Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073948.t1 | XP_003590190.1 | 99.1 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 2.00E-265 | 924.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073948.t1 | Q94AR8 | 78.8 | 463 | 91 | 3 | 3 | 459 | 2 | 463 | 3.3e-216 | 752.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073948.t1 | G7I6Q7 | 99.1 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 1.4e-265 | 924.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene073948 | MS.gene48297 | PPI |
| MS.gene033323 | MS.gene073948 | PPI |
| MS.gene059965 | MS.gene073948 | PPI |
| MS.gene073948 | MS.gene011362 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073948.t1 | MTR_1g045750 | 99.129 | 459 | 4 | 0 | 1 | 459 | 1 | 459 | 0.0 | 953 |
| MS.gene073948.t1 | MTR_1g073200 | 68.476 | 479 | 122 | 6 | 8 | 459 | 5 | 481 | 0.0 | 673 |
| MS.gene073948.t1 | MTR_1g045760 | 48.000 | 125 | 46 | 1 | 107 | 231 | 52 | 157 | 7.12e-27 | 106 |
| MS.gene073948.t1 | MTR_4g048190 | 27.488 | 211 | 134 | 5 | 166 | 360 | 264 | 471 | 2.88e-15 | 79.0 |
| MS.gene073948.t1 | MTR_2g088360 | 27.907 | 215 | 128 | 6 | 166 | 360 | 264 | 471 | 7.02e-15 | 77.8 |
| MS.gene073948.t1 | MTR_5g022940 | 27.907 | 215 | 128 | 6 | 166 | 360 | 184 | 391 | 1.54e-13 | 73.6 |
| MS.gene073948.t1 | MTR_5g022940 | 27.907 | 215 | 128 | 6 | 166 | 360 | 80 | 287 | 1.61e-13 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073948.t1 | AT4G13430 | 78.834 | 463 | 91 | 3 | 3 | 459 | 2 | 463 | 0.0 | 778 |
| MS.gene073948.t1 | AT4G26970 | 25.000 | 288 | 182 | 8 | 108 | 365 | 206 | 489 | 1.70e-15 | 79.7 |
| MS.gene073948.t1 | AT2G05710 | 27.488 | 211 | 134 | 6 | 166 | 360 | 275 | 482 | 1.03e-14 | 77.4 |
| MS.gene073948.t1 | AT4G35830 | 23.134 | 268 | 176 | 6 | 122 | 360 | 21 | 287 | 1.05e-12 | 70.9 |
| MS.gene073948.t1 | AT4G35830 | 23.134 | 268 | 176 | 6 | 122 | 360 | 124 | 390 | 1.21e-12 | 70.9 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACACCTGGGGATAATATT+TGG | 0.139762 | 1.1:+28461098 | MS.gene073948:CDS |
| TCATTTACCTTACAGATTAT+TGG | 0.189248 | 1.1:+28463857 | MS.gene073948:intron |
| TAGATTTGATGTCTCAAAAT+TGG | 0.219082 | 1.1:+28468012 | MS.gene073948:CDS |
| TGCGGGCATAAGTATCTTTC+GGG | 0.251693 | 1.1:-28468924 | None:intergenic |
| GTTTGTGGCCCTGGTTCTAT+TGG | 0.265255 | 1.1:+28461151 | MS.gene073948:CDS |
| TTGCAGACCTTCTGTGTTGC+AGG | 0.266524 | 1.1:-28468668 | None:intergenic |
| TCCTAGTTGTGGTGCTTGTT+TGG | 0.267456 | 1.1:+28468897 | MS.gene073948:CDS |
| GTTCTTTCTGCAGGTTCTCT+TGG | 0.284191 | 1.1:+28462551 | MS.gene073948:intron |
| TCTATATTTGCAGGGTAAAA+AGG | 0.306488 | 1.1:+28468622 | MS.gene073948:intron |
| TCTTTGTCTACCTTGCTTGC+TGG | 0.328037 | 1.1:-28467448 | None:intergenic |
| GCTTGCTGGTCACTATATAC+TGG | 0.339417 | 1.1:-28467434 | None:intergenic |
| TTCTTTCTGCAGGTTCTCTT+GGG | 0.341117 | 1.1:+28462552 | MS.gene073948:intron |
| ATGCGGGCATAAGTATCTTT+CGG | 0.341407 | 1.1:-28468925 | None:intergenic |
| TGGCAAACACCTTTGTAATC+TGG | 0.349547 | 1.1:-28462409 | None:intergenic |
| ACTTGTACTGCTGGAGCATT+TGG | 0.355022 | 1.1:+28462588 | MS.gene073948:CDS |
| AGTCTTCCTGTCCCTGGATC+TGG | 0.355783 | 1.1:+28468820 | MS.gene073948:CDS |
| TATTTATATGTAATGCTATC+TGG | 0.356860 | 1.1:-28464231 | None:intergenic |
| TTTGTGGCCCTGGTTCTATT+GGG | 0.356911 | 1.1:+28461152 | MS.gene073948:CDS |
| CCTAGTTGTGGTGCTTGTTT+GGG | 0.357402 | 1.1:+28468898 | MS.gene073948:CDS |
| TACACCTGGGGATAATATTT+GGG | 0.359970 | 1.1:+28461099 | MS.gene073948:CDS |
| GCATTTGGTCAATTTGCAAC+AGG | 0.376621 | 1.1:+28462603 | MS.gene073948:CDS |
| ACTCATGATGTTTGTGGCCC+TGG | 0.393336 | 1.1:+28461142 | MS.gene073948:CDS |
| TTGCTCAGGAAGGTCATTGC+AGG | 0.404340 | 1.1:+28462440 | MS.gene073948:CDS |
| TACAAATCAATGGAGTTTGT+TGG | 0.414163 | 1.1:+28463905 | MS.gene073948:CDS |
| CAGCGTCAACCTTCTTCAAC+TGG | 0.418217 | 1.1:+28460912 | MS.gene073948:CDS |
| GACTATGACTGAGAAGATAA+TGG | 0.419716 | 1.1:+28461051 | MS.gene073948:CDS |
| CCCCGCACCCAACACAAAAC+CGG | 0.419745 | 1.1:-28462643 | None:intergenic |
| TGCAACATGGTTGTTGAAGC+TGG | 0.422479 | 1.1:+28464186 | MS.gene073948:CDS |
| TCCAACTTTGAGATTTGTAA+TGG | 0.425112 | 1.1:+28463713 | MS.gene073948:CDS |
| CTTGCCAAAGCACGGTTATC+CGG | 0.433090 | 1.1:-28468158 | None:intergenic |
| TCCATTACAAATCTCAAAGT+TGG | 0.438795 | 1.1:-28463714 | None:intergenic |
| GGTCAATTTGCAACAGGGAT+TGG | 0.439036 | 1.1:+28462609 | MS.gene073948:CDS |
| TAAACTAATCGAACCTCTCC+TGG | 0.452154 | 1.1:-28462463 | None:intergenic |
| TTGCCACATCGCTCTTGCTC+AGG | 0.452572 | 1.1:+28462426 | MS.gene073948:CDS |
| TCACTATATACTGGTTCATA+TGG | 0.463569 | 1.1:-28467425 | None:intergenic |
| CAGGAAGGTCATTGCAGGCC+AGG | 0.469342 | 1.1:+28462445 | MS.gene073948:CDS |
| CAAGTCTTACCACCAGATCC+AGG | 0.471556 | 1.1:-28468832 | None:intergenic |
| TTGATGACTCATGATGTTTG+TGG | 0.473686 | 1.1:+28461136 | MS.gene073948:CDS |
| TTCACCGGATAACCGTGCTT+TGG | 0.476151 | 1.1:+28468154 | MS.gene073948:CDS |
| GATTCTCACACTTGTACTGC+TGG | 0.482639 | 1.1:+28462579 | MS.gene073948:CDS |
| CATCGCTTGTGAATATATAG+TGG | 0.485083 | 1.1:-28462184 | None:intergenic |
| GTGTATAGTCTTCCTGTCCC+TGG | 0.486113 | 1.1:+28468814 | MS.gene073948:CDS |
| TATGTTGCAGCCTCATTCAC+CGG | 0.492821 | 1.1:+28468139 | MS.gene073948:intron |
| TCACAGATATTTGAAGAAGC+TGG | 0.493068 | 1.1:+28468856 | MS.gene073948:CDS |
| ACTTTGAGATTTGTAATGGA+TGG | 0.496494 | 1.1:+28463717 | MS.gene073948:CDS |
| TGCAGACCTTCTGTGTTGCA+GGG | 0.496669 | 1.1:-28468667 | None:intergenic |
| AAAGATGCTAGGAAAGAAGC+AGG | 0.500343 | 1.1:-28468969 | None:intergenic |
| CTTGTGAATATATAGTGGTC+AGG | 0.502316 | 1.1:-28462179 | None:intergenic |
| AATGGAATTTCAGGTTTGGA+TGG | 0.508384 | 1.1:+28468789 | MS.gene073948:intron |
| GGAGGTGGAAGGAACAAGAG+AGG | 0.512338 | 1.1:-28460678 | None:intergenic |
| GGAGTTTGGTCAGGATGCTA+AGG | 0.513184 | 1.1:+28461186 | MS.gene073948:CDS |
| GCCAAGTTATTTGCTTGCCA+AGG | 0.519501 | 1.1:+28463746 | MS.gene073948:CDS |
| GTAGGTTTGGGATCGTGAAA+AGG | 0.519967 | 1.1:+28462145 | MS.gene073948:intron |
| GGGATTGGTAATACTGATGC+CGG | 0.521210 | 1.1:+28462624 | MS.gene073948:CDS |
| TTGTGACATCGTTGAGAAGA+TGG | 0.523423 | 1.1:-28460840 | None:intergenic |
| TGCCCGCATGAATGAACCTC+AGG | 0.527832 | 1.1:+28468939 | MS.gene073948:CDS |
| TACTTGCAGAATCAAATCCT+TGG | 0.528971 | 1.1:-28463763 | None:intergenic |
| CAAACTGATCCAGTTGAAGA+AGG | 0.529297 | 1.1:-28460921 | None:intergenic |
| TGCACTCTCTTGCCAAAGCA+CGG | 0.532465 | 1.1:-28468166 | None:intergenic |
| ATGGCGAAAGCTTCTGAGAA+GGG | 0.536512 | 1.1:+28461070 | MS.gene073948:CDS |
| CTTGGTATGGAGGAAGGAGG+TGG | 0.539951 | 1.1:-28460693 | None:intergenic |
| AGTTGTGGTGCTTGTTTGGG+TGG | 0.540867 | 1.1:+28468901 | MS.gene073948:CDS |
| GCACCACAACTAGGACTAGC+AGG | 0.542395 | 1.1:-28468889 | None:intergenic |
| TCCTTGGCAAGCAAATAACT+TGG | 0.546758 | 1.1:-28463747 | None:intergenic |
| TGCCTGAGGTTCATTCATGC+GGG | 0.549238 | 1.1:-28468941 | None:intergenic |
| ATTGGAGCCACTGGTTGCCA+AGG | 0.549517 | 1.1:+28468030 | MS.gene073948:CDS |
| ATATGATGCATACTGATGCT+AGG | 0.550605 | 1.1:-28468271 | None:intergenic |
| TATATAGTGACCAGCAAGCA+AGG | 0.550703 | 1.1:+28467438 | MS.gene073948:CDS |
| AGTATACATAGGATCTTGTA+CGG | 0.554141 | 1.1:+28468208 | MS.gene073948:CDS |
| CCCAAACAAGCACCACAACT+AGG | 0.554453 | 1.1:-28468898 | None:intergenic |
| TGGTGCAACTTACAAATCAA+TGG | 0.554753 | 1.1:+28463895 | MS.gene073948:CDS |
| CTTCCTGTCCCTGGATCTGG+TGG | 0.555351 | 1.1:+28468823 | MS.gene073948:CDS |
| GAGAAGGGACATGTTACACC+TGG | 0.556267 | 1.1:+28461085 | MS.gene073948:CDS |
| TGCTTTGGCAAGAGAGTGCA+AGG | 0.558334 | 1.1:+28468169 | MS.gene073948:CDS |
| GTTGAAGCTGGAGGAAAGAA+TGG | 0.560027 | 1.1:+28464198 | MS.gene073948:CDS |
| ATTACAAATCTCAAAGTTGG+AGG | 0.561995 | 1.1:-28463711 | None:intergenic |
| GCACGGTTATCCGGTGAATG+AGG | 0.562486 | 1.1:-28468149 | None:intergenic |
| TCTTGTCCCTGCAACACAGA+AGG | 0.562826 | 1.1:+28468661 | MS.gene073948:CDS |
| TGTCTCAAAATTGGAGCCAC+TGG | 0.563417 | 1.1:+28468021 | MS.gene073948:CDS |
| TTGCCTGAGGTTCATTCATG+CGG | 0.565671 | 1.1:-28468942 | None:intergenic |
| ATTGGTGAAATAAGTGTGGC+TGG | 0.569430 | 1.1:+28463875 | MS.gene073948:CDS |
| CATTTGGTCAATTTGCAACA+GGG | 0.572263 | 1.1:+28462604 | MS.gene073948:CDS |
| CACATCGCTCTTGCTCAGGA+AGG | 0.575169 | 1.1:+28462430 | MS.gene073948:CDS |
| AGTACTTGATATCTTGCTCA+TGG | 0.576727 | 1.1:-28462247 | None:intergenic |
| GTATGGAGGAAGGAGGTGGA+AGG | 0.580431 | 1.1:-28460689 | None:intergenic |
| CATTACATATAAATATCTTG+AGG | 0.587292 | 1.1:+28464239 | MS.gene073948:CDS |
| AGGTCATTGCAGGCCAGGAG+AGG | 0.593257 | 1.1:+28462450 | MS.gene073948:CDS |
| TTACCACCAGATCCAGGGAC+AGG | 0.600757 | 1.1:-28468826 | None:intergenic |
| AATGGCGAAAGCTTCTGAGA+AGG | 0.607979 | 1.1:+28461069 | MS.gene073948:CDS |
| AAAATTGACAGAGTATACAT+AGG | 0.608193 | 1.1:+28468197 | MS.gene073948:CDS |
| TCAACCCAAATATTATCCCC+AGG | 0.614366 | 1.1:-28461103 | None:intergenic |
| TACATAGGATCTTGTACGGG+TGG | 0.615320 | 1.1:+28468212 | MS.gene073948:CDS |
| GGTTGACGCTGTGTCTGTTG+AGG | 0.615350 | 1.1:-28460900 | None:intergenic |
| CACCTCCTTCCTCCATACCA+AGG | 0.618957 | 1.1:+28460694 | MS.gene073948:CDS |
| AAGCACCTTGGTATGGAGGA+AGG | 0.621886 | 1.1:-28460699 | None:intergenic |
| CAGGTAAATCCAGATTACAA+AGG | 0.639525 | 1.1:+28462400 | MS.gene073948:intron |
| AAGTCTTACCACCAGATCCA+GGG | 0.642020 | 1.1:-28468831 | None:intergenic |
| AGAAGGGACATGTTACACCT+GGG | 0.642628 | 1.1:+28461086 | MS.gene073948:CDS |
| GTATACATAGGATCTTGTAC+GGG | 0.648403 | 1.1:+28468209 | MS.gene073948:CDS |
| TGTTGCAGGGACAAGAAATG+TGG | 0.650698 | 1.1:-28468654 | None:intergenic |
| GGACTAACCTTGGCAACCAG+TGG | 0.655420 | 1.1:-28468037 | None:intergenic |
| TTATATGTAATGCTATCTGG+AGG | 0.666348 | 1.1:-28464228 | None:intergenic |
| ACACCTGCTAGTCCTAGTTG+TGG | 0.669604 | 1.1:+28468886 | MS.gene073948:CDS |
| CTTCCTGAGCAAGAGCGATG+TGG | 0.677611 | 1.1:-28462429 | None:intergenic |
| CACCTTGGTATGGAGGAAGG+AGG | 0.682754 | 1.1:-28460696 | None:intergenic |
| AAGATGCTAGGAAAGAAGCA+GGG | 0.687159 | 1.1:-28468968 | None:intergenic |
| ACGGATGACATTGTGCAACA+TGG | 0.689455 | 1.1:+28464173 | MS.gene073948:CDS |
| GTTGCAGGGACAAGAAATGT+GGG | 0.707328 | 1.1:-28468653 | None:intergenic |
| GAAGGGACATGTTACACCTG+GGG | 0.712447 | 1.1:+28461087 | MS.gene073948:CDS |
| AGAAGCAGGGAACTTGCCTG+AGG | 0.716781 | 1.1:-28468955 | None:intergenic |
| GATTATTGGTGAAATAAGTG+TGG | 0.724786 | 1.1:+28463871 | MS.gene073948:intron |
| AACATGGTTGTTGAAGCTGG+AGG | 0.764525 | 1.1:+28464189 | MS.gene073948:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 28460671 | 28468984 | 28460671 | ID=MS.gene073948 |
| chr1.1 | mRNA | 28460671 | 28468984 | 28460671 | ID=MS.gene073948.t1;Parent=MS.gene073948 |
| chr1.1 | exon | 28460671 | 28460715 | 28460671 | ID=MS.gene073948.t1.exon1;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28460671 | 28460715 | 28460671 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28460810 | 28460938 | 28460810 | ID=MS.gene073948.t1.exon2;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28460810 | 28460938 | 28460810 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28461037 | 28461207 | 28461037 | ID=MS.gene073948.t1.exon3;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28461037 | 28461207 | 28461037 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28462149 | 28462301 | 28462149 | ID=MS.gene073948.t1.exon4;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28462149 | 28462301 | 28462149 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28462403 | 28462471 | 28462403 | ID=MS.gene073948.t1.exon5;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28462403 | 28462471 | 28462403 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28462564 | 28462680 | 28462564 | ID=MS.gene073948.t1.exon6;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28462564 | 28462680 | 28462564 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28463708 | 28463782 | 28463708 | ID=MS.gene073948.t1.exon7;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28463708 | 28463782 | 28463708 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28463872 | 28463949 | 28463872 | ID=MS.gene073948.t1.exon8;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28463872 | 28463949 | 28463872 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28464165 | 28464260 | 28464165 | ID=MS.gene073948.t1.exon9;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28464165 | 28464260 | 28464165 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28467410 | 28467459 | 28467410 | ID=MS.gene073948.t1.exon10;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28467410 | 28467459 | 28467410 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28467997 | 28468051 | 28467997 | ID=MS.gene073948.t1.exon11;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28467997 | 28468051 | 28467997 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28468149 | 28468280 | 28468149 | ID=MS.gene073948.t1.exon12;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28468149 | 28468280 | 28468149 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28468635 | 28468682 | 28468635 | ID=MS.gene073948.t1.exon13;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28468635 | 28468682 | 28468635 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| chr1.1 | exon | 28468802 | 28468984 | 28468802 | ID=MS.gene073948.t1.exon14;Parent=MS.gene073948.t1 |
| chr1.1 | CDS | 28468802 | 28468984 | 28468802 | ID=cds.MS.gene073948.t1;Parent=MS.gene073948.t1 |
| Gene Sequence |
| Protein sequence |
