AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20170.t1 MTR_8g101570 93.493 292 13 1 1 292 1 286 0.0 555
MS.gene20170.t1 MTR_6g092660 59.333 300 108 4 1 293 1 293 1.07e-121 350
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20170.t1 AT2G19570 57.003 307 108 5 1 292 1 298 7.40e-117 338
MS.gene20170.t1 AT4G29600 49.020 306 133 7 2 295 5 299 2.63e-90 271
MS.gene20170.t1 AT4G29610 50.365 274 120 5 28 292 21 287 8.73e-89 266
MS.gene20170.t1 AT4G29620 45.395 304 139 7 2 295 26 312 5.15e-78 241
MS.gene20170.t1 AT4G29640 43.206 287 147 7 16 292 13 293 2.48e-64 206
MS.gene20170.t1 AT4G29630 48.918 231 97 5 2 222 4 223 4.44e-62 196
MS.gene20170.t1 AT4G29570 42.446 278 141 7 26 292 23 292 5.23e-58 188
MS.gene20170.t1 AT4G29650 38.062 289 127 7 6 292 7 245 7.79e-49 163
MS.gene20170.t1 AT4G29580 39.726 292 151 10 4 282 7 286 1.01e-48 164
MS.gene20170.t1 AT4G29580 39.726 292 151 10 4 282 7 286 1.20e-47 165

Find 112 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGACAGAGAAGTGAAATT+TGG 0.221554 4.4:-3938152 None:intergenic
GACAGCTCCGACGTGGAAAT+TGG 0.275635 4.4:-3937895 None:intergenic
CGGGTGCTCCGCGGATTTCT+TGG 0.278140 4.4:-3938097 None:intergenic
TCCTAGTTTGGGTCCTCTTC+AGG 0.279807 4.4:+3938457 MS.gene20170:CDS
TGAAGATTTGGCCATGGATT+TGG 0.298830 4.4:-3937802 None:intergenic
TGGGTGGTCGGGCGAGGGAT+TGG 0.308461 4.4:-3937869 None:intergenic
AAAGTCTGCAATTGACATTT+AGG 0.308869 4.4:-3938611 None:intergenic
GGTAATGATGATTTGAATAT+CGG 0.323221 4.4:-3938117 None:intergenic
GGGTGGTCGGGCGAGGGATT+GGG 0.334841 4.4:-3937868 None:intergenic
GGCGGCGTTTAATCCTAGTT+TGG 0.341522 4.4:+3938445 MS.gene20170:CDS
CCAGATGGTGAATTACTATA+AGG 0.358174 4.4:-3938368 None:intergenic
GAGAGTGAGGGATGAAGATT+TGG 0.361013 4.4:-3937814 None:intergenic
TCCTCTTCAGGCTGCGGTGG+TGG 0.366749 4.4:+3938469 MS.gene20170:CDS
GCGGCGTTTAATCCTAGTTT+GGG 0.380242 4.4:+3938446 MS.gene20170:CDS
CCTTATAGTAATTCACCATC+TGG 0.384670 4.4:+3938368 MS.gene20170:CDS
GAGACAGAGAAGTGAAATTT+GGG 0.388256 4.4:-3938151 None:intergenic
GGATTTCTTGGAAGAATTGC+CGG 0.395040 4.4:-3938085 None:intergenic
GAATTGCCGGCAGTGGCCAC+AGG 0.404218 4.4:-3938072 None:intergenic
CCGGCAGTGGCCACAGGGAG+CGG 0.421277 4.4:-3938066 None:intergenic
GCGTTTATTGTCGCCGGCGG+CGG 0.423175 4.4:+3938491 MS.gene20170:CDS
GATAGTTTCTCTGTTGTTAA+CGG 0.423842 4.4:-3938266 None:intergenic
GACGGCAAATGAGTGGAGAT+TGG 0.429471 4.4:-3938039 None:intergenic
TGACAAGTGATGGGAGGAGT+TGG 0.430025 4.4:-3937839 None:intergenic
GGAGGAGTTGGGTGAGAGTG+AGG 0.430382 4.4:-3937827 None:intergenic
TTTGGCCATGGATTTGGCTT+CGG 0.430920 4.4:-3937796 None:intergenic
CCATCGGGTCGGATCTTAAT+CGG 0.432605 4.4:+3937927 MS.gene20170:CDS
TGGAGATGGGTGGTCGGGCG+AGG 0.432898 4.4:-3937875 None:intergenic
TCGGGCGAGGGATTGGGCGG+AGG 0.439797 4.4:-3937862 None:intergenic
AGTGAAATTTGGGTCAGATT+CGG 0.443529 4.4:-3938141 None:intergenic
GAAGCTAAAGATTGCGGCTA+TGG 0.452033 4.4:+3938322 MS.gene20170:CDS
GAAATTGGAGATGGGTGGTC+GGG 0.455841 4.4:-3937880 None:intergenic
CCGCTCCCTGTGGCCACTGC+CGG 0.461973 4.4:+3938066 MS.gene20170:CDS
AGTGATGAAGAGGGAGGCCT+GGG 0.468579 4.4:-3937965 None:intergenic
TAAAGGATCTTATGTTGAAT+CGG 0.470270 4.4:+3938424 MS.gene20170:CDS
CTTGGAAGAATTGCCGGCAG+TGG 0.473200 4.4:-3938079 None:intergenic
TGGGCGGAGGTGACAAGTGA+TGG 0.473273 4.4:-3937849 None:intergenic
GACAAGTGATGGGAGGAGTT+GGG 0.474173 4.4:-3937838 None:intergenic
GAGAAACTGTTCGGCGTGGA+TGG 0.474486 4.4:-3937988 None:intergenic
ACGGCAAATGAGTGGAGATT+GGG 0.480319 4.4:-3938038 None:intergenic
GAGTGATGAAGAGGGAGGCC+TGG 0.482748 4.4:-3937966 None:intergenic
ATCACCGTTGCAAACGCCAT+TGG 0.486585 4.4:-3938288 None:intergenic
ACCATCTGGGATGGCGATTG+TGG 0.487559 4.4:+3938382 MS.gene20170:CDS
GCCACAGGGAGCGGCGGAGA+CGG 0.489161 4.4:-3938057 None:intergenic
GGCCATGGATTTGGCTTCGG+TGG 0.493035 4.4:-3937793 None:intergenic
AATTTCCACGTCGGAGCTGT+CGG 0.496820 4.4:+3937897 MS.gene20170:CDS
AAAACAGAGGAGCGTGTTGA+GGG 0.498221 4.4:-3938211 None:intergenic
CCGATTAAGATCCGACCCGA+TGG 0.499542 4.4:-3937927 None:intergenic
CGGAAGAAGCTTTAACCCGT+TGG 0.502023 4.4:-3938246 None:intergenic
GCCACCACCGCAGCCTGAAG+AGG 0.504864 4.4:-3938470 None:intergenic
GGGTCCTCTTCAGGCTGCGG+TGG 0.509491 4.4:+3938466 MS.gene20170:CDS
TGGTCGGGCGAGGGATTGGG+CGG 0.511129 4.4:-3937865 None:intergenic
GTAATGATGATTTGAATATC+GGG 0.511400 4.4:-3938116 None:intergenic
CGATTAAGATCCGACCCGAT+GGG 0.512765 4.4:-3937926 None:intergenic
GGAGATGGGTGGTCGGGCGA+GGG 0.514417 4.4:-3937874 None:intergenic
ATTTGTGAGGAGAAACTGTT+CGG 0.519896 4.4:-3937997 None:intergenic
GGAAATTGGAGATGGGTGGT+CGG 0.522634 4.4:-3937881 None:intergenic
GTGGTGGCGTTTATTGTCGC+CGG 0.545550 4.4:+3938485 MS.gene20170:CDS
TTTGGGTCCTCTTCAGGCTG+CGG 0.545667 4.4:+3938463 MS.gene20170:CDS
TCCGACGTGGAAATTGGAGA+TGG 0.546314 4.4:-3937889 None:intergenic
TCGTGTGGTCCAAATCTGTA+AGG 0.546455 4.4:-3938182 None:intergenic
GAGGGATGAAGATTTGGCCA+TGG 0.547112 4.4:-3937808 None:intergenic
GAGGAGTTGGGTGAGAGTGA+GGG 0.549562 4.4:-3937826 None:intergenic
GGCCATTGATCATTCATCAA+AGG 0.550701 4.4:-3938642 None:intergenic
GAGATTGGGTTCGTCGTGGA+GGG 0.551352 4.4:-3938024 None:intergenic
GGGCGGAGGTGACAAGTGAT+GGG 0.554215 4.4:-3937848 None:intergenic
TGAGATCGTGGGTGCTGTGT+TGG 0.554657 4.4:+3938523 MS.gene20170:CDS
AGCTGTCGGATTATCCCCAT+CGG 0.556466 4.4:+3937911 MS.gene20170:CDS
GCTAAAGATTGCGGCTATGG+AGG 0.558173 4.4:+3938325 MS.gene20170:CDS
ATGGCGATTGTGGATTGTAA+TGG 0.559387 4.4:+3938392 MS.gene20170:CDS
GATCGTGGGTGCTGTGTTGG+TGG 0.562554 4.4:+3938526 MS.gene20170:CDS
AGCTTTAACCCGTTGGTGCG+TGG 0.562632 4.4:-3938239 None:intergenic
GGAGTCAATGTCGAATTCCC+AGG 0.562922 4.4:+3937948 MS.gene20170:CDS
GGAGATTGGGTTCGTCGTGG+AGG 0.563710 4.4:-3938025 None:intergenic
CGGCGGAGACGGCAAATGAG+TGG 0.568502 4.4:-3938046 None:intergenic
CGGTAAGTATGATGAGATCG+TGG 0.575614 4.4:+3938511 MS.gene20170:CDS
CTTATAGTAATTCACCATCT+GGG 0.578783 4.4:+3938369 MS.gene20170:CDS
AGCCACCGAAGCCAAATCCA+TGG 0.582069 4.4:+3937791 MS.gene20170:CDS
CGTGGATGGAGTGATGAAGA+GGG 0.588357 4.4:-3937974 None:intergenic
CTATCCAATGGCGTTTGCAA+CGG 0.589084 4.4:+3938284 MS.gene20170:CDS
GCCTGAAGAGGACCCAAACT+AGG 0.589128 4.4:-3938458 None:intergenic
CTCATCATACTTACCGCCGC+CGG 0.591519 4.4:-3938504 None:intergenic
CCGACGTGGAAATTGGAGAT+GGG 0.591562 4.4:-3937888 None:intergenic
GAAATTTGGGTCAGATTCGG+AGG 0.592488 4.4:-3938138 None:intergenic
TAGTAATTCACCATCTGGGA+TGG 0.593503 4.4:+3938373 MS.gene20170:CDS
GCCGTCTCCGCCGCTCCCTG+TGG 0.593913 4.4:+3938056 MS.gene20170:CDS
GCGTGGATGGAGTGATGAAG+AGG 0.599358 4.4:-3937975 None:intergenic
GCTGTCGGATTATCCCCATC+GGG 0.602496 4.4:+3937912 MS.gene20170:CDS
GCAGTGGCCACAGGGAGCGG+CGG 0.603868 4.4:-3938063 None:intergenic
AATTGCCGGCAGTGGCCACA+GGG 0.607932 4.4:-3938071 None:intergenic
CGGAGGTGACAAGTGATGGG+AGG 0.610703 4.4:-3937845 None:intergenic
TCCACAATCGCCATCCCAGA+TGG 0.613085 4.4:-3938383 None:intergenic
CGGAGCTATGGTGAAACAAG+AGG 0.615878 4.4:+3938556 MS.gene20170:CDS
GGTAAGTATGATGAGATCGT+GGG 0.617309 4.4:+3938512 MS.gene20170:CDS
GATTAAGATCCGACCCGATG+GGG 0.619338 4.4:-3937925 None:intergenic
GGTGGAGAAAGACGGAGCTA+TGG 0.623714 4.4:+3938544 MS.gene20170:CDS
TCGGATTATCCCCATCGGGT+CGG 0.633245 4.4:+3937916 MS.gene20170:CDS
GTGGCGTTTATTGTCGCCGG+CGG 0.634221 4.4:+3938488 MS.gene20170:CDS
GGAGCTATGGTGAAACAAGA+GGG 0.637476 4.4:+3938557 MS.gene20170:CDS
TCATGAGAAGCTAAAGATTG+CGG 0.637485 4.4:+3938316 MS.gene20170:CDS
TTTGAATATCGGGTGCTCCG+CGG 0.650008 4.4:-3938106 None:intergenic
AGTGGAGATTGGGTTCGTCG+TGG 0.655961 4.4:-3938028 None:intergenic
ACGTGGAAATTGGAGATGGG+TGG 0.657179 4.4:-3937885 None:intergenic
ACAACAGAGAAACTATCCAA+TGG 0.665950 4.4:+3938272 MS.gene20170:CDS
AGGATCTTATGTTGAATCGG+CGG 0.667327 4.4:+3938427 MS.gene20170:CDS
CAATTCTTCCAAGAAATCCG+CGG 0.668096 4.4:+3938089 MS.gene20170:CDS
ATAATCCGACAGCTCCGACG+TGG 0.676269 4.4:-3937902 None:intergenic
CCCATCTCCAATTTCCACGT+CGG 0.677245 4.4:+3937888 MS.gene20170:CDS
GCTGTGTTGGTGGAGAAAGA+CGG 0.682938 4.4:+3938536 MS.gene20170:CDS
TGAGGAGAAACTGTTCGGCG+TGG 0.685610 4.4:-3937992 None:intergenic
CGTGGAGGGAGAGATTTGTG+AGG 0.692173 4.4:-3938010 None:intergenic
GGATGGAGTGATGAAGAGGG+AGG 0.695700 4.4:-3937971 None:intergenic
TGTTGAGGGAGAAGATCGTG+TGG 0.703420 4.4:-3938197 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTAATGGAAAAATCTATAA+AGG + chr4.4:3938407-3938426 MS.gene20170:CDS 20.0%
! GGTAATGATGATTTGAATAT+CGG - chr4.4:3938120-3938139 None:intergenic 25.0%
! GTAATGATGATTTGAATATC+GGG - chr4.4:3938119-3938138 None:intergenic 25.0%
! TAAAGGATCTTATGTTGAAT+CGG + chr4.4:3938424-3938443 MS.gene20170:CDS 25.0%
AAAGTCTGCAATTGACATTT+AGG - chr4.4:3938614-3938633 None:intergenic 30.0%
CTTATAGTAATTCACCATCT+GGG + chr4.4:3938369-3938388 MS.gene20170:CDS 30.0%
GATAGTTTCTCTGTTGTTAA+CGG - chr4.4:3938269-3938288 None:intergenic 30.0%
! CATTTTCTTCCTTACAGATT+TGG + chr4.4:3938173-3938192 MS.gene20170:CDS 30.0%
ACAACAGAGAAACTATCCAA+TGG + chr4.4:3938272-3938291 MS.gene20170:CDS 35.0%
AGTGAAATTTGGGTCAGATT+CGG - chr4.4:3938144-3938163 None:intergenic 35.0%
ATTTGTGAGGAGAAACTGTT+CGG - chr4.4:3938000-3938019 None:intergenic 35.0%
CCAGATGGTGAATTACTATA+AGG - chr4.4:3938371-3938390 None:intergenic 35.0%
CCTTATAGTAATTCACCATC+TGG + chr4.4:3938368-3938387 MS.gene20170:CDS 35.0%
GAGACAGAGAAGTGAAATTT+GGG - chr4.4:3938154-3938173 None:intergenic 35.0%
TCATGAGAAGCTAAAGATTG+CGG + chr4.4:3938316-3938335 MS.gene20170:CDS 35.0%
TGAGACAGAGAAGTGAAATT+TGG - chr4.4:3938155-3938174 None:intergenic 35.0%
AGGATCTTATGTTGAATCGG+CGG + chr4.4:3938427-3938446 MS.gene20170:CDS 40.0%
CAATTCTTCCAAGAAATCCG+CGG + chr4.4:3938089-3938108 MS.gene20170:CDS 40.0%
GGATTTCTTGGAAGAATTGC+CGG - chr4.4:3938088-3938107 None:intergenic 40.0%
GGTAAGTATGATGAGATCGT+GGG + chr4.4:3938512-3938531 MS.gene20170:CDS 40.0%
GGTGGCTTCGATAACAAAAA+CGG - chr4.4:3937778-3937797 None:intergenic 40.0%
GTGGCTTCGATAACAAAAAC+GGG - chr4.4:3937777-3937796 None:intergenic 40.0%
TAGTAATTCACCATCTGGGA+TGG + chr4.4:3938373-3938392 MS.gene20170:CDS 40.0%
TGAAGATTTGGCCATGGATT+TGG - chr4.4:3937805-3937824 None:intergenic 40.0%
! ATGGCGATTGTGGATTGTAA+TGG + chr4.4:3938392-3938411 MS.gene20170:CDS 40.0%
ACGGCAAATGAGTGGAGATT+GGG - chr4.4:3938041-3938060 None:intergenic 45.0%
CGGTAAGTATGATGAGATCG+TGG + chr4.4:3938511-3938530 MS.gene20170:CDS 45.0%
CTATCCAATGGCGTTTGCAA+CGG + chr4.4:3938284-3938303 MS.gene20170:CDS 45.0%
GAAATTTGGGTCAGATTCGG+AGG - chr4.4:3938141-3938160 None:intergenic 45.0%
GAAGCTAAAGATTGCGGCTA+TGG + chr4.4:3938322-3938341 MS.gene20170:CDS 45.0%
GAGAGTGAGGGATGAAGATT+TGG - chr4.4:3937817-3937836 None:intergenic 45.0%
GCGGCGTTTAATCCTAGTTT+GGG + chr4.4:3938446-3938465 MS.gene20170:CDS 45.0%
GGAGCTATGGTGAAACAAGA+GGG + chr4.4:3938557-3938576 MS.gene20170:CDS 45.0%
TCGTGTGGTCCAAATCTGTA+AGG - chr4.4:3938185-3938204 None:intergenic 45.0%
! AAAAACAGAGGAGCGTGTTG+AGG - chr4.4:3938215-3938234 None:intergenic 45.0%
! AAAACAGAGGAGCGTGTTGA+GGG - chr4.4:3938214-3938233 None:intergenic 45.0%
! TCAACACGCTCCTCTGTTTT+TGG + chr4.4:3938214-3938233 MS.gene20170:CDS 45.0%
! TTTGGCCATGGATTTGGCTT+CGG - chr4.4:3937799-3937818 None:intergenic 45.0%
!!! TTTTTGGAACCACGCACCAA+CGG + chr4.4:3938230-3938249 MS.gene20170:CDS 45.0%
AATTTCCACGTCGGAGCTGT+CGG + chr4.4:3937897-3937916 MS.gene20170:CDS 50.0%
ACGTGGAAATTGGAGATGGG+TGG - chr4.4:3937888-3937907 None:intergenic 50.0%
AGCTGTCGGATTATCCCCAT+CGG + chr4.4:3937911-3937930 MS.gene20170:CDS 50.0%
ATCACCGTTGCAAACGCCAT+TGG - chr4.4:3938291-3938310 None:intergenic 50.0%
CCATCGGGTCGGATCTTAAT+CGG + chr4.4:3937927-3937946 MS.gene20170:CDS 50.0%
CCCATCTCCAATTTCCACGT+CGG + chr4.4:3937888-3937907 MS.gene20170:CDS 50.0%
CCGACGTGGAAATTGGAGAT+GGG - chr4.4:3937891-3937910 None:intergenic 50.0%
CGATTAAGATCCGACCCGAT+GGG - chr4.4:3937929-3937948 None:intergenic 50.0%
CGGAGCTATGGTGAAACAAG+AGG + chr4.4:3938556-3938575 MS.gene20170:CDS 50.0%
CGTGGATGGAGTGATGAAGA+GGG - chr4.4:3937977-3937996 None:intergenic 50.0%
GAAATTGGAGATGGGTGGTC+GGG - chr4.4:3937883-3937902 None:intergenic 50.0%
GACGGCAAATGAGTGGAGAT+TGG - chr4.4:3938042-3938061 None:intergenic 50.0%
GAGGGATGAAGATTTGGCCA+TGG - chr4.4:3937811-3937830 None:intergenic 50.0%
GATTAAGATCCGACCCGATG+GGG - chr4.4:3937928-3937947 None:intergenic 50.0%
GCTAAAGATTGCGGCTATGG+AGG + chr4.4:3938325-3938344 MS.gene20170:CDS 50.0%
GGAAATTGGAGATGGGTGGT+CGG - chr4.4:3937884-3937903 None:intergenic 50.0%
GGAGTCAATGTCGAATTCCC+AGG + chr4.4:3937948-3937967 MS.gene20170:CDS 50.0%
GGCGGCGTTTAATCCTAGTT+TGG + chr4.4:3938445-3938464 MS.gene20170:CDS 50.0%
GTGCGTGGTTCCAAAAACAG+AGG - chr4.4:3938227-3938246 None:intergenic 50.0%
TCCGACGTGGAAATTGGAGA+TGG - chr4.4:3937892-3937911 None:intergenic 50.0%
TCCTAGTTTGGGTCCTCTTC+AGG + chr4.4:3938457-3938476 MS.gene20170:CDS 50.0%
TGTTGAGGGAGAAGATCGTG+TGG - chr4.4:3938200-3938219 None:intergenic 50.0%
TTTGAATATCGGGTGCTCCG+CGG - chr4.4:3938109-3938128 None:intergenic 50.0%
! CGGAAGAAGCTTTAACCCGT+TGG - chr4.4:3938249-3938268 None:intergenic 50.0%
! GACAAGTGATGGGAGGAGTT+GGG - chr4.4:3937841-3937860 None:intergenic 50.0%
! GCTGTGTTGGTGGAGAAAGA+CGG + chr4.4:3938536-3938555 MS.gene20170:CDS 50.0%
! TGACAAGTGATGGGAGGAGT+TGG - chr4.4:3937842-3937861 None:intergenic 50.0%
!!! TTTTGGAACCACGCACCAAC+GGG + chr4.4:3938231-3938250 MS.gene20170:CDS 50.0%
AGCCACCGAAGCCAAATCCA+TGG + chr4.4:3937791-3937810 MS.gene20170:CDS 55.0%
AGTGATGAAGAGGGAGGCCT+GGG - chr4.4:3937968-3937987 None:intergenic 55.0%
AGTGGAGATTGGGTTCGTCG+TGG - chr4.4:3938031-3938050 None:intergenic 55.0%
ATAATCCGACAGCTCCGACG+TGG - chr4.4:3937905-3937924 None:intergenic 55.0%
CCGATTAAGATCCGACCCGA+TGG - chr4.4:3937930-3937949 None:intergenic 55.0%
CGTGGAGGGAGAGATTTGTG+AGG - chr4.4:3938013-3938032 None:intergenic 55.0%
CTCATCATACTTACCGCCGC+CGG - chr4.4:3938507-3938526 None:intergenic 55.0%
CTTGGAAGAATTGCCGGCAG+TGG - chr4.4:3938082-3938101 None:intergenic 55.0%
GACAGCTCCGACGTGGAAAT+TGG - chr4.4:3937898-3937917 None:intergenic 55.0%
GAGAAACTGTTCGGCGTGGA+TGG - chr4.4:3937991-3938010 None:intergenic 55.0%
GAGATTGGGTTCGTCGTGGA+GGG - chr4.4:3938027-3938046 None:intergenic 55.0%
GAGGAGTTGGGTGAGAGTGA+GGG - chr4.4:3937829-3937848 None:intergenic 55.0%
GCCTGAAGAGGACCCAAACT+AGG - chr4.4:3938461-3938480 None:intergenic 55.0%
GCGTGGATGGAGTGATGAAG+AGG - chr4.4:3937978-3937997 None:intergenic 55.0%
GCTGTCGGATTATCCCCATC+GGG + chr4.4:3937912-3937931 MS.gene20170:CDS 55.0%
GGATGGAGTGATGAAGAGGG+AGG - chr4.4:3937974-3937993 None:intergenic 55.0%
GGTGGAGAAAGACGGAGCTA+TGG + chr4.4:3938544-3938563 MS.gene20170:CDS 55.0%
TCCACAATCGCCATCCCAGA+TGG - chr4.4:3938386-3938405 None:intergenic 55.0%
TCGGATTATCCCCATCGGGT+CGG + chr4.4:3937916-3937935 MS.gene20170:CDS 55.0%
TGAGGAGAAACTGTTCGGCG+TGG - chr4.4:3937995-3938014 None:intergenic 55.0%
TTTGGGTCCTCTTCAGGCTG+CGG + chr4.4:3938463-3938482 MS.gene20170:CDS 55.0%
! ACCATCTGGGATGGCGATTG+TGG + chr4.4:3938382-3938401 MS.gene20170:CDS 55.0%
! AGCTTTAACCCGTTGGTGCG+TGG - chr4.4:3938242-3938261 None:intergenic 55.0%
! GTGGTGGCGTTTATTGTCGC+CGG + chr4.4:3938485-3938504 MS.gene20170:CDS 55.0%
! TGAGATCGTGGGTGCTGTGT+TGG + chr4.4:3938523-3938542 MS.gene20170:CDS 55.0%
AATTGCCGGCAGTGGCCACA+GGG - chr4.4:3938074-3938093 None:intergenic 60.0%
GAGTGATGAAGAGGGAGGCC+TGG - chr4.4:3937969-3937988 None:intergenic 60.0%
GGAGATTGGGTTCGTCGTGG+AGG - chr4.4:3938028-3938047 None:intergenic 60.0%
GGAGGAGTTGGGTGAGAGTG+AGG - chr4.4:3937830-3937849 None:intergenic 60.0%
GGGCGGAGGTGACAAGTGAT+GGG - chr4.4:3937851-3937870 None:intergenic 60.0%
GTGGCGTTTATTGTCGCCGG+CGG + chr4.4:3938488-3938507 MS.gene20170:CDS 60.0%
TGGGCGGAGGTGACAAGTGA+TGG - chr4.4:3937852-3937871 None:intergenic 60.0%
! CGGAGGTGACAAGTGATGGG+AGG - chr4.4:3937848-3937867 None:intergenic 60.0%
! GATCGTGGGTGCTGTGTTGG+TGG + chr4.4:3938526-3938545 MS.gene20170:CDS 60.0%
! GGCCATGGATTTGGCTTCGG+TGG - chr4.4:3937796-3937815 None:intergenic 60.0%
CGGCGGAGACGGCAAATGAG+TGG - chr4.4:3938049-3938068 None:intergenic 65.0%
CGGGTGCTCCGCGGATTTCT+TGG - chr4.4:3938100-3938119 None:intergenic 65.0%
GAATTGCCGGCAGTGGCCAC+AGG - chr4.4:3938075-3938094 None:intergenic 65.0%
GCGTTTATTGTCGCCGGCGG+CGG + chr4.4:3938491-3938510 MS.gene20170:CDS 65.0%
TCCTCTTCAGGCTGCGGTGG+TGG + chr4.4:3938469-3938488 MS.gene20170:CDS 65.0%
GCCACCACCGCAGCCTGAAG+AGG - chr4.4:3938473-3938492 None:intergenic 70.0%
GGAGATGGGTGGTCGGGCGA+GGG - chr4.4:3937877-3937896 None:intergenic 70.0%
GGGTCCTCTTCAGGCTGCGG+TGG + chr4.4:3938466-3938485 MS.gene20170:CDS 70.0%
GGGTGGTCGGGCGAGGGATT+GGG - chr4.4:3937871-3937890 None:intergenic 70.0%
TGGAGATGGGTGGTCGGGCG+AGG - chr4.4:3937878-3937897 None:intergenic 70.0%
TGGGTGGTCGGGCGAGGGAT+TGG - chr4.4:3937872-3937891 None:intergenic 70.0%
TGGTCGGGCGAGGGATTGGG+CGG - chr4.4:3937868-3937887 None:intergenic 70.0%
CCGCTCCCTGTGGCCACTGC+CGG + chr4.4:3938066-3938085 MS.gene20170:CDS 75.0%
CCGGCAGTGGCCACAGGGAG+CGG - chr4.4:3938069-3938088 None:intergenic 75.0%
GCAGTGGCCACAGGGAGCGG+CGG - chr4.4:3938066-3938085 None:intergenic 75.0%
GCCACAGGGAGCGGCGGAGA+CGG - chr4.4:3938060-3938079 None:intergenic 75.0%
TCGGGCGAGGGATTGGGCGG+AGG - chr4.4:3937865-3937884 None:intergenic 75.0%
! GCCGTCTCCGCCGCTCCCTG+TGG + chr4.4:3938056-3938075 MS.gene20170:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 3937765 3938655 3937765 ID=MS.gene20170
chr4.4 mRNA 3937765 3938655 3937765 ID=MS.gene20170.t1;Parent=MS.gene20170
chr4.4 exon 3937765 3938655 3937765 ID=MS.gene20170.t1.exon1;Parent=MS.gene20170.t1
chr4.4 CDS 3937765 3938655 3937765 ID=cds.MS.gene20170.t1;Parent=MS.gene20170.t1
Gene Sequence

>MS.gene20170

ATGGATCGACCCGTTTTTGTTATCGAAGCCACCGAAGCCAAATCCATGGCCAAATCTTCATCCCTCACTCTCACCCAACTCCTCCCATCACTTGTCACCTCCGCCCAATCCCTCGCCCGACCACCCATCTCCAATTTCCACGTCGGAGCTGTCGGATTATCCCCATCGGGTCGGATCTTAATCGGAGTCAATGTCGAATTCCCAGGCCTCCCTCTTCATCACTCCATCCACGCCGAACAGTTTCTCCTCACAAATCTCTCCCTCCACGACGAACCCAATCTCCACTCATTTGCCGTCTCCGCCGCTCCCTGTGGCCACTGCCGGCAATTCTTCCAAGAAATCCGCGGAGCACCCGATATTCAAATCATCATTACCTCCGAATCTGACCCAAATTTCACTTCTCTGTCTCATTTTCTTCCTTACAGATTTGGACCACACGATCTTCTCCCTCAACACGCTCCTCTGTTTTTGGAACCACGCACCAACGGGTTAAAGCTTCTTCCGTTAACAACAGAGAAACTATCCAATGGCGTTTGCAACGGTGATTCGCTTCATGAGAAGCTAAAGATTGCGGCTATGGAGGCTGCGAATAAATCGCATGCTCCTTATAGTAATTCACCATCTGGGATGGCGATTGTGGATTGTAATGGAAAAATCTATAAAGGATCTTATGTTGAATCGGCGGCGTTTAATCCTAGTTTGGGTCCTCTTCAGGCTGCGGTGGTGGCGTTTATTGTCGCCGGCGGCGGTAAGTATGATGAGATCGTGGGTGCTGTGTTGGTGGAGAAAGACGGAGCTATGGTGAAACAAGAGGGCACTGTTAAATTGTTGCTTGAAACTATTTCTCCTAAATGTCAATTGCAGACTTTACTCTGTTCCTTTGATGAATGA
Protein sequence

>MS.gene20170.t1

MDRPVFVIEATEAKSMAKSSSLTLTQLLPSLVTSAQSLARPPISNFHVGAVGLSPSGRILIGVNVEFPGLPLHHSIHAEQFLLTNLSLHDEPNLHSFAVSAAPCGHCRQFFQEIRGAPDIQIIITSESDPNFTSLSHFLPYRFGPHDLLPQHAPLFLEPRTNGLKLLPLTTEKLSNGVCNGDSLHEKLKIAAMEAANKSHAPYSNSPSGMAIVDCNGKIYKGSYVESAAFNPSLGPLQAAVVAFIVAGGGKYDEIVGAVLVEKDGAMVKQEGTVKLLLETISPKCQLQTLLCSFDE