Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09317.t1 | XP_013451947.1 | 93.8 | 160 | 10 | 0 | 1 | 160 | 156 | 315 | 2.60E-85 | 324.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09317.t1 | Q9SZ83 | 66.0 | 159 | 54 | 0 | 1 | 159 | 156 | 314 | 3.6e-59 | 229.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09317.t1 | A0A072UJH2 | 93.8 | 160 | 10 | 0 | 1 | 160 | 156 | 315 | 1.9e-85 | 324.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052902 | MS.gene09317 | 0.831122 | 2.00E-55 | -1.69E-46 |
| MS.gene053356 | MS.gene09317 | 0.80309 | 4.11E-49 | -1.69E-46 |
| MS.gene05417 | MS.gene09317 | 0.805285 | 1.44E-49 | -1.69E-46 |
| MS.gene057750 | MS.gene09317 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene09317 | 0.809179 | 2.15E-50 | -1.69E-46 |
| MS.gene059124 | MS.gene09317 | 0.851398 | 9.11E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09317.t1 | MTR_6g038640 | 93.750 | 160 | 10 | 0 | 1 | 160 | 156 | 315 | 1.20e-112 | 325 |
| MS.gene09317.t1 | MTR_6g038650 | 88.125 | 160 | 14 | 2 | 1 | 160 | 156 | 310 | 4.98e-100 | 292 |
| MS.gene09317.t1 | MTR_1g097250 | 73.585 | 159 | 42 | 0 | 1 | 159 | 155 | 313 | 1.06e-85 | 256 |
| MS.gene09317.t1 | MTR_6g038350 | 62.893 | 159 | 59 | 0 | 1 | 159 | 158 | 316 | 3.01e-72 | 222 |
| MS.gene09317.t1 | MTR_6g038370 | 62.893 | 159 | 59 | 0 | 1 | 159 | 158 | 316 | 3.01e-72 | 222 |
| MS.gene09317.t1 | MTR_6g038390 | 59.119 | 159 | 65 | 0 | 1 | 159 | 158 | 316 | 6.85e-68 | 211 |
| MS.gene09317.t1 | MTR_6g066230 | 49.693 | 163 | 77 | 2 | 1 | 159 | 156 | 317 | 1.50e-53 | 174 |
| MS.gene09317.t1 | MTR_3g064900 | 48.447 | 161 | 81 | 1 | 1 | 159 | 158 | 318 | 2.22e-46 | 155 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09317.t1 | AT4G09670 | 66.038 | 159 | 54 | 0 | 1 | 159 | 156 | 314 | 7.27e-75 | 229 |
| MS.gene09317.t1 | AT1G34200 | 54.717 | 159 | 61 | 1 | 1 | 159 | 157 | 304 | 2.66e-55 | 178 |
| MS.gene09317.t1 | AT1G66130 | 36.486 | 148 | 84 | 5 | 21 | 160 | 175 | 320 | 2.50e-22 | 92.4 |
Find 34 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCGGTGCTTCTTTATAC+TGG | 0.248239 | 6.2:-33669922 | MS.gene09317:CDS |
| CTCGACGCTCTTGGCTCTCT+TGG | 0.311575 | 6.2:-33670058 | MS.gene09317:CDS |
| CAGCAGTTATATCCATTGTT+AGG | 0.316283 | 6.2:+33669855 | None:intergenic |
| TTGATGATCTTGTCACTAGA+TGG | 0.323932 | 6.2:-33669742 | MS.gene09317:CDS |
| TCTCCCTCAGGAGGCTCTTA+TGG | 0.328360 | 6.2:-33669684 | MS.gene09317:CDS |
| ACGAAGGATGGTATTGTGTT+AGG | 0.339462 | 6.2:-33670033 | MS.gene09317:CDS |
| ATTCACACCTGCTTTACATT+TGG | 0.392125 | 6.2:-33670127 | MS.gene09317:CDS |
| GAGCCAAGAGCGTCGAGATC+CGG | 0.396802 | 6.2:+33670064 | None:intergenic |
| ATAACTGCTGTTGGGACAAA+AGG | 0.403721 | 6.2:-33669842 | MS.gene09317:CDS |
| AAGCCGGATCTCGACGCTCT+TGG | 0.416131 | 6.2:-33670067 | MS.gene09317:CDS |
| TGATGATCTTGTCACTAGAT+GGG | 0.427776 | 6.2:-33669741 | MS.gene09317:CDS |
| CGAAGGATGGTATTGTGTTA+GGG | 0.448127 | 6.2:-33670032 | MS.gene09317:CDS |
| GCTTCTTTATACTGGGAAGA+TGG | 0.493899 | 6.2:-33669914 | MS.gene09317:CDS |
| ATCAGCACCAAATGTAAAGC+AGG | 0.503357 | 6.2:+33670120 | None:intergenic |
| AAATGACATCCGCGTGAAGC+CGG | 0.507297 | 6.2:-33670083 | MS.gene09317:CDS |
| GATGATCTTGTCACTAGATG+GGG | 0.507725 | 6.2:-33669740 | MS.gene09317:CDS |
| ACAATGGATATAACTGCTGT+TGG | 0.509647 | 6.2:-33669851 | MS.gene09317:CDS |
| CAATGGATATAACTGCTGTT+GGG | 0.519696 | 6.2:-33669850 | MS.gene09317:CDS |
| TTGTGTTAGGGCAATTCTGT+GGG | 0.521428 | 6.2:-33670020 | MS.gene09317:CDS |
| GCTCTCTTGGTGACGAAGGA+TGG | 0.525086 | 6.2:-33670045 | MS.gene09317:CDS |
| ACTCCTGCTTTATTAAGCAC+TGG | 0.538111 | 6.2:+33669953 | None:intergenic |
| TTGCGGTGCTTCTTTATACT+GGG | 0.542159 | 6.2:-33669921 | MS.gene09317:CDS |
| ATTGTGTTAGGGCAATTCTG+TGG | 0.545531 | 6.2:-33670021 | MS.gene09317:CDS |
| TTTCTTATCAAACCTAACAA+TGG | 0.550927 | 6.2:-33669867 | MS.gene09317:CDS |
| AATAGAAAACGATCTCCCTC+AGG | 0.553606 | 6.2:-33669696 | MS.gene09317:CDS |
| ACGTGAGTATAAGTGCTGTT+TGG | 0.556124 | 6.2:-33669624 | MS.gene09317:CDS |
| CTTGGCTCTCTTGGTGACGA+AGG | 0.568618 | 6.2:-33670049 | MS.gene09317:CDS |
| GAGCCAGTGCTTAATAAAGC+AGG | 0.571428 | 6.2:-33669956 | MS.gene09317:CDS |
| ATTAAGCACTGGCTCGCGCG+TGG | 0.597320 | 6.2:+33669964 | None:intergenic |
| CGTCGAGATCCGGCTTCACG+CGG | 0.615029 | 6.2:+33670074 | None:intergenic |
| AGAAAACGATCTCCCTCAGG+AGG | 0.651262 | 6.2:-33669693 | MS.gene09317:CDS |
| TTACCATAAGAGCCTCCTGA+GGG | 0.661856 | 6.2:+33669681 | None:intergenic |
| GCAGGAGTGATTCTATCTTG+CGG | 0.669495 | 6.2:-33669938 | MS.gene09317:CDS |
| CTTACCATAAGAGCCTCCTG+AGG | 0.708450 | 6.2:+33669680 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTCTTATCAAACCTAACAA+TGG | - | chr6.2:33669879-33669898 | MS.gene09317:CDS | 25.0% |
| !! | TTTTCTATTATGTGCTTACT+CGG | + | chr6.2:33670039-33670058 | None:intergenic | 25.0% |
| CAACTACGAATTACCAAAAA+CGG | - | chr6.2:33669753-33669772 | MS.gene09317:CDS | 30.0% | |
| ! | ATGCTGTCTTTTCTTCATAA+GGG | + | chr6.2:33669957-33669976 | None:intergenic | 30.0% |
| !!! | ATGTCATTTTCGAGAAAATC+AGG | + | chr6.2:33669652-33669671 | None:intergenic | 30.0% |
| ACAATGGATATAACTGCTGT+TGG | - | chr6.2:33669895-33669914 | MS.gene09317:CDS | 35.0% | |
| ATTCACACCTGCTTTACATT+TGG | - | chr6.2:33669619-33669638 | MS.gene09317:CDS | 35.0% | |
| CAATGGATATAACTGCTGTT+GGG | - | chr6.2:33669896-33669915 | MS.gene09317:CDS | 35.0% | |
| CAGCAGTTATATCCATTGTT+AGG | + | chr6.2:33669894-33669913 | None:intergenic | 35.0% | |
| TGATGATCTTGTCACTAGAT+GGG | - | chr6.2:33670005-33670024 | MS.gene09317:CDS | 35.0% | |
| ! | GATGCTGTCTTTTCTTCATA+AGG | + | chr6.2:33669958-33669977 | None:intergenic | 35.0% |
| ! | TTGATGATCTTGTCACTAGA+TGG | - | chr6.2:33670004-33670023 | MS.gene09317:CDS | 35.0% |
| AATAGAAAACGATCTCCCTC+AGG | - | chr6.2:33670050-33670069 | MS.gene09317:CDS | 40.0% | |
| ACGAAGGATGGTATTGTGTT+AGG | - | chr6.2:33669713-33669732 | MS.gene09317:CDS | 40.0% | |
| ATAACTGCTGTTGGGACAAA+AGG | - | chr6.2:33669904-33669923 | MS.gene09317:CDS | 40.0% | |
| ATCAGCACCAAATGTAAAGC+AGG | + | chr6.2:33669629-33669648 | None:intergenic | 40.0% | |
| ATTGTGTTAGGGCAATTCTG+TGG | - | chr6.2:33669725-33669744 | MS.gene09317:CDS | 40.0% | |
| CGAAGGATGGTATTGTGTTA+GGG | - | chr6.2:33669714-33669733 | MS.gene09317:CDS | 40.0% | |
| GATGATCTTGTCACTAGATG+GGG | - | chr6.2:33670006-33670025 | MS.gene09317:CDS | 40.0% | |
| GCTTCTTTATACTGGGAAGA+TGG | - | chr6.2:33669832-33669851 | MS.gene09317:CDS | 40.0% | |
| TTGCGGTGCTTCTTTATACT+GGG | - | chr6.2:33669825-33669844 | MS.gene09317:CDS | 40.0% | |
| TTGTGTTAGGGCAATTCTGT+GGG | - | chr6.2:33669726-33669745 | MS.gene09317:CDS | 40.0% | |
| ! | ACGTGAGTATAAGTGCTGTT+TGG | - | chr6.2:33670122-33670141 | MS.gene09317:CDS | 40.0% |
| ! | ACTCCTGCTTTATTAAGCAC+TGG | + | chr6.2:33669796-33669815 | None:intergenic | 40.0% |
| CTTGCGGTGCTTCTTTATAC+TGG | - | chr6.2:33669824-33669843 | MS.gene09317:CDS | 45.0% | |
| GAGCCAGTGCTTAATAAAGC+AGG | - | chr6.2:33669790-33669809 | MS.gene09317:CDS | 45.0% | |
| GCAGGAGTGATTCTATCTTG+CGG | - | chr6.2:33669808-33669827 | MS.gene09317:CDS | 45.0% | |
| TTACCATAAGAGCCTCCTGA+GGG | + | chr6.2:33670068-33670087 | None:intergenic | 45.0% | |
| AAATGACATCCGCGTGAAGC+CGG | - | chr6.2:33669663-33669682 | MS.gene09317:CDS | 50.0% | |
| AGAAAACGATCTCCCTCAGG+AGG | - | chr6.2:33670053-33670072 | MS.gene09317:CDS | 50.0% | |
| CTTACCATAAGAGCCTCCTG+AGG | + | chr6.2:33670069-33670088 | None:intergenic | 50.0% | |
| ! | CGCGTGGCTAATACCGTTTT+TGG | + | chr6.2:33669769-33669788 | None:intergenic | 50.0% |
| TCTCCCTCAGGAGGCTCTTA+TGG | - | chr6.2:33670062-33670081 | MS.gene09317:CDS | 55.0% | |
| !! | CTTGGCTCTCTTGGTGACGA+AGG | - | chr6.2:33669697-33669716 | MS.gene09317:CDS | 55.0% |
| !! | GCTCTCTTGGTGACGAAGGA+TGG | - | chr6.2:33669701-33669720 | MS.gene09317:CDS | 55.0% |
| AAGCCGGATCTCGACGCTCT+TGG | - | chr6.2:33669679-33669698 | MS.gene09317:CDS | 60.0% | |
| ! | CTCGACGCTCTTGGCTCTCT+TGG | - | chr6.2:33669688-33669707 | MS.gene09317:CDS | 60.0% |
| !! | ATTAAGCACTGGCTCGCGCG+TGG | + | chr6.2:33669785-33669804 | None:intergenic | 60.0% |
| !! | GAGCCAAGAGCGTCGAGATC+CGG | + | chr6.2:33669685-33669704 | None:intergenic | 60.0% |
| CGTCGAGATCCGGCTTCACG+CGG | + | chr6.2:33669675-33669694 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 33669619 | 33670149 | 33669619 | ID=MS.gene09317 |
| chr6.2 | mRNA | 33669619 | 33670149 | 33669619 | ID=MS.gene09317.t1;Parent=MS.gene09317 |
| chr6.2 | exon | 33669619 | 33670149 | 33669619 | ID=MS.gene09317.t1.exon1;Parent=MS.gene09317.t1 |
| chr6.2 | CDS | 33669619 | 33670149 | 33669619 | ID=cds.MS.gene09317.t1;Parent=MS.gene09317.t1 |
| Gene Sequence |
| Protein sequence |
