AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08700

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08700.t1 MTR_4g058910 95.312 320 15 0 1 320 83 402 0.0 601
MS.gene08700.t1 MTR_2g094600 54.717 318 120 7 1 318 8 301 2.29e-102 303
MS.gene08700.t1 MTR_1g094930 39.252 214 101 6 132 316 23 236 1.85e-35 129
MS.gene08700.t1 MTR_1g094930 39.720 214 100 6 132 316 118 331 2.62e-35 130
MS.gene08700.t1 MTR_1g116010 78.462 65 14 0 232 296 164 228 1.21e-33 124
MS.gene08700.t1 MTR_4g071590 72.059 68 19 0 231 298 210 277 3.22e-32 122
MS.gene08700.t1 MTR_4g471590 72.059 68 19 0 231 298 210 277 3.22e-32 122
MS.gene08700.t1 MTR_1g046680 72.222 72 20 0 232 303 172 243 3.26e-32 120
MS.gene08700.t1 MTR_3g117380 69.444 72 22 0 227 298 224 295 1.30e-31 120
MS.gene08700.t1 MTR_5g007600 66.279 86 28 1 213 298 241 325 2.32e-31 120
MS.gene08700.t1 MTR_5g007600 63.750 80 29 0 216 295 141 220 1.19e-27 110
MS.gene08700.t1 MTR_6g032830 58.333 96 40 0 201 296 161 256 3.26e-31 118
MS.gene08700.t1 MTR_8g013950 65.432 81 28 0 218 298 212 292 3.38e-31 120
MS.gene08700.t1 MTR_8g076340 68.000 75 22 1 232 306 218 290 3.59e-31 119
MS.gene08700.t1 MTR_7g085410 71.831 71 20 0 229 299 185 255 4.39e-31 118
MS.gene08700.t1 MTR_1g006650 73.134 67 18 0 232 298 234 300 5.92e-31 119
MS.gene08700.t1 MTR_5g021340 68.000 75 22 1 232 306 300 372 2.33e-30 119
MS.gene08700.t1 MTR_3g109760 73.134 67 18 0 232 298 207 273 1.44e-29 115
MS.gene08700.t1 MTR_7g446110 35.374 147 86 1 147 293 111 248 1.28e-22 95.1
MS.gene08700.t1 MTR_7g445950 53.247 77 36 0 217 293 105 181 1.70e-22 92.8
MS.gene08700.t1 MTR_7g446070 51.948 77 37 0 217 293 117 193 1.19e-21 90.9
MS.gene08700.t1 MTR_7g445980 51.250 80 38 1 215 293 16 95 9.80e-20 82.8
MS.gene08700.t1 MTR_5g020230 36.145 83 47 2 186 264 74 154 4.20e-12 65.5
MS.gene08700.t1 MTR_7g112330 57.500 40 17 0 228 267 18 57 6.74e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08700.t1 AT1G08010 35.032 314 152 10 8 302 10 290 7.92e-49 165
MS.gene08700.t1 AT1G08010 35.032 314 152 10 8 302 10 290 7.92e-49 165
MS.gene08700.t1 AT1G08010 35.032 314 152 10 8 302 10 290 7.92e-49 165
MS.gene08700.t1 AT1G08000 36.000 300 164 8 8 301 10 287 4.64e-44 153
MS.gene08700.t1 AT1G08000 36.000 300 164 8 8 301 10 287 4.64e-44 153
MS.gene08700.t1 AT2G28340 33.117 308 144 8 5 302 6 261 2.26e-35 130
MS.gene08700.t1 AT3G54810 77.778 72 16 0 227 298 224 295 1.59e-34 128
MS.gene08700.t1 AT3G54810 77.778 72 16 0 227 298 224 295 1.59e-34 128
MS.gene08700.t1 AT3G24050 71.429 70 20 0 229 298 191 260 2.47e-31 119
MS.gene08700.t1 AT4G36240 42.767 159 69 4 138 296 92 228 6.36e-31 117
MS.gene08700.t1 AT3G60530 69.014 71 22 0 232 302 158 228 7.03e-31 117
MS.gene08700.t1 AT4G32890 68.056 72 23 0 227 298 192 263 7.11e-31 118
MS.gene08700.t1 AT5G25830 71.642 67 19 0 232 298 219 285 2.34e-30 117
MS.gene08700.t1 AT2G45050 53.000 100 44 1 202 298 146 245 3.37e-30 115
MS.gene08700.t1 AT5G66320 66.216 74 25 0 225 298 242 315 4.25e-30 117
MS.gene08700.t1 AT5G66320 66.216 74 25 0 225 298 242 315 4.25e-30 117
MS.gene08700.t1 AT3G51080 65.789 76 24 1 232 307 221 294 1.54e-29 115
MS.gene08700.t1 AT4G34680 60.976 82 30 1 229 308 177 258 2.44e-29 113
MS.gene08700.t1 AT4G34680 60.976 82 30 1 229 308 177 258 2.44e-29 113
MS.gene08700.t1 AT3G45170 62.857 70 26 0 229 298 112 181 3.29e-28 108
MS.gene08700.t1 AT2G18380 54.545 44 19 1 221 264 82 124 4.40e-11 62.0

Find 67 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCATCGAAGGAGACATTT+TGG 0.229278 4.2:+44593034 None:intergenic
GAGAAATTGTTCACAAGATT+TGG 0.237962 4.2:+44594723 None:intergenic
TATTTATCATCAAACCTTTC+AGG 0.244075 4.2:-44592457 MS.gene08700:CDS
CAAGCATTGCATAGGGTCTT+AGG 0.290975 4.2:+44592634 None:intergenic
CTAGCTGCAGGCCGATATTC+AGG 0.293194 4.2:+44592580 None:intergenic
GTTAATCAGGAGACTGTTAA+AGG 0.309270 4.2:-44592493 MS.gene08700:CDS
CAGGCCGATATTCAGGAAAT+AGG 0.331499 4.2:+44592587 None:intergenic
GGTCTTGGAAATCAGGAAAA+AGG 0.338584 4.2:-44592515 MS.gene08700:CDS
ACAAGAAGGTCTTGGAAATC+AGG 0.346966 4.2:-44592522 MS.gene08700:CDS
GTCGCCTATTTCCTGAATAT+CGG 0.393892 4.2:-44592591 MS.gene08700:CDS
TGGGAGATATTGTCTTTCCT+TGG 0.402736 4.2:+44592772 None:intergenic
CTGAAACTGGGCTGTAGGTT+CGG 0.407286 4.2:+44592995 None:intergenic
TACCACGAGGACGCTTTGTC+GGG 0.437518 4.2:+44592914 None:intergenic
AAATTCTCACAAGAAGGTCT+TGG 0.447787 4.2:-44592530 MS.gene08700:CDS
TGCACTGATGCTTCAAAGGT+TGG 0.449929 4.2:+44592556 None:intergenic
TCAAAGGTTGGGCTAGCTGC+AGG 0.450155 4.2:+44592568 None:intergenic
GGCGCCTCTCAAGCTTCGAC+AGG 0.458075 4.2:-44592885 MS.gene08700:CDS
ATGCTTGTGGAGTTCGATAC+AGG 0.460657 4.2:-44592618 MS.gene08700:CDS
CATAGGGTCTTAGGACCCTT+AGG 0.477379 4.2:+44592643 None:intergenic
TCTCAATGCTATTAGAGAGC+TGG 0.477442 4.2:+44592752 None:intergenic
GGACGCTTTGTCGGGATCAC+TGG 0.489854 4.2:+44592922 None:intergenic
CACCAAGAGATGGTTCTTCA+AGG 0.491420 4.2:+44594796 None:intergenic
GGAAACTGATGGTGTGGAAC+AGG 0.492036 4.2:-44594841 MS.gene08700:CDS
CTCAATGCTATTAGAGAGCT+GGG 0.500421 4.2:+44592753 None:intergenic
AAGACTGCTGGACCTGCATA+TGG 0.501903 4.2:-44593072 MS.gene08700:CDS
TTACCACGAGGACGCTTTGT+CGG 0.503422 4.2:+44592913 None:intergenic
CACCTTGAAGAACCATCTCT+TGG 0.504280 4.2:-44594798 MS.gene08700:CDS
GCACTGATGCTTCAAAGGTT+GGG 0.507344 4.2:+44592557 None:intergenic
CAATGGAGAGAGGGGCCTAA+GGG 0.517972 4.2:-44592658 MS.gene08700:CDS
GGTGTGTCGGATGAGATTCT+TGG 0.520640 4.2:-44594903 MS.gene08700:CDS
CTGCTAATATGAAGAACAGC+TGG 0.521475 4.2:-44594947 None:intergenic
AAACAGCTTGGAAAGACTGC+TGG 0.521813 4.2:-44593084 MS.gene08700:intron
TGTGGAGTTCGATACAGGTC+TGG 0.526380 4.2:-44592613 MS.gene08700:CDS
ACAATGGAGAGAGGGGCCTA+AGG 0.527062 4.2:-44592659 MS.gene08700:CDS
TGAAAGCAGTAGTTATTCCT+CGG 0.529501 4.2:-44592968 MS.gene08700:CDS
ATCCAAAATGTCTCCTTCGA+TGG 0.535212 4.2:-44593036 MS.gene08700:CDS
AAACCTTTCAGGAATTCTCT+AGG 0.536952 4.2:-44592446 None:intergenic
GCGTAAATTTGTTCCATCGA+AGG 0.553548 4.2:+44593023 None:intergenic
CTGATGGTGTGGAACAGGAT+TGG 0.558249 4.2:-44594836 MS.gene08700:CDS
GAACTCCACAAGCATTGCAT+AGG 0.559486 4.2:+44592626 None:intergenic
TGAAACGCCACAATGGAGAG+AGG 0.562131 4.2:-44592668 MS.gene08700:CDS
GCACTCAAATTCTCACAAGA+AGG 0.564610 4.2:-44592536 MS.gene08700:CDS
TGAGTGCACTGATGCTTCAA+AGG 0.568513 4.2:+44592552 None:intergenic
CAATCGGATAAACAGCGTGC+TGG 0.568743 4.2:-44592823 MS.gene08700:CDS
TGTCGAAGCTTGAGAGGCGC+CGG 0.571004 4.2:+44592887 None:intergenic
AGAGCCTGTCGAAGCTTGAG+AGG 0.571997 4.2:+44592881 None:intergenic
TCAGTTTCCACATCTTCAAG+TGG 0.590091 4.2:+44594855 None:intergenic
AACTCCACAAGCATTGCATA+GGG 0.590903 4.2:+44592627 None:intergenic
TATCCTAGAGAATTCCTGAA+AGG 0.593357 4.2:+44592443 None:intergenic
AAGACCCTATGCAATGCTTG+TGG 0.595397 4.2:-44592631 MS.gene08700:CDS
TTAGGCCCCTCTCTCCATTG+TGG 0.615985 4.2:+44592661 None:intergenic
GAAACGCCACAATGGAGAGA+GGG 0.617261 4.2:-44592667 MS.gene08700:CDS
TAGAAAATGTACGCATTGTG+AGG 0.619646 4.2:-44592695 MS.gene08700:CDS
TCGCCGCGTAGCTCAAATGC+CGG 0.621988 4.2:+44594762 None:intergenic
AGCGTCCTCGTGGTAAACGC+CGG 0.626063 4.2:-44592906 MS.gene08700:CDS
CTTGAAGATGTGGAAACTGA+TGG 0.650171 4.2:-44594852 MS.gene08700:CDS
ACTGAGAAAACACCAAGAGA+TGG 0.656666 4.2:+44594786 None:intergenic
AAAGTTGTAATTCTCAACCG+AGG 0.667884 4.2:+44592951 None:intergenic
ATCCCGACAAAGCGTCCTCG+TGG 0.667975 4.2:-44592916 MS.gene08700:CDS
AGATGTGGAAACTGATGGTG+TGG 0.668716 4.2:-44594847 MS.gene08700:CDS
GTTTGATGATAAATACAACA+TGG 0.669284 4.2:+44592465 None:intergenic
AAACGCCACAATGGAGAGAG+GGG 0.674715 4.2:-44592666 MS.gene08700:CDS
AGGTAACTGAAACGCCACAA+TGG 0.680689 4.2:-44592675 MS.gene08700:CDS
TTCGATGGAACAAATTTACG+CGG 0.690434 4.2:-44593021 MS.gene08700:CDS
AGGCGCCGGCGTTTACCACG+AGG 0.716581 4.2:+44592901 None:intergenic
GTACCGGCATTTGAGCTACG+CGG 0.750665 4.2:-44594765 MS.gene08700:CDS
TCAAGCTTAAAAGATCGACG+AGG 0.787048 4.2:-44594306 MS.gene08700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATATTTTTTTAAAATAA+GGG - chr4.2:44593939-44593958 MS.gene08700:intron 0.0%
!! AATTATTATATGTTTAGAGA+CGG - chr4.2:44593970-44593989 MS.gene08700:intron 15.0%
!!! ATATTTTTTTAAAATAAGGG+AGG - chr4.2:44593942-44593961 MS.gene08700:intron 15.0%
!!! ATGTGTTATAAAACTTTTTT+GGG - chr4.2:44593199-44593218 MS.gene08700:intron 15.0%
!!! TAATGTTTTAATTAAACTCA+AGG + chr4.2:44594198-44594217 None:intergenic 15.0%
!!! TATTTTTTTAAAATAAGGGA+GGG - chr4.2:44593943-44593962 MS.gene08700:intron 15.0%
!!! TTTACTTTTATGATACATTT+TGG - chr4.2:44593351-44593370 MS.gene08700:intron 15.0%
!!! TTTTTTGACAAGAATTTTAA+CGG - chr4.2:44592462-44592481 MS.gene08700:CDS 15.0%
!! ATTATTATATGTTTAGAGAC+GGG - chr4.2:44593971-44593990 MS.gene08700:intron 20.0%
!! TGATAAATTAATTATGTGCA+TGG - chr4.2:44592761-44592780 MS.gene08700:CDS 20.0%
!!! ATTTTTTTAAAATAAGGGAG+GGG - chr4.2:44593944-44593963 MS.gene08700:intron 20.0%
!!! GATGTGTTATAAAACTTTTT+TGG - chr4.2:44593198-44593217 MS.gene08700:intron 20.0%
!!! TGTGTTATAAAACTTTTTTG+GGG - chr4.2:44593200-44593219 MS.gene08700:intron 20.0%
!!! TTCTTTTTGAAATGTTTTCT+AGG - chr4.2:44593163-44593182 MS.gene08700:intron 20.0%
! ATACTTACAACTTCAAAACA+TGG + chr4.2:44593624-44593643 None:intergenic 25.0%
! CTCTGATAATAAAAAATGTG+TGG + chr4.2:44592834-44592853 None:intergenic 25.0%
! GCTCAAAATATGTTCAAAAA+AGG - chr4.2:44593431-44593450 MS.gene08700:intron 25.0%
! TATTTATCATCAAACCTTTC+AGG - chr4.2:44594929-44594948 MS.gene08700:CDS 25.0%
!! AAAGTATGGAAGAATTTTAG+TGG - chr4.2:44594169-44594188 MS.gene08700:intron 25.0%
!! GTTTGATGATAAATACAACA+TGG + chr4.2:44594924-44594943 None:intergenic 25.0%
!! TCTTCTTAGACGTTTTTAAT+TGG - chr4.2:44592930-44592949 MS.gene08700:CDS 25.0%
!! TTCGTGTATGATTAATTTTC+AGG - chr4.2:44593478-44593497 MS.gene08700:intron 25.0%
!!! GTTTAAGTATCTTTTTGACT+CGG - chr4.2:44593140-44593159 MS.gene08700:intron 25.0%
!!! GTTTTTCAAATAAAACAGCT+TGG - chr4.2:44594290-44594309 MS.gene08700:intron 25.0%
!!! TTTTAGTTGTGATGAATAGA+TGG - chr4.2:44593643-44593662 MS.gene08700:intron 25.0%
AAAGGCTAATTCGTTTGATA+GGG - chr4.2:44592881-44592900 MS.gene08700:CDS 30.0%
AAATCTGTCTCAAATTCTCT+TGG - chr4.2:44593534-44593553 MS.gene08700:intron 30.0%
AACAATCAGATGCTAATTTC+TGG + chr4.2:44594257-44594276 None:intergenic 30.0%
AATCAGGAAAAAGGTTAATC+AGG - chr4.2:44594880-44594899 MS.gene08700:CDS 30.0%
CTGAACACAAATCATGATAA+TGG - chr4.2:44593376-44593395 MS.gene08700:intron 30.0%
GAGAAATTGTTCACAAGATT+TGG + chr4.2:44592666-44592685 None:intergenic 30.0%
TGCTTTCAAAAACTGAAACT+GGG + chr4.2:44594406-44594425 None:intergenic 30.0%
TTGCAACATAAAGTCTATGT+AGG - chr4.2:44593818-44593837 MS.gene08700:intron 30.0%
! ATAGGGTTTATCCTTTTCAA+CGG - chr4.2:44592898-44592917 MS.gene08700:CDS 30.0%
! TAATTTTACTCGCATGCTTT+TGG - chr4.2:44593886-44593905 MS.gene08700:intron 30.0%
!! TTTTGAAAAGGAGGTATGAA+TGG + chr4.2:44594539-44594558 None:intergenic 30.0%
AAAGTTGTAATTCTCAACCG+AGG + chr4.2:44594438-44594457 None:intergenic 35.0%
AAATTCTCACAAGAAGGTCT+TGG - chr4.2:44594856-44594875 MS.gene08700:CDS 35.0%
AACTCAATCAACCTAGTCAT+TGG + chr4.2:44593800-44593819 None:intergenic 35.0%
AATTATCAGATGCCATCCTA+CGG + chr4.2:44594083-44594102 None:intergenic 35.0%
ATAGTACTCATACCTTAACG+TGG - chr4.2:44593560-44593579 MS.gene08700:intron 35.0%
CAAAGGCTAATTCGTTTGAT+AGG - chr4.2:44592880-44592899 MS.gene08700:CDS 35.0%
CACAGAGAAAATCACTTGAA+AGG + chr4.2:44594128-44594147 None:intergenic 35.0%
CTGCTTTCAAAAACTGAAAC+TGG + chr4.2:44594407-44594426 None:intergenic 35.0%
GCGTGTATACATGTAAAGTA+TGG - chr4.2:44594155-44594174 MS.gene08700:intron 35.0%
GGTAATGAATGACATTCCTT+TGG - chr4.2:44593709-44593728 MS.gene08700:intron 35.0%
GTTAATCAGGAGACTGTTAA+AGG - chr4.2:44594893-44594912 MS.gene08700:CDS 35.0%
TAGAAAATGTACGCATTGTG+AGG - chr4.2:44594691-44594710 MS.gene08700:intron 35.0%
TATCACTGGATTACAAAGCT+AGG - chr4.2:44594040-44594059 MS.gene08700:intron 35.0%
TGAAAGCAGTAGTTATTCCT+CGG - chr4.2:44594418-44594437 MS.gene08700:intron 35.0%
TTACTACAATGATGTTGTGC+AGG - chr4.2:44593022-44593041 MS.gene08700:CDS 35.0%
TTCGATGGAACAAATTTACG+CGG - chr4.2:44594365-44594384 MS.gene08700:intron 35.0%
! TAGGGTTTATCCTTTTCAAC+GGG - chr4.2:44592899-44592918 MS.gene08700:CDS 35.0%
! TATGCTTGCTTTGATGACTA+TGG - chr4.2:44592962-44592981 MS.gene08700:CDS 35.0%
! TCCTTTTCAAAAGCAGCAAT+CGG - chr4.2:44594547-44594566 MS.gene08700:intron 35.0%
! TGATGACTATGGCATTTTGT+TGG - chr4.2:44592973-44592992 MS.gene08700:CDS 35.0%
! TGATTTTCCACTTGAAGATG+TGG - chr4.2:44592524-44592543 MS.gene08700:CDS 35.0%
!! CAAGAATTTTAACGGTGTGT+CGG - chr4.2:44592470-44592489 MS.gene08700:CDS 35.0%
!! CTTTCAAGTGATTTTCTCTG+TGG - chr4.2:44594126-44594145 MS.gene08700:intron 35.0%
!! TTGTTGGTCAGGATTTTTAG+CGG - chr4.2:44592989-44593008 MS.gene08700:CDS 35.0%
AAAAACTGAAACTGGGCTGT+AGG + chr4.2:44594399-44594418 None:intergenic 40.0%
ACAAGAAGGTCTTGGAAATC+AGG - chr4.2:44594864-44594883 MS.gene08700:CDS 40.0%
ACTGAGAAAACACCAAGAGA+TGG + chr4.2:44592603-44592622 None:intergenic 40.0%
AGAGTCCTGTTGTGTATGTT+TGG - chr4.2:44592850-44592869 MS.gene08700:CDS 40.0%
AGCAACCAAACATACACAAC+AGG + chr4.2:44592858-44592877 None:intergenic 40.0%
ATCATCGATCGATCTTATCC+TGG + chr4.2:44593742-44593761 None:intergenic 40.0%
ATCCAAAATGTCTCCTTCGA+TGG - chr4.2:44594350-44594369 MS.gene08700:intron 40.0%
ATTATCAGATGCCATCCTAC+GGG + chr4.2:44594082-44594101 None:intergenic 40.0%
CATTATGCAGAGCCCAAAAA+AGG - chr4.2:44594591-44594610 MS.gene08700:intron 40.0%
CTCAATGCTATTAGAGAGCT+GGG + chr4.2:44594636-44594655 None:intergenic 40.0%
CTCAGAAAGTGAGAAAGTTC+TGG - chr4.2:44593595-44593614 MS.gene08700:intron 40.0%
CTTGAAGATGGTTTATGGCT+AGG - chr4.2:44593680-44593699 MS.gene08700:intron 40.0%
CTTGAAGATGTGGAAACTGA+TGG - chr4.2:44592534-44592553 MS.gene08700:CDS 40.0%
GAAAGTTACACCCGTTGAAA+AGG + chr4.2:44592912-44592931 None:intergenic 40.0%
GACCATCATACAGTCTTCAA+CGG - chr4.2:44594004-44594023 MS.gene08700:intron 40.0%
GCACTCAAATTCTCACAAGA+AGG - chr4.2:44594850-44594869 MS.gene08700:CDS 40.0%
GGTCTTGGAAATCAGGAAAA+AGG - chr4.2:44594871-44594890 MS.gene08700:CDS 40.0%
GTATGTTTGGTTGCTTGCAA+AGG - chr4.2:44592863-44592882 MS.gene08700:CDS 40.0%
GTCGCCTATTTCCTGAATAT+CGG - chr4.2:44594795-44594814 MS.gene08700:CDS 40.0%
GTGATTGAACTCTGTATCAC+TGG - chr4.2:44594026-44594045 MS.gene08700:intron 40.0%
GTGTGCTTGAAGATGGTTTA+TGG - chr4.2:44593675-44593694 MS.gene08700:intron 40.0%
TACTCATACCTTAACGTGGA+GGG - chr4.2:44593564-44593583 MS.gene08700:intron 40.0%
TATATGTTTAGAGACGGGAG+AGG - chr4.2:44593976-44593995 MS.gene08700:intron 40.0%
TCAAGCTTAAAAGATCGACG+AGG - chr4.2:44593080-44593099 MS.gene08700:CDS 40.0%
TCAATCAACCTAGTCATTGG+CGG + chr4.2:44593797-44593816 None:intergenic 40.0%
TCAGTTTCCACATCTTCAAG+TGG + chr4.2:44592534-44592553 None:intergenic 40.0%
TCTCAATGCTATTAGAGAGC+TGG + chr4.2:44594637-44594656 None:intergenic 40.0%
TGGGAGATATTGTCTTTCCT+TGG + chr4.2:44594617-44594636 None:intergenic 40.0%
TGGTTTATGGCTAGGTAACA+TGG - chr4.2:44593688-44593707 MS.gene08700:intron 40.0%
TTCATGTGTGTGCTTGAAGA+TGG - chr4.2:44593668-44593687 MS.gene08700:intron 40.0%
! AACTCCACAAGCATTGCATA+GGG + chr4.2:44594762-44594781 None:intergenic 40.0%
! ACGAGGACTAGTAAGTGAAA+GGG - chr4.2:44593097-44593116 MS.gene08700:intron 40.0%
! ACTATGGCATTTTGTTGGTC+AGG - chr4.2:44592978-44592997 MS.gene08700:CDS 40.0%
! ATCGAAGGAGACATTTTGGA+TGG + chr4.2:44594351-44594370 None:intergenic 40.0%
! GCGTAAATTTGTTCCATCGA+AGG + chr4.2:44594366-44594385 None:intergenic 40.0%
! TCGAAGGAGACATTTTGGAT+GGG + chr4.2:44594350-44594369 None:intergenic 40.0%
! TTCCATCGAAGGAGACATTT+TGG + chr4.2:44594355-44594374 None:intergenic 40.0%
!! GGAATAGTTTTGCCATATGC+AGG + chr4.2:44594329-44594348 None:intergenic 40.0%
!!! GATTGCTGCTTTTGAAAAGG+AGG + chr4.2:44594548-44594567 None:intergenic 40.0%
!!! GTCTTTCCTTGGCCTTTTTT+GGG + chr4.2:44594606-44594625 None:intergenic 40.0%
!!! TCCGATTGCTGCTTTTGAAA+AGG + chr4.2:44594551-44594570 None:intergenic 40.0%
!!! TCTTGGTGTTTTCTCAGTAC+CGG - chr4.2:44592605-44592624 MS.gene08700:CDS 40.0%
!!! TGTCTTTCCTTGGCCTTTTT+TGG + chr4.2:44594607-44594626 None:intergenic 40.0%
!!! TGTTGGTCAGGATTTTTAGC+GGG - chr4.2:44592990-44593009 MS.gene08700:CDS 40.0%
AAACAGCTTGGAAAGACTGC+TGG - chr4.2:44594302-44594321 MS.gene08700:CDS 45.0%
AAGACCCTATGCAATGCTTG+TGG - chr4.2:44594755-44594774 MS.gene08700:CDS 45.0%
ACTCATACCTTAACGTGGAG+GGG - chr4.2:44593565-44593584 MS.gene08700:intron 45.0%
AGGTAACTGAAACGCCACAA+TGG - chr4.2:44594711-44594730 MS.gene08700:intron 45.0%
ATGCTTGTGGAGTTCGATAC+AGG - chr4.2:44594768-44594787 MS.gene08700:CDS 45.0%
CACCAAGAGATGGTTCTTCA+AGG + chr4.2:44592593-44592612 None:intergenic 45.0%
CACCGTTGAAGACTGTATGA+TGG + chr4.2:44594009-44594028 None:intergenic 45.0%
CACCTTGAAGAACCATCTCT+TGG - chr4.2:44592588-44592607 MS.gene08700:CDS 45.0%
CAGGCCGATATTCAGGAAAT+AGG + chr4.2:44594802-44594821 None:intergenic 45.0%
GTACTCATACCTTAACGTGG+AGG - chr4.2:44593563-44593582 MS.gene08700:intron 45.0%
TCCATCCAAACAGCAAAGAC+AGG + chr4.2:44592710-44592729 None:intergenic 45.0%
TTATCCTGGTTGTCAGCCAA+AGG + chr4.2:44593728-44593747 None:intergenic 45.0%
! AGATGTGGAAACTGATGGTG+TGG - chr4.2:44592539-44592558 MS.gene08700:CDS 45.0%
! CAAGCATTGCATAGGGTCTT+AGG + chr4.2:44594755-44594774 None:intergenic 45.0%
! CGAGGACTAGTAAGTGAAAG+GGG - chr4.2:44593098-44593117 MS.gene08700:intron 45.0%
! GAACTCCACAAGCATTGCAT+AGG + chr4.2:44594763-44594782 None:intergenic 45.0%
! GACGAGGACTAGTAAGTGAA+AGG - chr4.2:44593096-44593115 MS.gene08700:intron 45.0%
! GCACTGATGCTTCAAAGGTT+GGG + chr4.2:44594832-44594851 None:intergenic 45.0%
! TGAGTGCACTGATGCTTCAA+AGG + chr4.2:44594837-44594856 None:intergenic 45.0%
! TGCACTGATGCTTCAAAGGT+TGG + chr4.2:44594833-44594852 None:intergenic 45.0%
!! GTGATTTTCTCTGTGGACTG+TGG - chr4.2:44594133-44594152 MS.gene08700:intron 45.0%
!! TCCTGTCTTTGCTGTTTGGA+TGG - chr4.2:44592706-44592725 MS.gene08700:CDS 45.0%
!!! GATAATATTTTTTTAAAATA+AGG - chr4.2:44593938-44593957 MS.gene08700:intron 5.0%
AAACGCCACAATGGAGAGAG+GGG - chr4.2:44594720-44594739 MS.gene08700:CDS 50.0%
AAGACTGCTGGACCTGCATA+TGG - chr4.2:44594314-44594333 MS.gene08700:CDS 50.0%
CAATCGGATAAACAGCGTGC+TGG - chr4.2:44594563-44594582 MS.gene08700:intron 50.0%
CATAGGGTCTTAGGACCCTT+AGG + chr4.2:44594746-44594765 None:intergenic 50.0%
CATTCCTTTGGCTGACAACC+AGG - chr4.2:44593721-44593740 MS.gene08700:intron 50.0%
CTGAAACTGGGCTGTAGGTT+CGG + chr4.2:44594394-44594413 None:intergenic 50.0%
GAAACGCCACAATGGAGAGA+GGG - chr4.2:44594719-44594738 MS.gene08700:CDS 50.0%
GGTGTGTCGGATGAGATTCT+TGG - chr4.2:44592483-44592502 MS.gene08700:CDS 50.0%
TGAAACGCCACAATGGAGAG+AGG - chr4.2:44594718-44594737 MS.gene08700:CDS 50.0%
TGTGGAGTTCGATACAGGTC+TGG - chr4.2:44594773-44594792 MS.gene08700:CDS 50.0%
TTACCACGAGGACGCTTTGT+CGG + chr4.2:44594476-44594495 None:intergenic 50.0%
! CTGATGGTGTGGAACAGGAT+TGG - chr4.2:44592550-44592569 MS.gene08700:CDS 50.0%
! GGAAACTGATGGTGTGGAAC+AGG - chr4.2:44592545-44592564 MS.gene08700:CDS 50.0%
!! CTGCTCCTGTCTTTGCTGTT+TGG - chr4.2:44592702-44592721 MS.gene08700:CDS 50.0%
ACAATGGAGAGAGGGGCCTA+AGG - chr4.2:44594727-44594746 MS.gene08700:CDS 55.0%
AGAGCCTGTCGAAGCTTGAG+AGG + chr4.2:44594508-44594527 None:intergenic 55.0%
CAATGGAGAGAGGGGCCTAA+GGG - chr4.2:44594728-44594747 MS.gene08700:CDS 55.0%
CGCAATACCCCTCCACGTTA+AGG + chr4.2:44593575-44593594 None:intergenic 55.0%
CTAGCTGCAGGCCGATATTC+AGG + chr4.2:44594809-44594828 None:intergenic 55.0%
CTGTCATGCCGCCAATGACT+AGG - chr4.2:44593786-44593805 MS.gene08700:intron 55.0%
GCAGAGCCCAAAAAAGGCCA+AGG - chr4.2:44594597-44594616 MS.gene08700:intron 55.0%
TACCACGAGGACGCTTTGTC+GGG + chr4.2:44594475-44594494 None:intergenic 55.0%
TTAGGCCCCTCTCTCCATTG+TGG + chr4.2:44594728-44594747 None:intergenic 55.0%
! GTACCGGCATTTGAGCTACG+CGG - chr4.2:44592621-44592640 MS.gene08700:CDS 55.0%
!! TCAAAGGTTGGGCTAGCTGC+AGG + chr4.2:44594821-44594840 None:intergenic 55.0%
AGCGTCCTCGTGGTAAACGC+CGG - chr4.2:44594480-44594499 MS.gene08700:intron 60.0%
ATTCGCCAAAGCGCACCCGT+AGG - chr4.2:44594064-44594083 MS.gene08700:intron 60.0%
GGACGCTTTGTCGGGATCAC+TGG + chr4.2:44594467-44594486 None:intergenic 60.0%
TCGCCGCGTAGCTCAAATGC+CGG + chr4.2:44592627-44592646 None:intergenic 60.0%
! ATCCCGACAAAGCGTCCTCG+TGG - chr4.2:44594470-44594489 MS.gene08700:intron 60.0%
! TGTCGAAGCTTGAGAGGCGC+CGG + chr4.2:44594502-44594521 None:intergenic 60.0%
GCCAAAGCGCACCCGTAGGA+TGG - chr4.2:44594068-44594087 MS.gene08700:intron 65.0%
GCCATCCTACGGGTGCGCTT+TGG + chr4.2:44594072-44594091 None:intergenic 65.0%
GGCGCCTCTCAAGCTTCGAC+AGG - chr4.2:44594501-44594520 MS.gene08700:intron 65.0%
!! AGGCGCCGGCGTTTACCACG+AGG + chr4.2:44594488-44594507 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 44592447 44594961 44592447 ID=MS.gene08700
chr4.2 mRNA 44592447 44594961 44592447 ID=MS.gene08700.t1;Parent=MS.gene08700
chr4.2 exon 44594716 44594961 44594716 ID=MS.gene08700.t1.exon1;Parent=MS.gene08700.t1
chr4.2 CDS 44594716 44594961 44594716 ID=cds.MS.gene08700.t1;Parent=MS.gene08700.t1
chr4.2 exon 44594302 44594364 44594302 ID=MS.gene08700.t1.exon2;Parent=MS.gene08700.t1
chr4.2 CDS 44594302 44594364 44594302 ID=cds.MS.gene08700.t1;Parent=MS.gene08700.t1
chr4.2 exon 44592447 44593100 44592447 ID=MS.gene08700.t1.exon3;Parent=MS.gene08700.t1
chr4.2 CDS 44592447 44593100 44592447 ID=cds.MS.gene08700.t1;Parent=MS.gene08700.t1
Gene Sequence

>MS.gene08700

ATGAAGAACAGCTGGTTTTTTGACAAGAATTTTAACGGTGTGTCGGATGAGATTCTTGGTGACGTCGTTGAGTTTTTTGATTTTCCACTTGAAGATGTGGAAACTGATGGTGTGGAACAGGATTGGAATGATCAATTCAAACACCTTGAAGAACCATCTCTTGGTGTTTTCTCAGTACCGGCATTTGAGCTACGCGGCGAAACTCAAGACGAGAAACCAAATCTTGTGAACAATTTCTCTGCTGCTGTGAGTTTTCTGCTCCTGTCTTTGCTGTTTGGATGGACGTATTTTTCTACTTAATAATTATATTAATTTGATAAATTAATTATGTGCATGGTTTGAATATGTGAAAAACTAAAATTACATAAATATATCAACATCGAACCACACATTTTTTATTATCAGAGTCCTGTTGTGTATGTTTGGTTGCTTGCAAAGGCTAATTCGTTTGATAGGGTTTATCCTTTTCAACGGGTGTAACTTTCTTCTTAGACGTTTTTAATTGGAGAAGTTTGTATGCTTGCTTTGATGACTATGGCATTTTGTTGGTCAGGATTTTTAGCGGGAATTGAGAATTACTACAATGATGTTGTGCAGGAACTTTCTCATATTGACAGAAGAAATGTTGTGAATTCAAGCTTAAAAGATCGACGAGGACTAGTAAGTGAAAGGGGAGAATGTAATGATTTTAATGTTTAAGTATCTTTTTGACTCGGTTCTTTTTGAAATGTTTTCTAGGCTGTTAGAGATAGATGTGTTATAAAACTTTTTTGGGGATAAGAGAGAAGATTTGTTGAATCACTTAAAGTCGAGTTTAGAAGAACATGAGCAAAATCAAATGCTAAAGTTTCAATTTTCATGCAATTAGTTGATAAACATGATTATAAAATATGCTGATGATATCTTTACTTTTATGATACATTTTGGTTCTGAACACAAATCATGATAATGGTAACACTTACTGAGTAAAAAACTAAAAGCTACGCTCAAAATATGTTCAAAAAAGGACTAACTAGTAATCACACAAATTGTTCGTGTATGATTAATTTTCAGGTATTAGTTTTACAATATGATACTTTGACACTGAAAATCTGTCTCAAATTCTCTTGGTAAATAGTACTCATACCTTAACGTGGAGGGGTATTGCGCTCAGAAAGTGAGAAAGTTCTGGTAACCATGTTTTGAAGTTGTAAGTATTTTAGTTGTGATGAATAGATGGTATTCATGTGTGTGCTTGAAGATGGTTTATGGCTAGGTAACATGGTAATGAATGACATTCCTTTGGCTGACAACCAGGATAAGATCGATCGATGATCACAAATGACAAAATAAGTAGAAACTGTCATGCCGCCAATGACTAGGTTGATTGAGTTGCAACATAAAGTCTATGTAGGCAAGTTTGTGCAGAATCACAAGCTGAAGTTGCAAAAAGATACAAGTAATTTTACTCGCATGCTTTTGGCTCAATATATGATTGATGTTACAGAAACTGATAATATTTTTTTAAAATAAGGGAGGGGAAAAATTATTATATGTTTAGAGACGGGAGAGGCATTTGACCATCATACAGTCTTCAACGGTGATTGAACTCTGTATCACTGGATTACAAAGCTAGGCATTCGCCAAAGCGCACCCGTAGGATGGCATCTGATAATTGAAAAGTATTTATTATAAATTTCCTTTCAAGTGATTTTCTCTGTGGACTGTGGCGTGTATACATGTAAAGTATGGAAGAATTTTAGTGGATTCCTTGAGTTTAATTAAAACATTATGTACTATTGAAAGTTTAATCTTTTCATTTCTGTTGCCAGAAATTAGCATCTGATTGTTTTATATTTTGCGTGTTTTTCAAATAAAACAGCTTGGAAAGACTGCTGGACCTGCATATGGCAAAACTATTCCCATCCAAAATGTCTCCTTCGATGGAACAAATTTACGCGGATGCCGAACCTACAGCCCAGTTTCAGTTTTTGAAAGCAGTAGTTATTCCTCGGTTGAGAATTACAACTTTGAATTACCAGTGATCCCGACAAAGCGTCCTCGTGGTAAACGCCGGCGCCTCTCAAGCTTCGACAGGCTCTTCTCAATTCCATTCATACCTCCTTTTCAAAAGCAGCAATCGGATAAACAGCGTGCTGGTAAATCATTATGCAGAGCCCAAAAAAGGCCAAGGAAAGACAATATCTCCCAGCTCTCTAATAGCATTGAGATGAAGAGATCATCGTTAAATGAATCAATTGCTCGTAGAAAATGTACGCATTGTGAGGTAACTGAAACGCCACAATGGAGAGAGGGGCCTAAGGGTCCTAAGACCCTATGCAATGCTTGTGGAGTTCGATACAGGTCTGGTCGCCTATTTCCTGAATATCGGCCTGCAGCTAGCCCAACCTTTGAAGCATCAGTGCACTCAAATTCTCACAAGAAGGTCTTGGAAATCAGGAAAAAGGTTAATCAGGAGACTGTTAAAGGTTCTTCCATGTTGTATTTATCATCAAACCTTTCAGGAATTCTCTAG
Protein sequence

>MS.gene08700.t1

MKNSWFFDKNFNGVSDEILGDVVEFFDFPLEDVETDGVEQDWNDQFKHLEEPSLGVFSVPAFELRGETQDEKPNLVNNFSAAELSHIDRRNVVNSSLKDRRGLLGKTAGPAYGKTIPIQNVSFDGTNLRGCRTYSPVSVFESSSYSSVENYNFELPVIPTKRPRGKRRRLSSFDRLFSIPFIPPFQKQQSDKQRAGKSLCRAQKRPRKDNISQLSNSIEMKRSSLNESIARRKCTHCEVTETPQWREGPKGPKTLCNACGVRYRSGRLFPEYRPAASPTFEASVHSNSHKKVLEIRKKVNQETVKGSSMLYLSSNLSGIL