AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08698

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08698.t1 MTR_4g058860 98.555 346 4 1 1 345 4 349 0.0 681
MS.gene08698.t1 MTR_2g094640 83.478 345 54 3 1 343 4 347 0.0 571
MS.gene08698.t1 MTR_3g094260 65.116 344 116 2 2 342 5 347 1.95e-150 427
MS.gene08698.t1 MTR_3g094270 66.462 325 108 1 2 326 5 328 2.38e-144 412
MS.gene08698.t1 MTR_1g026590 37.267 322 192 2 10 321 14 335 7.52e-58 191
MS.gene08698.t1 MTR_8g092190 34.848 330 206 6 9 332 15 341 1.54e-56 187
MS.gene08698.t1 MTR_1291s0010 81.481 108 20 0 1 108 4 111 2.63e-55 176
MS.gene08698.t1 MTR_3g095130 36.333 300 189 2 3 301 10 308 4.24e-55 183
MS.gene08698.t1 MTR_5g004620 39.203 301 178 3 3 299 9 308 2.65e-54 182
MS.gene08698.t1 MTR_1g098240 36.860 293 184 1 10 301 16 308 7.25e-53 177
MS.gene08698.t1 MTR_3g095130 36.797 231 145 1 72 301 29 259 1.34e-44 154
MS.gene08698.t1 MTR_2g038410 30.375 293 196 2 19 307 66 354 4.56e-35 132
MS.gene08698.t1 MTR_5g096310 29.530 298 202 2 7 300 55 348 8.12e-32 123
MS.gene08698.t1 MTR_3g072380 25.478 314 223 5 19 325 26 335 2.65e-22 96.3
MS.gene08698.t1 MTR_1g030320 24.611 321 232 5 17 329 23 341 1.44e-21 94.4
MS.gene08698.t1 MTR_0011s0320 26.934 349 209 16 19 344 25 350 2.19e-20 90.9
MS.gene08698.t1 MTR_4g114360 26.667 345 209 15 19 343 25 345 6.95e-18 84.0
MS.gene08698.t1 MTR_7g110380 26.050 357 230 11 1 344 8 343 5.06e-17 81.6
MS.gene08698.t1 MTR_3g107470 24.699 332 218 12 19 330 29 348 8.15e-17 80.9
MS.gene08698.t1 MTR_4g123900 26.062 353 226 13 1 331 4 343 5.22e-12 66.6
MS.gene08698.t1 MTR_0662s0010 28.358 268 157 14 19 266 25 277 1.48e-11 64.7
MS.gene08698.t1 MTR_3g070000 26.875 320 196 11 2 301 19 320 2.00e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08698.t1 AT2G28315 75.942 345 77 3 1 345 4 342 0.0 515
MS.gene08698.t1 AT2G28315 82.392 301 53 0 1 301 4 304 0.0 505
MS.gene08698.t1 AT1G06890 65.143 350 111 2 3 342 6 354 5.30e-151 429
MS.gene08698.t1 AT1G06890 65.143 350 111 2 3 342 6 354 5.30e-151 429
MS.gene08698.t1 AT1G06890 68.827 324 100 1 3 326 6 328 1.73e-150 427
MS.gene08698.t1 AT1G06890 68.827 324 100 1 3 326 6 328 2.15e-150 427
MS.gene08698.t1 AT2G30460 65.805 348 108 4 3 342 6 350 1.42e-148 423
MS.gene08698.t1 AT2G30460 65.805 348 108 4 3 342 6 350 1.42e-148 423
MS.gene08698.t1 AT2G30460 64.744 312 101 4 18 326 2 307 1.27e-137 394
MS.gene08698.t1 AT2G30460 64.744 312 101 4 18 326 2 307 1.27e-137 394
MS.gene08698.t1 AT2G30460 64.744 312 101 4 18 326 2 307 1.27e-137 394
MS.gene08698.t1 AT2G30460 71.101 218 63 0 3 220 6 223 1.02e-97 293
MS.gene08698.t1 AT5G42420 37.278 338 205 3 2 337 9 341 1.88e-62 203
MS.gene08698.t1 AT1G21070 37.179 312 194 1 9 318 16 327 1.04e-60 198
MS.gene08698.t1 AT4G09810 37.255 306 190 2 10 313 16 321 7.88e-60 196
MS.gene08698.t1 AT4G39390 33.841 328 204 2 9 335 16 331 4.32e-59 194
MS.gene08698.t1 AT4G39390 33.841 328 204 2 9 335 20 335 4.33e-59 194
MS.gene08698.t1 AT4G39390 33.841 328 204 2 9 335 20 335 4.33e-59 194
MS.gene08698.t1 AT1G76670 35.849 318 202 1 3 318 9 326 4.15e-58 191
MS.gene08698.t1 AT1G76670 35.849 318 202 1 3 318 9 326 4.15e-58 191
MS.gene08698.t1 AT1G34020 36.218 312 192 3 15 319 21 332 1.05e-57 190
MS.gene08698.t1 AT1G34020 36.218 312 192 3 15 319 21 332 1.05e-57 190
MS.gene08698.t1 AT1G34020 36.218 312 192 3 15 319 21 332 1.05e-57 190
MS.gene08698.t1 AT4G09810 39.367 221 133 1 82 301 31 251 4.39e-49 166
MS.gene08698.t1 AT4G09810 37.681 207 129 0 10 216 16 222 9.49e-40 140
MS.gene08698.t1 AT5G57100 31.034 290 192 2 11 296 67 352 5.90e-38 140
MS.gene08698.t1 AT5G55950 29.856 278 187 2 19 292 68 341 8.61e-34 129
MS.gene08698.t1 AT5G55950 29.856 278 187 2 19 292 8 281 1.06e-33 127
MS.gene08698.t1 AT5G42420 29.904 311 148 5 2 310 9 251 1.23e-31 120
MS.gene08698.t1 AT5G55950 29.588 267 180 2 30 292 1 263 1.23e-30 119
MS.gene08698.t1 AT5G57100 30.000 240 160 2 7 242 63 298 1.62e-27 110
MS.gene08698.t1 AT5G57100 32.308 195 128 1 7 197 63 257 3.77e-25 102
MS.gene08698.t1 AT5G57100 30.198 202 137 1 95 296 40 237 2.57e-23 97.8
MS.gene08698.t1 AT5G55950 28.333 240 164 2 7 242 56 291 2.99e-23 98.6
MS.gene08698.t1 AT4G32390 26.113 337 217 11 19 341 29 347 9.22e-20 89.4
MS.gene08698.t1 AT5G25400 26.409 337 215 13 19 341 29 346 1.75e-19 88.6
MS.gene08698.t1 AT1G21870 25.979 281 199 4 19 292 26 304 2.71e-19 87.8
MS.gene08698.t1 AT1G77610 25.267 281 201 4 19 292 20 298 2.16e-18 85.1
MS.gene08698.t1 AT2G25520 25.740 338 218 11 19 342 29 347 2.39e-18 85.1
MS.gene08698.t1 AT5G11230 25.959 339 216 12 19 341 29 348 8.15e-18 84.0
MS.gene08698.t1 AT4G32272 26.115 314 202 9 2 293 34 339 1.24e-14 74.3
MS.gene08698.t1 AT4G32272 26.073 303 195 8 13 293 59 354 4.64e-14 72.8
MS.gene08698.t1 AT5G05820 26.333 300 193 8 17 301 20 306 3.53e-11 63.9
MS.gene08698.t1 AT3G17430 25.000 352 224 14 19 340 23 364 4.72e-11 63.9
MS.gene08698.t1 AT3G17430 25.000 352 224 14 19 340 23 364 4.72e-11 63.9
MS.gene08698.t1 AT3G11320 26.073 303 190 9 17 301 20 306 6.95e-11 62.8

Find 57 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTGAAAGGAACATTAT+TGG 0.175320 4.2:-44612750 MS.gene08698:CDS
GCAGCAATTCTCTTTGTTTC+AGG 0.186376 4.2:-44613364 MS.gene08698:CDS
TCTCTATTCTTCTTACTTGT+TGG 0.215453 4.2:-44613844 MS.gene08698:CDS
CTCAAGACTTGTCTTGTTCT+AGG 0.234530 4.2:-44612801 MS.gene08698:CDS
ACTTGTCTTGTTCTAGGATT+TGG 0.249840 4.2:-44612795 MS.gene08698:CDS
TTTAGCACCTTTCTAGTAAT+AGG 0.278992 4.2:-44612861 MS.gene08698:CDS
CAATTCCATTGGATTCTATC+AGG 0.280510 4.2:-44614528 MS.gene08698:intron
AAAACCAAGATTGCTCATTA+AGG 0.290101 4.2:+44614819 None:intergenic
GTTCTCTTGAGTGCAGAAAT+AGG 0.307448 4.2:+44612692 None:intergenic
GTTACCTGAACTGCTTCTTT+AGG 0.308343 4.2:+44613983 None:intergenic
ACTTACAAGTTGGCATTTGA+TGG 0.352879 4.2:-44614684 MS.gene08698:CDS
ATAGAGAGAGTTTAATATTC+CGG 0.359278 4.2:+44613860 None:intergenic
AAAGCCTTAATGAGCAATCT+TGG 0.364932 4.2:-44614823 MS.gene08698:CDS
CCCAACACTTGATATGTTAC+TGG 0.372610 4.2:+44612828 None:intergenic
TTCTTCTTACTTGTTGGAGT+TGG 0.376842 4.2:-44613838 MS.gene08698:CDS
AGCCTTGGCTTCAATTCCAT+TGG 0.380768 4.2:-44614539 MS.gene08698:CDS
CGATCCTCCTTTGTCGTCTC+AGG 0.401787 4.2:-44612652 MS.gene08698:intron
TTCCAACAAAATTGAGTTGA+AGG 0.414250 4.2:+44613797 None:intergenic
ATGACAGTCTTCATGTCAAC+AGG 0.417274 4.2:+44614608 None:intergenic
AGTCTTCATGTCAACAGGTT+TGG 0.432306 4.2:+44614613 None:intergenic
GGATTTCTCAACTTGAGCCT+TGG 0.433359 4.2:-44614554 MS.gene08698:CDS
CTTTGTTTCAGGCCCTCTTG+TGG 0.448438 4.2:-44613353 MS.gene08698:CDS
TCTACGTTTCAATCTGTTTG+AGG 0.451737 4.2:-44614636 MS.gene08698:CDS
CAAATATTCTCCTCTTGTCT+TGG 0.453651 4.2:-44613296 MS.gene08698:intron
TAATGTTCCTTTCAGTGAAA+GGG 0.465034 4.2:+44612754 None:intergenic
ATTCTAAATGGTGTCTCCAT+TGG 0.473165 4.2:-44614575 MS.gene08698:CDS
TTTCAGGCCCTCTTGTGGAT+CGG 0.483362 4.2:-44613348 MS.gene08698:CDS
CCAGTAACATATCAAGTGTT+GGG 0.487472 4.2:-44612828 MS.gene08698:CDS
ATAATGTTCCTTTCAGTGAA+AGG 0.490738 4.2:+44612753 None:intergenic
AAGAGAATTGCTGCTTGGAA+TGG 0.498395 4.2:+44613373 None:intergenic
GATAGCTAGAAGTGAAAGAA+TGG 0.517732 4.2:+44613774 None:intergenic
TCCAGTAACATATCAAGTGT+TGG 0.519813 4.2:-44612829 MS.gene08698:CDS
TGGTGCACTATTCCTTTCTG+TGG 0.529063 4.2:-44614875 MS.gene08698:CDS
TCCTTTCAGTGAAAGGGTCA+TGG 0.532382 4.2:+44612760 None:intergenic
TCCATGACCCTTTCACTGAA+AGG 0.541368 4.2:-44612761 MS.gene08698:CDS
AAACAAAGAGAATTGCTGCT+TGG 0.548746 4.2:+44613368 None:intergenic
GTTTCTAGCATAACTGTGAA+TGG 0.548850 4.2:+44614012 None:intergenic
GGCTTTGTTGCATATGACAA+TGG 0.551399 4.2:+44614840 None:intergenic
ACATTACTTACCAAGACAAG+AGG 0.560103 4.2:+44613286 None:intergenic
GCTATCATAACAACATGTGT+TGG 0.567567 4.2:-44613757 MS.gene08698:CDS
CAGCCACAACACTTACAAGT+TGG 0.572331 4.2:-44614694 MS.gene08698:CDS
ATCCAATGGAATTGAAGCCA+AGG 0.586597 4.2:+44614537 None:intergenic
TGAAACGTAGAGCCACATGA+AGG 0.588235 4.2:+44614648 None:intergenic
AGACAGAAGAAGCCACAGAA+AGG 0.589376 4.2:+44614863 None:intergenic
GCTCAAGTTGAGAAATCCAA+TGG 0.598682 4.2:+44614559 None:intergenic
CTGCTTGGAATGGAGCAGAC+TGG 0.603506 4.2:+44613383 None:intergenic
GTAAACCTGATAGAATCCAA+TGG 0.616739 4.2:+44614523 None:intergenic
CTAGAACAAGACAAGTCTTG+AGG 0.626745 4.2:+44612802 None:intergenic
AAAATTACCTGAGACGACAA+AGG 0.636716 4.2:+44612645 None:intergenic
GTTAGCATCCGATCCACAAG+AGG 0.641060 4.2:+44613340 None:intergenic
GAACAAGACAAGTCTTGAGG+TGG 0.660929 4.2:+44612805 None:intergenic
GAAACGTAGAGCCACATGAA+GGG 0.662091 4.2:+44614649 None:intergenic
ATGCCAACTTGTAAGTGTTG+TGG 0.665045 4.2:+44614691 None:intergenic
ATTACCTGAGACGACAAAGG+AGG 0.680324 4.2:+44612648 None:intergenic
TGAGTCTAAGAAATCAAACA+AGG 0.682301 4.2:-44612482 MS.gene08698:CDS
TTAGCATCCGATCCACAAGA+GGG 0.706454 4.2:+44613341 None:intergenic
AGACTGGTAGAGAAGTTGTG+TGG 0.708746 4.2:+44613399 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATATATTTATTACTAG+TGG + chr4.2:44612887-44612906 None:intergenic 10.0%
!! TGTTAAAAAAAAATATGTAT+CGG + chr4.2:44614230-44614249 None:intergenic 10.0%
!! ATACATAAACACTTAATTTA+GGG - chr4.2:44613862-44613881 MS.gene08698:CDS 15.0%
!! CATTACATTAGTATTAATTA+GGG + chr4.2:44614123-44614142 None:intergenic 15.0%
!! GTTAAAAAAAAATATGTATC+GGG + chr4.2:44614229-44614248 None:intergenic 15.0%
!! TATACATAAACACTTAATTT+AGG - chr4.2:44613861-44613880 MS.gene08698:CDS 15.0%
!!! TATCTTTGATTATATTTTTC+AGG - chr4.2:44614797-44614816 MS.gene08698:intron 15.0%
!!! TTATTTTTGTCACATTAATT+TGG - chr4.2:44612650-44612669 MS.gene08698:intron 15.0%
!!! TTGAATTTAACTTTATGTAT+TGG + chr4.2:44614258-44614277 None:intergenic 15.0%
!! AATATTAACATGCTAACAAT+AGG + chr4.2:44613667-44613686 None:intergenic 20.0%
!! CCATTACATTAGTATTAATT+AGG + chr4.2:44614124-44614143 None:intergenic 20.0%
!! CCTAATTAATACTAATGTAA+TGG - chr4.2:44614121-44614140 MS.gene08698:intron 20.0%
!!! AATTTAACTTTATGTATTGG+TGG + chr4.2:44614255-44614274 None:intergenic 20.0%
!!! GTACTTTTCATGATAAAAAA+TGG - chr4.2:44612614-44612633 MS.gene08698:intron 20.0%
! AATATTGATACATGCATCAA+AGG + chr4.2:44612940-44612959 None:intergenic 25.0%
! ATAGAGAGAGTTTAATATTC+CGG + chr4.2:44613512-44613531 None:intergenic 25.0%
! CAAAAATGATAATTACCGAA+TGG + chr4.2:44612576-44612595 None:intergenic 25.0%
! GAACATTAAAGGATACAATT+TGG + chr4.2:44613638-44613657 None:intergenic 25.0%
!! ACTTTTGAATGGTAAAAACA+TGG - chr4.2:44614845-44614864 MS.gene08698:CDS 25.0%
!! TGTTTTTCCTATTACTAGAA+AGG + chr4.2:44614518-44614537 None:intergenic 25.0%
!!! ATAAATTCACCACTTTTGAA+TGG - chr4.2:44614834-44614853 MS.gene08698:CDS 25.0%
!!! ATGTTTTTACCATTCAAAAG+TGG + chr4.2:44614846-44614865 None:intergenic 25.0%
!!! CACTTATTTTCAATATCACT+AGG + chr4.2:44613429-44613448 None:intergenic 25.0%
!!! TAATGTAATGGATAACTAGA+AGG - chr4.2:44614133-44614152 MS.gene08698:intron 25.0%
AAAACCAAGATTGCTCATTA+AGG + chr4.2:44612553-44612572 None:intergenic 30.0%
ATAATGTTCCTTTCAGTGAA+AGG + chr4.2:44614619-44614638 None:intergenic 30.0%
ATAGGAAGACAGAACATTAA+AGG + chr4.2:44613649-44613668 None:intergenic 30.0%
ATGCATACCAATGTTTCAAT+CGG + chr4.2:44613261-44613280 None:intergenic 30.0%
ATTGAAAATAAGTGTGACCA+TGG - chr4.2:44613435-44613454 MS.gene08698:CDS 30.0%
CAAATAAAATCTTGACCCAA+AGG - chr4.2:44613213-44613232 MS.gene08698:intron 30.0%
GAGAAACTATGTGAGAAAAA+TGG + chr4.2:44614754-44614773 None:intergenic 30.0%
TAAAAACATGGATGAGGAAA+AGG - chr4.2:44614857-44614876 MS.gene08698:CDS 30.0%
TAATGTTCCTTTCAGTGAAA+GGG + chr4.2:44614618-44614637 None:intergenic 30.0%
TCTCTATTCTTCTTACTTGT+TGG - chr4.2:44613525-44613544 MS.gene08698:intron 30.0%
TGAGTCTAAGAAATCAAACA+AGG - chr4.2:44614887-44614906 MS.gene08698:CDS 30.0%
TTAGGGCTTGAAAGTTAAAA+AGG - chr4.2:44613879-44613898 MS.gene08698:intron 30.0%
TTCACTGAAAGGAACATTAT+TGG - chr4.2:44614619-44614638 MS.gene08698:CDS 30.0%
! GATGAAAGTCTGAAATACAA+AGG + chr4.2:44614455-44614474 None:intergenic 30.0%
! TTTAGCACCTTTCTAGTAAT+AGG - chr4.2:44614508-44614527 MS.gene08698:intron 30.0%
! TTTGATGATTTAACTACTCG+TGG - chr4.2:44613686-44613705 MS.gene08698:intron 30.0%
!! ATGCTTTTTGGCATTCTAAA+TGG - chr4.2:44612782-44612801 MS.gene08698:CDS 30.0%
!! TTCCAACAAAATTGAGTTGA+AGG + chr4.2:44613575-44613594 None:intergenic 30.0%
AAAACTCAATTGGGGGTTAT+TGG - chr4.2:44612474-44612493 MS.gene08698:CDS 35.0%
AAAATTACCTGAGACGACAA+AGG + chr4.2:44614727-44614746 None:intergenic 35.0%
AAACAAAGAGAATTGCTGCT+TGG + chr4.2:44614004-44614023 None:intergenic 35.0%
AAAGCCTTAATGAGCAATCT+TGG - chr4.2:44612546-44612565 MS.gene08698:CDS 35.0%
AAGCAGGCTGCAAAATAATT+GGG + chr4.2:44613035-44613054 None:intergenic 35.0%
ACATTACTTACCAAGACAAG+AGG + chr4.2:44614086-44614105 None:intergenic 35.0%
ATCGAATTGGTGAAACCTTT+GGG + chr4.2:44613231-44613250 None:intergenic 35.0%
ATTCTAAATGGTGTCTCCAT+TGG - chr4.2:44612794-44612813 MS.gene08698:CDS 35.0%
ATTGGCTAAATTGGATGGTT+CGG + chr4.2:44612975-44612994 None:intergenic 35.0%
CAAATATTCTCCTCTTGTCT+TGG - chr4.2:44614073-44614092 MS.gene08698:intron 35.0%
CAATTCCATTGGATTCTATC+AGG - chr4.2:44612841-44612860 MS.gene08698:CDS 35.0%
CCAGTAACATATCAAGTGTT+GGG - chr4.2:44614541-44614560 MS.gene08698:CDS 35.0%
GAATGGTAAAAACATGGATG+AGG - chr4.2:44614851-44614870 MS.gene08698:CDS 35.0%
GATAGCTAGAAGTGAAAGAA+TGG + chr4.2:44613598-44613617 None:intergenic 35.0%
GCTATCATAACAACATGTGT+TGG - chr4.2:44613612-44613631 MS.gene08698:intron 35.0%
GGATTGAATTGGGGTTAAAT+CGG - chr4.2:44613060-44613079 MS.gene08698:intron 35.0%
GTAAACCTGATAGAATCCAA+TGG + chr4.2:44612849-44612868 None:intergenic 35.0%
GTTCAATTGGCTAAATTGGA+TGG + chr4.2:44612980-44612999 None:intergenic 35.0%
TAAGCAATTTAGCATGACCA+TGG + chr4.2:44613455-44613474 None:intergenic 35.0%
TCCAGTAACATATCAAGTGT+TGG - chr4.2:44614540-44614559 MS.gene08698:CDS 35.0%
TCTACGTTTCAATCTGTTTG+AGG - chr4.2:44612733-44612752 MS.gene08698:CDS 35.0%
TTGTTGATCATCCTGTTCAA+CGG - chr4.2:44613173-44613192 MS.gene08698:intron 35.0%
! ACTTACAAGTTGGCATTTGA+TGG - chr4.2:44612685-44612704 MS.gene08698:CDS 35.0%
! ATGAAGACTGTCATGCTTTT+TGG - chr4.2:44612770-44612789 MS.gene08698:CDS 35.0%
! ATTTTACTACTATGTGCAGC+CGG - chr4.2:44613490-44613509 MS.gene08698:intron 35.0%
! GACCTTCAACTCAATTTTGT+TGG - chr4.2:44613570-44613589 MS.gene08698:intron 35.0%
! GGAATACTAGTAGCAGTTTT+TGG - chr4.2:44614640-44614659 MS.gene08698:CDS 35.0%
! GTATGCAAAGACATTTTGCT+TGG + chr4.2:44614054-44614073 None:intergenic 35.0%
! TTCTTCTTACTTGTTGGAGT+TGG - chr4.2:44613531-44613550 MS.gene08698:intron 35.0%
! TTTTCCTAAAGAAGCAGTTC+AGG - chr4.2:44613382-44613401 MS.gene08698:CDS 35.0%
!! ACTAGTAGCAGTTTTTGGAA+TGG - chr4.2:44614645-44614664 MS.gene08698:CDS 35.0%
!! ACTTGTCTTGTTCTAGGATT+TGG - chr4.2:44614574-44614593 MS.gene08698:CDS 35.0%
!! AGCAATCTTGGTTTTCCATT+CGG - chr4.2:44612558-44612577 MS.gene08698:intron 35.0%
!! GTTTCTAGCATAACTGTGAA+TGG + chr4.2:44613360-44613379 None:intergenic 35.0%
!! TGCTTGAAACGGATTGAATT+GGG - chr4.2:44613050-44613069 MS.gene08698:intron 35.0%
!!! CTAGTAGCAGTTTTTGGAAT+GGG - chr4.2:44614646-44614665 MS.gene08698:CDS 35.0%
AAACTGTTTGCGAGAGCTTA+TGG - chr4.2:44613749-44613768 MS.gene08698:intron 40.0%
AAGTTATCCGAGAGAGCTTA+TGG + chr4.2:44613793-44613812 None:intergenic 40.0%
AGTCTTCATGTCAACAGGTT+TGG + chr4.2:44612759-44612778 None:intergenic 40.0%
ATCCAATGGAATTGAAGCCA+AGG + chr4.2:44612835-44612854 None:intergenic 40.0%
ATGACAGTCTTCATGTCAAC+AGG + chr4.2:44612764-44612783 None:intergenic 40.0%
CAAGCAGGCTGCAAAATAAT+TGG + chr4.2:44613036-44613055 None:intergenic 40.0%
CAATTCAATCCGTTTCAAGC+AGG + chr4.2:44613051-44613070 None:intergenic 40.0%
CCCAACACTTGATATGTTAC+TGG + chr4.2:44614544-44614563 None:intergenic 40.0%
CTAGAACAAGACAAGTCTTG+AGG + chr4.2:44614570-44614589 None:intergenic 40.0%
CTCGGTTCAATTGGCTAAAT+TGG + chr4.2:44612984-44613003 None:intergenic 40.0%
GAATTGGGGTTAAATCGGTT+TGG - chr4.2:44613065-44613084 MS.gene08698:intron 40.0%
GATCGAATTGGTGAAACCTT+TGG + chr4.2:44613232-44613251 None:intergenic 40.0%
GGTTTCACCAATTCGATCTT+CGG - chr4.2:44613234-44613253 MS.gene08698:intron 40.0%
GTTACCTGAACTGCTTCTTT+AGG + chr4.2:44613389-44613408 None:intergenic 40.0%
GTTCTCTTGAGTGCAGAAAT+AGG + chr4.2:44614680-44614699 None:intergenic 40.0%
TAGCCAATTGAACCGAGAAT+CGG - chr4.2:44612987-44613006 MS.gene08698:intron 40.0%
! AAGAGAATTGCTGCTTGGAA+TGG + chr4.2:44613999-44614018 None:intergenic 40.0%
! ATGCCAACTTGTAAGTGTTG+TGG + chr4.2:44612681-44612700 None:intergenic 40.0%
! ATTTTGCAGCCTGCTTGAAA+CGG - chr4.2:44613039-44613058 MS.gene08698:intron 40.0%
! CTCAAGACTTGTCTTGTTCT+AGG - chr4.2:44614568-44614587 MS.gene08698:CDS 40.0%
! GCAGCAATTCTCTTTGTTTC+AGG - chr4.2:44614005-44614024 MS.gene08698:CDS 40.0%
! TAACCGATTCTCGGTTCAAT+TGG + chr4.2:44612993-44613012 None:intergenic 40.0%
!! CTGCTTGAAACGGATTGAAT+TGG - chr4.2:44613049-44613068 MS.gene08698:intron 40.0%
!! GCTCAAGTTGAGAAATCCAA+TGG + chr4.2:44612813-44612832 None:intergenic 40.0%
!! GCTTGAAACGGATTGAATTG+GGG - chr4.2:44613051-44613070 MS.gene08698:intron 40.0%
!! GGCTTTGTTGCATATGACAA+TGG + chr4.2:44612532-44612551 None:intergenic 40.0%
!! TACTTTTTGCACCCTTCATG+TGG - chr4.2:44612709-44612728 MS.gene08698:CDS 40.0%
AATCGGACCGAAGATCGAAT+TGG + chr4.2:44613244-44613263 None:intergenic 45.0%
AGACAGAAGAAGCCACAGAA+AGG + chr4.2:44612509-44612528 None:intergenic 45.0%
AGACTGGTAGAGAAGTTGTG+TGG + chr4.2:44613973-44613992 None:intergenic 45.0%
AGCCTTGGCTTCAATTCCAT+TGG - chr4.2:44612830-44612849 MS.gene08698:CDS 45.0%
ATTACCTGAGACGACAAAGG+AGG + chr4.2:44614724-44614743 None:intergenic 45.0%
CAGCCACAACACTTACAAGT+TGG - chr4.2:44612675-44612694 MS.gene08698:CDS 45.0%
CAGTTTAACCACCGTTGAAC+AGG + chr4.2:44613187-44613206 None:intergenic 45.0%
CTTCGGTCCGATTGAAACAT+TGG - chr4.2:44613251-44613270 MS.gene08698:intron 45.0%
GAAACGTAGAGCCACATGAA+GGG + chr4.2:44612723-44612742 None:intergenic 45.0%
GAACAAGACAAGTCTTGAGG+TGG + chr4.2:44614567-44614586 None:intergenic 45.0%
GACAAGTCCATAAGCTCTCT+CGG - chr4.2:44613783-44613802 MS.gene08698:CDS 45.0%
GGATTTCTCAACTTGAGCCT+TGG - chr4.2:44612815-44612834 MS.gene08698:CDS 45.0%
TCCATGACCCTTTCACTGAA+AGG - chr4.2:44614608-44614627 MS.gene08698:CDS 45.0%
TCCTTTCAGTGAAAGGGTCA+TGG + chr4.2:44614612-44614631 None:intergenic 45.0%
TGAAACGTAGAGCCACATGA+AGG + chr4.2:44612724-44612743 None:intergenic 45.0%
TGAACCGAGAATCGGTTAGT+TGG - chr4.2:44612995-44613014 MS.gene08698:intron 45.0%
TGGTGCACTATTCCTTTCTG+TGG - chr4.2:44612494-44612513 MS.gene08698:CDS 45.0%
TTAGCATCCGATCCACAAGA+GGG + chr4.2:44614031-44614050 None:intergenic 45.0%
TTGATCATCCTGTTCAACGG+TGG - chr4.2:44613176-44613195 MS.gene08698:intron 45.0%
TTGGTTGCGGTAGATTCAAC+TGG - chr4.2:44613084-44613103 MS.gene08698:intron 45.0%
! CAACTTGTAAGTGTTGTGGC+TGG + chr4.2:44612677-44612696 None:intergenic 45.0%
! CTGACCAACTAACCGATTCT+CGG + chr4.2:44613002-44613021 None:intergenic 45.0%
! GAATCGGTTAGTTGGTCAGT+TGG - chr4.2:44613003-44613022 MS.gene08698:intron 45.0%
! GTCGTGTTAGTTTCAGACAC+TGG + chr4.2:44614207-44614226 None:intergenic 45.0%
!! GGGTTAAATCGGTTTGGTTG+CGG - chr4.2:44613071-44613090 MS.gene08698:intron 45.0%
!! AAAAAAATGTTAAAAATATT+TGG - chr4.2:44612913-44612932 MS.gene08698:intron 5.0%
!! AGTAATAAATATATTAATTA+TGG - chr4.2:44612889-44612908 MS.gene08698:CDS 5.0%
!!! TAAAAAGATATATTTAAAAT+AGG + chr4.2:44613146-44613165 None:intergenic 5.0%
GTTAGCATCCGATCCACAAG+AGG + chr4.2:44614032-44614051 None:intergenic 50.0%
TTTCAGGCCCTCTTGTGGAT+CGG - chr4.2:44614021-44614040 MS.gene08698:CDS 50.0%
! CTTTGTTTCAGGCCCTCTTG+TGG - chr4.2:44614016-44614035 MS.gene08698:CDS 50.0%
CGATCCTCCTTTGTCGTCTC+AGG - chr4.2:44614717-44614736 MS.gene08698:intron 55.0%
CTGCTTGGAATGGAGCAGAC+TGG + chr4.2:44613989-44614008 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 44612468 44614923 44612468 ID=MS.gene08698
chr4.2 mRNA 44612468 44614923 44612468 ID=MS.gene08698.t1;Parent=MS.gene08698
chr4.2 exon 44614812 44614923 44614812 ID=MS.gene08698.t1.exon1;Parent=MS.gene08698.t1
chr4.2 CDS 44614812 44614923 44614812 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44614529 44614713 44614529 ID=MS.gene08698.t1.exon2;Parent=MS.gene08698.t1
chr4.2 CDS 44614529 44614713 44614529 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44613988 44614055 44613988 ID=MS.gene08698.t1.exon3;Parent=MS.gene08698.t1
chr4.2 CDS 44613988 44614055 44613988 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44613750 44613882 44613750 ID=MS.gene08698.t1.exon4;Parent=MS.gene08698.t1
chr4.2 CDS 44613750 44613882 44613750 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44613297 44613458 44613297 ID=MS.gene08698.t1.exon5;Parent=MS.gene08698.t1
chr4.2 CDS 44613297 44613458 44613297 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44612653 44612925 44612653 ID=MS.gene08698.t1.exon6;Parent=MS.gene08698.t1
chr4.2 CDS 44612653 44612925 44612653 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
chr4.2 exon 44612468 44612572 44612468 ID=MS.gene08698.t1.exon7;Parent=MS.gene08698.t1
chr4.2 CDS 44612468 44612572 44612468 ID=cds.MS.gene08698.t1;Parent=MS.gene08698.t1
Gene Sequence

>MS.gene08698

ATGTCAAAAACTCAATTGGGGGTTATTGGTGCACTATTCCTTTCTGTGGCTTCTTCTGTCTCCATTGTCATATGCAACAAAGCCTTAATGAGCAATCTTGGTTTTCCATTCGGTAATTATCATTTTTGTTACTTCTTAAATATGATGTACTTTTCATGATAAAAAATGGTATTTATTTAACTTTATTTTTGTCACATTAATTTGGACCAGCCACAACACTTACAAGTTGGCATTTGATGGTTACTTTTTGCACCCTTCATGTGGCTCTACGTTTCAATCTGTTTGAGGCCAAACCTGTTGACATGAAGACTGTCATGCTTTTTGGCATTCTAAATGGTGTCTCCATTGGATTTCTCAACTTGAGCCTTGGCTTCAATTCCATTGGATTCTATCAGGTTTACTTCAACTTTTTTTTACCACTAGTAATAAATATATTAATTATGGAAAAAAAATGTTAAAAATATTTGGTCCTTTGATGCATGTATCAATATTTTAAAAATCGAACCGAACCATCCAATTTAGCCAATTGAACCGAGAATCGGTTAGTTGGTCAGTTGGTTTGATCCCAATTATTTTGCAGCCTGCTTGAAACGGATTGAATTGGGGTTAAATCGGTTTGGTTGCGGTAGATTCAACTGGTTTTTTTTTTCTTTCATTTTTATACTATTTATTTGTCCTATTTTAAATATATCTTTTTACTTTTATTTGTTGATCATCCTGTTCAACGGTGGTTAAACTGATTGAACAAATAAAATCTTGACCCAAAGGTTTCACCAATTCGATCTTCGGTCCGATTGAAACATTGGTATGCATAATGCATTGAACTACTCATTTGATGTATATATTGATTTTAATGTTTATTTTGTAGATGACAAAACTTGCAATCATACCATTCACAGTTATGCTAGAAACTCTTTTCCTAAAGAAGCAGTTCAGGTAACAATTTAGTTACAAGACACCTAGTGATATTGAAAATAAGTGTGACCATGGTCATGCTAAATTGCTTAATCTGATTGTGAATTATTTTACTACTATGTGCAGCCGGAATATTAAACTCTCTCTATTCTTCTTACTTGTTGGAGTTGGCATTGCTTCTATCACAGACCTTCAACTCAATTTTGTTGGAACCATTCTTTCACTTCTAGCTATCATAACAACATGTGTTGGCCAAATTGTATCCTTTAATGTTCTGTCTTCCTATTGTTAGCATGTTAATATTTTGATGATTTAACTACTCGTGGATTGCTTTATTTGAGCTTATCTACTAGAGAAGCACTTGTGAAACTGTTTGCGAGAGCTTATGGAAACAGCTTATGACAAGTCCATAAGCTCTCTCGGATAACTTATGAAAACTGTTTTAACTTTTATCTTATCTTTCATTATAGAAATTATTTATACATAAACACTTAATTTAGGGCTTGAAAGTTAAAAAGGTTGTTGATTCTTAACTTATTGAACAATTCAGCTCACTAACACAATACAGAAGAAGCTTAATGTCACTTCCACACAACTTCTCTACCAGTCTGCTCCATTCCAAGCAGCAATTCTCTTTGTTTCAGGCCCTCTTGTGGATCGGATGCTAACCAAGCAAAATGTCTTTGCATACAAATATTCTCCTCTTGTCTTGGTAAGTAATGTCAAATTTCAAATTACCCTAATTAATACTAATGTAATGGATAACTAGAAGGATGAGATAAAATTATGTAATATCAACACTTCGATTGAAGACATCTAGTCCAGTGTCTGAAACTAACACGACCCGATACATATTTTTTTTTAACAAGCCACCAATACATAAAGTTAAATTCAATCGCTTTTATTTTCTTAAGTATTAGCGTTAGTGTTATGTCTAGTGTCTGTGTGTCATATAAAATTGTAGATTTGAAACTAAGAATGAAAGCAGCAATATATTTGAAAAACATATATAATTGCTTATACTTATCATATAGAATATGATTATATGACTATATAACTCATATTGTCACCTTTGTATTTCAGACTTTCATCATCATGTCATGTGTGATAGCTGTTTCTGTGAACTTTAGCACCTTTCTAGTAATAGGAAAAACATCTCCAGTAACATATCAAGTGTTGGGCCACCTCAAGACTTGTCTTGTTCTAGGATTTGGCTACACATTGCTCCATGACCCTTTCACTGAAAGGAACATTATTGGAATACTAGTAGCAGTTTTTGGAATGGGTTTGTATTCCTATTTCTGCACTCAAGAGAACAAAAAGAAACTCGTAGTCGATCCTCCTTTGTCGTCTCAGGTAATTTTTTCACCATTTTTCTCACATAGTTTCTCATGTTCATGAGAATTTTGTTGTTTATCTTTGATTATATTTTTCAGGTTAAAGATAAAGATATAAATTCACCACTTTTGAATGGTAAAAACATGGATGAGGAAAAGGAGACTCATGAGTCTAAGAAATCAAACAAGGACTCTCTTGTTTAA
Protein sequence

>MS.gene08698.t1

MSKTQLGVIGALFLSVASSVSIVICNKALMSNLGFPFATTLTSWHLMVTFCTLHVALRFNLFEAKPVDMKTVMLFGILNGVSIGFLNLSLGFNSIGFYQMTKLAIIPFTVMLETLFLKKQFSRNIKLSLFFLLVGVGIASITDLQLNFVGTILSLLAIITTCVGQILTNTIQKKLNVTSTQLLYQSAPFQAAILFVSGPLVDRMLTKQNVFAYKYSPLVLTFIIMSCVIAVSVNFSTFLVIGKTSPVTYQVLGHLKTCLVLGFGYTLLHDPFTERNIIGILVAVFGMGLYSYFCTQENKKKLVVDPPLSSQVKDKDINSPLLNGKNMDEEKETHESKKSNKDSLV