Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08829.t1 | XP_003629619.2 | 97.8 | 880 | 18 | 1 | 1 | 880 | 1 | 879 | 0.00E+00 | 1692.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08829.t1 | O81852 | 79.0 | 829 | 167 | 3 | 56 | 880 | 54 | 879 | 0.0e+00 | 1316.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08829.t1 | G7LH36 | 97.8 | 880 | 18 | 1 | 1 | 880 | 1 | 879 | 0.0e+00 | 1692.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049297 | MS.gene08829 | 0.817925 | 2.56E-52 | -1.69E-46 |
| MS.gene051663 | MS.gene08829 | 0.809745 | 1.63E-50 | -1.69E-46 |
| MS.gene052254 | MS.gene08829 | 0.808953 | 2.40E-50 | -1.69E-46 |
| MS.gene052256 | MS.gene08829 | 0.830841 | 2.34E-55 | -1.69E-46 |
| MS.gene052665 | MS.gene08829 | 0.806713 | 7.20E-50 | -1.69E-46 |
| MS.gene052789 | MS.gene08829 | 0.81672 | 4.78E-52 | -1.69E-46 |
| MS.gene054942 | MS.gene08829 | 0.825927 | 3.59E-54 | -1.69E-46 |
| MS.gene057054 | MS.gene08829 | 0.82487 | 6.39E-54 | -1.69E-46 |
| MS.gene058833 | MS.gene08829 | 0.828108 | 1.08E-54 | -1.69E-46 |
| MS.gene058982 | MS.gene08829 | 0.818838 | 1.59E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08829.t1 | MTR_8g083210 | 97.841 | 880 | 18 | 1 | 1 | 880 | 1 | 879 | 0.0 | 1779 |
| MS.gene08829.t1 | MTR_8g083210 | 97.045 | 880 | 18 | 2 | 1 | 880 | 1 | 872 | 0.0 | 1760 |
| MS.gene08829.t1 | MTR_3g084500 | 82.249 | 845 | 146 | 1 | 36 | 880 | 29 | 869 | 0.0 | 1448 |
| MS.gene08829.t1 | MTR_3g084500 | 86.006 | 686 | 96 | 0 | 195 | 880 | 14 | 699 | 0.0 | 1236 |
| MS.gene08829.t1 | MTR_3g084500 | 86.595 | 649 | 87 | 0 | 232 | 880 | 1 | 649 | 0.0 | 1176 |
| MS.gene08829.t1 | MTR_7g083920 | 25.047 | 531 | 375 | 11 | 33 | 555 | 21 | 536 | 1.78e-48 | 181 |
| MS.gene08829.t1 | MTR_6g033260 | 26.483 | 472 | 321 | 10 | 90 | 553 | 83 | 536 | 1.61e-45 | 173 |
| MS.gene08829.t1 | MTR_6g033260 | 26.708 | 483 | 308 | 11 | 90 | 553 | 83 | 538 | 9.30e-45 | 171 |
| MS.gene08829.t1 | MTR_3g079330 | 34.561 | 353 | 193 | 12 | 558 | 883 | 3 | 344 | 2.69e-42 | 159 |
| MS.gene08829.t1 | MTR_6g033260 | 27.692 | 455 | 289 | 13 | 90 | 529 | 83 | 512 | 5.59e-40 | 155 |
| MS.gene08829.t1 | MTR_7g083920 | 25.765 | 392 | 274 | 9 | 169 | 555 | 50 | 429 | 1.99e-39 | 152 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08829.t1 | AT4G19710 | 77.896 | 846 | 180 | 3 | 39 | 880 | 37 | 879 | 0.0 | 1369 |
| MS.gene08829.t1 | AT1G31230 | 77.212 | 825 | 186 | 1 | 56 | 880 | 52 | 874 | 0.0 | 1330 |
| MS.gene08829.t1 | AT4G19710 | 77.584 | 803 | 173 | 3 | 39 | 837 | 37 | 836 | 0.0 | 1291 |
| MS.gene08829.t1 | AT5G14060 | 27.766 | 479 | 308 | 13 | 90 | 553 | 85 | 540 | 1.03e-47 | 179 |
| MS.gene08829.t1 | AT5G14060 | 27.766 | 479 | 308 | 13 | 90 | 553 | 85 | 540 | 1.03e-47 | 179 |
| MS.gene08829.t1 | AT3G02020 | 27.677 | 495 | 324 | 13 | 90 | 572 | 86 | 558 | 5.25e-47 | 177 |
| MS.gene08829.t1 | AT5G13280 | 26.779 | 534 | 342 | 17 | 44 | 555 | 38 | 544 | 2.27e-45 | 172 |
| MS.gene08829.t1 | AT5G21060 | 33.623 | 345 | 190 | 11 | 563 | 879 | 8 | 341 | 3.64e-43 | 162 |
| MS.gene08829.t1 | AT5G21060 | 34.402 | 343 | 192 | 10 | 563 | 879 | 8 | 343 | 4.08e-43 | 161 |
| MS.gene08829.t1 | AT5G21060 | 34.402 | 343 | 192 | 10 | 563 | 879 | 8 | 343 | 4.75e-43 | 161 |
| MS.gene08829.t1 | AT5G14060 | 28.457 | 376 | 240 | 9 | 90 | 455 | 85 | 441 | 1.46e-38 | 150 |
Find 220 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCGAATTTGCCTTCTTATT+TGG | 0.069267 | 4.2:-13391879 | None:intergenic |
| GAAAATATTCCTTATCAAAA+TGG | 0.145381 | 4.2:-13384094 | None:intergenic |
| CCGAATTTGCCTTCTTATTT+GGG | 0.162811 | 4.2:-13391878 | None:intergenic |
| TCTTGGTCTGTTCACAAATT+TGG | 0.173756 | 4.2:+13382241 | MS.gene08829:CDS |
| TCAAGTTGATCCCGACTATT+TGG | 0.182843 | 4.2:+13383491 | MS.gene08829:CDS |
| TATCCCAAATTGTAGCATTT+TGG | 0.191249 | 4.2:+13386716 | MS.gene08829:CDS |
| ATTATTGGACCAGGGTTAAT+TGG | 0.198805 | 4.2:+13387063 | MS.gene08829:CDS |
| GCAAACTCCGGGTCAAATTT+TGG | 0.204913 | 4.2:-13393640 | None:intergenic |
| CCCAAATAAGAAGGCAAATT+CGG | 0.231744 | 4.2:+13391878 | MS.gene08829:CDS |
| TGATTTCTCAGCAGCAATTA+TGG | 0.234619 | 4.2:+13383632 | MS.gene08829:CDS |
| GGGATATGCGCTTCACTTAC+AGG | 0.236235 | 4.2:+13382072 | MS.gene08829:CDS |
| ATCTTCCTTGGATGCTGTTT+TGG | 0.244571 | 4.2:+13382426 | MS.gene08829:CDS |
| AAGAGGAGAAGCGGCTAATT+TGG | 0.249739 | 4.2:+13391728 | MS.gene08829:CDS |
| ACCATAGCAATGGGAATTAT+TGG | 0.255782 | 4.2:+13387048 | MS.gene08829:CDS |
| GCAGCCAAAATGCTACAATT+TGG | 0.265473 | 4.2:-13386720 | None:intergenic |
| TGCAAATTGAAGGCATATTC+AGG | 0.275578 | 4.2:+13392487 | MS.gene08829:CDS |
| TTTCGTCAAGCTTTGGATAA+TGG | 0.279878 | 4.2:+13384711 | MS.gene08829:CDS |
| TTGCGTGTAATGGGCATAAT+TGG | 0.289405 | 4.2:+13390817 | MS.gene08829:CDS |
| TCCTTTACAGCATATAAATA+AGG | 0.291552 | 4.2:+13395484 | MS.gene08829:intron |
| TTGTAGGACATGGGGAATTA+TGG | 0.292050 | 4.2:+13383280 | MS.gene08829:CDS |
| CATGTTGTACAGTCTTATTT+TGG | 0.293994 | 4.2:+13384018 | MS.gene08829:intron |
| ACTGGAATGGCTGGTGTTCC+AGG | 0.296123 | 4.2:+13384405 | MS.gene08829:CDS |
| AGATGTTGTCTCTAGTTATT+AGG | 0.297251 | 4.2:+13383310 | MS.gene08829:CDS |
| GAGCTCGTCAGGTCACAATT+TGG | 0.297523 | 4.2:+13383667 | MS.gene08829:CDS |
| GTTCTTACCCGTAGTGGTTC+TGG | 0.302693 | 4.2:-13393495 | None:intergenic |
| TTCGTCAAGCTTTGGATAAT+GGG | 0.308314 | 4.2:+13384712 | MS.gene08829:CDS |
| GGCTGCTGTTGGCCAGAAAA+TGG | 0.310860 | 4.2:+13386737 | MS.gene08829:CDS |
| CAAATTTGGTGGAACTTGTA+TGG | 0.317266 | 4.2:+13382255 | MS.gene08829:CDS |
| AACATTGATTTGCGTGTAAT+GGG | 0.323429 | 4.2:+13390808 | MS.gene08829:CDS |
| TTGCGTGGCCTCCTTGAAAC+TGG | 0.331214 | 4.2:+13392453 | MS.gene08829:CDS |
| TTCTCTCTATCCAGTATTTG+AGG | 0.338111 | 4.2:+13392349 | MS.gene08829:intron |
| CATCCATTTGCACAATTGTC+GGG | 0.343333 | 4.2:+13394357 | MS.gene08829:CDS |
| ATCAAGGTTTCGTCAAGCTT+TGG | 0.350380 | 4.2:+13384704 | MS.gene08829:CDS |
| TAACATTGATTTGCGTGTAA+TGG | 0.351437 | 4.2:+13390807 | MS.gene08829:CDS |
| TGATGATTCCGAGAGGAAAT+TGG | 0.352311 | 4.2:+13382321 | MS.gene08829:CDS |
| TTTATTTACTGCAGATGTTT+CGG | 0.364466 | 4.2:+13382177 | MS.gene08829:intron |
| GGTTGTTTCTGCAATGTCTA+AGG | 0.365895 | 4.2:+13382345 | MS.gene08829:CDS |
| ATAATTCTCGCAAGGGAATC+TGG | 0.369464 | 4.2:+13393429 | MS.gene08829:CDS |
| GAGAGTGGAAACGGCGGGAG+AGG | 0.373781 | 4.2:-13381732 | None:intergenic |
| GGATAATGGGCGTCTTTCTC+AGG | 0.374064 | 4.2:+13384725 | MS.gene08829:CDS |
| GATTTCTCAGCAGCAATTAT+GGG | 0.379368 | 4.2:+13383633 | MS.gene08829:CDS |
| CCAAATAAGAAGGCAAATTC+GGG | 0.385017 | 4.2:+13391879 | MS.gene08829:CDS |
| CAACCTTAAGGCCATGCTTC+GGG | 0.385314 | 4.2:+13382531 | MS.gene08829:CDS |
| AGGATAGTATAAAAGCTTTA+CGG | 0.387954 | 4.2:+13386992 | MS.gene08829:CDS |
| ATAATTCCTGTGATGAGATA+TGG | 0.389266 | 4.2:+13384066 | MS.gene08829:CDS |
| TGCAAGGTAATTATTGCTAC+TGG | 0.393938 | 4.2:+13383555 | MS.gene08829:CDS |
| CGAAGAGAGTCACCGAGCTC+TGG | 0.396604 | 4.2:+13382051 | MS.gene08829:CDS |
| AAAATATTCCTTATCAAAAT+GGG | 0.405457 | 4.2:-13384093 | None:intergenic |
| GGATAGTATAAAAGCTTTAC+GGG | 0.405815 | 4.2:+13386993 | MS.gene08829:CDS |
| TTGCTGGCCATTACTATGAT+TGG | 0.406119 | 4.2:+13391829 | MS.gene08829:CDS |
| TTCTCTATTTAGAGCTCGTC+AGG | 0.407974 | 4.2:+13383656 | MS.gene08829:CDS |
| GAGAAGTGAGAGAGGGATGA+AGG | 0.410014 | 4.2:-13381631 | None:intergenic |
| CAGCCAAAATGCTACAATTT+GGG | 0.410503 | 4.2:-13386719 | None:intergenic |
| GTGATTGAGCCTTATTGATA+AGG | 0.412222 | 4.2:-13382387 | None:intergenic |
| ACGGCTGATTCTGTCATTGC+TGG | 0.413603 | 4.2:+13391813 | MS.gene08829:CDS |
| GATGTTCCTCTCGAGTTGTT+TGG | 0.414825 | 4.2:+13394562 | MS.gene08829:CDS |
| CCTTAGCCAATGCGTTGAAA+AGG | 0.415646 | 4.2:-13386785 | None:intergenic |
| TTGCCGAGGCATTGCAATCA+AGG | 0.416755 | 4.2:+13384688 | MS.gene08829:CDS |
| AGCAAGGTGGAAAGAACTTC+GGG | 0.419364 | 4.2:+13391701 | MS.gene08829:CDS |
| TTATTGGACCAGGGTTAATT+GGG | 0.421244 | 4.2:+13387064 | MS.gene08829:CDS |
| TCTTGTTTCTTTGCAAACTC+CGG | 0.422683 | 4.2:-13393652 | None:intergenic |
| GATGTATGACCTTATCAATA+AGG | 0.423672 | 4.2:+13382378 | MS.gene08829:CDS |
| ATCTTTAATAACTTTAAAGA+TGG | 0.425147 | 4.2:+13392854 | MS.gene08829:CDS |
| TATGGCACGGACATTTATAT+TGG | 0.429442 | 4.2:-13386923 | None:intergenic |
| TAATAACTAGAGACAACATC+TGG | 0.432602 | 4.2:-13383308 | None:intergenic |
| CAAGAAGATGCTGACAATGC+TGG | 0.432766 | 4.2:+13393670 | MS.gene08829:CDS |
| CAGTCTACTAATGCCGTGTT+TGG | 0.437063 | 4.2:-13391789 | None:intergenic |
| GACAGATCATCTCTTGGATC+AGG | 0.442379 | 4.2:-13392929 | None:intergenic |
| TTATCAAGAGGCTTGGGAAA+TGG | 0.442785 | 4.2:+13383925 | MS.gene08829:CDS |
| TTAATAACTTTAAAGATGGC+CGG | 0.444869 | 4.2:+13392858 | MS.gene08829:CDS |
| GGTTCTTGAGAGATAAAATC+TGG | 0.445612 | 4.2:-13387025 | None:intergenic |
| CAGAAAATGGCAAGTACTCC+TGG | 0.445741 | 4.2:+13386750 | MS.gene08829:CDS |
| CATTGTCTTATCAAGAGGCT+TGG | 0.446236 | 4.2:+13383918 | MS.gene08829:CDS |
| GTTGTTGAAGTTGTCGTGTT+GGG | 0.452931 | 4.2:-13381674 | None:intergenic |
| GCAACCTTAAGGCCATGCTT+CGG | 0.454040 | 4.2:+13382530 | MS.gene08829:CDS |
| TGAGTCTTATATATCTTCCT+TGG | 0.456104 | 4.2:+13382414 | MS.gene08829:CDS |
| AAATGCGAGAGAAGTGAGAG+AGG | 0.460064 | 4.2:-13381639 | None:intergenic |
| ATTGTCTTATCAAGAGGCTT+GGG | 0.461098 | 4.2:+13383919 | MS.gene08829:CDS |
| TTAGCTCTTGTAAATGTTGA+GGG | 0.461391 | 4.2:+13384222 | MS.gene08829:CDS |
| CCACGCAAAGTGCTGACTAT+AGG | 0.461650 | 4.2:-13392438 | None:intergenic |
| GTCTTGATTGTAAATCCTAC+TGG | 0.462767 | 4.2:+13383462 | MS.gene08829:CDS |
| AAGAAGATGCTGACAATGCT+GGG | 0.463024 | 4.2:+13393671 | MS.gene08829:CDS |
| TCTGGCACTAGACTTTCAAT+TGG | 0.463231 | 4.2:-13393477 | None:intergenic |
| TAGCCCGAAGCATGGCCTTA+AGG | 0.464163 | 4.2:-13382534 | None:intergenic |
| CGCTCGGATTCCAAATAGTC+GGG | 0.464640 | 4.2:-13383501 | None:intergenic |
| TGGAACAGATGTTGCTAGAA+AGG | 0.465723 | 4.2:+13392952 | MS.gene08829:CDS |
| TAACGTTATCATGATATCTC+AGG | 0.468606 | 4.2:+13384470 | MS.gene08829:CDS |
| TCTTCATAATCACTAACAAC+AGG | 0.470083 | 4.2:-13384150 | None:intergenic |
| CTTGTTTCTTTGCAAACTCC+GGG | 0.470800 | 4.2:-13393651 | None:intergenic |
| TGGTCTGTTCACAAATTTGG+TGG | 0.471894 | 4.2:+13382244 | MS.gene08829:CDS |
| TGAGGTAGTTGGTGAAGCAA+AGG | 0.474066 | 4.2:+13392889 | MS.gene08829:CDS |
| GCACTCTACTCGATCAACTA+AGG | 0.475698 | 4.2:+13387088 | MS.gene08829:CDS |
| TCATCCATTTGCACAATTGT+CGG | 0.476880 | 4.2:+13394356 | MS.gene08829:CDS |
| GAAGTTGTCGTGTTGGGAGT+TGG | 0.479644 | 4.2:-13381668 | None:intergenic |
| GTTCCAGACAGATCATCTCT+TGG | 0.480054 | 4.2:-13392935 | None:intergenic |
| GGTTGCAGAACTGGAATGGC+TGG | 0.480922 | 4.2:+13384396 | MS.gene08829:intron |
| GACCCCGACAATTGTGCAAA+TGG | 0.481521 | 4.2:-13394360 | None:intergenic |
| ACTGCACATGACATACTTGA+TGG | 0.481804 | 4.2:+13382463 | MS.gene08829:CDS |
| TTTGGTGCAGTAAAAGATGT+TGG | 0.491702 | 4.2:+13384444 | MS.gene08829:CDS |
| TGAAGACATCAGCAACCTTA+AGG | 0.493168 | 4.2:+13382519 | MS.gene08829:CDS |
| CCATCCCGCTTCAATGTAGT+AGG | 0.493981 | 4.2:-13383606 | None:intergenic |
| AAAATAGCGCTAGCTGTACC+TGG | 0.496987 | 4.2:-13384423 | None:intergenic |
| TGTAATTACCCCAAATAAGA+AGG | 0.498233 | 4.2:+13391869 | MS.gene08829:CDS |
| ATTGCAAATGGATGGATACA+AGG | 0.498327 | 4.2:+13383436 | MS.gene08829:CDS |
| TACTTCTATGAAGCTACTGT+TGG | 0.498482 | 4.2:+13392405 | MS.gene08829:CDS |
| AGCTTTCGAAAGGAGACTCT+TGG | 0.501216 | 4.2:+13382224 | MS.gene08829:CDS |
| AATAATTCCCATTGCTATGG+TGG | 0.501521 | 4.2:-13387046 | None:intergenic |
| AGTGGAAACGGCGGGAGAGG+AGG | 0.502350 | 4.2:-13381729 | None:intergenic |
| GTTGGTGAAGCAAAGGAAGC+AGG | 0.502885 | 4.2:+13392896 | MS.gene08829:CDS |
| GATCCAAGAGATGATCTGTC+TGG | 0.502907 | 4.2:+13392932 | MS.gene08829:CDS |
| ACTCGATCAACTAAGGGATC+AGG | 0.506213 | 4.2:+13387095 | MS.gene08829:CDS |
| CCGAGCGAAGACTTGAGAAA+TGG | 0.506606 | 4.2:+13383517 | MS.gene08829:CDS |
| CTTAGCCAATGCGTTGAAAA+GGG | 0.507492 | 4.2:-13386784 | None:intergenic |
| TAATAACTTTAAAGATGGCC+GGG | 0.508147 | 4.2:+13392859 | MS.gene08829:CDS |
| GAAGACATTGTCTTATCAAG+AGG | 0.509521 | 4.2:+13383913 | MS.gene08829:CDS |
| TCCTACAACAAAATCTGTAA+AGG | 0.510625 | 4.2:-13383265 | None:intergenic |
| GTAGCAATAATTACCTTGCA+TGG | 0.510642 | 4.2:-13383552 | None:intergenic |
| CTTAGCTCTTGTAAATGTTG+AGG | 0.510677 | 4.2:+13384221 | MS.gene08829:CDS |
| AATGCTACAATTTGGGATAA+CGG | 0.512158 | 4.2:-13386712 | None:intergenic |
| AAGATAGATACCGGAGAGAG+TGG | 0.518399 | 4.2:-13381747 | None:intergenic |
| TTCATAGAAGTAGTGAGTAT+AGG | 0.519017 | 4.2:-13392394 | None:intergenic |
| AGAATGGAATTGATTGCAAA+TGG | 0.519410 | 4.2:+13383424 | MS.gene08829:intron |
| AAATTTGGTGGAACTTGTAT+GGG | 0.519729 | 4.2:+13382256 | MS.gene08829:CDS |
| TTCTGCAATGTCTAAGGTGA+CGG | 0.521017 | 4.2:+13382351 | MS.gene08829:CDS |
| TCAAGAACCACCATAGCAAT+GGG | 0.522346 | 4.2:+13387039 | MS.gene08829:CDS |
| AAAAGGGTGGCACTAACTCC+AGG | 0.522432 | 4.2:-13386768 | None:intergenic |
| AAATTTGCTCAGCACGTGCA+TGG | 0.522486 | 4.2:+13391753 | MS.gene08829:CDS |
| AAATTCGGGACCACTTGAGC+AGG | 0.528046 | 4.2:+13391893 | MS.gene08829:CDS |
| TCCAATAATTCCCATTGCTA+TGG | 0.529603 | 4.2:-13387049 | None:intergenic |
| TTGAGAGATAAAATCTGGAA+TGG | 0.530329 | 4.2:-13387020 | None:intergenic |
| AGTGAGTATAGGATTGCCTC+TGG | 0.532449 | 4.2:-13392383 | None:intergenic |
| CTTCTCCAAAACAGCATCCA+AGG | 0.533463 | 4.2:-13382431 | None:intergenic |
| GATTACTTTACCTGCTCAAG+TGG | 0.536276 | 4.2:-13391903 | None:intergenic |
| CGAATTTGCCTTCTTATTTG+GGG | 0.536880 | 4.2:-13391877 | None:intergenic |
| TCAGGCGTGTGCATCAGCTC+AGG | 0.537437 | 4.2:+13393600 | MS.gene08829:intron |
| TACTGTTGTTATTAAGCGAG+AGG | 0.538305 | 4.2:+13386972 | MS.gene08829:CDS |
| TGACGACATTGAGAATCGGC+GGG | 0.538664 | 4.2:-13381703 | None:intergenic |
| CCATTTCTCAAGTCTTCGCT+CGG | 0.540121 | 4.2:-13383517 | None:intergenic |
| ACAATTTGGACAGATGTTGA+TGG | 0.540321 | 4.2:+13383681 | MS.gene08829:CDS |
| TCTTCTTTCTGCAGTTAGTG+AGG | 0.540904 | 4.2:+13383880 | MS.gene08829:intron |
| TATAGTGCAGATCCTAGAAA+AGG | 0.542051 | 4.2:+13383708 | MS.gene08829:CDS |
| TACTATGATTGGTTGTGCAA+AGG | 0.543042 | 4.2:+13391840 | MS.gene08829:CDS |
| CTCAAGAACCACCATAGCAA+TGG | 0.545551 | 4.2:+13387038 | MS.gene08829:CDS |
| CTCAGAACAACCTTGTGCTA+TGG | 0.545614 | 4.2:-13386941 | None:intergenic |
| TCACAAAGAATAAAGAATGT+TGG | 0.549736 | 4.2:+13382283 | MS.gene08829:CDS |
| TATTCCTACTACATTGAAGC+GGG | 0.550695 | 4.2:+13383602 | MS.gene08829:CDS |
| TATGAAGCTACTGTTGGAGC+TGG | 0.552013 | 4.2:+13392411 | MS.gene08829:CDS |
| TGGAATTGATTGCAAATGGA+TGG | 0.552490 | 4.2:+13383428 | MS.gene08829:CDS |
| GCAATGGGAATTATTGGACC+AGG | 0.557880 | 4.2:+13387054 | MS.gene08829:CDS |
| GAAGTGACGACATTGAGAAT+CGG | 0.558777 | 4.2:-13381707 | None:intergenic |
| GAAAGGGAATTACGTTACCT+TGG | 0.558934 | 4.2:+13382028 | MS.gene08829:intron |
| TGTTGTTGAAGTTGTCGTGT+TGG | 0.559382 | 4.2:-13381675 | None:intergenic |
| AAATTGGTTCTTACCCGTAG+TGG | 0.559667 | 4.2:-13393501 | None:intergenic |
| GTCTAGTGCCAGAACCACTA+CGG | 0.560015 | 4.2:+13393487 | MS.gene08829:CDS |
| AATGCGAGAGAAGTGAGAGA+GGG | 0.560658 | 4.2:-13381638 | None:intergenic |
| TAGCAAGGTGGAAAGAACTT+CGG | 0.561764 | 4.2:+13391700 | MS.gene08829:CDS |
| GCAGGGGTATTGACTTAGCA+AGG | 0.562277 | 4.2:+13391685 | MS.gene08829:intron |
| ATCATCTCTTGGATCAGGCT+CGG | 0.567042 | 4.2:-13392924 | None:intergenic |
| AAACCTTGATTGCAATGCCT+CGG | 0.569657 | 4.2:-13384691 | None:intergenic |
| CCGGAGAGAGTGGAAACGGC+GGG | 0.572150 | 4.2:-13381737 | None:intergenic |
| TCTAGTGCCAGAACCACTAC+GGG | 0.573079 | 4.2:+13393488 | MS.gene08829:CDS |
| GATACCGGAGAGAGTGGAAA+CGG | 0.573419 | 4.2:-13381741 | None:intergenic |
| GAAGAGAGTCACCGAGCTCT+GGG | 0.574321 | 4.2:+13382052 | MS.gene08829:CDS |
| CCCGCCGTTTCCACTCTCTC+CGG | 0.574503 | 4.2:+13381737 | MS.gene08829:CDS |
| AAAGAACTTCGGGAGGAAAG+AGG | 0.576942 | 4.2:+13391711 | MS.gene08829:CDS |
| GTTGTCTCTAGTTATTAGGA+AGG | 0.577162 | 4.2:+13383314 | MS.gene08829:CDS |
| GGTATGCCATATCTCATCAC+AGG | 0.577975 | 4.2:-13384072 | None:intergenic |
| CCTATAGTCAGCACTTTGCG+TGG | 0.579016 | 4.2:+13392438 | MS.gene08829:CDS |
| TACAGGTGATAATTCTCGCA+AGG | 0.579848 | 4.2:+13393421 | MS.gene08829:intron |
| TGCACAACCAATCATAGTAA+TGG | 0.583719 | 4.2:-13391836 | None:intergenic |
| ATGCTTCGGGCTATATACAT+AGG | 0.590856 | 4.2:+13382544 | MS.gene08829:CDS |
| GGATGACATATCTTTGTCCC+AGG | 0.593723 | 4.2:-13384129 | None:intergenic |
| TCGCTCGGATTCCAAATAGT+CGG | 0.594375 | 4.2:-13383502 | None:intergenic |
| AACAACCTTGTGCTATGGCA+CGG | 0.594804 | 4.2:-13386936 | None:intergenic |
| TGCAGATGTTTCGGTGAATG+TGG | 0.594963 | 4.2:+13382186 | MS.gene08829:intron |
| ATGCTTTGCTGTGCCCGAGA+AGG | 0.595305 | 4.2:+13384653 | MS.gene08829:CDS |
| AACAACCACCAATTTCCTCT+CGG | 0.595610 | 4.2:-13382329 | None:intergenic |
| ATTGAGATACGTGGGAGTGG+TGG | 0.596662 | 4.2:+13394284 | MS.gene08829:CDS |
| TGAAGGAAACGACGACGCCA+TGG | 0.604386 | 4.2:-13381614 | None:intergenic |
| CTCGGTGACTCTCTTCGCCA+AGG | 0.605242 | 4.2:-13382045 | None:intergenic |
| ATCCATTTGCACAATTGTCG+GGG | 0.609087 | 4.2:+13394358 | MS.gene08829:CDS |
| GGTATTGAGATACGTGGGAG+TGG | 0.611935 | 4.2:+13394281 | MS.gene08829:intron |
| CAACTACCTCACTAAAAGCC+CGG | 0.613623 | 4.2:-13392877 | None:intergenic |
| GAAGCGCATATCCCAGAGCT+CGG | 0.618674 | 4.2:-13382063 | None:intergenic |
| CCTACTACATTGAAGCGGGA+TGG | 0.618759 | 4.2:+13383606 | MS.gene08829:CDS |
| TGTTTCGGTGAATGTGGCAG+TGG | 0.621050 | 4.2:+13382192 | MS.gene08829:CDS |
| CACTCTACTCGATCAACTAA+GGG | 0.622861 | 4.2:+13387089 | MS.gene08829:CDS |
| TCGGGAGGAAAGAGGAGAAG+CGG | 0.624010 | 4.2:+13391719 | MS.gene08829:CDS |
| TATGTATATAGCCCGAAGCA+TGG | 0.625065 | 4.2:-13382542 | None:intergenic |
| GTGACGACATTGAGAATCGG+CGG | 0.629194 | 4.2:-13381704 | None:intergenic |
| GTACTCTCTGAATCCATGCA+AGG | 0.630729 | 4.2:+13383539 | MS.gene08829:CDS |
| CAATGGGAATTATTGGACCA+GGG | 0.633816 | 4.2:+13387055 | MS.gene08829:CDS |
| ACAGGTGATAATTCTCGCAA+GGG | 0.634755 | 4.2:+13393422 | MS.gene08829:intron |
| TTCGGTGAATGTGGCAGTGG+AGG | 0.635017 | 4.2:+13382195 | MS.gene08829:CDS |
| ACCGGAGAGAGTGGAAACGG+CGG | 0.637054 | 4.2:-13381738 | None:intergenic |
| TAATTGCATTCACAACAAGA+AGG | 0.639179 | 4.2:+13394391 | MS.gene08829:CDS |
| ATATTCCTACTACATTGAAG+CGG | 0.645786 | 4.2:+13383601 | MS.gene08829:CDS |
| ATCACAGGAATTATTGTGCG+AGG | 0.646652 | 4.2:-13384057 | None:intergenic |
| TAGCTCTTGTAAATGTTGAG+GGG | 0.647826 | 4.2:+13384223 | MS.gene08829:CDS |
| AACCTACCAAACAACTCGAG+AGG | 0.648392 | 4.2:-13394568 | None:intergenic |
| TTGCAAATGGATGGATACAA+GGG | 0.649756 | 4.2:+13383437 | MS.gene08829:CDS |
| CACGGCATTAGTAGACTGCA+CGG | 0.650478 | 4.2:+13391794 | MS.gene08829:CDS |
| AATGTCCGTGCCATAGCACA+AGG | 0.651217 | 4.2:+13386931 | MS.gene08829:CDS |
| AGCCAATGCGTTGAAAAGGG+TGG | 0.651417 | 4.2:-13386781 | None:intergenic |
| GGAAGTAAAAGCTGTTGCCG+AGG | 0.655331 | 4.2:+13384674 | MS.gene08829:CDS |
| AGAGCTGCGAAGATACAAGA+AGG | 0.656222 | 4.2:+13394332 | MS.gene08829:CDS |
| TCTTTGTCCCAGGAGCAGAA+AGG | 0.657330 | 4.2:-13384119 | None:intergenic |
| TGAGGTTAAGAGCTCTCCAG+AGG | 0.660123 | 4.2:+13392367 | MS.gene08829:CDS |
| TCAACTTGATTAGAACCAGT+AGG | 0.662926 | 4.2:-13383477 | None:intergenic |
| TGATTCCGAGAGGAAATTGG+TGG | 0.664157 | 4.2:+13382324 | MS.gene08829:CDS |
| AATAACTAGAGACAACATCT+GGG | 0.664378 | 4.2:-13383307 | None:intergenic |
| TTGTAGGTATTGAGATACGT+GGG | 0.666439 | 4.2:+13394276 | MS.gene08829:intron |
| TTTGTAGGTATTGAGATACG+TGG | 0.669349 | 4.2:+13394275 | MS.gene08829:intron |
| AGAGTGCTCCCAATTAACCC+TGG | 0.678345 | 4.2:-13387072 | None:intergenic |
| ACAGCTACCAAAATTTGACC+CGG | 0.679982 | 4.2:+13393633 | MS.gene08829:CDS |
| AGAAGATGCTGACAATGCTG+GGG | 0.682028 | 4.2:+13393672 | MS.gene08829:CDS |
| GACGACATTGAGAATCGGCG+GGG | 0.702844 | 4.2:-13381702 | None:intergenic |
| AAGGTGGAAAGAACTTCGGG+AGG | 0.708619 | 4.2:+13391704 | MS.gene08829:CDS |
| TTCTCAATGATGATTCCGAG+AGG | 0.724505 | 4.2:+13382314 | MS.gene08829:CDS |
| GGGGTATTGACTTAGCAAGG+TGG | 0.731508 | 4.2:+13391688 | MS.gene08829:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 13381616 | 13395504 | 13381616 | ID=MS.gene08829 |
| chr4.2 | mRNA | 13381616 | 13395504 | 13381616 | ID=MS.gene08829.t1;Parent=MS.gene08829 |
| chr4.2 | exon | 13381616 | 13381758 | 13381616 | ID=MS.gene08829.t1.exon1;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13381616 | 13381758 | 13381616 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13382029 | 13382093 | 13382029 | ID=MS.gene08829.t1.exon2;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13382029 | 13382093 | 13382029 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13382191 | 13382565 | 13382191 | ID=MS.gene08829.t1.exon3;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13382191 | 13382565 | 13382191 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13383247 | 13383335 | 13383247 | ID=MS.gene08829.t1.exon4;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13383247 | 13383335 | 13383247 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13383426 | 13383729 | 13383426 | ID=MS.gene08829.t1.exon5;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13383426 | 13383729 | 13383426 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13383894 | 13383946 | 13383894 | ID=MS.gene08829.t1.exon6;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13383894 | 13383946 | 13383894 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13384030 | 13384244 | 13384030 | ID=MS.gene08829.t1.exon7;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13384030 | 13384244 | 13384030 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13384404 | 13384491 | 13384404 | ID=MS.gene08829.t1.exon8;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13384404 | 13384491 | 13384404 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13384633 | 13384746 | 13384633 | ID=MS.gene08829.t1.exon9;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13384633 | 13384746 | 13384633 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13386708 | 13386806 | 13386708 | ID=MS.gene08829.t1.exon10;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13386708 | 13386806 | 13386708 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13386922 | 13387116 | 13386922 | ID=MS.gene08829.t1.exon11;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13386922 | 13387116 | 13386922 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13390784 | 13390866 | 13390784 | ID=MS.gene08829.t1.exon12;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13390784 | 13390866 | 13390784 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13391689 | 13391914 | 13391689 | ID=MS.gene08829.t1.exon13;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13391689 | 13391914 | 13391689 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13392363 | 13392508 | 13392363 | ID=MS.gene08829.t1.exon14;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13392363 | 13392508 | 13392363 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13392838 | 13392973 | 13392838 | ID=MS.gene08829.t1.exon15;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13392838 | 13392973 | 13392838 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13393426 | 13393509 | 13393426 | ID=MS.gene08829.t1.exon16;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13393426 | 13393509 | 13393426 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13393604 | 13393696 | 13393604 | ID=MS.gene08829.t1.exon17;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13393604 | 13393696 | 13393604 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13394282 | 13394412 | 13394282 | ID=MS.gene08829.t1.exon18;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13394282 | 13394412 | 13394282 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13394528 | 13394583 | 13394528 | ID=MS.gene08829.t1.exon19;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13394528 | 13394583 | 13394528 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| chr4.2 | exon | 13395494 | 13395504 | 13395494 | ID=MS.gene08829.t1.exon20;Parent=MS.gene08829.t1 |
| chr4.2 | CDS | 13395494 | 13395504 | 13395494 | ID=cds.MS.gene08829.t1;Parent=MS.gene08829.t1 |
| Gene Sequence |
| Protein sequence |
