Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09922.t1 | XP_003611369.1 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 8.80E-297 | 1028.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09922.t1 | O23522 | 55.4 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 5.3e-157 | 555.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09922.t1 | G7JXH0 | 96.7 | 548 | 16 | 2 | 1 | 546 | 1 | 548 | 6.3e-297 | 1028.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050238 | MS.gene09922 | 0.81528 | 1.00E-51 | -1.69E-46 |
| MS.gene051823 | MS.gene09922 | 0.81096 | 8.89E-51 | -1.69E-46 |
| MS.gene052016 | MS.gene09922 | 0.829151 | 6.04E-55 | -1.69E-46 |
| MS.gene054178 | MS.gene09922 | 0.803263 | 3.79E-49 | -1.69E-46 |
| MS.gene060522 | MS.gene09922 | 0.807508 | 4.88E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09922.t1 | MTR_5g013230 | 97.628 | 548 | 11 | 2 | 1 | 546 | 1 | 548 | 0.0 | 1110 |
| MS.gene09922.t1 | MTR_4g099510 | 65.766 | 555 | 137 | 9 | 6 | 544 | 4 | 521 | 0.0 | 715 |
| MS.gene09922.t1 | MTR_7g081620 | 47.165 | 388 | 177 | 8 | 129 | 492 | 65 | 448 | 1.57e-120 | 362 |
| MS.gene09922.t1 | MTR_7g081645 | 45.553 | 371 | 167 | 9 | 133 | 492 | 75 | 421 | 8.94e-110 | 334 |
| MS.gene09922.t1 | MTR_4g077070 | 38.281 | 384 | 201 | 7 | 128 | 493 | 95 | 460 | 3.97e-84 | 271 |
| MS.gene09922.t1 | MTR_7g096120 | 39.737 | 380 | 194 | 11 | 133 | 493 | 74 | 437 | 1.12e-81 | 264 |
| MS.gene09922.t1 | MTR_7g096140 | 39.211 | 380 | 196 | 11 | 133 | 493 | 74 | 437 | 5.42e-81 | 261 |
| MS.gene09922.t1 | MTR_2g015690 | 35.156 | 384 | 192 | 9 | 133 | 493 | 20 | 369 | 1.28e-63 | 214 |
| MS.gene09922.t1 | MTR_7g006400 | 32.683 | 410 | 215 | 13 | 109 | 500 | 80 | 446 | 4.17e-57 | 199 |
| MS.gene09922.t1 | MTR_4g087810 | 33.075 | 387 | 195 | 11 | 133 | 495 | 13 | 359 | 6.13e-52 | 182 |
| MS.gene09922.t1 | MTR_4g087830 | 33.333 | 387 | 194 | 12 | 133 | 495 | 4 | 350 | 2.82e-51 | 181 |
| MS.gene09922.t1 | MTR_4g080100 | 29.353 | 402 | 214 | 9 | 129 | 493 | 24 | 392 | 1.01e-48 | 175 |
| MS.gene09922.t1 | MTR_1995s0010 | 40.928 | 237 | 114 | 7 | 251 | 475 | 1 | 223 | 5.20e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09922.t1 | AT4G16820 | 55.367 | 531 | 197 | 10 | 25 | 546 | 18 | 517 | 0.0 | 562 |
| MS.gene09922.t1 | AT2G44810 | 50.136 | 369 | 170 | 7 | 133 | 494 | 86 | 447 | 5.25e-123 | 369 |
| MS.gene09922.t1 | AT2G44810 | 50.413 | 363 | 166 | 7 | 139 | 494 | 2 | 357 | 1.95e-120 | 359 |
| MS.gene09922.t1 | AT1G30370 | 38.015 | 413 | 219 | 10 | 124 | 517 | 105 | 499 | 6.99e-86 | 276 |
| MS.gene09922.t1 | AT1G51440 | 41.463 | 369 | 172 | 10 | 137 | 481 | 91 | 439 | 3.66e-83 | 268 |
| MS.gene09922.t1 | AT1G06800 | 39.633 | 381 | 187 | 10 | 137 | 493 | 89 | 450 | 5.88e-79 | 257 |
| MS.gene09922.t1 | AT2G30550 | 39.227 | 362 | 186 | 7 | 137 | 479 | 105 | 451 | 9.75e-79 | 255 |
| MS.gene09922.t1 | AT1G06800 | 40.385 | 364 | 174 | 10 | 137 | 476 | 89 | 433 | 1.11e-78 | 254 |
| MS.gene09922.t1 | AT2G30550 | 38.830 | 376 | 196 | 8 | 137 | 493 | 105 | 465 | 6.99e-78 | 255 |
| MS.gene09922.t1 | AT2G31690 | 35.989 | 364 | 182 | 11 | 133 | 475 | 88 | 421 | 2.66e-65 | 220 |
| MS.gene09922.t1 | AT1G05800 | 35.833 | 360 | 185 | 9 | 133 | 475 | 80 | 410 | 1.00e-63 | 216 |
| MS.gene09922.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 19 | 397 | 1.85e-60 | 206 |
| MS.gene09922.t1 | AT4G18550 | 33.012 | 415 | 210 | 11 | 133 | 515 | 36 | 414 | 2.72e-60 | 206 |
| MS.gene09922.t1 | AT1G06250 | 31.959 | 388 | 200 | 10 | 133 | 493 | 5 | 355 | 6.52e-54 | 188 |
| MS.gene09922.t1 | AT2G42690 | 32.468 | 385 | 204 | 8 | 137 | 493 | 8 | 364 | 3.55e-51 | 181 |
| MS.gene09922.t1 | AT4G18550 | 36.806 | 288 | 151 | 8 | 133 | 397 | 36 | 315 | 4.56e-47 | 167 |
| MS.gene09922.t1 | AT1G06250 | 30.797 | 276 | 140 | 7 | 234 | 493 | 1 | 241 | 5.10e-32 | 125 |
Find 139 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATCAATTGGATTACCTAAA+TGG | 0.127660 | 5.3:+6155813 | MS.gene09922:CDS |
| CCTCGGGTAAATCGACTAAT+TGG | 0.206931 | 5.3:-6156006 | None:intergenic |
| GAAGACGCTGTAAGCGTTTC+AGG | 0.238955 | 5.3:-6155526 | None:intergenic |
| GTTACTTTATAAGATCTTTC+CGG | 0.250527 | 5.3:-6155770 | None:intergenic |
| TAACCTAGATGAGTTGTTAT+TGG | 0.251262 | 5.3:-6155315 | None:intergenic |
| CTCTTAGCATTAGCTCTAAA+TGG | 0.258178 | 5.3:-6156634 | None:intergenic |
| CGCCTTTGTAAAGTTCAATA+AGG | 0.276950 | 5.3:-6156159 | None:intergenic |
| AAAAGATGAAGGTTGTTTAA+TGG | 0.278707 | 5.3:-6155279 | None:intergenic |
| TTTCTCCCGATGATATTTCT+TGG | 0.282496 | 5.3:-6155572 | None:intergenic |
| AGAGGCTTATTTACATTTAG+TGG | 0.289753 | 5.3:+6156588 | MS.gene09922:CDS |
| ATGATGATTCCAAAGGCTTT+TGG | 0.306541 | 5.3:-6155370 | None:intergenic |
| GCCACCTCATCAACCCATTT+AGG | 0.326253 | 5.3:-6155827 | None:intergenic |
| GTTGTATTGGTTGTTGAAAC+TGG | 0.332759 | 5.3:-6155400 | None:intergenic |
| ACTCAACTTTGGGCTTTGAT+TGG | 0.339758 | 5.3:-6156057 | None:intergenic |
| ACAGCCACGGGTGGCACGTT+TGG | 0.344135 | 5.3:-6156265 | None:intergenic |
| TAATTTCCTCGGCCACCAAT+AGG | 0.366166 | 5.3:-6156231 | None:intergenic |
| CGGCGAGAGGTTGTTAGATA+TGG | 0.368122 | 5.3:+6155659 | MS.gene09922:CDS |
| CATAAATCCACACTCAACTT+TGG | 0.371336 | 5.3:-6156068 | None:intergenic |
| CTGTAAGCGTTTCAGGTGGC+GGG | 0.378709 | 5.3:-6155519 | None:intergenic |
| TCTTCAGCTGCTTTAATCTC+TGG | 0.381476 | 5.3:-6155488 | None:intergenic |
| TGGCGCGACACTAGCCCTAT+TGG | 0.385796 | 5.3:+6156216 | MS.gene09922:CDS |
| AACTTCCACTTGTCTTGAAT+GGG | 0.386150 | 5.3:+6155967 | MS.gene09922:CDS |
| GTTGGAAGAGAACACTCCTT+TGG | 0.394244 | 5.3:+6156474 | MS.gene09922:CDS |
| CGTGTTGGTAACAGAGCCTT+TGG | 0.394565 | 5.3:+6156307 | MS.gene09922:CDS |
| TAAAAGAGAGATTGCAAGAA+TGG | 0.395214 | 5.3:+6155913 | MS.gene09922:CDS |
| ATAAATCCACACTCAACTTT+GGG | 0.396883 | 5.3:-6156067 | None:intergenic |
| TCTTGATAGCCAAAAGCCTT+TGG | 0.405866 | 5.3:+6155361 | MS.gene09922:CDS |
| GGATGACCCAGCGTTCTAGC+TGG | 0.407435 | 5.3:+6155864 | MS.gene09922:CDS |
| ATCAATTGGATTACCTAAAT+GGG | 0.408997 | 5.3:+6155814 | MS.gene09922:CDS |
| GGTTGTTAGATATGGTGATT+TGG | 0.409569 | 5.3:+6155667 | MS.gene09922:CDS |
| GAACTTCCACTTGTCTTGAA+TGG | 0.412888 | 5.3:+6155966 | MS.gene09922:CDS |
| GACTTAACCTAGCAAAACTA+TGG | 0.417487 | 5.3:+6155465 | MS.gene09922:CDS |
| CTGTATGCCACATCATCAAT+TGG | 0.418121 | 5.3:+6155800 | MS.gene09922:CDS |
| TCATCAAGAGGTGTCGTTGC+CGG | 0.419070 | 5.3:+6155751 | MS.gene09922:CDS |
| AATTTCCTCGGCCACCAATA+GGG | 0.421437 | 5.3:-6156230 | None:intergenic |
| ATGAGCTTGTACAAGACAAA+AGG | 0.423174 | 5.3:+6156088 | MS.gene09922:CDS |
| TTGGAAGAGAACACTCCTTT+GGG | 0.425192 | 5.3:+6156475 | MS.gene09922:CDS |
| GAATTAAGCATAACCGTTAC+CGG | 0.425326 | 5.3:+6156184 | MS.gene09922:CDS |
| AAGAATTCCAAGGTTGTTAG+TGG | 0.426321 | 5.3:+6156439 | MS.gene09922:CDS |
| ACATTTAGTGGATGGATACA+TGG | 0.441165 | 5.3:+6156600 | MS.gene09922:CDS |
| AAACCAATAACAACTCATCT+AGG | 0.443716 | 5.3:+6155312 | MS.gene09922:CDS |
| AATATCATGGCAGCAACGAC+TGG | 0.446528 | 5.3:+6155603 | MS.gene09922:CDS |
| GATGAGGTGGCACCAGATCT+TGG | 0.447657 | 5.3:+6155839 | MS.gene09922:CDS |
| CCCGCACGTGCTAATTTCCT+CGG | 0.448005 | 5.3:-6156242 | None:intergenic |
| TAGACAGGGATCCATGTCTA+TGG | 0.450095 | 5.3:+6156774 | MS.gene09922:CDS |
| GTTGTTTAATGGAAGAAGAA+TGG | 0.450283 | 5.3:-6155268 | None:intergenic |
| CACTTCTTCAACAACGGACT+CGG | 0.454486 | 5.3:-6156131 | None:intergenic |
| GTGAAGGTTGTGAGCAGGTA+AGG | 0.457213 | 5.3:-6155210 | None:intergenic |
| GTAAGCGTTTCAGGTGGCGG+GGG | 0.458362 | 5.3:-6155517 | None:intergenic |
| CTTGTCTAATCTTAGTAGAC+AGG | 0.459042 | 5.3:+6156759 | MS.gene09922:CDS |
| CTAGTGTCGCGCCAAGGCTA+TGG | 0.467757 | 5.3:-6156207 | None:intergenic |
| GCTGTAAGCGTTTCAGGTGG+CGG | 0.475077 | 5.3:-6155520 | None:intergenic |
| GTCTGAGTCTGAGCGTCCTC+GGG | 0.477426 | 5.3:-6156022 | None:intergenic |
| TCGCCGGAGGTTGTCATCCA+AGG | 0.480124 | 5.3:-6155642 | None:intergenic |
| GAAGGGGATGTTAGATCCCT+TGG | 0.480349 | 5.3:+6155625 | MS.gene09922:CDS |
| TGGGTCATCCAACCAAGATC+TGG | 0.480411 | 5.3:-6155851 | None:intergenic |
| TGAAGAGAAGCAAAAGATGA+AGG | 0.480894 | 5.3:-6155290 | None:intergenic |
| GCTTCAGTTGTTGACATGGC+AGG | 0.485069 | 5.3:-6155722 | None:intergenic |
| TGGAGAATACTCCGCCGTCT+TGG | 0.486287 | 5.3:-6155552 | None:intergenic |
| TATTGGTTGTTGAAACTGGT+TGG | 0.487668 | 5.3:-6155396 | None:intergenic |
| AGTCTGAGTCTGAGCGTCCT+CGG | 0.490474 | 5.3:-6156023 | None:intergenic |
| AAAAGAGAGATTGCAAGAAT+GGG | 0.490721 | 5.3:+6155914 | MS.gene09922:CDS |
| ACACACAAGACGTGATTACA+AGG | 0.492272 | 5.3:+6156374 | MS.gene09922:CDS |
| CAACGACACCTCTTGATGAT+GGG | 0.496077 | 5.3:-6155747 | None:intergenic |
| CTCGGGTAAATCGACTAATT+GGG | 0.499666 | 5.3:-6156005 | None:intergenic |
| AACAACTCCACTAACAACCT+TGG | 0.499945 | 5.3:-6156446 | None:intergenic |
| GCAACGACACCTCTTGATGA+TGG | 0.502309 | 5.3:-6155748 | None:intergenic |
| TGAGCTTGTACAAGACAAAA+GGG | 0.503559 | 5.3:+6156089 | MS.gene09922:CDS |
| CCTTTGGGATATTCACATGT+TGG | 0.504000 | 5.3:+6156490 | MS.gene09922:CDS |
| GAAATATCATCGGGAGAAAG+TGG | 0.505201 | 5.3:+6155576 | MS.gene09922:CDS |
| CTTCAACAACGGACTCGGAT+AGG | 0.508893 | 5.3:-6156126 | None:intergenic |
| TCAGCATCGGGCTTCAAGTA+AGG | 0.510052 | 5.3:-6156544 | None:intergenic |
| CAACAAGCTATGTCAGCATC+GGG | 0.515189 | 5.3:-6156556 | None:intergenic |
| GCTTATTTACATTTAGTGGA+TGG | 0.516513 | 5.3:+6156592 | MS.gene09922:CDS |
| GATGACCCAGCGTTCTAGCT+GGG | 0.518537 | 5.3:+6155865 | MS.gene09922:CDS |
| ACCCAGCGTTCTAGCTGGGT+TGG | 0.519887 | 5.3:+6155869 | MS.gene09922:CDS |
| GAAGAATGGCATCTGAAACT+AGG | 0.523536 | 5.3:-6155254 | None:intergenic |
| TTGTATACTAGTAAGGCTAA+AGG | 0.526986 | 5.3:+6156703 | MS.gene09922:CDS |
| ATCCCTTGGATGACAACCTC+CGG | 0.528647 | 5.3:+6155639 | MS.gene09922:CDS |
| ATCCAACCCAGCTAGAACGC+TGG | 0.529696 | 5.3:-6155871 | None:intergenic |
| GACGTGATTACAAGGGTGCC+AGG | 0.535150 | 5.3:+6156382 | MS.gene09922:CDS |
| CGCGACACTAGCCCTATTGG+TGG | 0.536052 | 5.3:+6156219 | MS.gene09922:CDS |
| TAGTGGAGTTGTTGATATGT+TGG | 0.537325 | 5.3:+6156456 | MS.gene09922:CDS |
| TGTAAGCGTTTCAGGTGGCG+GGG | 0.538613 | 5.3:-6155518 | None:intergenic |
| ACCGTTACCGGCCATAGCCT+TGG | 0.539736 | 5.3:+6156196 | MS.gene09922:CDS |
| ATGGCAGCAACGACTGGAAG+GGG | 0.541567 | 5.3:+6155609 | MS.gene09922:CDS |
| ACCTAAATGGGTTGATGAGG+TGG | 0.548455 | 5.3:+6155826 | MS.gene09922:CDS |
| ACTAGCCCTATTGGTGGCCG+AGG | 0.548502 | 5.3:+6156225 | MS.gene09922:CDS |
| ATTCATGAAGATGATGGACT+AGG | 0.548753 | 5.3:-6156808 | None:intergenic |
| AGGTAATCCAATTGATGATG+TGG | 0.548802 | 5.3:-6155807 | None:intergenic |
| GATATTGTGATATCTCTTCG+TGG | 0.549518 | 5.3:+6155944 | MS.gene09922:CDS |
| TCCAACCCAGCTAGAACGCT+GGG | 0.550701 | 5.3:-6155870 | None:intergenic |
| TTCAGCCCATTCAAGACAAG+TGG | 0.553127 | 5.3:-6155972 | None:intergenic |
| GGACGCTCAGACTCAGACTC+AGG | 0.556991 | 5.3:+6156027 | MS.gene09922:CDS |
| CGCCGGAGGTTGTCATCCAA+GGG | 0.557417 | 5.3:-6155641 | None:intergenic |
| ACAACAAGCTATGTCAGCAT+CGG | 0.563181 | 5.3:-6156557 | None:intergenic |
| CGGGCCAAACGTGCCACCCG+TGG | 0.563469 | 5.3:+6156261 | MS.gene09922:CDS |
| TCATGGCAGCAACGACTGGA+AGG | 0.564182 | 5.3:+6155607 | MS.gene09922:CDS |
| CGCCTTATTGAACTTTACAA+AGG | 0.564214 | 5.3:+6156157 | MS.gene09922:CDS |
| ATTCTCCAAGAAATATCATC+GGG | 0.566867 | 5.3:+6155567 | MS.gene09922:CDS |
| AGCTTGTTGTCATGATCTAG+AGG | 0.571065 | 5.3:+6156570 | MS.gene09922:CDS |
| GCCGAGGAAATTAGCACGTG+CGG | 0.571449 | 5.3:+6156241 | MS.gene09922:CDS |
| TGATGTGGCATACAGACTCT+TGG | 0.573169 | 5.3:-6155792 | None:intergenic |
| TAAGCGTTTCAGGTGGCGGG+GGG | 0.576225 | 5.3:-6155516 | None:intergenic |
| GGGTGCTTCAGTTGTTGACA+TGG | 0.576279 | 5.3:-6155726 | None:intergenic |
| CCGAGGAAATTAGCACGTGC+GGG | 0.576932 | 5.3:+6156242 | MS.gene09922:CDS |
| TCATTTGGCGGACCTCGTGT+TGG | 0.577348 | 5.3:+6156292 | MS.gene09922:CDS |
| CATATCTAACAACCTCTCGC+CGG | 0.581786 | 5.3:-6155658 | None:intergenic |
| CATGGCAGCAACGACTGGAA+GGG | 0.583943 | 5.3:+6155608 | MS.gene09922:CDS |
| AATTTGTGAAGGTTGTGAGC+AGG | 0.587076 | 5.3:-6155215 | None:intergenic |
| GCCAAGGCTATGGCCGGTAA+CGG | 0.591069 | 5.3:-6156197 | None:intergenic |
| TATTCTCCAAGAAATATCAT+CGG | 0.595846 | 5.3:+6155566 | MS.gene09922:CDS |
| AACGACACCTCTTGATGATG+GGG | 0.596360 | 5.3:-6155746 | None:intergenic |
| AGAAAGTGGAGGGAATATCA+TGG | 0.601338 | 5.3:+6155590 | MS.gene09922:CDS |
| TTGGTTGATGATGATTCCAA+AGG | 0.613659 | 5.3:-6155377 | None:intergenic |
| AGGTGGCACCAGATCTTGGT+TGG | 0.618491 | 5.3:+6155843 | MS.gene09922:CDS |
| GGATGACAACCTCCGGCGAG+AGG | 0.618831 | 5.3:+6155646 | MS.gene09922:CDS |
| CAGACTCAGACTCAGGCACA+AGG | 0.619166 | 5.3:+6156034 | MS.gene09922:CDS |
| TTAGTGCTTGAAATTTGTGA+AGG | 0.626013 | 5.3:-6155226 | None:intergenic |
| TCAAAGCCCAAAGTTGAGTG+TGG | 0.627127 | 5.3:+6156061 | MS.gene09922:CDS |
| ACAACAAGCAGCCATAGACA+TGG | 0.628855 | 5.3:-6156785 | None:intergenic |
| GCGTCGCACTTCTTCAACAA+CGG | 0.630718 | 5.3:-6156137 | None:intergenic |
| TTGTCTAATCTTAGTAGACA+GGG | 0.632883 | 5.3:+6156760 | MS.gene09922:CDS |
| ATCTAACAACCTCTCGCCGG+AGG | 0.634684 | 5.3:-6155655 | None:intergenic |
| TGAAGCACCCCATCATCAAG+AGG | 0.635362 | 5.3:+6155739 | MS.gene09922:CDS |
| TGTCGCGCCAAGGCTATGGC+CGG | 0.642253 | 5.3:-6156203 | None:intergenic |
| ACAGCGTCTTCTTTCCAAGA+CGG | 0.643880 | 5.3:+6155538 | MS.gene09922:CDS |
| TCTGTTAATCTTAATAGACA+AGG | 0.646433 | 5.3:+6156730 | MS.gene09922:CDS |
| TATCATCGGGAGAAAGTGGA+GGG | 0.648802 | 5.3:+6155580 | MS.gene09922:CDS |
| ATTACCTAAATGGGTTGATG+AGG | 0.663925 | 5.3:+6155823 | MS.gene09922:CDS |
| ATATCATCGGGAGAAAGTGG+AGG | 0.666029 | 5.3:+6155579 | MS.gene09922:CDS |
| ATAGGGCTAGTGTCGCGCCA+AGG | 0.667674 | 5.3:-6156213 | None:intergenic |
| CCAATTAGTCGATTTACCCG+AGG | 0.667868 | 5.3:+6156006 | MS.gene09922:CDS |
| GACGCTGTAAGCGTTTCAGG+TGG | 0.674999 | 5.3:-6155523 | None:intergenic |
| GCGTCTTCTTTCCAAGACGG+CGG | 0.680773 | 5.3:+6155541 | MS.gene09922:CDS |
| CCAACATGTGAATATCCCAA+AGG | 0.681313 | 5.3:-6156490 | None:intergenic |
| GAGCTTGTACAAGACAAAAG+GGG | 0.708452 | 5.3:+6156090 | MS.gene09922:CDS |
| CACACAAGACGTGATTACAA+GGG | 0.729372 | 5.3:+6156375 | MS.gene09922:CDS |
| AAGGCTCTGTTACCAACACG+AGG | 0.743509 | 5.3:-6156304 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTTGTATACTAGTA+AGG | + | chr5.3:6156696-6156715 | MS.gene09922:CDS | 20.0% |
| !! | AGAAAAAATTAAGAATTCCA+AGG | + | chr5.3:6156429-6156448 | MS.gene09922:CDS | 20.0% |
| ! | AAAAGATGAAGGTTGTTTAA+TGG | - | chr5.3:6155282-6155301 | None:intergenic | 25.0% |
| ! | AAAGGAAAAAACTTTCTTGA+AGG | + | chr5.3:6155443-6155462 | MS.gene09922:CDS | 25.0% |
| ! | ATCAATTGGATTACCTAAAT+GGG | + | chr5.3:6155814-6155833 | MS.gene09922:CDS | 25.0% |
| ! | ATCCAAAAAAGTGACAAAAA+AGG | + | chr5.3:6155425-6155444 | MS.gene09922:CDS | 25.0% |
| ! | TATTCTCCAAGAAATATCAT+CGG | + | chr5.3:6155566-6155585 | MS.gene09922:CDS | 25.0% |
| ! | TCTGTTAATCTTAATAGACA+AGG | + | chr5.3:6156730-6156749 | MS.gene09922:CDS | 25.0% |
| !! | GTTACTTTATAAGATCTTTC+CGG | - | chr5.3:6155773-6155792 | None:intergenic | 25.0% |
| !!! | TTCCTTTTTTGTCACTTTTT+TGG | - | chr5.3:6155430-6155449 | None:intergenic | 25.0% |
| !!! | TTTTTGTCACTTTTTTGGAT+AGG | - | chr5.3:6155425-6155444 | None:intergenic | 25.0% |
| AAAAGAGAGATTGCAAGAAT+GGG | + | chr5.3:6155914-6155933 | MS.gene09922:CDS | 30.0% | |
| AAACCAATAACAACTCATCT+AGG | + | chr5.3:6155312-6155331 | MS.gene09922:CDS | 30.0% | |
| AGAGGCTTATTTACATTTAG+TGG | + | chr5.3:6156588-6156607 | MS.gene09922:CDS | 30.0% | |
| ATAAATCCACACTCAACTTT+GGG | - | chr5.3:6156070-6156089 | None:intergenic | 30.0% | |
| ATTCTCCAAGAAATATCATC+GGG | + | chr5.3:6155567-6155586 | MS.gene09922:CDS | 30.0% | |
| CATCAATTGGATTACCTAAA+TGG | + | chr5.3:6155813-6155832 | MS.gene09922:CDS | 30.0% | |
| GCTTATTTACATTTAGTGGA+TGG | + | chr5.3:6156592-6156611 | MS.gene09922:CDS | 30.0% | |
| GTTGTTTAATGGAAGAAGAA+TGG | - | chr5.3:6155271-6155290 | None:intergenic | 30.0% | |
| TAAAAGAGAGATTGCAAGAA+TGG | + | chr5.3:6155913-6155932 | MS.gene09922:CDS | 30.0% | |
| TAACCTAGATGAGTTGTTAT+TGG | - | chr5.3:6155318-6155337 | None:intergenic | 30.0% | |
| TTAGTGCTTGAAATTTGTGA+AGG | - | chr5.3:6155229-6155248 | None:intergenic | 30.0% | |
| TTGTATACTAGTAAGGCTAA+AGG | + | chr5.3:6156703-6156722 | MS.gene09922:CDS | 30.0% | |
| TTGTCTAATCTTAGTAGACA+GGG | + | chr5.3:6156760-6156779 | MS.gene09922:CDS | 30.0% | |
| !!! | CTTTTTTGGATAGGTTGTAT+TGG | - | chr5.3:6155416-6155435 | None:intergenic | 30.0% |
| AACTTCCACTTGTCTTGAAT+GGG | + | chr5.3:6155967-6155986 | MS.gene09922:CDS | 35.0% | |
| AAGAATTCCAAGGTTGTTAG+TGG | + | chr5.3:6156439-6156458 | MS.gene09922:CDS | 35.0% | |
| ATGAGCTTGTACAAGACAAA+AGG | + | chr5.3:6156088-6156107 | MS.gene09922:CDS | 35.0% | |
| CATAAATCCACACTCAACTT+TGG | - | chr5.3:6156071-6156090 | None:intergenic | 35.0% | |
| CGCCTTTGTAAAGTTCAATA+AGG | - | chr5.3:6156162-6156181 | None:intergenic | 35.0% | |
| CTTGTCTAATCTTAGTAGAC+AGG | + | chr5.3:6156759-6156778 | MS.gene09922:CDS | 35.0% | |
| GAATTAAGCATAACCGTTAC+CGG | + | chr5.3:6156184-6156203 | MS.gene09922:CDS | 35.0% | |
| GACTTAACCTAGCAAAACTA+TGG | + | chr5.3:6155465-6155484 | MS.gene09922:CDS | 35.0% | |
| GATATTGTGATATCTCTTCG+TGG | + | chr5.3:6155944-6155963 | MS.gene09922:CDS | 35.0% | |
| GGTTGTTAGATATGGTGATT+TGG | + | chr5.3:6155667-6155686 | MS.gene09922:CDS | 35.0% | |
| TAGTGGAGTTGTTGATATGT+TGG | + | chr5.3:6156456-6156475 | MS.gene09922:CDS | 35.0% | |
| TCCTCGCTTAAAAATATTCC+TGG | - | chr5.3:6156403-6156422 | None:intergenic | 35.0% | |
| TGAAGAGAAGCAAAAGATGA+AGG | - | chr5.3:6155293-6155312 | None:intergenic | 35.0% | |
| TGAGCTTGTACAAGACAAAA+GGG | + | chr5.3:6156089-6156108 | MS.gene09922:CDS | 35.0% | |
| TTTCTCCCGATGATATTTCT+TGG | - | chr5.3:6155575-6155594 | None:intergenic | 35.0% | |
| ! | ACATTTAGTGGATGGATACA+TGG | + | chr5.3:6156600-6156619 | MS.gene09922:CDS | 35.0% |
| ! | ATGATGATTCCAAAGGCTTT+TGG | - | chr5.3:6155373-6155392 | None:intergenic | 35.0% |
| ! | CGCCTTATTGAACTTTACAA+AGG | + | chr5.3:6156157-6156176 | MS.gene09922:CDS | 35.0% |
| ! | TATTGGTTGTTGAAACTGGT+TGG | - | chr5.3:6155399-6155418 | None:intergenic | 35.0% |
| !! | AGGTAATCCAATTGATGATG+TGG | - | chr5.3:6155810-6155829 | None:intergenic | 35.0% |
| !! | ATTACCTAAATGGGTTGATG+AGG | + | chr5.3:6155823-6155842 | MS.gene09922:CDS | 35.0% |
| !! | CTCTTAGCATTAGCTCTAAA+TGG | - | chr5.3:6156637-6156656 | None:intergenic | 35.0% |
| !! | CTTTTCAAGATGTTCTCCAA+AGG | - | chr5.3:6156326-6156345 | None:intergenic | 35.0% |
| !! | GTTGTATTGGTTGTTGAAAC+TGG | - | chr5.3:6155403-6155422 | None:intergenic | 35.0% |
| !! | TTGGTTGATGATGATTCCAA+AGG | - | chr5.3:6155380-6155399 | None:intergenic | 35.0% |
| AACAACTCCACTAACAACCT+TGG | - | chr5.3:6156449-6156468 | None:intergenic | 40.0% | |
| AATTTGTGAAGGTTGTGAGC+AGG | - | chr5.3:6155218-6155237 | None:intergenic | 40.0% | |
| ACAACAAGCTATGTCAGCAT+CGG | - | chr5.3:6156560-6156579 | None:intergenic | 40.0% | |
| ACACACAAGACGTGATTACA+AGG | + | chr5.3:6156374-6156393 | MS.gene09922:CDS | 40.0% | |
| AGAAAGTGGAGGGAATATCA+TGG | + | chr5.3:6155590-6155609 | MS.gene09922:CDS | 40.0% | |
| CACACAAGACGTGATTACAA+GGG | + | chr5.3:6156375-6156394 | MS.gene09922:CDS | 40.0% | |
| CCAAATGAAAAAACAGCCAC+GGG | - | chr5.3:6156280-6156299 | None:intergenic | 40.0% | |
| CCAACATGTGAATATCCCAA+AGG | - | chr5.3:6156493-6156512 | None:intergenic | 40.0% | |
| CTCGGGTAAATCGACTAATT+GGG | - | chr5.3:6156008-6156027 | None:intergenic | 40.0% | |
| CTGTATGCCACATCATCAAT+TGG | + | chr5.3:6155800-6155819 | MS.gene09922:CDS | 40.0% | |
| GAAATATCATCGGGAGAAAG+TGG | + | chr5.3:6155576-6155595 | MS.gene09922:CDS | 40.0% | |
| GAACTTCCACTTGTCTTGAA+TGG | + | chr5.3:6155966-6155985 | MS.gene09922:CDS | 40.0% | |
| GAGCTTGTACAAGACAAAAG+GGG | + | chr5.3:6156090-6156109 | MS.gene09922:CDS | 40.0% | |
| GCCAAATGAAAAAACAGCCA+CGG | - | chr5.3:6156281-6156300 | None:intergenic | 40.0% | |
| TTGGAAGAGAACACTCCTTT+GGG | + | chr5.3:6156475-6156494 | MS.gene09922:CDS | 40.0% | |
| ! | ACTCAACTTTGGGCTTTGAT+TGG | - | chr5.3:6156060-6156079 | None:intergenic | 40.0% |
| ! | GAAGAATGGCATCTGAAACT+AGG | - | chr5.3:6155257-6155276 | None:intergenic | 40.0% |
| ! | TCTTCAGCTGCTTTAATCTC+TGG | - | chr5.3:6155491-6155510 | None:intergenic | 40.0% |
| ! | TCTTGATAGCCAAAAGCCTT+TGG | + | chr5.3:6155361-6155380 | MS.gene09922:CDS | 40.0% |
| !! | AGCTTGTTGTCATGATCTAG+AGG | + | chr5.3:6156570-6156589 | MS.gene09922:CDS | 40.0% |
| !! | CCTTTGGGATATTCACATGT+TGG | + | chr5.3:6156490-6156509 | MS.gene09922:CDS | 40.0% |
| !! | GCCAGGAATATTTTTAAGCG+AGG | + | chr5.3:6156399-6156418 | MS.gene09922:CDS | 40.0% |
| !!! | GTGGCTGTTTTTTCATTTGG+CGG | + | chr5.3:6156280-6156299 | MS.gene09922:CDS | 40.0% |
| AATATCATGGCAGCAACGAC+TGG | + | chr5.3:6155603-6155622 | MS.gene09922:CDS | 45.0% | |
| AATGAAAAAACAGCCACGGG+TGG | - | chr5.3:6156277-6156296 | None:intergenic | 45.0% | |
| AATTTCCTCGGCCACCAATA+GGG | - | chr5.3:6156233-6156252 | None:intergenic | 45.0% | |
| ACAACAAGCAGCCATAGACA+TGG | - | chr5.3:6156788-6156807 | None:intergenic | 45.0% | |
| ACAGCGTCTTCTTTCCAAGA+CGG | + | chr5.3:6155538-6155557 | MS.gene09922:CDS | 45.0% | |
| ATATCATCGGGAGAAAGTGG+AGG | + | chr5.3:6155579-6155598 | MS.gene09922:CDS | 45.0% | |
| CAACAAGCTATGTCAGCATC+GGG | - | chr5.3:6156559-6156578 | None:intergenic | 45.0% | |
| CATATCTAACAACCTCTCGC+CGG | - | chr5.3:6155661-6155680 | None:intergenic | 45.0% | |
| CCTCGGGTAAATCGACTAAT+TGG | - | chr5.3:6156009-6156028 | None:intergenic | 45.0% | |
| GTTGGAAGAGAACACTCCTT+TGG | + | chr5.3:6156474-6156493 | MS.gene09922:CDS | 45.0% | |
| TAATTTCCTCGGCCACCAAT+AGG | - | chr5.3:6156234-6156253 | None:intergenic | 45.0% | |
| TAGACAGGGATCCATGTCTA+TGG | + | chr5.3:6156774-6156793 | MS.gene09922:CDS | 45.0% | |
| TATCATCGGGAGAAAGTGGA+GGG | + | chr5.3:6155580-6155599 | MS.gene09922:CDS | 45.0% | |
| TCAAAGCCCAAAGTTGAGTG+TGG | + | chr5.3:6156061-6156080 | MS.gene09922:CDS | 45.0% | |
| TGATGTGGCATACAGACTCT+TGG | - | chr5.3:6155795-6155814 | None:intergenic | 45.0% | |
| TTCAGCCCATTCAAGACAAG+TGG | - | chr5.3:6155975-6155994 | None:intergenic | 45.0% | |
| ! | CAACGACACCTCTTGATGAT+GGG | - | chr5.3:6155750-6155769 | None:intergenic | 45.0% |
| ! | CACTTCTTCAACAACGGACT+CGG | - | chr5.3:6156134-6156153 | None:intergenic | 45.0% |
| ! | CCAATTAGTCGATTTACCCG+AGG | + | chr5.3:6156006-6156025 | MS.gene09922:CDS | 45.0% |
| !! | AACGACACCTCTTGATGATG+GGG | - | chr5.3:6155749-6155768 | None:intergenic | 45.0% |
| !! | ACCTAAATGGGTTGATGAGG+TGG | + | chr5.3:6155826-6155845 | MS.gene09922:CDS | 45.0% |
| !! | TCTCTGGCCATAGTTTTGCT+AGG | - | chr5.3:6155475-6155494 | None:intergenic | 45.0% |
| !!! | CCCGTGGCTGTTTTTTCATT+TGG | + | chr5.3:6156277-6156296 | MS.gene09922:CDS | 45.0% |
| AAGGCTCTGTTACCAACACG+AGG | - | chr5.3:6156307-6156326 | None:intergenic | 50.0% | |
| ATCCCTTGGATGACAACCTC+CGG | + | chr5.3:6155639-6155658 | MS.gene09922:CDS | 50.0% | |
| CGGCGAGAGGTTGTTAGATA+TGG | + | chr5.3:6155659-6155678 | MS.gene09922:CDS | 50.0% | |
| GAAGACGCTGTAAGCGTTTC+AGG | - | chr5.3:6155529-6155548 | None:intergenic | 50.0% | |
| GAAGGGGATGTTAGATCCCT+TGG | + | chr5.3:6155625-6155644 | MS.gene09922:CDS | 50.0% | |
| GCAACGACACCTCTTGATGA+TGG | - | chr5.3:6155751-6155770 | None:intergenic | 50.0% | |
| GCCACCTCATCAACCCATTT+AGG | - | chr5.3:6155830-6155849 | None:intergenic | 50.0% | |
| GCGTCGCACTTCTTCAACAA+CGG | - | chr5.3:6156140-6156159 | None:intergenic | 50.0% | |
| GCTTCAGTTGTTGACATGGC+AGG | - | chr5.3:6155725-6155744 | None:intergenic | 50.0% | |
| GGGTGCTTCAGTTGTTGACA+TGG | - | chr5.3:6155729-6155748 | None:intergenic | 50.0% | |
| GTGAAGGTTGTGAGCAGGTA+AGG | - | chr5.3:6155213-6155232 | None:intergenic | 50.0% | |
| TGGGTCATCCAACCAAGATC+TGG | - | chr5.3:6155854-6155873 | None:intergenic | 50.0% | |
| ! | CTTCAACAACGGACTCGGAT+AGG | - | chr5.3:6156129-6156148 | None:intergenic | 50.0% |
| ! | TCAGCATCGGGCTTCAAGTA+AGG | - | chr5.3:6156547-6156566 | None:intergenic | 50.0% |
| ! | TGAAGCACCCCATCATCAAG+AGG | + | chr5.3:6155739-6155758 | MS.gene09922:CDS | 50.0% |
| !! | CGTGTTGGTAACAGAGCCTT+TGG | + | chr5.3:6156307-6156326 | MS.gene09922:CDS | 50.0% |
| !! | TCATCAAGAGGTGTCGTTGC+CGG | + | chr5.3:6155751-6155770 | MS.gene09922:CDS | 50.0% |
| AGGTGGCACCAGATCTTGGT+TGG | + | chr5.3:6155843-6155862 | MS.gene09922:CDS | 55.0% | |
| AGTCTGAGTCTGAGCGTCCT+CGG | - | chr5.3:6156026-6156045 | None:intergenic | 55.0% | |
| ATCCAACCCAGCTAGAACGC+TGG | - | chr5.3:6155874-6155893 | None:intergenic | 55.0% | |
| ATCTAACAACCTCTCGCCGG+AGG | - | chr5.3:6155658-6155677 | None:intergenic | 55.0% | |
| ATGGCAGCAACGACTGGAAG+GGG | + | chr5.3:6155609-6155628 | MS.gene09922:CDS | 55.0% | |
| CAGACTCAGACTCAGGCACA+AGG | + | chr5.3:6156034-6156053 | MS.gene09922:CDS | 55.0% | |
| CATGGCAGCAACGACTGGAA+GGG | + | chr5.3:6155608-6155627 | MS.gene09922:CDS | 55.0% | |
| CCCGCACGTGCTAATTTCCT+CGG | - | chr5.3:6156245-6156264 | None:intergenic | 55.0% | |
| CCGAGGAAATTAGCACGTGC+GGG | + | chr5.3:6156242-6156261 | MS.gene09922:CDS | 55.0% | |
| GACGCTGTAAGCGTTTCAGG+TGG | - | chr5.3:6155526-6155545 | None:intergenic | 55.0% | |
| GATGAGGTGGCACCAGATCT+TGG | + | chr5.3:6155839-6155858 | MS.gene09922:CDS | 55.0% | |
| GCCGAGGAAATTAGCACGTG+CGG | + | chr5.3:6156241-6156260 | MS.gene09922:CDS | 55.0% | |
| GCGTCTTCTTTCCAAGACGG+CGG | + | chr5.3:6155541-6155560 | MS.gene09922:CDS | 55.0% | |
| TCATGGCAGCAACGACTGGA+AGG | + | chr5.3:6155607-6155626 | MS.gene09922:CDS | 55.0% | |
| TCCAACCCAGCTAGAACGCT+GGG | - | chr5.3:6155873-6155892 | None:intergenic | 55.0% | |
| TGGAGAATACTCCGCCGTCT+TGG | - | chr5.3:6155555-6155574 | None:intergenic | 55.0% | |
| ! | CTGTAAGCGTTTCAGGTGGC+GGG | - | chr5.3:6155522-6155541 | None:intergenic | 55.0% |
| ! | GATGACCCAGCGTTCTAGCT+GGG | + | chr5.3:6155865-6155884 | MS.gene09922:CDS | 55.0% |
| ! | GCTGTAAGCGTTTCAGGTGG+CGG | - | chr5.3:6155523-6155542 | None:intergenic | 55.0% |
| ! | TCATTTGGCGGACCTCGTGT+TGG | + | chr5.3:6156292-6156311 | MS.gene09922:CDS | 55.0% |
| ! | TGTAAGCGTTTCAGGTGGCG+GGG | - | chr5.3:6155521-6155540 | None:intergenic | 55.0% |
| !! | GACGTGATTACAAGGGTGCC+AGG | + | chr5.3:6156382-6156401 | MS.gene09922:CDS | 55.0% |
| ACCGTTACCGGCCATAGCCT+TGG | + | chr5.3:6156196-6156215 | MS.gene09922:CDS | 60.0% | |
| CGCCGGAGGTTGTCATCCAA+GGG | - | chr5.3:6155644-6155663 | None:intergenic | 60.0% | |
| CGCGACACTAGCCCTATTGG+TGG | + | chr5.3:6156219-6156238 | MS.gene09922:CDS | 60.0% | |
| CTAGTGTCGCGCCAAGGCTA+TGG | - | chr5.3:6156210-6156229 | None:intergenic | 60.0% | |
| GCCAAGGCTATGGCCGGTAA+CGG | - | chr5.3:6156200-6156219 | None:intergenic | 60.0% | |
| GGACGCTCAGACTCAGACTC+AGG | + | chr5.3:6156027-6156046 | MS.gene09922:CDS | 60.0% | |
| GGATGACCCAGCGTTCTAGC+TGG | + | chr5.3:6155864-6155883 | MS.gene09922:CDS | 60.0% | |
| GTCTGAGTCTGAGCGTCCTC+GGG | - | chr5.3:6156025-6156044 | None:intergenic | 60.0% | |
| TCGCCGGAGGTTGTCATCCA+AGG | - | chr5.3:6155645-6155664 | None:intergenic | 60.0% | |
| TGGCGCGACACTAGCCCTAT+TGG | + | chr5.3:6156216-6156235 | MS.gene09922:CDS | 60.0% | |
| ! | ACCCAGCGTTCTAGCTGGGT+TGG | + | chr5.3:6155869-6155888 | MS.gene09922:CDS | 60.0% |
| ! | ACTAGCCCTATTGGTGGCCG+AGG | + | chr5.3:6156225-6156244 | MS.gene09922:CDS | 60.0% |
| ! | ATAGGGCTAGTGTCGCGCCA+AGG | - | chr5.3:6156216-6156235 | None:intergenic | 60.0% |
| ! | GTAAGCGTTTCAGGTGGCGG+GGG | - | chr5.3:6155520-6155539 | None:intergenic | 60.0% |
| !! | TAAGCGTTTCAGGTGGCGGG+GGG | - | chr5.3:6155519-6155538 | None:intergenic | 60.0% |
| ACAGCCACGGGTGGCACGTT+TGG | - | chr5.3:6156268-6156287 | None:intergenic | 65.0% | |
| GGATGACAACCTCCGGCGAG+AGG | + | chr5.3:6155646-6155665 | MS.gene09922:CDS | 65.0% | |
| TGTCGCGCCAAGGCTATGGC+CGG | - | chr5.3:6156206-6156225 | None:intergenic | 65.0% | |
| CGGGCCAAACGTGCCACCCG+TGG | + | chr5.3:6156261-6156280 | MS.gene09922:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 6155188 | 6156828 | 6155188 | ID=MS.gene09922 |
| chr5.3 | mRNA | 6155188 | 6156828 | 6155188 | ID=MS.gene09922.t1;Parent=MS.gene09922 |
| chr5.3 | exon | 6155188 | 6156828 | 6155188 | ID=MS.gene09922.t1.exon1;Parent=MS.gene09922.t1 |
| chr5.3 | CDS | 6155188 | 6156828 | 6155188 | ID=cds.MS.gene09922.t1;Parent=MS.gene09922.t1 |
| Gene Sequence |
| Protein sequence |
