AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08082


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08082.t1 MTR_3g061590 97.638 254 6 0 12 265 6 259 0.0 508
MS.gene08082.t1 MTR_8g073580 50.980 255 113 4 18 265 40 289 2.81e-74 228
MS.gene08082.t1 MTR_8g073580 53.110 209 87 3 64 265 38 242 2.17e-71 219
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08082.t1 AT3G20890 60.554 289 76 4 13 265 6 292 3.53e-108 315
MS.gene08082.t1 AT3G20890 57.635 203 53 1 96 265 27 229 3.01e-77 234
MS.gene08082.t1 AT5G66010 49.587 242 109 5 16 253 10 242 6.54e-74 226
MS.gene08082.t1 AT5G66010 48.630 146 70 2 112 253 1 145 1.30e-43 145

Find 74 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGTGAAGGCTTCTGTGTTT+TGG 0.182250 3.3:-49509608 MS.gene08082:CDS
ATCCACATTGTAATGAATTC+AGG 0.251651 3.3:-49509292 MS.gene08082:CDS
TCATCCTTATTAGCAGAAAA+TGG 0.345506 3.3:+49509358 None:intergenic
AGATGGATTGTACATATAAC+TGG 0.382208 3.3:+49509786 None:intergenic
ACTTTGCTCTTCAAAGAAAC+AGG 0.406141 3.3:-49509567 MS.gene08082:CDS
TGCTAATAAGGATGACATAA+TGG 0.410596 3.3:-49509350 MS.gene08082:CDS
TCCGTCTCACCATGCGTTGA+AGG 0.422533 3.3:+49509160 None:intergenic
CTACAAGGCAGTTGCAAATG+AGG 0.438613 3.3:-49509491 MS.gene08082:CDS
CGCAGTCGAACAACAGGAAA+TGG 0.442842 3.3:+49509730 None:intergenic
AGACCGCGCAGTCGAACAAC+AGG 0.447152 3.3:+49509724 None:intergenic
CTTCATCATATGATTTAGCT+CGG 0.461470 3.3:+49509428 None:intergenic
TCTTCATTCTATGATTCCCC+TGG 0.465549 3.3:-49509811 MS.gene08082:CDS
ACCATCTGGGGAGGCTTATG+TGG 0.470749 3.3:-49509263 MS.gene08082:CDS
ATATGATGAAGGAAAAGATT+CGG 0.489693 3.3:-49509416 MS.gene08082:CDS
CTTCAAGGAGTATGTGTTGT+CGG 0.500765 3.3:-49509323 MS.gene08082:CDS
CACAGGAGTGTTGCGACTGA+GGG 0.505697 3.3:-49509386 MS.gene08082:CDS
ATGTACAATCCATCTCCATA+TGG 0.508179 3.3:-49509778 MS.gene08082:CDS
ACAGGAAATGGTGGAGGAGG+AGG 0.509420 3.3:+49509742 None:intergenic
CTCGGGGAGCACTATGACGA+GGG 0.512824 3.3:+49509446 None:intergenic
TCCACATTGTAATGAATTCA+GGG 0.513953 3.3:-49509291 MS.gene08082:CDS
TTCATCATATGATTTAGCTC+GGG 0.517226 3.3:+49509429 None:intergenic
CGAGCTAAATCATATGATGA+AGG 0.518042 3.3:-49509427 MS.gene08082:CDS
ATCAGTTTCAGTGCAATCAA+AGG 0.523371 3.3:+49509699 None:intergenic
TGGTGGTGATGGGCGTGAAA+TGG 0.525727 3.3:-49509866 MS.gene08082:CDS
AAATTTGCTGATGGTGGTGA+TGG 0.527499 3.3:-49509877 MS.gene08082:CDS
CCGTCTCACCATGCGTTGAA+GGG 0.530469 3.3:+49509161 None:intergenic
TGACATAATGGAGTTCTTCA+AGG 0.535855 3.3:-49509338 MS.gene08082:CDS
CCCTTCAACGCATGGTGAGA+CGG 0.535909 3.3:-49509161 MS.gene08082:CDS
GATGTGGCCGAGTTCTTCCA+TGG 0.538399 3.3:-49509679 MS.gene08082:CDS
GCAAAGGATAGAATGACGCT+TGG 0.544787 3.3:-49509205 MS.gene08082:CDS
CGTCATTCTATCCTTTGCCA+TGG 0.545218 3.3:+49509210 None:intergenic
TCAGTTTCAGTGCAATCAAA+GGG 0.547953 3.3:+49509700 None:intergenic
CAAAGGATAGAATGACGCTT+GGG 0.551160 3.3:-49509204 MS.gene08082:CDS
GGTTGGCTGACATATCCATA+TGG 0.556296 3.3:+49509763 None:intergenic
CAAAGAAACAGGCAGAACAT+AGG 0.560049 3.3:-49509556 MS.gene08082:CDS
TTCAGTAAATTTGCTGATGG+TGG 0.563548 3.3:-49509883 MS.gene08082:intron
CTGACATATCCATATGGAGA+TGG 0.564910 3.3:+49509769 None:intergenic
TGTACATATAACTGGAACCA+GGG 0.571227 3.3:+49509794 None:intergenic
AGAGCAAAGTCAACTTGAAG+AGG 0.573046 3.3:+49509580 None:intergenic
ACAACAGGAAATGGTGGAGG+AGG 0.573282 3.3:+49509739 None:intergenic
TCCACATAAGCCTCCCCAGA+TGG 0.579302 3.3:+49509262 None:intergenic
GTCTAGACCATGGAAGAACT+CGG 0.581671 3.3:+49509672 None:intergenic
GAGCTATTCCCTTCAACGCA+TGG 0.586026 3.3:-49509169 MS.gene08082:CDS
CAAAACACAGAAGCCTTCAC+CGG 0.588242 3.3:+49509609 None:intergenic
TGATGATTCAAAAGCAGCCA+TGG 0.593984 3.3:-49509227 MS.gene08082:CDS
AATTTCAGTAAATTTGCTGA+TGG 0.594224 3.3:-49509886 MS.gene08082:intron
ACACAGGAGTGTTGCGACTG+AGG 0.596079 3.3:-49509387 MS.gene08082:CDS
TCATCATATGATTTAGCTCG+GGG 0.596516 3.3:+49509430 None:intergenic
TTCAAAAGCAGCCATGGCAA+AGG 0.597698 3.3:-49509221 MS.gene08082:CDS
CGAACAACAGGAAATGGTGG+AGG 0.604157 3.3:+49509736 None:intergenic
GAAATGGTGGAGGAGGAGGT+TGG 0.608351 3.3:+49509746 None:intergenic
TGATTGCACTGAAACTGATG+TGG 0.609327 3.3:-49509695 MS.gene08082:CDS
ACGGAAGATGCAATTTCAAG+AGG 0.618509 3.3:-49509142 MS.gene08082:CDS
AAAGATTCGGCTGAGCACAC+AGG 0.623512 3.3:-49509403 MS.gene08082:CDS
ACGTCAGCGGACAGTTGACG+CGG 0.625189 3.3:-49509836 MS.gene08082:CDS
AAGATGCAATTTCAAGAGGA+CGG 0.628738 3.3:-49509138 MS.gene08082:CDS
AATTTGCTGATGGTGGTGAT+GGG 0.635503 3.3:-49509876 MS.gene08082:CDS
TTTCCTGTTGTTCGACTGCG+CGG 0.636494 3.3:-49509727 MS.gene08082:CDS
TAAGAGGCAGGAATACTACA+AGG 0.645116 3.3:-49509506 MS.gene08082:CDS
GCTCGGGGAGCACTATGACG+AGG 0.653439 3.3:+49509445 None:intergenic
CAAGGAGTATGTGTTGTCGG+AGG 0.654683 3.3:-49509320 MS.gene08082:CDS
CCCCTGAATTCATTACAATG+TGG 0.658546 3.3:+49509290 None:intergenic
CGTCAGCGGACAGTTGACGC+GGG 0.660169 3.3:-49509835 MS.gene08082:CDS
TTGTACATATAACTGGAACC+AGG 0.660817 3.3:+49509793 None:intergenic
CATCAACTATGTCTAGACCA+TGG 0.660852 3.3:+49509662 None:intergenic
CACATTGTAATGAATTCAGG+GGG 0.663594 3.3:-49509289 MS.gene08082:CDS
AGTCGAACAACAGGAAATGG+TGG 0.675557 3.3:+49509733 None:intergenic
CCACATTGTAATGAATTCAG+GGG 0.677967 3.3:-49509290 MS.gene08082:CDS
ACAGGAGTGTTGCGACTGAG+GGG 0.684610 3.3:-49509385 MS.gene08082:CDS
TCGGGGAGCACTATGACGAG+GGG 0.689760 3.3:+49509447 None:intergenic
AATGAGGTTTCAGATGCTCG+AGG 0.692222 3.3:-49509475 MS.gene08082:CDS
AAATGGCTTCAAAACGTCAG+CGG 0.695070 3.3:-49509849 MS.gene08082:CDS
GAGGTTTCAGATGCTCGAGG+CGG 0.695093 3.3:-49509472 MS.gene08082:CDS
GTACATATAACTGGAACCAG+GGG 0.705619 3.3:+49509795 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATATGATGAAGGAAAAGATT+CGG - chr3.3:49509706-49509725 MS.gene08082:CDS 25.0%
!! ATTACCATTTTCTGCTAATA+AGG - chr3.3:49509760-49509779 MS.gene08082:CDS 25.0%
!!! AAGCTTATTGACTTCAATTT+TGG - chr3.3:49509202-49509221 MS.gene08082:CDS 25.0%
!!! AATTTCAGTAAATTTGCTGA+TGG - chr3.3:49509236-49509255 MS.gene08082:CDS 25.0%
ATCCACATTGTAATGAATTC+AGG - chr3.3:49509830-49509849 MS.gene08082:CDS 30.0%
CTTCATCATATGATTTAGCT+CGG + chr3.3:49509697-49509716 None:intergenic 30.0%
GACATAAGTTTAAGTACTCT+AGG + chr3.3:49509164-49509183 None:intergenic 30.0%
TCATCCTTATTAGCAGAAAA+TGG + chr3.3:49509767-49509786 None:intergenic 30.0%
TCCACATTGTAATGAATTCA+GGG - chr3.3:49509831-49509850 MS.gene08082:CDS 30.0%
TGCTAATAAGGATGACATAA+TGG - chr3.3:49509772-49509791 MS.gene08082:CDS 30.0%
! GATGTTCTTTTTGTTCACAA+AGG - chr3.3:49509479-49509498 MS.gene08082:CDS 30.0%
! TTCATCATATGATTTAGCTC+GGG + chr3.3:49509696-49509715 None:intergenic 30.0%
! TTGAAGTTTTCAGAAGTAAG+AGG - chr3.3:49509600-49509619 MS.gene08082:CDS 30.0%
!! AGATGGATTGTACATATAAC+TGG + chr3.3:49509339-49509358 None:intergenic 30.0%
AAGATGCAATTTCAAGAGGA+CGG - chr3.3:49509984-49510003 MS.gene08082:CDS 35.0%
ACTTTGCTCTTCAAAGAAAC+AGG - chr3.3:49509555-49509574 MS.gene08082:CDS 35.0%
ATCAGTTTCAGTGCAATCAA+AGG + chr3.3:49509426-49509445 None:intergenic 35.0%
ATGTACAATCCATCTCCATA+TGG - chr3.3:49509344-49509363 MS.gene08082:CDS 35.0%
CACATTGTAATGAATTCAGG+GGG - chr3.3:49509833-49509852 MS.gene08082:CDS 35.0%
CCACATTGTAATGAATTCAG+GGG - chr3.3:49509832-49509851 MS.gene08082:CDS 35.0%
CGAGCTAAATCATATGATGA+AGG - chr3.3:49509695-49509714 MS.gene08082:CDS 35.0%
TCAGTTTCAGTGCAATCAAA+GGG + chr3.3:49509425-49509444 None:intergenic 35.0%
TGTACATATAACTGGAACCA+GGG + chr3.3:49509331-49509350 None:intergenic 35.0%
TTGTACATATAACTGGAACC+AGG + chr3.3:49509332-49509351 None:intergenic 35.0%
! GTTCTTTTTGTTCACAAAGG+TGG - chr3.3:49509482-49509501 MS.gene08082:CDS 35.0%
! TCATCATATGATTTAGCTCG+GGG + chr3.3:49509695-49509714 None:intergenic 35.0%
! TGACATAATGGAGTTCTTCA+AGG - chr3.3:49509784-49509803 MS.gene08082:CDS 35.0%
!! TTCAGTAAATTTGCTGATGG+TGG - chr3.3:49509239-49509258 MS.gene08082:CDS 35.0%
AAATGGCTTCAAAACGTCAG+CGG - chr3.3:49509273-49509292 MS.gene08082:CDS 40.0%
ACGGAAGATGCAATTTCAAG+AGG - chr3.3:49509980-49509999 MS.gene08082:CDS 40.0%
AGAGCAAAGTCAACTTGAAG+AGG + chr3.3:49509545-49509564 None:intergenic 40.0%
CAAAGAAACAGGCAGAACAT+AGG - chr3.3:49509566-49509585 MS.gene08082:CDS 40.0%
CAAAGGATAGAATGACGCTT+GGG - chr3.3:49509918-49509937 MS.gene08082:intron 40.0%
CATCAACTATGTCTAGACCA+TGG + chr3.3:49509463-49509482 None:intergenic 40.0%
CCCCTGAATTCATTACAATG+TGG + chr3.3:49509835-49509854 None:intergenic 40.0%
CTGACATATCCATATGGAGA+TGG + chr3.3:49509356-49509375 None:intergenic 40.0%
GTACATATAACTGGAACCAG+GGG + chr3.3:49509330-49509349 None:intergenic 40.0%
TAAGAGGCAGGAATACTACA+AGG - chr3.3:49509616-49509635 MS.gene08082:CDS 40.0%
TCTTCATTCTATGATTCCCC+TGG - chr3.3:49509311-49509330 MS.gene08082:CDS 40.0%
TGATGATTCAAAAGCAGCCA+TGG - chr3.3:49509895-49509914 MS.gene08082:intron 40.0%
TGATTGCACTGAAACTGATG+TGG - chr3.3:49509427-49509446 MS.gene08082:CDS 40.0%
! AGTTTTCAGAAGTAAGAGGC+AGG - chr3.3:49509604-49509623 MS.gene08082:CDS 40.0%
! CTTCAAGGAGTATGTGTTGT+CGG - chr3.3:49509799-49509818 MS.gene08082:CDS 40.0%
!! AAATTTGCTGATGGTGGTGA+TGG - chr3.3:49509245-49509264 MS.gene08082:CDS 40.0%
!! AATTTGCTGATGGTGGTGAT+GGG - chr3.3:49509246-49509265 MS.gene08082:CDS 40.0%
AATGAGGTTTCAGATGCTCG+AGG - chr3.3:49509647-49509666 MS.gene08082:CDS 45.0%
AGTCGAACAACAGGAAATGG+TGG + chr3.3:49509392-49509411 None:intergenic 45.0%
CAAAACACAGAAGCCTTCAC+CGG + chr3.3:49509516-49509535 None:intergenic 45.0%
CGTCATTCTATCCTTTGCCA+TGG + chr3.3:49509915-49509934 None:intergenic 45.0%
CTACAAGGCAGTTGCAAATG+AGG - chr3.3:49509631-49509650 MS.gene08082:CDS 45.0%
GCAAAGGATAGAATGACGCT+TGG - chr3.3:49509917-49509936 MS.gene08082:intron 45.0%
GTCTAGACCATGGAAGAACT+CGG + chr3.3:49509453-49509472 None:intergenic 45.0%
TTCAAAAGCAGCCATGGCAA+AGG - chr3.3:49509901-49509920 MS.gene08082:intron 45.0%
! CACAAAGGTGGCAAGTTTTC+CGG - chr3.3:49509494-49509513 MS.gene08082:CDS 45.0%
! GGTGAAGGCTTCTGTGTTTT+GGG - chr3.3:49509515-49509534 MS.gene08082:CDS 45.0%
! GGTTGGCTGACATATCCATA+TGG + chr3.3:49509362-49509381 None:intergenic 45.0%
AAAGATTCGGCTGAGCACAC+AGG - chr3.3:49509719-49509738 MS.gene08082:CDS 50.0%
AATTCAGGGGGAAGACCATC+TGG - chr3.3:49509845-49509864 MS.gene08082:CDS 50.0%
ATTCAGGGGGAAGACCATCT+GGG - chr3.3:49509846-49509865 MS.gene08082:CDS 50.0%
CGAACAACAGGAAATGGTGG+AGG + chr3.3:49509389-49509408 None:intergenic 50.0%
CGCAGTCGAACAACAGGAAA+TGG + chr3.3:49509395-49509414 None:intergenic 50.0%
GAGCTATTCCCTTCAACGCA+TGG - chr3.3:49509953-49509972 MS.gene08082:intron 50.0%
TTTCCTGTTGTTCGACTGCG+CGG - chr3.3:49509395-49509414 MS.gene08082:CDS 50.0%
! ACAACAGGAAATGGTGGAGG+AGG + chr3.3:49509386-49509405 None:intergenic 50.0%
! CAAGGAGTATGTGTTGTCGG+AGG - chr3.3:49509802-49509821 MS.gene08082:CDS 50.0%
! CGGTGAAGGCTTCTGTGTTT+TGG - chr3.3:49509514-49509533 MS.gene08082:CDS 50.0%
ACCATCTGGGGAGGCTTATG+TGG - chr3.3:49509859-49509878 MS.gene08082:CDS 55.0%
CCCTTCAACGCATGGTGAGA+CGG - chr3.3:49509961-49509980 MS.gene08082:intron 55.0%
CCGTCTCACCATGCGTTGAA+GGG + chr3.3:49509964-49509983 None:intergenic 55.0%
GAGGTTTCAGATGCTCGAGG+CGG - chr3.3:49509650-49509669 MS.gene08082:CDS 55.0%
GATGTGGCCGAGTTCTTCCA+TGG - chr3.3:49509443-49509462 MS.gene08082:CDS 55.0%
TCCACATAAGCCTCCCCAGA+TGG + chr3.3:49509863-49509882 None:intergenic 55.0%
TCCGTCTCACCATGCGTTGA+AGG + chr3.3:49509965-49509984 None:intergenic 55.0%
TTCAGGGGGAAGACCATCTG+GGG - chr3.3:49509847-49509866 MS.gene08082:CDS 55.0%
! ACAGGAAATGGTGGAGGAGG+AGG + chr3.3:49509383-49509402 None:intergenic 55.0%
! GAAATGGTGGAGGAGGAGGT+TGG + chr3.3:49509379-49509398 None:intergenic 55.0%
! GGTGGCAAGTTTTCCGGTGA+AGG - chr3.3:49509500-49509519 MS.gene08082:CDS 55.0%
!! ACACAGGAGTGTTGCGACTG+AGG - chr3.3:49509735-49509754 MS.gene08082:CDS 55.0%
!! ACAGGAGTGTTGCGACTGAG+GGG - chr3.3:49509737-49509756 MS.gene08082:CDS 55.0%
!! CACAGGAGTGTTGCGACTGA+GGG - chr3.3:49509736-49509755 MS.gene08082:CDS 55.0%
!! TGGTGGTGATGGGCGTGAAA+TGG - chr3.3:49509256-49509275 MS.gene08082:CDS 55.0%
ACGTCAGCGGACAGTTGACG+CGG - chr3.3:49509286-49509305 MS.gene08082:CDS 60.0%
AGACCGCGCAGTCGAACAAC+AGG + chr3.3:49509401-49509420 None:intergenic 60.0%
!! CTCGGGGAGCACTATGACGA+GGG + chr3.3:49509679-49509698 None:intergenic 60.0%
!! TCGGGGAGCACTATGACGAG+GGG + chr3.3:49509678-49509697 None:intergenic 60.0%
AGGGGGAAGACCATCTGGGG+AGG - chr3.3:49509850-49509869 MS.gene08082:CDS 65.0%
CGTCAGCGGACAGTTGACGC+GGG - chr3.3:49509287-49509306 MS.gene08082:CDS 65.0%
!! GCTCGGGGAGCACTATGACG+AGG + chr3.3:49509680-49509699 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 49509135 49510009 49509135 ID=MS.gene08082
chr3.3 mRNA 49509135 49510009 49509135 ID=MS.gene08082.t1;Parent=MS.gene08082
chr3.3 exon 49509978 49510009 49509978 ID=MS.gene08082.t1.exon1;Parent=MS.gene08082.t1
chr3.3 CDS 49509978 49510009 49509978 ID=cds.MS.gene08082.t1;Parent=MS.gene08082.t1
chr3.3 exon 49509135 49509900 49509135 ID=MS.gene08082.t1.exon2;Parent=MS.gene08082.t1
chr3.3 CDS 49509135 49509900 49509135 ID=cds.MS.gene08082.t1;Parent=MS.gene08082.t1
Gene Sequence

>MS.gene08082

ATGATTACTGAAACTTTAGAAGACAACCTAGAGTACTTAAACTTATGTCTTTGTTGACTATAATGATAAGCTTATTGACTTCAATTTTGGTTATTGATGAAAATTTCAGTAAATTTGCTGATGGTGGTGATGGGCGTGAAATGGCTTCAAAACGTCAGCGGACAGTTGACGCGGGATCTTCATTCTATGATTCCCCTGGTTCCAGTTATATGTACAATCCATCTCCATATGGATATGTCAGCCAACCTCCTCCTCCACCATTTCCTGTTGTTCGACTGCGCGGTCTTCCCTTTGATTGCACTGAAACTGATGTGGCCGAGTTCTTCCATGGTCTAGACATAGTTGATGTTCTTTTTGTTCACAAAGGTGGCAAGTTTTCCGGTGAAGGCTTCTGTGTTTTGGGATATCCTCTTCAAGTTGACTTTGCTCTTCAAAGAAACAGGCAGAACATAGGTAGAAGATATGTTGAAGTTTTCAGAAGTAAGAGGCAGGAATACTACAAGGCAGTTGCAAATGAGGTTTCAGATGCTCGAGGCGGTTCCCCTCGTCATAGTGCTCCCCGAGCTAAATCATATGATGAAGGAAAAGATTCGGCTGAGCACACAGGAGTGTTGCGACTGAGGGGATTACCATTTTCTGCTAATAAGGATGACATAATGGAGTTCTTCAAGGAGTATGTGTTGTCGGAGGATTCGATCCACATTGTAATGAATTCAGGGGGAAGACCATCTGGGGAGGCTTATGTGGAATTTGAAAATGCTGATGATTCAAAAGCAGCCATGGCAAAGGATAGAATGACGCTTGGGAGTCGCTATATAGAGCTATTCCCTTCAACGCATGGTGAGACGGAAGATGCAATTTCAAGAGGACGGTGA

Protein sequence

>MS.gene08082.t1

MITETLEDNLDKFADGGDGREMASKRQRTVDAGSSFYDSPGSSYMYNPSPYGYVSQPPPPPFPVVRLRGLPFDCTETDVAEFFHGLDIVDVLFVHKGGKFSGEGFCVLGYPLQVDFALQRNRQNIGRRYVEVFRSKRQEYYKAVANEVSDARGGSPRHSAPRAKSYDEGKDSAEHTGVLRLRGLPFSANKDDIMEFFKEYVLSEDSIHIVMNSGGRPSGEAYVEFENADDSKAAMAKDRMTLGSRYIELFPSTHGETEDAISRGR