Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09726.t1 | XP_003611369.1 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 8.80E-297 | 1028.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09726.t1 | O23522 | 55.4 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 5.3e-157 | 555.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09726.t1 | G7JXH0 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 6.3e-297 | 1028.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050238 | MS.gene09726 | 0.817763 | 2.79E-52 | -1.69E-46 |
| MS.gene051823 | MS.gene09726 | 0.80847 | 3.05E-50 | -1.69E-46 |
| MS.gene052016 | MS.gene09726 | 0.834409 | 3.05E-56 | -1.69E-46 |
| MS.gene053620 | MS.gene09726 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene054178 | MS.gene09726 | 0.805555 | 1.26E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene09726 | 0.808179 | 3.52E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09726.t1 | MTR_5g013230 | 97.628 | 548 | 11 | 2 | 1 | 546 | 1 | 548 | 0.0 | 1110 |
| MS.gene09726.t1 | MTR_4g099510 | 65.766 | 555 | 137 | 9 | 6 | 544 | 4 | 521 | 0.0 | 715 |
| MS.gene09726.t1 | MTR_7g081620 | 47.165 | 388 | 177 | 8 | 129 | 492 | 65 | 448 | 1.57e-120 | 362 |
| MS.gene09726.t1 | MTR_7g081645 | 45.553 | 371 | 167 | 9 | 133 | 492 | 75 | 421 | 8.94e-110 | 334 |
| MS.gene09726.t1 | MTR_4g077070 | 38.281 | 384 | 201 | 7 | 128 | 493 | 95 | 460 | 3.97e-84 | 271 |
| MS.gene09726.t1 | MTR_7g096120 | 39.737 | 380 | 194 | 11 | 133 | 493 | 74 | 437 | 1.12e-81 | 264 |
| MS.gene09726.t1 | MTR_7g096140 | 39.211 | 380 | 196 | 11 | 133 | 493 | 74 | 437 | 5.42e-81 | 261 |
| MS.gene09726.t1 | MTR_2g015690 | 35.156 | 384 | 192 | 9 | 133 | 493 | 20 | 369 | 1.28e-63 | 214 |
| MS.gene09726.t1 | MTR_7g006400 | 32.683 | 410 | 215 | 13 | 109 | 500 | 80 | 446 | 4.17e-57 | 199 |
| MS.gene09726.t1 | MTR_4g087810 | 33.075 | 387 | 195 | 11 | 133 | 495 | 13 | 359 | 6.13e-52 | 182 |
| MS.gene09726.t1 | MTR_4g087830 | 33.333 | 387 | 194 | 12 | 133 | 495 | 4 | 350 | 2.82e-51 | 181 |
| MS.gene09726.t1 | MTR_4g080100 | 29.353 | 402 | 214 | 9 | 129 | 493 | 24 | 392 | 1.01e-48 | 175 |
| MS.gene09726.t1 | MTR_1995s0010 | 40.928 | 237 | 114 | 7 | 251 | 475 | 1 | 223 | 5.20e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09726.t1 | AT4G16820 | 55.367 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 0.0 | 562 |
| MS.gene09726.t1 | AT2G44810 | 50.136 | 369 | 170 | 7 | 133 | 494 | 86 | 447 | 5.25e-123 | 369 |
| MS.gene09726.t1 | AT2G44810 | 50.413 | 363 | 166 | 7 | 139 | 494 | 2 | 357 | 1.95e-120 | 359 |
| MS.gene09726.t1 | AT1G30370 | 38.015 | 413 | 219 | 10 | 124 | 517 | 105 | 499 | 6.99e-86 | 276 |
| MS.gene09726.t1 | AT1G51440 | 41.463 | 369 | 172 | 10 | 137 | 481 | 91 | 439 | 3.66e-83 | 268 |
| MS.gene09726.t1 | AT1G06800 | 39.633 | 381 | 187 | 10 | 137 | 493 | 89 | 450 | 5.88e-79 | 257 |
| MS.gene09726.t1 | AT2G30550 | 39.227 | 362 | 186 | 7 | 137 | 479 | 105 | 451 | 9.75e-79 | 255 |
| MS.gene09726.t1 | AT1G06800 | 40.385 | 364 | 174 | 10 | 137 | 476 | 89 | 433 | 1.11e-78 | 254 |
| MS.gene09726.t1 | AT2G30550 | 38.830 | 376 | 196 | 8 | 137 | 493 | 105 | 465 | 6.99e-78 | 255 |
| MS.gene09726.t1 | AT2G31690 | 35.989 | 364 | 182 | 11 | 133 | 475 | 88 | 421 | 2.66e-65 | 220 |
| MS.gene09726.t1 | AT1G05800 | 35.833 | 360 | 185 | 9 | 133 | 475 | 80 | 410 | 1.00e-63 | 216 |
| MS.gene09726.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 19 | 397 | 1.85e-60 | 206 |
| MS.gene09726.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 36 | 414 | 2.72e-60 | 206 |
| MS.gene09726.t1 | AT1G06250 | 31.959 | 388 | 200 | 10 | 133 | 493 | 5 | 355 | 6.52e-54 | 188 |
| MS.gene09726.t1 | AT2G42690 | 32.468 | 385 | 204 | 8 | 137 | 493 | 8 | 364 | 3.55e-51 | 181 |
| MS.gene09726.t1 | AT4G18550 | 36.806 | 288 | 151 | 8 | 133 | 397 | 36 | 315 | 4.56e-47 | 167 |
| MS.gene09726.t1 | AT1G06250 | 30.797 | 276 | 140 | 7 | 234 | 493 | 1 | 241 | 5.10e-32 | 125 |
Find 139 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAATTGGATTACCTAAA+TGG | 0.127660 | 5.3:+6291069 | MS.gene09726:CDS |
| CCTCGGGTAAATCGACTAAT+TGG | 0.206931 | 5.3:-6291262 | None:intergenic |
| GAAGACGCTGTAAGCGTTTC+AGG | 0.238955 | 5.3:-6290782 | None:intergenic |
| GTTACTTTATAAGATCTTTC+CGG | 0.250527 | 5.3:-6291026 | None:intergenic |
| TAACCTAGATGAGTTGTTAT+TGG | 0.251262 | 5.3:-6290571 | None:intergenic |
| CTCTTAGCATTAGCTCTAAA+TGG | 0.258178 | 5.3:-6291890 | None:intergenic |
| CGCCTTTGTAAAGTTCAATA+AGG | 0.276950 | 5.3:-6291415 | None:intergenic |
| AAAAGATGAAGGTTGTTTAA+TGG | 0.278707 | 5.3:-6290535 | None:intergenic |
| TTTCTCCCGATGATATTTCT+TGG | 0.282496 | 5.3:-6290828 | None:intergenic |
| AGAGGCTTATTTACATTTAG+TGG | 0.289753 | 5.3:+6291844 | MS.gene09726:CDS |
| ATGATGATTCCAAAGGCTTT+TGG | 0.306541 | 5.3:-6290626 | None:intergenic |
| GCCACCTCATCAACCCATTT+AGG | 0.326253 | 5.3:-6291083 | None:intergenic |
| GTTGTATTGGTTGTTGAAAC+TGG | 0.332759 | 5.3:-6290656 | None:intergenic |
| ACTCAACTTTGGGCTTTGAT+TGG | 0.339758 | 5.3:-6291313 | None:intergenic |
| ACAGCCACGGGTGGCACGTT+TGG | 0.344135 | 5.3:-6291521 | None:intergenic |
| TAATTTCCTCGGCCACCAAT+AGG | 0.366166 | 5.3:-6291487 | None:intergenic |
| CGGCGAGAGGTTGTTAGATA+TGG | 0.368122 | 5.3:+6290915 | MS.gene09726:CDS |
| CATAAATCCACACTCAACTT+TGG | 0.371336 | 5.3:-6291324 | None:intergenic |
| CTGTAAGCGTTTCAGGTGGC+GGG | 0.378709 | 5.3:-6290775 | None:intergenic |
| TCTTCAGCTGCTTTAATCTC+TGG | 0.381476 | 5.3:-6290744 | None:intergenic |
| TGGCGCGACACTAGCCCTAT+TGG | 0.385796 | 5.3:+6291472 | MS.gene09726:CDS |
| AACTTCCACTTGTCTTGAAT+GGG | 0.386150 | 5.3:+6291223 | MS.gene09726:CDS |
| GTTGGAAGAGAACACTCCTT+TGG | 0.394244 | 5.3:+6291730 | MS.gene09726:CDS |
| CGTGTTGGTAACAGAGCCTT+TGG | 0.394565 | 5.3:+6291563 | MS.gene09726:CDS |
| TAAAAGAGAGATTGCAAGAA+TGG | 0.395214 | 5.3:+6291169 | MS.gene09726:CDS |
| ATAAATCCACACTCAACTTT+GGG | 0.396883 | 5.3:-6291323 | None:intergenic |
| TCTTGATAGCCAAAAGCCTT+TGG | 0.405866 | 5.3:+6290617 | MS.gene09726:CDS |
| GGATGACCCAGCGTTCTAGC+TGG | 0.407435 | 5.3:+6291120 | MS.gene09726:CDS |
| ATCAATTGGATTACCTAAAT+GGG | 0.408997 | 5.3:+6291070 | MS.gene09726:CDS |
| GGTTGTTAGATATGGTGATT+TGG | 0.409569 | 5.3:+6290923 | MS.gene09726:CDS |
| GAACTTCCACTTGTCTTGAA+TGG | 0.412888 | 5.3:+6291222 | MS.gene09726:CDS |
| GACTTAACCTAGCAAAACTA+TGG | 0.417487 | 5.3:+6290721 | MS.gene09726:CDS |
| CTGTATGCCACATCATCAAT+TGG | 0.418121 | 5.3:+6291056 | MS.gene09726:CDS |
| TCATCAAGAGGTGTCGTTGC+CGG | 0.419070 | 5.3:+6291007 | MS.gene09726:CDS |
| AATTTCCTCGGCCACCAATA+GGG | 0.421437 | 5.3:-6291486 | None:intergenic |
| ATGAGCTTGTACAAGACAAA+AGG | 0.423174 | 5.3:+6291344 | MS.gene09726:CDS |
| TTGGAAGAGAACACTCCTTT+GGG | 0.425192 | 5.3:+6291731 | MS.gene09726:CDS |
| GAATTAAGCATAACCGTTAC+CGG | 0.425326 | 5.3:+6291440 | MS.gene09726:CDS |
| AAGAATTCCAAGGTTGTTAG+TGG | 0.426321 | 5.3:+6291695 | MS.gene09726:CDS |
| ACATTTAGTGGATGGATACA+TGG | 0.441165 | 5.3:+6291856 | MS.gene09726:CDS |
| AAACCAATAACAACTCATCT+AGG | 0.443716 | 5.3:+6290568 | MS.gene09726:CDS |
| AATATCATGGCAGCAACGAC+TGG | 0.446528 | 5.3:+6290859 | MS.gene09726:CDS |
| GATGAGGTGGCACCAGATCT+TGG | 0.447657 | 5.3:+6291095 | MS.gene09726:CDS |
| CCCGCACGTGCTAATTTCCT+CGG | 0.448005 | 5.3:-6291498 | None:intergenic |
| TAGACAGGGATCCATGTCTA+TGG | 0.450095 | 5.3:+6292030 | MS.gene09726:CDS |
| GTTGTTTAATGGAAGAAGAA+TGG | 0.450283 | 5.3:-6290524 | None:intergenic |
| CACTTCTTCAACAACGGACT+CGG | 0.454486 | 5.3:-6291387 | None:intergenic |
| GTGAAGGTTGTGAGCAGGTA+AGG | 0.457213 | 5.3:-6290466 | None:intergenic |
| GTAAGCGTTTCAGGTGGCGG+GGG | 0.458362 | 5.3:-6290773 | None:intergenic |
| CTTGTCTAATCTTAGTAGAC+AGG | 0.459042 | 5.3:+6292015 | MS.gene09726:CDS |
| CTAGTGTCGCGCCAAGGCTA+TGG | 0.467757 | 5.3:-6291463 | None:intergenic |
| GCTGTAAGCGTTTCAGGTGG+CGG | 0.475077 | 5.3:-6290776 | None:intergenic |
| GTCTGAGTCTGAGCGTCCTC+GGG | 0.477426 | 5.3:-6291278 | None:intergenic |
| TCGCCGGAGGTTGTCATCCA+AGG | 0.480124 | 5.3:-6290898 | None:intergenic |
| GAAGGGGATGTTAGATCCCT+TGG | 0.480349 | 5.3:+6290881 | MS.gene09726:CDS |
| TGGGTCATCCAACCAAGATC+TGG | 0.480411 | 5.3:-6291107 | None:intergenic |
| TGAAGAGAAGCAAAAGATGA+AGG | 0.480894 | 5.3:-6290546 | None:intergenic |
| GCTTCAGTTGTTGACATGGC+AGG | 0.485069 | 5.3:-6290978 | None:intergenic |
| TGGAGAATACTCCGCCGTCT+TGG | 0.486287 | 5.3:-6290808 | None:intergenic |
| TATTGGTTGTTGAAACTGGT+TGG | 0.487668 | 5.3:-6290652 | None:intergenic |
| AGTCTGAGTCTGAGCGTCCT+CGG | 0.490474 | 5.3:-6291279 | None:intergenic |
| AAAAGAGAGATTGCAAGAAT+GGG | 0.490721 | 5.3:+6291170 | MS.gene09726:CDS |
| ACACACAAGACGTGATTACA+AGG | 0.492272 | 5.3:+6291630 | MS.gene09726:CDS |
| CAACGACACCTCTTGATGAT+GGG | 0.496077 | 5.3:-6291003 | None:intergenic |
| CTCGGGTAAATCGACTAATT+GGG | 0.499666 | 5.3:-6291261 | None:intergenic |
| AACAACTCCACTAACAACCT+TGG | 0.499945 | 5.3:-6291702 | None:intergenic |
| GCAACGACACCTCTTGATGA+TGG | 0.502309 | 5.3:-6291004 | None:intergenic |
| TGAGCTTGTACAAGACAAAA+GGG | 0.503559 | 5.3:+6291345 | MS.gene09726:CDS |
| CCTTTGGGATATTCACATGT+TGG | 0.504000 | 5.3:+6291746 | MS.gene09726:CDS |
| GAAATATCATCGGGAGAAAG+TGG | 0.505201 | 5.3:+6290832 | MS.gene09726:CDS |
| CTTCAACAACGGACTCGGAT+AGG | 0.508893 | 5.3:-6291382 | None:intergenic |
| TCAGCATCGGGCTTCAAGTA+AGG | 0.510052 | 5.3:-6291800 | None:intergenic |
| CAACAAGCTATGTCAGCATC+GGG | 0.515189 | 5.3:-6291812 | None:intergenic |
| GCTTATTTACATTTAGTGGA+TGG | 0.516513 | 5.3:+6291848 | MS.gene09726:CDS |
| GATGACCCAGCGTTCTAGCT+GGG | 0.518537 | 5.3:+6291121 | MS.gene09726:CDS |
| ACCCAGCGTTCTAGCTGGGT+TGG | 0.519887 | 5.3:+6291125 | MS.gene09726:CDS |
| GAAGAATGGCATCTGAAACT+AGG | 0.523536 | 5.3:-6290510 | None:intergenic |
| TTGTATACTAGTAAGGCTAA+AGG | 0.526986 | 5.3:+6291959 | MS.gene09726:CDS |
| ATCCCTTGGATGACAACCTC+CGG | 0.528647 | 5.3:+6290895 | MS.gene09726:CDS |
| ATCCAACCCAGCTAGAACGC+TGG | 0.529696 | 5.3:-6291127 | None:intergenic |
| GACGTGATTACAAGGGTGCC+AGG | 0.535150 | 5.3:+6291638 | MS.gene09726:CDS |
| CGCGACACTAGCCCTATTGG+TGG | 0.536052 | 5.3:+6291475 | MS.gene09726:CDS |
| TAGTGGAGTTGTTGATATGT+TGG | 0.537325 | 5.3:+6291712 | MS.gene09726:CDS |
| TGTAAGCGTTTCAGGTGGCG+GGG | 0.538613 | 5.3:-6290774 | None:intergenic |
| ACCGTTACCGGCCATAGCCT+TGG | 0.539736 | 5.3:+6291452 | MS.gene09726:CDS |
| ATGGCAGCAACGACTGGAAG+GGG | 0.541567 | 5.3:+6290865 | MS.gene09726:CDS |
| ACCTAAATGGGTTGATGAGG+TGG | 0.548455 | 5.3:+6291082 | MS.gene09726:CDS |
| ACTAGCCCTATTGGTGGCCG+AGG | 0.548502 | 5.3:+6291481 | MS.gene09726:CDS |
| ATTCATGAAGATGATGGACT+AGG | 0.548753 | 5.3:-6292064 | None:intergenic |
| AGGTAATCCAATTGATGATG+TGG | 0.548802 | 5.3:-6291063 | None:intergenic |
| GATATTGTGATATCTCTTCG+TGG | 0.549518 | 5.3:+6291200 | MS.gene09726:CDS |
| TCCAACCCAGCTAGAACGCT+GGG | 0.550701 | 5.3:-6291126 | None:intergenic |
| TTCAGCCCATTCAAGACAAG+TGG | 0.553127 | 5.3:-6291228 | None:intergenic |
| GGACGCTCAGACTCAGACTC+AGG | 0.556991 | 5.3:+6291283 | MS.gene09726:CDS |
| CGCCGGAGGTTGTCATCCAA+GGG | 0.557417 | 5.3:-6290897 | None:intergenic |
| ACAACAAGCTATGTCAGCAT+CGG | 0.563181 | 5.3:-6291813 | None:intergenic |
| CGGGCCAAACGTGCCACCCG+TGG | 0.563469 | 5.3:+6291517 | MS.gene09726:CDS |
| TCATGGCAGCAACGACTGGA+AGG | 0.564182 | 5.3:+6290863 | MS.gene09726:CDS |
| CGCCTTATTGAACTTTACAA+AGG | 0.564214 | 5.3:+6291413 | MS.gene09726:CDS |
| ATTCTCCAAGAAATATCATC+GGG | 0.566867 | 5.3:+6290823 | MS.gene09726:CDS |
| AGCTTGTTGTCATGATCTAG+AGG | 0.571065 | 5.3:+6291826 | MS.gene09726:CDS |
| GCCGAGGAAATTAGCACGTG+CGG | 0.571449 | 5.3:+6291497 | MS.gene09726:CDS |
| TGATGTGGCATACAGACTCT+TGG | 0.573169 | 5.3:-6291048 | None:intergenic |
| TAAGCGTTTCAGGTGGCGGG+GGG | 0.576225 | 5.3:-6290772 | None:intergenic |
| GGGTGCTTCAGTTGTTGACA+TGG | 0.576279 | 5.3:-6290982 | None:intergenic |
| CCGAGGAAATTAGCACGTGC+GGG | 0.576932 | 5.3:+6291498 | MS.gene09726:CDS |
| TCATTTGGCGGACCTCGTGT+TGG | 0.577348 | 5.3:+6291548 | MS.gene09726:CDS |
| CATATCTAACAACCTCTCGC+CGG | 0.581786 | 5.3:-6290914 | None:intergenic |
| CATGGCAGCAACGACTGGAA+GGG | 0.583943 | 5.3:+6290864 | MS.gene09726:CDS |
| AATTTGTGAAGGTTGTGAGC+AGG | 0.587076 | 5.3:-6290471 | None:intergenic |
| GCCAAGGCTATGGCCGGTAA+CGG | 0.591069 | 5.3:-6291453 | None:intergenic |
| TATTCTCCAAGAAATATCAT+CGG | 0.595846 | 5.3:+6290822 | MS.gene09726:CDS |
| AACGACACCTCTTGATGATG+GGG | 0.596360 | 5.3:-6291002 | None:intergenic |
| AGAAAGTGGAGGGAATATCA+TGG | 0.601338 | 5.3:+6290846 | MS.gene09726:CDS |
| TTGGTTGATGATGATTCCAA+AGG | 0.613659 | 5.3:-6290633 | None:intergenic |
| AGGTGGCACCAGATCTTGGT+TGG | 0.618491 | 5.3:+6291099 | MS.gene09726:CDS |
| GGATGACAACCTCCGGCGAG+AGG | 0.618831 | 5.3:+6290902 | MS.gene09726:CDS |
| CAGACTCAGACTCAGGCACA+AGG | 0.619166 | 5.3:+6291290 | MS.gene09726:CDS |
| TTAGTGCTTGAAATTTGTGA+AGG | 0.626013 | 5.3:-6290482 | None:intergenic |
| TCAAAGCCCAAAGTTGAGTG+TGG | 0.627127 | 5.3:+6291317 | MS.gene09726:CDS |
| ACAACAAGCAGCCATAGACA+TGG | 0.628855 | 5.3:-6292041 | None:intergenic |
| GCGTCGCACTTCTTCAACAA+CGG | 0.630718 | 5.3:-6291393 | None:intergenic |
| TTGTCTAATCTTAGTAGACA+GGG | 0.632883 | 5.3:+6292016 | MS.gene09726:CDS |
| ATCTAACAACCTCTCGCCGG+AGG | 0.634684 | 5.3:-6290911 | None:intergenic |
| TGAAGCACCCCATCATCAAG+AGG | 0.635362 | 5.3:+6290995 | MS.gene09726:CDS |
| TGTCGCGCCAAGGCTATGGC+CGG | 0.642253 | 5.3:-6291459 | None:intergenic |
| ACAGCGTCTTCTTTCCAAGA+CGG | 0.643880 | 5.3:+6290794 | MS.gene09726:CDS |
| TCTGTTAATCTTAATAGACA+AGG | 0.646433 | 5.3:+6291986 | MS.gene09726:CDS |
| TATCATCGGGAGAAAGTGGA+GGG | 0.648802 | 5.3:+6290836 | MS.gene09726:CDS |
| ATTACCTAAATGGGTTGATG+AGG | 0.663925 | 5.3:+6291079 | MS.gene09726:CDS |
| ATATCATCGGGAGAAAGTGG+AGG | 0.666029 | 5.3:+6290835 | MS.gene09726:CDS |
| ATAGGGCTAGTGTCGCGCCA+AGG | 0.667674 | 5.3:-6291469 | None:intergenic |
| CCAATTAGTCGATTTACCCG+AGG | 0.667868 | 5.3:+6291262 | MS.gene09726:CDS |
| GACGCTGTAAGCGTTTCAGG+TGG | 0.674999 | 5.3:-6290779 | None:intergenic |
| GCGTCTTCTTTCCAAGACGG+CGG | 0.680773 | 5.3:+6290797 | MS.gene09726:CDS |
| CCAACATGTGAATATCCCAA+AGG | 0.681313 | 5.3:-6291746 | None:intergenic |
| GAGCTTGTACAAGACAAAAG+GGG | 0.708452 | 5.3:+6291346 | MS.gene09726:CDS |
| CACACAAGACGTGATTACAA+GGG | 0.729372 | 5.3:+6291631 | MS.gene09726:CDS |
| AAGGCTCTGTTACCAACACG+AGG | 0.743509 | 5.3:-6291560 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTTGTATACTAGTA+AGG | + | chr5.3:6291952-6291971 | MS.gene09726:CDS | 20.0% |
| !! | AGAAAAAATTAAGAATTCCA+AGG | + | chr5.3:6291685-6291704 | MS.gene09726:CDS | 20.0% |
| ! | AAAAGATGAAGGTTGTTTAA+TGG | - | chr5.3:6290538-6290557 | None:intergenic | 25.0% |
| ! | AAAGGAAAAAACTTTCTTGA+AGG | + | chr5.3:6290699-6290718 | MS.gene09726:CDS | 25.0% |
| ! | ATCAATTGGATTACCTAAAT+GGG | + | chr5.3:6291070-6291089 | MS.gene09726:CDS | 25.0% |
| ! | ATCCAAAAAAGTGACAAAAA+AGG | + | chr5.3:6290681-6290700 | MS.gene09726:CDS | 25.0% |
| ! | TATTCTCCAAGAAATATCAT+CGG | + | chr5.3:6290822-6290841 | MS.gene09726:CDS | 25.0% |
| ! | TCTGTTAATCTTAATAGACA+AGG | + | chr5.3:6291986-6292005 | MS.gene09726:CDS | 25.0% |
| !! | GTTACTTTATAAGATCTTTC+CGG | - | chr5.3:6291029-6291048 | None:intergenic | 25.0% |
| !!! | TTCCTTTTTTGTCACTTTTT+TGG | - | chr5.3:6290686-6290705 | None:intergenic | 25.0% |
| !!! | TTTTTGTCACTTTTTTGGAT+AGG | - | chr5.3:6290681-6290700 | None:intergenic | 25.0% |
| AAAAGAGAGATTGCAAGAAT+GGG | + | chr5.3:6291170-6291189 | MS.gene09726:CDS | 30.0% | |
| AAACCAATAACAACTCATCT+AGG | + | chr5.3:6290568-6290587 | MS.gene09726:CDS | 30.0% | |
| AGAGGCTTATTTACATTTAG+TGG | + | chr5.3:6291844-6291863 | MS.gene09726:CDS | 30.0% | |
| ATAAATCCACACTCAACTTT+GGG | - | chr5.3:6291326-6291345 | None:intergenic | 30.0% | |
| ATTCTCCAAGAAATATCATC+GGG | + | chr5.3:6290823-6290842 | MS.gene09726:CDS | 30.0% | |
| CATCAATTGGATTACCTAAA+TGG | + | chr5.3:6291069-6291088 | MS.gene09726:CDS | 30.0% | |
| GCTTATTTACATTTAGTGGA+TGG | + | chr5.3:6291848-6291867 | MS.gene09726:CDS | 30.0% | |
| GTTGTTTAATGGAAGAAGAA+TGG | - | chr5.3:6290527-6290546 | None:intergenic | 30.0% | |
| TAAAAGAGAGATTGCAAGAA+TGG | + | chr5.3:6291169-6291188 | MS.gene09726:CDS | 30.0% | |
| TAACCTAGATGAGTTGTTAT+TGG | - | chr5.3:6290574-6290593 | None:intergenic | 30.0% | |
| TTAGTGCTTGAAATTTGTGA+AGG | - | chr5.3:6290485-6290504 | None:intergenic | 30.0% | |
| TTGTATACTAGTAAGGCTAA+AGG | + | chr5.3:6291959-6291978 | MS.gene09726:CDS | 30.0% | |
| TTGTCTAATCTTAGTAGACA+GGG | + | chr5.3:6292016-6292035 | MS.gene09726:CDS | 30.0% | |
| !!! | CTTTTTTGGATAGGTTGTAT+TGG | - | chr5.3:6290672-6290691 | None:intergenic | 30.0% |
| AACTTCCACTTGTCTTGAAT+GGG | + | chr5.3:6291223-6291242 | MS.gene09726:CDS | 35.0% | |
| AAGAATTCCAAGGTTGTTAG+TGG | + | chr5.3:6291695-6291714 | MS.gene09726:CDS | 35.0% | |
| ATGAGCTTGTACAAGACAAA+AGG | + | chr5.3:6291344-6291363 | MS.gene09726:CDS | 35.0% | |
| CATAAATCCACACTCAACTT+TGG | - | chr5.3:6291327-6291346 | None:intergenic | 35.0% | |
| CGCCTTTGTAAAGTTCAATA+AGG | - | chr5.3:6291418-6291437 | None:intergenic | 35.0% | |
| CTTGTCTAATCTTAGTAGAC+AGG | + | chr5.3:6292015-6292034 | MS.gene09726:CDS | 35.0% | |
| GAATTAAGCATAACCGTTAC+CGG | + | chr5.3:6291440-6291459 | MS.gene09726:CDS | 35.0% | |
| GACTTAACCTAGCAAAACTA+TGG | + | chr5.3:6290721-6290740 | MS.gene09726:CDS | 35.0% | |
| GATATTGTGATATCTCTTCG+TGG | + | chr5.3:6291200-6291219 | MS.gene09726:CDS | 35.0% | |
| GGTTGTTAGATATGGTGATT+TGG | + | chr5.3:6290923-6290942 | MS.gene09726:CDS | 35.0% | |
| TAGTGGAGTTGTTGATATGT+TGG | + | chr5.3:6291712-6291731 | MS.gene09726:CDS | 35.0% | |
| TCCTCGCTTAAAAATATTCC+TGG | - | chr5.3:6291659-6291678 | None:intergenic | 35.0% | |
| TGAAGAGAAGCAAAAGATGA+AGG | - | chr5.3:6290549-6290568 | None:intergenic | 35.0% | |
| TGAGCTTGTACAAGACAAAA+GGG | + | chr5.3:6291345-6291364 | MS.gene09726:CDS | 35.0% | |
| TTTCTCCCGATGATATTTCT+TGG | - | chr5.3:6290831-6290850 | None:intergenic | 35.0% | |
| ! | ACATTTAGTGGATGGATACA+TGG | + | chr5.3:6291856-6291875 | MS.gene09726:CDS | 35.0% |
| ! | ATGATGATTCCAAAGGCTTT+TGG | - | chr5.3:6290629-6290648 | None:intergenic | 35.0% |
| ! | CGCCTTATTGAACTTTACAA+AGG | + | chr5.3:6291413-6291432 | MS.gene09726:CDS | 35.0% |
| ! | TATTGGTTGTTGAAACTGGT+TGG | - | chr5.3:6290655-6290674 | None:intergenic | 35.0% |
| !! | AGGTAATCCAATTGATGATG+TGG | - | chr5.3:6291066-6291085 | None:intergenic | 35.0% |
| !! | ATTACCTAAATGGGTTGATG+AGG | + | chr5.3:6291079-6291098 | MS.gene09726:CDS | 35.0% |
| !! | CTCTTAGCATTAGCTCTAAA+TGG | - | chr5.3:6291893-6291912 | None:intergenic | 35.0% |
| !! | CTTTTCAAGATGTTCTCCAA+AGG | - | chr5.3:6291582-6291601 | None:intergenic | 35.0% |
| !! | GTTGTATTGGTTGTTGAAAC+TGG | - | chr5.3:6290659-6290678 | None:intergenic | 35.0% |
| !! | TTGGTTGATGATGATTCCAA+AGG | - | chr5.3:6290636-6290655 | None:intergenic | 35.0% |
| AACAACTCCACTAACAACCT+TGG | - | chr5.3:6291705-6291724 | None:intergenic | 40.0% | |
| AATTTGTGAAGGTTGTGAGC+AGG | - | chr5.3:6290474-6290493 | None:intergenic | 40.0% | |
| ACAACAAGCTATGTCAGCAT+CGG | - | chr5.3:6291816-6291835 | None:intergenic | 40.0% | |
| ACACACAAGACGTGATTACA+AGG | + | chr5.3:6291630-6291649 | MS.gene09726:CDS | 40.0% | |
| AGAAAGTGGAGGGAATATCA+TGG | + | chr5.3:6290846-6290865 | MS.gene09726:CDS | 40.0% | |
| CACACAAGACGTGATTACAA+GGG | + | chr5.3:6291631-6291650 | MS.gene09726:CDS | 40.0% | |
| CCAAATGAAAAAACAGCCAC+GGG | - | chr5.3:6291536-6291555 | None:intergenic | 40.0% | |
| CCAACATGTGAATATCCCAA+AGG | - | chr5.3:6291749-6291768 | None:intergenic | 40.0% | |
| CTCGGGTAAATCGACTAATT+GGG | - | chr5.3:6291264-6291283 | None:intergenic | 40.0% | |
| CTGTATGCCACATCATCAAT+TGG | + | chr5.3:6291056-6291075 | MS.gene09726:CDS | 40.0% | |
| GAAATATCATCGGGAGAAAG+TGG | + | chr5.3:6290832-6290851 | MS.gene09726:CDS | 40.0% | |
| GAACTTCCACTTGTCTTGAA+TGG | + | chr5.3:6291222-6291241 | MS.gene09726:CDS | 40.0% | |
| GAGCTTGTACAAGACAAAAG+GGG | + | chr5.3:6291346-6291365 | MS.gene09726:CDS | 40.0% | |
| GCCAAATGAAAAAACAGCCA+CGG | - | chr5.3:6291537-6291556 | None:intergenic | 40.0% | |
| TTGGAAGAGAACACTCCTTT+GGG | + | chr5.3:6291731-6291750 | MS.gene09726:CDS | 40.0% | |
| ! | ACTCAACTTTGGGCTTTGAT+TGG | - | chr5.3:6291316-6291335 | None:intergenic | 40.0% |
| ! | GAAGAATGGCATCTGAAACT+AGG | - | chr5.3:6290513-6290532 | None:intergenic | 40.0% |
| ! | TCTTCAGCTGCTTTAATCTC+TGG | - | chr5.3:6290747-6290766 | None:intergenic | 40.0% |
| ! | TCTTGATAGCCAAAAGCCTT+TGG | + | chr5.3:6290617-6290636 | MS.gene09726:CDS | 40.0% |
| !! | AGCTTGTTGTCATGATCTAG+AGG | + | chr5.3:6291826-6291845 | MS.gene09726:CDS | 40.0% |
| !! | CCTTTGGGATATTCACATGT+TGG | + | chr5.3:6291746-6291765 | MS.gene09726:CDS | 40.0% |
| !! | GCCAGGAATATTTTTAAGCG+AGG | + | chr5.3:6291655-6291674 | MS.gene09726:CDS | 40.0% |
| !!! | GTGGCTGTTTTTTCATTTGG+CGG | + | chr5.3:6291536-6291555 | MS.gene09726:CDS | 40.0% |
| AATATCATGGCAGCAACGAC+TGG | + | chr5.3:6290859-6290878 | MS.gene09726:CDS | 45.0% | |
| AATGAAAAAACAGCCACGGG+TGG | - | chr5.3:6291533-6291552 | None:intergenic | 45.0% | |
| AATTTCCTCGGCCACCAATA+GGG | - | chr5.3:6291489-6291508 | None:intergenic | 45.0% | |
| ACAACAAGCAGCCATAGACA+TGG | - | chr5.3:6292044-6292063 | None:intergenic | 45.0% | |
| ACAGCGTCTTCTTTCCAAGA+CGG | + | chr5.3:6290794-6290813 | MS.gene09726:CDS | 45.0% | |
| ATATCATCGGGAGAAAGTGG+AGG | + | chr5.3:6290835-6290854 | MS.gene09726:CDS | 45.0% | |
| CAACAAGCTATGTCAGCATC+GGG | - | chr5.3:6291815-6291834 | None:intergenic | 45.0% | |
| CATATCTAACAACCTCTCGC+CGG | - | chr5.3:6290917-6290936 | None:intergenic | 45.0% | |
| CCTCGGGTAAATCGACTAAT+TGG | - | chr5.3:6291265-6291284 | None:intergenic | 45.0% | |
| GTTGGAAGAGAACACTCCTT+TGG | + | chr5.3:6291730-6291749 | MS.gene09726:CDS | 45.0% | |
| TAATTTCCTCGGCCACCAAT+AGG | - | chr5.3:6291490-6291509 | None:intergenic | 45.0% | |
| TAGACAGGGATCCATGTCTA+TGG | + | chr5.3:6292030-6292049 | MS.gene09726:CDS | 45.0% | |
| TATCATCGGGAGAAAGTGGA+GGG | + | chr5.3:6290836-6290855 | MS.gene09726:CDS | 45.0% | |
| TCAAAGCCCAAAGTTGAGTG+TGG | + | chr5.3:6291317-6291336 | MS.gene09726:CDS | 45.0% | |
| TGATGTGGCATACAGACTCT+TGG | - | chr5.3:6291051-6291070 | None:intergenic | 45.0% | |
| TTCAGCCCATTCAAGACAAG+TGG | - | chr5.3:6291231-6291250 | None:intergenic | 45.0% | |
| ! | CAACGACACCTCTTGATGAT+GGG | - | chr5.3:6291006-6291025 | None:intergenic | 45.0% |
| ! | CACTTCTTCAACAACGGACT+CGG | - | chr5.3:6291390-6291409 | None:intergenic | 45.0% |
| ! | CCAATTAGTCGATTTACCCG+AGG | + | chr5.3:6291262-6291281 | MS.gene09726:CDS | 45.0% |
| !! | AACGACACCTCTTGATGATG+GGG | - | chr5.3:6291005-6291024 | None:intergenic | 45.0% |
| !! | ACCTAAATGGGTTGATGAGG+TGG | + | chr5.3:6291082-6291101 | MS.gene09726:CDS | 45.0% |
| !! | TCTCTGGCCATAGTTTTGCT+AGG | - | chr5.3:6290731-6290750 | None:intergenic | 45.0% |
| !!! | CCCGTGGCTGTTTTTTCATT+TGG | + | chr5.3:6291533-6291552 | MS.gene09726:CDS | 45.0% |
| AAGGCTCTGTTACCAACACG+AGG | - | chr5.3:6291563-6291582 | None:intergenic | 50.0% | |
| ATCCCTTGGATGACAACCTC+CGG | + | chr5.3:6290895-6290914 | MS.gene09726:CDS | 50.0% | |
| CGGCGAGAGGTTGTTAGATA+TGG | + | chr5.3:6290915-6290934 | MS.gene09726:CDS | 50.0% | |
| GAAGACGCTGTAAGCGTTTC+AGG | - | chr5.3:6290785-6290804 | None:intergenic | 50.0% | |
| GAAGGGGATGTTAGATCCCT+TGG | + | chr5.3:6290881-6290900 | MS.gene09726:CDS | 50.0% | |
| GCAACGACACCTCTTGATGA+TGG | - | chr5.3:6291007-6291026 | None:intergenic | 50.0% | |
| GCCACCTCATCAACCCATTT+AGG | - | chr5.3:6291086-6291105 | None:intergenic | 50.0% | |
| GCGTCGCACTTCTTCAACAA+CGG | - | chr5.3:6291396-6291415 | None:intergenic | 50.0% | |
| GCTTCAGTTGTTGACATGGC+AGG | - | chr5.3:6290981-6291000 | None:intergenic | 50.0% | |
| GGGTGCTTCAGTTGTTGACA+TGG | - | chr5.3:6290985-6291004 | None:intergenic | 50.0% | |
| GTGAAGGTTGTGAGCAGGTA+AGG | - | chr5.3:6290469-6290488 | None:intergenic | 50.0% | |
| TGGGTCATCCAACCAAGATC+TGG | - | chr5.3:6291110-6291129 | None:intergenic | 50.0% | |
| ! | CTTCAACAACGGACTCGGAT+AGG | - | chr5.3:6291385-6291404 | None:intergenic | 50.0% |
| ! | TCAGCATCGGGCTTCAAGTA+AGG | - | chr5.3:6291803-6291822 | None:intergenic | 50.0% |
| ! | TGAAGCACCCCATCATCAAG+AGG | + | chr5.3:6290995-6291014 | MS.gene09726:CDS | 50.0% |
| !! | CGTGTTGGTAACAGAGCCTT+TGG | + | chr5.3:6291563-6291582 | MS.gene09726:CDS | 50.0% |
| !! | TCATCAAGAGGTGTCGTTGC+CGG | + | chr5.3:6291007-6291026 | MS.gene09726:CDS | 50.0% |
| AGGTGGCACCAGATCTTGGT+TGG | + | chr5.3:6291099-6291118 | MS.gene09726:CDS | 55.0% | |
| AGTCTGAGTCTGAGCGTCCT+CGG | - | chr5.3:6291282-6291301 | None:intergenic | 55.0% | |
| ATCCAACCCAGCTAGAACGC+TGG | - | chr5.3:6291130-6291149 | None:intergenic | 55.0% | |
| ATCTAACAACCTCTCGCCGG+AGG | - | chr5.3:6290914-6290933 | None:intergenic | 55.0% | |
| ATGGCAGCAACGACTGGAAG+GGG | + | chr5.3:6290865-6290884 | MS.gene09726:CDS | 55.0% | |
| CAGACTCAGACTCAGGCACA+AGG | + | chr5.3:6291290-6291309 | MS.gene09726:CDS | 55.0% | |
| CATGGCAGCAACGACTGGAA+GGG | + | chr5.3:6290864-6290883 | MS.gene09726:CDS | 55.0% | |
| CCCGCACGTGCTAATTTCCT+CGG | - | chr5.3:6291501-6291520 | None:intergenic | 55.0% | |
| CCGAGGAAATTAGCACGTGC+GGG | + | chr5.3:6291498-6291517 | MS.gene09726:CDS | 55.0% | |
| GACGCTGTAAGCGTTTCAGG+TGG | - | chr5.3:6290782-6290801 | None:intergenic | 55.0% | |
| GATGAGGTGGCACCAGATCT+TGG | + | chr5.3:6291095-6291114 | MS.gene09726:CDS | 55.0% | |
| GCCGAGGAAATTAGCACGTG+CGG | + | chr5.3:6291497-6291516 | MS.gene09726:CDS | 55.0% | |
| GCGTCTTCTTTCCAAGACGG+CGG | + | chr5.3:6290797-6290816 | MS.gene09726:CDS | 55.0% | |
| TCATGGCAGCAACGACTGGA+AGG | + | chr5.3:6290863-6290882 | MS.gene09726:CDS | 55.0% | |
| TCCAACCCAGCTAGAACGCT+GGG | - | chr5.3:6291129-6291148 | None:intergenic | 55.0% | |
| TGGAGAATACTCCGCCGTCT+TGG | - | chr5.3:6290811-6290830 | None:intergenic | 55.0% | |
| ! | CTGTAAGCGTTTCAGGTGGC+GGG | - | chr5.3:6290778-6290797 | None:intergenic | 55.0% |
| ! | GATGACCCAGCGTTCTAGCT+GGG | + | chr5.3:6291121-6291140 | MS.gene09726:CDS | 55.0% |
| ! | GCTGTAAGCGTTTCAGGTGG+CGG | - | chr5.3:6290779-6290798 | None:intergenic | 55.0% |
| ! | TCATTTGGCGGACCTCGTGT+TGG | + | chr5.3:6291548-6291567 | MS.gene09726:CDS | 55.0% |
| ! | TGTAAGCGTTTCAGGTGGCG+GGG | - | chr5.3:6290777-6290796 | None:intergenic | 55.0% |
| !! | GACGTGATTACAAGGGTGCC+AGG | + | chr5.3:6291638-6291657 | MS.gene09726:CDS | 55.0% |
| ACCGTTACCGGCCATAGCCT+TGG | + | chr5.3:6291452-6291471 | MS.gene09726:CDS | 60.0% | |
| CGCCGGAGGTTGTCATCCAA+GGG | - | chr5.3:6290900-6290919 | None:intergenic | 60.0% | |
| CGCGACACTAGCCCTATTGG+TGG | + | chr5.3:6291475-6291494 | MS.gene09726:CDS | 60.0% | |
| CTAGTGTCGCGCCAAGGCTA+TGG | - | chr5.3:6291466-6291485 | None:intergenic | 60.0% | |
| GCCAAGGCTATGGCCGGTAA+CGG | - | chr5.3:6291456-6291475 | None:intergenic | 60.0% | |
| GGACGCTCAGACTCAGACTC+AGG | + | chr5.3:6291283-6291302 | MS.gene09726:CDS | 60.0% | |
| GGATGACCCAGCGTTCTAGC+TGG | + | chr5.3:6291120-6291139 | MS.gene09726:CDS | 60.0% | |
| GTCTGAGTCTGAGCGTCCTC+GGG | - | chr5.3:6291281-6291300 | None:intergenic | 60.0% | |
| TCGCCGGAGGTTGTCATCCA+AGG | - | chr5.3:6290901-6290920 | None:intergenic | 60.0% | |
| TGGCGCGACACTAGCCCTAT+TGG | + | chr5.3:6291472-6291491 | MS.gene09726:CDS | 60.0% | |
| ! | ACCCAGCGTTCTAGCTGGGT+TGG | + | chr5.3:6291125-6291144 | MS.gene09726:CDS | 60.0% |
| ! | ACTAGCCCTATTGGTGGCCG+AGG | + | chr5.3:6291481-6291500 | MS.gene09726:CDS | 60.0% |
| ! | ATAGGGCTAGTGTCGCGCCA+AGG | - | chr5.3:6291472-6291491 | None:intergenic | 60.0% |
| ! | GTAAGCGTTTCAGGTGGCGG+GGG | - | chr5.3:6290776-6290795 | None:intergenic | 60.0% |
| !! | TAAGCGTTTCAGGTGGCGGG+GGG | - | chr5.3:6290775-6290794 | None:intergenic | 60.0% |
| ACAGCCACGGGTGGCACGTT+TGG | - | chr5.3:6291524-6291543 | None:intergenic | 65.0% | |
| GGATGACAACCTCCGGCGAG+AGG | + | chr5.3:6290902-6290921 | MS.gene09726:CDS | 65.0% | |
| TGTCGCGCCAAGGCTATGGC+CGG | - | chr5.3:6291462-6291481 | None:intergenic | 65.0% | |
| CGGGCCAAACGTGCCACCCG+TGG | + | chr5.3:6291517-6291536 | MS.gene09726:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6290444 | 6292084 | 6290444 | ID=MS.gene09726 |
| chr5.3 | mRNA | 6290444 | 6292084 | 6290444 | ID=MS.gene09726.t1;Parent=MS.gene09726 |
| chr5.3 | exon | 6290444 | 6292084 | 6290444 | ID=MS.gene09726.t1.exon1;Parent=MS.gene09726.t1 |
| chr5.3 | CDS | 6290444 | 6292084 | 6290444 | ID=cds.MS.gene09726.t1;Parent=MS.gene09726.t1 |
| Gene Sequence |
| Protein sequence |
