:29746434-29746453 | MS.gene073927:CDS | 45.0% | |
| GGTAGTTGAGAACAACGGTT+CGG | + | chr1.4:29746339-29746358 | MS.gene073927:CDS | 45.0% | |
| TCATCCCATCCACCAAAACT+TGG | - | chr1.4:29746388-29746407 | None:intergenic | 45.0% | |
| TCGTGGGTAGTTGAGAACAA+CGG | + | chr1.4:29746334-29746353 | MS.gene073927:CDS | 45.0% | |
| ! | GCTTGTGATCTCCTTGTTGT+TGG | - | chr1.4:29746427-29746446 | None:intergenic | 45.0% |
| ! | TGGAAAGTGGTAGGTTGAGT+AGG | + | chr1.4:29746473-29746492 | MS.gene073927:CDS | 45.0% |
| ! | TGTAAATGACACACGGGTTG+AGG | + | chr1.4:29746261-29746280 | MS.gene073927:CDS | 45.0% |
| !! | AACAACGGTTCGGTTGATAG+TGG | + | chr1.4:29746349-29746368 | MS.gene073927:CDS | 45.0% |
| !! | ATGCACCAAGTTTTGGTGGA+TGG | + | chr1.4:29746380-29746399 | MS.gene073927:CDS | 45.0% |
| !! | CACCAAAACTTGGTGCATCT+CGG | - | chr1.4:29746378-29746397 | None:intergenic | 45.0% |
| !! | TGCACCAAGTTTTGGTGGAT+GGG | + | chr1.4:29746381-29746400 | MS.gene073927:CDS | 45.0% |
| !! | TTTTGGTGGATGGGATGACT+TGG | + | chr1.4:29746390-29746409 | MS.gene073927:CDS | 45.0% |
| AGAGAGCAAGGCCAACAACA+AGG | + | chr1.4:29746413-29746432 | MS.gene073927:CDS | 50.0% | |
| ! | AGCCGAGATGCACCAAGTTT+TGG | + | chr1.4:29746373-29746392 | MS.gene073927:CDS | 50.0% |
| !! | CAAGTGCTGACTGACTGAGA+GGG | - | chr1.4:29746162-29746181 | None:intergenic | 50.0% |
| !! | CGAGATGCACCAAGTTTTGG+TGG | + | chr1.4:29746376-29746395 | MS.gene073927:CDS | 50.0% |
| !! | CTTGCGCTTTGGTTTGCTCT+CGG | - | chr1.4:29746215-29746234 | None:intergenic | 50.0% |
| CAGTCAGTCAGCACTTGCAC+TGG | + | chr1.4:29746165-29746184 | MS.gene073927:CDS | 55.0% | |
| ! | GGGATGACTTGGAGAGAGCA+AGG | + | chr1.4:29746401-29746420 | MS.gene073927:CDS | 55.0% |
| !! | GCAAGTGCTGACTGACTGAG+AGG | - | chr1.4:29746163-29746182 | None:intergenic | 55.0% |
| GAAGGCGGGGATGCAGTCGT+GGG | + | chr1.4:29746318-29746337 | MS.gene073927:CDS | 65.0% | |
| GGAAGGCGGGGATGCAGTCG+TGG | + | chr1.4:29746317-29746336 | MS.gene073927:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 29746145 | 29746582 | 29746145 | ID=MS.gene073927 |
| chr1.4 | mRNA | 29746145 | 29746582 | 29746145 | ID=MS.gene073927.t1;Parent=MS.gene073927 |
| chr1.4 | exon | 29746145 | 29746582 | 29746145 | ID=MS.gene073927.t1.exon1;Parent=MS.gene073927.t1 |
| chr1.4 | CDS | 29746145 | 29746582 | 29746145 | ID=cds.MS.gene073927.t1;Parent=MS.gene073927.t1 |
| Gene Sequence |
| Protein sequence |
