AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09172

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09172.t1 MTR_4g057500 98.020 303 6 0 8 310 163 465 0.0 619
MS.gene09172.t1 MTR_2g084245 81.940 299 54 0 8 306 145 443 0.0 521
MS.gene09172.t1 MTR_8g096140 40.893 291 166 4 8 296 107 393 3.73e-76 239
MS.gene09172.t1 MTR_8g096160 40.206 291 168 4 8 296 107 393 2.18e-75 237
MS.gene09172.t1 MTR_7g103270 29.924 264 163 5 27 289 147 389 6.05e-31 120
MS.gene09172.t1 MTR_1g061760 28.470 281 152 9 24 289 229 475 7.95e-28 112
MS.gene09172.t1 MTR_4g100730 29.921 254 155 5 34 277 285 525 2.19e-27 112
MS.gene09172.t1 MTR_7g103260 29.323 266 162 6 27 289 146 388 4.84e-27 109
MS.gene09172.t1 MTR_3g097310 28.261 230 147 4 30 259 243 454 9.19e-25 104
MS.gene09172.t1 MTR_4g115530 27.203 261 170 5 29 289 87 327 9.40e-25 102
MS.gene09172.t1 MTR_3g106690 29.740 269 155 7 29 289 140 382 1.71e-24 102
MS.gene09172.t1 MTR_4g115530 27.203 261 170 5 29 289 141 381 1.86e-24 102
MS.gene09172.t1 MTR_5g031480 26.667 270 160 7 29 289 166 406 3.49e-24 102
MS.gene09172.t1 MTR_4g115540 27.376 263 167 7 29 289 375 615 1.88e-23 100
MS.gene09172.t1 MTR_3g080690 26.966 267 161 9 30 289 196 435 2.64e-23 99.8
MS.gene09172.t1 MTR_3g081240 26.217 267 163 8 30 289 198 437 1.12e-22 97.8
MS.gene09172.t1 MTR_5g049790 26.515 264 152 7 29 289 202 426 1.16e-21 94.7
MS.gene09172.t1 MTR_4g115460 26.357 258 164 6 36 289 218 453 1.01e-20 92.4
MS.gene09172.t1 MTR_7g103150 26.224 286 166 8 17 289 223 476 1.67e-20 92.0
MS.gene09172.t1 MTR_8g102840 29.588 267 148 10 27 277 357 599 2.38e-20 91.7
MS.gene09172.t1 MTR_1g090733 29.771 262 148 9 30 277 298 537 5.63e-20 90.5
MS.gene09172.t1 MTR_3g110198 25.735 272 159 7 30 289 345 585 6.72e-20 90.5
MS.gene09172.t1 MTR_7g085840 26.374 273 167 10 22 289 75 318 2.35e-19 87.4
MS.gene09172.t1 MTR_1g006600 26.766 269 152 8 34 289 342 578 6.16e-19 87.4
MS.gene09172.t1 MTR_1g052730 26.415 265 142 10 37 261 318 569 7.83e-18 84.3
MS.gene09172.t1 MTR_1g052730 26.415 265 142 10 37 261 466 717 1.11e-17 84.0
MS.gene09172.t1 MTR_4g077920 25.758 264 175 7 3 261 129 376 1.61e-17 82.8
MS.gene09172.t1 MTR_4g077920 25.620 242 162 6 25 261 206 434 1.64e-16 80.1
MS.gene09172.t1 MTR_4g077920 25.620 242 162 6 25 261 206 434 2.10e-16 79.7
MS.gene09172.t1 MTR_3g110200 24.912 285 159 8 25 289 333 582 2.41e-16 79.7
MS.gene09172.t1 MTR_5g031480 26.562 192 105 6 29 211 166 330 3.38e-13 69.3
MS.gene09172.t1 MTR_5g031480 27.083 192 102 7 29 210 166 329 1.14e-12 68.2
MS.gene09172.t1 MTR_6g027310 25.641 273 171 10 29 293 221 469 4.05e-12 67.0
MS.gene09172.t1 MTR_1g097090 25.869 259 144 8 44 279 195 428 4.57e-12 66.6
MS.gene09172.t1 MTR_1g097090 25.869 259 144 8 44 279 195 428 5.71e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09172.t1 AT2G28110 75.427 293 72 0 8 300 152 444 2.31e-172 485
MS.gene09172.t1 AT5G22940 71.287 303 86 1 8 309 166 468 2.49e-162 461
MS.gene09172.t1 AT5G22940 71.287 303 86 1 8 309 195 497 2.88e-162 461
MS.gene09172.t1 AT1G27440 39.860 286 168 3 8 293 103 384 5.34e-75 236
MS.gene09172.t1 AT5G61840 39.510 286 169 3 8 293 106 387 1.68e-74 234
MS.gene09172.t1 AT4G16745 33.588 262 135 10 34 277 281 521 1.18e-31 124
MS.gene09172.t1 AT4G16745 33.992 253 128 10 34 268 281 512 1.14e-30 121
MS.gene09172.t1 AT5G11130 29.670 273 168 6 21 289 71 323 5.01e-28 111
MS.gene09172.t1 AT5G11130 29.710 276 164 7 21 289 215 467 2.11e-27 111
MS.gene09172.t1 AT5G33290 28.767 292 176 9 2 289 226 489 3.34e-25 105
MS.gene09172.t1 AT3G42180 26.937 271 158 7 29 289 90 330 1.08e-24 102
MS.gene09172.t1 AT5G03795 28.571 266 164 5 27 289 264 506 2.22e-24 103
MS.gene09172.t1 AT3G42180 27.675 271 156 8 29 289 219 459 2.26e-24 102
MS.gene09172.t1 AT4G38040 27.778 270 157 8 29 289 176 416 2.42e-24 102
MS.gene09172.t1 AT5G20260 28.682 258 161 7 34 289 88 324 2.83e-24 100
MS.gene09172.t1 AT5G20260 28.682 258 161 7 34 289 115 351 3.96e-24 100
MS.gene09172.t1 AT5G20260 27.863 262 158 8 34 289 220 456 6.66e-24 101
MS.gene09172.t1 AT4G38040 28.512 242 137 7 29 261 176 390 1.94e-23 99.8
MS.gene09172.t1 AT5G19670 28.358 268 150 10 27 277 355 597 4.69e-22 96.7
MS.gene09172.t1 AT5G19670 28.358 268 150 10 27 277 355 597 4.69e-22 96.7
MS.gene09172.t1 AT5G37000 31.963 219 121 6 34 246 306 502 9.09e-22 95.9
MS.gene09172.t1 AT5G25310 26.022 269 170 6 27 289 225 470 3.26e-20 90.9
MS.gene09172.t1 AT3G07620 25.660 265 169 7 29 289 209 449 7.83e-20 89.7
MS.gene09172.t1 AT5G25820 27.586 261 162 9 34 289 406 644 2.80e-19 88.6
MS.gene09172.t1 AT5G11610 27.891 294 172 10 2 289 278 537 8.30e-19 87.0
MS.gene09172.t1 AT3G57630 28.175 252 152 7 33 256 456 706 1.60e-18 86.7
MS.gene09172.t1 AT3G57630 28.175 252 152 7 33 256 456 706 1.60e-18 86.7
MS.gene09172.t1 AT3G57630 28.175 252 152 7 33 256 454 704 1.74e-18 86.3
MS.gene09172.t1 AT4G32790 26.263 297 176 9 2 289 323 585 1.74e-18 86.3
MS.gene09172.t1 AT4G32790 26.263 297 176 9 2 289 323 585 1.74e-18 86.3
MS.gene09172.t1 AT4G32790 26.263 297 176 9 2 289 323 585 1.74e-18 86.3
MS.gene09172.t1 AT5G16890 25.623 281 164 12 25 286 212 466 3.23e-16 79.3
MS.gene09172.t1 AT3G45400 28.017 232 142 9 14 241 177 387 4.48e-12 67.0
MS.gene09172.t1 AT3G03650 25.000 236 154 8 14 246 216 431 2.93e-11 64.3

Find 93 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAACTTCAGCACCGTTAA+TGG 0.202940 4.3:-43636620 MS.gene09172:CDS
TGAACGGACGGCGAGATATT+TGG 0.204415 4.3:-43636179 MS.gene09172:CDS
ATTGAATTCTTCATAATTTC+AGG 0.233449 4.3:+43636318 None:intergenic
TCTCCACCGAGTACCCTTTC+TGG 0.234469 4.3:-43636544 MS.gene09172:CDS
ATCGTATTCCGTACACTTTC+CGG 0.254767 4.3:+43636216 None:intergenic
ATTACGTGTCAAATTGTAAT+TGG 0.266113 4.3:+43635221 None:intergenic
AACCTTCCACTAACGTTCTT+AGG 0.284742 4.3:+43636126 None:intergenic
AAAAGGTGGATAGGTCTTAT+AGG 0.332673 4.3:-43635261 MS.gene09172:CDS
CTAGTTGAATCCGTTGCATT+AGG 0.341394 4.3:-43635535 MS.gene09172:CDS
GAACGGACGGCGAGATATTT+GGG 0.347966 4.3:-43636178 MS.gene09172:CDS
CTACGGAGACATAGGTTTGC+CGG 0.360281 4.3:-43635628 MS.gene09172:CDS
GGAAAGTGAGAACAGTGATA+TGG 0.375416 4.3:-43635681 MS.gene09172:CDS
AATTACAATTTGACACGTAA+TGG 0.378306 4.3:-43635219 None:intergenic
TGCAATTTCTGACTGGTAAC+CGG 0.384819 4.3:+43635609 None:intergenic
GTCCTTTGGGGTGGGCCCCA+TGG 0.390095 4.3:-43635567 MS.gene09172:CDS
TCAATTGTGTTACAAACGTT+TGG 0.390232 4.3:-43636300 MS.gene09172:CDS
TTCGTAGCTTCCCATGATTT+CGG 0.390850 4.3:-43636494 MS.gene09172:CDS
TAATCATAGCGGACGGTATC+CGG 0.403149 4.3:-43635501 MS.gene09172:CDS
CGGCGGAGGAAAACGGTAAC+CGG 0.425078 4.3:+43635482 None:intergenic
GAAAACAAGAACCGAAATCA+TGG 0.441468 4.3:+43636483 None:intergenic
CCAGTCAGAAATTGCACGTT+CGG 0.444334 4.3:-43635602 MS.gene09172:CDS
CCTACATTCTTTGAGTGAAA+AGG 0.453638 4.3:-43635278 MS.gene09172:CDS
GCCACGTGGCATCTCCTTCG+TGG 0.462330 4.3:+43635305 None:intergenic
GAACGTGCATGACCTATTGC+TGG 0.469918 4.3:+43636593 None:intergenic
CATTATTCAAAGAAACTTGT+GGG 0.471177 4.3:-43635368 MS.gene09172:CDS
TTGTTTCGCCGGAAAGTGTA+CGG 0.472394 4.3:-43636224 MS.gene09172:CDS
ATACCGTCCGCTATGATTAC+CGG 0.473599 4.3:+43635505 None:intergenic
GTTAATGGCTTCCCAGCAAT+AGG 0.474523 4.3:-43636605 MS.gene09172:CDS
GGAGAAAGATGTTTGGAGGC+TGG 0.479889 4.3:-43635428 MS.gene09172:CDS
CGGTGGCGGAGAAAGATGTT+TGG 0.483620 4.3:-43635435 MS.gene09172:CDS
TTGTTTATGTCCTTTGGGGT+GGG 0.483838 4.3:-43635575 MS.gene09172:CDS
ATATCTCGCCGTCCGTTCAC+CGG 0.484215 4.3:+43636183 None:intergenic
CGAAGGAGATGCCACGTGGC+AGG 0.493641 4.3:-43635302 MS.gene09172:CDS
TGAAATCTCCGGCCAGTTCA+CGG 0.495124 4.3:+43635462 None:intergenic
GAGGACGTGGCAATGAAAGA+TGG 0.495923 4.3:-43636345 MS.gene09172:CDS
ATAGGTCTTATAGGAGTTCA+AGG 0.496139 4.3:-43635252 MS.gene09172:CDS
TATTGCTGGGAAGCCATTAA+CGG 0.499750 4.3:+43636607 None:intergenic
CAGTTCACGGCGGAGGAAAA+CGG 0.502991 4.3:+43635475 None:intergenic
GAGAAAGATGTTTGGAGGCT+GGG 0.503317 4.3:-43635427 MS.gene09172:CDS
GATACCACCGTTTGTTTCGC+CGG 0.503663 4.3:-43636235 MS.gene09172:CDS
CTGTGCTACGGTTCCAGAAA+GGG 0.508197 4.3:+43636531 None:intergenic
TTCCTCCGCCGTGAACTGGC+CGG 0.509651 4.3:-43635470 MS.gene09172:CDS
CTCCGGCCAGTTCACGGCGG+AGG 0.510218 4.3:+43635468 None:intergenic
GTTCATCCTAAGAACGTTAG+TGG 0.510459 4.3:-43636132 MS.gene09172:CDS
TCAACTAGTCTAGGACTCCA+TGG 0.516380 4.3:+43635550 None:intergenic
ACACTTTCCGGCGAAACAAA+CGG 0.517832 4.3:+43636228 None:intergenic
AGGACGTGGCAATGAAAGAT+GGG 0.525036 4.3:-43636344 MS.gene09172:CDS
GCAACGGATTCAACTAGTCT+AGG 0.536222 4.3:+43635541 None:intergenic
CTCCATGGGGCCCACCCCAA+AGG 0.540950 4.3:+43635565 None:intergenic
TTTGTTTATGTCCTTTGGGG+TGG 0.541387 4.3:-43635576 MS.gene09172:CDS
ATCCTAAGAACGTTAGTGGA+AGG 0.545074 4.3:-43636128 MS.gene09172:CDS
CCTGTGCTACGGTTCCAGAA+AGG 0.547303 4.3:+43636530 None:intergenic
GCATTATTCAAAGAAACTTG+TGG 0.550200 4.3:-43635369 MS.gene09172:CDS
ACTTGTGGGACCCACGTACA+AGG 0.550385 4.3:-43635354 MS.gene09172:CDS
AGTTTCTTTGAATAATGCTA+AGG 0.554463 4.3:+43635374 None:intergenic
CAACTAGTCTAGGACTCCAT+GGG 0.554729 4.3:+43635551 None:intergenic
CTTTGAGTGAAAAGGTGGAT+AGG 0.557127 4.3:-43635270 MS.gene09172:CDS
CATGGGAAGCTACGAAAACA+TGG 0.561296 4.3:+43636501 None:intergenic
CCTTTCTGGAACCGTAGCAC+AGG 0.563639 4.3:-43636530 MS.gene09172:CDS
CGTTGCATTAGGTTGTGTGC+CGG 0.564884 4.3:-43635524 MS.gene09172:CDS
TGGCGGAGAAAGATGTTTGG+AGG 0.571787 4.3:-43635432 MS.gene09172:CDS
ACATTCTTTGAGTGAAAAGG+TGG 0.573238 4.3:-43635275 MS.gene09172:CDS
GTCTCCGTAGGTAAAACCTC+CGG 0.581628 4.3:+43635641 None:intergenic
ACATGTTCAACTTTCTGACA+TGG 0.583329 4.3:+43636261 None:intergenic
AAAACAAGAACCGAAATCAT+GGG 0.585303 4.3:+43636484 None:intergenic
TAGGTCTTATAGGAGTTCAA+GGG 0.590547 4.3:-43635251 MS.gene09172:CDS
CCGAACGTGCAATTTCTGAC+TGG 0.596924 4.3:+43635602 None:intergenic
GGAGATTTCAGTCACGGTGG+CGG 0.597754 4.3:-43635449 MS.gene09172:CDS
TTTCTTTCGTGGGAAAATGG+AGG 0.599457 4.3:-43636154 MS.gene09172:CDS
CGGCACACAACCTAATGCAA+CGG 0.603395 4.3:+43635525 None:intergenic
GTAGTGCTTTCCTTGTACGT+GGG 0.605083 4.3:+43635344 None:intergenic
ACGGTTCCAGAAAGGGTACT+CGG 0.610843 4.3:+43636538 None:intergenic
TTCAATAGTGGGGTCCACGA+AGG 0.617215 4.3:-43635319 MS.gene09172:CDS
AGTAGTGCTTTCCTTGTACG+TGG 0.619416 4.3:+43635343 None:intergenic
GCCACCGTGACTGAAATCTC+CGG 0.620485 4.3:+43635451 None:intergenic
CGGCAAACCTATGTCTCCGT+AGG 0.630204 4.3:+43635629 None:intergenic
CATGGTCGGATCCTGTGCTA+CGG 0.632492 4.3:+43636519 None:intergenic
CTGGCCGGAGATTTCAGTCA+CGG 0.634104 4.3:-43635455 MS.gene09172:CDS
AACGTGCATGACCTATTGCT+GGG 0.647771 4.3:+43636594 None:intergenic
GGAAGCTACGAAAACATGGT+CGG 0.655221 4.3:+43636505 None:intergenic
ATGAAACTCATGCATAAACA+TGG 0.655722 4.3:-43637088 MS.gene09172:intron
AAAATTTCAACAATGACCGG+AGG 0.658957 4.3:-43635657 MS.gene09172:CDS
GGACGTGGCAATGAAAGATG+GGG 0.696171 4.3:-43636343 MS.gene09172:CDS
GGAAAGTGTACGGAATACGA+TGG 0.698014 4.3:-43636214 MS.gene09172:CDS
GTGCCGGTAATCATAGCGGA+CGG 0.703809 4.3:-43635508 MS.gene09172:CDS
TCCACGAAGGAGATGCCACG+TGG 0.707958 4.3:-43635306 MS.gene09172:CDS
AGAATGTAGGACCTGCCACG+TGG 0.729796 4.3:+43635291 None:intergenic
GCCGGAGATTTCAGTCACGG+TGG 0.735933 4.3:-43635452 MS.gene09172:CDS
GTTCCAGAAAGGGTACTCGG+TGG 0.747263 4.3:+43636541 None:intergenic
AACTAGTCTAGGACTCCATG+GGG 0.754174 4.3:+43635552 None:intergenic
TTGTGTGCCGGTAATCATAG+CGG 0.757421 4.3:-43635512 MS.gene09172:CDS
AATCTCCGGCCAGTTCACGG+CGG 0.780614 4.3:+43635465 None:intergenic
CTTTCCGGCGAAACAAACGG+TGG 0.795710 4.3:+43636231 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGTAATATTTATGAAAA+TGG + chr4.3:43635275-43635294 None:intergenic 10.0%
!!! ATTTTCATAAATATTACAAA+TGG - chr4.3:43635274-43635293 MS.gene09172:CDS 10.0%
!!! TTTTTTTTTGTTTTGTTTTT+GGG - chr4.3:43635934-43635953 MS.gene09172:intron 10.0%
!!! TTTTTTTTTTGTTTTGTTTT+TGG - chr4.3:43635933-43635952 MS.gene09172:intron 10.0%
!! ATATGAGACAAAATTAATAT+AGG - chr4.3:43636394-43636413 MS.gene09172:intron 15.0%
!! CAATAAAAAGTAATATTTGT+TGG - chr4.3:43636338-43636357 MS.gene09172:CDS 15.0%
!! TAATATGAAAATTAGTTCAT+TGG + chr4.3:43636442-43636461 None:intergenic 15.0%
!!! AATTAAGATTTTAATTAGAG+AGG + chr4.3:43636241-43636260 None:intergenic 15.0%
!!! TATTGTTTTTTTTATGGATA+AGG + chr4.3:43635442-43635461 None:intergenic 15.0%
!!! TGATGATATTGTTTTTTTTA+TGG + chr4.3:43635448-43635467 None:intergenic 15.0%
!! ATTGAATTCTTCATAATTTC+AGG + chr4.3:43635995-43636014 None:intergenic 20.0%
!!! AATATTACAAATGGATTCTT+TGG - chr4.3:43635283-43635302 MS.gene09172:CDS 20.0%
!!! AGAAAGTGATTTTATGATAT+GGG + chr4.3:43635415-43635434 None:intergenic 20.0%
!!! ATGTGATTATTGAGTTTTTT+TGG - chr4.3:43636557-43636576 MS.gene09172:CDS 20.0%
!!! TGGTTTTTTTTTTTTTACCA+GGG + chr4.3:43635856-43635875 None:intergenic 20.0%
!!! TTGGTTTTTTTTTTTTTACC+AGG + chr4.3:43635857-43635876 None:intergenic 20.0%
! AAGAAAAAATCAGCTTCATA+AGG + chr4.3:43635651-43635670 None:intergenic 25.0%
! AGAAAAAATCAGCTTCATAA+GGG + chr4.3:43635650-43635669 None:intergenic 25.0%
! AGTTTCTTTGAATAATGCTA+AGG + chr4.3:43636939-43636958 None:intergenic 25.0%
! CATTATTCAAAGAAACTTGT+GGG - chr4.3:43636942-43636961 MS.gene09172:intron 25.0%
! TTTATTGTGAGAGACAAATT+GGG + chr4.3:43636325-43636344 None:intergenic 25.0%
!! AATAAATGAGTTTTGCATCT+TGG + chr4.3:43635570-43635589 None:intergenic 25.0%
!! AATTTATGTTTTGCCTTTGA+AGG - chr4.3:43636608-43636627 MS.gene09172:CDS 25.0%
!! ATAAATGAGTTTTGCATCTT+GGG + chr4.3:43635569-43635588 None:intergenic 25.0%
!! GAGAAAGTGATTTTATGATA+TGG + chr4.3:43635416-43635435 None:intergenic 25.0%
!! TTATGTTATTGTTGTTGTTG+TGG + chr4.3:43635368-43635387 None:intergenic 25.0%
!! TTTTATTGTGAGAGACAAAT+TGG + chr4.3:43636326-43636345 None:intergenic 25.0%
!!! TTTCAAGTTCTTCAAATTAG+TGG + chr4.3:43635487-43635506 None:intergenic 25.0%
AAAACAAGAACCGAAATCAT+GGG + chr4.3:43635829-43635848 None:intergenic 30.0%
AAGTTACAAGAAACGTAAAC+TGG + chr4.3:43635678-43635697 None:intergenic 30.0%
ATGAAACTCATGCATAAACA+TGG - chr4.3:43635222-43635241 MS.gene09172:CDS 30.0%
CACCAAAATACAACAAAAAC+TGG - chr4.3:43635535-43635554 MS.gene09172:CDS 30.0%
GCATTATTCAAAGAAACTTG+TGG - chr4.3:43636941-43636960 MS.gene09172:intron 30.0%
GGAAAAATTTCAACAATGAC+CGG - chr4.3:43636650-43636669 MS.gene09172:intron 30.0%
TCAATTGTGTTACAAACGTT+TGG - chr4.3:43636010-43636029 MS.gene09172:intron 30.0%
! GGCTAGTTGACAAAAAAAAT+TGG + chr4.3:43636421-43636440 None:intergenic 30.0%
!! AAAGCACTACTTTTCAATAG+TGG - chr4.3:43636979-43636998 MS.gene09172:intron 30.0%
!! AAGCACTACTTTTCAATAGT+GGG - chr4.3:43636980-43636999 MS.gene09172:intron 30.0%
!! AGCCAGTTTTTGTTGTATTT+TGG + chr4.3:43635540-43635559 None:intergenic 30.0%
!! ATATTTGGGTTTTCTTTCGT+GGG - chr4.3:43636146-43636165 MS.gene09172:CDS 30.0%
!! CAGTTTTTGTTGTATTTTGG+TGG + chr4.3:43635537-43635556 None:intergenic 30.0%
!! TTAGTGGTTTGAAGTTTTTG+AGG + chr4.3:43635471-43635490 None:intergenic 30.0%
!!! GTGTTTTGTTTATGTCCTTT+GGG - chr4.3:43636730-43636749 MS.gene09172:intron 30.0%
!!! GTTTTGTTTTTGGGTTTTGA+AGG - chr4.3:43635943-43635962 MS.gene09172:intron 30.0%
!!! TGGTTTTTTTCTTGTTGTTG+AGG + chr4.3:43635255-43635274 None:intergenic 30.0%
!!! TGTTTTGTTTATGTCCTTTG+GGG - chr4.3:43636731-43636750 MS.gene09172:intron 30.0%
AAAATTTCAACAATGACCGG+AGG - chr4.3:43636653-43636672 MS.gene09172:intron 35.0%
ACATGTTCAACTTTCTGACA+TGG + chr4.3:43636052-43636071 None:intergenic 35.0%
ACATTCTTTGAGTGAAAAGG+TGG - chr4.3:43637035-43637054 MS.gene09172:intron 35.0%
ACCATAGCCAAATTAGTTCA+TGG + chr4.3:43636502-43636521 None:intergenic 35.0%
CATTTGTAACATGAAGCTGT+TGG + chr4.3:43635876-43635895 None:intergenic 35.0%
CCATAGCCAAATTAGTTCAT+GGG + chr4.3:43636501-43636520 None:intergenic 35.0%
CCTACATTCTTTGAGTGAAA+AGG - chr4.3:43637032-43637051 MS.gene09172:intron 35.0%
GAAAACAAGAACCGAAATCA+TGG + chr4.3:43635830-43635849 None:intergenic 35.0%
GGGTGAGATATTAGAAAAAG+TGG - chr4.3:43636903-43636922 MS.gene09172:intron 35.0%
! AAAAGGTGGATAGGTCTTAT+AGG - chr4.3:43637049-43637068 MS.gene09172:intron 35.0%
! ATAGGTCTTATAGGAGTTCA+AGG - chr4.3:43637058-43637077 MS.gene09172:intron 35.0%
! CCTTTTCACTCAAAGAATGT+AGG + chr4.3:43637035-43637054 None:intergenic 35.0%
! GAATACGATGGAGAATTTTC+CGG - chr4.3:43636108-43636127 MS.gene09172:intron 35.0%
! TAGGTCTTATAGGAGTTCAA+GGG - chr4.3:43637059-43637078 MS.gene09172:intron 35.0%
!! AGCACTACTTTTCAATAGTG+GGG - chr4.3:43636981-43637000 MS.gene09172:intron 35.0%
!! GATATTTGGGTTTTCTTTCG+TGG - chr4.3:43636145-43636164 MS.gene09172:CDS 35.0%
!!! GGTGTTTTGTTTATGTCCTT+TGG - chr4.3:43636729-43636748 MS.gene09172:intron 35.0%
!!! TTGTTTTTGGGTTTTGAAGG+AGG - chr4.3:43635946-43635965 MS.gene09172:intron 35.0%
AACCTTCCACTAACGTTCTT+AGG + chr4.3:43636187-43636206 None:intergenic 40.0%
ATCCTAAGAACGTTAGTGGA+AGG - chr4.3:43636182-43636201 MS.gene09172:CDS 40.0%
ATCGTATTCCGTACACTTTC+CGG + chr4.3:43636097-43636116 None:intergenic 40.0%
CACTGACCCATGAACTAATT+TGG - chr4.3:43636492-43636511 MS.gene09172:CDS 40.0%
CCCATGAACTAATTTGGCTA+TGG - chr4.3:43636498-43636517 MS.gene09172:CDS 40.0%
CTAGTTGAATCCGTTGCATT+AGG - chr4.3:43636775-43636794 MS.gene09172:intron 40.0%
CTGTTCTCACTTTCCTTCAA+AGG + chr4.3:43636624-43636643 None:intergenic 40.0%
CTTTGAGTGAAAAGGTGGAT+AGG - chr4.3:43637040-43637059 MS.gene09172:intron 40.0%
GGAAAGTGAGAACAGTGATA+TGG - chr4.3:43636629-43636648 MS.gene09172:CDS 40.0%
GTTCATCCTAAGAACGTTAG+TGG - chr4.3:43636178-43636197 MS.gene09172:CDS 40.0%
TATTGCTGGGAAGCCATTAA+CGG + chr4.3:43635706-43635725 None:intergenic 40.0%
TGCAATTTCTGACTGGTAAC+CGG + chr4.3:43636704-43636723 None:intergenic 40.0%
TGTAACTTCAGCACCGTTAA+TGG - chr4.3:43635690-43635709 MS.gene09172:intron 40.0%
TTCGTAGCTTCCCATGATTT+CGG - chr4.3:43635816-43635835 MS.gene09172:intron 40.0%
TTTCTTTCGTGGGAAAATGG+AGG - chr4.3:43636156-43636175 MS.gene09172:CDS 40.0%
! ATGGAGAATTTTCCGGTGAA+CGG - chr4.3:43636115-43636134 MS.gene09172:intron 40.0%
! GGTTTTCTTTCGTGGGAAAA+TGG - chr4.3:43636153-43636172 MS.gene09172:CDS 40.0%
! GTGAGAGACAAATTGGGTTT+TGG + chr4.3:43636319-43636338 None:intergenic 40.0%
! TTTGTTTATGTCCTTTGGGG+TGG - chr4.3:43636734-43636753 MS.gene09172:intron 40.0%
!! TTGTTTATGTCCTTTGGGGT+GGG - chr4.3:43636735-43636754 MS.gene09172:intron 40.0%
AACGTGCATGACCTATTGCT+GGG + chr4.3:43635719-43635738 None:intergenic 45.0%
ACACTTTCCGGCGAAACAAA+CGG + chr4.3:43636085-43636104 None:intergenic 45.0%
AGGACGTGGCAATGAAAGAT+GGG - chr4.3:43635966-43635985 MS.gene09172:intron 45.0%
AGTAGTGCTTTCCTTGTACG+TGG + chr4.3:43636970-43636989 None:intergenic 45.0%
ATACCGTCCGCTATGATTAC+CGG + chr4.3:43636808-43636827 None:intergenic 45.0%
CATGGGAAGCTACGAAAACA+TGG + chr4.3:43635812-43635831 None:intergenic 45.0%
CCAGTCAGAAATTGCACGTT+CGG - chr4.3:43636708-43636727 MS.gene09172:intron 45.0%
GAGAAAGATGTTTGGAGGCT+GGG - chr4.3:43636883-43636902 MS.gene09172:intron 45.0%
GGAAGCTACGAAAACATGGT+CGG + chr4.3:43635808-43635827 None:intergenic 45.0%
GTAGTGCTTTCCTTGTACGT+GGG + chr4.3:43636969-43636988 None:intergenic 45.0%
GTTAATGGCTTCCCAGCAAT+AGG - chr4.3:43635705-43635724 MS.gene09172:intron 45.0%
! AACTAGTCTAGGACTCCATG+GGG + chr4.3:43636761-43636780 None:intergenic 45.0%
! AGAATTTTCCGGTGAACGGA+CGG - chr4.3:43636119-43636138 MS.gene09172:CDS 45.0%
! ATGACCGGAGGTTTTACCTA+CGG - chr4.3:43636665-43636684 MS.gene09172:intron 45.0%
! CAACTAGTCTAGGACTCCAT+GGG + chr4.3:43636762-43636781 None:intergenic 45.0%
! GCAACGGATTCAACTAGTCT+AGG + chr4.3:43636772-43636791 None:intergenic 45.0%
! GGAAAGTGTACGGAATACGA+TGG - chr4.3:43636096-43636115 MS.gene09172:intron 45.0%
! GGTTTTACCTACGGAGACAT+AGG - chr4.3:43636674-43636693 MS.gene09172:intron 45.0%
! TCAACTAGTCTAGGACTCCA+TGG + chr4.3:43636763-43636782 None:intergenic 45.0%
! TTGTGTGCCGGTAATCATAG+CGG - chr4.3:43636798-43636817 MS.gene09172:intron 45.0%
! TTGTTTCGCCGGAAAGTGTA+CGG - chr4.3:43636086-43636105 MS.gene09172:intron 45.0%
!! TAATCATAGCGGACGGTATC+CGG - chr4.3:43636809-43636828 MS.gene09172:intron 45.0%
!!! TCTTAATTTTTTATTTTTTT+TGG - chr4.3:43636253-43636272 MS.gene09172:CDS 5.0%
CCGAACGTGCAATTTCTGAC+TGG + chr4.3:43636711-43636730 None:intergenic 50.0%
CGGCACACAACCTAATGCAA+CGG + chr4.3:43636788-43636807 None:intergenic 50.0%
CTACGGAGACATAGGTTTGC+CGG - chr4.3:43636682-43636701 MS.gene09172:intron 50.0%
CTGTGCTACGGTTCCAGAAA+GGG + chr4.3:43635782-43635801 None:intergenic 50.0%
GAACGGACGGCGAGATATTT+GGG - chr4.3:43636132-43636151 MS.gene09172:CDS 50.0%
GAACGTGCATGACCTATTGC+TGG + chr4.3:43635720-43635739 None:intergenic 50.0%
GAGGACGTGGCAATGAAAGA+TGG - chr4.3:43635965-43635984 MS.gene09172:intron 50.0%
GATACCACCGTTTGTTTCGC+CGG - chr4.3:43636075-43636094 MS.gene09172:intron 50.0%
GGACGTGGCAATGAAAGATG+GGG - chr4.3:43635967-43635986 MS.gene09172:intron 50.0%
GGAGAAAGATGTTTGGAGGC+TGG - chr4.3:43636882-43636901 MS.gene09172:intron 50.0%
GTCTCCGTAGGTAAAACCTC+CGG + chr4.3:43636672-43636691 None:intergenic 50.0%
TGAAATCTCCGGCCAGTTCA+CGG + chr4.3:43636851-43636870 None:intergenic 50.0%
TGAACGGACGGCGAGATATT+TGG - chr4.3:43636131-43636150 MS.gene09172:CDS 50.0%
TGGCGGAGAAAGATGTTTGG+AGG - chr4.3:43636878-43636897 MS.gene09172:intron 50.0%
! CGTTGCATTAGGTTGTGTGC+CGG - chr4.3:43636786-43636805 MS.gene09172:intron 50.0%
! TTCAATAGTGGGGTCCACGA+AGG - chr4.3:43636991-43637010 MS.gene09172:intron 50.0%
!! ACGGTTCCAGAAAGGGTACT+CGG + chr4.3:43635775-43635794 None:intergenic 50.0%
!!! CGGTTCTTGTTTTCACACCC+TGG - chr4.3:43635836-43635855 MS.gene09172:intron 50.0%
!!! TTGGGTTTTGAAGGAGGACG+TGG - chr4.3:43635952-43635971 MS.gene09172:intron 50.0%
ACTTGTGGGACCCACGTACA+AGG - chr4.3:43636956-43636975 MS.gene09172:intron 55.0%
AGAATGTAGGACCTGCCACG+TGG + chr4.3:43637022-43637041 None:intergenic 55.0%
ATATCTCGCCGTCCGTTCAC+CGG + chr4.3:43636130-43636149 None:intergenic 55.0%
CAGTTCACGGCGGAGGAAAA+CGG + chr4.3:43636838-43636857 None:intergenic 55.0%
CATGGTCGGATCCTGTGCTA+CGG + chr4.3:43635794-43635813 None:intergenic 55.0%
CCTGTGCTACGGTTCCAGAA+AGG + chr4.3:43635783-43635802 None:intergenic 55.0%
CGGCAAACCTATGTCTCCGT+AGG + chr4.3:43636684-43636703 None:intergenic 55.0%
CTGGCCGGAGATTTCAGTCA+CGG - chr4.3:43636855-43636874 MS.gene09172:intron 55.0%
CTTTCCGGCGAAACAAACGG+TGG + chr4.3:43636082-43636101 None:intergenic 55.0%
GCCACCGTGACTGAAATCTC+CGG + chr4.3:43636862-43636881 None:intergenic 55.0%
GGAGATTTCAGTCACGGTGG+CGG - chr4.3:43636861-43636880 MS.gene09172:intron 55.0%
TCTCCACCGAGTACCCTTTC+TGG - chr4.3:43635766-43635785 MS.gene09172:intron 55.0%
! GTGCCGGTAATCATAGCGGA+CGG - chr4.3:43636802-43636821 MS.gene09172:intron 55.0%
!! CCTTTCTGGAACCGTAGCAC+AGG - chr4.3:43635780-43635799 MS.gene09172:intron 55.0%
!! CGGTGGCGGAGAAAGATGTT+TGG - chr4.3:43636875-43636894 MS.gene09172:intron 55.0%
!! GTTCCAGAAAGGGTACTCGG+TGG + chr4.3:43635772-43635791 None:intergenic 55.0%
AATCTCCGGCCAGTTCACGG+CGG + chr4.3:43636848-43636867 None:intergenic 60.0%
CGGCGGAGGAAAACGGTAAC+CGG + chr4.3:43636831-43636850 None:intergenic 60.0%
GCCGGAGATTTCAGTCACGG+TGG - chr4.3:43636858-43636877 MS.gene09172:intron 60.0%
TCCACGAAGGAGATGCCACG+TGG - chr4.3:43637004-43637023 MS.gene09172:intron 60.0%
! CGTTTTCCTCCGCCGTGAAC+TGG - chr4.3:43636836-43636855 MS.gene09172:intron 60.0%
CGAAGGAGATGCCACGTGGC+AGG - chr4.3:43637008-43637027 MS.gene09172:intron 65.0%
GCCACGTGGCATCTCCTTCG+TGG + chr4.3:43637008-43637027 None:intergenic 65.0%
TTCCTCCGCCGTGAACTGGC+CGG - chr4.3:43636840-43636859 MS.gene09172:intron 65.0%
CTCCATGGGGCCCACCCCAA+AGG + chr4.3:43636748-43636767 None:intergenic 70.0%
!! GTCCTTTGGGGTGGGCCCCA+TGG - chr4.3:43636743-43636762 MS.gene09172:intron 70.0%
CTCCGGCCAGTTCACGGCGG+AGG + chr4.3:43636845-43636864 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 43635222 43637110 43635222 ID=MS.gene09172
chr4.3 mRNA 43635222 43637110 43635222 ID=MS.gene09172.t1;Parent=MS.gene09172
chr4.3 exon 43637089 43637110 43637089 ID=MS.gene09172.t1.exon1;Parent=MS.gene09172.t1
chr4.3 CDS 43637089 43637110 43637089 ID=cds.MS.gene09172.t1;Parent=MS.gene09172.t1
chr4.3 exon 43636475 43636653 43636475 ID=MS.gene09172.t1.exon2;Parent=MS.gene09172.t1
chr4.3 CDS 43636475 43636653 43636475 ID=cds.MS.gene09172.t1;Parent=MS.gene09172.t1
chr4.3 exon 43636117 43636367 43636117 ID=MS.gene09172.t1.exon3;Parent=MS.gene09172.t1
chr4.3 CDS 43636117 43636367 43636117 ID=cds.MS.gene09172.t1;Parent=MS.gene09172.t1
chr4.3 exon 43635222 43635702 43635222 ID=MS.gene09172.t1.exon4;Parent=MS.gene09172.t1
chr4.3 CDS 43635222 43635702 43635222 ID=cds.MS.gene09172.t1;Parent=MS.gene09172.t1
Gene Sequence

>MS.gene09172

ATGAAACTCATGCATAAACATGGTATTAGACCTCAACAACAAGAAAAAAACCATTTTCATAAATATTACAAATGGATTCTTTGGCTTTCTCTTTCTCTCTATTTCTTCACTTCTTATCTCATCAGCAACAACAACAATCACAACCACAACAACAACAATAACATAAAACATCATCAAATAACATCAACAACCCATATCATAAAATCACTTTCTCAATCCTTATCCATAAAAAAAACAATATCATCACCTCAAAAACTTCAAACCACTAATTTGAAGAACTTGAAAAACTTGAAAATATTTGTGTATGATCTTCCACCAAAATACAACAAAAACTGGCTTAAAAACCCAAGATGCAAAACTCATTTATTTGCTTCAGAAGTTGCTATTCATAGAGCTTTATTAACAAGTGATGTTAGAACTTTTGACCCTTATGAAGCTGATTTTTTCTTTGTACCAGTTTACGTTTCTTGTAACTTCAGCACCGTTAATGGCTTCCCAGCAATAGGTCATGCACGTTCATTGATTTCTTCAGCAGTGAAACTCATCTCCACCGAGTACCCTTTCTGGAACCGTAGCACAGGATCCGACCATGTTTTCGTAGCTTCCCATGATTTCGGTTCTTGTTTTCACACCCTGGTAAAAAAAAAAAAACCAACAGCTTCATGTTACAAATGTTAGAATTTTTTTTTTTTTTGAGTTTTTCGAATGATGTTTTTTTTTTGTTTTGTTTTTGGGTTTTGAAGGAGGACGTGGCAATGAAAGATGGGGTACCTGAAATTATGAAGAATTCAATTGTGTTACAAACGTTTGGTGTTACATATGATCATCCATGTCAGAAAGTTGAACATGTTGTGATACCACCGTTTGTTTCGCCGGAAAGTGTACGGAATACGATGGAGAATTTTCCGGTGAACGGACGGCGAGATATTTGGGTTTTCTTTCGTGGGAAAATGGAGGTTCATCCTAAGAACGTTAGTGGAAGGTTTTACAGCAAGTAATCACACTTTAACTTTTAACCTCTCTAATTAAAATCTTAATTTTTTATTTTTTTTGGTACATAAAATCTTAATTTATATGCTTATTATTTTAAAAGACCAAAACCCAATTTGTCTCTCACAATAAAAAGTAATATTTGTTGGTAAAAATAAAAAACGTAATATTTATACATTTTTATATGAGACAAAATTAATATAGGACCAATTTTTTTTGTCAACTAGCCAATGAACTAATTTTCATATTAGAGAGTAGAGACAAATTATTTCATTTATTACACTGACCCATGAACTAATTTGGCTATGGTATAAAAATGTTTAAGTTTTATTTTTTAAGATATAAATGTGATTATTGAGTTTTTTTGGTTATTATTGTATTAGTGATTTTCTAATGAATTTATGTTTTGCCTTTGAAGGAAAGTGAGAACAGTGATATGGAAAAATTTCAACAATGACCGGAGGTTTTACCTACGGAGACATAGGTTTGCCGGTTACCAGTCAGAAATTGCACGTTCGGTGTTTTGTTTATGTCCTTTGGGGTGGGCCCCATGGAGTCCTAGACTAGTTGAATCCGTTGCATTAGGTTGTGTGCCGGTAATCATAGCGGACGGTATCCGGTTACCGTTTTCCTCCGCCGTGAACTGGCCGGAGATTTCAGTCACGGTGGCGGAGAAAGATGTTTGGAGGCTGGGTGAGATATTAGAAAAAGTGGCTGCTACAAACCTTAGCATTATTCAAAGAAACTTGTGGGACCCACGTACAAGGAAAGCACTACTTTTCAATAGTGGGGTCCACGAAGGAGATGCCACGTGGCAGGTCCTACATTCTTTGAGTGAAAAGGTGGATAGGTCTTATAGGAGTTCAAGGGTTTCGCGCCAATTACAATTTGACACGTAA
Protein sequence

>MS.gene09172.t1

MKLMHKHVYVSCNFSTVNGFPAIGHARSLISSAVKLISTEYPFWNRSTGSDHVFVASHDFGSCFHTLEDVAMKDGVPEIMKNSIVLQTFGVTYDHPCQKVEHVVIPPFVSPESVRNTMENFPVNGRRDIWVFFRGKMEVHPKNVSGRFYSKKVRTVIWKNFNNDRRFYLRRHRFAGYQSEIARSVFCLCPLGWAPWSPRLVESVALGCVPVIIADGIRLPFSSAVNWPEISVTVAEKDVWRLGEILEKVAATNLSIIQRNLWDPRTRKALLFNSGVHEGDATWQVLHSLSEKVDRSYRSSRVSRQLQFDT