AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20122

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20122.t1 MTR_0092s0100 98.688 381 5 0 1 381 352 732 0.0 782
MS.gene20122.t1 MTR_0092s0100 98.688 381 5 0 1 381 349 729 0.0 781
MS.gene20122.t1 MTR_4g026485 63.852 379 129 4 6 381 252 625 3.27e-169 487
MS.gene20122.t1 MTR_8g077940 35.946 370 220 11 22 381 180 542 1.01e-61 207
MS.gene20122.t1 MTR_3g065980 27.895 380 246 7 21 380 169 540 3.45e-37 141
MS.gene20122.t1 MTR_7g027190 26.943 386 249 9 21 381 297 674 2.12e-35 137
MS.gene20122.t1 MTR_3g056110 27.586 377 246 10 23 380 174 542 3.25e-33 130
MS.gene20122.t1 MTR_4g076020 28.533 368 224 13 27 380 87 429 1.49e-31 124
MS.gene20122.t1 MTR_4g077760 29.600 375 240 12 21 380 190 555 1.50e-30 122
MS.gene20122.t1 MTR_5g058860 29.923 391 236 16 21 381 116 498 7.26e-30 120
MS.gene20122.t1 MTR_5g094450 25.469 373 259 7 25 380 162 532 9.80e-30 120
MS.gene20122.t1 MTR_3g089055 26.954 371 252 8 25 380 199 565 1.16e-28 117
MS.gene20122.t1 MTR_3g089055 26.954 371 252 8 25 380 204 570 1.39e-28 117
MS.gene20122.t1 MTR_7g074650 27.322 366 240 9 27 380 441 792 9.46e-28 115
MS.gene20122.t1 MTR_3g072710 26.933 401 237 16 21 381 117 501 9.63e-28 114
MS.gene20122.t1 MTR_2g082090 26.031 388 263 9 10 379 197 578 4.62e-27 113
MS.gene20122.t1 MTR_5g097480 23.099 355 256 7 40 380 193 544 3.77e-26 110
MS.gene20122.t1 MTR_8g093070 26.273 373 254 9 18 379 133 495 8.57e-26 108
MS.gene20122.t1 MTR_1g029420 26.596 376 247 12 25 380 226 592 9.27e-26 109
MS.gene20122.t1 MTR_2g026250 25.065 383 251 12 23 381 227 597 2.13e-24 105
MS.gene20122.t1 MTR_4g104020 24.870 386 255 11 21 381 146 521 4.12e-24 103
MS.gene20122.t1 MTR_4g102790 24.011 379 262 9 21 379 289 661 4.43e-24 104
MS.gene20122.t1 MTR_1g069725 28.533 375 238 16 21 381 106 464 6.93e-24 103
MS.gene20122.t1 MTR_5g019750 30.736 231 147 6 158 381 71 295 1.26e-23 99.8
MS.gene20122.t1 MTR_4g133660 23.622 381 255 9 25 381 176 544 2.09e-23 102
MS.gene20122.t1 MTR_4g097080 22.251 391 273 12 11 380 123 503 7.50e-20 91.3
MS.gene20122.t1 MTR_8g442410 22.798 386 260 10 21 380 152 525 1.29e-19 90.9
MS.gene20122.t1 MTR_2g034280 23.280 378 272 9 20 380 199 575 2.50e-19 90.1
MS.gene20122.t1 MTR_2g097473 23.181 371 262 9 25 379 290 653 3.16e-19 89.7
MS.gene20122.t1 MTR_2g034260 24.870 386 258 12 20 380 206 584 8.21e-19 88.6
MS.gene20122.t1 MTR_2g034250 23.885 381 269 9 20 380 206 585 9.78e-19 88.2
MS.gene20122.t1 MTR_4g064200 23.848 369 263 7 25 379 285 649 1.89e-18 87.4
MS.gene20122.t1 MTR_7g109580 25.201 373 254 11 25 380 190 554 3.84e-18 86.3
MS.gene20122.t1 MTR_1g096030 25.628 398 243 15 21 380 77 459 5.66e-18 85.5
MS.gene20122.t1 MTR_7g062120 22.193 374 259 6 29 380 377 740 4.31e-17 83.6
MS.gene20122.t1 MTR_2g097390 24.129 373 257 11 25 379 276 640 6.17e-17 82.8
MS.gene20122.t1 MTR_2g097310 23.037 382 250 9 25 379 273 637 1.00e-16 82.4
MS.gene20122.t1 MTR_2g099110 23.274 391 251 10 25 380 75 451 1.16e-16 81.6
MS.gene20122.t1 MTR_2g097463 21.563 371 268 7 25 379 291 654 1.24e-16 82.0
MS.gene20122.t1 MTR_2g097410 22.572 381 257 9 25 379 372 740 1.32e-16 82.0
MS.gene20122.t1 MTR_4g064160 21.642 402 275 10 6 379 294 683 7.10e-16 79.7
MS.gene20122.t1 MTR_2g097467 22.372 371 265 9 25 379 290 653 7.56e-16 79.7
MS.gene20122.t1 MTR_2g097380 22.715 361 234 9 32 379 232 560 1.46e-15 78.6
MS.gene20122.t1 MTR_2g089100 22.549 408 245 14 25 379 53 442 4.94e-15 76.6
MS.gene20122.t1 MTR_4g064180 22.432 370 265 9 25 375 255 621 5.77e-15 77.0
MS.gene20122.t1 MTR_4g076140 32.941 170 106 2 1 162 19 188 2.11e-14 74.7
MS.gene20122.t1 MTR_2g097350 23.873 377 253 14 25 379 275 639 4.73e-14 73.9
MS.gene20122.t1 MTR_4g064120 21.409 369 270 7 25 375 255 621 4.97e-14 73.9
MS.gene20122.t1 MTR_4g064150 21.505 372 274 6 25 379 362 732 7.99e-14 73.6
MS.gene20122.t1 MTR_5g015490 21.465 396 251 11 25 380 115 490 2.33e-13 71.6
MS.gene20122.t1 MTR_5g009080 27.179 195 136 2 1 193 33 223 3.62e-13 70.9
MS.gene20122.t1 MTR_3g022830 31.724 145 89 2 25 163 26 166 8.25e-13 69.7
MS.gene20122.t1 MTR_1g086970 23.457 405 269 16 5 380 86 478 1.43e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20122.t1 AT4G00150 58.904 365 134 9 20 381 204 555 1.53e-142 416
MS.gene20122.t1 AT2G45160 51.250 400 158 13 3 381 257 640 1.31e-120 363
MS.gene20122.t1 AT3G60630 51.005 398 167 12 8 381 230 623 1.52e-114 347
MS.gene20122.t1 AT4G36710 32.888 374 225 12 22 380 123 485 9.51e-58 196
MS.gene20122.t1 AT2G01570 29.301 372 240 9 21 380 221 581 2.76e-35 136
MS.gene20122.t1 AT5G66770 27.990 393 260 12 3 380 200 584 7.71e-33 129
MS.gene20122.t1 AT3G03450 27.321 377 246 10 21 380 180 545 8.42e-33 129
MS.gene20122.t1 AT5G17490 28.800 375 237 12 21 380 157 516 2.48e-32 127
MS.gene20122.t1 AT2G04890 26.558 369 249 8 25 380 54 413 6.50e-32 125
MS.gene20122.t1 AT3G54220 28.333 360 245 8 27 379 296 649 2.86e-31 125
MS.gene20122.t1 AT5G48150 24.731 372 260 8 25 380 123 490 1.22e-30 122
MS.gene20122.t1 AT5G48150 24.731 372 260 8 25 380 123 490 1.22e-30 122
MS.gene20122.t1 AT5G48150 24.731 372 260 8 25 380 123 490 1.22e-30 122
MS.gene20122.t1 AT5G48150 24.731 372 260 8 25 380 123 490 1.22e-30 122
MS.gene20122.t1 AT1G14920 26.954 371 250 8 21 380 169 529 4.67e-30 121
MS.gene20122.t1 AT5G41920 26.064 376 247 9 21 380 40 400 1.46e-29 118
MS.gene20122.t1 AT3G50650 28.800 375 222 12 34 380 185 542 4.29e-29 118
MS.gene20122.t1 AT1G66350 25.676 370 250 10 21 380 152 506 8.92e-29 117
MS.gene20122.t1 AT1G50600 24.194 372 261 7 25 380 160 526 5.86e-28 115
MS.gene20122.t1 AT4G08250 29.024 379 243 14 21 379 104 476 1.03e-27 114
MS.gene20122.t1 AT1G50600 24.194 372 261 7 25 380 235 601 1.48e-27 114
MS.gene20122.t1 AT1G50600 24.194 372 261 7 25 380 231 597 1.53e-27 114
MS.gene20122.t1 AT4G17230 25.789 380 245 8 25 380 159 525 3.56e-27 113
MS.gene20122.t1 AT4G17230 25.789 380 245 8 25 380 159 525 3.56e-27 113
MS.gene20122.t1 AT1G21450 23.881 402 276 10 3 380 198 593 1.95e-26 111
MS.gene20122.t1 AT5G59450 23.560 382 255 8 25 380 228 598 4.87e-22 98.2
MS.gene20122.t1 AT1G55580 24.771 327 220 6 70 380 129 445 1.70e-20 93.2
MS.gene20122.t1 AT1G63100 24.495 396 246 14 21 379 249 628 8.02e-20 91.7
MS.gene20122.t1 AT1G63100 24.495 396 246 14 21 379 274 653 8.04e-20 91.7
MS.gene20122.t1 AT1G07520 21.333 375 266 9 29 379 159 528 6.69e-19 88.6
MS.gene20122.t1 AT1G07520 21.333 375 266 9 29 379 159 528 6.69e-19 88.6
MS.gene20122.t1 AT1G07520 21.333 375 266 9 29 379 323 692 9.50e-19 88.6
MS.gene20122.t1 AT1G07520 21.333 375 266 9 29 379 324 693 9.59e-19 88.6
MS.gene20122.t1 AT3G46600 21.594 389 281 7 13 380 194 579 2.72e-17 84.0
MS.gene20122.t1 AT3G46600 21.622 370 273 6 25 380 5 371 3.05e-17 82.8
MS.gene20122.t1 AT3G46600 21.594 389 281 7 13 380 162 547 3.56e-17 83.6
MS.gene20122.t1 AT1G50420 29.897 194 116 5 21 203 54 238 8.09e-16 79.3
MS.gene20122.t1 AT1G07530 22.460 374 257 9 29 379 401 764 8.27e-16 79.7
MS.gene20122.t1 AT4G37650 22.081 394 258 10 25 380 147 529 2.77e-15 77.8
MS.gene20122.t1 AT5G52510 24.522 314 191 11 102 380 338 640 8.26e-14 73.6
MS.gene20122.t1 AT2G29065 21.220 377 273 7 25 379 253 627 1.18e-12 69.7

Find 85 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCAGTTGAGAAGCTTGTTT+TGG 0.246331 7.2:-57026408 MS.gene20122:CDS
TCTTCGCCGCCACCGCTTCC+AGG 0.267033 7.2:+57027304 None:intergenic
GCTTCCCCATAGGAGAAATT+TGG 0.291304 7.2:+57027167 None:intergenic
AAATGCCTCCTTAAAATAAA+AGG 0.302232 7.2:+57027129 None:intergenic
GGAACACGCGCACGGGATAT+TGG 0.339508 7.2:-57027203 MS.gene20122:CDS
TGTTTGGTGCAGAGGATTCC+TGG 0.364499 7.2:-57026281 MS.gene20122:CDS
TTTGCACTCCAGTCTTATTC+CGG 0.364854 7.2:-57026512 MS.gene20122:CDS
CAATCACCAAATTTCTCCTA+TGG 0.395643 7.2:-57027173 MS.gene20122:CDS
ACTTATAGACGCCGGTAATC+CGG 0.404547 7.2:-57027224 MS.gene20122:CDS
TTCTATCCTGCGAGCGCAAA+CGG 0.414907 7.2:+57026384 None:intergenic
GTGCGCGTGTTCCGGATTAC+CGG 0.417521 7.2:+57027213 None:intergenic
GGGAACCCTGGAAGCGGTGG+CGG 0.418143 7.2:-57027310 MS.gene20122:CDS
AGTCTCTGAATTCTGTTTCG+TGG 0.422058 7.2:-57026715 MS.gene20122:CDS
AATATCCCGTGCGCGTGTTC+CGG 0.422409 7.2:+57027205 None:intergenic
AGAGTCGCAGGCAGAGTGTT+TGG 0.429838 7.2:-57026297 MS.gene20122:CDS
AAATTTGAAAACTGCAGAAC+AGG 0.433172 7.2:+57027001 None:intergenic
CCACAGCTTCAAGCAATGCT+TGG 0.439953 7.2:+57026969 None:intergenic
TCGATCATCTGAAGGACATC+CGG 0.441979 7.2:+57026449 None:intergenic
TAATTTGAGAAAGAGCAAAT+AGG 0.442683 7.2:+57026650 None:intergenic
ACTAGCCTCTTGCTCAAGAT+TGG 0.445518 7.2:-57027052 MS.gene20122:CDS
ATCTCATCTCGGTTTCAACT+TGG 0.447156 7.2:-57026199 MS.gene20122:CDS
GGTGCAGAGGATTCCTGGTA+GGG 0.470286 7.2:-57026276 MS.gene20122:CDS
ATCAAGTGATTCAAGCAAAC+CGG 0.470386 7.2:+57026493 None:intergenic
TCGCAATCGCGTAGTGGCTG+CGG 0.474481 7.2:+57026692 None:intergenic
TAAAAGGCAGCCCTTTGAAA+AGG 0.479249 7.2:+57027145 None:intergenic
GCCTAAAATTGTCGTAACAT+TGG 0.480828 7.2:-57026582 MS.gene20122:CDS
CTCGATTTCGTCGTGGTGTG+AGG 0.481816 7.2:+57026817 None:intergenic
CGCAGGATAGAACACTTCCC+TGG 0.482072 7.2:-57026373 MS.gene20122:CDS
AGCTTCAAGCAATGCTTGGT+TGG 0.488113 7.2:+57026973 None:intergenic
TTTGGGCCGTTTGCGCTCGC+AGG 0.491743 7.2:-57026390 MS.gene20122:CDS
TGGTGCAGAGGATTCCTGGT+AGG 0.496504 7.2:-57026277 MS.gene20122:CDS
ACGCGATTGCGAAGCAGTTG+TGG 0.497464 7.2:-57026681 MS.gene20122:CDS
AGCACTAAAGGCAGGTATGA+AGG 0.498213 7.2:+57026626 None:intergenic
ATCTTGAGCAAGAGGCTAGT+TGG 0.505941 7.2:+57027055 None:intergenic
CGAGAAATACTATCTTCAAC+CGG 0.509431 7.2:-57026429 MS.gene20122:CDS
AATCACCAAATTTCTCCTAT+GGG 0.509811 7.2:-57027172 MS.gene20122:CDS
CGGTGGCGGCGAAGATGTAT+CGG 0.512246 7.2:-57027296 MS.gene20122:CDS
CGATCATCTGAAGGACATCC+GGG 0.514286 7.2:+57026450 None:intergenic
ACAATGGTCTTCATTTATGC+AGG 0.517675 7.2:-57026897 MS.gene20122:CDS
GTGCAGAGGATTCCTGGTAG+GGG 0.518621 7.2:-57026275 MS.gene20122:CDS
AAAATCATATGCTGAGGAAA+CGG 0.519993 7.2:+57026533 None:intergenic
AAAACAAGCTTCTCAACTGC+CGG 0.524789 7.2:+57026410 None:intergenic
CCAAGCATTGCTTGAAGCTG+TGG 0.524919 7.2:-57026969 MS.gene20122:CDS
CTTCGCCGCCACCGCTTCCA+GGG 0.526440 7.2:+57027305 None:intergenic
AGAACTCACTTGTCTTGTGT+TGG 0.527376 7.2:-57026232 MS.gene20122:CDS
CACTTGTCTTGTGTTGGCAA+AGG 0.532124 7.2:-57026226 MS.gene20122:CDS
ATGGGGAACCCTGGAAGCGG+TGG 0.534973 7.2:-57027313 MS.gene20122:CDS
TGTCTTGTGTTGGCAAAGGA+AGG 0.536796 7.2:-57026222 MS.gene20122:CDS
TAGTAACTTCACAGAGTCGC+AGG 0.538196 7.2:-57026309 MS.gene20122:CDS
CAAGCAAACCGGAATAAGAC+TGG 0.539954 7.2:+57026504 None:intergenic
TCCACATGGAATCCCCTACC+AGG 0.543648 7.2:+57026263 None:intergenic
AAGCACCAATCTTGAGCAAG+AGG 0.549041 7.2:+57027047 None:intergenic
CTTTGAAAAGGCTTCCCCAT+AGG 0.549307 7.2:+57027157 None:intergenic
ATCGGCTCATCAGTTTCAGC+AGG 0.556576 7.2:-57027278 MS.gene20122:CDS
TTCACGAATCGAAGCACTAA+AGG 0.557184 7.2:+57026614 None:intergenic
TCGATTTCGTCGTGGTGTGA+GGG 0.560923 7.2:+57026818 None:intergenic
AGGCAGAGTGTTTGGTGCAG+AGG 0.564913 7.2:-57026289 MS.gene20122:CDS
GCACTAAAGGCAGGTATGAA+GGG 0.565237 7.2:+57026627 None:intergenic
AAGCAGAAAATGGGGAACCC+TGG 0.568423 7.2:-57027322 None:intergenic
GAGAATCTGAAGCAATATGC+TGG 0.570600 7.2:-57026779 MS.gene20122:CDS
GGTAATCCGGAACACGCGCA+CGG 0.575492 7.2:-57027211 MS.gene20122:CDS
AAAACCTACTCTTGTCATCA+GGG 0.582681 7.2:-57026349 MS.gene20122:CDS
GCATTGCTTGAAGCTGTGGA+AGG 0.584725 7.2:-57026965 MS.gene20122:CDS
CGAATCGAAGCACTAAAGGC+AGG 0.590451 7.2:+57026618 None:intergenic
GTAATCCGGAACACGCGCAC+GGG 0.591224 7.2:-57027210 MS.gene20122:CDS
GCAAAGGAAGGATCTCATCT+CGG 0.596929 7.2:-57026210 MS.gene20122:CDS
AGTATTTCTCGATCATCTGA+AGG 0.598913 7.2:+57026441 None:intergenic
GGATAGGAGTTGTGATCGAA+CGG 0.600755 7.2:-57026561 MS.gene20122:CDS
TTATGCAGGAGTTAGCACTA+AGG 0.606201 7.2:-57026883 MS.gene20122:CDS
AGTTCTGCTTCTTCTCCACA+TGG 0.609894 7.2:+57026249 None:intergenic
AAAATGGGGAACCCTGGAAG+CGG 0.610138 7.2:-57027316 MS.gene20122:CDS
AAATTGTCGTAACATTGGAT+AGG 0.615531 7.2:-57026577 MS.gene20122:CDS
ACGCGCACGGGATATTGGCG+CGG 0.617795 7.2:-57027198 MS.gene20122:CDS
AGAACCCTGATGACAAGAGT+AGG 0.623547 7.2:+57026345 None:intergenic
CAGGAGTTAGCACTAAGGAA+TGG 0.623926 7.2:-57026878 MS.gene20122:CDS
GTGAAGTTACTAAATGTCAA+CGG 0.630606 7.2:+57026320 None:intergenic
ATCACCAAATTTCTCCTATG+GGG 0.632427 7.2:-57027171 MS.gene20122:CDS
TCCTGGTAGGGGATTCCATG+TGG 0.639151 7.2:-57026264 MS.gene20122:CDS
ACTGCTTCGCAATCGCGTAG+TGG 0.643756 7.2:+57026686 None:intergenic
AATGCAGTGATCTTGAGTGC+AGG 0.644744 7.2:+57026848 None:intergenic
GAGTTAGCACTAAGGAATGG+TGG 0.649571 7.2:-57026875 MS.gene20122:CDS
TGTTAGTGTTAATGTCCACC+CGG 0.680851 7.2:-57026468 MS.gene20122:CDS
GCTGCAGAACTTATAGACGC+CGG 0.689078 7.2:-57027232 MS.gene20122:CDS
AACTAAGCTCGATTTCGTCG+TGG 0.700452 7.2:+57026810 None:intergenic
TCATCTGAAGGACATCCGGG+TGG 0.719806 7.2:+57026453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATATAATAGATTTTGATAT+TGG - chr7.2:57026574-57026593 MS.gene20122:CDS 15.0%
!!! ATAGATTTTGATATTGGTTT+TGG - chr7.2:57026580-57026599 MS.gene20122:CDS 20.0%
! AAATGCCTCCTTAAAATAAA+AGG + chr7.2:57026377-57026396 None:intergenic 25.0%
! TAATTTGAGAAAGAGCAAAT+AGG + chr7.2:57026856-57026875 None:intergenic 25.0%
!! TATAATTGATCAGCTTTTCA+AGG - chr7.2:57026249-57026268 MS.gene20122:CDS 25.0%
AAAATCATATGCTGAGGAAA+CGG + chr7.2:57026973-57026992 None:intergenic 30.0%
AAATTGTCGTAACATTGGAT+AGG - chr7.2:57026926-57026945 MS.gene20122:CDS 30.0%
AAATTTGAAAACTGCAGAAC+AGG + chr7.2:57026505-57026524 None:intergenic 30.0%
AATCACCAAATTTCTCCTAT+GGG - chr7.2:57026331-57026350 MS.gene20122:CDS 30.0%
GTGAAGTTACTAAATGTCAA+CGG + chr7.2:57027186-57027205 None:intergenic 30.0%
! TCCAATGTTACGACAATTTT+AGG + chr7.2:57026925-57026944 None:intergenic 30.0%
!!! GATTTTGATATTGGTTTTGG+TGG - chr7.2:57026583-57026602 MS.gene20122:CDS 30.0%
AAAAACCTACTCTTGTCATC+AGG - chr7.2:57027153-57027172 MS.gene20122:CDS 35.0%
AAAACCTACTCTTGTCATCA+GGG - chr7.2:57027154-57027173 MS.gene20122:CDS 35.0%
ACAATGGTCTTCATTTATGC+AGG - chr7.2:57026606-57026625 MS.gene20122:CDS 35.0%
AGTATTTCTCGATCATCTGA+AGG + chr7.2:57027065-57027084 None:intergenic 35.0%
AGTGCAAAAATCATATGCTG+AGG + chr7.2:57026979-57026998 None:intergenic 35.0%
ATCAAGTGATTCAAGCAAAC+CGG + chr7.2:57027013-57027032 None:intergenic 35.0%
ATCACCAAATTTCTCCTATG+GGG - chr7.2:57026332-57026351 MS.gene20122:CDS 35.0%
CAATCACCAAATTTCTCCTA+TGG - chr7.2:57026330-57026349 MS.gene20122:CDS 35.0%
CGAGAAATACTATCTTCAAC+CGG - chr7.2:57027074-57027093 MS.gene20122:CDS 35.0%
GCCTAAAATTGTCGTAACAT+TGG - chr7.2:57026921-57026940 MS.gene20122:CDS 35.0%
!!! AAGGGCTGCCTTTTATTTTA+AGG - chr7.2:57026366-57026385 MS.gene20122:CDS 35.0%
!!! ATATTGGTTTTGGTGGACAA+TGG - chr7.2:57026590-57026609 MS.gene20122:CDS 35.0%
AAAACAAGCTTCTCAACTGC+CGG + chr7.2:57027096-57027115 None:intergenic 40.0%
AGAACTCACTTGTCTTGTGT+TGG - chr7.2:57027271-57027290 MS.gene20122:CDS 40.0%
AGTCTCTGAATTCTGTTTCG+TGG - chr7.2:57026788-57026807 MS.gene20122:CDS 40.0%
ATCTCATCTCGGTTTCAACT+TGG - chr7.2:57027304-57027323 MS.gene20122:CDS 40.0%
TGTTAGTGTTAATGTCCACC+CGG - chr7.2:57027035-57027054 MS.gene20122:CDS 40.0%
TTATGCAGGAGTTAGCACTA+AGG - chr7.2:57026620-57026639 MS.gene20122:CDS 40.0%
! GACAAGAGTAGGTTTTTCCA+GGG + chr7.2:57027150-57027169 None:intergenic 40.0%
! GAGAATCTGAAGCAATATGC+TGG - chr7.2:57026724-57026743 MS.gene20122:CDS 40.0%
! TAAAAGGCAGCCCTTTGAAA+AGG + chr7.2:57026361-57026380 None:intergenic 40.0%
! TATGGGGAAGCCTTTTCAAA+GGG - chr7.2:57026348-57026367 MS.gene20122:CDS 40.0%
! TGACAAGAGTAGGTTTTTCC+AGG + chr7.2:57027151-57027170 None:intergenic 40.0%
! TTCACGAATCGAAGCACTAA+AGG + chr7.2:57026892-57026911 None:intergenic 40.0%
!! GCAGTTGAGAAGCTTGTTTT+GGG - chr7.2:57027096-57027115 MS.gene20122:CDS 40.0%
!! TTTGCACTCCAGTCTTATTC+CGG - chr7.2:57026991-57027010 MS.gene20122:CDS 40.0%
!!! GGCTGCCTTTTATTTTAAGG+AGG - chr7.2:57026369-57026388 MS.gene20122:CDS 40.0%
AACTAAGCTCGATTTCGTCG+TGG + chr7.2:57026696-57026715 None:intergenic 45.0%
AAGCACCAATCTTGAGCAAG+AGG + chr7.2:57026459-57026478 None:intergenic 45.0%
AATGCAGTGATCTTGAGTGC+AGG + chr7.2:57026658-57026677 None:intergenic 45.0%
ACTTATAGACGCCGGTAATC+CGG - chr7.2:57026279-57026298 MS.gene20122:CDS 45.0%
AGAACCCTGATGACAAGAGT+AGG + chr7.2:57027161-57027180 None:intergenic 45.0%
AGCACTAAAGGCAGGTATGA+AGG + chr7.2:57026880-57026899 None:intergenic 45.0%
AGCTTCAAGCAATGCTTGGT+TGG + chr7.2:57026533-57026552 None:intergenic 45.0%
AGTTCTGCTTCTTCTCCACA+TGG + chr7.2:57027257-57027276 None:intergenic 45.0%
ATCTTGAGCAAGAGGCTAGT+TGG + chr7.2:57026451-57026470 None:intergenic 45.0%
CAAGCAAACCGGAATAAGAC+TGG + chr7.2:57027002-57027021 None:intergenic 45.0%
CAGGAGTTAGCACTAAGGAA+TGG - chr7.2:57026625-57026644 MS.gene20122:CDS 45.0%
GAGTTAGCACTAAGGAATGG+TGG - chr7.2:57026628-57026647 MS.gene20122:CDS 45.0%
GCAAAGGAAGGATCTCATCT+CGG - chr7.2:57027293-57027312 MS.gene20122:CDS 45.0%
GCACTAAAGGCAGGTATGAA+GGG + chr7.2:57026879-57026898 None:intergenic 45.0%
GCTTCCCCATAGGAGAAATT+TGG + chr7.2:57026339-57026358 None:intergenic 45.0%
GGATAGGAGTTGTGATCGAA+CGG - chr7.2:57026942-57026961 MS.gene20122:CDS 45.0%
TAGTAACTTCACAGAGTCGC+AGG - chr7.2:57027194-57027213 MS.gene20122:CDS 45.0%
TCGATCATCTGAAGGACATC+CGG + chr7.2:57027057-57027076 None:intergenic 45.0%
! ACTAGCCTCTTGCTCAAGAT+TGG - chr7.2:57026451-57026470 MS.gene20122:CDS 45.0%
! CACTTGTCTTGTGTTGGCAA+AGG - chr7.2:57027277-57027296 MS.gene20122:CDS 45.0%
! CTATGGGGAAGCCTTTTCAA+AGG - chr7.2:57026347-57026366 MS.gene20122:CDS 45.0%
! CTTTGAAAAGGCTTCCCCAT+AGG + chr7.2:57026349-57026368 None:intergenic 45.0%
! TGTCTTGTGTTGGCAAAGGA+AGG - chr7.2:57027281-57027300 MS.gene20122:CDS 45.0%
!! GGCAGTTGAGAAGCTTGTTT+TGG - chr7.2:57027095-57027114 MS.gene20122:CDS 45.0%
CCACAGCTTCAAGCAATGCT+TGG + chr7.2:57026537-57026556 None:intergenic 50.0%
CGATCATCTGAAGGACATCC+GGG + chr7.2:57027056-57027075 None:intergenic 50.0%
GCATTGCTTGAAGCTGTGGA+AGG - chr7.2:57026538-57026557 MS.gene20122:CDS 50.0%
GCTGCAGAACTTATAGACGC+CGG - chr7.2:57026271-57026290 MS.gene20122:CDS 50.0%
TCGATTTCGTCGTGGTGTGA+GGG + chr7.2:57026688-57026707 None:intergenic 50.0%
TTCTATCCTGCGAGCGCAAA+CGG + chr7.2:57027122-57027141 None:intergenic 50.0%
! ATCGGCTCATCAGTTTCAGC+AGG - chr7.2:57026225-57026244 MS.gene20122:CDS 50.0%
! CCAAGCATTGCTTGAAGCTG+TGG - chr7.2:57026534-57026553 MS.gene20122:CDS 50.0%
! CGAATCGAAGCACTAAAGGC+AGG + chr7.2:57026888-57026907 None:intergenic 50.0%
!! TGTTTGGTGCAGAGGATTCC+TGG - chr7.2:57027222-57027241 MS.gene20122:CDS 50.0%
AATATCCCGTGCGCGTGTTC+CGG + chr7.2:57026301-57026320 None:intergenic 55.0%
ACGCGATTGCGAAGCAGTTG+TGG - chr7.2:57026822-57026841 MS.gene20122:CDS 55.0%
ACTGCTTCGCAATCGCGTAG+TGG + chr7.2:57026820-57026839 None:intergenic 55.0%
CGCAGGATAGAACACTTCCC+TGG - chr7.2:57027130-57027149 MS.gene20122:CDS 55.0%
CTCGATTTCGTCGTGGTGTG+AGG + chr7.2:57026689-57026708 None:intergenic 55.0%
GGTGCAGAGGATTCCTGGTA+GGG - chr7.2:57027227-57027246 MS.gene20122:CDS 55.0%
GTGCAGAGGATTCCTGGTAG+GGG - chr7.2:57027228-57027247 MS.gene20122:CDS 55.0%
TCATCTGAAGGACATCCGGG+TGG + chr7.2:57027053-57027072 None:intergenic 55.0%
TCCACATGGAATCCCCTACC+AGG + chr7.2:57027243-57027262 None:intergenic 55.0%
TCCTGGTAGGGGATTCCATG+TGG - chr7.2:57027239-57027258 MS.gene20122:CDS 55.0%
TGGTGCAGAGGATTCCTGGT+AGG - chr7.2:57027226-57027245 MS.gene20122:CDS 55.0%
! AGAGTCGCAGGCAGAGTGTT+TGG - chr7.2:57027206-57027225 MS.gene20122:CDS 55.0%
!! AGGCAGAGTGTTTGGTGCAG+AGG - chr7.2:57027214-57027233 MS.gene20122:CDS 55.0%
GGAACACGCGCACGGGATAT+TGG - chr7.2:57026300-57026319 MS.gene20122:CDS 60.0%
GGTAATCCGGAACACGCGCA+CGG - chr7.2:57026292-57026311 MS.gene20122:CDS 60.0%
GTAATCCGGAACACGCGCAC+GGG - chr7.2:57026293-57026312 MS.gene20122:CDS 60.0%
GTGCGCGTGTTCCGGATTAC+CGG + chr7.2:57026293-57026312 None:intergenic 60.0%
TCGCAATCGCGTAGTGGCTG+CGG + chr7.2:57026814-57026833 None:intergenic 60.0%
! CGGTGGCGGCGAAGATGTAT+CGG - chr7.2:57026207-57026226 MS.gene20122:CDS 60.0%
ACGCGCACGGGATATTGGCG+CGG - chr7.2:57026305-57026324 MS.gene20122:CDS 65.0%
ATGGGGAACCCTGGAAGCGG+TGG - chr7.2:57026190-57026209 MS.gene20122:CDS 65.0%
TTTGGGCCGTTTGCGCTCGC+AGG - chr7.2:57027113-57027132 MS.gene20122:CDS 65.0%
CTTCGCCGCCACCGCTTCCA+GGG + chr7.2:57026201-57026220 None:intergenic 70.0%
GGGAACCCTGGAAGCGGTGG+CGG - chr7.2:57026193-57026212 MS.gene20122:CDS 70.0%
TCTTCGCCGCCACCGCTTCC+AGG + chr7.2:57026202-57026221 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 57026190 57027335 57026190 ID=MS.gene20122
chr7.2 mRNA 57026190 57027335 57026190 ID=MS.gene20122.t1;Parent=MS.gene20122
chr7.2 exon 57026190 57027335 57026190 ID=MS.gene20122.t1.exon1;Parent=MS.gene20122.t1
chr7.2 CDS 57026190 57027335 57026190 ID=cds.MS.gene20122.t1;Parent=MS.gene20122.t1
Gene Sequence

>MS.gene20122

ATGGGGAACCCTGGAAGCGGTGGCGGCGAAGATGTATCGGCTCATCAGTTTCAGCAGGCTATAATTGATCAGCTTTTCAAGGCTGCAGAACTTATAGACGCCGGTAATCCGGAACACGCGCACGGGATATTGGCGCGGCTCAATCACCAAATTTCTCCTATGGGGAAGCCTTTTCAAAGGGCTGCCTTTTATTTTAAGGAGGCATTTCAATTGCTTCTTCAATCAAATGTCAACAACAGCAACAACAACAGCTTTTCACCAACTAGCCTCTTGCTCAAGATTGGTGCTTATAAATCATTCTCAGAAATCTCACCTGTTCTGCAGTTTTCAAATTTCACTTCCAACCAAGCATTGCTTGAAGCTGTGGAAGGTTTCGATCGAATTCATATAATAGATTTTGATATTGGTTTTGGTGGACAATGGTCTTCATTTATGCAGGAGTTAGCACTAAGGAATGGTGGTGCACCTGCACTCAAGATCACTGCATTTGTTTCGCCCTCACACCACGACGAAATCGAGCTTAGTTTCACTAACGAGAATCTGAAGCAATATGCTGGTGAAATTAACATGTCATTTGAGCTCGAAATTCTGACACTTGAGTCTCTGAATTCTGTTTCGTGGCCGCAGCCACTACGCGATTGCGAAGCAGTTGTGGTTAACTTGCCTATTTGCTCTTTCTCAAATTACCCTTCATACCTGCCTTTAGTGCTTCGATTCGTGAAGCAGCTAATGCCTAAAATTGTCGTAACATTGGATAGGAGTTGTGATCGAACGGATGCACCGTTTCCTCAGCATATGATTTTTGCACTCCAGTCTTATTCCGGTTTGCTTGAATCACTTGATGCTGTTAGTGTTAATGTCCACCCGGATGTCCTTCAGATGATCGAGAAATACTATCTTCAACCGGCAGTTGAGAAGCTTGTTTTGGGCCGTTTGCGCTCGCAGGATAGAACACTTCCCTGGAAAAACCTACTCTTGTCATCAGGGTTCTCGCCGTTGACATTTAGTAACTTCACAGAGTCGCAGGCAGAGTGTTTGGTGCAGAGGATTCCTGGTAGGGGATTCCATGTGGAGAAGAAGCAGAACTCACTTGTCTTGTGTTGGCAAAGGAAGGATCTCATCTCGGTTTCAACTTGGAGATGCTGA
Protein sequence

>MS.gene20122.t1

MGNPGSGGGEDVSAHQFQQAIIDQLFKAAELIDAGNPEHAHGILARLNHQISPMGKPFQRAAFYFKEAFQLLLQSNVNNSNNNSFSPTSLLLKIGAYKSFSEISPVLQFSNFTSNQALLEAVEGFDRIHIIDFDIGFGGQWSSFMQELALRNGGAPALKITAFVSPSHHDEIELSFTNENLKQYAGEINMSFELEILTLESLNSVSWPQPLRDCEAVVVNLPICSFSNYPSYLPLVLRFVKQLMPKIVVTLDRSCDRTDAPFPQHMIFALQSYSGLLESLDAVSVNVHPDVLQMIEKYYLQPAVEKLVLGRLRSQDRTLPWKNLLLSSGFSPLTFSNFTESQAECLVQRIPGRGFHVEKKQNSLVLCWQRKDLISVSTWRC