Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08762.t1 | RHN42614.1 | 97.7 | 220 | 3 | 1 | 25 | 244 | 1 | 218 | 2.90E-122 | 448 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08762.t1 | Q6NLS8 | 72.9 | 218 | 56 | 2 | 25 | 241 | 1 | 216 | 2.3e-88 | 326.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08762.t1 | A0A396GSX3 | 97.7 | 220 | 3 | 1 | 25 | 244 | 1 | 218 | 2.1e-122 | 448.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene08762 | MS.gene73771 | PPI |
| MS.gene08762 | MS.gene024170 | PPI |
| MS.gene08762 | MS.gene55048 | PPI |
| MS.gene08762 | MS.gene033605 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08762.t1 | MTR_8g085910 | 97.273 | 220 | 4 | 1 | 25 | 244 | 1 | 218 | 3.97e-159 | 440 |
| MS.gene08762.t1 | MTR_7g082240 | 58.586 | 198 | 80 | 1 | 41 | 238 | 56 | 251 | 2.55e-82 | 246 |
| MS.gene08762.t1 | MTR_8g095500 | 57.576 | 198 | 82 | 1 | 41 | 238 | 48 | 243 | 4.40e-81 | 243 |
| MS.gene08762.t1 | MTR_8g103890 | 56.633 | 196 | 83 | 1 | 40 | 235 | 63 | 256 | 1.12e-78 | 238 |
| MS.gene08762.t1 | MTR_8g103890 | 66.176 | 136 | 44 | 1 | 40 | 175 | 63 | 196 | 5.49e-61 | 191 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08762.t1 | AT5G19830 | 72.936 | 218 | 56 | 2 | 25 | 241 | 1 | 216 | 1.13e-112 | 322 |
| MS.gene08762.t1 | AT1G18440 | 53.571 | 196 | 89 | 1 | 41 | 236 | 94 | 287 | 6.55e-77 | 234 |
| MS.gene08762.t1 | AT5G16140 | 57.071 | 198 | 83 | 1 | 41 | 238 | 45 | 240 | 7.09e-77 | 232 |
| MS.gene08762.t1 | AT5G16140 | 57.071 | 198 | 83 | 1 | 41 | 238 | 45 | 240 | 7.09e-77 | 232 |
| MS.gene08762.t1 | AT5G38290 | 56.566 | 198 | 84 | 1 | 41 | 238 | 55 | 250 | 5.21e-75 | 228 |
| MS.gene08762.t1 | AT5G38290 | 56.566 | 198 | 84 | 1 | 41 | 238 | 51 | 246 | 1.06e-74 | 227 |
| MS.gene08762.t1 | AT1G18440 | 64.486 | 107 | 36 | 1 | 41 | 147 | 94 | 198 | 8.08e-46 | 152 |
Find 63 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGCTTCTTCTATTTAA+AGG | 0.160898 | 4.2:+12650331 | MS.gene08762:CDS |
| TTACCACAAGCACCTGATTA+AGG | 0.270638 | 4.2:-12645843 | None:intergenic |
| GTGCTTCGTCTTAATGAAAA+AGG | 0.284744 | 4.2:+12645986 | MS.gene08762:CDS |
| CAGTCACGGTTTCATTGCTT+CGG | 0.304111 | 4.2:-12643650 | None:intergenic |
| TTACAAGTTACCCCTTAATC+AGG | 0.359613 | 4.2:+12645831 | MS.gene08762:CDS |
| GCAAATCACCTTACAAATAT+CGG | 0.365826 | 4.2:-12643196 | None:intergenic |
| CGGTTTCATTGCTTCGGGTT+TGG | 0.370142 | 4.2:-12643644 | None:intergenic |
| GCTTCTTCTATTTAAAGGTT+TGG | 0.376967 | 4.2:+12650336 | MS.gene08762:CDS |
| GAATTTCCTCGTCTAAGAAT+TGG | 0.379292 | 4.2:+12649704 | MS.gene08762:CDS |
| TTTAAATAGAAGAAGCTTCA+AGG | 0.387124 | 4.2:-12650329 | None:intergenic |
| GTTTGGAAGTGAGTGCAAAA+AGG | 0.388013 | 4.2:+12650353 | MS.gene08762:CDS |
| AGCATATCCCCGCCGCGTTT+AGG | 0.402185 | 4.2:-12643678 | None:intergenic |
| TGAGGCTTTGCAAGGAAAAC+GGG | 0.410154 | 4.2:-12644076 | None:intergenic |
| CTGAGATTCATATAAGTTTG+AGG | 0.413922 | 4.2:-12644094 | None:intergenic |
| ATATAATGGTGTACCCTTTG+TGG | 0.418031 | 4.2:-12646021 | None:intergenic |
| GCACCGTCGCTCCACGCCCT+TGG | 0.421493 | 4.2:+12643727 | MS.gene08762:CDS |
| TTGAGGCTTTGCAAGGAAAA+CGG | 0.428515 | 4.2:-12644077 | None:intergenic |
| GTTCCTTTGTATTTGTCGCC+AGG | 0.436315 | 4.2:-12643771 | None:intergenic |
| TGGGAAGCCTCCTGGACAAA+TGG | 0.438531 | 4.2:+12649822 | MS.gene08762:CDS |
| TACCACAAGCACCTGATTAA+GGG | 0.444523 | 4.2:-12645842 | None:intergenic |
| AAGTGCAGACATTACCAGTT+TGG | 0.444811 | 4.2:+12650407 | MS.gene08762:CDS |
| AGTCACGGTTTCATTGCTTC+GGG | 0.462712 | 4.2:-12643649 | None:intergenic |
| ACAGGGATTGGGAAGCCTCC+TGG | 0.466135 | 4.2:+12649814 | MS.gene08762:intron |
| ATAATCGAATCCTACCAAAC+TGG | 0.489126 | 4.2:-12650421 | None:intergenic |
| CTGAACCACCTAAACGCGGC+GGG | 0.497390 | 4.2:+12643670 | MS.gene08762:CDS |
| GCTTTAGGATCCATTTGTCC+AGG | 0.507125 | 4.2:-12649832 | None:intergenic |
| TTCGTTGGATTGGGTAACCC+TGG | 0.517492 | 4.2:+12643753 | MS.gene08762:CDS |
| TTTACTGTAGGCAGGACCAC+TGG | 0.521794 | 4.2:+12645801 | MS.gene08762:intron |
| AAGCCAAGGGCGTGGAGCGA+CGG | 0.525217 | 4.2:-12643730 | None:intergenic |
| GCAGACATTACCAGTTTGGT+AGG | 0.529698 | 4.2:+12650411 | MS.gene08762:CDS |
| TTCCTTTGTATTTGTCGCCA+GGG | 0.533567 | 4.2:-12643770 | None:intergenic |
| TATAAGTTTGAGGCTTTGCA+AGG | 0.536193 | 4.2:-12644084 | None:intergenic |
| GACGAAGCACCCCACATGGC+AGG | 0.549108 | 4.2:-12645973 | None:intergenic |
| ACCCCTTAATCAGGTGCTTG+TGG | 0.558666 | 4.2:+12645840 | MS.gene08762:CDS |
| CCCAATCCAACGAAAAGCCA+AGG | 0.560848 | 4.2:-12643744 | None:intergenic |
| CCAACGAAAAGCCAAGGGCG+TGG | 0.564814 | 4.2:-12643738 | None:intergenic |
| GCGTTTAGGTGGTTCAGTCA+CGG | 0.568836 | 4.2:-12643664 | None:intergenic |
| ATATCCCCGCCGCGTTTAGG+TGG | 0.576009 | 4.2:-12643675 | None:intergenic |
| GGGGTCATGGAAGCCACAAA+GGG | 0.578213 | 4.2:+12646008 | MS.gene08762:CDS |
| GATGCATTTGCTGAGTCACA+AGG | 0.578919 | 4.2:+12643921 | MS.gene08762:CDS |
| TTAGGATCCATTTGTCCAGG+AGG | 0.588942 | 4.2:-12649829 | None:intergenic |
| ATGATATGAACCTGCCATGT+GGG | 0.592556 | 4.2:+12645963 | MS.gene08762:CDS |
| TTGACGAGGCTTTGCACGAG+GGG | 0.595008 | 4.2:+12650299 | MS.gene08762:CDS |
| TTTGCAATGAACTGTATCCA+TGG | 0.598839 | 4.2:-12643949 | None:intergenic |
| ACCACAAGCACCTGATTAAG+GGG | 0.601653 | 4.2:-12645841 | None:intergenic |
| ATGGCAGGTTCATATCATCA+TGG | 0.602883 | 4.2:-12645958 | None:intergenic |
| GATTGACGAGGCTTTGCACG+AGG | 0.603038 | 4.2:+12650297 | MS.gene08762:intron |
| TGAACCACCTAAACGCGGCG+GGG | 0.646060 | 4.2:+12643671 | MS.gene08762:CDS |
| TTAAGACGAAGCACCCCACA+TGG | 0.647130 | 4.2:-12645977 | None:intergenic |
| ATGCATTTGCTGAGTCACAA+GGG | 0.647645 | 4.2:+12643922 | MS.gene08762:CDS |
| TGACGAGGCTTTGCACGAGG+GGG | 0.650076 | 4.2:+12650300 | MS.gene08762:CDS |
| TACTAACCAATTCTTAGACG+AGG | 0.656829 | 4.2:-12649710 | None:intergenic |
| TCTTGTTGGACAGATTGACG+AGG | 0.661063 | 4.2:+12650285 | MS.gene08762:intron |
| CCAATCCAACGAAAAGCCAA+GGG | 0.661283 | 4.2:-12643743 | None:intergenic |
| AACCCTGGCGACAAATACAA+AGG | 0.668364 | 4.2:+12643768 | MS.gene08762:CDS |
| AACTTGTAATAAGCAGCCAG+TGG | 0.676299 | 4.2:-12645817 | None:intergenic |
| ATTGACGAGGCTTTGCACGA+GGG | 0.680803 | 4.2:+12650298 | MS.gene08762:CDS |
| TGAGTCACAAGGGATTGCCA+TGG | 0.696153 | 4.2:+12643932 | MS.gene08762:CDS |
| ACTGAACCACCTAAACGCGG+CGG | 0.714344 | 4.2:+12643669 | MS.gene08762:CDS |
| TGATATGAACCTGCCATGTG+GGG | 0.716207 | 4.2:+12645964 | MS.gene08762:CDS |
| CAAACTTATATGAATCTCAG+TGG | 0.724547 | 4.2:+12644097 | MS.gene08762:CDS |
| GTGACTGAACCACCTAAACG+CGG | 0.726024 | 4.2:+12643666 | MS.gene08762:CDS |
| GATGATATGAACCTGCCATG+TGG | 0.757788 | 4.2:+12645962 | MS.gene08762:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 12643192 | 12650432 | 12643192 | ID=MS.gene08762 |
| chr4.2 | mRNA | 12643192 | 12650432 | 12643192 | ID=MS.gene08762.t1;Parent=MS.gene08762 |
| chr4.2 | exon | 12643192 | 12643204 | 12643192 | ID=MS.gene08762.t1.exon1;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12643192 | 12643204 | 12643192 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12643637 | 12643809 | 12643637 | ID=MS.gene08762.t1.exon2;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12643637 | 12643809 | 12643637 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12643903 | 12643987 | 12643903 | ID=MS.gene08762.t1.exon3;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12643903 | 12643987 | 12643903 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12644059 | 12644126 | 12644059 | ID=MS.gene08762.t1.exon4;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12644059 | 12644126 | 12644059 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12645811 | 12645861 | 12645811 | ID=MS.gene08762.t1.exon5;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12645811 | 12645861 | 12645811 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12645956 | 12646029 | 12645956 | ID=MS.gene08762.t1.exon6;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12645956 | 12646029 | 12645956 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12649667 | 12649725 | 12649667 | ID=MS.gene08762.t1.exon7;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12649667 | 12649725 | 12649667 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12649818 | 12649894 | 12649818 | ID=MS.gene08762.t1.exon8;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12649818 | 12649894 | 12649818 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| chr4.2 | exon | 12650298 | 12650432 | 12650298 | ID=MS.gene08762.t1.exon9;Parent=MS.gene08762.t1 |
| chr4.2 | CDS | 12650298 | 12650432 | 12650298 | ID=cds.MS.gene08762.t1;Parent=MS.gene08762.t1 |
| Gene Sequence |
| Protein sequence |
