Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074593.t1 | XP_003629390.1 | 96.1 | 154 | 6 | 0 | 22 | 175 | 7 | 160 | 2.50E-80 | 308.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074593.t1 | P17093 | 90.9 | 154 | 13 | 1 | 22 | 175 | 7 | 159 | 3.8e-77 | 288.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074593.t1 | B7FGL2 | 96.1 | 154 | 6 | 0 | 22 | 175 | 7 | 160 | 1.8e-80 | 308.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene074593 | 0.807955 | 3.92E-50 | -1.69E-46 |
| MS.gene050290 | MS.gene074593 | 0.854374 | 1.28E-61 | -1.69E-46 |
| MS.gene051904 | MS.gene074593 | 0.858044 | 1.08E-62 | -1.69E-46 |
| MS.gene054937 | MS.gene074593 | 0.803862 | 2.85E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 130 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTGAAAAATAATATCAA+TGG | - | 57563:1167-1186 | MS.gene074593:intergenic | 10.0% |
| !! | ATAAAAAAAAAAACAGAAAT+TGG | - | 57563:1262-1281 | MS.gene074593:intergenic | 10.0% |
| !! | TAAAAAAAAAAACAGAAATT+GGG | - | 57563:1261-1280 | MS.gene074593:intergenic | 10.0% |
| !! | TAAAAAAAAAATGTAGTCTT+TGG | + | 57563:1626-1645 | MS.gene074593:intron | 15.0% |
| !! | TGTTTGTAAATAAATAATAG+TGG | + | 57563:1959-1978 | MS.gene074593:intron | 15.0% |
| !!! | AAAGACTACATTTTTTTTTT+AGG | - | 57563:1627-1646 | MS.gene074593:intergenic | 15.0% |
| !! | TAAAGCAAAATTGAGTTAAA+TGG | + | 57563:1680-1699 | MS.gene074593:intron | 20.0% |
| !! | TAAGTGTGCAAATAAAAAAT+GGG | - | 57563:2709-2728 | MS.gene074593:intergenic | 20.0% |
| !! | TATTTATTTACAAACACAAC+AGG | - | 57563:1955-1974 | MS.gene074593:intergenic | 20.0% |
| !! | TGTCTGTTTATATGATAAAT+TGG | + | 57563:1925-1944 | MS.gene074593:intron | 20.0% |
| !!! | ATTTATGTATGTTTTGATAC+TGG | + | 57563:1802-1821 | MS.gene074593:intron | 20.0% |
| !!! | GAAGGTAATGTTTAATTTTA+GGG | + | 57563:1431-1450 | MS.gene074593:intron | 20.0% |
| !!! | GGCATTTTTTTTTATAAAAC+AGG | + | 57563:2512-2531 | MS.gene074593:intron | 20.0% |
| !!! | TATGTTCTAAACTTACATAT+AGG | + | 57563:1466-1485 | MS.gene074593:intron | 20.0% |
| !!! | TGAAGGTAATGTTTAATTTT+AGG | + | 57563:1430-1449 | MS.gene074593:intron | 20.0% |
| ! | AAACTTACATATAGGAATGT+AGG | + | 57563:1474-1493 | MS.gene074593:intron | 25.0% |
| ! | AAATTAAACATTACCTTCAG+TGG | - | 57563:1429-1448 | MS.gene074593:intergenic | 25.0% |
| ! | AACTAAAACAAATACACTCA+AGG | - | 57563:1518-1537 | MS.gene074593:intergenic | 25.0% |
| ! | AACTTACATATAGGAATGTA+GGG | + | 57563:1475-1494 | MS.gene074593:intron | 25.0% |
| ! | AAGTGTGCAAATAAAAAATG+GGG | - | 57563:2708-2727 | MS.gene074593:intergenic | 25.0% |
| ! | AATTTCACTTTCAAAGTAAC+TGG | - | 57563:2255-2274 | MS.gene074593:intergenic | 25.0% |
| ! | ATTGAATTCCTTTCATACTA+TGG | + | 57563:2681-2700 | MS.gene074593:intron | 25.0% |
| ! | CTAAGTGTGCAAATAAAAAA+TGG | - | 57563:2710-2729 | MS.gene074593:intergenic | 25.0% |
| ! | GTTATGGTTATGTTATGTTA+CGG | + | 57563:2109-2128 | MS.gene074593:intron | 25.0% |
| ! | TTAGATAAGGATAGAAACAT+TGG | + | 57563:1720-1739 | MS.gene074593:intron | 25.0% |
| ! | TTCTATAGAAAGCAAAAGAA+AGG | - | 57563:2886-2905 | MS.gene074593:intergenic | 25.0% |
| !! | GTATTGAGACATGTAATTTT+TGG | + | 57563:2087-2106 | MS.gene074593:intron | 25.0% |
| !!! | ACTTTATTAAGAAGTATCAG+AGG | + | 57563:2657-2676 | MS.gene074593:CDS | 25.0% |
| !!! | AGACATGTAATTTTTGGTTA+TGG | + | 57563:2093-2112 | MS.gene074593:intron | 25.0% |
| !!! | ATTTTAGTCCTCAAATGTAT+TGG | + | 57563:1542-1561 | MS.gene074593:intron | 25.0% |
| !!! | TTCTGTTTCTAATACAGAAA+TGG | + | 57563:2491-2510 | MS.gene074593:intron | 25.0% |
| ACACTTGCAAGACATAATAT+AGG | - | 57563:2182-2201 | MS.gene074593:intergenic | 30.0% | |
| ACAGAGGAGTAACAAAAAAA+GGG | - | 57563:2749-2768 | MS.gene074593:intergenic | 30.0% | |
| ACATACCATAGAAAACACTT+TGG | - | 57563:1226-1245 | MS.gene074593:intergenic | 30.0% | |
| ATGCAAAGCACAATTAGATA+AGG | + | 57563:1707-1726 | MS.gene074593:intron | 30.0% | |
| ATGTTATTATTGGACAATGC+AGG | + | 57563:2841-2860 | MS.gene074593:CDS | 30.0% | |
| CATACCATAGAAAACACTTT+GGG | - | 57563:1225-1244 | MS.gene074593:intergenic | 30.0% | |
| TAGAGTAGTTGCACAAATAA+AGG | - | 57563:2964-2983 | MS.gene074593:intergenic | 30.0% | |
| TATAGACCAATTCTTTCAAG+CGG | - | 57563:2138-2157 | MS.gene074593:intergenic | 30.0% | |
| TCCTCAAATGTATTGGATTT+GGG | + | 57563:1549-1568 | MS.gene074593:intron | 30.0% | |
| TGAATAGGACTATTATTGTC+AGG | + | 57563:2621-2640 | MS.gene074593:CDS | 30.0% | |
| TGCATTGTCCAATAATAACA+TGG | - | 57563:2842-2861 | MS.gene074593:intergenic | 30.0% | |
| TTTATTTGTGCAACTACTCT+AGG | + | 57563:2963-2982 | MS.gene074593:intron | 30.0% | |
| ! | ATTGTTAATAGCTCTGTGTA+TGG | + | 57563:2296-2315 | MS.gene074593:intron | 30.0% |
| ! | CGCAATACTTATTCTAGTTA+TGG | + | 57563:2002-2021 | MS.gene074593:intron | 30.0% |
| ! | TTGTTAATAGCTCTGTGTAT+GGG | + | 57563:2297-2316 | MS.gene074593:intron | 30.0% |
| AATGGAAGATCCAAAGATAC+AGG | - | 57563:1149-1168 | MS.gene074593:intergenic | 35.0% | |
| AGTTCGAAGAAATCTGGTAA+GGG | + | 57563:1332-1351 | MS.gene074593:intron | 35.0% | |
| ATAGGACTATTATTGTCAGG+AGG | + | 57563:2624-2643 | MS.gene074593:CDS | 35.0% | |
| CCTCAAATGTATTGGATTTG+GGG | + | 57563:1550-1569 | MS.gene074593:intron | 35.0% | |
| GAAGGAGACCATGTTATTAT+TGG | + | 57563:2831-2850 | MS.gene074593:CDS | 35.0% | |
| GACAGAGGAGTAACAAAAAA+AGG | - | 57563:2750-2769 | MS.gene074593:intergenic | 35.0% | |
| GTCCTCAAATGTATTGGATT+TGG | + | 57563:1548-1567 | MS.gene074593:intron | 35.0% | |
| TTCTTTGTTACAGACTGAGA+AGG | + | 57563:1185-1204 | MS.gene074593:intron | 35.0% | |
| ! | AATAGCTCTGTGTATGGTTA+CGG | + | 57563:2459-2478 | MS.gene074593:intron | 35.0% |
| ! | AATCGTTTCTGGAAATCAGT+TGG | + | 57563:1377-1396 | MS.gene074593:CDS | 35.0% |
| ! | TATGTCTTGCAAGTGTTGTT+GGG | + | 57563:2186-2205 | MS.gene074593:intron | 35.0% |
| ! | TGTAGGGATAAACAGCATTT+TGG | + | 57563:1491-1510 | MS.gene074593:intron | 35.0% |
| ! | TTATGTCTTGCAAGTGTTGT+TGG | + | 57563:2185-2204 | MS.gene074593:intron | 35.0% |
| ! | TTTTTGTTACTCCTCTGTCT+AGG | + | 57563:2751-2770 | MS.gene074593:intron | 35.0% |
| !!! | ACGCTATTTCTTTTGCATAG+TGG | + | 57563:1759-1778 | MS.gene074593:intron | 35.0% |
| AATGGGGACCATAGTATGAA+AGG | - | 57563:2692-2711 | MS.gene074593:intergenic | 40.0% | |
| AGATACAGGAGATAGAACGA+AGG | - | 57563:1135-1154 | MS.gene074593:intergenic | 40.0% | |
| AGATTCCTCTTGGCAATATC+AGG | + | 57563:1860-1879 | MS.gene074593:intron | 40.0% | |
| CAATGCAGGTGAGACTTTAT+AGG | + | 57563:2855-2874 | MS.gene074593:intron | 40.0% | |
| CAGTTCGAAGAAATCTGGTA+AGG | + | 57563:1331-1350 | MS.gene074593:intron | 40.0% | |
| CCCCAAATCCAATACATTTG+AGG | - | 57563:1553-1572 | MS.gene074593:intergenic | 40.0% | |
| CGTTCTATCTCCTGTATCTT+TGG | + | 57563:1136-1155 | MS.gene074593:intron | 40.0% | |
| GAAAAGGTGGAAATCGTTTC+TGG | + | 57563:1366-1385 | MS.gene074593:CDS | 40.0% | |
| GTTCGAAGAAATCTGGTAAG+GGG | + | 57563:1333-1352 | MS.gene074593:intron | 40.0% | |
| TAATAGAGCCTCACATTCGA+GGG | + | 57563:1584-1603 | MS.gene074593:intron | 40.0% | |
| TCCTATTCATCTTAGCACTG+TGG | - | 57563:2610-2629 | MS.gene074593:intergenic | 40.0% | |
| TGGACACTTCTTGTCAATGT+AGG | - | 57563:2540-2559 | MS.gene074593:intergenic | 40.0% | |
| TGTTGCAGTTCGAAGAAATC+TGG | + | 57563:1326-1345 | MS.gene074593:intron | 40.0% | |
| ! | AAACGATTTCCACCTTTTCC+AGG | - | 57563:1365-1384 | MS.gene074593:intergenic | 40.0% |
| ! | ATAGCTCTGTGTATGGGTTA+CGG | + | 57563:2303-2322 | MS.gene074593:intron | 40.0% |
| ! | TGTTTGTCTGAGATTCCTCT+TGG | + | 57563:1850-1869 | MS.gene074593:intron | 40.0% |
| ! | TTCTGGAAATCAGTTGGTCT+TGG | + | 57563:1383-1402 | MS.gene074593:CDS | 40.0% |
| !! | GCAACCCAAAGTGTTTTCTA+TGG | + | 57563:1218-1237 | MS.gene074593:CDS | 40.0% |
| AGAAATCTGGTAAGGGGAAG+AGG | + | 57563:1339-1358 | MS.gene074593:CDS | 45.0% | |
| ATCACCTTGCTTCCGTGTTA+AGG | + | 57563:2809-2828 | MS.gene074593:CDS | 45.0% | |
| ATCTAGTGGTGCAGGAAAGA+AGG | + | 57563:3039-3058 | MS.gene074593:CDS | 45.0% | |
| ATGGAAACATTGCCGGTGAA+TGG | - | 57563:2560-2579 | MS.gene074593:intergenic | 45.0% | |
| ATTACCTTCAGTGGCTTCTC+TGG | - | 57563:1420-1439 | MS.gene074593:intergenic | 45.0% | |
| CATGGTCTCCTTCCTTAACA+CGG | - | 57563:2824-2843 | MS.gene074593:intergenic | 45.0% | |
| CGGTATCCGCTTGAAAGAAT+TGG | + | 57563:2129-2148 | MS.gene074593:intron | 45.0% | |
| CTCTGTCTAGGTATGAGAAG+AGG | + | 57563:2763-2782 | MS.gene074593:intron | 45.0% | |
| CTCTTCTCATACCTAGACAG+AGG | - | 57563:2765-2784 | MS.gene074593:intergenic | 45.0% | |
| GCCACAGTGCTAAGATGAAT+AGG | + | 57563:2606-2625 | MS.gene074593:CDS | 45.0% | |
| GTAATAGAGCCTCACATTCG+AGG | + | 57563:1583-1602 | MS.gene074593:intron | 45.0% | |
| GTTGCTTACTTGTTCAGCCA+TGG | - | 57563:1101-1120 | MS.gene074593:intergenic | 45.0% | |
| TTACCTTCAGTGGCTTCTCT+GGG | - | 57563:1419-1438 | MS.gene074593:intergenic | 45.0% | |
| TTCCTCTTGGCAATATCAGG+TGG | + | 57563:1863-1882 | MS.gene074593:intron | 45.0% | |
| ! | GCAGGAAAGAAGGCTTTTAC+TGG | + | 57563:3049-3068 | MS.gene074593:CDS | 45.0% |
| ! | TCTTGCAAGTGTTGTTGGGA+AGG | + | 57563:2190-2209 | MS.gene074593:intron | 45.0% |
| !! | CTGAGCGTCTAGCTTTGTAT+TGG | + | 57563:1825-1844 | MS.gene074593:intron | 45.0% |
| !! | GACAAGAAGTGTCCATTCAC+CGG | + | 57563:2545-2564 | MS.gene074593:CDS | 45.0% |
| AACCTCACAGTCTTGGAGAG+TGG | - | 57563:2989-3008 | MS.gene074592:intergenic | 50.0% | |
| AAGCAAGGTGATACGTGAGC+GGG | - | 57563:2801-2820 | MS.gene074592:intergenic | 50.0% | |
| AGCCACCTGATATTGCCAAG+AGG | - | 57563:1868-1887 | MS.gene074593:intergenic | 50.0% | |
| ATCCCAGCTGGATCATCTAG+TGG | + | 57563:3025-3044 | MS.gene074593:CDS | 50.0% | |
| CACCACTAGATGATCCAGCT+GGG | - | 57563:3030-3049 | MS.gene074593:intergenic | 50.0% | |
| CACGTTGAACCTCACAGTCT+TGG | - | 57563:2996-3015 | MS.gene074593:intergenic | 50.0% | |
| CCTTCCTTAACACGGAAGCA+AGG | - | 57563:2816-2835 | MS.gene074593:intergenic | 50.0% | |
| CCTTGCTTCCGTGTTAAGGA+AGG | + | 57563:2813-2832 | MS.gene074593:CDS | 50.0% | |
| CTTAGCACTGTGGCAAGTTC+CGG | - | 57563:2600-2619 | MS.gene074593:intergenic | 50.0% | |
| GAAGCAAGGTGATACGTGAG+CGG | - | 57563:2802-2821 | MS.gene074593:intergenic | 50.0% | |
| GCTTACTTGTTCAGCCATGG+TGG | - | 57563:1098-1117 | MS.gene074593:intergenic | 50.0% | |
| GTGTTGAAAGTGATCCCAGC+TGG | + | 57563:3013-3032 | MS.gene074593:CDS | 50.0% | |
| TACCTTCAGTGGCTTCTCTG+GGG | - | 57563:1418-1437 | MS.gene074593:intergenic | 50.0% | |
| TACTTGTTCAGCCATGGTGG+TGG | - | 57563:1095-1114 | MS.gene074593:intergenic | 50.0% | |
| TCACCGGCAATGTTTCCATC+CGG | + | 57563:2561-2580 | MS.gene074593:CDS | 50.0% | |
| TTATGCCTCCCTCGAATGTG+AGG | - | 57563:1595-1614 | MS.gene074593:intergenic | 50.0% | |
| AAGGGGAAGAGGCCTGGAAA+AGG | + | 57563:1350-1369 | MS.gene074593:CDS | 55.0% | |
| ACCCCGGATGGAAACATTGC+CGG | - | 57563:2567-2586 | MS.gene074593:intergenic | 55.0% | |
| ACCGGCAATGTTTCCATCCG+GGG | + | 57563:2563-2582 | MS.gene074593:CDS | 55.0% | |
| ACCTTCAGTGGCTTCTCTGG+GGG | - | 57563:1417-1436 | MS.gene074593:intergenic | 55.0% | |
| ATCCGGGGTCGTATCTTAGC+CGG | + | 57563:2578-2597 | MS.gene074593:CDS | 55.0% | |
| CACCGGCAATGTTTCCATCC+GGG | + | 57563:2562-2581 | MS.gene074593:CDS | 55.0% | |
| GCACCACTAGATGATCCAGC+TGG | - | 57563:3031-3050 | MS.gene074593:intergenic | 55.0% | |
| GCTGGATCATCTAGTGGTGC+AGG | + | 57563:3031-3050 | MS.gene074593:CDS | 55.0% | |
| TAGAGCCTCACATTCGAGGG+AGG | + | 57563:1587-1606 | MS.gene074593:intron | 55.0% | |
| TTCCGGCTAAGATACGACCC+CGG | - | 57563:2583-2602 | MS.gene074593:intergenic | 55.0% | |
| ACCCCCAGAGAAGCCACTGA+AGG | + | 57563:1413-1432 | MS.gene074593:CDS | 60.0% | |
| CCTCTGCAACTCCACCACCA+TGG | + | 57563:1081-1100 | MS.gene074593:CDS | 60.0% | |
| GGCCACTCTCCAAGACTGTG+AGG | + | 57563:2984-3003 | MS.gene074593:intron | 60.0% | |
| GGCTAAGATACGACCCCGGA+TGG | - | 57563:2579-2598 | MS.gene074593:intergenic | 60.0% | |
| GGGAAGAGGCCTGGAAAAGG+TGG | + | 57563:1353-1372 | MS.gene074593:CDS | 60.0% | |
| TCTGGTAAGGGGAAGAGGCC+TGG | + | 57563:1344-1363 | MS.gene074593:CDS | 60.0% | |
| !! | CCATGGTGGTGGAGTTGCAG+AGG | - | 57563:1084-1103 | MS.gene074593:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57563 | gene | 1055 | 3078 | 1055 | ID=MS.gene074593 |
| 57563 | mRNA | 1055 | 3078 | 1055 | ID=MS.gene074593.t1;Parent=MS.gene074593 |
| 57563 | exon | 1055 | 1111 | 1055 | ID=MS.gene074593.t1.exon1;Parent=MS.gene074593.t1 |
| 57563 | CDS | 1055 | 1111 | 1055 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| 57563 | exon | 1198 | 1239 | 1198 | ID=MS.gene074593.t1.exon2;Parent=MS.gene074593.t1 |
| 57563 | CDS | 1198 | 1239 | 1198 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| 57563 | exon | 1334 | 1434 | 1334 | ID=MS.gene074593.t1.exon3;Parent=MS.gene074593.t1 |
| 57563 | CDS | 1334 | 1434 | 1334 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| 57563 | exon | 2534 | 2678 | 2534 | ID=MS.gene074593.t1.exon4;Parent=MS.gene074593.t1 |
| 57563 | CDS | 2534 | 2678 | 2534 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| 57563 | exon | 2773 | 2862 | 2773 | ID=MS.gene074593.t1.exon5;Parent=MS.gene074593.t1 |
| 57563 | CDS | 2773 | 2862 | 2773 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| 57563 | exon | 2985 | 3078 | 2985 | ID=MS.gene074593.t1.exon6;Parent=MS.gene074593.t1 |
| 57563 | CDS | 2985 | 3078 | 2985 | ID=cds.MS.gene074593.t1;Parent=MS.gene074593.t1 |
| Gene Sequence |
| Protein sequence |
