Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09807.t1 | XP_003625582.1 | 100 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 7.10E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09807.t1 | P49201 | 96.5 | 142 | 5 | 0 | 1 | 142 | 1 | 142 | 2.1e-73 | 276.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09807.t1 | B7FHD2 | 100.0 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 5.1e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050290 | MS.gene09807 | 0.820277 | 7.47E-53 | -1.69E-46 |
| MS.gene050916 | MS.gene09807 | 0.835269 | 1.85E-56 | -1.69E-46 |
| MS.gene054937 | MS.gene09807 | 0.822715 | 2.04E-53 | -1.69E-46 |
| MS.gene06001 | MS.gene09807 | 0.809461 | 1.87E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09807.t1 | MTR_7g100720 | 100.000 | 142 | 0 | 0 | 1 | 142 | 1 | 142 | 5.97e-101 | 285 |
| MS.gene09807.t1 | MTR_1g066860 | 93.662 | 142 | 9 | 0 | 1 | 142 | 1 | 142 | 1.98e-94 | 269 |
| MS.gene09807.t1 | MTR_1g066860 | 94.118 | 136 | 8 | 0 | 7 | 142 | 1 | 136 | 1.66e-90 | 259 |
| MS.gene09807.t1 | MTR_7g061155 | 99.153 | 118 | 1 | 0 | 25 | 142 | 138 | 255 | 6.91e-82 | 241 |
| MS.gene09807.t1 | MTR_7g063460 | 52.857 | 70 | 30 | 2 | 40 | 108 | 13 | 80 | 1.73e-15 | 67.4 |
| MS.gene09807.t1 | MTR_8g447270 | 64.151 | 53 | 17 | 1 | 54 | 106 | 17 | 67 | 2.67e-14 | 65.1 |
| MS.gene09807.t1 | MTR_4g038400 | 67.347 | 49 | 13 | 1 | 56 | 104 | 103 | 148 | 2.41e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09807.t1 | AT5G02960 | 96.479 | 142 | 5 | 0 | 1 | 142 | 1 | 142 | 3.92e-98 | 278 |
| MS.gene09807.t1 | AT3G09680 | 92.958 | 142 | 10 | 0 | 1 | 142 | 1 | 142 | 2.03e-93 | 266 |
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAAGGTCGTGAAGGTTTC+TGG | 0.178129 | 7.1:+7784395 | MS.gene09807:CDS |
| GAAGTCTTGATCGCTGGATT+TGG | 0.312989 | 7.1:+7784332 | MS.gene09807:CDS |
| TAATTCAAGCAACCATCATT+TGG | 0.323107 | 7.1:-7784159 | None:intergenic |
| CATGCTGTTGGTGATATTCC+TGG | 0.406074 | 7.1:+7784365 | MS.gene09807:CDS |
| AATTCAAGCAACCATCATTT+GGG | 0.428558 | 7.1:-7784158 | None:intergenic |
| CACTTACGAATGGCAGAGTT+GGG | 0.434931 | 7.1:-7784087 | None:intergenic |
| CTGCCATTCGTAAGTGTGCT+AGG | 0.435504 | 7.1:+7784094 | MS.gene09807:CDS |
| TTGGTGATATTCCTGGAGTC+AGG | 0.441270 | 7.1:+7784372 | MS.gene09807:CDS |
| AGCATGGGATGAACCAGAAA+AGG | 0.446685 | 7.1:-7783916 | None:intergenic |
| TTCTGGTTCATCCCATGCTA+AGG | 0.454550 | 7.1:+7783920 | MS.gene09807:CDS |
| AATTTAAGAAATCTCATCTT+GGG | 0.454713 | 7.1:+7783877 | MS.gene09807:CDS |
| AATCTCATCTTGGGAATGAA+TGG | 0.457554 | 7.1:+7783886 | MS.gene09807:CDS |
| CAATTTAAGAAATCTCATCT+TGG | 0.461581 | 7.1:+7783876 | MS.gene09807:CDS |
| CAGCCCACCTTTGCTTGATA+CGG | 0.462913 | 7.1:-7783848 | None:intergenic |
| TAGGATGAAGTCTTGATCGC+TGG | 0.469665 | 7.1:+7784326 | MS.gene09807:intron |
| GCTGCCTTTGTCCCAAATGA+TGG | 0.476668 | 7.1:+7784147 | MS.gene09807:CDS |
| TGCAGGAAGACAAGAGGAAT+GGG | 0.492442 | 7.1:+7783801 | MS.gene09807:intron |
| CAAGAACAATTCCCTTAGCA+TGG | 0.494037 | 7.1:-7783932 | None:intergenic |
| ACACTTACGAATGGCAGAGT+TGG | 0.515105 | 7.1:-7784088 | None:intergenic |
| ATCGCTGGATTTGGACGTAA+AGG | 0.518707 | 7.1:+7784341 | MS.gene09807:CDS |
| GCATGGGATGAACCAGAAAA+GGG | 0.518943 | 7.1:-7783915 | None:intergenic |
| AGGAGAAGAAGGAGAAGCCT+AGG | 0.519158 | 7.1:+7784441 | MS.gene09807:CDS |
| TATTCCTGGAGTCAGGTTCA+AGG | 0.524415 | 7.1:+7784379 | MS.gene09807:CDS |
| CTGCAGGAAGACAAGAGGAA+TGG | 0.532735 | 7.1:+7783800 | MS.gene09807:intron |
| AAGAACAATTCCCTTAGCAT+GGG | 0.533822 | 7.1:-7783931 | None:intergenic |
| TCTGGTTCATCCCATGCTAA+GGG | 0.536691 | 7.1:+7783921 | MS.gene09807:CDS |
| TGCCATTCGTAAGTGTGCTA+GGG | 0.546102 | 7.1:+7784095 | MS.gene09807:CDS |
| GCAACCATCATTTGGGACAA+AGG | 0.566362 | 7.1:-7784151 | None:intergenic |
| TGGGAGCTGCTCGCAAGCTG+AGG | 0.577320 | 7.1:+7783820 | MS.gene09807:CDS |
| ACGACCTTGAACCTGACTCC+AGG | 0.578153 | 7.1:-7784383 | None:intergenic |
| GACTCCGTATCAAGCAAAGG+TGG | 0.586634 | 7.1:+7783844 | MS.gene09807:CDS |
| TCTTCTCCTTGAAAAGAGCA+AGG | 0.589001 | 7.1:-7784427 | None:intergenic |
| TTTGTCTGCAGGAAGACAAG+AGG | 0.606196 | 7.1:+7783795 | MS.gene09807:intron |
| GGACGTAAAGGTCATGCTGT+TGG | 0.616141 | 7.1:+7784353 | MS.gene09807:CDS |
| AACCCTAGCACACTTACGAA+TGG | 0.626506 | 7.1:-7784097 | None:intergenic |
| TGGTTGCTTGAATTACATTG+AGG | 0.642212 | 7.1:+7784167 | MS.gene09807:CDS |
| AGTCAGGTTCAAGGTCGTGA+AGG | 0.647492 | 7.1:+7784388 | MS.gene09807:CDS |
| ACTCCGTATCAAGCAAAGGT+GGG | 0.652737 | 7.1:+7783845 | MS.gene09807:CDS |
| GGAGACTCCGTATCAAGCAA+AGG | 0.654928 | 7.1:+7783841 | MS.gene09807:CDS |
| ATCCCACAACTTCAAGACCT+AGG | 0.665200 | 7.1:-7784458 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAAAAATTTAGTTAATTTT+AGG | + | chr7.1:7783705-7783724 | MS.gene09807:intron | 10.0% |
| !!! | ATTTGTATATTTCTAGTATT+AGG | + | chr7.1:7783549-7783568 | MS.gene09807:intron | 15.0% |
| !! | AAGATATATTTAGTAGTAAG+AGG | - | chr7.1:7783980-7783999 | None:intergenic | 20.0% |
| !! | AATTTAAGAAATCTCATCTT+GGG | + | chr7.1:7783877-7783896 | MS.gene09807:CDS | 20.0% |
| !! | TGTAGTTTACATAGTATTAA+AGG | - | chr7.1:7784266-7784285 | None:intergenic | 20.0% |
| !!! | AATTTAGTTAATTTTAGGCA+TGG | + | chr7.1:7783710-7783729 | MS.gene09807:intron | 20.0% |
| ! | AATTAACATAACAGACTCAA+AGG | - | chr7.1:7784240-7784259 | None:intergenic | 25.0% |
| ! | AGGGTTCAATTAATCAAAAA+TGG | + | chr7.1:7784114-7784133 | MS.gene09807:CDS | 25.0% |
| ! | CAATTTAAGAAATCTCATCT+TGG | + | chr7.1:7783876-7783895 | MS.gene09807:CDS | 25.0% |
| ! | GGGTTCAATTAATCAAAAAT+GGG | + | chr7.1:7784115-7784134 | MS.gene09807:CDS | 25.0% |
| ! | TATGATGTTAAGGATTTGTA+TGG | + | chr7.1:7784031-7784050 | MS.gene09807:intron | 25.0% |
| ! | TTATTACGCATATGATGTTA+AGG | + | chr7.1:7784021-7784040 | MS.gene09807:intron | 25.0% |
| ! | TTCAAATACGACAATTAGAA+TGG | - | chr7.1:7783349-7783368 | None:intergenic | 25.0% |
| ! | TTTGAATTTGACTCTGAATT+TGG | + | chr7.1:7783363-7783382 | MS.gene09807:intron | 25.0% |
| !!! | GAATTTTTTGCTACTTAGTA+TGG | + | chr7.1:7783485-7783504 | MS.gene09807:intron | 25.0% |
| AATTCAAGCAACCATCATTT+GGG | - | chr7.1:7784161-7784180 | None:intergenic | 30.0% | |
| ATCATCAACAACCATATTAC+TGG | - | chr7.1:7784300-7784319 | None:intergenic | 30.0% | |
| ATGTATATCCTCCAGTAATA+TGG | + | chr7.1:7784286-7784305 | MS.gene09807:intron | 30.0% | |
| GGAACACAAAAACATGAAAT+AGG | + | chr7.1:7783429-7783448 | MS.gene09807:intron | 30.0% | |
| TAATTCAAGCAACCATCATT+TGG | - | chr7.1:7784162-7784181 | None:intergenic | 30.0% | |
| ! | AGTAATATGGTTGTTGATGA+TGG | + | chr7.1:7784299-7784318 | MS.gene09807:intron | 30.0% |
| ! | ATTCGCTTCATTTGAAATCA+TGG | + | chr7.1:7783585-7783604 | MS.gene09807:intron | 30.0% |
| ! | TTTGTTTATCTTTTGTCTGC+AGG | + | chr7.1:7783784-7783803 | MS.gene09807:intron | 30.0% |
| ! | TTTTATGTGTAGTGGTATTG+AGG | + | chr7.1:7784056-7784075 | MS.gene09807:intron | 30.0% |
| AAGAACAATTCCCTTAGCAT+GGG | - | chr7.1:7783934-7783953 | None:intergenic | 35.0% | |
| AATCTCATCTTGGGAATGAA+TGG | + | chr7.1:7783886-7783905 | MS.gene09807:CDS | 35.0% | |
| ATCAACAACCATATTACTGG+AGG | - | chr7.1:7784297-7784316 | None:intergenic | 35.0% | |
| TGGTTGCTTGAATTACATTG+AGG | + | chr7.1:7784167-7784186 | MS.gene09807:CDS | 35.0% | |
| ! | CTGTTTGCTTTCTGAGAATT+GGG | + | chr7.1:7783408-7783427 | MS.gene09807:intron | 35.0% |
| ! | GTATGGTCTTTTATGTGTAG+TGG | + | chr7.1:7784048-7784067 | MS.gene09807:intron | 35.0% |
| ! | TCTGTTTGCTTTCTGAGAAT+TGG | + | chr7.1:7783407-7783426 | MS.gene09807:intron | 35.0% |
| CAAGAACAATTCCCTTAGCA+TGG | - | chr7.1:7783935-7783954 | None:intergenic | 40.0% | |
| TCTTCTCCTTGAAAAGAGCA+AGG | - | chr7.1:7784430-7784449 | None:intergenic | 40.0% | |
| ! | GAATGGAAGAAACCCTTTTC+TGG | + | chr7.1:7783903-7783922 | MS.gene09807:CDS | 40.0% |
| !! | GGTTGTTGATGATGGTTTGT+AGG | + | chr7.1:7784307-7784326 | MS.gene09807:intron | 40.0% |
| !! | TGCTCTTTTCAAGGAGAAGA+AGG | + | chr7.1:7784430-7784449 | MS.gene09807:CDS | 40.0% |
| AACCCTAGCACACTTACGAA+TGG | - | chr7.1:7784100-7784119 | None:intergenic | 45.0% | |
| ACACTTACGAATGGCAGAGT+TGG | - | chr7.1:7784091-7784110 | None:intergenic | 45.0% | |
| ACTCCGTATCAAGCAAAGGT+GGG | + | chr7.1:7783845-7783864 | MS.gene09807:CDS | 45.0% | |
| AGCATGGGATGAACCAGAAA+AGG | - | chr7.1:7783919-7783938 | None:intergenic | 45.0% | |
| ATCGCTGGATTTGGACGTAA+AGG | + | chr7.1:7784341-7784360 | MS.gene09807:CDS | 45.0% | |
| CACTTACGAATGGCAGAGTT+GGG | - | chr7.1:7784090-7784109 | None:intergenic | 45.0% | |
| GAAGTCTTGATCGCTGGATT+TGG | + | chr7.1:7784332-7784351 | MS.gene09807:CDS | 45.0% | |
| GCATGGGATGAACCAGAAAA+GGG | - | chr7.1:7783918-7783937 | None:intergenic | 45.0% | |
| TAGGATGAAGTCTTGATCGC+TGG | + | chr7.1:7784326-7784345 | MS.gene09807:intron | 45.0% | |
| TATTCCTGGAGTCAGGTTCA+AGG | + | chr7.1:7784379-7784398 | MS.gene09807:CDS | 45.0% | |
| TGCAGGAAGACAAGAGGAAT+GGG | + | chr7.1:7783801-7783820 | MS.gene09807:intron | 45.0% | |
| TGCCATTCGTAAGTGTGCTA+GGG | + | chr7.1:7784095-7784114 | MS.gene09807:CDS | 45.0% | |
| TTCAAGGTCGTGAAGGTTTC+TGG | + | chr7.1:7784395-7784414 | MS.gene09807:CDS | 45.0% | |
| TTTGTCTGCAGGAAGACAAG+AGG | + | chr7.1:7783795-7783814 | MS.gene09807:intron | 45.0% | |
| ! | CTCTCTCCTTGCTCTTTTCA+AGG | + | chr7.1:7784421-7784440 | MS.gene09807:CDS | 45.0% |
| ! | GCAACCATCATTTGGGACAA+AGG | - | chr7.1:7784154-7784173 | None:intergenic | 45.0% |
| ! | TCTGGTTCATCCCATGCTAA+GGG | + | chr7.1:7783921-7783940 | MS.gene09807:CDS | 45.0% |
| ! | TTGGTGATATTCCTGGAGTC+AGG | + | chr7.1:7784372-7784391 | MS.gene09807:CDS | 45.0% |
| !! | CATGCTGTTGGTGATATTCC+TGG | + | chr7.1:7784365-7784384 | MS.gene09807:CDS | 45.0% |
| !! | TTCTGGTTCATCCCATGCTA+AGG | + | chr7.1:7783920-7783939 | MS.gene09807:CDS | 45.0% |
| AGGAGAAGAAGGAGAAGCCT+AGG | + | chr7.1:7784441-7784460 | MS.gene09807:CDS | 50.0% | |
| AGTCAGGTTCAAGGTCGTGA+AGG | + | chr7.1:7784388-7784407 | MS.gene09807:CDS | 50.0% | |
| CAGCCCACCTTTGCTTGATA+CGG | - | chr7.1:7783851-7783870 | None:intergenic | 50.0% | |
| CTGCAGGAAGACAAGAGGAA+TGG | + | chr7.1:7783800-7783819 | MS.gene09807:intron | 50.0% | |
| CTGCCATTCGTAAGTGTGCT+AGG | + | chr7.1:7784094-7784113 | MS.gene09807:CDS | 50.0% | |
| GACTCCGTATCAAGCAAAGG+TGG | + | chr7.1:7783844-7783863 | MS.gene09807:CDS | 50.0% | |
| GCTGCCTTTGTCCCAAATGA+TGG | + | chr7.1:7784147-7784166 | MS.gene09807:CDS | 50.0% | |
| GGACGTAAAGGTCATGCTGT+TGG | + | chr7.1:7784353-7784372 | MS.gene09807:CDS | 50.0% | |
| GGAGACTCCGTATCAAGCAA+AGG | + | chr7.1:7783841-7783860 | MS.gene09807:CDS | 50.0% | |
| ACGACCTTGAACCTGACTCC+AGG | - | chr7.1:7784386-7784405 | None:intergenic | 55.0% | |
| TGGGAGCTGCTCGCAAGCTG+AGG | + | chr7.1:7783820-7783839 | MS.gene09807:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7783273 | 7784469 | 7783273 | ID=MS.gene09807 |
| chr7.1 | mRNA | 7783273 | 7784469 | 7783273 | ID=MS.gene09807.t1;Parent=MS.gene09807 |
| chr7.1 | exon | 7783273 | 7783277 | 7783273 | ID=MS.gene09807.t1.exon1;Parent=MS.gene09807.t1 |
| chr7.1 | CDS | 7783273 | 7783277 | 7783273 | ID=cds.MS.gene09807.t1;Parent=MS.gene09807.t1 |
| chr7.1 | exon | 7783806 | 7783961 | 7783806 | ID=MS.gene09807.t1.exon2;Parent=MS.gene09807.t1 |
| chr7.1 | CDS | 7783806 | 7783961 | 7783806 | ID=cds.MS.gene09807.t1;Parent=MS.gene09807.t1 |
| chr7.1 | exon | 7784068 | 7784194 | 7784068 | ID=MS.gene09807.t1.exon3;Parent=MS.gene09807.t1 |
| chr7.1 | CDS | 7784068 | 7784194 | 7784068 | ID=cds.MS.gene09807.t1;Parent=MS.gene09807.t1 |
| chr7.1 | exon | 7784329 | 7784469 | 7784329 | ID=MS.gene09807.t1.exon4;Parent=MS.gene09807.t1 |
| chr7.1 | CDS | 7784329 | 7784469 | 7784329 | ID=cds.MS.gene09807.t1;Parent=MS.gene09807.t1 |
| Gene Sequence |
| Protein sequence |
