AlfalfaGEDB | MS.gene08896

M. sativa cultivar XinJiangDaYe / MS.gene08896

KEGG: K01166
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0004521 GO:0004540 GO:0005488 GO:0016787 GO:0016788 GO:0016892 GO:0016894 GO:0033897 GO:0097159 GO:0140098 GO:1901363
PFAM: PF00445.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08896.t1	PNY16784.1	53.3	184	77	8	1	177	1	182	2.70E-45	191.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08896.t1	P80196	33.3	192	110	9	4	177	35	226	5.3e-21	102.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08896.t1	A0A2K3PNE6	53.3	184	77	8	1	177	1	182	2.0e-45	191.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050092	MS.gene08896	0.80936	1.97E-50	-1.69E-46
MS.gene050101	MS.gene08896	0.804431	2.17E-49	-1.69E-46
MS.gene050102	MS.gene08896	0.820261	7.53E-53	-1.69E-46
MS.gene052187	MS.gene08896	0.804576	2.02E-49	-1.69E-46
MS.gene052456	MS.gene08896	0.850406	1.73E-60	-1.69E-46
MS.gene053383	MS.gene08896	0.803251	3.81E-49	-1.69E-46
MS.gene055359	MS.gene08896	0.859493	3.98E-63	-1.69E-46
MS.gene05578	MS.gene08896	0.809432	1.90E-50	-1.69E-46
MS.gene059632	MS.gene08896	0.820758	5.79E-53	-1.69E-46
MS.gene061081	MS.gene08896	0.80066	1.30E-48	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08896.t1	MTR_4g057825	38.710	186	106	5	1	181	25	207	3.59e-40	135
MS.gene08896.t1	MTR_3g030660	34.211	190	111	9	2	177	84	273	2.32e-28	107
MS.gene08896.t1	MTR_3g030640	34.737	190	110	9	2	177	79	268	2.79e-28	106
MS.gene08896.t1	MTR_2g021750	36.000	175	98	7	3	166	163	334	5.02e-28	107
MS.gene08896.t1	MTR_6g071425	35.859	198	104	9	2	176	83	280	1.29e-26	102
MS.gene08896.t1	MTR_5g040960	33.838	198	111	10	2	179	33	230	7.35e-26	99.4
MS.gene08896.t1	MTR_2g021830	31.667	180	113	7	4	176	13	189	9.83e-26	97.8
MS.gene08896.t1	MTR_2g021910	34.161	161	100	5	2	158	250	408	1.34e-25	102
MS.gene08896.t1	MTR_2g021910	30.814	172	111	5	2	167	547	716	6.54e-21	89.4
MS.gene08896.t1	MTR_6g071505	31.959	194	113	8	2	176	76	269	3.44e-24	95.9
MS.gene08896.t1	MTR_5g022810	30.964	197	111	10	6	177	33	229	3.86e-23	92.0
MS.gene08896.t1	MTR_5g041010	31.633	196	116	10	2	179	32	227	1.63e-22	90.5
MS.gene08896.t1	MTR_5g040940	32.474	194	113	11	2	177	33	226	2.16e-22	90.1
MS.gene08896.t1	MTR_5g086410	33.679	193	110	10	1	177	24	214	1.29e-21	87.8
MS.gene08896.t1	MTR_6g090200	30.601	183	116	5	1	175	26	205	9.74e-21	85.5
MS.gene08896.t1	MTR_1g048200	33.854	192	100	11	2	170	109	296	4.12e-20	85.5
MS.gene08896.t1	MTR_0002s1200	33.333	198	105	9	1	177	108	299	4.29e-20	85.5
MS.gene08896.t1	MTR_0129s0080	33.514	185	102	11	2	166	30	213	3.09e-17	76.6
MS.gene08896.t1	MTR_5g041080	29.744	195	119	10	2	178	34	228	7.21e-17	75.9
MS.gene08896.t1	MTR_5g041040	28.061	196	123	10	2	179	33	228	6.63e-16	73.2
MS.gene08896.t1	MTR_2g104370	29.101	189	107	10	10	179	19	199	5.89e-15	70.1
MS.gene08896.t1	MTR_8g013025	33.077	130	69	6	2	117	303	428	7.48e-13	66.2
MS.gene08896.t1	MTR_2g104300	32.727	110	57	5	1	99	31	134	2.63e-12	61.6
MS.gene08896.t1	MTR_5g086770	30.070	143	88	5	19	153	58	196	1.78e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08896.t1	AT2G02990	32.474	194	113	10	2	177	35	228	8.54e-21	86.3
MS.gene08896.t1	AT1G26820	30.208	192	116	10	6	179	31	222	3.61e-19	81.6
MS.gene08896.t1	AT2G39780	27.184	206	118	8	2	178	37	239	4.12e-16	73.9
MS.gene08896.t1	AT1G14220	31.250	176	104	9	6	164	37	212	5.31e-16	73.2
MS.gene08896.t1	AT1G14210	27.835	194	120	10	6	179	35	228	3.53e-14	68.6

Find 46 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGATCTTTGATGTCATTT+AGG	0.156708	4.4:+45626279	None:intergenic
GTCTCATTTGTGCAATGATA+TGG	0.225299	4.4:+45626559	None:intergenic
AAGATCTTTGATGTCATTTA+GGG	0.233880	4.4:+45626280	None:intergenic
CCACAATTTGTACCAGTATT+AGG	0.259803	4.4:+45626089	None:intergenic
CATTTAGGGCATACAAATCT+AGG	0.293125	4.4:+45626294	None:intergenic
CGGGAAACAACTCTGACTTT+TGG	0.314940	4.4:-45626382	MS.gene08896:CDS
TGCCCGGGATTGATAAGATT+TGG	0.330378	4.4:-45626415	MS.gene08896:CDS
TAACTTTATTTAATAACTTC+TGG	0.331114	4.4:+45626050	None:intergenic
TTATTATCACAATGAAGCTT+AGG	0.393545	4.4:+45626176	None:intergenic
TACCAGCTTGATCCATTGCC+CGG	0.424603	4.4:-45626431	MS.gene08896:intron
TATATAAGTTGTCCTAATAC+TGG	0.424971	4.4:-45626101	MS.gene08896:CDS
TTTGTGCAATGATATGGGTT+AGG	0.440239	4.4:+45626565	None:intergenic
GAGTTGTTTCCCGTTAGATT+CGG	0.449794	4.4:+45626392	None:intergenic
GTAATCGTATGGTGACATAT+TGG	0.450909	4.4:+45626328	None:intergenic
TATTATCACAATGAAGCTTA+GGG	0.458506	4.4:+45626177	None:intergenic
AACTTTAACACCTGGTGGAT+CGG	0.470033	4.4:-45626249	MS.gene08896:CDS
AAGATTTGGCCGAATCTAAC+GGG	0.475239	4.4:-45626401	MS.gene08896:CDS
CTTAATAAAACTTTAACACC+TGG	0.479447	4.4:-45626257	MS.gene08896:CDS
CGGCCAAATCTTATCAATCC+CGG	0.483167	4.4:+45626412	None:intergenic
ATCGAGAGTGCTATAAAGTT+AGG	0.484561	4.4:-45626212	MS.gene08896:CDS
CCTAATACTGGTACAAATTG+TGG	0.488261	4.4:-45626089	MS.gene08896:CDS
TCTCATTTGTGCAATGATAT+GGG	0.503124	4.4:+45626560	None:intergenic
ATCTTATCAATCCCGGGCAA+TGG	0.515207	4.4:+45626419	None:intergenic
ATTTCAGCATCCACGGATTG+TGG	0.518420	4.4:-45626606	MS.gene08896:CDS
TCTGCTTCAATACCACCAAC+AGG	0.519484	4.4:-45626124	MS.gene08896:CDS
TGTTCTGCGGCCACAATCCG+TGG	0.527412	4.4:+45626596	None:intergenic
TAAGATTTGGCCGAATCTAA+CGG	0.533520	4.4:-45626402	MS.gene08896:CDS
CCTCAAAATTTCAGCATCCA+CGG	0.533572	4.4:-45626613	MS.gene08896:CDS
GGCCAAATCTTATCAATCCC+GGG	0.549058	4.4:+45626413	None:intergenic
AATAAAACTTTAACACCTGG+TGG	0.549310	4.4:-45626254	MS.gene08896:CDS
ATGAAGCTTAGGGTTAGATC+CGG	0.566541	4.4:+45626187	None:intergenic
ACCAGCTTGATCCATTGCCC+GGG	0.575199	4.4:-45626430	MS.gene08896:intron
ATTTAGGGCATACAAATCTA+GGG	0.575925	4.4:+45626295	None:intergenic
GCTTTCGTCGCCGATCCACC+AGG	0.584621	4.4:+45626239	None:intergenic
GATTGTGGCCGCAGAACAAG+AGG	0.588738	4.4:-45626591	MS.gene08896:CDS
AGGACAACTTATATACCTGT+TGG	0.591332	4.4:+45626109	None:intergenic
ACAACTTATATACCTGTTGG+TGG	0.612648	4.4:+45626112	None:intergenic
GCAAGCTCGAAGTAATCGTA+TGG	0.614270	4.4:+45626317	None:intergenic
TTAGGCTTCCTCTTGTTCTG+CGG	0.625435	4.4:+45626583	None:intergenic
AGTGCTATAAAGTTAGGCAC+CGG	0.626348	4.4:-45626206	MS.gene08896:CDS
TCATTGCACAAATGAGACAC+TGG	0.631016	4.4:-45626554	MS.gene08896:CDS
ACTAGATGCTAGCAGAAACA+TGG	0.637267	4.4:-45626678	None:intergenic
TGAGACACTGGAGCCGCATG+TGG	0.638823	4.4:-45626542	MS.gene08896:intron
TCGAGATAGCATACCACATG+CGG	0.641657	4.4:+45626529	None:intergenic
GCTTACGAGTGGAAGATACA+TGG	0.644977	4.4:-45626359	MS.gene08896:CDS
TAACTTTATAGCACTCTCGA+TGG	0.706282	4.4:+45626214	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTACTATTTTTATCTTA+TGG	-	chr4.4:45626235-45626254	MS.gene08896:CDS	10.0%
!!	ATAGTAAAATCAAAGATAAT+TGG	+	chr4.4:45626225-45626244	None:intergenic	15.0%
!!	TAAAAACTCTAGTAATTTGA+TGG	-	chr4.4:45626572-45626591	MS.gene08896:CDS	20.0%
!!!	TGAAATTTTGAGGTATATTA+GGG	+	chr4.4:45626108-45626127	None:intergenic	20.0%
!	CTTAATAAAACTTTAACACC+TGG	-	chr4.4:45626471-45626490	MS.gene08896:intron	25.0%
!	TATATAAGTTGTCCTAATAC+TGG	-	chr4.4:45626627-45626646	MS.gene08896:CDS	25.0%
!	TATTATCACAATGAAGCTTA+GGG	+	chr4.4:45626554-45626573	None:intergenic	25.0%
!	TTATTATCACAATGAAGCTT+AGG	+	chr4.4:45626555-45626574	None:intergenic	25.0%
!!	AAGATCTTTGATGTCATTTA+GGG	+	chr4.4:45626451-45626470	None:intergenic	25.0%
!!	GATTTGTTTAGACAAAAAGT+TGG	+	chr4.4:45626079-45626098	None:intergenic	25.0%
!!!	CTATTTTTATCTTATGGTGT+TGG	-	chr4.4:45626241-45626260	MS.gene08896:CDS	25.0%
!!!	CTGAAATTTTGAGGTATATT+AGG	+	chr4.4:45626109-45626128	None:intergenic	25.0%
	AATAAAACTTTAACACCTGG+TGG	-	chr4.4:45626474-45626493	MS.gene08896:intron	30.0%
	TCTCATTTGTGCAATGATAT+GGG	+	chr4.4:45626171-45626190	None:intergenic	30.0%
	TTGTTTAGACAAAAAGTTGG+TGG	+	chr4.4:45626076-45626095	None:intergenic	30.0%
!	ATTTAGGGCATACAAATCTA+GGG	+	chr4.4:45626436-45626455	None:intergenic	30.0%
!	GAAGATCTTTGATGTCATTT+AGG	+	chr4.4:45626452-45626471	None:intergenic	30.0%
	ACAACTTATATACCTGTTGG+TGG	+	chr4.4:45626619-45626638	None:intergenic	35.0%
	AGGACAACTTATATACCTGT+TGG	+	chr4.4:45626622-45626641	None:intergenic	35.0%
	CCACAATTTGTACCAGTATT+AGG	+	chr4.4:45626642-45626661	None:intergenic	35.0%
	CCTAATACTGGTACAAATTG+TGG	-	chr4.4:45626639-45626658	MS.gene08896:CDS	35.0%
	GTAATCGTATGGTGACATAT+TGG	+	chr4.4:45626403-45626422	None:intergenic	35.0%
	GTCTCATTTGTGCAATGATA+TGG	+	chr4.4:45626172-45626191	None:intergenic	35.0%
	TAACTTTATAGCACTCTCGA+TGG	+	chr4.4:45626517-45626536	None:intergenic	35.0%
	TAAGATTTGGCCGAATCTAA+CGG	-	chr4.4:45626326-45626345	MS.gene08896:CDS	35.0%
	TTTGTGCAATGATATGGGTT+AGG	+	chr4.4:45626166-45626185	None:intergenic	35.0%
!	CATTTAGGGCATACAAATCT+AGG	+	chr4.4:45626437-45626456	None:intergenic	35.0%
!!	ATCGAGAGTGCTATAAAGTT+AGG	-	chr4.4:45626516-45626535	MS.gene08896:intron	35.0%
	AAGATTTGGCCGAATCTAAC+GGG	-	chr4.4:45626327-45626346	MS.gene08896:CDS	40.0%
	AGTGCTATAAAGTTAGGCAC+CGG	-	chr4.4:45626522-45626541	MS.gene08896:intron	40.0%
	ATGAAGCTTAGGGTTAGATC+CGG	+	chr4.4:45626544-45626563	None:intergenic	40.0%
	CCTCAAAATTTCAGCATCCA+CGG	-	chr4.4:45626115-45626134	MS.gene08896:CDS	40.0%
	TCATTGCACAAATGAGACAC+TGG	-	chr4.4:45626174-45626193	MS.gene08896:CDS	40.0%
!	AACTTTAACACCTGGTGGAT+CGG	-	chr4.4:45626479-45626498	MS.gene08896:intron	40.0%
!	GAGTTGTTTCCCGTTAGATT+CGG	+	chr4.4:45626339-45626358	None:intergenic	40.0%
!	GGGAAACAACTCTGACTTTT+GGG	-	chr4.4:45626347-45626366	MS.gene08896:CDS	40.0%
	ATCTTATCAATCCCGGGCAA+TGG	+	chr4.4:45626312-45626331	None:intergenic	45.0%
	CGGCCAAATCTTATCAATCC+CGG	+	chr4.4:45626319-45626338	None:intergenic	45.0%
	GCAAGCTCGAAGTAATCGTA+TGG	+	chr4.4:45626414-45626433	None:intergenic	45.0%
	GCTTACGAGTGGAAGATACA+TGG	-	chr4.4:45626369-45626388	MS.gene08896:CDS	45.0%
	GGCCAAATCTTATCAATCCC+GGG	+	chr4.4:45626318-45626337	None:intergenic	45.0%
	TCGAGATAGCATACCACATG+CGG	+	chr4.4:45626202-45626221	None:intergenic	45.0%
	TCTGCTTCAATACCACCAAC+AGG	-	chr4.4:45626604-45626623	MS.gene08896:CDS	45.0%
	TGCCCGGGATTGATAAGATT+TGG	-	chr4.4:45626313-45626332	MS.gene08896:CDS	45.0%
	TTAGGCTTCCTCTTGTTCTG+CGG	+	chr4.4:45626148-45626167	None:intergenic	45.0%
!	ATTTCAGCATCCACGGATTG+TGG	-	chr4.4:45626122-45626141	MS.gene08896:CDS	45.0%
!	CCGTGGATGCTGAAATTTTG+AGG	+	chr4.4:45626118-45626137	None:intergenic	45.0%
!	CGGGAAACAACTCTGACTTT+TGG	-	chr4.4:45626346-45626365	MS.gene08896:CDS	45.0%
	TACCAGCTTGATCCATTGCC+CGG	-	chr4.4:45626297-45626316	MS.gene08896:CDS	50.0%
!	CTGACTTTTGGGCTTACGAG+TGG	-	chr4.4:45626358-45626377	MS.gene08896:CDS	50.0%
	ACCAGCTTGATCCATTGCCC+GGG	-	chr4.4:45626298-45626317	MS.gene08896:CDS	55.0%
	GATTGTGGCCGCAGAACAAG+AGG	-	chr4.4:45626137-45626156	MS.gene08896:CDS	55.0%
	TCCCGGGCAATGGATCAAGC+TGG	+	chr4.4:45626302-45626321	None:intergenic	60.0%
	TGAGACACTGGAGCCGCATG+TGG	-	chr4.4:45626186-45626205	MS.gene08896:CDS	60.0%
	TGTTCTGCGGCCACAATCCG+TGG	+	chr4.4:45626135-45626154	None:intergenic	60.0%
	GCTTTCGTCGCCGATCCACC+AGG	+	chr4.4:45626492-45626511	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	45626055	45626695	45626055	ID=MS.gene08896
chr4.4	mRNA	45626055	45626695	45626055	ID=MS.gene08896.t1;Parent=MS.gene08896
chr4.4	exon	45626543	45626695	45626543	ID=MS.gene08896.t1.exon1;Parent=MS.gene08896.t1
chr4.4	CDS	45626543	45626695	45626543	ID=cds.MS.gene08896.t1;Parent=MS.gene08896.t1
chr4.4	exon	45626055	45626447	45626055	ID=MS.gene08896.t1.exon2;Parent=MS.gene08896.t1
chr4.4	CDS	45626055	45626447	45626055	ID=cds.MS.gene08896.t1;Parent=MS.gene08896.t1

Gene Sequence

>MS.gene08896

ATGCTAGCAGAAACATGGCCACCAACTTTTTGTCTAAACAAATCATGTGTCCCTAATATACCTCAAAATTTCAGCATCCACGGATTGTGGCCGCAGAACAAGAGGAAGCCTAACCCATATCATTGCACAAATGAGACACTGGAGCCGCATGTGGTATGCTATCTCGACCAATTATCTTTGATTTTACTATTTTTATCTTATGGTGTTGGAGATATAATCATTTACTTTATTTTATTTTATTTTACCAGCTTGATCCATTGCCCGGGATTGATAAGATTTGGCCGAATCTAACGGGAAACAACTCTGACTTTTGGGCTTACGAGTGGAAGATACATGGTACTTGCTCCAATATGTCACCATACGATTACTTCGAGCTTGCCCTAGATTTGTATGCCCTAAATGACATCAAAGATCTTCTTAATAAAACTTTAACACCTGGTGGATCGGCGACGAAAGCTGCCATCGAGAGTGCTATAAAGTTAGGCACCGGATCTAACCCTAAGCTTCATTGTGATAATAAAAACTCTAGTAATTTGATGGAAGTTAGACTCTGCTTCAATACCACCAACAGGTATATAAGTTGTCCTAATACTGGTACAAATTGTGGAGCTACAATACATTTACCAGAAGTTATTAAATAA

Protein sequence

>MS.gene08896.t1

MLAETWPPTFCLNKSCVPNIPQNFSIHGLWPQNKRKPNPYHCTNETLEPHVLDPLPGIDKIWPNLTGNNSDFWAYEWKIHGTCSNMSPYDYFELALDLYALNDIKDLLNKTLTPGGSATKAAIESAIKLGTGSNPKLHCDNKNSSNLMEVRLCFNTTNRYISCPNTGTNCGATIHLPEVIK