AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85927

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85927.t1 MTR_5g043640 97.765 179 4 0 1 179 69 247 3.20e-126 355
MS.gene85927.t1 MTR_2g048680 36.691 139 88 0 20 158 66 204 4.45e-22 89.4
MS.gene85927.t1 MTR_2g048680 36.220 127 78 3 11 136 90 214 4.53e-18 78.6
MS.gene85927.t1 MTR_2g048680 36.957 138 87 0 21 158 147 284 2.28e-21 89.0
MS.gene85927.t1 MTR_2g048680 36.585 123 75 3 15 136 174 294 2.70e-17 77.8
MS.gene85927.t1 MTR_6g027840 32.704 159 106 1 1 158 156 314 2.38e-17 79.0
MS.gene85927.t1 MTR_6g027840 31.073 177 99 2 3 158 293 467 4.84e-17 78.2
MS.gene85927.t1 MTR_6g027840 32.168 143 93 1 36 178 158 296 7.87e-12 63.2
MS.gene85927.t1 MTR_5g022050 32.609 138 93 0 21 158 258 395 2.93e-17 78.6
MS.gene85927.t1 MTR_5g023090 31.159 138 95 0 21 158 292 429 2.15e-16 76.3
MS.gene85927.t1 MTR_1g011755 32.919 161 75 2 29 156 518 678 3.70e-16 75.9
MS.gene85927.t1 MTR_1g112830 36.641 131 76 4 15 140 228 356 3.87e-16 75.5
MS.gene85927.t1 MTR_1g112830 30.137 146 101 1 14 158 161 306 2.53e-14 70.5
MS.gene85927.t1 MTR_3g491870 41.237 97 57 0 58 154 240 336 4.36e-16 75.1
MS.gene85927.t1 MTR_3g491870 33.607 122 75 2 26 147 241 356 5.59e-16 74.7
MS.gene85927.t1 MTR_3g491870 43.023 86 49 0 5 90 253 338 2.61e-14 70.1
MS.gene85927.t1 MTR_3g009280 37.063 143 83 1 7 142 202 344 5.21e-16 74.7
MS.gene85927.t1 MTR_1g089180 32.667 150 80 1 16 144 280 429 6.20e-16 75.1
MS.gene85927.t1 MTR_1g089180 29.050 179 106 1 1 158 232 410 4.93e-14 69.3
MS.gene85927.t1 MTR_8g056020 34.188 117 77 0 29 145 236 352 1.15e-15 73.9
MS.gene85927.t1 MTR_4g116160 35.659 129 80 2 15 140 129 257 1.58e-15 73.9
MS.gene85927.t1 MTR_4g116160 30.822 146 100 1 14 158 62 207 7.54e-14 68.9
MS.gene85927.t1 MTR_4g116160 32.278 158 104 2 15 169 96 253 9.31e-14 68.6
MS.gene85927.t1 MTR_4g116160 33.588 131 85 2 26 154 40 170 6.32e-13 66.2
MS.gene85927.t1 MTR_4g092760 30.625 160 107 3 15 171 28 186 1.76e-15 73.6
MS.gene85927.t1 MTR_3g107720 32.121 165 79 3 25 156 537 701 1.98e-15 73.6
MS.gene85927.t1 MTR_4g092760 33.333 129 83 2 15 140 116 244 5.05e-15 72.4
MS.gene85927.t1 MTR_1g112820 34.884 129 81 2 15 140 196 324 1.04e-14 71.6
MS.gene85927.t1 MTR_2g096190 34.014 147 92 4 1 144 150 294 2.61e-14 70.5
MS.gene85927.t1 MTR_4g116160 35.659 129 80 2 15 140 129 257 3.22e-14 70.1
MS.gene85927.t1 MTR_4g116160 30.822 146 100 1 14 158 62 207 4.35e-13 67.0
MS.gene85927.t1 MTR_4g116160 32.278 158 104 2 15 169 96 253 6.57e-13 66.2
MS.gene85927.t1 MTR_4g116160 33.588 131 85 2 26 154 40 170 1.36e-12 65.5
MS.gene85927.t1 MTR_5g077770 32.680 153 72 1 19 140 585 737 4.38e-14 69.7
MS.gene85927.t1 MTR_5g077770 31.818 154 99 2 26 179 559 706 2.43e-13 67.8
MS.gene85927.t1 MTR_5g026800 33.071 127 82 1 14 140 37 160 5.42e-14 68.2
MS.gene85927.t1 MTR_5g026800 30.822 146 100 1 26 170 15 160 1.08e-11 62.0
MS.gene85927.t1 MTR_2g006870 28.713 202 111 4 9 177 523 724 7.77e-14 68.9
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 8.97e-14 67.8
MS.gene85927.t1 MTR_7g115280 31.544 149 98 3 11 158 46 191 1.31e-13 68.2
MS.gene85927.t1 MTR_7g115280 34.815 135 87 1 15 149 82 215 4.15e-12 63.9
MS.gene85927.t1 MTR_7g115280 33.083 133 87 2 22 154 122 252 4.40e-12 63.9
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 1.34e-13 67.8
MS.gene85927.t1 MTR_3g085630 34.932 146 86 4 22 158 484 629 1.35e-13 68.2
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 1.51e-13 67.8
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 1.68e-13 67.8
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 2.18e-13 67.4
MS.gene85927.t1 MTR_2g017825 43.678 87 49 0 59 145 42 128 2.34e-13 67.4
MS.gene85927.t1 MTR_5g026550 38.000 100 56 2 19 112 26 125 3.19e-13 64.7
MS.gene85927.t1 MTR_3g088410 40.000 90 54 0 40 129 38 127 4.89e-13 63.9
MS.gene85927.t1 MTR_3g088410 33.663 101 67 0 15 115 46 146 2.42e-11 59.3
MS.gene85927.t1 MTR_6g088470 34.783 161 91 5 24 170 15 175 8.76e-13 65.9
MS.gene85927.t1 MTR_4g113530 31.250 160 78 1 29 156 525 684 1.07e-12 65.9
MS.gene85927.t1 MTR_7g117620 35.938 128 77 4 1 125 165 290 1.45e-12 65.1
MS.gene85927.t1 MTR_7g117620 38.095 126 72 5 1 124 199 320 9.92e-12 62.8
MS.gene85927.t1 MTR_8g088590 39.394 99 57 1 30 128 84 179 2.94e-12 64.3
MS.gene85927.t1 MTR_7g100430 34.375 128 79 4 1 125 173 298 7.42e-12 63.2
MS.gene85927.t1 MTR_5g091880 37.363 91 57 0 59 149 54 144 1.24e-11 62.4
MS.gene85927.t1 MTR_2g101310 34.752 141 86 5 24 158 140 280 1.69e-11 62.0
MS.gene85927.t1 MTR_4g062100 32.653 147 94 4 1 144 154 298 3.15e-11 61.2
MS.gene85927.t1 MTR_4g074170 37.363 91 54 1 22 109 682 772 5.45e-11 60.8
MS.gene85927.t1 MTR_0020s0130 39.535 86 51 1 24 108 47 132 5.98e-11 60.5
MS.gene85927.t1 MTR_4g129470 39.560 91 45 2 48 128 84 174 8.22e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85927.t1 AT2G03430 72.067 179 49 1 1 179 62 239 1.27e-91 267
MS.gene85927.t1 AT2G03430 40.816 147 78 3 19 160 13 155 2.75e-22 90.1
MS.gene85927.t1 AT5G66055 35.766 137 88 0 23 159 261 397 1.20e-21 91.3
MS.gene85927.t1 AT5G66055 33.333 111 74 0 15 125 286 396 8.11e-13 65.9
MS.gene85927.t1 AT4G35450 40.000 115 69 0 26 140 184 298 3.99e-17 77.4
MS.gene85927.t1 AT3G02850 34.437 151 97 2 26 176 552 700 4.60e-17 78.6
MS.gene85927.t1 AT3G02850 34.615 156 71 1 16 140 575 730 3.11e-15 73.2
MS.gene85927.t1 AT3G02850 34.437 151 97 2 26 176 552 700 5.30e-17 78.2
MS.gene85927.t1 AT3G02850 34.615 156 71 1 16 140 575 730 3.41e-15 73.2
MS.gene85927.t1 AT4G35450 40.000 115 69 0 26 140 222 336 6.92e-17 77.0
MS.gene85927.t1 AT4G35450 40.000 115 69 0 26 140 222 336 6.92e-17 77.0
MS.gene85927.t1 AT4G35450 40.000 115 69 0 26 140 222 336 6.92e-17 77.0
MS.gene85927.t1 AT4G35450 40.000 115 69 0 26 140 230 344 7.59e-17 77.0
MS.gene85927.t1 AT4G27780 38.000 100 62 0 26 125 237 336 3.76e-16 75.1
MS.gene85927.t1 AT4G27780 41.346 104 52 1 2 105 246 340 1.09e-14 71.2
MS.gene85927.t1 AT4G27780 33.663 101 67 0 58 158 236 336 2.67e-12 64.3
MS.gene85927.t1 AT2G26650 32.450 151 71 2 37 156 533 683 4.49e-16 75.5
MS.gene85927.t1 AT5G53470 42.857 91 52 0 2 92 231 321 5.29e-16 74.7
MS.gene85927.t1 AT5G53470 38.000 100 62 0 26 125 222 321 2.64e-15 72.8
MS.gene85927.t1 AT5G53470 34.653 101 66 0 58 158 221 321 1.10e-12 65.5
MS.gene85927.t1 AT2G14255 32.530 166 109 2 15 177 84 249 6.15e-16 75.1
MS.gene85927.t1 AT2G14255 31.469 143 92 3 1 140 106 245 1.59e-12 65.1
MS.gene85927.t1 AT2G14255 27.586 145 83 3 26 158 28 162 3.87e-12 63.9
MS.gene85927.t1 AT2G26650 32.450 151 71 2 37 156 428 578 7.89e-16 74.7
MS.gene85927.t1 AT4G32500 33.333 159 75 1 29 156 549 707 1.26e-15 74.3
MS.gene85927.t1 AT2G17390 37.391 115 72 0 26 140 224 338 3.01e-15 72.4
MS.gene85927.t1 AT5G40160 28.261 138 99 0 21 158 151 288 4.41e-15 72.0
MS.gene85927.t1 AT5G40160 29.286 140 90 4 15 147 178 315 8.05e-13 65.5
MS.gene85927.t1 AT5G37500 30.769 156 77 1 16 140 558 713 6.23e-15 72.4
MS.gene85927.t1 AT5G37500 37.383 107 65 1 14 118 521 627 3.01e-13 67.4
MS.gene85927.t1 AT2G25600 32.716 162 78 1 29 159 551 712 7.28e-15 72.0
MS.gene85927.t1 AT2G25600 28.931 159 82 1 16 143 571 729 1.33e-11 62.8
MS.gene85927.t1 AT1G07710 36.735 147 88 4 1 144 150 294 9.51e-15 71.6
MS.gene85927.t1 AT1G07710 36.735 147 88 4 1 144 150 294 9.51e-15 71.6
MS.gene85927.t1 AT4G19150 32.727 110 74 0 15 124 43 152 1.27e-14 69.7
MS.gene85927.t1 AT5G02620 34.783 138 87 3 9 143 112 249 2.13e-14 70.5
MS.gene85927.t1 AT5G02620 34.783 138 87 3 9 143 112 249 2.13e-14 70.5
MS.gene85927.t1 AT5G02620 34.783 138 87 3 9 143 112 249 2.13e-14 70.5
MS.gene85927.t1 AT5G20350 32.895 152 96 4 15 162 124 273 2.18e-14 70.5
MS.gene85927.t1 AT5G20350 30.323 155 105 2 5 158 50 202 2.40e-14 70.5
MS.gene85927.t1 AT5G20350 33.333 135 88 2 26 158 35 169 4.46e-14 69.7
MS.gene85927.t1 AT5G20350 30.588 170 113 3 15 179 91 260 5.03e-13 66.6
MS.gene85927.t1 AT5G40160 28.571 126 90 0 21 146 151 276 2.46e-14 69.7
MS.gene85927.t1 AT5G40160 30.579 121 77 3 15 134 178 292 2.29e-13 67.0
MS.gene85927.t1 AT5G02620 37.903 124 74 3 9 129 112 235 2.79e-14 70.5
MS.gene85927.t1 AT5G02620 29.843 191 120 6 1 178 71 260 5.58e-11 60.8
MS.gene85927.t1 AT5G02620 37.903 124 74 3 9 129 112 235 2.79e-14 70.5
MS.gene85927.t1 AT5G02620 29.843 191 120 6 1 178 71 260 5.58e-11 60.8
MS.gene85927.t1 AT3G04710 35.443 158 97 3 1 158 104 256 3.08e-14 70.1
MS.gene85927.t1 AT3G04710 33.582 134 87 2 26 158 59 191 2.86e-13 67.4
MS.gene85927.t1 AT3G04710 35.075 134 86 1 16 149 83 215 2.97e-13 67.4
MS.gene85927.t1 AT3G04710 33.803 142 85 3 26 158 18 159 5.72e-12 63.5
MS.gene85927.t1 AT3G04710 35.443 158 97 3 1 158 328 480 4.77e-14 69.7
MS.gene85927.t1 AT3G04710 33.582 134 87 2 26 158 283 415 3.50e-13 67.0
MS.gene85927.t1 AT3G04710 35.075 134 86 1 16 149 307 439 4.23e-13 67.0
MS.gene85927.t1 AT3G04710 33.803 142 85 3 26 158 242 383 7.31e-12 63.2
MS.gene85927.t1 AT5G37500 34.127 126 81 1 15 140 558 681 7.69e-14 68.9
MS.gene85927.t1 AT5G37500 34.127 126 81 1 15 140 612 735 7.77e-14 68.9
MS.gene85927.t1 AT3G04710 33.582 134 87 2 26 158 59 191 2.80e-13 67.4
MS.gene85927.t1 AT3G04710 34.868 152 94 3 1 152 104 250 9.54e-13 65.9
MS.gene85927.t1 AT3G04710 33.803 142 85 3 26 158 18 159 5.50e-12 63.5
MS.gene85927.t1 AT5G61230 37.000 100 57 2 19 112 26 125 9.00e-13 63.5
MS.gene85927.t1 AT4G19150 32.323 99 67 0 26 124 31 129 3.35e-12 62.8
MS.gene85927.t1 AT5G13530 32.576 132 82 3 51 179 507 634 4.67e-12 63.9
MS.gene85927.t1 AT5G13530 32.576 132 82 3 51 179 507 634 4.67e-12 63.9
MS.gene85927.t1 AT1G10870 42.391 92 50 1 21 109 682 773 4.73e-12 63.9
MS.gene85927.t1 AT1G10870 42.391 92 50 1 21 109 687 778 4.78e-12 63.9
MS.gene85927.t1 AT5G12320 35.644 101 65 0 29 129 18 118 4.98e-12 60.8
MS.gene85927.t1 AT5G66055 33.333 99 66 0 23 121 261 359 6.16e-12 63.2
MS.gene85927.t1 AT4G22200 32.824 131 86 2 28 158 547 675 2.05e-11 62.0
MS.gene85927.t1 AT3G28880 30.573 157 100 3 12 160 15 170 2.78e-11 61.6
MS.gene85927.t1 AT3G59830 35.593 118 74 1 6 123 62 177 3.49e-11 61.2
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 57 139 4.07e-11 60.8
MS.gene85927.t1 AT3G58760 39.080 87 53 0 59 145 53 139 9.30e-11 60.1
MS.gene85927.t1 AT5G13300 37.079 89 54 1 39 125 711 799 4.13e-11 61.2
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 57 139 4.59e-11 60.8
MS.gene85927.t1 AT2G31800 37.374 99 59 1 30 128 83 178 4.88e-11 60.8
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 120 202 4.95e-11 60.8
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 120 202 4.95e-11 60.8
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 120 202 5.44e-11 60.8
MS.gene85927.t1 AT3G58760 37.349 83 52 0 30 112 120 202 5.44e-11 60.8
MS.gene85927.t1 AT2G43850 39.362 94 57 0 30 123 86 179 5.97e-11 60.5
MS.gene85927.t1 AT2G43850 39.362 94 57 0 30 123 86 179 6.14e-11 60.5
MS.gene85927.t1 AT2G43850 39.362 94 57 0 30 123 86 179 6.26e-11 60.5
MS.gene85927.t1 AT5G60070 31.783 129 81 4 22 144 177 304 6.44e-11 60.5
MS.gene85927.t1 AT5G07840 34.000 100 60 2 19 112 28 127 9.40e-11 58.2

Find 48 sgRNAs with CRISPR-Local

Find 277 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATATTAATGTTGGCATTA+TGG 0.170106 5.3:+31079231 None:intergenic
CATTGGGAATTCGGAGATTC+TGG 0.207112 5.3:-31081077 MS.gene85927:CDS
AATATTAATGTTGGCATTAT+GGG 0.246874 5.3:+31079232 None:intergenic
AATCTTCATCCGACCTTTGC+TGG 0.277498 5.3:+31079271 None:intergenic
TAATGCCAACATTAATATTA+AGG 0.292110 5.3:-31079228 MS.gene85927:CDS
AGGTAGCACTCCTTCTTATA+AGG 0.293510 5.3:-31076939 MS.gene85927:CDS
TGCTGCCAGCATTGGGAATT+CGG 0.328342 5.3:-31081086 MS.gene85927:CDS
TGCTAGCTGCCCGATGCAAC+GGG 0.338078 5.3:+31079050 None:intergenic
TTGCATCGGGCAGCTAGCAC+TGG 0.385320 5.3:-31079046 MS.gene85927:CDS
ATTCATTCTGCTGCCAGCAT+TGG 0.404125 5.3:-31081094 MS.gene85927:CDS
TTCATTCTGCTGCCAGCATT+GGG 0.422094 5.3:-31081093 MS.gene85927:CDS
TGGTTCCACCCCGTTGCATC+GGG 0.426096 5.3:-31079059 MS.gene85927:CDS
CAAATCACGGCACTCATTAG+AGG 0.434441 5.3:+31078950 None:intergenic
TTGGTTCCACCCCGTTGCAT+CGG 0.439880 5.3:-31079060 MS.gene85927:CDS
GTATCATTCAAGCATGGCCT+TGG 0.445360 5.3:+31076822 None:intergenic
CTTGTCCTTAATATTAATGT+TGG 0.455131 5.3:+31079223 None:intergenic
GTGCTAGCTGCCCGATGCAA+CGG 0.466543 5.3:+31079049 None:intergenic
CTGCTCCATGCCTTATAAGA+AGG 0.504054 5.3:+31076929 None:intergenic
GTGGATGTGGAGGACAAGGA+AGG 0.514850 5.3:-31076904 MS.gene85927:CDS
TTTGCTGGCTGCATAGTGTA+AGG 0.518159 5.3:+31079286 None:intergenic
CTCTTTGTTATAACAAATCA+CGG 0.520454 5.3:+31078937 None:intergenic
GTGTTTGTATCATTCAAGCA+TGG 0.530236 5.3:+31076816 None:intergenic
AAGGAAGGGTACACGGTGCT+TGG 0.531206 5.3:-31076889 MS.gene85927:CDS
CGTGATTTGTTATAACAAAG+AGG 0.532092 5.3:-31078936 MS.gene85927:intron
AATCTCCGAATTCCCAATGC+TGG 0.540552 5.3:+31081081 None:intergenic
TGCATCGGGCAGCTAGCACT+GGG 0.548238 5.3:-31079045 MS.gene85927:CDS
ATAAATAGTGGTGATGAAGA+AGG 0.550982 5.3:-31081127 MS.gene85927:CDS
CTGGAGGCTTTGTTGAACAA+AGG 0.556222 5.3:-31081058 MS.gene85927:intron
GCACTCCTTCTTATAAGGCA+TGG 0.559998 5.3:-31076934 MS.gene85927:CDS
TATAAGGCATGGAGCAGATG+TGG 0.564074 5.3:-31076923 MS.gene85927:CDS
AGATGTGGATGTGGAGGACA+AGG 0.564628 5.3:-31076908 MS.gene85927:CDS
CAACATTAATATTAAGGACA+AGG 0.567959 5.3:-31079222 MS.gene85927:intron
GTTGATGCTGTTGATAGAGC+TGG 0.574506 5.3:-31078980 MS.gene85927:CDS
AATATTGATTGATGCTGCCA+AGG 0.587026 5.3:-31076839 MS.gene85927:CDS
TTACACTATGCAGCCAGCAA+AGG 0.589849 5.3:-31079284 MS.gene85927:CDS
TAGTGGTGATGAAGAAGGGT+GGG 0.590265 5.3:-31081122 MS.gene85927:CDS
GCATGGAGCAGATGTGGATG+TGG 0.603022 5.3:-31076917 MS.gene85927:CDS
ATGCTGGCAGCAGAATGAAT+CGG 0.606631 5.3:+31081097 None:intergenic
TCTGCTGAAGTGATAAATAG+TGG 0.609473 5.3:-31081139 MS.gene85927:CDS
ATAGTGGTGATGAAGAAGGG+TGG 0.609755 5.3:-31081123 MS.gene85927:CDS
TAAATAGTGGTGATGAAGAA+GGG 0.612103 5.3:-31081126 MS.gene85927:CDS
AGCTGCCCGATGCAACGGGG+TGG 0.618931 5.3:+31079054 None:intergenic
ACTATGCAGCCAGCAAAGGT+CGG 0.624620 5.3:-31079280 MS.gene85927:CDS
TGGGAATTCGGAGATTCTGG+AGG 0.634438 5.3:-31081074 MS.gene85927:CDS
GGAGGACAAGGAAGGGTACA+CGG 0.639207 5.3:-31076896 MS.gene85927:CDS
TGGATGTGGAGGACAAGGAA+GGG 0.643078 5.3:-31076903 MS.gene85927:CDS
TGGAGCAGATGTGGATGTGG+AGG 0.658645 5.3:-31076914 MS.gene85927:CDS
GCTAGCTGCCCGATGCAACG+GGG 0.716203 5.3:+31079051 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGTTAATATTAAAAACAAT+GGG - chr5.3:31078675-31078694 MS.gene85927:intron 10.0%
!! ATTCCAAAAATAAAATAAAA+TGG - chr5.3:31080655-31080674 MS.gene85927:intron 10.0%
!!! AATCCATTTTATTTTATTTT+TGG + chr5.3:31080661-31080680 None:intergenic 10.0%
!! AATGTGATTATAATGAAAAT+GGG + chr5.3:31078856-31078875 None:intergenic 15.0%
!! ATAAAAATCTATTATGTGTT+TGG - chr5.3:31081027-31081046 MS.gene85927:intron 15.0%
!! GATGTTAATATTAAAAACAA+TGG - chr5.3:31078674-31078693 MS.gene85927:intron 15.0%
!! TAATGTGATTATAATGAAAA+TGG + chr5.3:31078857-31078876 None:intergenic 15.0%
!! TATGATAAATTATACTCAAT+CGG + chr5.3:31079505-31079524 None:intergenic 15.0%
!! TGTTAATATTAAAAACAATG+GGG - chr5.3:31078676-31078695 MS.gene85927:intron 15.0%
!!! AAAAAGAACATAATTTTACA+TGG + chr5.3:31080360-31080379 None:intergenic 15.0%
!!! AAAAGAACATAATTTTACAT+GGG + chr5.3:31080359-31080378 None:intergenic 15.0%
!!! AATAAATTGATTACAAGTTT+TGG - chr5.3:31079533-31079552 MS.gene85927:intron 15.0%
!! AATAAGGTAAAAATAGCTTA+CGG + chr5.3:31077527-31077546 None:intergenic 20.0%
!! AATATTAATGTTGGCATTAT+GGG + chr5.3:31078765-31078784 None:intergenic 20.0%
!! AATTTAGTTAGTTAGTTAGT+TGG + chr5.3:31078393-31078412 None:intergenic 20.0%
!! ATAATCACTTGTACTATAAA+TGG - chr5.3:31077192-31077211 MS.gene85927:intron 20.0%
!! ATATCCTAACAGTAATAATT+TGG - chr5.3:31078482-31078501 MS.gene85927:intron 20.0%
!! ATGGAACTATTTGTATAAAT+AGG + chr5.3:31078326-31078345 None:intergenic 20.0%
!! ATTAACTTCTGATAGATTTA+GGG - chr5.3:31077249-31077268 MS.gene85927:intron 20.0%
!! ATTTAGTTAGTTAGTTAGTT+GGG + chr5.3:31078392-31078411 None:intergenic 20.0%
!! GAAAACAAAATAATAGAAAC+TGG + chr5.3:31080597-31080616 None:intergenic 20.0%
!! GTTAATATTAAAAACAATGG+GGG - chr5.3:31078677-31078696 MS.gene85927:intron 20.0%
!! TAATATTAATGTTGGCATTA+TGG + chr5.3:31078766-31078785 None:intergenic 20.0%
!! TAATGCCAACATTAATATTA+AGG - chr5.3:31078766-31078785 MS.gene85927:intron 20.0%
!! TATCCTAACAGTAATAATTT+GGG - chr5.3:31078483-31078502 MS.gene85927:intron 20.0%
!! TCTTCTATTTAGTACTTAAA+AGG - chr5.3:31080724-31080743 MS.gene85927:intron 20.0%
!!! AATCATTCATATTAAGCTTT+TGG - chr5.3:31079569-31079588 MS.gene85927:intron 20.0%
!!! ATTTGTTTCCATTTTTAATG+AGG - chr5.3:31080508-31080527 MS.gene85927:intron 20.0%
!!! TAAGAGATTTAGAATCTATT+TGG - chr5.3:31076987-31077006 MS.gene85927:intron 20.0%
!!! TAATCGTATCAATTTTGTTT+TGG - chr5.3:31080254-31080273 MS.gene85927:intron 20.0%
!!! TTTAAGATAATATAACAGTG+TGG + chr5.3:31080452-31080471 None:intergenic 20.0%
!!! TTTGTTTCCATTTTTAATGA+GGG - chr5.3:31080509-31080528 MS.gene85927:intron 20.0%
! AAGTACAATAATTAGATGAG+TGG - chr5.3:31077796-31077815 MS.gene85927:intron 25.0%
! ATAATGTGAAGAAATGTTGT+TGG + chr5.3:31079843-31079862 None:intergenic 25.0%
! CAACATTAATATTAAGGACA+AGG - chr5.3:31078772-31078791 MS.gene85927:intron 25.0%
! CAGAATTATATAATTTCCCT+TGG + chr5.3:31080898-31080917 None:intergenic 25.0%
! CATAGACATTTGTAATTGTT+AGG - chr5.3:31078111-31078130 MS.gene85927:intron 25.0%
! CATTAACTTCTGATAGATTT+AGG - chr5.3:31077248-31077267 MS.gene85927:intron 25.0%
! CGATTAAAAAAGAAACTGAT+CGG + chr5.3:31080240-31080259 None:intergenic 25.0%
! CTAAAGATCTTATGGAAATA+AGG + chr5.3:31077543-31077562 None:intergenic 25.0%
! CTTATTTCCATAAGATCTTT+AGG - chr5.3:31077541-31077560 MS.gene85927:intron 25.0%
! CTTGTCCTTAATATTAATGT+TGG + chr5.3:31078774-31078793 None:intergenic 25.0%
! GAAAACAAAATCACAATCTT+TGG + chr5.3:31079348-31079367 None:intergenic 25.0%
! TAATGTGAAGAAATGTTGTT+GGG + chr5.3:31079842-31079861 None:intergenic 25.0%
! TATAGAAAAGTGGTATAGAA+TGG + chr5.3:31078465-31078484 None:intergenic 25.0%
! TGATCATATTACAGTTTACA+AGG + chr5.3:31078912-31078931 None:intergenic 25.0%
! TTCATATCCTAAAGATCTTA+TGG + chr5.3:31077551-31077570 None:intergenic 25.0%
! TTGTAAACTGTAATATGATC+AGG - chr5.3:31078911-31078930 MS.gene85927:intron 25.0%
! TTTCATTTGTGATTATCTGT+TGG - chr5.3:31077446-31077465 MS.gene85927:intron 25.0%
! TTTCCCAAATTATTACTGTT+AGG + chr5.3:31078489-31078508 None:intergenic 25.0%
!! ACGTTCTTATTCTTCATTTT+TGG - chr5.3:31078643-31078662 MS.gene85927:intron 25.0%
!! ACTTATTTGAGTTTATCGAT+TGG - chr5.3:31077014-31077033 MS.gene85927:intron 25.0%
!! AGTAAACCTTTAAGAACTAT+GGG + chr5.3:31078034-31078053 None:intergenic 25.0%
!! CTAAGATGAGAAATATGTTT+TGG - chr5.3:31080949-31080968 MS.gene85927:intron 25.0%
!! CTCTTTGTTATAACAAATCA+CGG + chr5.3:31079060-31079079 None:intergenic 25.0%
!! GTTCTTACAATTTTTATCGT+AGG - chr5.3:31079105-31079124 MS.gene85927:intron 25.0%
!! TATTGGAGTCTTCATAATTT+TGG - chr5.3:31080981-31081000 MS.gene85927:intron 25.0%
!! TCTTTTGAATTTAATTCCCT+GGG + chr5.3:31078138-31078157 None:intergenic 25.0%
!! TTCTTTTGAATTTAATTCCC+TGG + chr5.3:31078139-31078158 None:intergenic 25.0%
!!! GTTGGACTTTATTTTAAGAA+AGG - chr5.3:31077752-31077771 MS.gene85927:intron 25.0%
!!! TATGCTTTTTTATCGAAACA+GGG - chr5.3:31077614-31077633 MS.gene85927:intron 25.0%
!!! TATTAAGCTTTTGGAATAGA+TGG - chr5.3:31079578-31079597 MS.gene85927:intron 25.0%
!!! TATTTTAAGAAAGGTTCATG+TGG - chr5.3:31077761-31077780 MS.gene85927:intron 25.0%
!!! TCAACTTTTATTTCAAGTCA+CGG + chr5.3:31079671-31079690 None:intergenic 25.0%
!!! TCTTATTCTTCATTTTTGGT+AGG - chr5.3:31078647-31078666 MS.gene85927:intron 25.0%
!!! TTATGCTTTTTTATCGAAAC+AGG - chr5.3:31077613-31077632 MS.gene85927:intron 25.0%
AAACACTTGTCAGATTGTTT+GGG - chr5.3:31077472-31077491 MS.gene85927:intron 30.0%
AAACTGTAATATGATCAGGT+TGG - chr5.3:31078915-31078934 MS.gene85927:intron 30.0%
AACTTAACAGTCTCAACTAA+AGG + chr5.3:31077713-31077732 None:intergenic 30.0%
AACTTGTAAGACGTTATAGA+AGG - chr5.3:31080767-31080786 MS.gene85927:intron 30.0%
AAGAATAAGAACGTTCAAGA+TGG + chr5.3:31078637-31078656 None:intergenic 30.0%
AAGCTCTCATGAAAAGTTTA+TGG + chr5.3:31077084-31077103 None:intergenic 30.0%
AATCCATTCTTTAGGTATAC+AGG + chr5.3:31080629-31080648 None:intergenic 30.0%
AATTGGTGCAAAACTACATT+TGG + chr5.3:31079990-31080009 None:intergenic 30.0%
ACTGTTAGGATATAGAAAAG+TGG + chr5.3:31078475-31078494 None:intergenic 30.0%
AGCTTAGTATGCTTAACTTT+GGG - chr5.3:31080405-31080424 MS.gene85927:intron 30.0%
AGTTAGTTGGGATTGTTATA+GGG + chr5.3:31078380-31078399 None:intergenic 30.0%
ATACTGTCAATCCATTCTTT+AGG + chr5.3:31080637-31080656 None:intergenic 30.0%
ATCCATTCTTTAGGTATACA+GGG + chr5.3:31080628-31080647 None:intergenic 30.0%
ATTGGTGCAAAACTACATTT+GGG + chr5.3:31079989-31080008 None:intergenic 30.0%
GTCAAAAAGTTTAAGCAAAC+AGG - chr5.3:31079903-31079922 MS.gene85927:intron 30.0%
TAAACACTTGTCAGATTGTT+TGG - chr5.3:31077471-31077490 MS.gene85927:intron 30.0%
TAGTTAGTTGGGATTGTTAT+AGG + chr5.3:31078381-31078400 None:intergenic 30.0%
TGAATCTTTCTATCTTCAGA+TGG + chr5.3:31078610-31078629 None:intergenic 30.0%
TTTAGTTGAGACTGTTAAGT+TGG - chr5.3:31077712-31077731 MS.gene85927:intron 30.0%
! AACCATTGATTGACCATTAA+CGG - chr5.3:31079154-31079173 MS.gene85927:intron 30.0%
! ATAAATAGTGGTGATGAAGA+AGG - chr5.3:31076867-31076886 MS.gene85927:CDS 30.0%
! ATTTTGTTCCAAATGCAATC+TGG - chr5.3:31080321-31080340 MS.gene85927:intron 30.0%
! CAGTAAACCTTTAAGAACTA+TGG + chr5.3:31078035-31078054 None:intergenic 30.0%
! CTATTATGTGTTTGGTTTAC+AGG - chr5.3:31081035-31081054 MS.gene85927:intron 30.0%
! GTTTAGTTTAACTTAAGCGT+TGG - chr5.3:31077734-31077753 MS.gene85927:intron 30.0%
! TAAATAGTGGTGATGAAGAA+GGG - chr5.3:31076868-31076887 MS.gene85927:CDS 30.0%
! TAGCTAGTTTAGTTAGTTGA+GGG + chr5.3:31078353-31078372 None:intergenic 30.0%
! TGATAGATTTAGGGTATGTT+TGG - chr5.3:31077258-31077277 MS.gene85927:intron 30.0%
! TTAGCTAGTTTAGTTAGTTG+AGG + chr5.3:31078354-31078373 None:intergenic 30.0%
!! CAACTTTTATTTCAAGTCAC+GGG + chr5.3:31079670-31079689 None:intergenic 30.0%
!! CAGTTGATTTTTCAATCAAC+GGG - chr5.3:31079238-31079257 MS.gene85927:CDS 30.0%
!! CGTGATTTGTTATAACAAAG+AGG - chr5.3:31079058-31079077 MS.gene85927:CDS 30.0%
!! GCAGTTGATTTTTCAATCAA+CGG - chr5.3:31079237-31079256 MS.gene85927:CDS 30.0%
!! TCCATTTTTAATGAGGGTAA+GGG - chr5.3:31080515-31080534 MS.gene85927:intron 30.0%
!! TGTGAACTTTTGATTGAAGA+AGG - chr5.3:31078984-31079003 MS.gene85927:CDS 30.0%
!! TTCCATTTTTAATGAGGGTA+AGG - chr5.3:31080514-31080533 MS.gene85927:intron 30.0%
!! TTGTCGTGTTTGTGTTTTTA+TGG - chr5.3:31077946-31077965 MS.gene85927:intron 30.0%
!!! CTTTTCAGGTATATGGTTTA+TGG - chr5.3:31079805-31079824 MS.gene85927:intron 30.0%
!!! TGTTTTGGTAGTTCTGTTAT+TGG - chr5.3:31080964-31080983 MS.gene85927:intron 30.0%
AATAGAAGAAAGCAGTAAGC+TGG + chr5.3:31080713-31080732 None:intergenic 35.0%
ACCCTTACCCTCATTAAAAA+TGG + chr5.3:31080519-31080538 None:intergenic 35.0%
ACTGAAAGAGGATATTACAG+AGG + chr5.3:31079963-31079982 None:intergenic 35.0%
AGTAGTAACTCTACTGTGAA+GGG + chr5.3:31078247-31078266 None:intergenic 35.0%
ATTTAGTTGCACAGTGCTAA+GGG - chr5.3:31079319-31079338 MS.gene85927:CDS 35.0%
ATTTGTAATTGTTAGGACCC+AGG - chr5.3:31078118-31078137 MS.gene85927:intron 35.0%
CAGCTTAGTATGCTTAACTT+TGG - chr5.3:31080404-31080423 MS.gene85927:intron 35.0%
CTAGTACCCATAGTTCTTAA+AGG - chr5.3:31078025-31078044 MS.gene85927:intron 35.0%
CTGAAAGAGGATATTACAGA+GGG + chr5.3:31079962-31079981 None:intergenic 35.0%
CTGCATTCAATCAAGTTACA+CGG + chr5.3:31078004-31078023 None:intergenic 35.0%
GATATTACAGAGGGATGAAA+TGG + chr5.3:31079953-31079972 None:intergenic 35.0%
GCTAGGTAAAGACATATGTA+TGG + chr5.3:31079780-31079799 None:intergenic 35.0%
GGTATGTTTGGATTGATTGA+GGG - chr5.3:31077270-31077289 MS.gene85927:intron 35.0%
GTGGAATTATAAACCATGAG+AGG + chr5.3:31079464-31079483 None:intergenic 35.0%
GTTAAACACAAGAAATCCCT+CGG - chr5.3:31080197-31080216 MS.gene85927:intron 35.0%
GTTGAAAACAAAACCTCTCA+TGG - chr5.3:31079448-31079467 MS.gene85927:intron 35.0%
TAAAGATCTGTTTGGGAGTA+GGG + chr5.3:31077405-31077424 None:intergenic 35.0%
TAAGTGATGAGATGATGTGT+TGG + chr5.3:31080564-31080583 None:intergenic 35.0%
TAATTGTACTGTGAGCTAAG+TGG - chr5.3:31077675-31077694 MS.gene85927:intron 35.0%
TACTCCCAAACAGATCTTTA+GGG - chr5.3:31077405-31077424 MS.gene85927:intron 35.0%
TAGAAGGTTGAAAATGCAGT+TGG - chr5.3:31080783-31080802 MS.gene85927:intron 35.0%
TATTTAGTTGCACAGTGCTA+AGG - chr5.3:31079318-31079337 MS.gene85927:CDS 35.0%
TCTGCTGAAGTGATAAATAG+TGG - chr5.3:31076855-31076874 MS.gene85927:CDS 35.0%
TCTTTCTATCTTCAGATGGA+AGG + chr5.3:31078606-31078625 None:intergenic 35.0%
TTAGATCCTTTATGACCAAG+TGG - chr5.3:31079615-31079634 MS.gene85927:intron 35.0%
TTTGTAATTGTTAGGACCCA+GGG - chr5.3:31078119-31078138 MS.gene85927:intron 35.0%
! AATATTGATTGATGCTGCCA+AGG - chr5.3:31081155-31081174 MS.gene85927:CDS 35.0%
! AATTTTTCCATCGTTGTTGG+GGG - chr5.3:31080006-31080025 MS.gene85927:intron 35.0%
! ACCAATTTTTCCATCGTTGT+TGG - chr5.3:31080003-31080022 MS.gene85927:intron 35.0%
! ATACCTGAAAAGCTTTAGCT+AGG + chr5.3:31079797-31079816 None:intergenic 35.0%
! CAATTTTTCCATCGTTGTTG+GGG - chr5.3:31080005-31080024 MS.gene85927:intron 35.0%
! CATAATTTTACATGGGAGAC+TGG + chr5.3:31080352-31080371 None:intergenic 35.0%
! CCAATTTTTCCATCGTTGTT+GGG - chr5.3:31080004-31080023 MS.gene85927:intron 35.0%
! TTACCTAGCTAAAGCTTTTC+AGG - chr5.3:31079791-31079810 MS.gene85927:intron 35.0%
! TTTTTAATGAGGGTAAGGGT+AGG - chr5.3:31080519-31080538 MS.gene85927:intron 35.0%
!! TTAGTTAGTTGAGGGTGTTA+TGG + chr5.3:31078345-31078364 None:intergenic 35.0%
!!! AACTTTTAGTTTCAACACCG+AGG - chr5.3:31080864-31080883 MS.gene85927:intron 35.0%
!!! ATTGATTGAGGGTTTTGAAG+GGG - chr5.3:31077281-31077300 MS.gene85927:intron 35.0%
!!! GATTGATTGAGGGTTTTGAA+GGG - chr5.3:31077280-31077299 MS.gene85927:intron 35.0%
!!! GCTAAAGCTTTTCAGGTATA+TGG - chr5.3:31079798-31079817 MS.gene85927:intron 35.0%
!!! TTTGTTTTAGTCACTTGCAC+TGG - chr5.3:31079382-31079401 MS.gene85927:intron 35.0%
AAGTTGAATTTCAGCGCACA+TGG - chr5.3:31079684-31079703 MS.gene85927:intron 40.0%
ACAGACCCTAAAGATCTGTT+TGG + chr5.3:31077413-31077432 None:intergenic 40.0%
ACTAACGATTTGACCATGAG+TGG + chr5.3:31080161-31080180 None:intergenic 40.0%
AGAAGATGCTGCTAGACATT+AGG + chr5.3:31077640-31077659 None:intergenic 40.0%
AGACGTTGTGGTTGTGTAAA+TGG + chr5.3:31079882-31079901 None:intergenic 40.0%
AGGTAGCACTCCTTCTTATA+AGG - chr5.3:31081055-31081074 MS.gene85927:intron 40.0%
ATAGCTTACGGACATGTCAT+CGG + chr5.3:31077515-31077534 None:intergenic 40.0%
ATTGATTGACCATTAACGGC+AGG - chr5.3:31079158-31079177 MS.gene85927:intron 40.0%
CAGACCCTAAAGATCTGTTT+GGG + chr5.3:31077412-31077431 None:intergenic 40.0%
CCCAACAACGATGGAAAAAT+TGG + chr5.3:31080007-31080026 None:intergenic 40.0%
CTAAAGATCTGTTTGGGAGT+AGG + chr5.3:31077406-31077425 None:intergenic 40.0%
CTACTCCCAAACAGATCTTT+AGG - chr5.3:31077404-31077423 MS.gene85927:intron 40.0%
CTCCCTGTATACCTAAAGAA+TGG - chr5.3:31080623-31080642 MS.gene85927:intron 40.0%
CTCTTAGAACTTAGAAGCCT+TGG - chr5.3:31079926-31079945 MS.gene85927:intron 40.0%
GAAACTGATCGGTTAGAACA+CGG + chr5.3:31080229-31080248 None:intergenic 40.0%
GAAAGAAATCTCTCCTCCAA+GGG - chr5.3:31078217-31078236 MS.gene85927:intron 40.0%
GAACACTTGTGAGACTGTTT+GGG - chr5.3:31077040-31077059 MS.gene85927:intron 40.0%
GACAATGCAACACAGAAACA+TGG + chr5.3:31077925-31077944 None:intergenic 40.0%
GAGAGAGAGAGAGTAAGAAT+AGG - chr5.3:31078195-31078214 MS.gene85927:intron 40.0%
GAGTAGTAACTCTACTGTGA+AGG + chr5.3:31078248-31078267 None:intergenic 40.0%
GCATACTAAGCTGTTACAGA+TGG + chr5.3:31080397-31080416 None:intergenic 40.0%
GCATTTCGTTTATAGCAAGG+TGG + chr5.3:31079483-31079502 None:intergenic 40.0%
GGGTATGTTTGGATTGATTG+AGG - chr5.3:31077269-31077288 MS.gene85927:intron 40.0%
GTAGTAACTCTACTGTGAAG+GGG + chr5.3:31078246-31078265 None:intergenic 40.0%
GTATTGAACTCAAGACCACT+TGG + chr5.3:31079633-31079652 None:intergenic 40.0%
TCAAGACCACTTGGTCATAA+AGG + chr5.3:31079624-31079643 None:intergenic 40.0%
TCGGCATTTCGTTTATAGCA+AGG + chr5.3:31079486-31079505 None:intergenic 40.0%
TCTTCAGATGGAAGGATTGA+CGG + chr5.3:31078598-31078617 None:intergenic 40.0%
TGAACACTTGTGAGACTGTT+TGG - chr5.3:31077039-31077058 MS.gene85927:intron 40.0%
TGCCGTTAATGGTCAATCAA+TGG + chr5.3:31079159-31079178 None:intergenic 40.0%
TTAAGGACAAGGTTGTAGCT+TGG - chr5.3:31078783-31078802 MS.gene85927:intron 40.0%
TTGATTGACCATTAACGGCA+GGG - chr5.3:31079159-31079178 MS.gene85927:intron 40.0%
! AACTTTTTGACGAGACGTTG+TGG + chr5.3:31079894-31079913 None:intergenic 40.0%
! ACATTTGGGACTACTGAAAG+AGG + chr5.3:31079975-31079994 None:intergenic 40.0%
! AGTAAGCTGGAGTCTTGTAA+AGG + chr5.3:31080700-31080719 None:intergenic 40.0%
! GTTAGTTTGAGTCAGCAATC+AGG - chr5.3:31080175-31080194 MS.gene85927:intron 40.0%
!! ACAGATCTTTAGGGTCTGTT+TGG - chr5.3:31077414-31077433 MS.gene85927:intron 40.0%
!! GGATTGATTGAGGGTTTTGA+AGG - chr5.3:31077279-31077298 MS.gene85927:intron 40.0%
AAATGAGCAGTACGCCTTTG+AGG + chr5.3:31079282-31079301 None:intergenic 45.0%
AACCTTCACTTTCCCCTCAA+AGG - chr5.3:31079265-31079284 MS.gene85927:CDS 45.0%
AATCTCCGAATTCCCAATGC+TGG + chr5.3:31076916-31076935 None:intergenic 45.0%
AATCTTCATCCGACCTTTGC+TGG + chr5.3:31078726-31078745 None:intergenic 45.0%
AATGAGCAGTACGCCTTTGA+GGG + chr5.3:31079281-31079300 None:intergenic 45.0%
ATCACAATCCCTGCCGTTAA+TGG + chr5.3:31079170-31079189 None:intergenic 45.0%
ATGCTGGCAGCAGAATGAAT+CGG + chr5.3:31076900-31076919 None:intergenic 45.0%
ATTCATTCTGCTGCCAGCAT+TGG - chr5.3:31076900-31076919 MS.gene85927:CDS 45.0%
ATTGCGGAATTGCAGAGTGA+CGG - chr5.3:31080088-31080107 MS.gene85927:intron 45.0%
CAAATCACGGCACTCATTAG+AGG + chr5.3:31079047-31079066 None:intergenic 45.0%
CATTGGGAATTCGGAGATTC+TGG - chr5.3:31076917-31076936 MS.gene85927:CDS 45.0%
CTATGTGTTTGTGTCGTGTC+AGG + chr5.3:31078096-31078115 None:intergenic 45.0%
CTCAGTGTTTGCCTAAGATG+TGG - chr5.3:31080287-31080306 MS.gene85927:intron 45.0%
CTGCTCCATGCCTTATAAGA+AGG + chr5.3:31081068-31081087 None:intergenic 45.0%
GCACTCCTTCTTATAAGGCA+TGG - chr5.3:31081060-31081079 MS.gene85927:CDS 45.0%
GGAAAGAAATCTCTCCTCCA+AGG - chr5.3:31078216-31078235 MS.gene85927:intron 45.0%
GGAATAGATGGTTCGTGACA+TGG - chr5.3:31079590-31079609 MS.gene85927:intron 45.0%
TATAAGGCATGGAGCAGATG+TGG - chr5.3:31081071-31081090 MS.gene85927:CDS 45.0%
TCGAGTGCATACCACATCTT+AGG + chr5.3:31080301-31080320 None:intergenic 45.0%
TGCGTTTGTGCTCTATGCTT+CGG - chr5.3:31079712-31079731 MS.gene85927:intron 45.0%
TTACACTATGCAGCCAGCAA+AGG - chr5.3:31078710-31078729 MS.gene85927:intron 45.0%
TTCATTCTGCTGCCAGCATT+GGG - chr5.3:31076901-31076920 MS.gene85927:CDS 45.0%
! ATAGTGGTGATGAAGAAGGG+TGG - chr5.3:31076871-31076890 MS.gene85927:CDS 45.0%
! ATTTTACATGGGAGACTGGC+CGG + chr5.3:31080348-31080367 None:intergenic 45.0%
! GTTGATGCTGTTGATAGAGC+TGG - chr5.3:31079014-31079033 MS.gene85927:CDS 45.0%
! TAGTGGTGATGAAGAAGGGT+GGG - chr5.3:31076872-31076891 MS.gene85927:CDS 45.0%
! TCTGTTTGGGAGTAGGGTTT+TGG + chr5.3:31077399-31077418 None:intergenic 45.0%
! TTTGCTGGCTGCATAGTGTA+AGG + chr5.3:31078711-31078730 None:intergenic 45.0%
!! CTGGAGGCTTTGTTGAACAA+AGG - chr5.3:31076936-31076955 MS.gene85927:CDS 45.0%
!!! ATTGAGGGTTTTGAAGGGGA+GGG - chr5.3:31077285-31077304 MS.gene85927:intron 45.0%
!!! TTTGGGAGTAGGGTTTTGGA+GGG + chr5.3:31077395-31077414 None:intergenic 45.0%
ACTATGCAGCCAGCAAAGGT+CGG - chr5.3:31078714-31078733 MS.gene85927:intron 50.0%
ACTGATCGGTTAGAACACGG+AGG + chr5.3:31080226-31080245 None:intergenic 50.0%
AGATGTGGATGTGGAGGACA+AGG - chr5.3:31081086-31081105 MS.gene85927:CDS 50.0%
AGTGGCGTGATCGACAACAT+AGG + chr5.3:31080058-31080077 None:intergenic 50.0%
AGTTTCAACACCGAGGTGCA+AGG - chr5.3:31080871-31080890 MS.gene85927:intron 50.0%
ATGAGCAGTACGCCTTTGAG+GGG + chr5.3:31079280-31079299 None:intergenic 50.0%
CACAAGTGTCAGTGTCGTGT+CGG - chr5.3:31078530-31078549 MS.gene85927:intron 50.0%
CAGAGGGATGAAATGGTCCA+AGG + chr5.3:31079946-31079965 None:intergenic 50.0%
CTTGTGAGACTGTTTGGGAG+AGG - chr5.3:31077045-31077064 MS.gene85927:intron 50.0%
GCAGACCTACTCATCAGTCA+AGG - chr5.3:31080028-31080047 MS.gene85927:intron 50.0%
GCATAGAGCACAAACGCACT+CGG + chr5.3:31079710-31079729 None:intergenic 50.0%
GCGTTTGTGCTCTATGCTTC+GGG - chr5.3:31079713-31079732 MS.gene85927:intron 50.0%
GTGTTGGACATTGGTCGTGT+CGG - chr5.3:31078552-31078571 MS.gene85927:intron 50.0%
GTTCCAAATGCAATCTGGCC+CGG - chr5.3:31080326-31080345 MS.gene85927:intron 50.0%
GTTTCAACACCGAGGTGCAA+GGG - chr5.3:31080872-31080891 MS.gene85927:intron 50.0%
TACTGTGAAGGGGAAACCCT+TGG + chr5.3:31078236-31078255 None:intergenic 50.0%
TAGAACACGGAGGTTTCCGA+GGG + chr5.3:31080216-31080235 None:intergenic 50.0%
TCCGCAATTGCAAGCCAAAG+TGG + chr5.3:31080076-31080095 None:intergenic 50.0%
TGGATGTGGAGGACAAGGAA+GGG - chr5.3:31081091-31081110 MS.gene85927:CDS 50.0%
TGGGAATTCGGAGATTCTGG+AGG - chr5.3:31076920-31076939 MS.gene85927:CDS 50.0%
TGTTGTCGATCACGCCACTT+TGG - chr5.3:31080059-31080078 MS.gene85927:intron 50.0%
TTAGAACACGGAGGTTTCCG+AGG + chr5.3:31080217-31080236 None:intergenic 50.0%
TTAGGTATACAGGGAGAGCG+AGG + chr5.3:31080619-31080638 None:intergenic 50.0%
! ATTTCAAGTCACGGGCAGCA+AGG + chr5.3:31079662-31079681 None:intergenic 50.0%
! GTCAGGTGTGTGTGGGTTAT+TGG + chr5.3:31078079-31078098 None:intergenic 50.0%
! TGCTGCCAGCATTGGGAATT+CGG - chr5.3:31076908-31076927 MS.gene85927:CDS 50.0%
!! GCCACTTTGGCTTGCAATTG+CGG - chr5.3:31080072-31080091 MS.gene85927:intron 50.0%
!! GTTTGGGAGTAGGGTTTTGG+AGG + chr5.3:31077396-31077415 None:intergenic 50.0%
!! TTTTACATGGGAGACTGGCC+GGG + chr5.3:31080347-31080366 None:intergenic 50.0%
!!! GATTGAGGGTTTTGAAGGGG+AGG - chr5.3:31077284-31077303 MS.gene85927:intron 50.0%
CTCTATGCTTCGGGTGTGTG+TGG - chr5.3:31079722-31079741 MS.gene85927:intron 55.0%
GCATGGAGCAGATGTGGATG+TGG - chr5.3:31081077-31081096 MS.gene85927:CDS 55.0%
GCGCACCTTGACTGATGAGT+AGG + chr5.3:31080036-31080055 None:intergenic 55.0%
GTGGATGTGGAGGACAAGGA+AGG - chr5.3:31081090-31081109 MS.gene85927:CDS 55.0%
GTGTCGTGTCAGGTGTGTGT+GGG + chr5.3:31078086-31078105 None:intergenic 55.0%
TGGAGCAGATGTGGATGTGG+AGG - chr5.3:31081080-31081099 MS.gene85927:CDS 55.0%
TGTGAAGGGGAAACCCTTGG+AGG + chr5.3:31078233-31078252 None:intergenic 55.0%
TGTGTCGTGTCAGGTGTGTG+TGG + chr5.3:31078087-31078106 None:intergenic 55.0%
TTGGTTCCACCCCGTTGCAT+CGG - chr5.3:31078934-31078953 MS.gene85927:intron 55.0%
! AAGGAAGGGTACACGGTGCT+TGG - chr5.3:31081105-31081124 MS.gene85927:CDS 55.0%
! CGCCTTTGAGGGGAAAGTGA+AGG + chr5.3:31079270-31079289 None:intergenic 55.0%
! GACATTGGTCGTGTCGGTGT+TGG - chr5.3:31078558-31078577 MS.gene85927:intron 55.0%
! GGAGGACAAGGAAGGGTACA+CGG - chr5.3:31081098-31081117 MS.gene85927:CDS 55.0%
! TGTCAGTGTCGTGTCGGTGT+TGG - chr5.3:31078536-31078555 MS.gene85927:intron 55.0%
!! GTCGTGTCGGTGTTGGACAT+TGG - chr5.3:31078543-31078562 MS.gene85927:intron 55.0%
AGGTCTGCCCCCAACAACGA+TGG + chr5.3:31080016-31080035 None:intergenic 60.0%
AGTGGCACGCCTCCTGAATG+CGG + chr5.3:31080143-31080162 None:intergenic 60.0%
GCCTCCTGAATGCGGCTCAT+CGG + chr5.3:31080135-31080154 None:intergenic 60.0%
GTGCTAGCTGCCCGATGCAA+CGG + chr5.3:31078948-31078967 None:intergenic 60.0%
TCAGCCGATGAGCCGCATTC+AGG - chr5.3:31080128-31080147 MS.gene85927:intron 60.0%
TGCATCGGGCAGCTAGCACT+GGG - chr5.3:31078949-31078968 MS.gene85927:CDS 60.0%
TGCTAGCTGCCCGATGCAAC+GGG + chr5.3:31078947-31078966 None:intergenic 60.0%
TGGCCGGGCCAGATTGCATT+TGG + chr5.3:31080332-31080351 None:intergenic 60.0%
TGGTTCCACCCCGTTGCATC+GGG - chr5.3:31078935-31078954 MS.gene85927:intron 60.0%
TTCCCTTGGCCCTTGCACCT+CGG + chr5.3:31080884-31080903 None:intergenic 60.0%
TTGCATCGGGCAGCTAGCAC+TGG - chr5.3:31078948-31078967 MS.gene85927:CDS 60.0%
! TTCAGGAGGCGTGCCACTCA+TGG - chr5.3:31080145-31080164 MS.gene85927:intron 60.0%
ACACCGAGGTGCAAGGGCCA+AGG - chr5.3:31080878-31080897 MS.gene85927:intron 65.0%
CACCGAGGTGCAAGGGCCAA+GGG - chr5.3:31080879-31080898 MS.gene85927:intron 65.0%
GCCGATGAGCCGCATTCAGG+AGG - chr5.3:31080131-31080150 MS.gene85927:intron 65.0%
GCTAGCTGCCCGATGCAACG+GGG + chr5.3:31078946-31078965 None:intergenic 65.0%
AGCTGCCCGATGCAACGGGG+TGG + chr5.3:31078943-31078962 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 31076825 31081191 31076825 ID=MS.gene85927
chr5.3 mRNA 31076825 31081191 31076825 ID=MS.gene85927.t1;Parent=MS.gene85927
chr5.3 exon 31081059 31081191 31081059 ID=MS.gene85927.t1.exon1;Parent=MS.gene85927.t1
chr5.3 CDS 31081059 31081191 31081059 ID=cds.MS.gene85927.t1;Parent=MS.gene85927.t1
chr5.3 exon 31079223 31079347 31079223 ID=MS.gene85927.t1.exon2;Parent=MS.gene85927.t1
chr5.3 CDS 31079223 31079347 31079223 ID=cds.MS.gene85927.t1;Parent=MS.gene85927.t1
chr5.3 exon 31078937 31079083 31078937 ID=MS.gene85927.t1.exon3;Parent=MS.gene85927.t1
chr5.3 CDS 31078937 31079083 31078937 ID=cds.MS.gene85927.t1;Parent=MS.gene85927.t1
chr5.3 exon 31076825 31076959 31076825 ID=MS.gene85927.t1.exon4;Parent=MS.gene85927.t1
chr5.3 CDS 31076825 31076959 31076825 ID=cds.MS.gene85927.t1;Parent=MS.gene85927.t1
Gene Sequence

>MS.gene85927

GTGGTGAAGATATTGTTGTCTTGTGATGCATCTGCTGAAGTGATAAATAGTGGTGATGAAGAAGGGTGGGCACCGATTCATTCTGCTGCCAGCATTGGGAATTCGGAGATTCTGGAGGCTTTGTTGAACAAAGGTATATTTTTATGTTATTTTATTAACTTTTAAGAGATTTAGAATCTATTTGGATTGACTTATTTGAGTTTATCGATTGGCATGAACACTTGTGAGACTGTTTGGGAGAGGTTTCAACATATTTCCATAAACTTTTCATGAGAGCTTGTGAAAACAACTTATAGCTTATACGAAAACAGTTAGACTTAATTTTATCTTCTGTTGTAGAAATAGTTTGTACATAAGCACTTATATGATAATCACTTGTACTATAAATGGTTCGTTGAACAAAAGACTTTTTTTATTTTACTTCATTAACTTCTGATAGATTTAGGGTATGTTTGGATTGATTGAGGGTTTTGAAGGGGAGGGTTTAGTTTTCTTTTTCAAATTAAGAACGCTTGAAGTATTATATATCATGTTGTTCTTTCAAAAAATATCAACATATAGACTTAACCCTCCAAAACCCTACTCCCAAACAGATCTTTAGGGTCTGTTTGGATTGACTTATTTCATTTGTGATTATCTGTTGGTATAAACACTTGTCAGATTGTTTGGGAGAGCTAATGAAAACAACCGATGACATGTCCGTAAGCTATTTTTACCTTATTTCCATAAGATCTTTAGGATATGAAAACAACTATCTTTTGTTATATGAAAAACTTGTATATAAGCACTTATGCTTTTTTATCGAAACAGGGCCTAATGTCTAGCAGCATCTTCTATTTTTTGTGAACATTAATTGTACTGTGAGCTAAGTGGTTTATGTGTGTTCCTTTAGTTGAGACTGTTAAGTTGGTTTAGTTTAACTTAAGCGTTGGACTTTATTTTAAGAAAGGTTCATGTGGACTTGTATCAGAAAGTACAATAATTAGATGAGTGGTGCTATATTATTATGTTAACAATGATTTTCATACAAACTTGTCAAACATTTATTTTCACTTTATAGTTGTGTCAAGTGCAATATTGTTTAGACTTTGTTGTGTCCATGTTTCTGTGTTGCATTGTCTTTGTCGTGTTTGTGTTTTTATGGTTGTAAACTGCTGCTTTCTCTTAATTTTTCTTCCGTGTAACTTGATTGAATGCAGTCTAGTACCCATAGTTCTTAAAGGTTTACTGATCTAGTAATAAGATGCAATACCAATAACCCACACACACCTGACACGACACAAACACATAGACATTTGTAATTGTTAGGACCCAGGGAATTAAATTCAAAAGAAAGTACAATTTTATTGAAAGATTATGAAAGAATAAGAGAGAGAGAGAGAGTAAGAATAGGAAAGAAATCTCTCCTCCAAGGGTTTCCCCTTCACAGTAGAGTTACTACTCTGTTCACAATGTTTACAATATTCATTCAATAATGATCAACTCTACATTGTGTTCTCCTATTTATACAAATAGTTCCATAACACCCTCAACTAACTAAACTAGCTAACTGCCCTATAACAATCCCAACTAACTAACTAACTAAATTCTGTAAGATTATAACAAATCTTAACTACTTTAACTACTCTAATTAAGCACCATTCTATACCACTTTTCTATATCCTAACAGTAATAATTTGGGAAAATAGAAGTGATTGTATGTAATCACAAGTGTCAGTGTCGTGTCGGTGTTGGACATTGGTCGTGTCGGTGTTGGACATTGACACATGTCCGTCAATCCTTCCATCTGAAGATAGAAAGATTCATCAACCATCTTGAACGTTCTTATTCTTCATTTTTGGTAGGAGCTGATGTTAATATTAAAAACAATGGGGGACGCACTGCCTTACACTATGCAGCCAGCAAAGGTCGGATGAAGATTGCTGAAATACTCATCTCCCATAATGCCAACATTAATATTAAGGACAAGGTTGTAGCTTGGATTCATTTGATATATTTGTGAATTTTGACAATATTGTTTCAATGCTTCCCATTTTCATTATAATCACATTATTAAAGATGACATCATATTAATTTAGTGTATACCTTGTAAACTGTAATATGATCAGGTTGGTTCCACCCCGTTGCATCGGGCAGCTAGCACTGGGAATTCTGAGTTGTGTGAACTTTTGATTGAAGAAGGAGCTGATGTTGATGCTGTTGATAGAGCTGGTCAAACTCCTCTAATGAGTGCCGTGATTTGTTATAACAAAGAGGTATGCTATTTTTATCTGTACATGTGTTCTTACAATTTTTATCGTAGGAGTAAACTAGTAAAGTGTAGCATTACAACCATTGATTGACCATTAACGGCAGGGATTGTGATTAAGTTATTGTTCATATAATGCAAACAATGAAGTTATTAAAATATTAGCAGTTGATTTTTCAATCAACGGGTATAAACCTTCACTTTCCCCTCAAAGGCGTACTGCTCATTTAAAAAAAAAAAAAAACTATTTAGTTGCACAGTGCTAAGGGTTACCAAAGATTGTGATTTTGTTTTCAACTAAATAATTTTTTTGTTTTAGTCACTTGCACTGGCATGCATTGTCGTAATAAGATGTGAAATAATTTGCTATATGCTGTTGAAAACAAAACCTCTCATGGTTTATAATTCCACCTTGCTATAAACGAAATGCCGATTGAGTATAATTTATCATAATTATATAAATAAATTGATTACAAGTTTTGGAAATATTATATTAAATCATTCATATTAAGCTTTTGGAATAGATGGTTCGTGACATGGTGTTAGATCCTTTATGACCAAGTGGTCTTGAGTTCAATACTTCGATCCTTGCTGCCCGTGACTTGAAATAAAAGTTGAATTTCAGCGCACATGGCCGAGTGCGTTTGTGCTCTATGCTTCGGGTGTGTGTGGAGACATGTTAGAAATATAATATAAAACAAAAACCATACATATGTCTTTACCTAGCTAAAGCTTTTCAGGTATATGGTTTATGGCATCAAAATATCCCAACAACATTTCTTCACATTATATTCTATCTGAAATCACCATTTACACAACCACAACGTCTCGTCAAAAAGTTTAAGCAAACAGGCTCTTAGAACTTAGAAGCCTTGGACCATTTCATCCCTCTGTAATATCCTCTTTCAGTAGTCCCAAATGTAGTTTTGCACCAATTTTTCCATCGTTGTTGGGGGCAGACCTACTCATCAGTCAAGGTGCGCCTATGTTGTCGATCACGCCACTTTGGCTTGCAATTGCGGAATTGCAGAGTGACGGCATCTTCTGCGACGAGTTCAGCCGATGAGCCGCATTCAGGAGGCGTGCCACTCATGGTCAAATCGTTAGTTTGAGTCAGCAATCAGGTTAAACACAAGAAATCCCTCGGAAACCTCCGTGTTCTAACCGATCAGTTTCTTTTTTAATCGTATCAATTTTGTTTTGGTTGCTTTAGTCTCAGTGTTTGCCTAAGATGTGGTATGCACTCGAATTTTGTTCCAAATGCAATCTGGCCCGGCCAGTCTCCCATGTAAAATTATGTTCTTTTTCTCTTGCAACTGTTCCATCTGTAACAGCTTAGTATGCTTAACTTTGGGTAGAATATATGAAAAATATTTCCACACTGTTATATTATCTTAAATATTGTAACAGTTTTCATATTTATTTGTATATTTTGATTTGTTTCCATTTTTAATGAGGGTAAGGGTAGGATTTCAGTATTTTTATTGTCCAACACATCATCTCATCACTTAACTATCAATTCCAGTTTCTATTATTTTGTTTTCCTCGCTCTCCCTGTATACCTAAAGAATGGATTGACAGTATTCCAAAAATAAAATAAAATGGATTGACACGTCTAATGTGTCCTTTACAAGACTCCAGCTTACTGCTTTCTTCTATTTAGTACTTAAAAGGAAGTTCTGCTTAGTAGTTTTAACTTGTAAGACGTTATAGAAGGTTGAAAATGCAGTTGGAGACATGCAACAAATTCCATCATACTGTTTAGTATTCAGAGTATCATATGCTATCTGAAACTTTTAGTTTCAACACCGAGGTGCAAGGGCCAAGGGAAATTATATAATTCTGTAATGTGTATGTACATAGAGTAGATAATAGTCTAAGATGAGAAATATGTTTTGGTAGTTCTGTTATTGGAGTCTTCATAATTTTGGAGCAGCGTACATTGATGTAGTGCATAAAAATCTATTATGTGTTTGGTTTACAGGTAGCACTCCTTCTTATAAGGCATGGAGCAGATGTGGATGTGGAGGACAAGGAAGGGTACACGGTGCTTGGTCGAATTACTGACGATGAGTTTAGATCAATATTGATTGATGCTGCCAAGGCCATGCTTGAATGA
Protein sequence

>MS.gene85927.t1

VVKILLSCDASAEVINSGDEEGWAPIHSAASIGNSEILEALLNKGADVNIKNNGGRTALHYAASKGRMKIAEILISHNANINIKDKVGSTPLHRAASTGNSELCELLIEEGADVDAVDRAGQTPLMSAVICYNKEVALLLIRHGADVDVEDKEGYTVLGRITDDEFRSILIDAAKAMLE