AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07880


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07880.t1 MTR_4g130770 94.086 186 8 1 1 186 1 183 1.20e-123 362
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07880.t1 AT4G30600 69.613 181 49 2 1 178 1 178 2.03e-81 253
MS.gene07880.t1 AT4G30600 69.613 181 49 2 1 178 1 178 2.03e-81 253

Find 41 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTGGTCATGCAATGAAAT+TGG 0.219547 8.3:+70889557 MS.gene07880:CDS
TTATCACCTTTCAACACTTT+TGG 0.220142 8.3:-70890062 None:intergenic
CAAGAGGAGGATTAATCCTT+TGG 0.352909 8.3:+70889540 MS.gene07880:CDS
GGCAGTGCTGGTGGTGGCGG+CGG 0.376957 8.3:+70889980 MS.gene07880:CDS
AGTGCTGGTGGTGGCGGCGG+TGG 0.381229 8.3:+70889983 MS.gene07880:CDS
CTGTGGTTGTAGGTGGGAAT+AGG 0.413522 8.3:+70889909 MS.gene07880:CDS
AATGGCAGTGCTGGTGGTGG+CGG 0.423824 8.3:+70889977 MS.gene07880:CDS
AAATCGAATCCTGTGGTTGT+AGG 0.472230 8.3:+70889899 MS.gene07880:CDS
AATTCTGCATCTGCTTTATG+TGG 0.482797 8.3:+70889745 MS.gene07880:CDS
GAGCTGCTTATTCTCTCAAA+TGG 0.492519 8.3:+70889675 MS.gene07880:CDS
CTATTCCCACCTACAACCAC+AGG 0.514029 8.3:-70889908 None:intergenic
GAAATTGGTAACGCTTTGAA+AGG 0.518123 8.3:+70889572 MS.gene07880:CDS
GGATCTGACGGGAAGAAGAA+TGG 0.524281 8.3:+70889959 MS.gene07880:CDS
AAAATCTCGATACACAGTTC+TGG 0.526147 8.3:-70889819 None:intergenic
GGGAAGAAGAATGGCAGTGC+TGG 0.539935 8.3:+70889968 MS.gene07880:CDS
TCGAATCCTGTGGTTGTAGG+TGG 0.540946 8.3:+70889902 MS.gene07880:CDS
TGGAAGGGAGATGGATCTGA+CGG 0.542887 8.3:+70889947 MS.gene07880:CDS
CAATTTCATTGCATGACCAA+AGG 0.546136 8.3:-70889556 None:intergenic
GATTCGATCTTGTCTTCTAG+AGG 0.546611 8.3:+70889613 MS.gene07880:CDS
TGTCTTCTAGAGGAGCGATC+TGG 0.547527 8.3:+70889623 MS.gene07880:CDS
ATCTCGATACACAGTTCTGG+TGG 0.553576 8.3:-70889816 None:intergenic
CAAGTGACATGGAAGGGAGA+TGG 0.559417 8.3:+70889938 MS.gene07880:CDS
TTGAGAGAATAAGCAGCTCC+AGG 0.560375 8.3:-70889671 None:intergenic
GCAGTTGAAGATTGAAGCTG+AGG 0.577891 8.3:+70889856 MS.gene07880:CDS
TGAAGCTGAGGCACGTGCTG+AGG 0.588224 8.3:+70889868 MS.gene07880:CDS
GGCGGTGGTTTGAAGAATGA+TGG 0.588491 8.3:+70889998 MS.gene07880:CDS
TGTTAAGCGTGAATTCTCGC+AGG 0.597191 8.3:+70889784 MS.gene07880:CDS
CGAATCCTGTGGTTGTAGGT+GGG 0.598863 8.3:+70889903 MS.gene07880:CDS
ACCAACCCAAAAGTGTTGAA+AGG 0.600750 8.3:+70890056 MS.gene07880:CDS
GGAAGGGAGATGGATCTGAC+GGG 0.606947 8.3:+70889948 MS.gene07880:CDS
AAACAGCAAGTGACATGGAA+GGG 0.612227 8.3:+70889932 MS.gene07880:CDS
GATCGAATCAATGTATCGAT+CGG 0.615533 8.3:-70889599 None:intergenic
AAGAAGAATGGCAGTGCTGG+TGG 0.616387 8.3:+70889971 MS.gene07880:CDS
GTTTGAAGAATGATGGTGAT+GGG 0.620460 8.3:+70890005 MS.gene07880:CDS
AAGAATGGCAGTGCTGGTGG+TGG 0.622850 8.3:+70889974 MS.gene07880:CDS
TCTTACAATTACGATGCACC+TGG 0.626789 8.3:+70889653 MS.gene07880:CDS
GAAACAGCAAGTGACATGGA+AGG 0.631386 8.3:+70889931 MS.gene07880:CDS
TCTCGATACACAGTTCTGGT+GGG 0.644564 8.3:-70889815 None:intergenic
TTTGAAGAAATCGAATCCTG+TGG 0.647958 8.3:+70889892 MS.gene07880:CDS
ATAGGAAACAGCAAGTGACA+TGG 0.648538 8.3:+70889927 MS.gene07880:CDS
GGTTTGAAGAATGATGGTGA+TGG 0.671022 8.3:+70890004 MS.gene07880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATCTCGATACACAGTTC+TGG - chr8.3:70889822-70889841 None:intergenic 35.0%
AATTCTGCATCTGCTTTATG+TGG + chr8.3:70889745-70889764 MS.gene07880:CDS 35.0%
CAATTTCATTGCATGACCAA+AGG - chr8.3:70889559-70889578 None:intergenic 35.0%
CTTTGGTCATGCAATGAAAT+TGG + chr8.3:70889557-70889576 MS.gene07880:CDS 35.0%
TTTGAAGAAATCGAATCCTG+TGG + chr8.3:70889892-70889911 MS.gene07880:CDS 35.0%
! GATCGAATCAATGTATCGAT+CGG - chr8.3:70889602-70889621 None:intergenic 35.0%
! GTTTGAAGAATGATGGTGAT+GGG + chr8.3:70890005-70890024 MS.gene07880:CDS 35.0%
! TGGACTTTTCACAATGATCT+AGG + chr8.3:70889695-70889714 MS.gene07880:CDS 35.0%
!! AATGATGGTGATGGGAAAAA+TGG + chr8.3:70890013-70890032 MS.gene07880:CDS 35.0%
!! ATGATGGTGATGGGAAAAAT+GGG + chr8.3:70890014-70890033 MS.gene07880:CDS 35.0%
!! GAAATTGGTAACGCTTTGAA+AGG + chr8.3:70889572-70889591 MS.gene07880:CDS 35.0%
!!! CAGTTACTGATTTTCACAAG+AGG + chr8.3:70889524-70889543 MS.gene07880:CDS 35.0%
!!! TTACTGATTTTCACAAGAGG+AGG + chr8.3:70889527-70889546 MS.gene07880:CDS 35.0%
AAACAGCAAGTGACATGGAA+GGG + chr8.3:70889932-70889951 MS.gene07880:CDS 40.0%
AAATCGAATCCTGTGGTTGT+AGG + chr8.3:70889899-70889918 MS.gene07880:CDS 40.0%
ATAGGAAACAGCAAGTGACA+TGG + chr8.3:70889927-70889946 MS.gene07880:CDS 40.0%
CAAGAGGAGGATTAATCCTT+TGG + chr8.3:70889540-70889559 MS.gene07880:CDS 40.0%
GAGCTGCTTATTCTCTCAAA+TGG + chr8.3:70889675-70889694 MS.gene07880:CDS 40.0%
GATTCGATCTTGTCTTCTAG+AGG + chr8.3:70889613-70889632 MS.gene07880:CDS 40.0%
TCTTACAATTACGATGCACC+TGG + chr8.3:70889653-70889672 MS.gene07880:CDS 40.0%
! GGTTTGAAGAATGATGGTGA+TGG + chr8.3:70890004-70890023 MS.gene07880:CDS 40.0%
!! ACCAACCCAAAAGTGTTGAA+AGG + chr8.3:70890056-70890075 MS.gene07880:CDS 40.0%
!! ACCTTTCAACACTTTTGGGT+TGG - chr8.3:70890060-70890079 None:intergenic 40.0%
GAAACAGCAAGTGACATGGA+AGG + chr8.3:70889931-70889950 MS.gene07880:CDS 45.0%
GCAGTTGAAGATTGAAGCTG+AGG + chr8.3:70889856-70889875 MS.gene07880:CDS 45.0%
TGTTAAGCGTGAATTCTCGC+AGG + chr8.3:70889784-70889803 MS.gene07880:CDS 45.0%
TTGAGAGAATAAGCAGCTCC+AGG - chr8.3:70889674-70889693 None:intergenic 45.0%
! ATCTCGATACACAGTTCTGG+TGG - chr8.3:70889819-70889838 None:intergenic 45.0%
! TCTCGATACACAGTTCTGGT+GGG - chr8.3:70889818-70889837 None:intergenic 45.0%
CAAGTGACATGGAAGGGAGA+TGG + chr8.3:70889938-70889957 MS.gene07880:CDS 50.0%
CGAATCCTGTGGTTGTAGGT+GGG + chr8.3:70889903-70889922 MS.gene07880:CDS 50.0%
CTATTCCCACCTACAACCAC+AGG - chr8.3:70889911-70889930 None:intergenic 50.0%
CTGTGGTTGTAGGTGGGAAT+AGG + chr8.3:70889909-70889928 MS.gene07880:CDS 50.0%
GGATCTGACGGGAAGAAGAA+TGG + chr8.3:70889959-70889978 MS.gene07880:CDS 50.0%
GGCGGTGGTTTGAAGAATGA+TGG + chr8.3:70889998-70890017 MS.gene07880:CDS 50.0%
TCGAATCCTGTGGTTGTAGG+TGG + chr8.3:70889902-70889921 MS.gene07880:CDS 50.0%
TGGAAGGGAGATGGATCTGA+CGG + chr8.3:70889947-70889966 MS.gene07880:CDS 50.0%
! AAGAAGAATGGCAGTGCTGG+TGG + chr8.3:70889971-70889990 MS.gene07880:CDS 50.0%
! TGTCTTCTAGAGGAGCGATC+TGG + chr8.3:70889623-70889642 MS.gene07880:CDS 50.0%
GGAAGGGAGATGGATCTGAC+GGG + chr8.3:70889948-70889967 MS.gene07880:CDS 55.0%
GGGAAGAAGAATGGCAGTGC+TGG + chr8.3:70889968-70889987 MS.gene07880:CDS 55.0%
!! AAGAATGGCAGTGCTGGTGG+TGG + chr8.3:70889974-70889993 MS.gene07880:CDS 55.0%
TGAAGCTGAGGCACGTGCTG+AGG + chr8.3:70889868-70889887 MS.gene07880:CDS 60.0%
!! AATGGCAGTGCTGGTGGTGG+CGG + chr8.3:70889977-70889996 MS.gene07880:CDS 60.0%
!! AGTGCTGGTGGTGGCGGCGG+TGG + chr8.3:70889983-70890002 MS.gene07880:CDS 75.0%
!! GGCAGTGCTGGTGGTGGCGG+CGG + chr8.3:70889980-70889999 MS.gene07880:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 70889515 70890081 70889515 ID=MS.gene07880
chr8.3 mRNA 70889515 70890081 70889515 ID=MS.gene07880.t1;Parent=MS.gene07880
chr8.3 exon 70889515 70890081 70889515 ID=MS.gene07880.t1.exon1;Parent=MS.gene07880.t1
chr8.3 CDS 70889515 70890081 70889515 ID=cds.MS.gene07880.t1;Parent=MS.gene07880.t1
Gene Sequence

>MS.gene07880

ATGTTAGAGCAGTTACTGATTTTCACAAGAGGAGGATTAATCCTTTGGTCATGCAATGAAATTGGTAACGCTTTGAAAGGATCGCCGATCGATACATTGATTCGATCTTGTCTTCTAGAGGAGCGATCTGGTGCTGCATCTTACAATTACGATGCACCTGGAGCTGCTTATTCTCTCAAATGGACTTTTCACAATGATCTAGGTCTTGTTTTCGTTGCTGTTTATCAGAGAATTCTGCATCTGCTTTATGTGGATGATTTGCTTGCTGCTGTTAAGCGTGAATTCTCGCAGGTTTATGATCCCACCAGAACTGTGTATCGAGATTTTGATCAGATTTTTAAGCAGTTGAAGATTGAAGCTGAGGCACGTGCTGAGGATTTGAAGAAATCGAATCCTGTGGTTGTAGGTGGGAATAGGAAACAGCAAGTGACATGGAAGGGAGATGGATCTGACGGGAAGAAGAATGGCAGTGCTGGTGGTGGCGGCGGTGGTTTGAAGAATGATGGTGATGGGAAAAATGGGAAGAAGAATTCTGATAATGACCAACCCAAAAGTGTTGAAAGGTGA

Protein sequence

>MS.gene07880.t1

MLEQLLIFTRGGLILWSCNEIGNALKGSPIDTLIRSCLLEERSGAASYNYDAPGAAYSLKWTFHNDLGLVFVAVYQRILHLLYVDDLLAAVKREFSQVYDPTRTVYRDFDQIFKQLKIEAEARAEDLKKSNPVVVGGNRKQQVTWKGDGSDGKKNGSAGGGGGGLKNDGDGKNGKKNSDNDQPKSVER